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Raoultella ornithinolytica and applications thereof

A technology of bacteria strains and microbial strains, applied in the field of microbiology

Active Publication Date: 2015-06-24
TIANJIN UNIV OF SCI & TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Raoultella strains have not been found to be capable of converting baccatin III to 10-DAB

Method used

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  • Raoultella ornithinolytica and applications thereof
  • Raoultella ornithinolytica and applications thereof
  • Raoultella ornithinolytica and applications thereof

Examples

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Embodiment Construction

[0038]The present invention will be further described in detail below through the specific examples, the following examples are only descriptive, not restrictive, and cannot limit the protection scope of the present invention with this.

[0039] Content of the present invention includes following content:

[0040] 1. By putting the sludge from the sludge concentration room of Tianjin Taida Wastewater Treatment Plant into a single-chamber microbial fuel cell, the biofilm enriched on the anode observed by the naked eye was taken as the research object, and separated and purified by cultivable methods Pure species of microorganisms with electrochemical activity are obtained.

[0041] 2. Analyze the electricity production performance of isolate P1-A-19 by running MFCs.

[0042] 3. The ability of isolate P1-A-19 to transform baccatin III into 10-DAB was studied by TLC, HPLC and LC-MS.

[0043] The specific discussion is as follows:

[0044] 1. The main steps of isolation, screen...

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Abstract

The invention discloses a raoultella ornithinolytica P1-A-19, which is prepared by carrying out enrichment, separation, purification and screening on sludge produced by sewage treatment plants. The raoultella ornithinolytica P1-A-19 is characterized in that the strain shows an electricity-production capacity when applied to a microbial fuel cell, and also has a capacity of converting baccatin III so as to generate 10-deacetyl baccatin III (10-DAB), and the Preservation Number of the strain is CGMCC No. 10267. The raoultella ornithinolytica P1-A-19 disclosed by the invention has a great significance for the enrichment of diversity of electricity-production microorganisms and the excavation of more microbial strains with high electrochemical activity; and microbial strains for 10-DAB biosynthesis are further widened, thereby providing new materials and new methods for the development of new taxane compound synthesis approaches.

Description

technical field [0001] The invention belongs to the field of microbes, and relates to a new bacterial strain Raoultine ornithinolyticum capable of producing electricity and capable of transforming baccatin III into 10-DAB and an application thereof. Background technique [0002] 1. Electrogenic microorganisms [0003] Microbial Fuel Cells (MFCs) is a new type of device that uses microorganisms as a catalyst to oxidize organic matter and directly convert chemical energy into electrical energy. It combines fuel cells and biological technology to achieve direct biochemical energy and electrical energy. Transformation, which is a clean and sustainable energy, has gradually become the focus of new energy development. [0004] Electric-producing microorganisms (Electricigens) refer to those that can completely oxidize organic matter under anaerobic conditions, transfer the electrons obtained by oxidizing organic matter to the outside of the cell through the electron transport cha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12Q1/04H01M8/16C12P17/02C12R1/01
CPCC12N1/20C12P17/02C12N1/205C12R2001/01H01M8/16Y02E60/50Y02P70/50
Inventor 骆健美朱凤芝周明华王敏
Owner TIANJIN UNIV OF SCI & TECH
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