53 results about "Jasmonic acid" patented technology

The invention belongs to the technical field of biological agents, and particularly relates to ginsenoside variant flavobacterium DG-16 and application thereof in relieving continuous cropping obstacles of angelica sinensis and improving the quality of the angelica sinensis. According to the ginsenoside variant flavobacterium DG-16 disclosed by the invention, the growth of angelica sinensis is promoted by increasing the contents of jasmonic acid and cytokinin hormones, reducing the contents of gibberellin and salicylic acid hormones and promoting the circulation and utilization of nitrogen; in addition, the flavobacterium ginsenoside variant DG-16 can significantly increase the chlorophyll content, is beneficial to photosynthesis of the angelica sinensis, effectively changes the physiological and biochemical states of the angelica sinensis, can resist root rot of the angelica sinensis so as to relieve successive cropping obstacles and improve the quality, and has a significant effect.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to a promoter of a pinus massoniana terpene synthase gene Pm TPS (-)-alpha-pinene and application of the promoter. According to the invention, a promoter fragment of a Pm TPS (-)-alpha-pinene gene and three homeopathic action elements with jasmonic acid response on the promoter fragment are obtained from pinus massoniana. A complete promoter fragment of the Pm TPS (-)-alpha-pinene gene of the pinus massoniana is transformed into arabidopsis thaliana by means of agrobacterium mediation, and the promoter can drive expression of a GUS gene in leaves of the arabidopsis thaliana or nicotiana benthamiana. GUS staining and GUS enzyme activity of transgenic Bensi tobacco leaves are reduced along with deletion of jasmonic acid response cis-acting elements on the promoter. Furthermore, the yield of pine resin terpenoids can be increased by utilizing the promoter and the homeopathic element thereof.

The application provides a kind of dihydro jasmonic acid propyl ester soluble powder type that can be compounded with powder fertilizer and a preparation method and application thereof.The powder type includes the following components by weight percentage: dihydro jasmonic acid propyl ester 5-15%, solvent 0-15%, emulsifier 20-50% and filler 35-75%.The application is simple to operate, raw materials are more easily obtained, and mixing and compounding are more convenient.It can be compounded with powder water-soluble fertilizer and high-salinity solution to obtain a mixture, and the mixture can be used in various application scenarios such as foliar spraying, flushing, root irrigation, and root leaching, making it more suitable for large-scale market promotion.It provides more application methods and application scenarios for dihydro jasmonic acid propyl ester and nutrient element compounding to promote fruit quality improvement and shorten the ripening period.

The invention belongs to the technical field of biology, and particularly relates to a gene SbAIF1 for enhancing broad-spectrum disease resistance of sorghum and application of the gene SbAIF1. It is proved for the first time that the SbAIF1 gene is over-expressed in sorghum, the broad-spectrum disease resistance of sorghum is enhanced by increasing the jasmonic acid content in sorghum, a new thought is provided for cultivating disease-resistant transgenic plants, and the SbAIF1 gene has important production significance and life significance for crop breeding and production application.

The invention discloses a method for advancing the fruit maturation period of snowflake pears, and relates to the technical field of fruit and vegetable planting. According to the method, 15 days before fruit harvesting, a fruit ripening promoting agent is sprayed to fruit trees; the fruit maturation promoting agent is any one of 200 [mu] mol / L melatonin, 100 [mu] mol / L jasmonic acid or a mixture of 200 [mu] mol / L melatonin and 100 [mu] mol / L jasmonic acid. According to the method, the arbutin content and the soluble solid content in the early-harvested snowflake pear fruits can be increased, the hardness and the titratable acid content are reduced, and the change of the indexes is synthesized to powerfully prove that the snowflake pears are matured in advance.

The invention discloses application of pseudomonas aeruginosa T17 in prevention and treatment of rice bacterial blight. The invention discloses pseudomonas aeruginosa T17 and a function of a fermentation broth thereof as a biological agent in the aspect of disease control. Experiments prove that the pseudomonas aeruginosa T17 can directly inhibit growth of bacterial blight pathogenic bacteria (Xoo) and pyricularia oryzae (M.oryzae). Meanwhile, the fermentation product filtrate can directly induce up-regulated expression of rice defense related genes and up-regulated expression of jasmonic acid (JA) synthesis key genes OsAOS2, and the resistance of rice to bacterial blight can be enhanced by pretreating rice with the fermentation product filtrate. Therefore, the research lays a foundation for taking the pseudomonas aeruginosa T17 and the fermented filtrate thereof as the rice immune resistance inducer, and provides a new thought for developing innovative pollution-free botanical pesticides.

The application relates to the technical field of pharmaceutical analysis, and discloses a pharmacokinetic analysis method of Tongwei Xiaozheng medicinal liquid, which adopts Q-Orbitrap high-resolution liquid chromatography-mass spectrometry to analyze the concentration of Jasmonic acid in blood components of the Tongwei Xiaozheng medicinal liquid in rat plasma, establishes a plasma concentration determination method for the blood original component Jasmonic acid, carries out methodological verification such as specificity, precision and accuracy, and then calculates pharmacokinetic parameters. In the Tongwei Xiaozheng medicinal liquid, 92 blood components are identified, 17 of which are original components and 75 of which are metabolites, the application firstly determines that Jasmonic acid is one of the blood original components of the Tongwei Xiaozheng medicinal liquid, and reveals the pharmacokinetic characteristics of the Jasmonic acid in normal and model rats. The method has strong specificity, high sensitivity and good reproducibility, and can provide a scientific basis for the research on the pharmacodynamic material basis of the Tongwei Xiaozheng medicinal liquid, the establishment of quality control standards and the clinical rational use of drugs.

The invention discloses an underground propagule composite excitant for ecological restoration and a preparation method thereof, and relates to the technical field of excitant preparation. The fertilizer is prepared from the following raw materials in parts by weight: 0.2 to 0.3 part of gibberellin, 0.2 to 0.5 part of 6-benayl aminopurine, 0.2 to 0.3 part of indolebutyric acid, 0.5 to 0.7 part of sodium nitroprusside, 0.4 to 0.5 part of hydrogen peroxide, 1 to 2 parts of jasmonic acid sustained-release microspheres, 5 to 7 parts of compound amino acid, 1 to 3 parts of compound microbial agent sustained-release microspheres, 2 to 3 parts of seaweed extract, 10 to 13 parts of humic acid, 2 to 3 parts of monopotassium phosphate, 60 to 74 parts of ethanol and 1000 parts of water. The underground propagule composite excitant provided by the invention is beneficial to promoting activation and germination of underground propagules, enhancing plant growth and stress resistance, improving soil micro-ecological functions, forming stable diversity communities and facilitating ecological restoration.

The invention discloses an insecticidal composition containing entomopathogenic virus and jasmonic acid methyl ester or jasmonic acid, and relates to the technical field of biological insecticide, the active ingredients of the insecticidal composition are entomopathogenic virus and jasmonic acid methyl ester or jasmonic acid, and the entomopathogenic virus is nuclear polyhedrosis virus of baculoviridae. The weight ratio of the virus to the jasmonic acid methyl ester or jasmonic acid is 1: (0.5-1000), and the insecticidal composition can be prepared into dosage forms such as a suspending agent, water dispersible granules, wettable powder and the like. The invention also provides a method for preventing and treating lepidoptera pests, which comprises the step of applying the entomopathogenic virus and methyl jasmonate or jasmonic acid to target pests or a living environment of the target pests in a combined manner, and the combined application is simultaneous application or successive interval application. The insecticidal composition has a synergistic effect on lepidoptera pests such as cotton bollworms and beet armyworms, can obviously reduce the food intake, accelerate the death of pests and improve the field control effect, and has a wide application prospect.

The application provides a method for constructing a regulation network of a potato JA pathway in response to Cd stress. The method relates to the technical field of data processing, and comprises the following steps: obtaining multi-omics time series data of potatoes under the conditions of cadmium stress and methyl jasmonate treatment; performing signal decomposition and feature purification on the multi-omics time series data by using a weak stationary signal decomposition method based on sequence patterns to obtain a plurality of intrinsic mode components; constructing a dynamic regulation network model to capture multi-scale change patterns of response characteristics in the time dimension and the concentration dimension, thereby extracting deep features; inputting the intrinsic mode components into the trained dynamic regulation network model to output an attention weight matrix, thereby forming a regulation network representing the dynamic regulation intensity of the jasmonic acid pathway; and solving the optimal exogenous methyl jasmonate concentration for promoting biomass and inhibiting cadmium accumulation by optimizing an objective function. The application provides a high-precision model tool for analyzing the regulation mechanism of plant hormone signals under heavy metal stress.

The invention discloses a method for enhancing pine wood nematode disease resistance of larch through jasmonic acid signal mediated flavonoid biosynthesis. The method comprises the following steps that 1, the larch is inoculated with pine wood nematode; 2, performing high-throughput omics sequencing sampling and data analysis; 3, treating larch seedlings with methyl jasmonate; 4, the inhibition effect of the kaempferol and the citrus aurantium on the pine wood nematodes is obtained. According to the method, larch is taken as a research object, a disease stress system is constructed by artificially inoculating pine wood nematodes, leaf / stem tissues at different time points after infection are selected, and sequential multiomics analysis is carried out by combining transcriptome sequencing and a wide targeting metabolome technology; the theoretical basis and method for improving the pine wood nematode disease resistance of larch by regulating and controlling the biosynthesis of kaempferol and citrus aurantium through a jasmonic acid signal way are defined.

This invention relates to the field of microbial technology, and particularly to a peanut-specific growth regulator and its application in sugarcane-peanut intercropping. The peanut-specific growth regulator of this invention consists of 1 g / L-3 g / L of GX-2-sugarcane leaf microbial growth regulator, 2 g / L-6 g / L of GX-7-peanut vine microbial growth regulator, 0.2 g / L-0.4 g / L of brassinolide, and 0.2 g / L-0.4 g / L of jasmonic acid. At this concentration, the peanut-specific growth regulator can significantly increase peanut yield. Under sugarcane-peanut intercropping conditions, the yield increase is even more significant. This peanut-specific growth regulator can provide a new approach for the development of specialized agricultural biological agents.

This invention belongs to the field of bio-agricultural technology, specifically relating to genes. DISC1 The application of its encoded proteins in regulating plant resistance to Phytophthora parasiticans. This invention, through the study of... DISC1 Studies on the function of genes and their encoded proteins have revealed that... DISC1 Negative regulation of plant resistance to Phytophthora parasiticum. Overexpressed genes. DISC1 Significantly reduces plant resistance to Phytophthora parasiticum, gene DISC1 The mutant plants exhibited significantly enhanced resistance to Phytophthora parasiticum. Further research into the immune mechanism revealed that the gene... DISC1 This invention regulates plant immune responses by modulating genes related to the jasmonic acid (JA) signaling pathway. It provides a novel gene resource for the control of plant resistance to Phytophthora parasiticum.

This invention discloses a method for cultivating enoki mushrooms using camellia peel as a culture medium, belonging to the field of enoki mushroom cultivation technology. The method includes the following steps: (1) Pretreatment: crushing and sieving camellia peel to obtain camellia peel residue; (2) Degradation treatment: pre-wetting camellia peel residue with water, adding mixed inoculant, wheat bran, sucrose, quicklime, and degradation agent, and fermenting to obtain culture medium; (3) Substrate treatment: adding jasmonic acid to the culture medium, treating it under high temperature and high pressure, and then drying it to obtain the treated culture medium; (4) Mushroom bag making: packing the treated culture medium into bags, inoculating enoki mushroom spawn under aseptic conditions to obtain mushroom bags; (5) Mushroom cultivation: placing the mushroom bags in a cultivation room for mycelial cultivation, and managing mushroom cultivation after the mycelium has fully grown; (6) Harvesting: generally, the stipe can be harvested when it reaches 13-14cm in length and the cap diameter is 0.8-1.5cm.

The invention relates to the technical field of fertilizers, in particular to application of a composition containing jasmonic acid to improvement of root rot resistance of southern rhodiola roots. The jasmonic acid-containing composition prepared by the invention has a remarkable effect on improving the root rot resistance of the southern rhododendron. Experimental results show that after the plant is treated by the compound, the resistance of the plant to fusarium oxysporum infection is obviously enhanced, the disease time is delayed, and the disease infection degree is obviously reduced. Field application further proves that the treatment can effectively improve the survival rate of the plants and reduce the susceptibility rate, has no adverse effect on the yield of the plants, and shows good agronomic applicability.

The application belongs to the technical field of crop disease prevention and treatment, and discloses application of salicylic acid in prevention and treatment of apple replant disease, adopts 0.1mmol / L-10mmol / L salicylic acid aqueous solution to root irrigation treatment of apple plant rhizosphere soil, and the optimal application concentration is 10mmol / L; through synergistic effect of exogenous salicylic acid from three levels of plant growth physiology, rhizosphere microecological regulation and root molecular response, the growth and photosynthesis indexes of apple seedlings in replant soil can be restored to the level of healthy soil, the rhizosphere pathogenic microorganism abundance is significantly reduced, and beneficial bacteria are enriched, and at the same time, the root system differential gene expression is restored at the transcription level, and core defense pathways such as jasmonic acid signal are regulated. The prevention and treatment method is green, efficient, simple to operate and low in cost, and provides a new way for prevention and treatment of apple replant disease, and can effectively guarantee the yield and quality of apples.

The present application provides a kind of to improve the nutrition fertilizer of yam stress resistance and its preparation and use method, the nutrition fertilizer includes base fertilizer and conditioning agent, the base fertilizer includes jasmonic acid-chitosan oligosaccharide conjugate, amino mesoporous silica nanoparticles, basic nutrient component, auxiliary material, binder, the basic nutrient component includes slow-release nitrogen source, readily available nitrogen source, phosphorus source, potassium source, trace element;The conditioning agent includes modified attapulgite, gamma-polyglutamic acid, sodium dodecyl sulfate.The nutrition fertilizer provided by the present application is not only comprehensive nutrition, and yam stress resistance can be effectively improved, and then the yield and quality of yam are improved.

This invention provides a bHLH transcription factor involved in the regulation of camptothecin synthesis in *Sedum pachycarpa*. OpJRB1 And its applications. OpJRB1 Transcription factors are induced by jasmonic acid, highly expressed in roots, and can bind to repressors in the jasmonic acid pathway. OpJAZ1 , OpJAZ5 and OpJAZ6 Interaction, fully explaining OpJRB1 Transcription factors are involved in the jasmonic acid signaling pathway. Knockout OpJRB1 The gene significantly reduces the camptothecin content in the hairy roots of *Hedyotis diffusa* and is overexpressed. OpJRB1 Genes can increase camptothecin content. OpJRB1 Transcription factors can transiently activate the camptothecin synthesis gene. OpTDC1 and OpLAMT1 Gene expression is enhanced, thereby increasing the accumulation of camptothecin. This invention provides a novel, high-quality drug source for the production of camptothecin with broad-spectrum anticancer efficacy, possessing significant theoretical importance and potential application value.

The invention provides penicillium chrysogenum capable of inducing plants to generate disease resistance, and belongs to the technical field of biocontrol bacteria. The invention provides penicillium chrysogenum. The penicillium chrysogenum and phytophthora nicotianae do not have antagonism. After the spore suspension of the penicillium chrysogenum is sprayed on crops, gene expression can be regulated and controlled from salicylic acid, ethylene and jasmonic acid ways, active oxygen accumulation is reduced, and meanwhile, defensive enzyme activity is improved, so that plants are induced to generate resistance to pathogenic bacteria. The pre-inoculation of penicillium chrysogenum reduces the morbidity of tobacco black shank to a certain extent and slows down the expansion speed of black shank scabs, the treatment of inoculation of penicillium chrysogenum 1 day in advance has a good control effect on tobacco black shank, and the relative control effects are up to 42.86% and 27.08% in 3 days and 7 days.

This invention provides a cytochrome P450 gene HvCYP94C1 and its application in improving the resistance of highland barley to powdery mildew, belonging to the field of agricultural biotechnology. The cytochrome P450 gene HvCYP94C1 of this invention can hydroxylate JA and JA-Ile to 12-OH-JA-Ile and 12-COOH-JA-Ile, respectively. Silencing this gene in powdery mildew-sensitive materials can significantly increase the jasmonic acid content, thereby improving the disease resistance of the materials, showing excellent application prospects.

This disclosure provides a method for alleviating the damaging effects of D. maidis in corn crops, which involves the use of plant defense elicitors such as jasmonates and salicylates.

The invention discloses a method for endogenously improving peel wax content and prolonging the shelf life of tomato fruits, belongs to the field of biological genes, and aims to solve the problems that continuous or high-concentration treatment is needed for prolonging the shelf life of the tomato fruits by regulating expression of wax metabolism related genes of the tomato fruits through exogenous jasmonic acid in the prior art. And the obvious effect can be maintained. Through overexpression of the SlC3H73 gene, the endogenous jasmonic acid content of tomato fruits is reduced. Meanwhile, the thickness of cuticles of the tomato fruits can be remarkably increased, the storage resistance of the tomato fruits is improved, and therefore the tomato germplasm with the storage resistance is created.

The invention provides a seed soaking agent for enhancing stress-resistant growth of tomato seedlings in a high-temperature environment as well as a preparation method and application of the seed soaking agent, and belongs to the technical field of seed soaking agents. The seed soaking agent comprises boric acid, jasmonic acid, gamma-aminobutyric acid, cane sugar, proline and water. The boron element is an important micronutrient element for plant growth and development, and participates in cell wall formation, glycometabolism and membrane structure stability maintenance; jasmonic acid is a plant hormone and participates in regulation and control of defense reaction of plants to adversity and activation of an anti-oxidation system; the gamma-aminobutyric acid as a non-protein amino acid can be quickly accumulated in the plant body to effectively maintain ion balance, remove active oxygen and stabilize the cell membrane structure, so that the heat resistance of the plant is enhanced. The three components are compounded, and cane sugar and proline are supplemented to construct the compound seed soaking agent, so that the stress-resistant growth performance of tomato seedlings in a high-temperature environment can be enhanced.

The invention belongs to the technical field of biological agriculture, and particularly relates to application of a gene DISC1 and an encoding protein thereof to regulation and control of phytophthora parasitica resistance of plants. The functional research on the DISC1 and the encoded protein thereof finds that the gene DISC1 negatively regulates the resistance of plants to parasitic phytophthora. The over-expressed gene DISC1 significantly reduces the resistance of the plant to phytophthora parasitica, and the phytophthora parasitica resistance of the gene DISC1 mutant plant is significantly enhanced. The further study on the immune mechanism finds that the gene DISC1 regulates and controls the plant immune response by regulating and controlling the jasmonic acid (JA) signal channel related genes. The invention provides a new gene resource for preventing and treating parasitic phytophthora diseases of plants.

This invention relates to the field of crop genetics and breeding technology, and particularly to a rapid breeding method for high-CBD industrial hemp. The invention provides a rapid breeding method for high-CBD industrial hemp, which involves constructing a high-segregation-potential F1 generation base population through high-purity parental selection and reciprocal crosses; using oleic acid combined with methyl jasmonic acid to construct a targeted metabolic stress screening system for active ingredient synthesis; completing only one in vitro targeted screening in the F1 generation to identify the core target genotype; then continuously backcrossing to aggregate the superior traits of the recurrent paternal parents, ultimately selecting a line with high CBD content; furthermore, subsequent backcross generations are completed using greenhouse short-day generation combined with rapid, non-destructive in vivo detection technology, eliminating the need for repeated tissue culture operations; finally, after self-pollination purification, a target line with stable traits is obtained.

The invention discloses a tea tree exogenous induced insect-resistant agent based on synergistic interaction of jasmonic acid methyl ester and salicylic acid as well as a preparation method and application of the tea tree exogenous induced insect-resistant agent, and belongs to the technical field of biological control. Based on the synergistic interaction of jasmonic acid methyl ester and salicylic acid, tea geometrid and tea lesser leafhopper are repelled, and parasitic wasps and hoverflies in a tea garden are lured. Synergistic interaction is achieved through the specific proportion of jasmonic acid methyl ester and salicylic acid, a tea tree jasmonic acid and salicylic acid double insect-resistant signal pathway is activated, the total amount and the component fraction of volatile matter generated through induction are remarkably higher than those generated through single elicitor treatment, the population number of pest natural enemy parasitic wasps in a tea garden can be increased by 1.52 times through field tests, and the pest control effect is good. The number of hoverfly populations is increased by 1.33 times, the number of tea lesser leafhopper populations is reduced by 40.5%, the number of tea geometrid populations is reduced by 60%, and the insect-resistant effect can be maintained for 15 days.

The invention relates to the technical field of agricultural microbiology and phytopathology, in particular to application of rhodopseudomonas palustris in prevention and treatment of rice blast, by spraying a bacterial suspension of the strain with the concentration of 1 * 10 < 8 > CFU / mL to leaf surfaces of rice in the three-leaf one-core stage, a rice linolenic acid metabolic pathway can be regulated and controlled, and the rice blast resistance is improved. The accumulation of an endogenous disease-resistant metabolite 12-oxygen-plant dienoic acid is obviously induced. The OPDA inhibits the magnaporthe oryzae through a dual mechanism, the area of a plate inhibition zone reaches 94.5% + / -2% when the concentration is 50 micrograms / mL, the spore germination inhibition rate reaches 97.3% + / -1.2% (repeating for three times), and a rice jasmonic acid defense system can be activated. The disease-resistant molecular mechanism is defined, the control effect is equivalent to that of chemical pesticides, and a new strategy is provided for developing efficient and stable microbial-source rice blast-resistant preparations.

The invention belongs to the technical field of plant disease prevention and control, and particularly relates to application of a plant source compound preparation to prevention and control of tea tree anthracnose. The plant source compound preparation is prepared from plant secondary metabolites and a plant resistance inducer; the mass ratio of the plant secondary metabolite to the plant resistance inducer is 1: (0.1-10000); the plant secondary metabolite is selected from at least one of galactitol, 1-methyl nicotinate, caffeine, anthocyanin and quercetin; the plant resistance inducer comprises any one of methyl jasmonate, jasmonic acid and salicylic acid. The plant source compound preparation provided by the invention can realize disease prevention and control through double ways of direct bacteriostasis and host immunity activation, the prevention and control effect can be remarkably improved, and the use amount of each component is reduced, so that the production cost and the pollution to the environment are reduced.

This invention provides a strain of Flavobacterium ZJUHULHQ1 and its application in controlling *Pseudomonas gramineus* root-knot nematodes, belonging to the field of biological control technology for root-knot nematodes. The Flavobacterium ZJUHULHQ1 is deposited at the China Center for Type Culture Collection (CCTCC), accession number CCTCC NO: M 20251179. The Flavobacterium ZJUHULHQ1 can enhance plant resistance by increasing the content of the defense hormones salicylic acid, jasmonic acid, and jasmonic acid-isoleucine in rice, thereby inhibiting the invasion of *Pseudomonas gramineus* root-knot nematodes and reducing the number of root knots. Simultaneously, the Flavobacterium ZJUHULHQ1 has a certain growth-promoting effect on rice and has broad application prospects.

The present invention provides methods of use of hexokinase 2 (HK2) / mitochondria-detaching compounds, including jasmonate derivatives and piperazine derivatives and pharmaceutical compositions including such compounds for treating, inhibiting, or suppressing a hexokinase-2 (HK2)-expressing cancer.