290 results about "Jasmonic acid" patented technology

Lipo-chito oligosaccharides (LCOs) are produced by culturing rhizobacteria cells in or on a culture medium comprising at least one of: jasmonic acid or a derivative thereof; linoleic acid or a derivative thereof; or linolenic acid or a derivative thereof. Preferably, the rhizobacteria cells are Bradyrhizobium japonicum cells having the identifying characteristics of B. japonicum strain USDA 3. Preferably, the derivative of jasmonic acid is an ester thereof, preferably methyl jasmonate. Also provided are methods for improving LCO production at low temperatures, particularly temperatures below 25° C.

Described is a bioprocess for the high-yield production of food flavor-acceptable jasmonic acid and methyl jasmonate, as well as a novel jasmonic acid isomer produced thereby and organoleptic uses thereof. The process yields at least 5% of the "cis" isomer defined according the structure:(wherein R is hydrogen or methyl) or at least 5% of the "cis" isomer defined according to the structure:(wherein R is hydrogen or methyl). Compositions containing at least 98% of the isomer having the structure:with an optical rotation (alphaD20) of +58° are novel. Compositions containing at least 98% of the isomer having the structure:with an optical rotation (alphaD20) of +58° are also novel. The process of our invention comprises the cultivation under aerobic condition of one or more specific strain of Diplodia gossypina in a nutrient medium followed either by (1) isolation of the jasmonic acid product or (2) esterification of the jasmonic acid to form methyl jasmonate followed by the isolation of the methyl jasmonate and novel products produced by such process.

The invention discloses a plant growth regulating composition and its application. The composition comprises the following components: A) natural abscisic acid, B) gibberellic acid (GA3, or of GA4 +7), and C) an existing plant growth regulator. The existing plant growth regulator is selected from one or more than two of 6-BA (6-benzylaminopurine), thidiazuron, brassinolide, sodium nitrophenolate, potassium nitrophenolate, chlormequat chloride, triacontanol, naphthlcetic acid and its salt, indolebutyric acid and its salt, indoleacetic acid and its salt, ethephon, diethyl aminoethyl hexanoate, paclobutrazol, uniconazole, fulvic acid, Mepiquat chloride, nitrohumic acid, jasmonic acid and triapenthenol. Specifically, the components A), B) and C) are in a weight ratio of 1:0.001-500:0.001-500. The S-ABA (S-abscisic acid) added in the invention can expand the prevention and treatment objects, reduce or prevent phytotoxicity, reduce dosage, and lower the use cost.

The invention discloses a pesticidal foliage fertilizer, which is prepared by grinding and uniformly mixing gibberellin 3, heteroauxin, brassinolide, abscisic acid, jasmonic acid, zeatin, salicylic acid, 1-aminocyclopropane-carboxylic acid. The fertilizer is a novel phytosterol foliage fertilizer with advantages of high efficiency, no toxicity and environmental friendliness, can promote the growth of plants by over 10 percent, and has no environmental pollution.

The invention provides a method for inducing fresh water chlorella to fast accumulate grease through plant hormone jasmonic acid. The method is characterized by comprising the following steps of: (1) preparation of chlorella solution preparation: under the conditions that the temperature is 22-24 DEG C, the light intensity is 1800-2000lx, and the light-to-dark ratio is 12h / 12h, culturing fresh water chlorella to logarithmic phase to obtain chlorella solution for conducting hormone induction, wherein the fresh water chlorella adopts NO.9 fresh water chlorella or NO.31 fresh water chlorella which both come from Freshwater Algae Culture Collection at the Institute of Hydrobiology, FACHB-collection; (2) acquisition of high-grease-content chlorella: adding jasmonic acid into 200ml of chlorella solution obtained in step (1) until the final concentration is 0.49-0.51mg / l; and (3) acquisition of high-grease-content chlorella: culturing the chlorella solution subjected to hormone induction in step (2) for 15 days under the conditions that the temperature is 22-24 DEG C, the light intensity is 1800-2000lx, and the light-to-dark ratio of 12h / 12h, and centrifugally collecting the frond with high grease content. The method is simple and easy, low in cost, and the grease content of the target product is high, and can be used as a raw material of industrialized biological produced oil.

The invention discloses a tomato S1lPIF4 gene, protein and application thereof to improving the low temperature resistance of plant. The nucleotide sequence of the S1lPIF4 gene is SEQ ID NO.1, and thecorresponding amino acid sequence is as shown in SEQ ID NO. 2. A tomato SlIPIF4 overexpression plant or a gene knockout plant through the gene means to regulate the expression level of the S1lPIF4 gene in order to do research on the regulatory mechanism of the S1lPIF4 gene on the low temperature resistance of tomato. According to the result, overexpression of S1lPIF4 at the lower temperature canpromote accumulation of plant hormone abscisic acid (ABA) and jasmonic acid (JA), inhibits accumulation of gibberellin (GA), further induces expression of the low temperature resistant gene of the tomato, and finally improves the low temperature resistance of the tomato. Therefore, the S1lPIF4 gene of the tomato enhances the low temperature resistance by inducing formation of hormones ABA and JA in the body. The tomato S1lPIF4 gene provides the gene resource for breeding new varieties of low temperature resistant tomato, has the good potential application value, and lays theoretical basis fordoing research on tomato plant distress response signal mechanism and molecular mechanism of being tolerant to adverse environment.

The invention relates to a natural plant growth regulator as well as a preparation method and application thereof. The regulator contains the following components: 60-95 parts of plant distillate, 0-4.0 parts of glycine, 0-3.5 parts of glutamic acid, 0-2.0 parts of gamma-aminobutyric acid, 0-1.5 parts of salicylic acid and 0-0.5 part of jasmonic acid; moreover, total dosage of the glycine, the glutamic acid, the gamma-aminobutyric acid, the salicylic acid and the jasmonic acid is more than 0 part. The regulator, in which major components are derived from plant extracts, has a characteristic of being pure natural; by regulating a physiological status of plants, improving a physiological function of the plants and supporting healthy energy, the regulator can improve the plant productivity and a capacity for resisting external adverse environment so as to achieve a purpose; by enhancing an osmotic adjustment ability of plants as well as a water use efficiency and a cell membrane stability of the plants, capacities of early weaning and responding to adverse environment of the plants are enhanced, so that purposes of improving drought resistance, salt resistance and cold resistance of the plants are achieved; moreover, the regulator can inhibit diseases and insect pests and can resist continuous cropping.

By applying a compound selected from jasmonic acid represented by the following general formula (I) or 2-substituted form of jasmonic acid or derivatives thereof or salts thereof, the pharmacological action of a pesticide such as a microbicide or a herbicide applied to the plant is enhanced. (wherein R1 is an alkyl group or an alkenyl group, and R2 is a hydrogen atom, an alkyl group, an alkenyl group, an alkynyl group or a hydroxyalkyl group).

The present invention relates to a plant growth regulator composition, and is characterized by that in addition to the convertional inert processing adjuvant it contains the following active components: A). natural abscisic acid; B), nicotinamide; and C). at least one plant growth regulating material selected from jasmonic acid, methyl jasmonate or brassinolide.

The invention discloses a special culture medium for culturing yew plant cells to produce taxol, which is obtained by taking B5 culture medium as a basic formula and utilizing an orthogonal optimization test. When the basic component comparison production is carried out on the special culture medium and the B5 culture medium, the content of the taxol in the special culture medium is 4.2 times that of the B5 culture medium; when high-density of yew plant cells are planted, a combined test is carried out by adding silver nitrate, salicylic acid, chitosan, methyl jasmonic acid, tribasic ammonium citrate, lanthanum sulfate, phenylalanine, glycine, tryptophan and lysine in the culturing process, which can further improve the content of the taxol and shorten the production period of cells; and when the cells are cultured at the thirty-first day, the highest content of the taxol in the special culture medium is 285.15 mg / L which is 81 times the highest content of the taxol in the B5 culture medium, thus the taxol can be effectively produced by using the special culture medium for culturing yew plant cells to produce taxol.

Composition containing at least one foaming anionic or amphoteric surfactant and at least one compound derived from jasmonic acid. Methods of use.

The invention discloses a plant stress-tolerance-associated protein TaDREB3A and a coding gene and application thereof. The protein provided by the invention is one of the following proteins: (a) a protein consisting of an amino acid sequence shown as a sequence 1 in a sequence list, and (b) a protein which is associated with plant stress tolerance and is derived from the sequence 1 by substituting and / or deleting and / or adding one or several amino acid residues for the amino acid sequence shown in the sequence 1. The TaDREB3A provided by the invention can express under the induction of drought, high salt, high temperature, low temperature, pathogenic bacteria, ABA (Abscisic Acid), ethylene, JA (Jasmonic Acid) and SA (Salicylic Acid), and can specifically regulate and control transcription expression of the gene containing a DRE / CRT (Dehydration-Responsive Element / C-Repeat) cis-element (core sequence: CCGAC); therefore, the drought resistance, the salt tolerance, the high temperature resistance and the resistance to the powdery mildew pathogen of the plant are improved. The TaDREB3A provided by the invention provides a base for artificially controlling the expression of genes related to the stress tolerance and the stress resistance, and is about to play an important role in cultivation of plants with enhanced stress tolerance and enhanced stress resistance.

The invention relates to a DCPTA-containing (2-(3,4-dichlorophenoxy)triethylamine containing) plant growth regulation composition, comprising active ingredient A and active ingredient B; the active ingredient A is DCPTA; the active ingredient B is selected from any one of brassinolide, gibberellic acid, 2-diethylaminoethyl hexanoate, indoleacetic acid, harpin Ea, jasmonic acid, methyl jasmonate, and allantoin; the DCPTA-containing plant growth regulation composition is mainly used for increasing activity of plant endonuclear, increasing the contents of chlorophyll, protein, nucleic acid, juice, oil and lipoid in plants, maintaining green and preventing aging, significantly enhancing photosynthetic effect in crops, promoting development of roots, promoting effective absorption of fertilizer by crops, increasing yield and income, assisting in good growth of subordinate parts of crops, enhancing water-fertilizer absorption by plants, increasing the accumulative storage of proteins, lipids and the like, and promoting cell division and growth.

The invention discloses a corn planting method. The corn planting method comprises the following steps of: soaking seeds: treating seeds in a mixed seed soaking solution consisting of 50-100 parts by weight of garlic juice, 10-20 parts by weight of ethanol and 40-80 parts by weight of water; carrying out fertilization application treatment: before sowing, applying a base fertilizer to a corn planting region; sowing: sowing the corn seeds with the mixed seed soaking solution in the corn planting region which is applied with the base fertilizer; carrying out light treatment: sowing the seeds into soil, respectively carrying out light treatment on corn plants at a three-leaf stage, a heading stage, a flowering and milking stage and a dough stage of corn growth, wherein the specific process of the light treatment is as follows: irradiating the corn plants for 4-5 hours with yellow light with light intensity of 3000-4000 1x after a sunset per day, each period lasting for 10-15 days; and carrying out growth regulation treatment: carrying out growth regulation treatment on the corn plants at the later stage of the heading stage, and spraying a mixed solution of jasmonic acid and a leaf potassium fertilizer to the plants every 2-3 days.

The present invention discloses a method for increasing the fucoxanthin content of Phaeodactylum tricornutum by using methyl jasmonic acid. The method comprises: mixing sterilized seawater and an MAD mother liquor to obtain a nutrition liquid, adding a Phaeodactylum tricornutum mother liquor to the nutrition liquid to culture, culturing for 2 days, adding methyl jasmonic acid, continuously culturing for 6 days, collecting the algae liquid, carrying out centrifugal separation, removing the supernatant, collecting the algae, carrying out a freeze-drying treatment on the algae for 2 days, and grinding for spare. According to the present invention, with the method, the fucoxanthin content in the Phaeodactylum tricornutum can be significantly increased, the operations are simple and convenient, the cost is low, the production cycle is short, and the method is suitable for batch production and industrial production.

The invention relates to a plant growth regulating composition containing jasmonic acid and methyl jasmonate. The plant growth regulating composition contains an active component A and an active component B, wherein the active component A is jasmonic acid or methyl jasmonate, and the active component B is any one selected from a group consisting of benzyl aminopurine, forchlorfenuron, ethephon, tetramethyl glutarate, mepiquat chloride and choline chloride. The plant growth regulating composition is mainly used for improving enzyme activity, promoting seed germination, enhancing germination capacity and photosynthetic intensity, increasing the content of chlorophyll, maintaining greenness, preventing ageing, promoting development of roots and effective fertilizer absorption of crops, assisting the inferior part of a crop in good growth, enhancing absorption of water and fertilizer by plants and adjusting the balance of moisture in plants so as to improve drought resistance and cold resistance of plants; and the plant growth regulating composition can improve immunity, disease and insect resistance and crop quality and increase yield and income.

The invention relates to penicillium protein elicitor EPTP and application thereof in improving plant disease resistance. The protein sequence of the EPTP protein elicitor is shown as SEQ ID NO.2, and the molecular weight is 19247 Da. EPTP induces significant increase of activity of phenylalanine ammonialyase, peroxidase and polyphenol oxidase, and when the elicitor induces tobacco anaphylactic reaction, an anaphylactic reaction marker gene hin1 is induced to express; it is indicated through semiquantitative RT-PCR results that EPTP can induce tobacco to obtain systemic acquired resistance, cause up-regulation of the tobacco blade resistance gene transcription level, and induce expression of resistance gene related to the salicylic acid path and the jasmonic acid path. After being applied, EPTP can improve resistance to viruses and pathogenic bacteria by plants, takes effects efficiently, does not pollute environment, reduces dosage of chemical pesticide and has wide application prospects as a plant immunity activator.

This invention provides methods whereby taxol, baccatin III, and other taxol-like compounds, or taxanes, can be produced in very high yield from all known Taxus species, e.g., brevifolia, canadensis, cuspidata, baccata, globosa, floridana, wallichiana, media and chinensis. Particular modifications of culture conditions (i.e., media composition and operating modes) have been discovered to enhance the yield of various taxanes from cell culture of all species of Taxus. Particularly preferred enhancement agents include silver ion or complex, jasmonic acid (especially the methyl ester), auxin-related growth regulators, and inhibitors of the phenylpropanoid pathway, such as 3,4-methylenedioxy-6-nitrocinnamic acid. These enhancement agents may be used alone or in combination with one another or other yield-enhancing conditions. While the yield of taxanes from plant cell culture of T. chinensis is particularly enhanced by use of one or more of these conditions, yield of taxanes for all Taxus species has been found to benefit from use of these conditions.

The invention relates to a making method of an aroma type tea, belonging to the technical field of tea-leaf processing. The method comprises the following steps of: spraying 0.05 to 0.65 wt% of jasmonic acid derivative solutions on tea tree leaves, wherein the spraying amount is 40 to 60 L / mu; picking the tea leaves after 20 to 30 hours, and then processing the picked tea leaves according to the making process of a green tea, a black tea or an oolong tea so that the corresponding aroma type tea is obtained. In the making method of the aroma type tea, a jasmonic acid derivative as an external source is applied to process a tea tree, the cell receptors of the tea tree are stimulated to metabolize so that the aroma matter of the tea tree is induced to change, and then the tea tree is processed into the tea leaves in the traditional tea making technology so that the new aroma type tea with high aroma feeling and favorable aroma quality is processed. The invention has simple and easy operation, and can obviously enhance the aroma feeling quality of the tea leaves so that the aroma type tea has favorable aroma quality and durable aroma reservation.

The invention discloses an anti-cracking preservative used before picking of litchis and a preparation method and application thereof. The anti-cracking preservative is prepared from jasmonic acid or jasmonic acid derivative, salicylic acid, chitosan oligosaccharide or / and chitosan, gibberellin, tween 80 or / and sodium dodecyl benzene sulfonate, calcium salt and preservative. The specific application method comprises the following steps: respectively applying the anti-cracking preservative disclosed by the invention in the expansion period, the turning stage and the mature period of the litchis for 3 to 4 times in total once every 7-10 days; uniformly applying the preservative in an atomizing manner to fruits and leaves before 10 a.m. or after 4 p.m. in sunny days until liquid drops fall down; and stopping applying the preservative 7-10 days prior to harvesting. By the application of the anti-cracking preservative, the cracking resistance, cold resistance, disease resistance and storage resistance of the litchis can be improved, and the freshness retention period is prolonged; treated fruits can be stored for more than 8 days under a normal temperature condition and more than 30 days under a low temperature condition; and the cracking rate is low, and is only 50 percent of that of contrast fruits.

The invention relates to a Brassica campestris soilless cultivation method, and belongs to the technical field of soilless cultivation. The Brassica campestris soilless cultivation method includes the following steps: medium preparation, nutriment solution preparation, greenhouse equipment allocation, disc arrangement and soil filling, seed sowing and soil covering, watering and germination, planting preparation, and harvesting. A medium includes perlite, coconut chaff, straw powder, plant residue, saw dust, peanut shells, pig manure, earthworm cast, peptone, silkworm chrysalis meal, conch powder, and broad bean leaf chaff. The nutriment solution includes water, potassium sulphate, urea, sodium molybdate, mild alcohol, indolebutyric acid, gibberellins, ammonium molybdate, zinc sulfate, manganese sulfate, powdered rock phosphate, naphthylacetic acid, saccharose solution, ferment, wood vinegar solution, potassium silicate, jasmonic acid and bacillus subtilis powder. The Brassica campestris soilless cultivation method is rich in medium nutriment, is easy to promote, and is few in pest; and the obtained Brassica campestris is rich in nutriment, is good in taste, and is short in growth period.

This invention provides methods whereby taxol, baccatin III, and other taxol-like compounds, or taxanes, can be produced in very high yield from all known Taxus species, e.g., brevifolia, canadensis, cuspidata, baccata, globosa, floridana, wallichiana, media and chinensis. Particular modifications of culture conditions (i.e., media composition and operating modes) have been discovered to enhance the yield of various taxanes from cell culture of all species of Taxus. Particularly preferred enhancement agents include silver ion or complex, jasmonic acid (especially the methyl ester), auxin-related growth regulators, and inhibitors of the phenylpropanoid pathway, such as 3,4-methylenedioxy-6-nitrocinnamic acid. These enhancement agents may be used alone or in combination with one another or other yield-enhancing conditions. While the yield of taxanes from plant cell culture of T. chinensis is particularly enhanced by use of one or more of these conditions, yield of taxanes for all Taxus species has been found to benefit from use of these conditions.

The invention provides a method for detecting abscisic acid and jasmonic acid in fresh tobacco leaves through liquid chromatogram-tandem mass spectrometry. The method comprises the following steps: (1), preparing an extraction solvent; (2), carrying out liquid nitrogen quick freezing on fresh tobacco leaves, and freezing, drying and smashing; (3), adding the extraction solvent into the well smashed samples, and carrying out low temperature extracting; (4), carrying out refrigerated centrifugation, taking supernate and filtering; (5), carrying out LC-MS / MS detection. The method provided by the invention has the following advantages: a pretreatment method is simple, only ultrasound and filtering are required, and solid phase extraction column purification treatment is not required. Compared with the conventional method, the method provided by the invention adopts LC-MS / MS, the sensibility is high, the accuracy is high, the recovery of standard addition is high, and pretreatment for 100 samples consumes less than 2 hours while methods in existing documents require tedious and time-consuming pretreatments, and about three days are required for 100 samples. Therefore, the method provided by the invention can effectively improve analysis efficiency, and is suitable for analyzing low content objects in a complex matrix.

The invention discloses a detection method of jasmonic acid (JA) and methyl jasmonate (Me Ja) in a trace plant fresh sample on the basis of solid phase extraction and a tandem mass spectrum, comprising the following steps: (1) preparing a reverse carbon eighteen solid phase extraction small column purified and extracted by JA and MeJA; 2) extracting and purifying JA and MeJA in the plant fresh sample with a solid phase extraction method; and (3) sample detection: after the plant sample is purified and extracted, the content of JA and MeJA is detected by LCMS-MS (Liquid Chromatography Mass Spectrometry-Mass Spectrometry). The invention has the effect that the reverse carbon eighteen solid phase extraction small column is suitable for separating and purifying JA and MeJA, and JA and MeJA are simultaneously purified and separated; and meanwhile, JA and MeJA in a complex sample are separated and detected within the shorter time on the basis of the high sensitivity of the tandem mass spectrum to obtain the same result or result better than the prior detection method. The detection method has the advantages of simple result, low sample demand quantity and the like and is convenient to operate so as to detect JA and MeJA in the trace plant fresh sample at high sensitivity.

The present invention is a novel coloring and sugar-increasing fruit ripening agent, which belongs to the agricultural chemical industry and can be widely used as a coloring agent and ripening accelerator for increasing the sugar content of fruits and vegetables. , its formula is as follows (by weight): Potassium dihydrogen phosphate 10%; Anthocyanin 15%; Jasmonolactone 15%; Jasmonic acid 15%; . Instead of artificial pollination and natural pollination, in addition to having a bactericidal effect, it also has a significant effect on increasing production, has no pollution to crops, and has good safety.

The invention relates to a preharvest and postharvest compound treatment method for AP annona squamosa refreshment and fruit cracking restraint. The preharvest and postharvest compound treatment method comprises the steps of enabling complexing agent solution composed of water, calcium chloride and jasmonic acid to be spayed on fruits 1-2 weeks before picking; selecting and harvesting the fruits which are uniform in sizes, have no pest and disease damage and have a maturity of 80-90%; first soaking the harvested fruits in pollution-free preservative solution I composed of water, citric acid, oxalic acid and ascorbic acid for 20-30min, taking out the fruits, and draining moisture; soaking the fruits in preservative solution II onion extracting solution; finally enabling the processed fruits to be aired naturally in an air-conditioning room at 18-20 DEG C, and storing the fruits in a cold storage which is at 15 DEG C and has a relative humidity of 75%-85%; and after storage for 15 days, enabling the fruit cracking rate and the rotting rate of AP annona squamosa to be reduced to be within 15%. According to the preharvest and postharvest compound treatment method, the fruit cracking rate can be effectively reduced, the storage period of the fruits is prolonged, and the flavor and quality of the AP annona squamosa are not affected.

The invention provides a method for improving quality and enhancing aroma of upper leaves of flue-cured tobacco. The method comprises the following steps of: on the basis of conventional ridging fertilization of the flue-cured tobacco, additionally applying 800-1000kg of thoroughly decomposed farmyard manure per mu as base fertilizers; after the middle leaves of the flue-cured tobacco are harvested, evenly applying foliar fertilizers onto the front surfaces and the back surfaces of the upper leaves; and after normal mature harvesting time is delayed for 4-9 days, harvesting the upper leaves in one step and then curing the upper leaves. The foliar fertilizers comprise the following components in percentage by weight: 15-45 percent of borax, 15-45 percent of zinc sulfate, 15-45 percent of manganese sulfate, 3-12 percent of sodium molybdate, 1.5-4.5 percent of copper sulfate and 0-0.001 percent of jasmonic acid. In combination with measures such as additionally applying organic fertilizers in the base fertilizers, applying the foliar fertilizers after the middle leaves are harvested, delaying the harvesting time of the upper leaves and the like, the method for improving quality and enhancing aroma of upper leaves of flue-cured tobacco has the advantages that the method is simple, and the formula use value and the usability of the upper leaves can be greatly improved.

A water-releasing anti-aging composition in the form of an emulsion and process for preparing the anti-aging composition are provided. The anti-aging composition includes an aqueous phase and an oil phase. The aqueous phase includes at least one electrolytic jasmonic acid derivative at a concentration by weight of about 2% to about 20%, based upon weight of the composition. The oil phase includes dimethicone at a concentration by weight of about 1% to about 25%, and an emulsifying crosslinked siloxane elastomer at a concentration by weight of about 0.1% to about 20%, both based upon weight of the composition. The anti-aging composition has a phase ratio of the aqueous phase to the oil phase of about 3.0 to about 12.0. The anti-aging composition converts from an emulsion to a plurality of droplets upon application of shear.

The invention relates to an antibacterial yield-increase composition containing jasmonic acid and methyl jasmonate. The antibacterial yield-increase composition comprises an active component A and an active component B, wherein the active component A comprises g jasmonic acid and methyl jasmonate, and the active component B is any one selected from a group consisting of amino-oligosaccharin, copper humate, naphthylacetic acid and S-abscisic acid. The composition has high activity to a plurality of diseases attacking a plurality of crops, exerts obvious synergistic effect, has a broadened bactericidal spectrum, improves effective fertilizer absorption of crops, promotes good growth of the inferior parts of crops and adjusts the balance of moisture in crops, thereby activating the metabolism system of crops, promotes cell growth of crops and strengthening the root vitality of crops.

The invention discloses a multielement nanometer spray for operating room instrument sterilization and a preparing method thereof. The spray is prepared from propylene glycol, phosphorous acid, nanometer medical stone meal, sodium citrate benzotriazole, fluvastatin, zinc sulfate, iris florentina extract, polygonum orientale extract, aloe-emodin, jasmonic acid, laggera alata extract, 2-phenoxetol, prothioconazole, 4-chloro-3,5-dimethylphenol, iprodione, phosphorous acid, trifloxystrobin, alkyne tert-butyl carbamate, emulgator, defoamer, surfactant, liquid stabilizer, ethanol and deionized water. The spray can achieve antibiosis, lubrication and corrosion resistance, and can be widely applied to operating room instrument sterilization to effectively kill various bacteria and viruses with generating residues and prevent hospital cross infection.