29results about How to "Low detection cost" patented technology

The invention discloses an alfalfa SNP (Single Nucleotide Polymorphism) molecular marker combination and application thereof. The SNP molecular marker combination is composed of 61351 SNP molecular markers, the physical positions of the 61351 SNP molecular markers are determined based on sequence alignment of a medicago sativa reference genome ZM-4alfalfa gene, the SNP molecular markers are good in site specificity, high in universality and high in polymorphism, medicago sativa germplasms of different sources can be effectively distinguished, and the application prospect is wide. The alfalfa whole-genome liquid-phase gene chip prepared from the SNP molecular marker can be used for alfalfa germplasm resource genetic diversity evaluation, genetic map construction, functional gene positioning, whole-genome association analysis and the like, and provides important tools and technical supports for alfalfa molecular design breeding and variety authenticity identification. The method has important significance on high-quality development of alfalfa seed industry in China.

The invention discloses a method for displaying and observing aggregated niobium compounds causing stretching layering cracking of wheel steel. The method comprises the steps of sampling, grinding, low-power defect display, stripping, inlaying, grinding, polishing, corrosion, scanning electron microscope observation and phase component and element atomic ratio determination through an energy disperse spectroscopy. According to the method, the periphery is ground after sampling, then the segregation defect is displayed through corrosion of a low-power corrosive liquid, the sample is stripped off from the position 0.5-1 mm away from a segregation defect line through linear cutting, hot inlaying is conducted, and after grinding, polishing and corrosion are conducted, the shape, size and distribution of aggregated and distributed niobium compounds can be clearly displayed under a scanning electron microscope; and the components and atomic ratio of the phase can be determined by using an energy disperse spectroscopy.

The invention relates to a molecular marker which is located at the 47023718th basic group from the 5tail end on a No.16 chromosome of a pig reference genome Sscrofa11.1 and is related to a pig internal farrowing litter weight standard deviation. The invention describes a primer pair and a kit for detecting the molecular marker, and application of the primer pair and the kit in identifying the size of the standard deviation of the litter weight in the pig, screening pig varieties or genetic breeding of the pig. The pig internal litter weight standard deviation of the AA genotype individual is 2.79 kg, the pig internal litter weight standard deviation of the GG genotype individual is 4.38 kg, and the AA genotype individual is obviously smaller than the GG type individual, namely, the pig resilience of the AA genotype individual is higher than that of the GG individual. The AA type individuals are selected, and the pigs with small standard deviation of litter weight and high restoring force in the pigs can be obtained. According to the method, the problem of determining the pig resilience in actual production is effectively solved, the breeding cost is reduced, the pig internal litter weight standard deviation is effectively reduced, so that the pig resilience is improved, the method is high in accuracy and low in detection cost, automatic detection can be realized, and the method has very high practical application value in the aspect of pig breeding.

The invention discloses a method and a system for auxiliary determination and grading of brain glioma, and belongs to the technical field of biological genes and bioinformatics. The method comprises the following steps: acquiring sample information of a patient and preprocessing a sample; carrying out FISH detection, NGS detection auxiliary biological information analysis and pathological detection on the sample to respectively obtain N1 FISH detection indexes, N2 NGS detection indexes, N3 pathological detection indexes and gene variation phenomenon characteristics corresponding to the detection indexes; and judging the type and classification of the brain glioma based on the detection indexes and the gene variation phenomenon characteristics corresponding to one or more of the detection indexes. The invention further discloses a corresponding system, electronic equipment and a computer readable storage medium, the NGS sequencing technology is used for replacing a part of conventional FISH detection modes, meanwhile, NGS data are reserved, secondary analysis is carried out through a signal generation method to obtain guide newly-added molecular indexes, the detection types and times are reduced, and the detection modes are optimized.

The invention discloses a simple detection device and method for a breather valve, and the device comprises a hollow blind plate, a first detection gas circuit, and a second detection gas circuit, the hollow blind plate is disposed between the breather valve and a communication pipeline, the hollow blind plate is communicated with the inner cavity of the breather valve, the first detection gas circuit is communicated with the hollow blind plate, and is used for introducing gas into the breather valve, and the second detection gas circuit is communicated with the hollow blind plate. Simulating an expiration state of a breather valve; the second detection gas circuit is communicated with the hollow blind plate so as to form negative pressure for the breather valve and simulate the inspiration state of the breather valve; the first detection gas path and the second detection gas path are both communicated with the hollow blind plate through the monitoring gas path and are both communicated with the gas source. The whole device is simple in structural design, convenient to operate and high in popularization value, whether the breather valve is in a normal state or not is determined through regular self-testing, meanwhile, online self-testing can be achieved, and daily safe operation of the oil tank is not affected.

The invention provides a bridge tower reinforcing steel bar component virtual pre-assembling method, which comprises the following steps of: performing data analysis and modeling on contour point coordinates of upper, middle and lower sections of each section of a reinforcing steel bar component by adopting a BIM tool to form theoretical coordinate point data, and considering influence factors of a monitoring pre-bias value and concrete pouring deformation in a current actual construction stage to obtain a pre-assembling result of the reinforcing steel bar component; forming a segment theoretical data model; building an actually measured data model, acquiring forming precision information of the reinforcing steel bar component through various technologies of a total station, three-dimensional laser and image vision, and importing the forming precision information into a digital system to build a real-time data model; real-time comparative analysis is conducted, deviation visualization is achieved through comparative analysis of a theoretical model and a real-time model, size data of the next section of steel bar component is predicted through a special algorithm, the forming process of the next section of steel bar component is guided, and dynamic adjustment of production data is achieved; a set of tower column steel bar component virtual pre-assembly software is researched and developed, all the functions are integrated, and digital virtual construction is achieved.

The invention relates to the field of gear detection, in particular to a herringbone tooth centering detection method, which comprises the following steps of: selecting four measuring balls with consistent parameters, and measuring a herringbone tooth with an even number of teeth: respectively putting the two measuring balls into a first tooth groove of a left-handed tooth surface and a first tooth groove of a right-handed tooth surface, the other two measuring balls are respectively placed in the (m / 2) th tooth groove of the left-handed tooth surface and the right-handed tooth surface; connecting the central points of the two measuring balls on the same spiral tooth surface, and measuring an included angle between the connecting lines; the method comprises the following steps of: measuring a herringbone tooth with an odd number of teeth: respectively putting two measuring balls into first tooth grooves of a left-handed tooth surface and a right-handed tooth surface, respectively putting the other two measuring balls into (n + 1) / 2 tooth grooves of the left-handed tooth surface and the right-handed tooth surface, respectively connecting the central points of the two measuring balls positioned on the same-handed tooth surface, and measuring an included angle between the connecting lines; and if the measured included angle value is greater than the preset value, the herringbone teeth are not centered. By implementing the scheme, the detection cost of herringbone tooth centering detection is reduced, and the detection range is expanded.

Glial fibrillary acid protein detection kit. The present invention relates to a kit for diagnosing markers of neurodegenerative diseases (particularly Alzheimer's disease), and in particular to a kit for detecting glial fibrillary acid protein (GFAP), wherein the kit detects GFAP based on single molecule detection technology. In addition, it also relates to a single molecule detection system for detecting GFAP. The present invention can achieve extremely high sensitivity while performing rapid detection, and the kit has high stability, a simple system, and low cost.

The invention relates to a drug-resistant tuberculosis early warning method and kit for heterogeneity drug resistance semi-quantitative detection. The early warning method comprises the following steps: designing an amplification primer, a retardation probe and an extension primer for a target gene; extracting DNA of an object to be detected; adding the amplification primer and the retardation probe into a nucleic acid amplification system, and carrying out nucleic acid amplification on the DNA of the object to be detected; digesting an amplification product by using shrimp alkaline phosphatase (SAP); adding the extension primer into a system subjected to SAP digestion, and carrying out single base extension; and finally, carrying out resin desalination on the extension product, and detecting by using matrix-assisted laser desorption ionization-time-of-flight mass spectrometry. The invention also discloses a kit, which comprises an amplification primer, a retardation probe, an extension primer, an amplification reaction solution, a digestion reaction solution, an extension reaction solution, resin and a chip. According to the invention, trace mutation sites can be rapidly, efficiently and simply detected; and the proportion of mutation sites of a sample can be semi-quantitatively detected.

The present invention provides a molecular marker related to the water-power trait of pork and its application. The molecular marker is a g.152858710C>T polymorphic site on chromosome 6 of the pig reference genome Sscrofa11.1, specifically the 160th site of the nucleotide sequence shown in SEQ ID NO.1, and the Y of the site is C or T, with C / T polymorphism. The present invention also provides a method for screening pigs with excellent water-power, which can screen pigs at an early stage, effectively solving the problem that muscle drip loss in actual production cannot be screened by live measurement, reducing breeding costs, and the method has high accuracy, low detection costs, and can realize automated detection, and has high practical application value in pig breeding.

This invention provides a method for detecting AAV antibody titers, belonging to the field of biomedical technology, comprising: S1 preparing serum-free AAV vectors with different MOI values; preparing a mixture containing different volumes of test serum samples and AAV vectors with known MOI values ​​for co-incubation; S2: adding plasmids containing the CRISPR-Cas9 gene editing system to electroporation buffer, performing electroporation on a known number of cells, adding the serum-free AAV vectors and mixture from step S1 to the electroporated cells respectively, and detecting the expression efficiency of the reporter gene after electroporation; S3: calculating the AAV antibody titer in the test serum sample based on the detection results. The main advantage of this invention is that it can display AAV antibody titers digitally and quantitatively, facilitating understanding and communication, and obtaining stable and reliable results using different cell lines and different AAV transduction doses. This method is easy to standardize and validate, saving time, effort, and detection costs compared to traditional methods, and directly reflects antibody neutralizing activity.

The application discloses a method for accurately analyzing high-temperature change of niobium compound in steel, which comprises the following steps: (1) fixing a metallographic sample on a clamp; (2) polishing and cleaning the surface of the metallographic sample; (3) observing the metallographic sample by using a metallographic microscope, finding characteristic niobium compound, marking the outer edge of the characteristic niobium compound by using a microhardness tester, and then accurately measuring the size of the marked niobium compound under a scanning electron microscope and accurately determining the composition of the marked niobium compound by using an energy spectrometer; (4) heat treating the metallographic sample to obtain a heat-treated sample; (5) removing the surface oxide of the heat-treated sample and then corroding the surface of the heat-treated sample; and (6) observing the change of the marked niobium compound after heat treatment under a scanning electron microscope and determining the composition of the marked niobium compound by using an energy spectrometer. The method has the characteristics of low detection cost, rapidness, simplicity, accuracy and the like.

The present invention discloses a brain fluctuation graph that supports multi-channel detection, including a brain fluctuation graph host and multiple USB EEG collectors and / or wireless EEG collectors. The UI interface in the host, the USB EEG collector, and the wireless EEG collector are respectively run in separate threads; the separate threads in the USB EEG collector and the wireless EEG collector are used for EEG data reception and decoding and filtering functions, and the decoded and filtered EEG data are exchanged with the separate thread in the UI interface; the USB EEG collector and the wireless EEG collector are both provided with a user information identification module, and the user information identification module is used to receive and display the identity information of the user to be tested sent from the computer host. The brain fluctuation graph that supports multi-channel detection provided by the present invention supports simultaneous detection of multiple people, can improve detection efficiency, and reduce detection costs.

The application belongs to the technical field of chemical analysis, and particularly relates to a detection method for D-glycophosphocholine content in L-alpha-glycophosphocholine and application. The detection method selects a silica gel surface polysaccharide covalent bonding cellulose type or silica gel surface polysaccharide coating cellulose type normal phase chromatographic column and an evaporative light scattering detector (ELSD), detects through an HPLC-ELSD method, has the advantages of simple operation, high sensitivity, good method specificity and reproducibility, is a high-efficiency liquid phase analysis method convenient for standardized operation, can completely separate L-alpha-glycophosphocholine from low-limit D-glycophosphocholine isomer impurities, and can accurately quantify.

The invention relates to a kit for screening dilated cardiomyopathy. The kit comprises a reagent for detecting one or more of genes of TP53, TNF, IL1beta, JUN, CD8A, TLR4, UBB, CTLA4, CXCL8 and NFKBIA. The kit provided by the invention is high in detection efficiency and low in detection cost, provides important reference basis for diagnosis and prognosis judgment of patients with dilated cardiomyopathy, and can remarkably improve the survival rate of the patients.

The utility model provides a conveying device for filter stick sampling, which comprises a collecting cylinder and a cover plate, the cover plate is connected with the end face of the upper end of the collecting cylinder, and the cover plate is used for sealing and covering the upper port of the collecting cylinder; at least two filter stick inlet pipes are arranged on the cover plate; each filter stick inlet pipe is connected with a filter stick conveying pipeline of each filter stick sampling device; the cover plate is further provided with an air inlet pipe used for being connected with an air supply system. The collecting barrel is of a detachable split structure, and a lower end opening of the collecting barrel is a filter stick outlet. According to the utility model, the same comprehensive test platform can be simultaneously butted with filter stick sampling devices of at least two forming machines, so that the number of the comprehensive test platforms can be effectively reduced, the utilization rate of the comprehensive test platforms is improved, and the detection cost of filter sticks is reduced.

The invention discloses a bridge cable capable of rapidly judging the damage condition after a fire and a manufacturing method, the cable comprises a basic element (1) of the bridge cable and at least one low-melting-point alloy wire (2) serving as a high-temperature indicator, in a main cable of a suspension bridge, the basic element (1) comprises a high-strength steel wire strand (11), and the high-strength steel wire strand (11) is connected with the high-strength steel wire strand (11). S-shaped or circular wrapping wires (12) are arranged on the outer sides of the high-strength steel wire strands (11), and a main cable outer wrapping protective layer (13) is arranged on the outer sides of the wrapping wires (12); in a sling and a stay cable, the basic element (1) comprises a semi-parallel high-strength steel wire bundle (14), and a polyethylene sheath (15) is arranged on the outer side of the semi-parallel high-strength steel wire bundle (14). According to the bridge cable, the low-melting-point alloy wire (2) with the appropriate melting point is integrated into a fire-prone area of the cable, the cable slightly affected by a disaster can be rapidly removed through the appearance of the low-melting-point alloy wire (2), and the cable is not prone to fire disaster; the post-disaster detection efficiency is improved, and the post-fire bridge safety assessment time is greatly shortened.

The invention provides corrosive liquid for detecting the proportion of a skip plating area of a copper-plated welding wire and a detection method of the corrosive liquid. The corrosive liquid comprises a hydrochloric acid solution and a lead nitrate solution; the hydrochloric acid solution is prepared by mixing 50-80 ml of analytically pure concentrated hydrochloric acid and 50 ml of water, and the lead nitrate solution is prepared from 5-10 g of analytically pure lead nitrate and 100 g of water; the detection method comprises the steps of sample preparation, chemical corrosion and metallographic microscope observation and analysis. The specific operation comprises the steps that a sample is soaked in the hydrochloric acid solution for 15-30 s, the sample is soaked in the lead nitrate solution for 10-20 s after being washed and blow-dried through alcohol, and after being washed and blow-dried through alcohol, the proportion of the skip plating area is calculated through analysis software of a metallographic microscope according to different proportions of organizational structures. The corrosive liquid and the detection method provided by the invention can completely and clearly display the skip plating area, can quantitatively evaluate the skip plating quality of welding wire copper plating, and have the characteristics of low detection cost, rapidness and simplicity.

A variation site combination related to salt tolerance of wheat, and detection products and applications thereof, belong to the field of molecular genetic breeding technology, the variation site combination is composed of 94 SNP sites and 1 Indel site, the physical position of each variation site is determined based on sequence alignment of wheat reference genome IWGSC RefSeq v.2.1, the Indel is located in the region of 540783807 to 540783852 of 5D chromosome, and is marked as 5D-Indel. The application also provides a nucleotide probe combination and a liquid gene chip developed based on the variation site combination. By using the above variation site combination and detection products, the salt tolerance related genotype of wheat germplasm resources and hybrid offspring can be rapidly screened in early breeding, the accurate identification and directional polymerization of salt tolerance excellent allelic variation can be realized, the dependence on field saline phenotype identification is reduced, the breeding cycle is shortened, and the detection cost and selection efficiency are considered.

The present invention discloses an evaluation device and method for floor impact sound pressure, the evaluation device comprises a test unit and an impact unit which are separately arranged; the test unit comprises a shell and a first bracket for supporting the shell, the shell is hinged on the first bracket through a horizontal X hinge axis and a longitudinal Y hinge axis, a sound collecting hole is arranged on the shell, a diaphragm facing the sound collecting hole and an evaluation system electrically connected to the diaphragm are arranged in the shell; the impact unit comprises an impact object body and an impact object placement part, the impact object body can fall from the impact object placement part and impact the object to be tested. The present invention is suitable for real-time detection at the engineering site, does not need to prepare samples separately, can be quickly moved and arranged between different selected points at the test site, not only effectively meets the real-time detection needs of construction units, design units, construction units, supervision units, owner units, etc. in green building projects, but also can save a lot of test time costs, quickly feedback engineering effects, and greatly reduce detection costs.

This invention discloses an improved method for integrating electrocardiogram (ECG) and electroencephalogram (EEG) into one. Based on the existing ECG DB15 wiring sequence multiplexing circuit, it fully utilizes the reserved empty pins (NC pins) in the wiring sequence without changing the core definition of the original DB15 wiring sequence or affecting the original 12-lead ECG signal acquisition function. This achieves integrated reuse of the ECG / EEG interface and circuit, eliminating the need for an additional independent EEG interface and complex circuitry. Furthermore, this invention, through a built-in recognition module, can automatically identify whether the user has inserted an ECG or EEG cable and quickly switch the corresponding signal acquisition parameters, filtering modes, and data processing logic. This enables automatic switching between ECG and EEG acquisition functions without manual adjustment, making operation convenient and efficient. It effectively reduces the operational difficulty for medical personnel and minimizes detection errors caused by human error.

The invention discloses a cigar draw resistance measuring method and system and a storage medium, and belongs to the cross technical field of computational fluid mechanics and tobacco analysis technology, the draw resistance measuring method comprises the following steps: obtaining a cigar transient aerodynamic system control equation; suction experiments are carried out in the cigar sealing state and the cigar unsealing state respectively, and pressure data under different suction flows are obtained; substituting into a cigar transient aerodynamic system control equation to obtain a corresponding relation between transient pressure and transient flow; obtaining a mapping relation between the transient pressure of the cigar in the sealed state and the transient pressure of the cigar in the unsealed state; the transient actual measurement pressure in the sealed state of the cigar cigarette is obtained, the transient actual measurement pressure in the unsealed state of the cigar cigarette is obtained, and the transient actual measurement pressure in the unsealed state of the cigar cigarette is the suction resistance of the cigar cigarette. According to the invention, nondestructive detection can be carried out on cigars, detection samples can be enlarged, and the accuracy of draw resistance results is improved.

The invention provides a prostate cancer detection method and kit based on exosome RNA, and the method comprises the following steps: preparing nano-scale cationic lipid particles coated with RNA molecular beacons, each RNA molecular beacon is combined with a corresponding target exosome to generate fluorescence, and the fluorescence is used for detecting the prostate cancer. The target exosome is allowed to be one or more of the following exosomes in the urine: miR-141, miR-375, PCA3 and T1-E2; a porous chip plate is coated with an exosome capture system and nanoscale cationic lipid particles to form the detection kit; the exosome capturing system is used for capturing exosomes; a to-be-detected sample is pretreated, the pretreated to-be-detected sample is added into the kit, and whether a subject suffers from the prostate cancer or not is judged according to a fluorescence imaging result of the kit; wherein the to-be-detected sample at least comprises urine of a subject, non-invasive detection of prostatic cancer can be realized, the detection cost is low, the time is short, and the cost is relatively low.

The application discloses a method and system for assisting in determining the grade of brain glioma, and belongs to the technical field of biological genes and bioinformatics. The method comprises the following steps: obtaining sample information of a patient and pre-processing the sample; performing FISH detection, NGS detection assisted bioinformatics analysis and pathological detection on the sample to obtain N1 FISH detection indexes, N2 NGS detection indexes and N3 pathological detection indexes, and gene variation phenomenon characteristics corresponding to each detection index; and determining the type and grade of brain glioma based on the detection indexes and the gene variation phenomenon characteristics corresponding to one or more of the detection indexes. The application also discloses corresponding systems, electronic devices and computer readable storage media. The NGS sequencing technology is used to replace part of the conventional FISH detection mode, the NGS data is retained, secondary analysis is performed by using the bioinformatics method to obtain new molecular indexes in the guidelines, the detection types and times are reduced, and the detection mode is optimized.

The present invention relates to the field of data processing technology, and specifically to a method for recommending a diagnosis and treatment plan based on patient data analysis. The method for recommending a diagnosis and treatment plan based on patient data analysis includes: obtaining the current patient's physical state data. Analyze the patient's current physical state data, and calculate the symptom bias coefficients of each symptom of the patient. Determine whether the current patient's maximum symptom bias coefficient is greater than or equal to a preset standard coefficient. If so, determine that the symptom corresponding to the symptom bias coefficient is the symptom suffered by the patient, and then find a pre-set symptom-treatment plan information table according to the symptom to obtain the treatment plan for the symptom. If not, recommend the inspection of the test items that are required and not detected for the symptom corresponding to the symptom bias coefficient. This method can automatically diagnose and analyze the patient, and determine whether the diagnosis plan can meet the judgment conditions. The recommendation level is good and can be more targeted at the actual situation of the patient.

The invention relates to a model and method for evaluating general disease resistance of tilapia mossambica and application, and belongs to the technical field of aquaculture. The model for evaluating general disease resistance of tilapia mossambica detects eight immune indexes including serum immune indexes (SOD, ACP, AKP, C3, TNF-alpha, IFN-gamma and IgM) and mucus immune indexes (IgT) of tilapia mossambica, then nondimensionalizes the detection values of the immune indexes into Z scores, and finally evaluates the disease resistance of tilapia mossambica according to the Z scores. The disease resistance index fi of each individual or group is calculated and obtained by substituting the obtained disease resistance index f into the established comprehensive disease resistance expression of the tilapia mossambica, the larger the f value is, the stronger the disease resistance is, the smaller the f value is, the weaker the disease resistance is, and families and individuals in the disease resistance breeding process of the tilapia mossambica can be selected or eliminated according to the f value. The method well solves the problems that tilapia disease resistance characters cannot be quantitatively analyzed, and weights of different immune indexes in comprehensive evaluation of tilapia disease resistance are inconsistent. The detection method disclosed by the invention is simple to operate and high in accuracy, and can be applied to disease-resistant breeding work of tilapia mossambica.

The curbstone-free asphalt pavement construction method comprises the following steps: constructing a roadbed at a design position of an asphalt pavement, and enabling the top surface of the roadbed to be flush with the top surfaces of green belt planting soil on two sides; baffles are connected to the two sides, relatively close to green belt planting soil on the two sides, of the top of the roadbed, the baffles are arranged in the length direction of the roadbed in a full-length mode, and the top elevation of the baffles is flush with the top design elevation of the asphalt pavement; coarse aggregate asphalt is poured on the inner sides of the two baffles until reaching the designed position, soil is backfilled on the outer sides of the two baffles for the first time, and the soil body top elevation of the soil backfilled for the first time is flush with the top elevation of the coarse aggregate asphalt; fine aggregate asphalt is poured on the inner sides of the two baffles till the fine aggregate asphalt is flush with the top faces of the baffles, soil is backfilled on the outer sides of the two baffles for the second time, the elevation of the top of a soil body of the backfilled soil for the second time is flush with the top faces of the baffles, and through the construction method, the cost is saved, and the construction efficiency is improved.

The present invention belongs to the field of biomedicine technology, and discloses a marker for early screening of cardiac adenocarcinoma in high-risk populations, the marker being GAGE12B protein or a combination of GAGE12B protein and AGPAT4 protein or a combination of GAGE12B protein and P2RX4 protein, and by detecting the expression level of the above marker in serum, cardiac adenocarcinoma can be effectively detected. The present invention also discloses a test strip for early screening of cardiac adenocarcinoma in high-risk populations, the test strip binding pad is coated with a capture antibody, the capture antibody is a mixture of GAGE12B antibody A and AGPAT4 antibody A; two detection lines and a quality control line are provided on the chromatography pad, and detection antibodies are fixed on the detection lines, and the detection antibodies fixed on the two detection lines are GAGE12B antibody B and AGPAT4 antibody B respectively; rabbit anti-mouse IgG is fixed on the quality control line. The test strip of the present invention can quickly detect GAGE12B and AGPAT4 in human serum, and can effectively detect cardiac adenocarcinoma according to the detection results, greatly improving the detection rate of early cardiac adenocarcinoma.

The invention relates to the technical field of operation guidance, and discloses an eyeball three-dimensional model construction method and a macular buckle operation navigation system.The method comprises the following steps that S1, data extraction of all parts of an eyeball is conducted, specifically, S11, cornea parameter establishment and variability analysis are conducted; s12, establishing parameters of the front half part of the eyeball and carrying out variability analysis; s13, parameter establishment and variability analysis of the rear half part of the eyeball; s2, constructing an eyeball three-dimensional model through the cornea parameters, the eyeball front half part parameters and the eyeball rear half part parameters. The system is matched with the method. According to the method, the difficulty of eyeball three-dimensional reconstruction is effectively reduced, the macular positioning precision is greatly improved, the operation time is shortened, the difficulty of a macular buckle operation is reduced, and individualized accurate implementation of the macular buckle operation is realized.