649results about How to "Low detection cost" patented technology

The invention relates to a technology for performing foundation pile quality detection and geology survey by adopting a single tube longitudinal wave method. The technology is relevant to comprehensive testing of the actual using effect of a foundation pile and is characterized in that longitudinal wave transmitting downwards along a pile body is generated by knocking the top surface of the foundation pile, the longitudinal wave velocity and pile length of the foundation pile can be accurately measured by detecting the time difference of arrival of the longitudinal wave in a sounding pipe, and the quality of pile body concrete can be judged; actual measurement on the time difference of arrival of the longitudinal wave which is transmitted from the pile side soil along the knocked pile body can be conducted in adjacent pipelines or geological bore holes, and the length of the foundation pile, the pile body quality and the chambering degree can be measured; and the wave length of a geological soil layer can be measured, the condition of the geological soil layer around the pile can be judged, and the rock-inlet condition of the foundation pile can be analyzed. By means of the technology for performing foundation pile quality detection and geology survey by adopting the single tube longitudinal wave method, a signal processor can be configured externally or inserted in on the basis of the existing low-strain ultrasonic detection devices and the like, the technology can achieve foundation pile quality nondestructive testing and geology survey, is an economic and ready-made pile foundation nondestructive testing auxiliary validation method and can remarkably improve accuracy and service level of pile foundation quality decision.

The invention discloses a device for the rapid NDE (nondestructive examination) of component contents of green tea, which mainly comprises a photoelectric signal examination system and an embedded DSP (Digital Signal Processing) system, wherein the photoelectric signal examination system and the embedded DSP system are both capsulated in an outer casing. The invention can realize the NDE of routine examination items in the green tea, such as amino acid, caffeine, tea polyphenol, moisture, and the like, is rapider and more environment-friendly than the routine physical and chemical inspection of green tea components without carrying out sample pretreatment, and has good examination result repetitiveness and low examination cost.

The invention discloses a kit and method for staphylococcus aureus CRISPR locus detection. The kit comprises three pairs of specific primers designed according to three CRISPR locus gene sequences of staphylococcus aureus. The invention further discloses the method for staphylococcus aureus CRISPR locus detection. The three pairs of specific primers are utilized to amplify corresponding CRISPR fragments from sample genome DNAs. The kit and the detection method are simple to operate, high in sensitivity and specificity and low in detection cost and have good popularization and application values.

The invention relates to the technical fields of biological detection, environment monitoring and clinical detection, in particular to a micro-fluidic chip and a manufacturing method thereof, which are used for overcoming the defects of large sample using amount and complex operating steps existing in the biological detection process in the prior art. The invention provides a micro-fluidic chip and a manufacturing method thereof. The micro-fluidic chip comprises a substrate layer, a functional layer and a micro-fluidic pipeline layer, wherein the functional layer is fixed on the substrate layer, and comprises at least two parallel functional strips; the functional strips are strip-shaped bands formed by a material which can react with a specific material; the micro-fluidic pipeline layer is provided with at least two pipelines; the pipelines are communicated with at least one functional strip; and the two ends of each pipeline are provided with through holes which are communicated with the atmospheric air. According to the micro-fluidic chip provided by the invention, various different substances in a plurality of samples can be detected simultaneously. A detection method provided by the invention has the advantages of easiness for operating, small reagent using amount, saving in time, high efficiency and accuracy.

Microflow controlled chip 14 is prepared by sticking etched two layers of sheets. Chunnel 21 in decussation is fluted between the said sheets. There are inhalant pore 17 of sample, opposite two inhalant pores 18 for buffer solution, and flowout pore for waste fluid at end point of channel 21 connected to outside. One electrode is inserted into the four pores respectively. Laser emitted from laser source 1 is focused on testing point 24 on the channel 21 in decussation. Fluorescence emitted from matter in channel 21 and received by optical fiber is transferred to spectrum detection system 16 composed of CCD spectrographic detector. The invention possesses features of small size, lightweight, compact structure, and simple operation, as well as optional fluorescent reagent, fast response speed and fluorescence intensities in multiple wavelengths obtained. The invention is applicable to areas such as analyzing biochemistry matter, drug screening and clinical diagnosis.

The invention relates to an on-site detection device and a detection method for heat transfer coefficients of building enclosure structures. The device comprises a temperature difference producing device arranged on a to-be-detected enclosure structure, as well as a data acquisition device for testing and receiving temperature and heat flow of the inner and outer sides of the to-be-detected enclosure structure. The device is characterized in that the temperature difference producing device is arranged in a bucket-shaped shell; the shell and the to-be-detected enclosure structure form a cylindrical cavity; a fan used for forced convection is arranged in the shell; and the temperature difference producing device comprises heating equipment and refrigeration equipment. The detection device provided by the invention enables heat to be capable of one-dimensional conduction, thereby greatly reducing measurement errors. The device used for on-site detection has the advantages of convenient installation and use, as well as testing time free from seasonal limit.

The invention discloses a method for testing defects of a single membrane electrode assembly in a fuel cell stack, which comprises that: two electrodes of a constant current source are connected with an anode and a cathode of the fuel cell stack; a single acquisition fixture of a voltage detecting system is fixed on the fuel cell stack; the temperature of the fuel cell stack is controlled to be between 60 and 75 DEG C; two sides of an MEA of a fuel cell are introduced with saturated humidifying hydrogen at a flow rate of between 0.1 and 10 L/min, the current of the constant current source is adjusted to be between 1 and 10A after 10 to 20 minutes, and voltage data of each membrane electrode assembly is acquired after 4 to 10 minutes to calculate a voltage average; and the defective membrane electrode assembly is determined by comparing the voltage data of the single membrane electrode assembly and the voltage average. The method has the advantages of simple and easy implementation and accurate test result, and belongs to the field of fuel cell internal resistance test.

The invention discloses a quick African swine fever virus detection card and application thereof, so as to solve the technical problem of hard African swine fever virus detection. Polyclonal antibodies PoAbI against African swine fever virus p30, P54 and p72 protein labeled with colloidal gold are absorbed on a colloidal gold labeling pad of the detection card; a detection membrane is provided with goat or rabbit anti-mouse IgG antibodies or a quality control line C printed by staphylococcus aureus SPA; and a detection line T printed by polyclonal antibodies PoAbII against African swine fevervirus p30, P54 and p72 protein is also arranged. The invention also discloses a using method for the quick African swine fever virus detection card. The method comprises steps: a to-be-detected sampleis acquired, and PBS or water is added for suspension or grinding; the sample is dripped to the sample loading end of the quick African swine fever virus detection card; and horizontal placing is carried out to observe a result. The quick African swine fever virus detection card adopts a double antibody sandwich method, and has the advantages of strong specificity, high sensitivity, high detection efficiency, high efficiency and practicability, and important practical application value.

The invention discloses a detection method for the static load of a pile foundation, which comprises the following steps of: 1, installation: pre-embedding more than one anti-pulling draw bar at the top of a pile foundation to be detected and connecting the anti-pulling draw bars with the pile foundation to be detected into a whole, and sticking a carbon fiber layer on the outer wall of the pile foundation to be detected, wherein the height for sticking the carbon fiber layer on the pile foundation to be detected is greater than or equal to the length of the anti-pulling draw bar embedded in the pile foundation to be detected; 2, connection: connecting the top of the anti-pulling draw bar with a detection component through a draw bar sleeve; and 3, detection: measuring and recording the original elevation of the pile foundation to be detected, and applying force to the anti-pulling draw bar through the detection component; recording the elevation change condition of the pile foundation to be detected each time one-level load is added according to the force loading order; and after the force is completely loaded, obtaining the static load condition of the pile foundation according to the obtained data. The detection method has advantages of simple principle, easy operation, high safety, high detection precision, wide application range, high detection efficiency, time saving and economy.

A method for quantitatively testing the water quality in oil field by use of sulfate reducing bacterium (SRB) includes taking 3-5 parallel samples in same dilution gradient, filling them in culture media, putting the MPN culture media on shaker, shaking at 38 deg.C and 180 rpm and respective counting in the fourth and the seventh days.

A line-of-sight optical detection system comprises: a plurality of dimmable light sources (11, 12, 13, 14); a controller (30) for controlling the light sources to emit coded light; a detector (21, 22, 23, 24), receiving light from two or more of said light sources. The controller decodes the detector output signal, determines which light source contributes to the light received by the detector and, on the basis of the outcome, determines a location of an object (2). A communication system (100) comprises:—a plurality of dimmable light sources (111, 112, 113, 114); a controller (130) for controlling the light sources to emit coded light; a receiver (200) comprising a CPU (230) and a light detector (211), receiving light from at least one of said light sources. The CPU (230) decodes the detector output signal and, on the basis of the outcome, decides on an action to be taken.

The invention relates to a multiple fluorescence PCR (polymerase chain reaction) kit capable of detecting five DNA (deoxyribonucleic acid) viruses of an aquatic animal simultaneously. The kit comprises a buffer solution, and also comprises five pairs of fluorescence PCR primers and five molecular beacon probes and a PCR primer, wherein the five pairs of fluorescence PCR primers and the five molecular beacon probes are respectively corresponding to channel catfish virus, epizootic haematopoietic necrosis virus, goldfish haematopoietic necrosis virus, fancy carp herpes virus and red porgy iridescent virus, the fluorescence PCR primers and the molecular beacon probes comprise nucleotide sequences shown in SEQ ID NO.3-17, and the PCR primer comprises nucleotide sequences shown in SEQ ID NO.1-2. The multiple fluorescence PCR kit capable of detecting five DNA viruses of the aquatic animal simultaneously can rapidly detect all the DNA viruses infecting the aquatic animals once, the sensitivity is increased, and the cost for detecting single sample is reduced.

The invention relates to a method to testing formaldehyde rapidly that uses water or formaldehyde developer as liquid extract, taking extract to the releasing material from the tested sample lasting for at least 3 minutes, and the microwave frequency range is 1GHz-30GHz. Adding formaldehyde developer into the extract liquid of tested sample, mixing to equal, heating the extract liquid and observing the alteration of the color to take qualitative analysis whether the liquid contains formaldehyde and determining whether the sample contains formaldehyde. By comparing the color of the liquid with standard color, the content of the sample would be measured. The invention has short testing time, low const and would be used in and locale.'

The invention provides a fast multiplex PCR identification diagnosis detection method of invasive fungus infection based on cfDNA (cell free DNA). The method can be used for identifying the invasive infection caused by clinic common and high-incidence Candida albicans, Candida tropicalis, Candida parapsilosis, Candida krusei, Candida glabrata and Aspergillus fumigtus. An amplification primer is designed according to characteristic genome segments of each fungus category and species; a detection fluorescence probe of a strain can be distinguished according to the amplification segment design; the real time PCR can be performed on a sample to be tested; high sensitivity of nested PCR and high specificity and multi-target performance advantages of multiple fluorescent hybrid probe PCR are integrated for identifying the fungus strain. The invention also provides a PCR diagnosis kit for the invasive fungus infection and application thereof.

The invention discloses a rhodamine B-based multifunctional fluorescent probe for recognizing Fe<3+>, Al<3+> and Cr<3+> ions and a preparation method and application of the rhodamine B-based multifunctional fluorescent probe for recognizing the Fe<3+>, Al<3+> and Cr<3+> ions. The rhodamine B-based multifunctional fluorescent probe is shown as the specific structural formula below, wherein R refers to the description below. The rhodamine B-based multifunctional fluorescent probe recognizes the trivalent metal ions including Fe<3+>, Al<3+> and Cr<3+> in methanol. The rhodamine B-based multifunctional fluorescent probe for recognizing the Fe<3+>, Al<3+> and Cr<3+> ions, the preparation method and application have the advantages that the rhodamine B-based multifunctional fluorescent probe can be used for recognizing the trivalent metal ions including Fe<3+>, Al<3+> and Cr<3+>, thereby being multifunctional; the rhodamine B-based multifunctional fluorescent probe is excellent in selectivity when recognizing the Fe<3+>, Al<3+> and Cr<3+>, is high in anti-interference capability and is unaffected by other divalent metal ions; the rhodamine B-based multifunctional fluorescent probe which is a fluorescence-enhanced fluorescent probe is high in detection sensitivity.

The invention relates to a primer, a probe and a method for detecting entomophily or contact transmission pathogens by using a liquid phase chip, which are used for detecting nine clinical common entomophily or contact transmission infectious disease pathogens. The invention can detect the nine clinical common entomophily or contact transmission infectious disease pathogens based on an MASA (multi-analyte suspension array) liquid phase chip technology, homology analysis is performed respectively according to all nucleotide sequences of 9 target viruses which can be retrieved in a gene bank mainly, the degenerate primer and the specific probe are designed, two turns of PCR (polymerase chain reaction) and molecular hybridization are further performed, and a Luminex100 system is further usedfor detection, thereby determining types of the pathogens contained in the sample. The invention is most important for adopting a correct treatment scheme and timely taking measures for preventing disease transmission by detection and early diagnosis of the 9 clinical common entomophily or contact transmission infectious disease pathogens. The invention has the advantages of fast detection speed,simpleness in operation, high sensitivity, good specificity and the like, and is conductive to popularization.

The invention discloses a multi-residue colloidal-gold rapid detection kit and a detection method and application thereof, belonging to the field of immunology. The kit provided by the invention mainly comprises a multi-residue colloidal-gold rapid detection card; the detection card comprises a detector bar and a plastic card shell, and the detector bar is composed of a sample pad, a colloidal-gold antibody binding pad, a nitrocellulose membrane and a water absorbing pad. The colloidal-gold antibody binding pad comprises a colloidal gold labeled gentamycin monoclonal antibody, a colloidal gold labeled kanamycin monoclonal antibody and a colloidal gold labeled fluoroquinolone monoclonal antibody. The central part of the nitrocellulose membrane is coated with a detection line and a control line, wherein the detection line is arranged to be a protein conjugate and the control line is a goat anti-mouse IgG monoclonal antibody. The rapid detection kit provided by the invention can realize simultaneous detection of a plurality of residues only through simple pre-treatment, the whole operation process is simple, the kit is convenient to carry, result determination is rapid and accurate, and the kit is applicable to on-site monitoring and to qualitative screening of considerable samples.

The invention provides a digital double-micro speckle measuring method for strain. The invention relates to the measuring method for the stress and the strain of a structure, in particular to the measuring method for the stain with application of the photoelectron technology. The CCD with a micro-lens is arranged on a fixed platform while two CCDs serve as a group in the invention; when in measuring, a speckle field is manufactured on the surface of the measured object and the speckle images of the measured object in the scale distance between two points before and after being loaded are collected by the double-CCD synchronously and then are operated by the related processing software after being transmitted into a PC machine, at last, the mechanics parameter of the measured object can be obtained. The invention has short detection period, low consumption of resources, low detection cost, good economic benefit, easy interpretation and convenient and wide application. Meanwhile, the method can be used for detecting and evaluating the safety performance of other large buildings, such as houses, large dams, etc.

The invention relates to a method for measuring antigen protein in serum by chemiluminescence protein chips and a kit, which belongs to the medical detection technology. The method is characterized in that a chemiluminescence technology, a standard curve and a protein chip technology are combined for performing quantitative determination of certain antigen protein substance in serum, the high specialty and sensitivity of the chemiluminescence technology and the standard curve are employed for realizing the quantitative determination; the protein chip technology can realize simultaneous detection and analysis of several samples, so that the detection time consumption of a whole set ofe sample is greatly reduced, and the method has the characteristics of reliability, accuracy and low cost; and the method is suitable for general analysis of common antigen protein level in serum and quantitative analysis of great disease antigen marks, and the method is an medical auxiliary detection method having great meaning. The invention also provides a matched kit based on the method.

The invention relates to a tetracyclines aptamer, an aptamer electrochemical biological sensor for detection of tetracyclines, and a preparation method and a detection method of the sensor. The tetracyclines aptamer is a single strand DNA probe containing a TTGAGCCCT sequence and having the length of 13-20 bp, and an amino is modified by a 5' end, namely 5'-NH2-(CH2)6-. The aptamer electrochemical biological sensor for the detection of the tetracyclines contains the tetracyclines aptamer. The sensor improves the sensitivity of the detection of the tetracyclines, and can detect the tetracyclines in a minimum concentration of 1.0 ng/mL; and the sensor greatly reduces the cost of the detection of the tetracyclines, has simple and convenient operation, and can rapidly detecting the tetracyclines.