136 results about "Jasmonic acid" patented technology

The invention relates to the technical field of microorganisms and bio-organic fertilizers, and particularly discloses a bio-organic fertilizer for preventing and treating rhizome diseases of horseradish and application of the bio-organic fertilizer, the bio-organic fertilizer comprises the following components: component A: a bacillus velezensis microbial inoculum with the preservation number of CCTCC NO: M 2022923 and a trichoderma yunnanense microbial inoculum with the preservation number of CCTCC NO: M 2025645; the component B is prepared from soybean meal, vinegar residues, charcoal, wollastonite powder and allicin. The biological organic fertilizer disclosed by the invention can be used for increasing the content of growth hormones such as salicylic acid (SA), jasmonic acid (JA) and ethylene (ET) and phytoalexin as well as the activity of chitinase and beta-1, 3-glucanase of a wasabi root system, and has a relatively good prevention and treatment effect on a black heart disease caused by infection of phoma wabi Yokog and a soft rot disease caused by infection of pectobacterium carotovorum subsp. Carotovorum (Pcc). The biological organic fertilizer disclosed by the invention has a relatively good prevention and treatment effect on the soft rot disease caused by infection of the phoma wabi Yokog and a relatively good prevention and treatment effect on the soft rot disease caused by infection of the phoma wabi subsp. Carotovorum (Pcc).

The present invention discloses a method for controlling rice brown planthopper based on sustained-release nano-matrine, belonging to the field of nano-pesticides. In the present invention, matrine is loaded into the mesoporous structure of mesoporous silica, and then encapsulated with chitosan to obtain sustained-release nano-matrine. Then, the sustained-release nano-matrine is formulated into a sustained-release nano-matrine solution, which is then applied as a pesticide to the leaves of rice seedlings to control rice brown planthopper. The sustained-release nano-matrine adopted in the present invention can induce rice seedlings to produce the plant defense hormone jasmonic acid precursor 12-oxophytodienoic acid and the brown planthopper-resistant substances luteolin and cis-aconitine. These substances have a targeted toxic effect and can inhibit the reproduction of offspring, thereby better controlling rice brown planthopper.

The invention discloses application of a transcription factor NtMYC2 as well as an encoding protein and an expression vector thereof in plant epidermal hair regulation and control, and aims to solve the technical problems that a plant epidermal hair generation mechanism is unknown and an effective regulation and control means and a utilization way are lacked at present. A transcription factor NtMYC2 capable of regulating and controlling plant epidermal hair generation is identified, the gene is located in a cell nucleus, the expression level in leaves is the highest, the gene is a key component in a jasmonic acid signal channel, the expression of the gene is regulated and controlled by a jasmonic acid signal, and the gene can be used for regulating and controlling plant epidermal hair generation. By constructing a gene editing strain of the gene, it is clear that NtMYC2 serves as a key gene to participate in positive regulation of epidermal hair generation, and the NtMYC2 has important application value in the aspect of regulating the total epidermal hair development density.

The invention relates to application of a GhRPS20B gene in improvement of insect resistance of cotton bollworm and insect-resistant breeding, and belongs to the technical field of agricultural biology. The invention provides an application of a GhRPS20B gene as shown in SEQ ID NO.1 in improving the disease resistance of cotton bollworm diseases of cotton. Experiments show that the defense mechanism of jasmonic acid (JA) in cotton can be activated by enhancing the transcriptional level of the GhRPS20B gene in a plant body. The GhRPS20B gene is over-expressed in cotton through an agrobacterium tumefaciens-mediated genetic transformation technology, so that the expression level of the GhRPS20B gene in a target plant is enhanced, the plant with enhanced resistance can be effectively cultivated, and the natural defense capability of the plant to cotton bollworms is improved.

The invention belongs to the technical field of biological agents, and particularly relates to ginsenoside variant flavobacterium DG-16 and application thereof in relieving continuous cropping obstacles of angelica sinensis and improving the quality of the angelica sinensis. According to the ginsenoside variant flavobacterium DG-16 disclosed by the invention, the growth of angelica sinensis is promoted by increasing the contents of jasmonic acid and cytokinin hormones, reducing the contents of gibberellin and salicylic acid hormones and promoting the circulation and utilization of nitrogen; in addition, the flavobacterium ginsenoside variant DG-16 can significantly increase the chlorophyll content, is beneficial to photosynthesis of the angelica sinensis, effectively changes the physiological and biochemical states of the angelica sinensis, can resist root rot of the angelica sinensis so as to relieve successive cropping obstacles and improve the quality, and has a significant effect.

The invention discloses a nitrogen fertilizer synergist and application thereof, and belongs to the technical field of fertilizers. The nitrogen fertilizer synergist comprises the following raw materials in percentage by mass: 15-40% of a nitrification inhibitor, 5-10% of butyl dihydrojasmonate, 1-5% of a soil loosening agent, 30-50% of a dispersing aid, 5-10% of an anti-caking agent, 0.1-5% of a trace element fertilizer and 0-5% of nutritional ingredients. According to the nitrogen fertilizer synergist disclosed by the invention, by adding the dihydro jasmonic acid butyl ester, the dispersity of the nitrogen fertilizer synergist can be improved, the fertilizer efficiency of the fertilizer is improved, the growth of plants is promoted, the stress resistance of the plants is improved, the nitrogen fertilizer synergist also has double functions of delaying nitrogen conversion and promoting nitrogen absorption, the utilization efficiency of nitrogen is further improved, and the nitrogen fertilizer synergist is relatively good in flowability and can be applied to the field of agricultural production. The nitrification inhibitor is stable in high-temperature and low-temperature environments, is convenient to use, can be directly used for particle spraying or adding of fertilizer, and solves the problem that nitrification inhibitors such as DMPP can only be used after being dissolved by special solvents.

The invention discloses application of a'microorganism-crop 'interaction signal substance in preparation of a product, the'microorganism-crop' interaction signal substance is acetoin and 2, 3-butanediol, and the product is used for regulating and controlling a soil microbial community, improving abundance of soil phosphate solubilizing bacteria, improving activity of soil alkaline phosphatase and soil respiration intensity, and improving soil quality. The fertilizer can regulate and control metabolic pathways in crops, increase the contents of cane sugar, galactose, melibiose, dehydroascorbic acid, tryptophan, gibberellin, jasmonic acid, methyl jasmonate and ferulic acid in the crops, improve the growth and stress resistance of the crops, promote the growth of the crops, increase the yield of the crops, prevent and control crop diseases and improve the yield of the crops. The soil alkaline phosphatase activity and the microbial respiration intensity are improved. The product is a liquid fungicide, and comprises a bacterial liquid, a microorganism-crop interaction signal substance, and at least one of amino acid, seaweed essence and humic acid. The liquid fungicide can promote crop growth, improve crop stress resistance and effectively inhibit pathogenic bacteria.

The invention discloses endophyte Schizophyllanthus commune CY01-1H for improving synthesis of jasmonic acid and dendrobine of dendrobium nobile seedlings and application of the endophyte Schizophyllanthus commune CY01-1H, and belongs to the technical field of plant secondary metabolism regulation and control and microorganisms. The preservation number of the endophyte CY01-1H is CGMCC (China General Microbiological Culture Collection Center) No.42003; the strain is preserved in China General Microbiological Culture Collection Center on May 12, 2025, and the address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing; the endophytic fungus CY01-1H obtained through separation comes from a healthy dendrobium nobile plant, the content of jasmonic acid of dendrobium nobile and accumulation of dendrobine can be increased by planting the endophytic fungus CY01-1H in dendrobium nobile, the normal growth state of the plant is not affected, and wide application prospects are brought for improving the quality of the dendrobium nobile medicinal material.

The present invention relates to (1) a method for producing biochar having bio stimulating effects, (2) biochar that has been produced to having increased bio stimulating effects, (3) a method for capturing material extracted from biochar and (4) biochar extracts, comprising desired compounds extracted from biochar and suspended in an extract solution. The methods above all involve obtaining a particular biomass source and pyrolyzing the biomass source under specific conditions to increase the bio stimulating effects of the biochar. The method for capturing the material extracted from the biochar produced to have such stimulating effects includes contacting the biochar with a treating liquid, separating the biochar from the biochar extract, collecting the biochar extract from which the biochar has been separated and resuspending the biochar extract in an modified aqueous solution for further use and / or processing separate from, or prior to, disposal. The biochar extract suspended in solution then includes desired compounds (a) at least 0.1 ppm of one or more of the following phenylpropanoid, phenylalanine, flavonoids, sterols, terpenes, terpenoids, tannins, coumarins, benzaldehydes and phenols; (b) at least 0.1 g / L of humics, fulvics or a combination of both humics and fulvics and / or (c) one or more of the following salicylic acid, jasmonic acid, abscisic acid, cytokinin, gibberellic acid and ethylene, by way of example.

The invention provides a method for regulating and controlling aroma of grape fruits through an exogenous plant growth regulator, which comprises the following steps of: spraying three exogenous plant hormones, namely methyl jasmonate, phenylalanine and strigolactones, on 'Hanxiangmi' grape fruits with strong rose aroma, and analyzing the aroma composition and content of the treated grape fruits. The result shows that the rose fragrance intensity of the'Hanxiangmi 'grapes is enhanced after the jasmonic acid methyl ester treatment; after strigolactones treatment, components such as alpha-terpilenol, beta-ionone, nonanoic acid, geraniol and the like in grape fruits are prominent, so that the treated grapes are converted into mixed fragrance type grapes with flower fragrance, fruit fragrance and grass fragrance, and the fragrance layers are rich; and the treatment effect of phenylalanine is not obvious. The invention provides a reference for the research of strigolactone in fruit quality.

The invention belongs to the technical field of selenium enrichment of pakchoi, and particularly relates to a pakchoi growth promoter under selenium-rich and high-cadmium soil conditions. The invention relates to a pakchoi growth promoter under selenium-rich and high-cadmium soil conditions. The pakchoi growth promoter is prepared by uniformly mixing a 1 mmol / L jasmonic acid solution and a 1 mmol / L salicylic acid solution in equal volume and then adding water to dilute for 20 times. The jasmonic acid and the salicylic acid existing in all higher plants are used as raw materials, the optimal formula concentration of the jasmonic acid and the salicylic acid is determined through tests, the synergistic interaction effect of the two hormones is fully exerted, the growth physiological metabolism level of crops in the selenium-rich and high-cadmium soil can be adjusted, the crop yield is increased, and the crop yield is increased. Meanwhile, the content of beneficial element selenium of edible parts of the crops is increased, the content of harmful element cadmium is reduced, healthy and efficient production of the selenium-rich crops is finally achieved, the problems that cadmium exceeds the standard and the yield of the crops is reduced in the utilization process of the selenium-rich and high-cadmium soil are effectively solved, and technical support can be provided for safe utilization of the selenium-rich and high-cadmium soil.

The disclosure relates to methods and compositions for altering the production of one or more secondary plant metabolites comprising applying an effective amount of at least one elicitor, wherein the elicitor is a jasmonate or a salicylate, and combinations thereof. The disclosure further teaches compositions comprising effective amounts of the elicitors disclosed here. The disclosure further relates to methods and compositions for controlling plant pathogens, such as fungal pathogens.

The invention relates to the technical field of biology, in particular to application of protein IbNFYA3 in regulation and control of soft rot resistance of sweet potatoes. It is found for the first time that the content of jasmonic acid and the content of aspartic protease can be remarkably increased through overexpression of the NFYA gene in a plant, so that soft rot bacteria can be effectively resisted, specifically, the invention provides an IbNFYA3 gene for coding an NFYA transcription factor (protein IbNFYA3), the gene is introduced into sweet potatoes to obtain a transgenic sweet potato plant with the overexpression of the IbNFYA3 gene, and the transgenic sweet potato plant has the advantages that the content of jasmonic acid and the content of aspartic protease can be effectively resisted; soft rot resistance identification is carried out on the transgenic sweet potato blocks, and it is found that compared with wild sweet potatoes, the soft rot resistance of the transgenic sweet potato blocks is obviously enhanced. Therefore, the IbNFYA3 protein and the coding gene thereof can regulate and control the soft rot resistance of plants, and can be used for cultivating transgenic plants with high disease resistance.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to a promoter of a pinus massoniana terpene synthase gene Pm TPS (-)-alpha-pinene and application of the promoter. According to the invention, a promoter fragment of a Pm TPS (-)-alpha-pinene gene and three homeopathic action elements with jasmonic acid response on the promoter fragment are obtained from pinus massoniana. A complete promoter fragment of the Pm TPS (-)-alpha-pinene gene of the pinus massoniana is transformed into arabidopsis thaliana by means of agrobacterium mediation, and the promoter can drive expression of a GUS gene in leaves of the arabidopsis thaliana or nicotiana benthamiana. GUS staining and GUS enzyme activity of transgenic Bensi tobacco leaves are reduced along with deletion of jasmonic acid response cis-acting elements on the promoter. Furthermore, the yield of pine resin terpenoids can be increased by utilizing the promoter and the homeopathic element thereof.

The invention belongs to the technical field of pesticides, and particularly relates to a pesticide composition containing trifluorocide, which comprises a component A and a component B. The component A is jasmonic acid; and the component B is trifluorocide. The compound nematicidal composition can significantly improve nematicidal activity, can be processed into missible oil, granules, an emulsion in water, an aqueous solution and other common dosage forms applied in the agricultural field, is used for preventing and treating nematode diseases of vegetable crops, grain crops and fruit trees, has the advantages of low dosage and prominent effect, can delay the problem of drug resistance of existing chemical agents, and can be used for preventing and treating the nematode diseases of the vegetable crops, the grain crops and the fruit trees. The safety requirements of pesticide preparations are met.

The invention relates to a composition for expanding and increasing yield of rhizome crops, a soluble concentrate and application of the soluble concentrate, and belongs to the technical field of plant growth regulators. The composition consists of a prohexadione active substance and a dihydrojasmonic acid active substance, the mass ratio of the prohexadione active substance to the dihydrojasmonic acid active substance is (0.5-25): 1; the prohexadione active substance is prohexadione and / or a soluble salt thereof; the dihydrojasmonic acid active substance is dihydrojasmonic acid and / or soluble salt thereof. The composition is taken as an effective component, a growth regulator is provided, water is taken as a solvent, and auxiliary agents known by technicians in the field are supplemented, so that a soluble concentrate is prepared. The soluble concentrate has the advantages of being environmentally friendly, convenient to process, convenient to use and the like while efficiently achieving swelling and yield increasing effects of root crops, and the problems that special dosage forms need to be prepared in the using process of prohexadione calcium, so that the production cost is high, and use is inconvenient are effectively solved.

The application provides a kind of dihydro jasmonic acid propyl ester soluble powder type that can be compounded with powder fertilizer and a preparation method and application thereof.The powder type includes the following components by weight percentage: dihydro jasmonic acid propyl ester 5-15%, solvent 0-15%, emulsifier 20-50% and filler 35-75%.The application is simple to operate, raw materials are more easily obtained, and mixing and compounding are more convenient.It can be compounded with powder water-soluble fertilizer and high-salinity solution to obtain a mixture, and the mixture can be used in various application scenarios such as foliar spraying, flushing, root irrigation, and root leaching, making it more suitable for large-scale market promotion.It provides more application methods and application scenarios for dihydro jasmonic acid propyl ester and nutrient element compounding to promote fruit quality improvement and shorten the ripening period.

The invention belongs to the technical field of biology, and particularly relates to a gene SbAIF1 for enhancing broad-spectrum disease resistance of sorghum and application of the gene SbAIF1. It is proved for the first time that the SbAIF1 gene is over-expressed in sorghum, the broad-spectrum disease resistance of sorghum is enhanced by increasing the jasmonic acid content in sorghum, a new thought is provided for cultivating disease-resistant transgenic plants, and the SbAIF1 gene has important production significance and life significance for crop breeding and production application.

The invention discloses a method for advancing the fruit maturation period of snowflake pears, and relates to the technical field of fruit and vegetable planting. According to the method, 15 days before fruit harvesting, a fruit ripening promoting agent is sprayed to fruit trees; the fruit maturation promoting agent is any one of 200 [mu] mol / L melatonin, 100 [mu] mol / L jasmonic acid or a mixture of 200 [mu] mol / L melatonin and 100 [mu] mol / L jasmonic acid. According to the method, the arbutin content and the soluble solid content in the early-harvested snowflake pear fruits can be increased, the hardness and the titratable acid content are reduced, and the change of the indexes is synthesized to powerfully prove that the snowflake pears are matured in advance.

The invention discloses application of pseudomonas aeruginosa T17 in prevention and treatment of rice bacterial blight. The invention discloses pseudomonas aeruginosa T17 and a function of a fermentation broth thereof as a biological agent in the aspect of disease control. Experiments prove that the pseudomonas aeruginosa T17 can directly inhibit growth of bacterial blight pathogenic bacteria (Xoo) and pyricularia oryzae (M.oryzae). Meanwhile, the fermentation product filtrate can directly induce up-regulated expression of rice defense related genes and up-regulated expression of jasmonic acid (JA) synthesis key genes OsAOS2, and the resistance of rice to bacterial blight can be enhanced by pretreating rice with the fermentation product filtrate. Therefore, the research lays a foundation for taking the pseudomonas aeruginosa T17 and the fermented filtrate thereof as the rice immune resistance inducer, and provides a new thought for developing innovative pollution-free botanical pesticides.

The application relates to the technical field of pharmaceutical analysis, and discloses a pharmacokinetic analysis method of Tongwei Xiaozheng medicinal liquid, which adopts Q-Orbitrap high-resolution liquid chromatography-mass spectrometry to analyze the concentration of Jasmonic acid in blood components of the Tongwei Xiaozheng medicinal liquid in rat plasma, establishes a plasma concentration determination method for the blood original component Jasmonic acid, carries out methodological verification such as specificity, precision and accuracy, and then calculates pharmacokinetic parameters. In the Tongwei Xiaozheng medicinal liquid, 92 blood components are identified, 17 of which are original components and 75 of which are metabolites, the application firstly determines that Jasmonic acid is one of the blood original components of the Tongwei Xiaozheng medicinal liquid, and reveals the pharmacokinetic characteristics of the Jasmonic acid in normal and model rats. The method has strong specificity, high sensitivity and good reproducibility, and can provide a scientific basis for the research on the pharmacodynamic material basis of the Tongwei Xiaozheng medicinal liquid, the establishment of quality control standards and the clinical rational use of drugs.

The invention belongs to the technical field of plant breeding, and discloses a breeding method for improving the mutagenesis efficiency of stem tips of woody plants. The CRISPR-Cas9 ribonucleoprotein complex and plant hormones 6-benzyladenine, gibberellin and jasmonic acid are co-encapsulated and then accurately delivered to stem tip meristem of woody ornamental plants such as acer truncatum, pteroceltis tatarinowii, crape myrtle and tassel, and the gene mutation obtaining rate of the stem tip meristem is effectively increased; the intelligent environment control system is used for dynamically optimizing illumination, temperature, humidity and oxidative stress, activating a plant endogenous DNA repair pathway and further enhancing homologous recombination efficiency, meanwhile, through collaborative optimization of induction hormone signals and environmental stress, the stress resistance is improved, the leaf color, the plant type and the growth vigor can be accurately regulated and controlled, and the yield of the plant is increased. According to the method, efficient, accurate and stress-resistant linked woody plant stem tip mutation breeding is realized.

The invention discloses an underground propagule composite excitant for ecological restoration and a preparation method thereof, and relates to the technical field of excitant preparation. The fertilizer is prepared from the following raw materials in parts by weight: 0.2 to 0.3 part of gibberellin, 0.2 to 0.5 part of 6-benayl aminopurine, 0.2 to 0.3 part of indolebutyric acid, 0.5 to 0.7 part of sodium nitroprusside, 0.4 to 0.5 part of hydrogen peroxide, 1 to 2 parts of jasmonic acid sustained-release microspheres, 5 to 7 parts of compound amino acid, 1 to 3 parts of compound microbial agent sustained-release microspheres, 2 to 3 parts of seaweed extract, 10 to 13 parts of humic acid, 2 to 3 parts of monopotassium phosphate, 60 to 74 parts of ethanol and 1000 parts of water. The underground propagule composite excitant provided by the invention is beneficial to promoting activation and germination of underground propagules, enhancing plant growth and stress resistance, improving soil micro-ecological functions, forming stable diversity communities and facilitating ecological restoration.

This invention discloses a method for delaying leaf senescence in Paspalum notatum under salt stress. The method includes the following steps: plant material culture and salt stress treatment; H2O2 content determination; DAB staining; chlorophyll content and Fv / Fm value determination; lipidomics data determination and analysis; endogenous hormone level analysis; real-time quantitative PCR analysis of gene expression levels; and transcriptomics data analysis. This invention discovers that root-derived H2O2 in Paspalum notatum under salt-driven conditions delays leaf senescence by participating in the regulation of jasmonic acid. The role of root-derived H2O2 was studied using diphenylthioiodine (DPI), a specific inhibitor of NADPH oxidase. Furthermore, the influence of the presence or absence of root-derived H2O2 on the expression of genes related to jasmonic acid synthesis precursors was investigated at the gene expression level. Mass spectrometry-based omics technology and data analysis are the first key technologies for implementing this project. Lipidomics, as one of the most important branches of metabolomics, can perform qualitative and quantitative analysis at the level of individual lipid species.

The invention relates to an application of a selenium-containing composite initiator as an inhibitor for generating deoxynivalenol in corn, which comprises the following steps: coating corn seeds with the selenium-containing composite initiator to form the corn seeds containing the selenium-containing composite initiator; the selenium-containing composite initiator enhances the resistance of corn to fusarium graminearum and inhibits the synthesis of deoxynivalenol by reprogramming seed metabolism and activating jasmonic acid (JA)-mediated defensive reaction in the seedling stage, and converts phenylpropyl metabolism of seedlings from flavonoid biosynthesis to antifungal phenol biosynthesis. Therefore, seed vigor is synchronized with innate immunity, and an effective strategy is provided for corn crop protection.

The invention provides a bHLH transcription factor OpJRB1 participating in regulation and control of synthesis of ophiorrhiza pumila camptothecin and application of the bHLH transcription factor OpJRB1. An OpJRB1 transcription factor is induced by jasmonic acid, is highly expressed in roots and can interact with inhibitory factors OpJAZ1, OpJAZ5 and OpJAZ6 in a jasmonic acid pathway, which fully shows that the OpJRB1 transcription factor participates in a jasmonic acid signal pathway. Knockout of the OpJRB1 gene significantly reduces the camptothecin content of hairy roots of ophiorrhiza pumila, and overexpression of the OpJRB1 gene can increase the camptothecin content. The OpJRB1 transcription factor can instantly activate the expression of the gene OpTDC1 and the gene OpLAMT1 for synthesizing the camptothecin, so that the accumulation of the camptothecin is improved. The invention provides a novel high-quality medicine source for producing camptothecin with a broad-spectrum anti-cancer curative effect, and has important theoretical significance and potential application value.

This invention provides a pineapple plant growth regulator composition and its regulator, relating to the field of plant growth regulators. The pineapple plant growth regulator composition of this invention comprises the following raw materials in parts by weight: 30-50 parts of calcium diphenylureasulfonate, 15-20 parts of titanium citrate, 8-12 parts of N6-isopentenyladenine, 3-5 parts of 4-iodophenoxyacetic acid, and 10-20 parts of jasmonic acid. This invention uses calcium diphenylureasulfonate, titanium citrate, N6-isopentenyladenine, 4-iodophenoxyacetic acid, and jasmonic acid as core components to prepare a pineapple plant growth regulator composition, which not only significantly increases the single fruit weight of pineapple but also improves fruit quality and effectively reduces the incidence of watercore disease. The plant growth regulator of this invention can enhance the pineapple's defense response against watercore disease, while promoting growth, improving its stress resistance, and increasing fruit quality and yield, resulting in significant benefits in production.

The invention discloses an insecticidal composition containing entomopathogenic virus and jasmonic acid methyl ester or jasmonic acid, and relates to the technical field of biological insecticide, the active ingredients of the insecticidal composition are entomopathogenic virus and jasmonic acid methyl ester or jasmonic acid, and the entomopathogenic virus is nuclear polyhedrosis virus of baculoviridae. The weight ratio of the virus to the jasmonic acid methyl ester or jasmonic acid is 1: (0.5-1000), and the insecticidal composition can be prepared into dosage forms such as a suspending agent, water dispersible granules, wettable powder and the like. The invention also provides a method for preventing and treating lepidoptera pests, which comprises the step of applying the entomopathogenic virus and methyl jasmonate or jasmonic acid to target pests or a living environment of the target pests in a combined manner, and the combined application is simultaneous application or successive interval application. The insecticidal composition has a synergistic effect on lepidoptera pests such as cotton bollworms and beet armyworms, can obviously reduce the food intake, accelerate the death of pests and improve the field control effect, and has a wide application prospect.

The invention discloses application of a BnGAE1 gene in regulation and control of sclerotinia rot resistance of brassica napus, and belongs to the technical field of plant genetic engineering. The gene BnGAE1 capable of enhancing the sclerotinia rot resistance of the brassica napus is separated and cloned from the brassica napus, and the nucleotide sequence of the gene is as shown in SEQ ID NO. 1. Experimental verification shows that the resistance of the BnGAE1 (KO) strain to sclerotinia sclerotiorum is remarkably enhanced, and the incidence rate is reduced by 25.46% after inoculation; physiological and molecular detection shows that the expression quantity of defense related genes (such as PR1 and PDF1.2) in a mutant strain is up-regulated, SA (salicylic acid) and JA / ET (jasmonic acid / ethylene) signal channels are activated, and meanwhile, excessive ROS (reactive oxygen species) is effectively removed, so that the redox balance is maintained. The invention provides a new gene target and a high-efficiency molecular breeding technical path for improvement of sclerotinia rot resistance of colza, and has an important application prospect.

The application provides application of farnesene in prevention and treatment of southern root-knot nematode, belongs to the technical field of prevention and treatment of root-knot nematode, and the concentration of farnesene in prevention and treatment of southern root-knot nematode is 92-118 ng / h per plant. The application finds that farnesene exposure treatment can effectively inhibit the invasion and reproduction of southern root-knot nematode and significantly reduce the feeding preference of southern root-knot nematode to corn by using farnesene to expose and treat corn plants, and farnesene can improve the resistance of plants by regulating and improving the content of defense hormone jasmonic acid isoleucine complex in corn. In conclusion, the application provides a new idea for green prevention and control of southern root-knot nematode.