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185 results about "Primary screening" patented technology

Primary screening comprises of certain elementary tests needed to detect and to isolate new microbial species exhibiting the preferred property. With antibiotic producers, primary screening programs serve to eliminate worthless micro-organisms on the basis of relatively simple, basic criteria.

Early cancer screening model based on cloud architecture and construction method

The invention discloses an early cancer screening model based on a cloud architecture and a construction method, and particularly relates to the technical field of medical artificial intelligence. The method comprises the following steps: firstly, collecting and normalizing heterogeneous medical image and biomarker data of a plurality of geographic areas, and constructing a unified data set; then extracting image features and marker features by adopting multi-task learning, fusing equipment and region attributes, establishing a joint representation vector, and training a preliminary screening model; after the model is deployed to a cloud end, target area data is adapted through online transfer learning, and an area model is generated; further extracting an activation heat map, identifying a weak signal high response area in combination with sensitivity analysis, comparing with manual annotation, constructing a local weight adjustment strategy based on errors, optimizing the model, and outputting a final model; the method improves the recognition capability of early lesions under the condition of low signal-to-noise ratio, is suitable for remote and basic screening scenes, and has a high practical value.
Owner:CHENGDU SIKU INTELLIGENT TECH CO LTD

Computer-implemented method and system for non-invasive and step-by-step prediction of cancer risk, and storage medium

The invention provides a computer-implemented method and system for non-invasive and step-by-step prediction of cancer risks and a storage medium. The method comprises the following steps: (1) determining a cancer signal score based on the level of a biomarker in a blood sample of a subject; (2) comparing the cancer signal score with a preset threshold value, and determining a primary screening positive subject; (3) further performing cancer risk determination and cancer species prediction by adopting an NGS method on the basis of the primarily screened positive subjects; wherein the biomarker is selected from at least one of AFP (Alpha Fetal Protein), CA125, CA15-3, CA19-9, CA72-4, CEA (Carcino Embryonic Acid), CYFRA (Cytochrome Factor Receptor Amplification) 21-1, ProGRP (Proso-Growth Radical Polymerase Chain Reaction), SCCA (Sequence
Owner:SEEKIN INC SHENZHEN CHINA

Large-specification red paste macrobrachium rosenbergii cultivation method

The present application belongs to the field of aquaculture, and particularly relates to a large-specification red paste Macrobrachium rosenbergii breeding method, which comprises the following steps: S1, seedling marking: seedlings are raised in a greenhouse, a pond, an adjustable feed throwing device and a networked automatic feed checking table are arranged in the greenhouse; the feed feeding amount of the adjustable feed throwing device is adjusted through feedback of the networked automatic feed checking table; water quality is adjusted by using micro-ecological preparation and bottom modification preparation; S2, initial screening of male and female: when the marking time is 45-50 days and the average specification reaches 1800-2000 tails / kg, initial screening of male and female is performed to reduce the density of the Macrobrachium rosenbergii in the greenhouse; S3, outdoor pond raising; S4, red paste fattening: female Macrobrachium rosenbergii are fattened with red paste, nutrition fattening feed is fed on Monday, Wednesday and Friday every week, snail meat, carrots and corn are used to replace part of the feed in turn, the replacement proportion is about 50%-60%, normal feed is fed at other times, the fattening period is 4-6 weeks, and the Macrobrachium rosenbergii are caught in late September to early October.
Owner:ZHEJIANG INST OF FRESH WATER FISHERIES +1

Plateau newborn inherited metabolic disease screening system, computer readable storage medium and application of plateau newborn inherited metabolic disease screening system

The invention belongs to the technical field of medical software development, and particularly relates to a plateau newborn inherited metabolic disease screening system, a computer readable storage medium and application of the plateau newborn inherited metabolic disease screening system. According to the screening system, an altitude hierarchical multi-level reference value database based on a local healthy newborn queue is established, and the problem that the false positive rate of a plain area screening model in a plateau area is too high is solved by fitting an altitude metabolism marker reference median and a standard deviation curve. By designing a'biochemical primary screening-risk scoring-targeted sequencing automatic triggering 'three-level joint control data processing flow, targeted sequencing guided by a crowd specific pathogenic mutation library is started only when the biochemical risk is high, unnecessary whole genome sequencing is avoided, and the screening cost is remarkably reduced. The system can continuously iterate a reference curve and a risk threshold value according to definite diagnosis data, and closed-loop dynamic optimization from sample collection to report generation is achieved. The method has plateau adaptability, crowd specificity and cost controllability, and has a good clinical application prospect.
Owner:THE WEST CHINA SECOND UNIV HOSPITAL OF SICHUAN +1

A method for genetic transformation and gene editing applicable to multiple species of achenes

This invention discloses a genetic transformation and gene editing method applicable to multiple sesquiterpene species, belonging to the field of plant genetic transformation and gene editing technology. The method uses the cotyledonary nodes of sterile seedlings of the sesquiterpene genus as explants, and introduces an optimized pScEF1α-Cas9 gene editing transformation vector using Agrobacterium infection. Regenerated plants are obtained through recovery culture, selection culture, and rooting culture. The vector contains four gene expression units: pScEF1α:Cas9, pGmU6:sgRNA, pGmUBI:DsRed2, and pCaMV35S:HygR. In vivo screening is further performed using the DsRed2 fluorescent reporter gene, and the gene-edited plants are identified by molecular detection. This method is applicable to common sesquiterpene, stem-nodled sesquiterpene, and spiny sesquiterpene, and has advantages such as strong species versatility, high transformation efficiency, short cycle, visualized screening, and high positive rate, providing efficient technical support for gene function research and genetic improvement of sesquiterpene species.
Owner:INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI

Intelligent food sorting and sand removal equipment based on photoelectric sensing

This utility model discloses a sand and gravel removal device for intelligent sorting of food ingredients based on photoelectric sensing. It includes a belt conveyor, a dispersing mechanism, a primary screening mechanism, and a control mainboard. A column is fixedly installed on one side of the belt conveyor, and a connecting rod one is fixedly installed on the outer wall of the middle part of the column. A connecting rod two is fixedly installed on one side of the upper end of the column. The primary screening mechanism is fixedly installed on one side of the column, directly above the belt conveyor. The dispersing mechanism is fixedly installed on one side of the connecting rod two. The primary screening mechanism filters out fine sand and gravel, thus reducing the detection range of the device. The remaining food ingredients and large sand and gravel mixture are sent to the sorting cylinder. Because the opening of the sorting cylinder is small, only a small amount of food ingredients and sand and gravel can pass through at a time, further reducing the detection range of the device. By performing preliminary screening of food ingredients and sand and gravel while controlling the detection inlet, the scope of the device's inspection is reduced, thereby reducing the required precision and cost of the device.
Owner:广东润康药业有限公司

A gradient temperature difference processing method for controlling rancidity deterioration of rice bran decoction pieces

ActiveCN122005722BResistance and control breedResist pollutionBiotechnologyMicroorganism
The application provides a gradient temperature difference processing method for preventing and controlling rancidity and deterioration of rice bran decoction pieces, comprising the following steps: sequentially subjecting newly harvested rice bran to low-temperature rapid cooling, medium-temperature roasting, high-temperature roasting and second ultra-low-temperature rapid cooling after removing impurities by primary screening, so as to prevent and control rancidity and deterioration of rice bran decoction pieces. The application utilizes rapid and strong temperature difference change to prevent and control the reaction after grinding contact oxidation, destroys the autoxidation system of rice bran, effectively prevents and controls the generation of oxidized toxic substances and the breeding and pollution of harmful microorganisms, and ensures the safety and effectiveness of medication.
Owner:HUNAN MIZHENBAO BIOLOGICAL HIGH TECH CO LTD

Beta-glucosidase mutant as well as preparation method and application thereof

PendingCN121950986ABacteriaMicroorganism based processesBacillus thuringiensisEngineered genetic
The invention provides a beta-glucosidase mutant from Paenibacillus phyllosphaerae, and a method for efficiently preparing rare ginsenoside F2 by using an enzyme method and a whole-cell method, and particularly provides a beta-glucosidase mutant from Paenibacillus phyllosphaerae and a method for efficiently preparing rare ginsenoside F2 by using a beta-glucosidase mutant from Paenibacillus phyllosphaerae. The method comprises the following steps: engineering-modifying beta-glucosidase by utilizing computer-aided design, constructing a mutant library, carrying out primary screening and secondary screening to obtain two high-activity mutants Q7D and G189A, constructing a combined mutant Q7D / G189A, transforming the combined mutant Q7D / G189A into genetically engineered bacterium escherichia coli for expression, and producing F2 by utilizing a whole-cell method. Finally, the highest yield of 18.01 g / L is achieved after the reaction is carried out for 10 h in a 5 L fermentation tank and is 6.82 times that of a shake flask, and the purity of F2 reaches up to 82.20% after the reaction is carried out for 24 h finally. The invention not only provides an efficient solution for large-scale production of rare saponin F2, but also provides a transferable framework for designing and engineering diversified glycoside hydrolase in customized biological catalysis.
Owner:SOUTHWEST UNIV

Breeding method and system based on multi-dimensional data

The embodiment of the present application relates to the technical field of breeding management, and discloses a selection and breeding method based on multidimensional data, which comprises the following steps: determining the feather characteristic parameters of chicks according to feather characteristic information and pixel point size; determining the gender and predicting the growth parameter information of the corresponding chicks according to the length of the primary wing feather and the length of the over primary wing feather, and performing primary screening according to the predicted growth parameter information; inputting the image information of the breeding hen into a feature recognition model to obtain the corresponding characteristics of the breeding hen; inputting the comb image into a comb recognition model to determine the corresponding comb type; if the feather color, the chicken toe color and the comb type match the breed parameters of the corresponding breeding hen, then the breeding hen is kept and caged, and the individual characteristic parameters are recorded. The scheme of the embodiment of the present application realizes the deep fusion of image recognition technology and multidimensional phenotype data, constructs a full-cycle accurate selection and breeding system from chicks to breeding hens, and realizes the automation of gender identification, the standardization of breed verification and the intelligentization of growth prediction.
Owner:JIANGMEN KELANG AGRI TECH CO LTD +1

A method for rapid screening of high-yield triglyceride-type polyunsaturated fatty acid filamentous fungi

This invention relates to a method for rapidly screening filamentous fungi that produce high levels of triglycerides and polyunsaturated fatty acids, belonging to the field of microbial technology. The method employs a two-stage screening strategy of "resistance primary screeningfluorescence secondary screening." In the primary screening stage, strains with strong lipid synthesis capabilities are initially screened by adding a combination of phytocyanin and quizalofop-p-ethyl inhibitors to the culture medium. In the secondary screening stage, cellophane culture technology and Nile red fluorescence staining are combined to quantitatively assess the triglyceride content of the colonies, achieving rapid and non-destructive screening. This method is simple to operate, highly efficient, and shows a significant positive correlation between fluorescence intensity and triglyceride content. It is suitable for mutagenesis breeding and industrial strain selection of oil-producing filamentous fungi such as *Morchella alpineensis*.
Owner:WUXI INSTITUTE FOR SPECIALIZED NUTRITION & HEALTH CO LTD

A screening device for heavy calcium carbonate particles

The application discloses a screening device for heavy calcium carbonate particles, and relates to the technical field of screening, which comprises a frame body, an outer cover installed on the frame body, a support frame fixedly installed in the outer cover, a double-screen assembly rotatably installed on the support frame, and a primary screen assembly rotatably installed in the double-screen assembly, and a tumbling assembly matched with the primary screen assembly is installed on the support frame. Advantages are as follows: the heavy calcium carbonate particle screening device realizes two-stage screening through reverse rotation of double rollers, utilizes the staggered effect to diffuse materials and improves the efficiency; a driving motor is provided to drive a filter plate to scatter the accumulated materials of the inner roller, so that the diffusion of the materials is accelerated; a gas injection rotating pipe with fins is used to further diffuse the materials through pulse blowing, and dust is sucked and discharged at the same time; the rotating pipe is controlled to rotate at variable speed through gas injection pressure, and an elastic expansion rod cooperates with a counterweight ball to knock and vibrate, so that the rotating pipe is prevented from being blocked.
Owner:ZHANGJIAJIE HENGLIANG MINING

Redirection breeding method for tussah tussah tall thousand-grain cocoons

The invention provides a tussah tussah tall thousand-grain cocoon redirection breeding method which comprises the following steps: partitioning a breeding basic group of tussah, and stocking in a plurality of moth regions under the same ecological condition; after cocooning, measuring the weight of thousand cocoons by taking the moth area as a unit, and screening out a target moth area based on a measurement result; performing sex identification and whole cocoon quantity measurement on the silkworm cocoons in the target moth area, and screening out a target female individual set and a target male individual set according to data of the whole cocoon quantity; regulating and controlling the eclosion time of individuals in the target female individual set and the target male individual set to synchronize the eclosion time, and performing directional mating between the two sets according to a preset pairing rule based on the data of the total cocoon amount; and taking the filial generation obtained by directional mating as a breeding base population of the next generation, and carrying out continuous multi-generation directional breeding until a tussah silkworm strain with stable thousand-grain cocoon weight character is obtained. According to the method, a breeding path of primary screening, fine screening, directional mating and multi-generation closed loop is constructed, so that breakthrough increase of thousand-grain cocoon weight is realized.
Owner:SERICULTURE RES INST OF LIAONING PROVINCE

Glass bead feeding device and method for radioactive waste liquid glass solidification

The invention discloses a glass bead feeding device and method for radioactive waste liquid glass solidification, the glass bead feeding device comprises a drying primary screening device, a fine screening device, a storage bin and a discharging device which are sequentially arranged according to the flowing sequence of glass beads, the drying primary screening device comprises a baking box, and a heater is arranged at the top in the baking box; a feeding hopper and a discharging hopper are arranged on the two sides of the baking box respectively, the fine screening device is arranged below the discharging hopper, a primary screening mechanism located between the feeding hopper and the discharging hopper is arranged in the baking box, and the primary screening mechanism is used for primarily screening out glass beads meeting the particle size and conveying the glass beads to the discharging hopper. The screening device has the advantages that the follow-up screening pressure is reduced, the number of times of cleaning the screen is reduced, and the working efficiency and the screening precision are improved.
Owner:SICHUAN ENVIRONMENTAL PROTECTION ENG CO LTD CNNC

A non-destructive insect feed separation device

This application provides a non-destructive separation device for insect feed, relating to the field of separation equipment technology. The device includes a main frame and a primary screening unit mounted on the main frame, with a secondary screening unit located below the primary screening unit. The primary screening unit includes a primary screening box slidably connected to the main frame and a first driving component for driving the primary screening box to reciprocate. The sliding direction of the primary screening box on the main frame is inclined relative to the horizontal plane. The bottom of the primary screening box is a horizontally arranged arc-shaped portion, and a screening perforated plate is provided at the bottom of the primary screening box. The secondary screening unit includes a secondary screening box, within which a screening drawer is provided. One end of the screening drawer has a discharge port, and a lighting lamp is located at the end of the secondary screening box furthest from the discharge port. This device can achieve low-loss, high-purity separation of larvae and insect excrement.
Owner:LINYI UNIVERSITY +1

Wheat haploid accurate identification and breeding method based on molecular marker

The invention discloses a wheat haploid accurate identification and breeding method based on molecular markers, and belongs to the technical field of agricultural biology. The method comprises the following steps: hybridizing a created multifunctional haploid induction line HIP-Plus serving as a male parent with a target female parent, and saving immature embryos to obtain seedlings; a three-stage identification system comprising visual preliminary screening, molecular confirmation and early selection is adopted to carry out accurate screening on seedlings: firstly, preliminary screening is carried out based on embryo color, then haploid confirmation and transgenic component detection are carried out by utilizing TaMTL allele specific markers, and finally genetic typing is carried out through important character function markers to screen out elite haploids; and carrying out chromosome doubling to obtain a double haploid homozygous line. According to the method, the haploid identification accuracy is improved, the breeding period is shortened, the early directional selection of the target agronomic traits in the haploid stage is realized, and the breeding efficiency and accuracy are greatly improved.
Owner:XINJIANG JIUHE SEED IND CO LTD

Method for identifying and adulterating based on multi-gene combined DNA barcoding of sea cucumber base and adulteration

The application provides a fat sea base gene and adulteration identification method based on multi-gene joint DNA barcoding, four gene joint barcode combinations are formed by adopting nuclear gene ITS2 and chloroplast genes matK, rbcL and psbA-trnH, at least two pairs of optimized alternative primers for each target gene and fat sea genus specific preliminary screening primer Ster-1 are matched, genus level rapid preliminary screening is realized to exclude non-fat sea genus samples and shorten the identification process, and the efficient amplification success rate of multi-gene fragments is ensured through the flexible use of alternative primers; fat sea and round fat sea, Sterculia and mixed adulterants can be accurately distinguished, the problems that traditional identification methods are subjective and species with close genetic relationship are difficult to distinguish are effectively solved; the method is not limited by sample morphology and fragmentation degree, can be widely applied to the true and false identification, quality control and traceability of traditional Chinese medicinal materials fat sea and its products, and provides technical support for standardizing market order and ensuring the safety and effectiveness of clinical medication.
Owner:YANGTZE RIVER PHARM GRP CO LTD

A comprehensive decision-making and evaluation method for halogenated hydrocarbon contaminated soil and groundwater remediation strategy

The application provides a comprehensive decision-making and evaluation method for halogenated hydrocarbon contaminated soil and groundwater remediation strategy, comprising: establishing a site baseline and conducting technical preliminary screening based on health and ecological risk assessment (HERA) and a three-level evidence system; conducting rapid resource efficiency assessment based on environmental footprint analysis (EFA); conducting global long-term assessment based on life cycle assessment (LCA) and cost-benefit analysis (CBA); and dynamic evaluation and closed-loop control based on digital twinning and multi-criteria decision analysis (MCDA) coupling. In the dynamic evaluation stage, an adaptive hybrid dynamic weighting mechanism is constructed, the subjective benchmark weight and the objective fluctuation weight calculated based on real-time data information entropy are integrated, and the comprehensive utility score is calculated; at the same time, the apparent degradation rate constant of the pollutant is solved in real time, and when it is lower than the target rate and the strategy is replaced, the intervention instruction is automatically output. The application realizes the deep integration of multi-dimensional indicators, dynamic adaptive evaluation and closed-loop intelligent regulation and control, and solves the problems of single, static and lack of feedback of the existing evaluation method.
Owner:XIAN UNIV OF TECH

Self-cleaning type smelting waste residue collecting device

The invention provides a self-cleaning type smelting waste residue collecting device and relates to the technical field of smelting waste residue treatment.The self-cleaning type smelting waste residue collecting device comprises a screening tank body, a primary screening unit, a secondary screening unit and a self-cleaning unit are arranged in the screening tank body, the lower end of the secondary screening unit is connected with a driving mechanism, and the upper end and the lower end of the screening tank body are provided with a feeding hopper and a discharging opening correspondingly; the primary screening unit and the secondary screening unit are distributed in the screening tank body from top to bottom and communicate with the feeding hopper and the discharging opening, and the self-cleaning unit communicates with the primary screening unit and the secondary screening unit up and down. The device has the beneficial effects that the grading effect is optimized through combination of two-stage screening and different-axis blanking, comprehensive self-cleaning is achieved through a follow-up spraying dual mode, manual disassembly is replaced, the operation stability is improved, the service life of equipment is prolonged, the device is automatically adapted to various smelting waste residues, and the maintenance cost and the operation intensity are reduced.
Owner:安徽帕卓流体科技有限公司

A combined detection test strip for dengue virus, zika virus antigen and antibody and a preparation method thereof

The application provides a combined detection test strip for dengue virus, Zika virus antigen and antibody and a preparation method thereof; the combined detection test strip of the application adopts a multi-site recombinant antigen marking or multi-site recombinant antibody coating form for the combined detection test strip for dengue virus NS1 antigen and dengue virus IgG / IgM antibody, thereby improving the sensitivity of the reagent; the application also overcomes the problems of poor specificity and release residue of the latex microspheres by optimizing a blocking solution; color latex microspheres are used as the markers, the target is more intuitively detected according to the color of the lines, clinical result interpretation is facilitated, the accuracy of dengue virus infection and Zika virus infection is improved, dengue virus NS1, Zika virus NS1 antigen, dengue virus IgG / IgM antibody detection and Zika virus IgG / IgM antibody can be simultaneously detected; moreover, the application is convenient to carry and easy to operate, is convenient for on-site instant detection and can be used for large-scale preliminary screening.
Owner:JIANGSU MOLE BIOSCI +1

A seed screening device

This invention discloses a seed screening device, belonging to the field of seed screening technology. It includes a primary screening chamber containing a first separation chamber, a second separation chamber, and a third separation chamber. These chambers are separated by a first partition. A first discharge pipe is installed at the lower end of the first separation chamber. A secondary screening chamber and a second fixing frame are located on one side of the lower ends of the second and third separation chambers. The secondary screening chamber contains a fourth and a fifth separation chamber, separated by a second partition. The fifth separation chamber includes a waste bin and a recycling bin, separated by a third partition. This invention significantly improves seed screening accuracy and yield through multi-stage screening, dynamic airflow control, and automated sorting, while reducing energy consumption and maintenance costs, making it suitable for large-scale agricultural processing applications.
Owner:张广磊

Coastal saline-alkali land green manure combined desalting and fertilizing method based on multistage screening

The invention belongs to the technical field of saline-alkali soil improvement and agricultural biology, and particularly relates to a coastal saline-alkali soil green manure combined desalting and fertilizing method based on multistage screening. According to the method, sand culture and water culture are combined, different NaCl concentration gradients are set, the salt tolerance of green manure crops is evaluated, and green manure varieties suitable for different salinization degrees are screened out; planting the screened green manure varieties in different areas according to the salinization degree of the saline-alkali soil; after the green manure grows to a proper period, turning and returning to the field to realize soil desalination and fertilization. According to the method, multi-dimensional agronomic characters such as the emergence rate, the root length, the plant height and the fresh weight are comprehensively evaluated through a multi-stage screening method combining sand culture primary screening and water culture secondary screening, green manure varieties suitable for mild, moderate and severe salinized soil are scientifically screened out, the defects that in the prior art, green manure selection is blind, and a targeted evaluation system is lacked are overcome, and the method is suitable for large-scale popularization and application. And accurate variety support is provided for biological improvement of the coastal saline-alkali land.
Owner:INST OF SOIL & FERTILIZER FUJIAN ACADEMY OF AGRI SCI

Rapid breeding method of high-dietary-fiber glutinous highland barley

InactiveCN121753701ABiocideWeighing by removing componentBiotechnologyEarly generation
The invention relates to the technical field of crop breeding, and discloses a rapid breeding method of high-dietary-fiber glutinous highland barley, which comprises the following steps: seed pretreatment, chemical mutagenesis treatment, culture solution preparation, greenhouse culture and early comprehensive screening, rapid genetic homozygosis and high-generation accurate phenotype selection. By preparing mutagenesis liquid with specific components to induce seeds, a chemical mutagenic agent contained in the mutagenesis liquid can initiate directional variation of genetic materials of the highland barley seeds, so that the genetic basis of high dietary fibers and waxy characters is widened, and rich variant groups are provided for subsequent screening; meanwhile, in the greenhouse culture and early comprehensive screening stage, the content of beta-glucan in seeds of a single plant is subjected to nondestructive measurement by utilizing a near infrared spectrum technology, so that high-throughput and low-cost quality primary screening on a huge group in the early generation of breeding is realized, and the bottlenecks that a traditional chemical detection method is long in period, high in cost and cannot be used for early generation selection are overcome; therefore, the screening efficiency of the germplasm resources is improved.
Owner:GANSU RES INST OF AGRI ENG TECH

Oncomelania screening equipment

The utility model discloses oncomelania screening equipment which comprises a rack and a screening assembly, the screening assembly comprises a support rotationally connected to the rack, the support is connected with an inclination angle adjusting mechanism, an annular screening hopper is arranged in the support, the screening hopper is in a horizontal state at the beginning, the rear end of the screening hopper is provided with a bottom, and a power motor for driving the screening hopper to rotate is arranged on the support. The front end of the screen hopper is open and provided with a screen cover capable of being opened and closed, a vibrating screen assembly connected with the rack is arranged below the support, and a collecting box is arranged below a screw outlet of the vibrating screen assembly. According to the oncomelania screening equipment, centrifugal force generated when the transverse cylindrical screening hopper rotates is adopted for conducting primary screening on oncomelania mud mixtures, secondary screening is further conducted through the vibrating screen assembly, and oncomelania large in size is screened out. The inclination of the screening hopper can be adjusted, feeding and foreign matter discharging are facilitated, the oncomelania can be cleaned in cooperation with high-pressure spraying in the screening process, and it is guaranteed that the surfaces of the screened oncomelania are cleaner.
Owner:NANJING COLLEGE OF CHEM TECH

Time-series in-situ zymography quantitative imaging method of soil enzyme activity based on multi-parameter screening

The application discloses a kind of time sequence in situ zymogram soil enzyme activity quantitative imaging methods based on multi-parameter screening, it is related to soil enzyme activity detection technical field, the method collects time sequence fluorescence image of enzymatic reaction, constructs pixel-level time sequence gray matrix;Multi-stage screening is carried out to pixel, including initial screening based on gray value and standard deviation, slope and goodness of fit screening of sliding window fitting, and reaction time window final screening;Extract maximum local linear growth rate as uncorrected enzyme activity, introduce diffusion correction model correction, obtain corrected enzyme activity;Quantitative conversion and visual output are realized in combination with fluorescence standard curve.This application changes static measurement into dynamic process analysis, effectively suppresses background noise, significantly improves the sensitivity, spatial resolution and quantitative accuracy of enzyme activity detection.
Owner:ZHEJIANG UNIV

Fish feed protease mutant with improved enzyme activity and preparation method thereof

The invention discloses a fish feed protease mutant with improved enzyme activity and a preparation method thereof, and belongs to the field of enzyme engineering. Error-prone PCR mutation is carried out on the basis of fish feed protease to obtain a mutant library, eight mutants (ER12, ER5, ER8, ER10, ER9, ER14, ER6 and ER4) with the protease activity remarkably improved are obtained through primary screening and secondary screening, and ER5 (the mutation site is N223S) and ER12 (the mutation site is S222P) are named as E0TYP4-2 and E0TYP4-3; the protease activity of the mutant E0TYP4-3 is about 2.26 times that of a wild type, and the protease activity of E0TYP4-2 is about 2.11 times that of the wild type. The enzyme activity of the mutant is greatly improved, and the mutant is more suitable for being used as a feed additive than a wild type, and is beneficial to wide application of fish feed protease in feed, so that the market prospect is wide.
Owner:GUANGDONG MING JI AQUATIC PROD CO LTD

Meyrowia guilliermondii strain with high yield of beta-glucan and application of Meyrowia guilliermondii strain

The invention relates to the technical field of strain breeding, in particular to a high-yield beta-glucan Meyrowia guilliermondii strain and application thereof. According to the present invention, the high-yield beta-glucan mutant strain obtained through ARTP mutagenesis has the name of Meyerozyma guilliermondii NCUASD7, has been preserved in Guangdong Microbial Culture Collection Center on November 28, 2025, and has the preservation number of CDMCC NO: 67365; aRTP mutagenesis is high in mutation rate, good in hereditary stability and safer to operate, a large number of mutation libraries can be obtained within a short time, high-throughput primary screening and shake flask secondary screening are conducted through a 24-deep-hole plate, a yeast mutant strain with the high beta-glucan yield capacity is obtained, then subculture is conducted on the high-yield mutant strain, the hereditary stability of the strain is verified, and the high beta-glucan yield is obtained. The mutant strain not only has the characteristic of high yield of beta-glucan, but also can maintain good genetic stability.
Owner:NANCHANG UNIV

A method and system for detecting a new pollutant of ecological environment

PendingCN122109042AEnsemble learningRaman scatteringImpedance responseAcute toxicity testing
The present application relates to the field of environmental monitoring and biosensing technology, and particularly relates to a detection method and system for new pollutants in ecological environment, comprising the following steps: S100: culturing specific effector cells in a biosensing channel, and collecting trans-epithelial electrical resistance (TEER) signals of the effector cells in real time; S200: monitoring a change rate of the TEER signals, and generating an enrichment triggering instruction when the change rate exceeds a preset threshold; S300: in response to the enrichment triggering instruction, introducing a fluid to be tested, which generates the change rate, into a surface-enhanced Raman scattering (SERS) detection cavity, and starting an active enrichment unit to in-situ concentrate the fluid to be tested, so that the volume of the fluid to be tested is contracted to a detection area of a SERS substrate; the present application uses impedance response of effector cells as a preliminary screening "sentinel", realizes targeted capture of only abnormal samples with acute toxicity, avoids ineffective detection of a large number of non-toxic water samples, and significantly improves monitoring efficiency.
Owner:SHENZHEN HUAMEI GREEN ECOLOGICAL ENVIRONMENT GRP CO LTD

A multi-stage screening device

ActiveCN224272003UGuarantee high efficiencyTo achieve the effect of clearing the blockageSievingScreeningAgricultural engineeringPrimary screening
This utility model discloses a multi-stage screening device, including a support box. A screening mechanism is rotatably connected to the top of the support box. The screening mechanism consists of a primary screening cylinder, a secondary screening cylinder, and a tertiary screening cylinder, installed sequentially from the inside out in order of increasing screen size (from fine to coarse). One end of the support box is equipped with a feeding platform that can feed materials into the primary, secondary, and tertiary screening cylinders respectively. This utility model involves pouring the material to be screened, such as crop seeds or ore fragments, into the interior of the primary screening cylinder. At this time, the primary, secondary, and tertiary screening cylinders rotate simultaneously on the support box to perform multi-stage screening of the material. During the screening process, because the primary, secondary, and tertiary screening cylinders are constantly rotating, the material is continuously adjusted in position on the screen, thereby clearing the screen and effectively preventing screen blockage, ensuring the high efficiency of multi-stage screening.
Owner:SICHUAN MEDCO PHARML

Automated Initial Screening Expert Judgment System for Endangered Species and Their Products

1. Name of the product in this design: Automatic Initial Screening Expert Judgment System for Endangered Species and Their Products. 2. Purpose of this design: To inspect endangered organisms and their products. 3. The key design features of this product are the combination of shape and pattern. 4. The image or photograph that best illustrates the design's key features: the front view. 5. The bottom surface of this design product is a part that is not easily seen or cannot be seen during use, so the bottom view is omitted; the top view of this design product is a part that is not easily seen or cannot be seen during use, so the top view is omitted.
Owner:SICHUAN JIELAIMEI TECHNOLOGY CO LTD +5

Method for in-situ ecological simulation of double-layer agar culture

PendingCN121518306ABacteriaMicrobial inoculationNutrition
The invention discloses a layered gel culture technology constructed by using environmental extracts, and belongs to the field of environmental microorganism culture. According to the technology, an environmental sample is separated into a particle phase and a liquid phase through water extraction, the particle phase and the liquid phase are respectively prepared into a lower-layer solid-phase matrix and an upper-layer gel phase with gel (preferably agar), a vertical nutrition / signal slow-release gradient is formed in a culture dish, and particle attachment sites are provided; and inoculating the environmental microorganisms on the upper layer or the surface for culture. Compared with nutrient agar or extremely simple water agar, the method has the advantages that more bacterial colonies and higher distinguishable form number can be obtained under the repeatable condition, and the community diversity and the OTU / ASV overlapping degree with an original sample are improved; rare / slow-growing groups can be stably separated, and subsequent 16S / metagenome analysis and agar block bacteriostasis primary screening are facilitated. Meanwhile, the invention provides a corresponding culture medium composition and a kit / prefabricated layered culture dish. According to the scheme, operation is easy and convenient, parameters can be standardized, and the method is suitable for isolated culture and ecological function research of environmental samples such as soil, sediments and low-solid water samples.
Owner:ZHENGZHOU UNIV