2 results about "Baccatin III" patented technology

Disclosed are a method for biological total synthesis of paclitaxel key precursor substance baccatin III, a biological material and use thereof. The bioenzyme composition used in the method comprises a taxane 9α-hydroxylase (T9αH) and a taxane C4-C20 oxetanase (TOT). According to the disclosure, a core component of a baccatin III biosynthetic pathway is identified, a baccatin III artificial synthesis route is constructed, thereby paving the way for efficiently developing a green low-carbon production pathway of taxane products (such as paclitaxel) through synthetic biology.

The invention discloses a nucleotide fragment combination for constructing a paclitaxel biosynthesis engineering strain and an engineering strain construction method, and relates to the technical field of bioengineering, the nucleotide fragment combination comprises a nucleotide fragment I, a nucleotide fragment II and a nucleotide fragment III; each fragment respectively comprises genes required by the biological synthesis pathway of 5a-hydroxylated taxadiene, baccatin III and paclitaxel; in each nucleotide fragment, a single promoter is at most used for starting four genes, and the genes started by the single promoter are connected through 2A peptides. The invention also discloses a method for constructing the paclitaxel biosynthesis engineering strain and the paclitaxel biosynthesis engineering strain. According to the invention, the nucleotide fragment combination is utilized to successfully construct the taxol biosynthesis engineering strain of the taxiphocephalum leptosphaeroides through iterative transformation, so that stable biosynthesis of the taxol in a heterologous chassis is realized, and the method has important practical application value.