7results about How to "Low detection cost" patented technology

This invention provides a method for detecting AAV antibody titers, belonging to the field of biomedical technology, comprising: S1 preparing serum-free AAV vectors with different MOI values; preparing a mixture containing different volumes of test serum samples and AAV vectors with known MOI values ​​for co-incubation; S2: adding plasmids containing the CRISPR-Cas9 gene editing system to electroporation buffer, performing electroporation on a known number of cells, adding the serum-free AAV vectors and mixture from step S1 to the electroporated cells respectively, and detecting the expression efficiency of the reporter gene after electroporation; S3: calculating the AAV antibody titer in the test serum sample based on the detection results. The main advantage of this invention is that it can display AAV antibody titers digitally and quantitatively, facilitating understanding and communication, and obtaining stable and reliable results using different cell lines and different AAV transduction doses. This method is easy to standardize and validate, saving time, effort, and detection costs compared to traditional methods, and directly reflects antibody neutralizing activity.

The application discloses a method for accurately analyzing high-temperature change of niobium compound in steel, which comprises the following steps: (1) fixing a metallographic sample on a clamp; (2) polishing and cleaning the surface of the metallographic sample; (3) observing the metallographic sample by using a metallographic microscope, finding characteristic niobium compound, marking the outer edge of the characteristic niobium compound by using a microhardness tester, and then accurately measuring the size of the marked niobium compound under a scanning electron microscope and accurately determining the composition of the marked niobium compound by using an energy spectrometer; (4) heat treating the metallographic sample to obtain a heat-treated sample; (5) removing the surface oxide of the heat-treated sample and then corroding the surface of the heat-treated sample; and (6) observing the change of the marked niobium compound after heat treatment under a scanning electron microscope and determining the composition of the marked niobium compound by using an energy spectrometer. The method has the characteristics of low detection cost, rapidness, simplicity, accuracy and the like.

The application belongs to the technical field of chemical analysis, and particularly relates to a detection method for D-glycophosphocholine content in L-alpha-glycophosphocholine and application. The detection method selects a silica gel surface polysaccharide covalent bonding cellulose type or silica gel surface polysaccharide coating cellulose type normal phase chromatographic column and an evaporative light scattering detector (ELSD), detects through an HPLC-ELSD method, has the advantages of simple operation, high sensitivity, good method specificity and reproducibility, is a high-efficiency liquid phase analysis method convenient for standardized operation, can completely separate L-alpha-glycophosphocholine from low-limit D-glycophosphocholine isomer impurities, and can accurately quantify.

The invention relates to a kit for screening dilated cardiomyopathy. The kit comprises a reagent for detecting one or more of genes of TP53, TNF, IL1beta, JUN, CD8A, TLR4, UBB, CTLA4, CXCL8 and NFKBIA. The kit provided by the invention is high in detection efficiency and low in detection cost, provides important reference basis for diagnosis and prognosis judgment of patients with dilated cardiomyopathy, and can remarkably improve the survival rate of the patients.

A variation site combination related to salt tolerance of wheat, and detection products and applications thereof, belong to the field of molecular genetic breeding technology, the variation site combination is composed of 94 SNP sites and 1 Indel site, the physical position of each variation site is determined based on sequence alignment of wheat reference genome IWGSC RefSeq v.2.1, the Indel is located in the region of 540783807 to 540783852 of 5D chromosome, and is marked as 5D-Indel. The application also provides a nucleotide probe combination and a liquid gene chip developed based on the variation site combination. By using the above variation site combination and detection products, the salt tolerance related genotype of wheat germplasm resources and hybrid offspring can be rapidly screened in early breeding, the accurate identification and directional polymerization of salt tolerance excellent allelic variation can be realized, the dependence on field saline phenotype identification is reduced, the breeding cycle is shortened, and the detection cost and selection efficiency are considered.

This invention discloses an improved method for integrating electrocardiogram (ECG) and electroencephalogram (EEG) into one. Based on the existing ECG DB15 wiring sequence multiplexing circuit, it fully utilizes the reserved empty pins (NC pins) in the wiring sequence without changing the core definition of the original DB15 wiring sequence or affecting the original 12-lead ECG signal acquisition function. This achieves integrated reuse of the ECG / EEG interface and circuit, eliminating the need for an additional independent EEG interface and complex circuitry. Furthermore, this invention, through a built-in recognition module, can automatically identify whether the user has inserted an ECG or EEG cable and quickly switch the corresponding signal acquisition parameters, filtering modes, and data processing logic. This enables automatic switching between ECG and EEG acquisition functions without manual adjustment, making operation convenient and efficient. It effectively reduces the operational difficulty for medical personnel and minimizes detection errors caused by human error.

The application discloses a method and system for assisting in determining the grade of brain glioma, and belongs to the technical field of biological genes and bioinformatics. The method comprises the following steps: obtaining sample information of a patient and pre-processing the sample; performing FISH detection, NGS detection assisted bioinformatics analysis and pathological detection on the sample to obtain N1 FISH detection indexes, N2 NGS detection indexes and N3 pathological detection indexes, and gene variation phenomenon characteristics corresponding to each detection index; and determining the type and grade of brain glioma based on the detection indexes and the gene variation phenomenon characteristics corresponding to one or more of the detection indexes. The application also discloses corresponding systems, electronic devices and computer readable storage media. The NGS sequencing technology is used to replace part of the conventional FISH detection mode, the NGS data is retained, secondary analysis is performed by using the bioinformatics method to obtain new molecular indexes in the guidelines, the detection types and times are reduced, and the detection mode is optimized.