43 results about "Molecular screening" patented technology

The invention discloses development and application of a molecular marker of a rape stain tolerance related gene BnaERF3, and belongs to the technical field of molecular biology. According to the invention, the BnaA06. ERF3 gene is screened on the basis of whole genome associated molecules, and is used as a key gene in QTL (qWL.A06) to participate in regulation and control of the waterlogging tolerance of the rape. A molecular marker related to the waterlogging tolerance of the brassica napus is developed aiming at the BnaA06. ERF3 gene, and tests show that the waterlogging tolerance of the brassica napus can be detected by utilizing the molecular marker, so that varieties containing waterlogging tolerance genes are subjected to auxiliary selection and breeding, and the waterlogging tolerance breeding process of the brassica napus is accelerated.

The invention discloses an SERS (Surface Enhanced Raman Scattering) substrate with a needle-like diagram nanostructure and a preparation method of the SERS substrate, and belongs to the field of nanotechnology and detection. The preparation method of the needle-like Turing nano-structure SERS substrate comprises the following steps: by taking a silver needle as a carrier, carrying out electrochemical etching on the surface of the silver needle to induce to form a Turing nano-structure with fractal topological characteristics, and then inducing to conformally grow a layer of zeolite imidazole framework-8 material as a shell layer by utilizing the nano curvature characteristics of the fractal structure, so as to prepare the needle-like Turing nano-structure SERS substrate. And a composite SERS enhanced platform with an electromagnetic field hot spot aggregation effect and a molecular screening and enriching capability is constructed. The platform has the advantages of high structural uniformity, expandability in manufacturing, synergy of signal enhancement and molecular recognition and the like, remarkably improves the detection sensitivity and the signal reproducibility, and is suitable for trace analysis in a complex sample system, such as related application in the fields of food safety, environmental pollution monitoring, biomedicine and the like.

The invention relates to application of mitogen-activated protein kinase PiPmk1 in screening molecules for inhibiting formation of a phytophthora infestans infection structure. The invention finds that the molecular marker plays a key role in the development of a phytophthora infestans cyst bud tube and / or the formation of an infection structure, which indicates that the molecular marker has the potential of serving as a target for inhibiting phytophthora infestans infection, and a corresponding molecular screening method and a generative prediction model are further developed and utilized; rapid and accurate prediction and screening of phytophthora infestans infection inhibitory molecules are realized, the research and development period and cost are greatly shortened, and a new thought and a new method are provided for development of accurate targeted green pesticides.

The invention relates to the technical field of graded screening, in particular to a graded screening system based on staggered stacked metal ring films and a preparation method and application. An organic ligand and two metal complexes are subjected to interface coordination assembly under the action of silver ions, an ordered staggered stacked metal ring film is constructed, the structure and performance of the metal ring film are precisely regulated and controlled, the metal ring film has a definite molecular sieve component difference, and then precise grading screening of different molecules is achieved. The film has an obvious lattice structure, has good crystalline orderliness, and can ensure the stability and reliability of the screening process. The graded screening system based on the staggered stacked metal ring membranes has excellent interception performance on target molecules, and effective separation of TAC from glucan and leucine can be achieved. The two-stage membrane classification screening strategy can also be popularized to separation and purification of other substances, and good universality and industrial application prospects are achieved.

The invention provides an SNP (Single Nucleotide Polymorphism) molecular marker related to nocardia seriolea seriolea resistance traits of lateolabrax japonicus, a method for screening nocardia seriolea seriolea resistance lateolabrax japonicus and application, and particularly belongs to the technical field of aquatic organisms. The invention provides an SNP (Single Nucleotide Polymorphism) molecular marker related to nocardia seriola seriola resistance traits of lateolabrax japonicus, the nucleotide sequence of the SNP molecular marker is as shown in SEQ ID NO.1, the 760th basic group of the sequence as shown in SEQ ID NO.1 is an SNP site, and the basic group is A or G; when the SNP molecular marker is AA genotype, the lateolabrax japonicus is judged as the lateolabrax japonicus resistant to nocardia seriolae. The SNP molecular marker is an effective SNP marker for early molecular screening of nocardia seriolae seriolae infection resistance traits of lateolabrax japonicus, disease resistance breeding of lateolabrax japonicus can be achieved, and nocardia seriolae seriolae resistance lateolabrax japonicus is obtained.

The invention discloses a molecular screening method and device, a medium and a product. The method comprises the following steps: acquiring multiple terminal groups and multiple intermediate molecular fragments; generating a plurality of molecules based on the plurality of terminal groups and the plurality of intermediate molecule fragments, and describing each molecule by adopting a molecular structure file; according to each molecular structure file, obtaining parameter values of a plurality of performance parameters of each molecule; and screening out a target molecule from the plurality of molecules based on the parameter values of the plurality of performance parameters of each molecule. Through the mode, the multiple generated molecules can be quickly screened, the target molecules meeting the requirements are obtained, the human participation degree in the whole process is low, the cost can be reduced, and the molecular screening efficiency is improved.

The invention relates to the field of rotating wheel adsorption, and particularly discloses zeolite molecule screening rotating wheel adsorption concentration equipment which comprises a frame, a rotating disc rotationally mounted on the frame and a plurality of adsorption filtering units distributed in the circumferential direction of the rotating disc. The adsorption filtering unit comprises an outer supporting pipe, a composite filter and a zeolite disc; the outer supporting pipe is arranged along the radius direction of the turntable; the composite filter is movably mounted in the first end of the outer supporting pipe; the zeolite plate is mounted in the second end of the outer supporting pipe; four treatment areas which are distributed in the circumferential direction are arranged on the rotary table, and first multi-way connectors and second multi-way connectors which are installed on the frame are arranged on three of the treatment areas; the first multi-way connector is used for being connected into the first end of the outer supporting pipe, and the second multi-way connector is used for being connected into the second end of the outer supporting pipe. The device has the effect of being easy to maintain.

The invention discloses development and application of a molecular marker of a rape oil content related gene BnaLTP3.A02, and belongs to the technical field of molecular biology. According to the invention, the BnaLTP3.A02 gene is screened on the basis of whole genome associated molecules, and the BnaLTP3.A02 gene is used as a key gene in QTL (qeOC.A02.1) to participate in regulation and control of the oil content of brassica napus. The molecular marker related to the oil content of the brassica napus is developed aiming at the BnaLTP3.A02 gene, and experiments find that the oil content of the brassica napus can be detected by utilizing the molecular marker, so that auxiliary selection and breeding of varieties with high oil content are realized, and the process of high-oil-content breeding of the brassica napus is accelerated.

Described herein are methods and systems for proposing coordination framework compounds, such as crystalline porous materials, crystalline open frameworks, network chemical compounds, metal-organic framework (MOF) compounds, covalent organic framework (COF) compounds, zeolitic imidazole framework (ZIF) compounds, and combinations thereof. Also described herein are coordination frame compounds produced by these methods and systems and adsorbent systems comprising these coordination frame compounds. The methods and systems described herein combine machine learning and chemistry to propose chemically effective and improved performance coordination framework compounds that meet the different objectives of material discovery.

This invention discloses the development and application of a molecular marker for the BnaERF3 gene associated with waterlogging tolerance in rapeseed, belonging to the field of molecular biology. Based on genome-wide association molecular screening, the BnaA06.ERF3 gene was identified as a key gene in a QTL (qWL.A06) involved in regulating waterlogging tolerance in rapeseed. A molecular marker associated with waterlogging tolerance in Brassica napus was developed for the BnaA06.ERF3 gene. Experimental results indicate that this molecular marker can be used to detect waterlogging tolerance in Brassica napus, thereby enabling assisted selection and breeding of varieties containing the waterlogging tolerance gene and accelerating the breeding process for waterlogging tolerance in Brassica napus.

The invention belongs to the technical field of biological medicines, and particularly relates to application of an angiotensin II receptor antagonist in preparation of a medicine for preventing and treating renal tubular injury caused by triptolide. According to the invention, the upstream key target protein of the TPL-induced renal tubule injury is determined to be HSPA1 through experiments for the first time, and the problem that the key target spot of TPL toxicity is not determined in the prior art is solved. Based on a key target spot of TPL toxicity, through molecular screening and parallel pharmacodynamic verification on various sartan drugs such as irbesartan, losartan, olmesartan and valsartan, it is determined that the angiotensin II receptor antagonist has a general protection effect on TPL toxicity. Wherein the protective efficacy of irbesartan as a preferable embodiment is obviously superior to that of losartan of the same kind (the efficiency slope is 2.451 to 1.584).

The invention provides a high-throughput molecule screening and molecule intelligent construction platform and a data processing method thereof, and relates to the technical field of chemical informatics and drug research and development, the high-throughput molecule screening and molecule intelligent construction platform comprises a multi-molecule batch operation module, a single-molecule editing module and a modular task combination engine, 34 core functions of molecular data cleaning, multi-dimensional screening, molecular fingerprint similarity analysis, similarity map screening based on MACCS fingerprints, structure replacement, molecular disassembly, atomic contribution analysis and the like are supported; according to the data processing method, a unique SMILES code is generated through standardized cleaning, in combination with screening conditions such as user-defined functional groups and molecular weights, a similarity matrix is calculated by utilizing topology / MACCS fingerprints, and a reference molecular structure is matched through a similarity map, so that high-precision screening is realized. The problems of limited application scene, poor database compatibility and complex operation in the prior art are solved, and the molecular screening efficiency and precision are remarkably improved.

The present invention discloses a method for detecting halogenated organic substances in a landfill leachate and water bodies polluted with the leachate, by a combined solid phase extraction method comprising HLB solid phase extraction and PPL solid phase extraction, acidifying the sample using formic acid, and subjecting the aqueous sample to solid phase extraction enrichment using an HLB solid phase extraction column and a PPL solid phase extraction column, respectively, elution with methanol, the enriched samples are then subjected to FT-ICR MS detection, performing molecular formula matching of organic molecules, molecular screening, molecular information summarization and statistical analysis of data of samples separately on two FT-ICR MS detection data, collecting the two together gives all dissolvable halogenated organic compounds (HOCs) in samples of water, halo-organic species are then visualized by Sankey diagrams.

The invention discloses a screening method, system and device based on molecule generation and activity prediction and a medium. The screening method comprises the following steps: S1, collecting an active molecule sample; s2, preprocessing the collected active molecule samples to generate standardized molecule data; s3, predicting the activity of the standardized molecular data; and S4, screening the standardized molecular data. The method is suitable for molecular screening in the field of drug discovery, and compared with the prior art, the method has the following advantages that efficient generation is achieved, specifically, molecules related to specific targets are rapidly generated through transfer learning, and the experimental screening range is reduced; the ROC values of the activity prediction model on a BindingDB database and a DAVIS database respectively reach 0.968 and 0.989, so that the screening precision is remarkably improved. And comprehensive screening: combining a molecular docking technology to further verify a molecular binding mode and improve the experiment success rate. In conclusion, the method is particularly suitable for active molecule discovery of novel targets (such as STING agonists), the research and development period can be remarkably shortened, and the experiment cost can be reduced.

The invention relates to the technical field of animal husbandry genetic breeding, in particular to an SNP (Single Nucleotide Polymorphism) molecular marker and a primer pair related to the strength of an egg shell of white coming aviation and application of the SNP molecular marker and the primer pair. Compared with the prior art, the white leghorns with the ultra-long egg producing period are subjected to whole genome association analysis, SNP sites related to the eggshell strength character are screened out, then the SNP sites rs733980419 which reach the whole genome significant level at the age of 60 weeks are screened out through single-site GWAS analysis, the SNP molecular marker is developed on the basis of the screened SNP sites, and the SNP molecular marker has the advantages that the SNP sites related to the eggshell strength character are obtained; experiments prove that the SNP molecular marker is closely related to the eggshell strength, early molecular screening is realized, and the breeding efficiency is improved. Experiments prove that the SNP molecular marker and the primer pair provided by the invention can be used for early identification or screening of eggshell strength traits of chicken flocks and auxiliary breeding of chicken strains with high eggshell strength traits, and the eggshell breakage rate in the later period is reduced.

The invention relates to the technical field of drug research and development and artificial intelligence crossing, and particularly discloses an intelligent drug research and development molecular screening method. The method comprises the following steps: constructing a bipartite interaction diagram of candidate molecules and target protein; for each interaction edge in the graph, generating a dynamic interaction feature representing the specific molecule-target pair potential binding mode in real time through an interaction attention module as an edge feature; a graph neural network is used for multi-round message passing, dynamic interaction features are iteratively refreshed according to updated node features, and collaborative optimization of node and edge features is achieved; and finally, calculating a prediction score based on the optimized edge features and node features to complete screening. According to the method, interaction characteristics are dynamically generated and iteratively optimized, so that the dynamic process of molecule-target combination is effectively simulated, and the applicability and the screening accuracy of targets with incomplete structural information are improved.

This invention discloses a method for heavy ion beam mutagenesis breeding of poultry. To address the challenges of efficient, rapid, and safe breeding in poultry, this method utilizes heavy ion beam irradiation of poultry progenitor cells (PGCs) to induce radiation mutagenesis, constructing a mutant cell library. The PGCs in this library are then isolated into single cells to form monoclonal populations. Targeted deep sequencing is used to screen for positive PGCs with gene mutations from the S2-after monoclonal population, and the mutated gene is preliminarily identified. Positive PGCs are injected via microinjection into recipient embryos after their own PGCs have been ablated, resulting in chimeras. Individuals developed from these chimeras undergo molecular screening and identification. M1 generation individuals containing the identified mutated gene are then used for breeding to obtain offspring strains that stably inherit the mutated gene. This method is efficient, rapid, safe, and avoids the ethical and regulatory risks associated with gene editing technologies, making it of significant application value for mutagenesis breeding in poultry.

The application discloses a method for increasing drought adaptability of plant osmotic regulation substance accumulation, and belongs to the field of plant genetic engineering and drought-resistant molecular breeding. First, a plant to be transformed is combined with 13C-glutamic acid and 15N-choline double isotope labels to quantize carbon and nitrogen flow redistribution of proline synthesis pathways and glycine betaine synthesis pathways in leaf cells and vascular bundle companion cells, and to identify rate-limiting enzyme nodes. Second, a multi-cistronic plant expression vector is constructed. A first expression box is driven by a leaf-specific promoter and a drought response cis-element composite promoter to drive P5CS. A second expression box is driven by a companion cell-specific promoter and a drought response cis-element composite promoter to drive BADH. Positive transformants are obtained through Agrobacterium mediation or gene gun transformation and molecular screening. The expression quantity of the positive transformants is increased by 5 to 50 times under stress and falls back to a baseline within 72 hours of rehydration. Root activity is significantly improved, and yield is preserved.

The application discloses a needle-shaped Turing nanostructure SERS substrate and a preparation method thereof, and belongs to the field of nanotechnology and detection. The preparation of the needle-shaped Turing nanostructure SERS substrate is specifically as follows: a silver needle is used as a carrier, a Turing nanostructure with fractal topological characteristics is induced to form by electrochemical etching the surface, then, a layer of zeolite imidazole framework-8 material is induced to conformally grow as a shell layer by using the nano-curvature characteristics of the fractal structure, and a composite SERS enhancement platform with electromagnetic field hot spot gathering effect and molecular screening enrichment capacity is constructed. The platform has the advantages of high structural uniformity, scalable manufacturing, signal enhancement and molecular recognition synergy, and significantly improves the detection sensitivity and signal reproducibility, and is suitable for trace analysis in a complex sample system, such as related applications in the fields of food safety, environmental pollution monitoring and biomedicine.

The invention discloses a preparation method of recombinant III-type collagen based on fish maw, and relates to the technical field of biologication.The preparation method comprises the steps that firstly, the fish maw is hydrolyzed through an acidic hydrolysis method, fish maw collagen hydrolysate is obtained, then collagenase is added for an enzymolysis reaction, III-type collagen is obtained through extraction, then neutral salt is added for a salting-out reaction, and the recombinant III-type collagen is obtained. The preparation method comprises the following steps: preparing a III-type collagen peptide solution by using collagen as a raw material, then utilizing an enzymatic recombination technology and a cross-linking reaction of enzyme to obtain a recombinant III-type collagen peptide solution, and finally concentrating, purifying and drying the recombinant III-type collagen peptide solution to obtain recombinant III-type collagen freeze-dried powder. According to the method, a mode of combining temperature-controlled enzymolysis and acidolysis is adopted, the structure of collagen is prevented from being damaged by high temperature through low-temperature extraction and activity retention, so that the biological activity, high purity and high activity of the collagen are effectively retained, and the recombinant III type collagen peptide with high purity and high activity is obtained through membrane filtration, molecular screening and recombination technologies.

The application provides a molecular strain energy calculation method, a molecular screening method and related devices. The calculation method comprises the following steps: determining all rotatable dihedral angles of a target molecule based on an initial conformation of the target molecule; performing one-dimensional scanning on each rotatable dihedral angle to obtain a one-dimensional potential energy surface conformation set corresponding to each rotatable dihedral angle; calculating a first energy value of each first conformation in each one-dimensional potential energy surface conformation set to determine a first conformation set corresponding to a local energy minimum point in each one-dimensional potential energy surface conformation set; generating a pseudo two-dimensional scanning conformation set according to the first conformation sets; calculating a second energy value of each pseudo two-dimensional scanning conformation; determining a global energy minimum target conformation in the first conformation sets and the pseudo two-dimensional scanning conformation set according to the first energy value and the second energy value; and determining the strain energy of the target molecule based on an initial energy value of the initial conformation and a target energy value of the target conformation. The application improves the calculation efficiency of the strain energy.

The invention relates to a method for screening an interleukin-8 antagonist and a drug application of the interleukin-8 antagonist, and the method for screening the interleukin-8 antagonist comprises the following steps: S1, customizing a specific interleukin-8 as a target, and customizing an FDA drug library as a drug molecule library for later use; s2, performing high-throughput molecular screening in a drug molecule library by using the target, and screening specific drug molecules capable of being combined with the target from the drug molecule library; and S3, detecting the antagonism effect of the specific drug molecule on the target, and performing target antagonism agent-effect evaluation on the specific drug molecule.

The invention belongs to the field of medical chemistry and fine chemical synthesis, and relates to a borate-substituted aryl sulfane compound as well as a preparation method and application thereof. And carrying out photochemical reaction on the compound I and the compound II under the action of a base catalyst to prepare the borate-substituted aryl sulfane compound III. The borate-substituted aryl sulfane compound shown in the formula III has an antagonistic effect on a thromboxane A2 receptor, and can be used for molecular screening of drugs for treating cardiovascular diseases and asthma and preventing lung shock. The unique free radical migration mechanism of the method provided by the invention opens up a new dimension for molecular diversity expansion in drug discovery and precise modification in subsequent industrial products and material science, and the newly synthesized borate substituted aryl sulfane compound provides more possibilities for drug molecule design and modification. And a solid foundation is laid for subsequent drug research and development.

This invention discloses a heavy ion mutagenesis breeding method for fish, belonging to the field of fish breeding technology. To address the technical problem of how to efficiently apply mutagenesis breeding technology in fish genetic improvement, this method first breeds the target fish species to obtain fertilized eggs in the single-cell stage; then, it uses a heavy ion beam to induce mutagenesis in the single-cell stage fertilized eggs, hatches and raises the mutagenized eggs to obtain an M1 generation population; next, it performs molecular screening to identify M1 generation individuals carrying one or more gene mutations; finally, it uses breeding methods to obtain offspring strains that can stably inherit the stated gene mutations. This invention shortens the breeding cycle from several generations to as short as 1-2 generations, significantly improving screening efficiency and providing an efficient, precise, and universally applicable pathway for rapidly creating new germplasm and strains of various fish species.

The application provides a SNP molecular marker related to a Nocardia seriolae resistance trait of Epinephelus akaara, a method for screening the Nocardia seriolae resistance Epinephelus akaara and application, and specifically belongs to the field of aquatic organism technology. The application provides a SNP molecular marker related to a Nocardia seriolae resistance trait of Epinephelus akaara, wherein the nucleotide sequence of the SNP molecular marker is shown in SEQ ID NO. 1, the base at the 760th position of the sequence shown in SEQ ID NO. 1 is a SNP site, and the base is A or G; when the SNP molecular marker is an AA genotype, it is determined that the Epinephelus akaara is resistant to Nocardia seriolae. The SNP molecular marker provided by the application is an effective SNP marker for early molecular screening of the Nocardia seriolae resistance trait of Epinephelus akaara, can realize disease resistance breeding of Epinephelus akaara, and obtain Epinephelus akaara resistant to Nocardia seriolae.

The invention relates to the technical field of plant molecular breeding, and particularly discloses application of a tomato SlAOS gene as a positive regulatory factor in regulating tomato response to high VPD. Through construction of an overexpression and gene editing strain of the SlAOS gene, it is verified that the gene can enhance the responsiveness of a plant to high VPD by regulating jasmonic acid (JA) synthesis, and it is shown that stomata closing is accelerated, the leaf water retention rate is increased, and the transpiration effect is reduced. Furthermore, a molecular selection marker taking SlAOS as a core is constructed and is used for screening a high-VPD-resistant tomato material. The invention provides a new molecular target and application strategy for genetic improvement of tomato stress resistance characters under facility agriculture conditions, and has good popularization and application prospects.

The invention discloses an SNP (Single Nucleotide Polymorphism) marker and a KASP primer group for detecting resistance of a wildfire tobacco No. 0 physiological race and application of the SNP marker and the KASP primer group. The invention firstly discloses an SNP (Single Nucleotide Polymorphism) marker for detecting the resistance of the wildfire tobacco 0 # physiological race, the SNP marker is positioned at the 111102247 position of the 22 # chromosome of a tobacco genome, and the nucleotide type of the SNP marker is A or G. The invention further discloses a KASP primer group for detecting the SNP marker and application of the KASP primer group. The SNP marker disclosed by the invention is a tobacco growth resistance site, 80%-90% of non-target materials can be eliminated through molecular screening in the seedling stage in the breeding process, the manpower and material resource cost of field management and phenotype identification is remarkably reduced, and the SNP marker has a wide application prospect.

The invention discloses an ultrasonic water molecular screening machine, and relates to the technical field of water molecular screening. The ultrasonic technology is adopted, instant separation of water molecules and other substances is achieved through high-temperature and high-pressure impact generated by breakage of the nano bubbles, the surfaces of the water molecules are gasified and recombined, finally, purified water with the same flow is obtained, and the nano bubbles are generated in the nano tubes; water molecules are forced to be separated from other substances by high-temperature and high-pressure impact generated when bubbles are broken on the surfaces of nano screening holes, after a water molecular chain is broken, the surface is gasified and recombined into an H9O4 + N hydrated oxyhydrogen molecular structure, H9O4 + N hydrated oxyhydrogen purified water is obtained through nano screening, and the cold distillation process is completed; during backwashing, nano bubbles are generated in the screener through ultrasonic waves, other substance molecules are forced to be separated from the surface of the screener through high-temperature and high-pressure impact generated when the nano bubbles are broken on the surfaces of nano screening holes, the substances are discharged out of the screener through a blow-off pipe, and the automatic cleaning process is completed.

The invention relates to a screening method and application of a longleaf menthone interleukin-8 antagonist. The screening method of the longleaf menthone interleukin-8 antagonist comprises the following steps: S1, customizing a drug molecule library, and customizing interleukin-8 as a target; s2, performing high-throughput molecular screening in a drug molecule library by using a target, and screening drug molecules capable of being combined with the interleukin-8 target from the drug molecule library; and S3, carrying out in-vitro antagonistic activity verification on the drug molecules screened in the step S2 so as to detect the antagonistic effect of the specific drug molecules on the target effect, and carrying out agent-effect evaluation of the specific drug molecules on the antagonistic interleukin-8.

The invention discloses a molecule generation method and device based on a generative adversarial network, equipment, a medium and a product, and relates to the technical field of computational chemistry and batteries. The method comprises the following steps: constructing a molecule database by adopting a matrix engineering method according to atom types and quantity required by target molecules; converting a molecular structure corresponding to the candidate molecular set in the molecular database into a graph structure to obtain a molecular graph database; inputting the molecular graph database into the trained generative adversarial network, and performing molecule generation and decoding to obtain newly generated molecules; based on a preset screening rule, performing molecular screening on the newly generated molecules to obtain screened qualified molecules; converting the screened qualified molecules into three-dimensional structures to obtain three-dimensional molecules; the three-dimensional molecules are used for computational simulation and experimental verification to provide a reference basis for electrolyte design and optimization. According to the invention, molecule generation and screening can be efficiently realized.