190 results about "Microbiological strains" patented technology

Platforms, systems, and methods for genetic generalization in synthetic biology development. According to one aspect, there is provided a method for predicting performance associated with genetic edits, the method comprising: receiving, by a platform, information about a strain of a microorganism, wherein the information about the strain comprises information describing a plurality of genetic edits to a base strain of the microorganism; generating, by the platform, a set of genetic embeddings based on the information about the strain, wherein the generating comprises processing the information about the strain using one or more embedding models, wherein each of the one or more embedding models: receives the information about the strain of the microorganism as input; and applies computational transformations to the input using a corresponding embedding model to generate a multi-dimensional vector representation for each of the plurality of genetic edits.

The present invention provides a long-period high-pressure enrichment culture and online monitoring apparatus and method for deep-sea microorganisms. The apparatus can simulate extreme deep-sea environments such as high pressure, low temperature, and oligotrophic conditions. Through pre-culture and graded serial culture, stable long-period growth and reproduction of the deep-sea microorganisms are ensured. An online system is used for monitoring the abundance, activity, and culture conditions of the microorganisms in real time to determine an appropriate timing for strain subculturing, and timely interactive subculturing and culture are performed, thereby achieving stable supply of high-abundance, high-activity microorganism enrichment culture solution. The present invention achieves precise pressure-retaining abundance counting of the microorganisms under in-situ pressure conditions and accurate control of a microbial growth cycle during enrichment culture, effectively improving timeliness and culture efficiency of the microorganism enrichment culture solution, and enabling long-period acquisition of high-abundance, high-activity microbial strain liquid.

The invention relates to a multifunctional pseudomonas Y2-2 and application thereof, the strain is a multifunctional microbial strain, can effectively utilize microorganisms to solve the problem of heavy metal environmental pollution remediation, can also restrain various plant diseases and insect pests, and is classified and named as Pseudomonas sp. The strain is preserved in China General Microbiological Culture Collection Center (CGMCC) on April 21, 2025, the address is Institute of Microbiology, Chinese Academy of Sciences, No.3, No.1 Yard, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC No.34287. The method can effectively utilize microorganisms to repair heavy metal environmental pollution, can inhibit various plant diseases and insect pests, is good in effect and high in efficiency, and has practical popularization and application values.

The invention relates to a large-scale high-yield and high-efficiency cultivation mode of pumpkins in saline-alkali soil, which adopts a composite modifier to improve the saline-alkali soil, and the composite modifier contains an organic fertilizer, a soil conditioner and a saline-alkali tolerant microbial strain in a specific proportion, so that the soil structure and fertility are effectively improved; a pumpkin variety with a salt-tolerant gene marker is selected, and the salt tolerance and the survival rate of seedlings are remarkably improved by combining a biochar seedling raising technology; through an intelligent drip irrigation system and a precise fertilization technology, the irrigation and fertilization amount is dynamically adjusted according to the growth stage of pumpkins and soil fertility, and the resource utilization efficiency is improved; a disease and pest control method combining physical and biological control is adopted, meanwhile, an S-shaped vine pressing method is combined to promote plant growth, and product quality tracing is achieved through a block chain technology. The problem of pumpkin planting in the saline-alkali soil is effectively solved, the yield and quality of pumpkins are remarkably improved, remarkable economic benefits and ecological benefits are achieved, and a new thought and method are provided for agricultural development of the saline-alkali soil.

The invention relates to the field of microbial strains edited by gene recombination and synthetic biology, and provides recombinant escherichia coli for synthesizing D-xylose by using glucose and application. The recombinant escherichia coli comprises the following modifications: exogenous insertion of a gene IRX for coding beta-1, 4-xylosyltransferase; exogenous insertion of a gene UXS encoding a UDP-glucuronic acid decarboxylase; the invention relates to enhanced expression or overexpression of a gene yagH for coding beta xylosidase. Compared with a traditional hemicellulose hydrolysis method for producing D-xylose, the method for synthesizing and preparing D-xylose through whole-cell catalysis of D-glucose by using the recombinant escherichia coli has the advantages of environmental protection, cost advantage and energy saving.

According to the AI-assisted marine microorganism anti-tumor activity product separation method provided by the invention, a microorganism strain with high anti-tumor activity potential can be directly and quickly screened out by constructing a marine microorganism sample database and applying a trained HetGNN model; the workload and time cost of tedious fermentation culture and biological activity testing in a traditional screening process are greatly reduced, meanwhile, the screening period is shortened, and the screening efficiency is greatly improved. Besides, a closed-loop feedback system formed by the invention feeds back a high-purity target compound sample separated from a laboratory and related data thereof to a microorganism sample database, and continuously optimizes the integrity of the database and the performance of the HetGNN model, so that the accuracy and efficiency of subsequent anti-tumor active compound screening are remarkably improved; the problem that in the prior art, screening precision is limited by initial data, and iterative upgrading is difficult is solved.

The invention discloses bacillus megaterium and application thereof in prevention and treatment of cucumber downy mildew. The bacillus megaterium ZF521 for preventing and treating the cucumber downy mildew, provided by the invention, has the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.31036 in the General Microbiological Culture Collection Center of the China Committee for Culture Collection of Microorganisms. A potted living body test shows that the highest prevention and treatment effect of the bacillus megaterium on the cucumber downy mildew can reach 72.62%, meanwhile, the bacillus megaterium CGMCC No.31036 can promote the increase of the content of three endogenous hormones in the cucumber, and the bacillus megaterium CGMCC No.31036 is applied to the cucumber, so that the cucumber downy mildew can be effectively prevented and treated, and the growth of the cucumber is promoted.

This invention relates to a microbial agent with highly efficient plastic degradation properties and its application, wherein the microbial agent contains *Cryptococcus lauriensis* strain with accession number CCTCC NO: M 2026232. Papiliotrema laurentii SNP7-2P strain and / or Achromobacterium with accession number CCTCC NO: M 2026231 Achromobacter strains FJ1-2L. These two microbial strains exhibit varying degrees of degradation effects on long-chain polymers in plastics, effectively and environmentally addressing plastic pollution and making them suitable for preparing green and environmentally friendly degradable plastic products.

The invention relates to the technical field of biological fermentation, in particular to a method for improving the fermentation stability of vitamin B12, which comprises the following steps: acquiring a microbial strain of vitamin B12 and activating; preparing a culture medium suitable for fermentation of vitamin B12 according to a composite carbon source system, an optimized nitrogen source combination, key elements, other nutritional ingredients, plant three elements and a modified buffer agent; according to the fermentation stages, the fermentation conditions are accurately controlled stage by stage; an online monitoring system is established, key parameters in the fermentation process are monitored in real time, a neural network model is established, and the stability and high efficiency of the fermentation process are ensured. According to the method for improving the fermentation stability of the vitamin B12, a diversified carbohydrate mixture is used as a carbon source, a nitrogen source combination is optimized, key elements are accurately added, a specific buffer system is constructed, and an online monitoring system and a neural network model are established for big data analysis and intelligent prediction; the synthesis of vitamin B12 is promoted, and the stability and high efficiency of the fermentation process are ensured.

The microorganism strain Pediococcus acidilactici TAK 589 CoccobEst is a new antimicrobial and antioxidant probiotic, CoccobEst inhibits the growth of the enteropathogens L. monocytogenes, E. coli, S. enteritidis, S. typhimurium, S. sonnei, S. aureus, E. faecalis and C. sakazakii, but does not inhibit lactobacilli of natural intestinal microbiota. The strain's antimicrobial properties are enhanced by periplasmic peptidoglycan hydrolases with proteolytic activity. CoccobEst is used as a zootechnieai feed additive to prevent bacterial infections in the gastrointestinal tract of farm animals and pets and to prevent and reduce diarrhea. CoccobEst has highly active antioxidant defense system, increasing the redox activity of glutathione and reducing oxidative stress in cells. Therefore, CoccobEst is used as a food supplement or functional food for humans to prevent bacterial infections of the gastrointestinal tract and to prevent and reduce oxidative stress. In addition, CoccobEst is used as a starter culture in fermentation of vegetables.

The invention relates to the field of microorganisms, and discloses a stress-resistant heterotrophic nitrification-aerobic denitrification nitrogen removal bacterium and application thereof. The pseudomonas is named as H1, the microorganism classification name of the pseudomonas is Pseudomonas sihuiensis, the preservation number of the pseudomonas is CGMCC (China General Microbiological Culture Collection Center) NO: 33481, the preservation date of the pseudomonas is January 20, 2025, and the preservation place of the pseudomonas is China General Microbiological Culture Collection Center. The pseudomonas H1 disclosed by the invention can still realize efficient denitrification in a heterotrophic nitrification-aerobic denitrification way in a high-salt and high-nitrogen water body environment containing toxic pollutants with benzene ring functional groups, so that the pseudomonas H1 has great application potential in biological treatment of nitrogen-containing wastewater.

The present invention relates to microbial compositions, useful as biological amendments to enhance plant growth. Provided are isolated microbial strains, agricultural compositions comprising them, and their use in methods of improving plant growth and / or increasing plant yield.

The present invention relates to microorganisms for improving human and animal health. More particularly, the present invention relates to isolated Bacillus strains, as well as strains having all identification characteristics of these strains, for use including the above-mentioned use, in particular for improving gastrointestinal health in animals or humans.

This invention provides a method, apparatus, device, and medium for predicting the yield of metabolites, relating to the field of data processing technology. The method includes: acquiring a feature set of a target strain; the feature set is constructed based on all k-mer sequences of the target strain that are positively correlated with and negatively correlated with the target metabolite; inputting the feature set into a prediction model to obtain the predicted yield of the target metabolite output by the prediction model. By constructing a prediction model that directly correlates the k-mer sequences of the genomic low-level sequence with the yield of the metabolite, direct prediction from genotype to phenotype is achieved. This approach avoids the time-consuming and laborious experimental screening process of traditional methods, providing an efficient and accurate computational tool for high-throughput screening and targeted modification of microbial strains, shortening the metabolite prediction cycle, and achieving a rapid and accurate prediction process for the yield of metabolites.

The invention provides a pulullan refining method, which belongs to the technical field of biology, and specifically comprises the following steps: fermenting a microbial strain for producing pulullan to obtain fermentation liquor, and sterilizing and decoloring the fermentation liquor to obtain a crude pure extracting solution; carrying out a membrane process and a resin process on the obtained crude pure extracting solution to obtain a refined pure solution; the pore diameter of the membrane material is 500-1000Da; according to the method disclosed by the invention, it is found for the first time that the pullulan is extracted and purified by using a small-aperture nanofiltration membrane and is matched with resin adsorption, so that the purification effect can be achieved, the purity of the product is improved, and the yield of the product is also obviously improved.

The disclosure relates to genetically modified microorganisms of the genus Bacillus, for the improvement of phenotypes of plants, for example nitrogen availability for non-leguminous plants. Included are novel strains of the microorganisms, microbial consortia, and agricultural compositions comprising the same. Furthermore, the disclosure teaches methods of utilizing the described microorganisms, microbial consortia, and agricultural compositions comprising the same, in methods for imparting beneficial properties to target plant species. In particular aspects, the disclosure provides methods of increasing desirable plant traits in agronomically important species, for example nitrogen fixation, utilization, regulation, uptake, acquisition, tolerance, and / or processing in plants.

The invention discloses a strain specific culture medium prediction method, a medium and computer equipment. The method comprises the following steps: acquiring strain gene function annotation data and encoding the data into a high-dimensional feature vector; performing principal component analysis dimension reduction on the high-dimensional feature vector; obtaining candidate culture medium component information and encoding the candidate culture medium component information into a binary vector; splicing the low-dimensional strain feature representation and the medium component vector to form a joint input feature vector; inputting the joint input feature vector into a pre-trained deep residual network model; and outputting a growth compatibility probability score and sorting the candidate culture media. By integrating high-throughput genome annotation data and a known culture medium formula, a data-driven intelligent prediction model is constructed and is used for efficiently recommending an optimal culture medium composition suitable for a specific microbial strain, and the prediction precision and the screening speed of microbial isolated culture are remarkably improved.

The application discloses a cadmium-resistant proteus mirabilis and application thereof in treatment of composite pollution environment. Priestia megaterium The application provides a PBAT-degrading bacterium, i.e., a proteus mirabilis (S261-3) strain, which can grow at a temperature of 10-50 DEG C and has certain salt and cadmium resistance, and can grow normally in a high-salt or high-cadmium concentration range. Meanwhile, the S261-3 strain has a good PBAT-degrading effect, and can degrade PBAT under cadmium stress, and can be used for degrading plastic pollutants in cadmium-polluted soil. The microbial strain provided by the application has stronger adaptability to a polluted environment, meets the repair demand of a composite pollution site, can be used for comprehensive treatment of microplastic and heavy metal composite pollution, is helpful to improving soil quality and improving land utilization efficiency, and has important practical significance for soil composite pollution repair.

This invention relates to the field of microbial technology, specifically to a Pseudomonas bacterium with broad-spectrum pesticide degradation properties and its applications. Pseudomonas bacterium (… Pseudomonas japonica The accession number of strain 11-9 is GDMCC NO: 64579. This invention obtained microbial strain 11-9, which has both plant growth-promoting characteristics and broad-spectrum pesticide degradation function, through isolation and screening. It has degradation effects on different types of pesticides. Compared with single pesticide-degrading bacteria, this strain is more suitable for farmland soil remediation under complex pollution conditions.

The application relates to the technical field of microbial strain preservation, in particular to a microbial strain preservation device which comprises a freezer, a moving assembly and a rotating assembly, the freezer is provided with a box door, a fixing seat is arranged in the freezer, the end of the fixing seat is provided with a supporting disc, a plurality of groups of test tube racks are fixedly connected to the supporting disc, and the bottom end of the supporting plate is fixedly connected with a connecting shaft; the moving assembly is arranged in the freezer and is used for driving the supporting disc to displace; and the rotating assembly is used for driving the connecting shaft to rotate, so that the supporting disc rotates. The moving assembly and the rotating assembly are arranged, the connecting shaft drives the supporting plate to move a small distance through the action of the moving assembly, part of the position of the supporting disc is moved to the outside of the freezer, then the connecting shaft drives the supporting disc to rotate through the cooperation of the rotating assembly, and then the strain at any position on the supporting plate can be taken and placed, so that the operation is more convenient.

The present invention relates to an improved cysteine-producing strain, in particular to a microbial strain suitable for the fermentative production of L-cysteine, characterized in that the relative enzymatic activity of the enzymes identified by the number EC 2.7. 9.2 in the KEGG database is inactivated or the specific activity is reduced based on the wild-type enzymes, and the enzyme represented by the number EC 2.7. 9.2 in the KEGG database forms an increased amount of L-cysteine as compared to a microbial strain having a wild-type enzymatic activity in which the gene encoding the enzymatic activity is represented by ppsA. The invention also provides methods of producing L-cysteine using these microbial cells.

Methods and uses of compositions (e.g. comprising one or more microbial strains, one or more components, one or more metabolites, or a combination thereof) for treating insulin-associated diseases, disorders, and conditions are disclosed.

The present application belongs to the technical field of biological control, and particularly relates to a microbial agent for preventing and treating gray mold of crops, and a preparation method and application thereof. The present application provides a microbial agent for preventing and treating gray mold of crops, which comprises a microbial strain and / or a fermentation broth of the microbial strain; the microbial strain comprises one or more of Bacillus subtilis JD-L9, Pseudomonas lundensis DS-PZ-P18 and Pseudomonas chlororaphis HQ-CZ-P12; wherein the Pseudomonas chlororaphis HQ-CZ-P12, the Pseudomonas lundensis DS-PZ-P18 and the Bacillus subtilis JD-L9 have a good inhibition rate on the pathogenic fungus of gray mold, and after being compounded into a compound microbial agent, the compound microbial agent is sprayed on seedlings of medicinal materials, so that the incidence of gray mold during seedling raising is significantly reduced, pesticide residues of the medicinal materials are reduced, and the safety of the medicinal materials is improved.

The invention belongs to the field of synthetic biology and metabolic engineering, and discloses a screening method and application of a microbial strain with high yield of ergothioneine, a promoter (such as PECL1, PBSC5, PEDC2 and the like) responding to ergothioneine is screened through transcriptomics analysis based on saccharomyces cerevisiae, and the promoter can present high sensitivity and linear response under 0-1000 mg / L of ergothioneine. The strain is used for driving fluorescent protein gene expression, a fluorescence report strain is constructed, the strain can rapidly and sensitively detect the concentration of ergothioneine in fermentation liquor by virtue of fluorescence signal change, and high-yield strains are efficiently screened. The method overcomes the problems of low flux, long period, low sensitivity and the like of traditional screening, and the fluorescence signal intensity is in significant linear correlation with the ergothioneine concentration. And the perforated plate and the microplate reader are used for automatic high-throughput screening, so that the efficiency is improved, the resource consumption is reduced, effective support is provided for production optimization of ergothioneine, and the application prospect is wide.

The invention discloses an application method of a microbial compound fertilizer for desert grassland, and belongs to the technical field of agricultural fertilization. Aiming at the problem that the application effect of the existing microbial fertilizer in severe environments such as desert grassland is unstable, the method is solved by the following scheme: firstly, collecting a soil sample for physical and chemical analysis, selecting an adaptive microbial strain based on an analysis result, and preparing into a complex microbial inoculant; the microbial agent and a bentonite carrier are mixed to prepare a microbial compound fertilizer, and the fertilization depth and dosage are determined by adopting a grading adaptation process according to specific obstacle indexes (such as total petroleum hydrocarbon content) of soil; performing preliminary regulation and control on soil humidity, temperature and pH after fertilization; and finally, establishing a dynamic field management strategy based on fertilization effect feedback, and dynamically calculating and adjusting appropriate target values of humidity and temperature by regularly monitoring key index changes of soil, so that the soil microenvironment is continuously optimized according to the microbial fertilizer efficiency exertion condition, and precise fertilization and soil improvement are realized.

The invention discloses a compound microorganism feed additive for promoting the growth of livestock and poultry. The compound microorganism feed additive comprises microorganism strains, natural plant components and auxiliary materials, the microbial strains comprise 8 to 12 parts of candida utilis, 5 to 9 parts of bacillus licheniformis, 2 to 4 parts of bacillus subtilis, 8 to 12 parts of aspergillus niger, 8 to 12 parts of lactobacillus delbrueckii, 8 to 12 parts of lactobacillus acidophilus, 8 to 12 parts of bifidobacterium and 3 to 7 parts of lactobacillus salivarius; the natural plant components comprise 0.5 to 2 parts of moringa seed powder; the auxiliary materials comprise 20 to 30 parts of cane sugar, 10 to 15 parts of fish meal, 2 to 3 parts of grease, 10 to 20 parts of soybean meal, 5 to 10 parts of pupa powder, 10 to 15 parts of zinc lactate, 1 to 2 parts of calcium gluconate, 3 to 5 parts of calcium carbonate and 2 to 4 parts of paeonol. Microbial strains in the compound microbial feed additive can generate various growth regulatory factors such as vitamins, amino acids and organic acids, so that the growth and development of livestock and poultry are stimulated, and the growth speed is increased.

The present invention provides for a vessel comprising: (a) electrodes, in electrical communication with a power source, and (b) a solution comprising a microbial strain capable of synthesizing a compound of interest, wherein the microbial strain obtains energy via water-splitting electrolysis through the electrodes and microbial hydrogen fermentation.

The invention relates to the technical field of food microorganisms, in particular to agile combined lactobacillus and application thereof. The invention provides an agile combined lactobacillus with the preservation number of CGMCC (China General Microbiological Culture Collection Center) NO.33722. The agile combined lactobacillus has the advantages that the preservation number is CGMCC NO.33722; according to the invention, the lactic acid bacteria capable of promoting rapid propagation and having a modification effect on soybean protein are separated from the soybean whey water, and the lactic acid bacteria can adapt to relatively high culture temperature and is high in propagation speed. The lactic acid bacterium agent prepared by utilizing the microbial strain can be applied to the production process of the soybean protein, can be used for replacing hydrochloric acid and improving the dispersity of the soybean protein, and has a wide application prospect.

The invention relates to a compound bacterium, a meat fermentation composition and a meat fermentation method, the compound bacterium is used for meat fermentation, and the compound bacterium comprises at least one of the following three bacteria: lactobacillus plantarum XC-1-3 preserved in the China General Microbiological Culture Collection Center (CGMCC) with the preservation number of CGMCC No.36530, lactobacillus plantarum XC-1-3 preserved in the China General Microbiological Culture Collection Center (CGMCC), lactobacillus plantarum XC-1-3 preserved in the China General Microbiological Culture Collection Center (CGMCC), and lactobacillus plantarum XC-1-3 preserved in the China General Microbiological Culture Collection Center (CGMCC). The lactobacillus plantarum YC-1-4 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number of the lactobacillus plantarum YC-1-4 is CGMCC No.36529, the lactobacillus plantarum MXC-4 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number of the lactobacillus plantarum MXC-4 is CGMCC No.36528, and the lactobacillus plantarum MXC-4 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number of the lactobacillus plantarum MXC-4 is CGMCC No.36528. In the synergistic fermentation, the compound bacteria can effectively improve the flavor and quality of the fermented meat, the vinasse liquid can promote the high-value utilization of vinasse byproducts, and the synergistic effect of the compound bacteria and the vinasse liquid realizes the dual improvement of the flavor and the shelf life of the fermented meat product.