78 results about "Molecular screening" patented technology

The invention discloses a biological dehydration method for garbage, which comprises piling garbage on a site, covering a molecular screening film on the garbage pile, and carrying out forced ventilation on the garbage pile until the dehydration is finished. The molecular screening film at least comprises an expanded teflon film layer, wherein the thickness of the expanded teflon film is 0.22-0.26 mu m, the micropore size is 0.5-1 mu m, and the number of pores is 10-20 billion/cm<2>. The molecular screening film is covered to biologically dehydrate the biochemical garbage, thereby effectively preventing malodorous gas in the pile from escape, ensuring that water can smoothly pass through the molecular screening film, quickly dehydrating the garbage pile and implementing a pollution-free processing environment.

The invention provides a preparation method of fragrant soft rice germplasms with low chalkiness and high resistance to rice blast. The preparation method comprises the following steps: hybridizing fragrant soft rice strains with high resistance to rice blast as female parents and high-quality long-grain fragrant japonica rice strains as male parents to obtain seeds F1; culturing the seeds F1 to obtain a first-generation selfing population, namely seeds F2; culturing the seeds F2, screening out strains with homozygous and/or heterozygous Pigm, Wx-mq and GL7 genes, long grains and good agronomic traits from the strains F2, and harvesting seeds F3 from all the strains; and culturing the seeds F3, screening out strains with homozygous Pigm, Wx-mq and GL7 genes, long grains and good agronomictraits from the strains F3, and continuously selfing the strains for multiple generations and identifying the rice blast resistance to finally obtain fragrant soft rice strains with low chalkiness andhigh resistance to rice blast. Step-by-step molecular screening is adopted to realize efficient transfer of genes with high appearance quality, so the novel fragrant soft rice strains with low chalkiness and high resistance to rice blast are obtained.

The invention provides a training method and a device for determining a neural network of a molecular inverse synthesis route. The method comprises the following steps: for a plurality of molecules, when the inverse synthesis route of each molecule is determined, a hierarchical learning concept is adopted, the training process of the molecular inverse synthesis route which needs to be explored toa large depth is split into multiple layers for training, and the complete inverse synthesis reaction process is replaced by the multiple layers of molecular inverse synthesis routes; after the training of one layer of molecular reverse synthesis route is completed, representative molecules are selected as starting molecules of the next layer of molecular reverse synthesis route by means of a molecular screening mode, so that the exploration efficiency of the molecular reverse synthesis route can be effectively improved, and the accurate cost value information of the molecules can be extractedmore efficiently. By means of the layering mode, a large amount of calculation expenditure caused by determination of the molecular reverse synthesis route is greatly reduced, and on the basis that the accuracy of the molecular reverse synthesis route is guaranteed, the time for determining the molecular reverse synthesis route is shortened.

The invention belongs to the field of gene technology and medicine, in particular to C9orf139 and MIR600HG as prognostic markers of pancreatic cancer and an establishment method of the markers. The pancreatic cancer prognosis markers comprise C9orf139 and MIR600HG. By exploring the differentially-expressed mRNA, lncRNA and miRNA in pancreatic cancer, a ceRNA network is constructed to find new molecular screening biomarkers for pancreatic cancer and provide new analytical and molecular mechanisms for lncRNAs, miRNAs and pancreatic cancer mRNA. The invention determines two new biomarkers as potential pancreatic cancer prognosis indicators, and thus the prognosis of pancreatic cancer diseases is improved, and the accuracy of early diagnosis of pancreatic cancer is improved.

The invention discloses application of JAK (Janus kinase) in the preparation of products for promoting induction of multipotent stem cells to generate megakaryocytes and platelets. A megakaryocytic differentiation system established herein has higher operability and repeatability and can provide efficient rapid in-vitro generation of active platelets. Rapid efficient in-vitro generation of platelets is achieved under serum-free stromal cell-free culture conditions; specific functional micromolecules are added in the megakaryocytic differentiation stage through small-scale molecular screening,and the ratio of active platelets (CD41a<+>CD42b<+>) is further increased. Statistical results show that the generation of 27-36 CD41a<+>CD42b<+> active platelets can be induced through single human polypotent stem cells, and the results are evidently higher than the results of the prior art. The rapid simple serum-free stromal-free culture strategy lays the basis for the large-scale production offunctional platelets for clinical therapy.

The invention discloses a molecule screening method which is suitable for being executed in computing equipment. The method comprises the following steps: obtaining a structure file of a target spot, the structure file being a ligand structure file, an acceptor structure file or an acceptor-ligand compound structure file, the ligand structure file comprising a plurality of ligand molecules, the acceptor structure file comprising acceptor molecules, and the acceptor-ligand compound structure file comprising ligand molecules and acceptor molecules; extracting a pharmacophore corresponding to the structure file as a pharmacophore model of the target spot; performing conformation search on each candidate molecule in the candidate molecule library to obtain a plurality of conformations corresponding to each candidate molecule; and aligning each conformation of each candidate molecule with the pharmacophore model to obtain a plurality of candidate molecules of which the alignment degree meets a predetermined condition as target molecules. The invention also discloses a computing device suitable for executing the method.

The invention relates to a molecular screening protector, comprising an outer housing. The molecular screening protector is characterized in that one end of the upper end of the outer housing is fixedly connected with an air inlet pipe, the other end is fixedly connected with an air outlet pipe, five baffle plates are connected in the outer housing, and efficient sorbents are filled between the baffle plates. Therefore, the molecular screening protector has the advantages of simple structure, increased service life of oxygenerator and easy repair, is specially used for absorbing water or oil in the air, and is particularly suitable for a small-size oxygenerator.

The invention discloses a glycosylnaphthalimide-containing fluorescent probe and application thereof in screening and cell imaging of a glycosidase inhibitor. The structure formula of the glycosylnaphthalimide-containing fluorescent probe is shown as formula I, a glycosyl part is beta-glucose, beta-N-acetylglucosamine, beta-galactose, beta-N-acetyllactosamine, and n is equal to 1 to 6. The glycosylnaphthalimide-containing fluorescent probe designed and synthesized in the invention has good water solubility and fluorescent performance, can be applied to the molecular screening of the glycosidase inhibitor, and can rapidly screen the inhibitor molecules with the inhibition rate greater than 70 percent by virtue of eyes; and the probe can be further used for the fluorescent imaging of the glycosidase in living cells.

The invention discloses a cellulose nanocrystal/nano-silver chiral nematic composite film. The cellulose nanocrystal/nano-silver chiral nematic composite film comprises cellulose nanocrystals and nano-silver. The mass ratio of the cellulose nanocrystals to the nano-silver is 11.5-5,049, the inside of the composite film adopts a chiral nematic liquid crystalline phase structure, and the thickness of the composite film is 0.1-1 mm. The composite film has excellent circular dichroism and can be used in anti-counterfeiting labels, asymmetric organic synthesis, chiral molecular screening and otherfields. The preparation method of the composite film comprises steps as follows: a cellulose nanocrystal suspension was prepared with a sulfuric acid hydrolysis method, a nano-silver solution was prepared with a redox method, the cellulose nanocrystal suspension is mixed with the nano-silver solution, and ultrasonic treatment, evaporation and self-assembly for film forming are performed. Accordingto the method, nano-silver is induced by cellulose nanocrystals to form the chiral nematic composite film with strong circular dichroism, so that the production cost is reduced and green and non-toxic production is realized.

The present invention provides a molecular breeding method of cold-tolerant rice. The molecular breeding method comprises: (1) carrying out hybridization by using a rice restoring line as a female parent and using a cold-tolerant rice line as a male parent to obtain F1 seed; (2) carrying out backcrossing on the rice restoring line as a recurrent parent and the hybridization F1 to obtain a first backcrossing generation group BC1 seed; (3) culturing the BC1 seed; and (4) screening the line containing the cold-tolerant gene from the BC1 lines, carrying out backcrossing on the obtained line and the rice restoring line, and carrying out selfing to obtain the rice line containing the cold-tolerant gene. According to the present invention, the excellent cold-tolerant rice resource is used, the new gene is excavated, the step-by-step molecular screening of the backcrossing group cold-tolerant material is performed, and the cold-tolerant material and the phenotype of the cold tolerance of the hybrid generation can be identified, such that the efficient transfer of the cold-tolerant gene can be achieved so as to create the new cold-resistant rice material.

The invention discloses an application of BAMBI as a nasopharyngeal carcinoma maker. Through experiment researches by an applicant and a whole team for three years, researches on tissues and serums of 38 nasopharyngeal carcinoma patients, 12 chronic nasopharyngitis patients and 49 healthy volunteers are carried out, the researches show that the nasopharyngeal tissue BAMBI expression amount of the nasopharyngeal carcinoma patients is obviously increased, the expression amount of nasopharyngitis (nasopharyngitis is common benign disease) is relatively low, wherein p equals 0.0038, and the difference between nasopharyngeal carcinoma and nasopharyngitis is obvious. The serum BAMBI of the nasopharyngeal carcinoma patients is obviously increased, through statistical analysis, t equals 2.639, and p equals 0.012. No matter in nasopharyngeal carcinoma tissue or nasopharyngeal carcinoma serum, BAMBI presents up-regulated expression, so that the detection of BAMBI can be taken as a nasopharyngeal carcinoma molecular screening index.

The invention discloses a building method for a liver cancer rat model. The method comprises the steps that 1, animals are included, wherein the clean class is Wistar, the number of male rats is 40, the weight is 150+/-20 g, and the male rats are randomly divided into an experimental group including 30 rats and a control group including 10 rats; the animals are reared in a semi-barrier system according to the raising requirement of the clean class of the animals; 2, modeling is performed, wherein sterile tap water is used for preparing a diethylnitrosamine solution with the concentration being100 microgramPml (the solution is kept in a dark place and freshly prepared), the solution is freely drunk by the rats in the experimental group, the solution is changed one time every day, after three months, the solution is replaced with ordinary sterilization tap water, observation continues to be performed for 2 months, and the whole experimental process lasts 5 months; according to the control group, the rats drink the sterilization tap water in the whole experiment process; 3, liver cancer is determined; 4, liver tissue is sampled. A clinic sample is adopted for performing positioning and quantified expression verification, the evidence related clinically is found, the clinic value is evaluated, and the novel clue is provided for liver cancer pathogenesis and liver cancer mechanismresearch. The liver cancer key molecule screened by a question for discussion is adopted for researching a liver cancer marker related to early discovery, classification and evaluation prognosis, andthe research basis is laid for selecting a more effective and accurate liver cancer treatment target position.

The invention belongs to the technical field of plant genetic engineering and provides a method for obtaining a salt-tolerant soybean new material by utilizing a transgenic technology. The method comprises the following steps: (1) introducing a plant expression vector pYL-AgGlpF containing a gene AgGlpF into a receptor soybean genome by utilizing agrobacterium tumefaciens-mediated soybean cotyledonary node genetic transformation method; and (2) carrying out molecular screening and salt tolerance identification, so that a transgene AgGlpF salt-tolerant soybean material is obtained. The method provided by the invention has the advantages that a gene AgGlpF is transferred into the soybean genome, and salt tolerance of soybeans can be obviously improved, so that the method provided by the invention has important economic values and broad application prospects on agricultural production.

The invention discloses a kit for simultaneously detecting 33 respiratory tract pathogens through a single reaction hole of an MALDI-TOF mass spectrum platform. The kit comprises multiple PCR amplification primers and single-basic-group extension primers. Other markers such as fluorescein and biotin do not need to be added into the designed primer groups, and the good sensitivity, stability and accuracy are achieved in detection of pathogen nucleic acid sequences; the MALDI-TOF mass spectrum platform has extremely high accuracy and extremely high speed, both sample loading detection and result analysis can be automated, a reaction system only needs common oligonucleotides and does not need fluorescence labeling, the labor cost and the detection cost are low, and the platform is suitable for large-scale population molecular screening and conventional gene diagnosis; and according to the kit, the 33 respiratory pathogens are detected in the same reaction system, the flexibility and expandability are both realized, the operation is simple, the flux is high, the cost is low, and the methodological research trend and the practical demand of the core problem of rapid detection of pathogen nucleic acid can be effectively met.

The invention provides a method for carrying out early-stage molecule screening of breast cancer by utilizing two methylation sites in a control region of a gene DFNA 5. In a methylation microarray data analytical process on two breast cancer cell lines, results show that methylation of two CpGs in the control region of the gene DFNA 5 is obviously lower than that of other genes, the demethylation of the gene DFNA 5 is supposed to be one important characteristic of pathogenesis of the breast cancer, and the two methylation sites can be used as one marker used for early-stage screening of the breast cancer. The marker provided by the invention can be possibly a cheap marker used for carrying out early-stage molecular screening of the breast cancer.

The invention discloses a molecular screening method based on a deep learning technology qualitative and quantitative model (EMIM), which comprises the following steps: converting collected molecules into SMILES, and then converting the SMILES into pretreatment of qualitative and quantitative descriptors; carrying out hash mapping, variance threshold and Pearson's correlation coefficient feature engineering on qualitative and quantitative descriptors; setting a qualitative and quantitative model according to input data processed by feature engineering, using a Sigmoid function as final output, optimally setting parameters of the qualitative and quantitative model through a back propagation optimization algorithm to perform performance evaluation, and performing iterative training to obtain a model with high verification precision; enabling a to-be-predicted molecule to be subjected to preprocessing and feature engineering and then inputting the to-be-predicted molecule into the highest verification precision qualitative and quantitative model (EMIM) for prediction screening, and obtaining a prediction result of the to-be-predicted molecule. According to the method, more efficient and accurate screening of new molecules is realized, and the limitation of traditional screening is solved.

Pap smears and HPV infection tests do not distinguish between lesions that will progress to an invasive carcinoma and those that will not. We aimed to identify epigenetic biomarkers for diagnosis and progression monitoring of premalignant lesions in cervical cancer. Hypermethylated genes were identified as potential biomarkers after validation by MSP, including GGTLA4 and ZNF516. The methylation frequency for these two genes was higher in tumor: GGTLA4 (100%) and ZNF516 (96%); than in normal samples: GGTLA4 (12%) and ZNF516 (16%). The methylation status of GGTLA4 showed a progression in methylation frequency from normal samples to invasive carcinoma. The immunohistochemical expression was lower in tumor for both: GGTLA4 (50.8%) and ZNF516 (66.2%); than in normal samples: GGTLA4 (71.2%) and ZNF516 (88.1%) (p<0.05). In conclusion, we identified methylation biomarkers for the molecular screening and characterization of cervical cancer.

The invention provides synthesis of a two-dimensional coordination polymer based on a structure inducer. The powder X-ray diffraction peak positions of the coordination polymer are as follows: 10.06+/-0.5, 11.85+/-0.5, 26.55+/-0.5, 27.97+/-0.5, and 36.12+/-0.5, a 2theta angle range is between 5 DEG-60 DEG. The invention provides the two-dimensional coordination polymer based on the structure inducer, which uses amphoteric pyridinium oxygen negative ions as the structure inducer, and transition metal copper ions and benzene tertacarbonic acid are coordinated to form a novel two-dimensional coordination polymer. A two-dimensional coordination polymer film is obtained by ultrasonic stripping; the two-dimensional polymer film has molecular screening performance and can selectively screen Rhodamine B; the special dye organic matter in water can be separated, and the water body can be purified; the molecular screening method is more efficient by comparing with photolysis of an organic dye inthe water body, the secondary pollution caused by the decomposition of organic dye can be avoided, the raw materials are cheap and easy to obtain, and the reaction conditions are mild, which is beneficial to industrial production.

The invention provides a screening kit for paroxysmal supraventricular tachycardia. The screening kit comprises an optional reagent for detecting a human CACNA1B gene c.1700 A>G mutation site, whereinthe c.1700A>G mutation site of the CACNA1B gene refers to a mutation site where the 1700th basic group of the coding region of the CACNA1B gene is changed from A to G. The kit provided by the invention is designed based on the significant correlation between the c.1700A>G mutation of the CACNA1B gene and paroxysmal supraventricular tachycardia; the screening kit for paroxysmal supraventricular tachycardia is a first molecular screening kit for paroxysmal supraventricular tachycardia, can assist in diagnosis of paroxysmal supraventricular tachycardia (especially atrioventricular nodal reentrant tachycardia), is beneficial to prenatal and postnatal care, can guide doctors to apply medicines according to symptoms and has good application prospects.

The invention mainly relates to a classical swine fever virus Erns protein affinity polypeptide and application. The polypeptide is designed by a computer-simulated virtual molecular docking technology, and the sequence is WRHYIH. According to the invention, a polypeptide sequence P1 is obtained according to a virtual molecular screening technology by taking a swine fever Erns protein crystal structure obtained by homologous modeling as a template. After the polypeptide is artificially synthesized, an enzyme-linked immunosorbent assay test and a plasma resonance test are employed to identify the affinity of the interaction of the sequence and the swine fever Erns protein, and the result shows that the sequence P1 and the swine fever Erns protein have relatively high specificity and affinity; and the invention provides a reliable theoretical basis for affinity chromatography taking affinity peptide as an affinity ligand, and swine fever Erns protein can be rapidly detected by labeling the polypeptide.

The invention relates to the field of plant biotechnology breeding, in particular to an OsNramp5 mutant as well as a screening method and application thereof. The invention discloses an OsNramp5 mutant, and a nucleotide sequence for coding the OsNramp5 mutant comprises a sequence as shown in SEQ ID NO. 1 or SEQ ID NO. 2. The mutation rate of the plant genome is increased by adopting radiation mutagenesis, the mutation of the OsNramp5 allele is detected from the radiation mutagenesis population by applying a high-throughput molecular screening technology in the seedling stage of the plant M2 generation after mutagenesis, and the screening efficiency and accuracy of the OsNramp5 mutant are improved. The OsNramp5 mutant is screened out, and compared with a wild plant, the cadmium content in seeds of the plant containing the OsNramp5 mutant is reduced by 90% or above.