The present application relates to the technical field of flow monitoring, and discloses a method and
system for real-time monitoring of flow in an At-211 column
separation process, the method comprising: collecting the
conductivity of eluent in the outlet pipeline of the At-211
chromatographic column in real time, continuously analyzing the
conductivity, and generating a state flag; wherein when it is detected that the eluent is switched to
anhydrous ethanol phase, the state flag is valid; otherwise, the state flag is invalid; by collecting the
conductivity and generating the state flag, the timing of
anhydrous ethanol phase switching is accurately determined, the collected pressure
time series data is analyzed to generate a steady-state pressure value, then
secondary analysis is performed to obtain a pressure memory value, the
stable state of pressure and the continuous influence of flow are comprehensively captured, and finally a flow
stability index is generated, which can evaluate the flow state of the whole
alcohol phase
elution process. The present application does not need to rely on the continuity of the conductivity
signal, effectively filters transient interference, and improves the accuracy of flow monitoring.