Disclosed are a prothioconazole and fluopicolide containing sterilization composition and uses thereof. The sterilization composition uses prothioconazole and fluopicolide as main active components. The sterilization composition can be used for preventing and curing diseases of cereal, fruit trees and vegetables, has a relatively high synergistic effect, overcomes and delays drug resistance of pathogens, has a fast sterilization speed and a long persistent period, reduces the application cost, and has an obviously more excellent prevention and cure effect than the prevention and cure effect when prothioconazole and fluopicolide are separately used. The present invention can be used for preventing and curing fungal diseases on crops, especially a powdery mildew disease, a sheath blight disease, a wilt disease, a leaf spot disease, a rust disease, a rhizoctonia disease, a net blotch disease, a leaf blotch disease, a gray mold disease, a black spot disease, a brown spot disease, a black shank disease, a rice blast disease and a phytophthora disease, and has a better effect than the effect when prothioconazole and fluopicolide are separately used.