91 results about "Jasmonic acid" patented technology

The invention belongs to the technical field of biological agents, and particularly relates to ginsenoside variant flavobacterium DG-16 and application thereof in relieving continuous cropping obstacles of angelica sinensis and improving the quality of the angelica sinensis. According to the ginsenoside variant flavobacterium DG-16 disclosed by the invention, the growth of angelica sinensis is promoted by increasing the contents of jasmonic acid and cytokinin hormones, reducing the contents of gibberellin and salicylic acid hormones and promoting the circulation and utilization of nitrogen; in addition, the flavobacterium ginsenoside variant DG-16 can significantly increase the chlorophyll content, is beneficial to photosynthesis of the angelica sinensis, effectively changes the physiological and biochemical states of the angelica sinensis, can resist root rot of the angelica sinensis so as to relieve successive cropping obstacles and improve the quality, and has a significant effect.

The invention discloses endophyte Schizophyllanthus commune CY01-1H for improving synthesis of jasmonic acid and dendrobine of dendrobium nobile seedlings and application of the endophyte Schizophyllanthus commune CY01-1H, and belongs to the technical field of plant secondary metabolism regulation and control and microorganisms. The preservation number of the endophyte CY01-1H is CGMCC (China General Microbiological Culture Collection Center) No.42003; the strain is preserved in China General Microbiological Culture Collection Center on May 12, 2025, and the address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing; the endophytic fungus CY01-1H obtained through separation comes from a healthy dendrobium nobile plant, the content of jasmonic acid of dendrobium nobile and accumulation of dendrobine can be increased by planting the endophytic fungus CY01-1H in dendrobium nobile, the normal growth state of the plant is not affected, and wide application prospects are brought for improving the quality of the dendrobium nobile medicinal material.

The invention provides a method for regulating and controlling aroma of grape fruits through an exogenous plant growth regulator, which comprises the following steps of: spraying three exogenous plant hormones, namely methyl jasmonate, phenylalanine and strigolactones, on 'Hanxiangmi' grape fruits with strong rose aroma, and analyzing the aroma composition and content of the treated grape fruits. The result shows that the rose fragrance intensity of the'Hanxiangmi 'grapes is enhanced after the jasmonic acid methyl ester treatment; after strigolactones treatment, components such as alpha-terpilenol, beta-ionone, nonanoic acid, geraniol and the like in grape fruits are prominent, so that the treated grapes are converted into mixed fragrance type grapes with flower fragrance, fruit fragrance and grass fragrance, and the fragrance layers are rich; and the treatment effect of phenylalanine is not obvious. The invention provides a reference for the research of strigolactone in fruit quality.

The disclosure relates to methods and compositions for altering the production of one or more secondary plant metabolites comprising applying an effective amount of at least one elicitor, wherein the elicitor is a jasmonate or a salicylate, and combinations thereof. The disclosure further teaches compositions comprising effective amounts of the elicitors disclosed here. The disclosure further relates to methods and compositions for controlling plant pathogens, such as fungal pathogens.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to a promoter of a pinus massoniana terpene synthase gene Pm TPS (-)-alpha-pinene and application of the promoter. According to the invention, a promoter fragment of a Pm TPS (-)-alpha-pinene gene and three homeopathic action elements with jasmonic acid response on the promoter fragment are obtained from pinus massoniana. A complete promoter fragment of the Pm TPS (-)-alpha-pinene gene of the pinus massoniana is transformed into arabidopsis thaliana by means of agrobacterium mediation, and the promoter can drive expression of a GUS gene in leaves of the arabidopsis thaliana or nicotiana benthamiana. GUS staining and GUS enzyme activity of transgenic Bensi tobacco leaves are reduced along with deletion of jasmonic acid response cis-acting elements on the promoter. Furthermore, the yield of pine resin terpenoids can be increased by utilizing the promoter and the homeopathic element thereof.

The invention relates to a composition for expanding and increasing yield of rhizome crops, a soluble concentrate and application of the soluble concentrate, and belongs to the technical field of plant growth regulators. The composition consists of a prohexadione active substance and a dihydrojasmonic acid active substance, the mass ratio of the prohexadione active substance to the dihydrojasmonic acid active substance is (0.5-25): 1; the prohexadione active substance is prohexadione and / or a soluble salt thereof; the dihydrojasmonic acid active substance is dihydrojasmonic acid and / or soluble salt thereof. The composition is taken as an effective component, a growth regulator is provided, water is taken as a solvent, and auxiliary agents known by technicians in the field are supplemented, so that a soluble concentrate is prepared. The soluble concentrate has the advantages of being environmentally friendly, convenient to process, convenient to use and the like while efficiently achieving swelling and yield increasing effects of root crops, and the problems that special dosage forms need to be prepared in the using process of prohexadione calcium, so that the production cost is high, and use is inconvenient are effectively solved.

The application provides a kind of dihydro jasmonic acid propyl ester soluble powder type that can be compounded with powder fertilizer and a preparation method and application thereof.The powder type includes the following components by weight percentage: dihydro jasmonic acid propyl ester 5-15%, solvent 0-15%, emulsifier 20-50% and filler 35-75%.The application is simple to operate, raw materials are more easily obtained, and mixing and compounding are more convenient.It can be compounded with powder water-soluble fertilizer and high-salinity solution to obtain a mixture, and the mixture can be used in various application scenarios such as foliar spraying, flushing, root irrigation, and root leaching, making it more suitable for large-scale market promotion.It provides more application methods and application scenarios for dihydro jasmonic acid propyl ester and nutrient element compounding to promote fruit quality improvement and shorten the ripening period.

The invention belongs to the technical field of biology, and particularly relates to a gene SbAIF1 for enhancing broad-spectrum disease resistance of sorghum and application of the gene SbAIF1. It is proved for the first time that the SbAIF1 gene is over-expressed in sorghum, the broad-spectrum disease resistance of sorghum is enhanced by increasing the jasmonic acid content in sorghum, a new thought is provided for cultivating disease-resistant transgenic plants, and the SbAIF1 gene has important production significance and life significance for crop breeding and production application.

The invention discloses a method for advancing the fruit maturation period of snowflake pears, and relates to the technical field of fruit and vegetable planting. According to the method, 15 days before fruit harvesting, a fruit ripening promoting agent is sprayed to fruit trees; the fruit maturation promoting agent is any one of 200 [mu] mol / L melatonin, 100 [mu] mol / L jasmonic acid or a mixture of 200 [mu] mol / L melatonin and 100 [mu] mol / L jasmonic acid. According to the method, the arbutin content and the soluble solid content in the early-harvested snowflake pear fruits can be increased, the hardness and the titratable acid content are reduced, and the change of the indexes is synthesized to powerfully prove that the snowflake pears are matured in advance.

The invention discloses application of pseudomonas aeruginosa T17 in prevention and treatment of rice bacterial blight. The invention discloses pseudomonas aeruginosa T17 and a function of a fermentation broth thereof as a biological agent in the aspect of disease control. Experiments prove that the pseudomonas aeruginosa T17 can directly inhibit growth of bacterial blight pathogenic bacteria (Xoo) and pyricularia oryzae (M.oryzae). Meanwhile, the fermentation product filtrate can directly induce up-regulated expression of rice defense related genes and up-regulated expression of jasmonic acid (JA) synthesis key genes OsAOS2, and the resistance of rice to bacterial blight can be enhanced by pretreating rice with the fermentation product filtrate. Therefore, the research lays a foundation for taking the pseudomonas aeruginosa T17 and the fermented filtrate thereof as the rice immune resistance inducer, and provides a new thought for developing innovative pollution-free botanical pesticides.

The application relates to the technical field of pharmaceutical analysis, and discloses a pharmacokinetic analysis method of Tongwei Xiaozheng medicinal liquid, which adopts Q-Orbitrap high-resolution liquid chromatography-mass spectrometry to analyze the concentration of Jasmonic acid in blood components of the Tongwei Xiaozheng medicinal liquid in rat plasma, establishes a plasma concentration determination method for the blood original component Jasmonic acid, carries out methodological verification such as specificity, precision and accuracy, and then calculates pharmacokinetic parameters. In the Tongwei Xiaozheng medicinal liquid, 92 blood components are identified, 17 of which are original components and 75 of which are metabolites, the application firstly determines that Jasmonic acid is one of the blood original components of the Tongwei Xiaozheng medicinal liquid, and reveals the pharmacokinetic characteristics of the Jasmonic acid in normal and model rats. The method has strong specificity, high sensitivity and good reproducibility, and can provide a scientific basis for the research on the pharmacodynamic material basis of the Tongwei Xiaozheng medicinal liquid, the establishment of quality control standards and the clinical rational use of drugs.

The invention belongs to the technical field of plant breeding, and discloses a breeding method for improving the mutagenesis efficiency of stem tips of woody plants. The CRISPR-Cas9 ribonucleoprotein complex and plant hormones 6-benzyladenine, gibberellin and jasmonic acid are co-encapsulated and then accurately delivered to stem tip meristem of woody ornamental plants such as acer truncatum, pteroceltis tatarinowii, crape myrtle and tassel, and the gene mutation obtaining rate of the stem tip meristem is effectively increased; the intelligent environment control system is used for dynamically optimizing illumination, temperature, humidity and oxidative stress, activating a plant endogenous DNA repair pathway and further enhancing homologous recombination efficiency, meanwhile, through collaborative optimization of induction hormone signals and environmental stress, the stress resistance is improved, the leaf color, the plant type and the growth vigor can be accurately regulated and controlled, and the yield of the plant is increased. According to the method, efficient, accurate and stress-resistant linked woody plant stem tip mutation breeding is realized.

The invention discloses an underground propagule composite excitant for ecological restoration and a preparation method thereof, and relates to the technical field of excitant preparation. The fertilizer is prepared from the following raw materials in parts by weight: 0.2 to 0.3 part of gibberellin, 0.2 to 0.5 part of 6-benayl aminopurine, 0.2 to 0.3 part of indolebutyric acid, 0.5 to 0.7 part of sodium nitroprusside, 0.4 to 0.5 part of hydrogen peroxide, 1 to 2 parts of jasmonic acid sustained-release microspheres, 5 to 7 parts of compound amino acid, 1 to 3 parts of compound microbial agent sustained-release microspheres, 2 to 3 parts of seaweed extract, 10 to 13 parts of humic acid, 2 to 3 parts of monopotassium phosphate, 60 to 74 parts of ethanol and 1000 parts of water. The underground propagule composite excitant provided by the invention is beneficial to promoting activation and germination of underground propagules, enhancing plant growth and stress resistance, improving soil micro-ecological functions, forming stable diversity communities and facilitating ecological restoration.

This invention discloses a method for delaying leaf senescence in Paspalum notatum under salt stress. The method includes the following steps: plant material culture and salt stress treatment; H2O2 content determination; DAB staining; chlorophyll content and Fv / Fm value determination; lipidomics data determination and analysis; endogenous hormone level analysis; real-time quantitative PCR analysis of gene expression levels; and transcriptomics data analysis. This invention discovers that root-derived H2O2 in Paspalum notatum under salt-driven conditions delays leaf senescence by participating in the regulation of jasmonic acid. The role of root-derived H2O2 was studied using diphenylthioiodine (DPI), a specific inhibitor of NADPH oxidase. Furthermore, the influence of the presence or absence of root-derived H2O2 on the expression of genes related to jasmonic acid synthesis precursors was investigated at the gene expression level. Mass spectrometry-based omics technology and data analysis are the first key technologies for implementing this project. Lipidomics, as one of the most important branches of metabolomics, can perform qualitative and quantitative analysis at the level of individual lipid species.

The invention relates to an application of a selenium-containing composite initiator as an inhibitor for generating deoxynivalenol in corn, which comprises the following steps: coating corn seeds with the selenium-containing composite initiator to form the corn seeds containing the selenium-containing composite initiator; the selenium-containing composite initiator enhances the resistance of corn to fusarium graminearum and inhibits the synthesis of deoxynivalenol by reprogramming seed metabolism and activating jasmonic acid (JA)-mediated defensive reaction in the seedling stage, and converts phenylpropyl metabolism of seedlings from flavonoid biosynthesis to antifungal phenol biosynthesis. Therefore, seed vigor is synchronized with innate immunity, and an effective strategy is provided for corn crop protection.

The invention provides a bHLH transcription factor OpJRB1 participating in regulation and control of synthesis of ophiorrhiza pumila camptothecin and application of the bHLH transcription factor OpJRB1. An OpJRB1 transcription factor is induced by jasmonic acid, is highly expressed in roots and can interact with inhibitory factors OpJAZ1, OpJAZ5 and OpJAZ6 in a jasmonic acid pathway, which fully shows that the OpJRB1 transcription factor participates in a jasmonic acid signal pathway. Knockout of the OpJRB1 gene significantly reduces the camptothecin content of hairy roots of ophiorrhiza pumila, and overexpression of the OpJRB1 gene can increase the camptothecin content. The OpJRB1 transcription factor can instantly activate the expression of the gene OpTDC1 and the gene OpLAMT1 for synthesizing the camptothecin, so that the accumulation of the camptothecin is improved. The invention provides a novel high-quality medicine source for producing camptothecin with a broad-spectrum anti-cancer curative effect, and has important theoretical significance and potential application value.

This invention provides a pineapple plant growth regulator composition and its regulator, relating to the field of plant growth regulators. The pineapple plant growth regulator composition of this invention comprises the following raw materials in parts by weight: 30-50 parts of calcium diphenylureasulfonate, 15-20 parts of titanium citrate, 8-12 parts of N6-isopentenyladenine, 3-5 parts of 4-iodophenoxyacetic acid, and 10-20 parts of jasmonic acid. This invention uses calcium diphenylureasulfonate, titanium citrate, N6-isopentenyladenine, 4-iodophenoxyacetic acid, and jasmonic acid as core components to prepare a pineapple plant growth regulator composition, which not only significantly increases the single fruit weight of pineapple but also improves fruit quality and effectively reduces the incidence of watercore disease. The plant growth regulator of this invention can enhance the pineapple's defense response against watercore disease, while promoting growth, improving its stress resistance, and increasing fruit quality and yield, resulting in significant benefits in production.

The invention discloses an insecticidal composition containing entomopathogenic virus and jasmonic acid methyl ester or jasmonic acid, and relates to the technical field of biological insecticide, the active ingredients of the insecticidal composition are entomopathogenic virus and jasmonic acid methyl ester or jasmonic acid, and the entomopathogenic virus is nuclear polyhedrosis virus of baculoviridae. The weight ratio of the virus to the jasmonic acid methyl ester or jasmonic acid is 1: (0.5-1000), and the insecticidal composition can be prepared into dosage forms such as a suspending agent, water dispersible granules, wettable powder and the like. The invention also provides a method for preventing and treating lepidoptera pests, which comprises the step of applying the entomopathogenic virus and methyl jasmonate or jasmonic acid to target pests or a living environment of the target pests in a combined manner, and the combined application is simultaneous application or successive interval application. The insecticidal composition has a synergistic effect on lepidoptera pests such as cotton bollworms and beet armyworms, can obviously reduce the food intake, accelerate the death of pests and improve the field control effect, and has a wide application prospect.

The invention discloses application of a BnGAE1 gene in regulation and control of sclerotinia rot resistance of brassica napus, and belongs to the technical field of plant genetic engineering. The gene BnGAE1 capable of enhancing the sclerotinia rot resistance of the brassica napus is separated and cloned from the brassica napus, and the nucleotide sequence of the gene is as shown in SEQ ID NO. 1. Experimental verification shows that the resistance of the BnGAE1 (KO) strain to sclerotinia sclerotiorum is remarkably enhanced, and the incidence rate is reduced by 25.46% after inoculation; physiological and molecular detection shows that the expression quantity of defense related genes (such as PR1 and PDF1.2) in a mutant strain is up-regulated, SA (salicylic acid) and JA / ET (jasmonic acid / ethylene) signal channels are activated, and meanwhile, excessive ROS (reactive oxygen species) is effectively removed, so that the redox balance is maintained. The invention provides a new gene target and a high-efficiency molecular breeding technical path for improvement of sclerotinia rot resistance of colza, and has an important application prospect.

The application provides application of farnesene in prevention and treatment of southern root-knot nematode, belongs to the technical field of prevention and treatment of root-knot nematode, and the concentration of farnesene in prevention and treatment of southern root-knot nematode is 92-118 ng / h per plant. The application finds that farnesene exposure treatment can effectively inhibit the invasion and reproduction of southern root-knot nematode and significantly reduce the feeding preference of southern root-knot nematode to corn by using farnesene to expose and treat corn plants, and farnesene can improve the resistance of plants by regulating and improving the content of defense hormone jasmonic acid isoleucine complex in corn. In conclusion, the application provides a new idea for green prevention and control of southern root-knot nematode.

The application provides a method for constructing a regulation network of a potato JA pathway in response to Cd stress. The method relates to the technical field of data processing, and comprises the following steps: obtaining multi-omics time series data of potatoes under the conditions of cadmium stress and methyl jasmonate treatment; performing signal decomposition and feature purification on the multi-omics time series data by using a weak stationary signal decomposition method based on sequence patterns to obtain a plurality of intrinsic mode components; constructing a dynamic regulation network model to capture multi-scale change patterns of response characteristics in the time dimension and the concentration dimension, thereby extracting deep features; inputting the intrinsic mode components into the trained dynamic regulation network model to output an attention weight matrix, thereby forming a regulation network representing the dynamic regulation intensity of the jasmonic acid pathway; and solving the optimal exogenous methyl jasmonate concentration for promoting biomass and inhibiting cadmium accumulation by optimizing an objective function. The application provides a high-precision model tool for analyzing the regulation mechanism of plant hormone signals under heavy metal stress.

The invention discloses a method for enhancing pine wood nematode disease resistance of larch through jasmonic acid signal mediated flavonoid biosynthesis. The method comprises the following steps that 1, the larch is inoculated with pine wood nematode; 2, performing high-throughput omics sequencing sampling and data analysis; 3, treating larch seedlings with methyl jasmonate; 4, the inhibition effect of the kaempferol and the citrus aurantium on the pine wood nematodes is obtained. According to the method, larch is taken as a research object, a disease stress system is constructed by artificially inoculating pine wood nematodes, leaf / stem tissues at different time points after infection are selected, and sequential multiomics analysis is carried out by combining transcriptome sequencing and a wide targeting metabolome technology; the theoretical basis and method for improving the pine wood nematode disease resistance of larch by regulating and controlling the biosynthesis of kaempferol and citrus aurantium through a jasmonic acid signal way are defined.

Methods and compositions to produce insect resistance in plants deploy an engineered glutamate receptor-like protein (GLR3.3) that functions in transmitting wound-induced signals from local to systemic plant organs to trigger synthesis of jasmonic acid, a defense hormone.

The present invention relates to the technical field of microorganisms and bio-organic fertilizers, and specifically discloses a bio-organic fertilizer for preventing and treating wasabi root diseases and its application. The bio-organic fertilizer comprises the following components: Component A: Bacillus velezensis agent with a deposit number of CCTCC NO: M 2022923 and Trichoderma yunnanensis agent with a deposit number of CCTCC NO: M 2025645; Component B: soybean meal, vinegar dregs, biochar, wollastonite powder, and allicin. The bio-organic fertilizer of the present invention can increase the content of growth hormones such as salicylic acid (SA), jasmonic acid (JA), and ethylene (ET), and the phytoalexin content in the wasabi root system, as well as the activity of chitinase and β-1,3-glucanase, and has an effect on the growth of stem mold of wasabi ( Phoma wasabiae Yokog) infection and carrot soft rot caused by Pectobacterium carrot subspecies ( Pectobacterium carotovorum subsp .carotovorum, It has a good control effect on soft rot caused by Pcc) infection.

This invention relates to the field of microbial technology, and particularly to a peanut-specific growth regulator and its application in sugarcane-peanut intercropping. The peanut-specific growth regulator of this invention consists of 1 g / L-3 g / L of GX-2-sugarcane leaf microbial growth regulator, 2 g / L-6 g / L of GX-7-peanut vine microbial growth regulator, 0.2 g / L-0.4 g / L of brassinolide, and 0.2 g / L-0.4 g / L of jasmonic acid. At this concentration, the peanut-specific growth regulator can significantly increase peanut yield. Under sugarcane-peanut intercropping conditions, the yield increase is even more significant. This peanut-specific growth regulator can provide a new approach for the development of specialized agricultural biological agents.

This invention belongs to the field of bio-agricultural technology, specifically relating to genes. DISC1 The application of its encoded proteins in regulating plant resistance to Phytophthora parasiticans. This invention, through the study of... DISC1 Studies on the function of genes and their encoded proteins have revealed that... DISC1 Negative regulation of plant resistance to Phytophthora parasiticum. Overexpressed genes. DISC1 Significantly reduces plant resistance to Phytophthora parasiticum, gene DISC1 The mutant plants exhibited significantly enhanced resistance to Phytophthora parasiticum. Further research into the immune mechanism revealed that the gene... DISC1 This invention regulates plant immune responses by modulating genes related to the jasmonic acid (JA) signaling pathway. It provides a novel gene resource for the control of plant resistance to Phytophthora parasiticum.

The invention provides a plant insect-resistant related protein PtoJAZ10 and application thereof, and belongs to the technical field of molecular biology. The endogenous insect-resistant gene PtoJAZ10 of the poplar is cloned, and an overexpression and gene editing vector of the endogenous insect-resistant gene PtoJAZ10 is constructed, so that the gene is proved in a woody plant to remarkably enhance the insect-resistant capability of the plant by regulating a jasmonic acid signal channel for the first time; the overexpression plant shows the core advantages that the leaf loss rate is reduced, and the development of pests is inhibited; meanwhile, antioxidant enzyme (SOD / POD / CAT) and defensive enzyme (PPO / PAL) are activated to synergistically respond to insect attack stress; the insect resistance of the plant with deletion of gene editing is completely lost, and the functional necessity of the PtoJAZ10 is reversely verified. The achievement breaks through the limitation that the traditional insect-resistant gene depends on exogenous species, and provides a new efficient and safe genetic breeding strategy for cultivating a new endogenous insect-resistant poplar variety.

This invention discloses a method for cultivating enoki mushrooms using camellia peel as a culture medium, belonging to the field of enoki mushroom cultivation technology. The method includes the following steps: (1) Pretreatment: crushing and sieving camellia peel to obtain camellia peel residue; (2) Degradation treatment: pre-wetting camellia peel residue with water, adding mixed inoculant, wheat bran, sucrose, quicklime, and degradation agent, and fermenting to obtain culture medium; (3) Substrate treatment: adding jasmonic acid to the culture medium, treating it under high temperature and high pressure, and then drying it to obtain the treated culture medium; (4) Mushroom bag making: packing the treated culture medium into bags, inoculating enoki mushroom spawn under aseptic conditions to obtain mushroom bags; (5) Mushroom cultivation: placing the mushroom bags in a cultivation room for mycelial cultivation, and managing mushroom cultivation after the mycelium has fully grown; (6) Harvesting: generally, the stipe can be harvested when it reaches 13-14cm in length and the cap diameter is 0.8-1.5cm.

The present invention addresses the problem of providing a method with which plants that are not currently used for food, or parts of said plants, are made edible. The present invention also addresses the problem of providing a method for improving the taste of plants that are used for food, or parts of said plants. Provided is a crop or a food / beverage that is made of a plant in which the expression and / or activity of a jasmonic acid defense-related protein is suppressed or inhibited, or part of said plant, or that contains any one thereof, the crop being an edible crop or a feed crop for animals excluding Agromyzidae, and the food / beverage being for humans or being feed for animals excluding Agromyzidae.

The invention relates to the technical field of fertilizers, in particular to application of a composition containing jasmonic acid to improvement of root rot resistance of southern rhodiola roots. The jasmonic acid-containing composition prepared by the invention has a remarkable effect on improving the root rot resistance of the southern rhododendron. Experimental results show that after the plant is treated by the compound, the resistance of the plant to fusarium oxysporum infection is obviously enhanced, the disease time is delayed, and the disease infection degree is obviously reduced. Field application further proves that the treatment can effectively improve the survival rate of the plants and reduce the susceptibility rate, has no adverse effect on the yield of the plants, and shows good agronomic applicability.