13results about How to "Low detection cost" patented technology

The invention relates to a molecular marker which is located at the 47023718th basic group from the 5tail end on a No.16 chromosome of a pig reference genome Sscrofa11.1 and is related to a pig internal farrowing litter weight standard deviation. The invention describes a primer pair and a kit for detecting the molecular marker, and application of the primer pair and the kit in identifying the size of the standard deviation of the litter weight in the pig, screening pig varieties or genetic breeding of the pig. The pig internal litter weight standard deviation of the AA genotype individual is 2.79 kg, the pig internal litter weight standard deviation of the GG genotype individual is 4.38 kg, and the AA genotype individual is obviously smaller than the GG type individual, namely, the pig resilience of the AA genotype individual is higher than that of the GG individual. The AA type individuals are selected, and the pigs with small standard deviation of litter weight and high restoring force in the pigs can be obtained. According to the method, the problem of determining the pig resilience in actual production is effectively solved, the breeding cost is reduced, the pig internal litter weight standard deviation is effectively reduced, so that the pig resilience is improved, the method is high in accuracy and low in detection cost, automatic detection can be realized, and the method has very high practical application value in the aspect of pig breeding.

Glial fibrillary acid protein detection kit. The present invention relates to a kit for diagnosing markers of neurodegenerative diseases (particularly Alzheimer's disease), and in particular to a kit for detecting glial fibrillary acid protein (GFAP), wherein the kit detects GFAP based on single molecule detection technology. In addition, it also relates to a single molecule detection system for detecting GFAP. The present invention can achieve extremely high sensitivity while performing rapid detection, and the kit has high stability, a simple system, and low cost.

This invention provides a method for detecting AAV antibody titers, belonging to the field of biomedical technology, comprising: S1 preparing serum-free AAV vectors with different MOI values; preparing a mixture containing different volumes of test serum samples and AAV vectors with known MOI values ​​for co-incubation; S2: adding plasmids containing the CRISPR-Cas9 gene editing system to electroporation buffer, performing electroporation on a known number of cells, adding the serum-free AAV vectors and mixture from step S1 to the electroporated cells respectively, and detecting the expression efficiency of the reporter gene after electroporation; S3: calculating the AAV antibody titer in the test serum sample based on the detection results. The main advantage of this invention is that it can display AAV antibody titers digitally and quantitatively, facilitating understanding and communication, and obtaining stable and reliable results using different cell lines and different AAV transduction doses. This method is easy to standardize and validate, saving time, effort, and detection costs compared to traditional methods, and directly reflects antibody neutralizing activity.

The application discloses a method for accurately analyzing high-temperature change of niobium compound in steel, which comprises the following steps: (1) fixing a metallographic sample on a clamp; (2) polishing and cleaning the surface of the metallographic sample; (3) observing the metallographic sample by using a metallographic microscope, finding characteristic niobium compound, marking the outer edge of the characteristic niobium compound by using a microhardness tester, and then accurately measuring the size of the marked niobium compound under a scanning electron microscope and accurately determining the composition of the marked niobium compound by using an energy spectrometer; (4) heat treating the metallographic sample to obtain a heat-treated sample; (5) removing the surface oxide of the heat-treated sample and then corroding the surface of the heat-treated sample; and (6) observing the change of the marked niobium compound after heat treatment under a scanning electron microscope and determining the composition of the marked niobium compound by using an energy spectrometer. The method has the characteristics of low detection cost, rapidness, simplicity, accuracy and the like.

The application belongs to the technical field of chemical analysis, and particularly relates to a detection method for D-glycophosphocholine content in L-alpha-glycophosphocholine and application. The detection method selects a silica gel surface polysaccharide covalent bonding cellulose type or silica gel surface polysaccharide coating cellulose type normal phase chromatographic column and an evaporative light scattering detector (ELSD), detects through an HPLC-ELSD method, has the advantages of simple operation, high sensitivity, good method specificity and reproducibility, is a high-efficiency liquid phase analysis method convenient for standardized operation, can completely separate L-alpha-glycophosphocholine from low-limit D-glycophosphocholine isomer impurities, and can accurately quantify.

The invention relates to a kit for screening dilated cardiomyopathy. The kit comprises a reagent for detecting one or more of genes of TP53, TNF, IL1beta, JUN, CD8A, TLR4, UBB, CTLA4, CXCL8 and NFKBIA. The kit provided by the invention is high in detection efficiency and low in detection cost, provides important reference basis for diagnosis and prognosis judgment of patients with dilated cardiomyopathy, and can remarkably improve the survival rate of the patients.

The utility model provides a conveying device for filter stick sampling, which comprises a collecting cylinder and a cover plate, the cover plate is connected with the end face of the upper end of the collecting cylinder, and the cover plate is used for sealing and covering the upper port of the collecting cylinder; at least two filter stick inlet pipes are arranged on the cover plate; each filter stick inlet pipe is connected with a filter stick conveying pipeline of each filter stick sampling device; the cover plate is further provided with an air inlet pipe used for being connected with an air supply system. The collecting barrel is of a detachable split structure, and a lower end opening of the collecting barrel is a filter stick outlet. According to the utility model, the same comprehensive test platform can be simultaneously butted with filter stick sampling devices of at least two forming machines, so that the number of the comprehensive test platforms can be effectively reduced, the utilization rate of the comprehensive test platforms is improved, and the detection cost of filter sticks is reduced.

A variation site combination related to salt tolerance of wheat, and detection products and applications thereof, belong to the field of molecular genetic breeding technology, the variation site combination is composed of 94 SNP sites and 1 Indel site, the physical position of each variation site is determined based on sequence alignment of wheat reference genome IWGSC RefSeq v.2.1, the Indel is located in the region of 540783807 to 540783852 of 5D chromosome, and is marked as 5D-Indel. The application also provides a nucleotide probe combination and a liquid gene chip developed based on the variation site combination. By using the above variation site combination and detection products, the salt tolerance related genotype of wheat germplasm resources and hybrid offspring can be rapidly screened in early breeding, the accurate identification and directional polymerization of salt tolerance excellent allelic variation can be realized, the dependence on field saline phenotype identification is reduced, the breeding cycle is shortened, and the detection cost and selection efficiency are considered.

This invention discloses an improved method for integrating electrocardiogram (ECG) and electroencephalogram (EEG) into one. Based on the existing ECG DB15 wiring sequence multiplexing circuit, it fully utilizes the reserved empty pins (NC pins) in the wiring sequence without changing the core definition of the original DB15 wiring sequence or affecting the original 12-lead ECG signal acquisition function. This achieves integrated reuse of the ECG / EEG interface and circuit, eliminating the need for an additional independent EEG interface and complex circuitry. Furthermore, this invention, through a built-in recognition module, can automatically identify whether the user has inserted an ECG or EEG cable and quickly switch the corresponding signal acquisition parameters, filtering modes, and data processing logic. This enables automatic switching between ECG and EEG acquisition functions without manual adjustment, making operation convenient and efficient. It effectively reduces the operational difficulty for medical personnel and minimizes detection errors caused by human error.

The invention provides a prostate cancer detection method and kit based on exosome RNA, and the method comprises the following steps: preparing nano-scale cationic lipid particles coated with RNA molecular beacons, each RNA molecular beacon is combined with a corresponding target exosome to generate fluorescence, and the fluorescence is used for detecting the prostate cancer. The target exosome is allowed to be one or more of the following exosomes in the urine: miR-141, miR-375, PCA3 and T1-E2; a porous chip plate is coated with an exosome capture system and nanoscale cationic lipid particles to form the detection kit; the exosome capturing system is used for capturing exosomes; a to-be-detected sample is pretreated, the pretreated to-be-detected sample is added into the kit, and whether a subject suffers from the prostate cancer or not is judged according to a fluorescence imaging result of the kit; wherein the to-be-detected sample at least comprises urine of a subject, non-invasive detection of prostatic cancer can be realized, the detection cost is low, the time is short, and the cost is relatively low.

The application discloses a method and system for assisting in determining the grade of brain glioma, and belongs to the technical field of biological genes and bioinformatics. The method comprises the following steps: obtaining sample information of a patient and pre-processing the sample; performing FISH detection, NGS detection assisted bioinformatics analysis and pathological detection on the sample to obtain N1 FISH detection indexes, N2 NGS detection indexes and N3 pathological detection indexes, and gene variation phenomenon characteristics corresponding to each detection index; and determining the type and grade of brain glioma based on the detection indexes and the gene variation phenomenon characteristics corresponding to one or more of the detection indexes. The application also discloses corresponding systems, electronic devices and computer readable storage media. The NGS sequencing technology is used to replace part of the conventional FISH detection mode, the NGS data is retained, secondary analysis is performed by using the bioinformatics method to obtain new molecular indexes in the guidelines, the detection types and times are reduced, and the detection mode is optimized.

The curbstone-free asphalt pavement construction method comprises the following steps: constructing a roadbed at a design position of an asphalt pavement, and enabling the top surface of the roadbed to be flush with the top surfaces of green belt planting soil on two sides; baffles are connected to the two sides, relatively close to green belt planting soil on the two sides, of the top of the roadbed, the baffles are arranged in the length direction of the roadbed in a full-length mode, and the top elevation of the baffles is flush with the top design elevation of the asphalt pavement; coarse aggregate asphalt is poured on the inner sides of the two baffles until reaching the designed position, soil is backfilled on the outer sides of the two baffles for the first time, and the soil body top elevation of the soil backfilled for the first time is flush with the top elevation of the coarse aggregate asphalt; fine aggregate asphalt is poured on the inner sides of the two baffles till the fine aggregate asphalt is flush with the top faces of the baffles, soil is backfilled on the outer sides of the two baffles for the second time, the elevation of the top of a soil body of the backfilled soil for the second time is flush with the top faces of the baffles, and through the construction method, the cost is saved, and the construction efficiency is improved.

The present invention belongs to the field of biomedicine technology, and discloses a marker for early screening of cardiac adenocarcinoma in high-risk populations, the marker being GAGE12B protein or a combination of GAGE12B protein and AGPAT4 protein or a combination of GAGE12B protein and P2RX4 protein, and by detecting the expression level of the above marker in serum, cardiac adenocarcinoma can be effectively detected. The present invention also discloses a test strip for early screening of cardiac adenocarcinoma in high-risk populations, the test strip binding pad is coated with a capture antibody, the capture antibody is a mixture of GAGE12B antibody A and AGPAT4 antibody A; two detection lines and a quality control line are provided on the chromatography pad, and detection antibodies are fixed on the detection lines, and the detection antibodies fixed on the two detection lines are GAGE12B antibody B and AGPAT4 antibody B respectively; rabbit anti-mouse IgG is fixed on the quality control line. The test strip of the present invention can quickly detect GAGE12B and AGPAT4 in human serum, and can effectively detect cardiac adenocarcinoma according to the detection results, greatly improving the detection rate of early cardiac adenocarcinoma.