119 results about "Microbiological strains" patented technology

Platforms, systems, and methods for genetic generalization in synthetic biology development. According to one aspect, there is provided a method for predicting performance associated with genetic edits, the method comprising: receiving, by a platform, information about a strain of a microorganism, wherein the information about the strain comprises information describing a plurality of genetic edits to a base strain of the microorganism; generating, by the platform, a set of genetic embeddings based on the information about the strain, wherein the generating comprises processing the information about the strain using one or more embedding models, wherein each of the one or more embedding models: receives the information about the strain of the microorganism as input; and applies computational transformations to the input using a corresponding embedding model to generate a multi-dimensional vector representation for each of the plurality of genetic edits.

The present invention provides a long-period high-pressure enrichment culture and online monitoring apparatus and method for deep-sea microorganisms. The apparatus can simulate extreme deep-sea environments such as high pressure, low temperature, and oligotrophic conditions. Through pre-culture and graded serial culture, stable long-period growth and reproduction of the deep-sea microorganisms are ensured. An online system is used for monitoring the abundance, activity, and culture conditions of the microorganisms in real time to determine an appropriate timing for strain subculturing, and timely interactive subculturing and culture are performed, thereby achieving stable supply of high-abundance, high-activity microorganism enrichment culture solution. The present invention achieves precise pressure-retaining abundance counting of the microorganisms under in-situ pressure conditions and accurate control of a microbial growth cycle during enrichment culture, effectively improving timeliness and culture efficiency of the microorganism enrichment culture solution, and enabling long-period acquisition of high-abundance, high-activity microbial strain liquid.

The invention discloses bacillus megaterium and application thereof in prevention and treatment of cucumber downy mildew. The bacillus megaterium ZF521 for preventing and treating the cucumber downy mildew, provided by the invention, has the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.31036 in the General Microbiological Culture Collection Center of the China Committee for Culture Collection of Microorganisms. A potted living body test shows that the highest prevention and treatment effect of the bacillus megaterium on the cucumber downy mildew can reach 72.62%, meanwhile, the bacillus megaterium CGMCC No.31036 can promote the increase of the content of three endogenous hormones in the cucumber, and the bacillus megaterium CGMCC No.31036 is applied to the cucumber, so that the cucumber downy mildew can be effectively prevented and treated, and the growth of the cucumber is promoted.

This invention relates to a microbial agent with highly efficient plastic degradation properties and its application, wherein the microbial agent contains *Cryptococcus lauriensis* strain with accession number CCTCC NO: M 2026232. Papiliotrema laurentii SNP7-2P strain and / or Achromobacterium with accession number CCTCC NO: M 2026231 Achromobacter strains FJ1-2L. These two microbial strains exhibit varying degrees of degradation effects on long-chain polymers in plastics, effectively and environmentally addressing plastic pollution and making them suitable for preparing green and environmentally friendly degradable plastic products.

The invention provides a pulullan refining method, which belongs to the technical field of biology, and specifically comprises the following steps: fermenting a microbial strain for producing pulullan to obtain fermentation liquor, and sterilizing and decoloring the fermentation liquor to obtain a crude pure extracting solution; carrying out a membrane process and a resin process on the obtained crude pure extracting solution to obtain a refined pure solution; the pore diameter of the membrane material is 500-1000Da; according to the method disclosed by the invention, it is found for the first time that the pullulan is extracted and purified by using a small-aperture nanofiltration membrane and is matched with resin adsorption, so that the purification effect can be achieved, the purity of the product is improved, and the yield of the product is also obviously improved.

The invention discloses a strain specific culture medium prediction method, a medium and computer equipment. The method comprises the following steps: acquiring strain gene function annotation data and encoding the data into a high-dimensional feature vector; performing principal component analysis dimension reduction on the high-dimensional feature vector; obtaining candidate culture medium component information and encoding the candidate culture medium component information into a binary vector; splicing the low-dimensional strain feature representation and the medium component vector to form a joint input feature vector; inputting the joint input feature vector into a pre-trained deep residual network model; and outputting a growth compatibility probability score and sorting the candidate culture media. By integrating high-throughput genome annotation data and a known culture medium formula, a data-driven intelligent prediction model is constructed and is used for efficiently recommending an optimal culture medium composition suitable for a specific microbial strain, and the prediction precision and the screening speed of microbial isolated culture are remarkably improved.

The application discloses a cadmium-resistant proteus mirabilis and application thereof in treatment of composite pollution environment. Priestia megaterium The application provides a PBAT-degrading bacterium, i.e., a proteus mirabilis (S261-3) strain, which can grow at a temperature of 10-50 DEG C and has certain salt and cadmium resistance, and can grow normally in a high-salt or high-cadmium concentration range. Meanwhile, the S261-3 strain has a good PBAT-degrading effect, and can degrade PBAT under cadmium stress, and can be used for degrading plastic pollutants in cadmium-polluted soil. The microbial strain provided by the application has stronger adaptability to a polluted environment, meets the repair demand of a composite pollution site, can be used for comprehensive treatment of microplastic and heavy metal composite pollution, is helpful to improving soil quality and improving land utilization efficiency, and has important practical significance for soil composite pollution repair.

This invention relates to the field of microbial technology, specifically to a Pseudomonas bacterium with broad-spectrum pesticide degradation properties and its applications. Pseudomonas bacterium (… Pseudomonas japonica The accession number of strain 11-9 is GDMCC NO: 64579. This invention obtained microbial strain 11-9, which has both plant growth-promoting characteristics and broad-spectrum pesticide degradation function, through isolation and screening. It has degradation effects on different types of pesticides. Compared with single pesticide-degrading bacteria, this strain is more suitable for farmland soil remediation under complex pollution conditions.

The application relates to the technical field of microbial strain preservation, in particular to a microbial strain preservation device which comprises a freezer, a moving assembly and a rotating assembly, the freezer is provided with a box door, a fixing seat is arranged in the freezer, the end of the fixing seat is provided with a supporting disc, a plurality of groups of test tube racks are fixedly connected to the supporting disc, and the bottom end of the supporting plate is fixedly connected with a connecting shaft; the moving assembly is arranged in the freezer and is used for driving the supporting disc to displace; and the rotating assembly is used for driving the connecting shaft to rotate, so that the supporting disc rotates. The moving assembly and the rotating assembly are arranged, the connecting shaft drives the supporting plate to move a small distance through the action of the moving assembly, part of the position of the supporting disc is moved to the outside of the freezer, then the connecting shaft drives the supporting disc to rotate through the cooperation of the rotating assembly, and then the strain at any position on the supporting plate can be taken and placed, so that the operation is more convenient.

The present invention relates to an improved cysteine-producing strain, in particular to a microbial strain suitable for the fermentative production of L-cysteine, characterized in that the relative enzymatic activity of the enzymes identified by the number EC 2.7. 9.2 in the KEGG database is inactivated or the specific activity is reduced based on the wild-type enzymes, and the enzyme represented by the number EC 2.7. 9.2 in the KEGG database forms an increased amount of L-cysteine as compared to a microbial strain having a wild-type enzymatic activity in which the gene encoding the enzymatic activity is represented by ppsA. The invention also provides methods of producing L-cysteine using these microbial cells.

Methods and uses of compositions (e.g. comprising one or more microbial strains, one or more components, one or more metabolites, or a combination thereof) for treating insulin-associated diseases, disorders, and conditions are disclosed.

The present application belongs to the technical field of biological control, and particularly relates to a microbial agent for preventing and treating gray mold of crops, and a preparation method and application thereof. The present application provides a microbial agent for preventing and treating gray mold of crops, which comprises a microbial strain and / or a fermentation broth of the microbial strain; the microbial strain comprises one or more of Bacillus subtilis JD-L9, Pseudomonas lundensis DS-PZ-P18 and Pseudomonas chlororaphis HQ-CZ-P12; wherein the Pseudomonas chlororaphis HQ-CZ-P12, the Pseudomonas lundensis DS-PZ-P18 and the Bacillus subtilis JD-L9 have a good inhibition rate on the pathogenic fungus of gray mold, and after being compounded into a compound microbial agent, the compound microbial agent is sprayed on seedlings of medicinal materials, so that the incidence of gray mold during seedling raising is significantly reduced, pesticide residues of the medicinal materials are reduced, and the safety of the medicinal materials is improved.

The invention belongs to the field of synthetic biology and metabolic engineering, and discloses a screening method and application of a microbial strain with high yield of ergothioneine, a promoter (such as PECL1, PBSC5, PEDC2 and the like) responding to ergothioneine is screened through transcriptomics analysis based on saccharomyces cerevisiae, and the promoter can present high sensitivity and linear response under 0-1000 mg / L of ergothioneine. The strain is used for driving fluorescent protein gene expression, a fluorescence report strain is constructed, the strain can rapidly and sensitively detect the concentration of ergothioneine in fermentation liquor by virtue of fluorescence signal change, and high-yield strains are efficiently screened. The method overcomes the problems of low flux, long period, low sensitivity and the like of traditional screening, and the fluorescence signal intensity is in significant linear correlation with the ergothioneine concentration. And the perforated plate and the microplate reader are used for automatic high-throughput screening, so that the efficiency is improved, the resource consumption is reduced, effective support is provided for production optimization of ergothioneine, and the application prospect is wide.

The invention discloses an application method of a microbial compound fertilizer for desert grassland, and belongs to the technical field of agricultural fertilization. Aiming at the problem that the application effect of the existing microbial fertilizer in severe environments such as desert grassland is unstable, the method is solved by the following scheme: firstly, collecting a soil sample for physical and chemical analysis, selecting an adaptive microbial strain based on an analysis result, and preparing into a complex microbial inoculant; the microbial agent and a bentonite carrier are mixed to prepare a microbial compound fertilizer, and the fertilization depth and dosage are determined by adopting a grading adaptation process according to specific obstacle indexes (such as total petroleum hydrocarbon content) of soil; performing preliminary regulation and control on soil humidity, temperature and pH after fertilization; and finally, establishing a dynamic field management strategy based on fertilization effect feedback, and dynamically calculating and adjusting appropriate target values of humidity and temperature by regularly monitoring key index changes of soil, so that the soil microenvironment is continuously optimized according to the microbial fertilizer efficiency exertion condition, and precise fertilization and soil improvement are realized.

The invention relates to the technical field of food microorganisms, in particular to agile combined lactobacillus and application thereof. The invention provides an agile combined lactobacillus with the preservation number of CGMCC (China General Microbiological Culture Collection Center) NO.33722. The agile combined lactobacillus has the advantages that the preservation number is CGMCC NO.33722; according to the invention, the lactic acid bacteria capable of promoting rapid propagation and having a modification effect on soybean protein are separated from the soybean whey water, and the lactic acid bacteria can adapt to relatively high culture temperature and is high in propagation speed. The lactic acid bacterium agent prepared by utilizing the microbial strain can be applied to the production process of the soybean protein, can be used for replacing hydrochloric acid and improving the dispersity of the soybean protein, and has a wide application prospect.

The invention relates to a compound bacterium, a meat fermentation composition and a meat fermentation method, the compound bacterium is used for meat fermentation, and the compound bacterium comprises at least one of the following three bacteria: lactobacillus plantarum XC-1-3 preserved in the China General Microbiological Culture Collection Center (CGMCC) with the preservation number of CGMCC No.36530, lactobacillus plantarum XC-1-3 preserved in the China General Microbiological Culture Collection Center (CGMCC), lactobacillus plantarum XC-1-3 preserved in the China General Microbiological Culture Collection Center (CGMCC), and lactobacillus plantarum XC-1-3 preserved in the China General Microbiological Culture Collection Center (CGMCC). The lactobacillus plantarum YC-1-4 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number of the lactobacillus plantarum YC-1-4 is CGMCC No.36529, the lactobacillus plantarum MXC-4 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number of the lactobacillus plantarum MXC-4 is CGMCC No.36528, and the lactobacillus plantarum MXC-4 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number of the lactobacillus plantarum MXC-4 is CGMCC No.36528. In the synergistic fermentation, the compound bacteria can effectively improve the flavor and quality of the fermented meat, the vinasse liquid can promote the high-value utilization of vinasse byproducts, and the synergistic effect of the compound bacteria and the vinasse liquid realizes the dual improvement of the flavor and the shelf life of the fermented meat product.

The invention relates to the technical field of microbial cultivation, in particular to a self-adaptive microbial evolution system based on a PLC (Programmable Logic Controller). The system comprises at least one culture module, a pressure applying module, an online monitoring module, an automatic operation module and an intelligent control unit. According to the invention, by integrating the microreactor array, the on-line multi-parameter monitoring system and the PLC, a closed-loop automatic platform integrating functions of microorganism enrichment, directional domestication and high-throughput screening is constructed; the invention aims to effectively solve the problems of large manual operation proportion, insufficient environmental control precision, poor experimental repeatability and the like in the traditional microorganism enrichment, domestication and screening method, and can be applied to the fields of environmental bioremediation, industrial biological manufacturing, agricultural microbial preparation development and the like which need to efficiently obtain high-performance microbial strains.

The invention discloses a dry farmland saline-alkali soil treatment method by matching microbial agent spraying with organic fertilizer application, and belongs to the technical field of saline-alkali soil treatment. According to the method, specific microbial strains are screened to prepare a microbial agent, and the microbial agent is combined with application of an organic fertilizer, so that the soil structure of the dry farmland saline-alkali soil is improved, the soil salinity and alkalinity are reduced, and the soil fertility is improved, and effective treatment and improvement of the dry farmland saline-alkali soil are realized. Experimental data show that after the treatment method is adopted, the pH value of the soil is remarkably reduced, the organic matter content and the available nutrient content of the soil are remarkably increased, the crop yield is also greatly increased, and good application prospects are achieved.

The invention belongs to the technical field of natural product extraction and fermentation engineering, and particularly relates to a tobacco fermentation liquor extract with acetylcholin esterase inhibition and antioxidant activity as well as a preparation method and application of the tobacco fermentation liquor extract. Specifically, research finds that by taking tobacco leaves or tobacco waste as a substrate and screening a nicotine-resistant microbial strain with high fermentability for co-culture fermentation, acetylcholin esterase inhibition and antioxidant activity of metabolite from a single plant or microorganism source can be effectively improved; therefore, the tobacco fermentation liquor extract with acetylcholin esterase inhibition and antioxidant activity is obtained. Tobacco leaves or tobacco wastes are used as raw materials to prepare products with biomedical potential, and a new way is provided for promoting multipurpose utilization of tobaccos.

The disclosure provides a microbial community strain identification method and system based on genetic pattern design, and relates to the technical field of microbial strain identification, comprising: obtaining a strain gene sequence and preprocessing it; arranging the preprocessed strain gene sequence according to the genome sequence to obtain the gene arrangement result of each strain; designing a discriminative genetic pattern based on the gene arrangement result, solving the minimal specific substring in a set of genome sequences to obtain the specific genetic pattern of each strain, and defining the combined specific genetic pattern based on combinatorial mathematics; based on the specific genetic pattern and the combined specific genetic pattern, constructing a quadratic programming model to estimate the strain abundance through the alignment between metagenomic sequencing reads and genetic patterns, and identifying the optimal composition of the strain abundance in the strain gene sequence sample. The disclosure realizes accurate analysis of the strain composition and its relative abundance in the sample.

The present disclosure relates to a genetically modified cell capable of utilizing sucrose as energy and carbon source following the expression of a single heterologous enzyme, which upon expression is translocated from the cytosol and which is capable of hydrolysing non-phosphorylated sucrose into fructose and glucose.The identification of efficient enzymes capable of hydrolysing sucrose in its non-modified form, and which on its own enable the cell to utilize sucrose as a, or as the main and / or sole, carbon and / or energy source, i.e., without the need for multi-gene sucrose utilizing systems comprising several other heterologous polypeptides, such as other enzymes and / or transporters, is highly advantageous, since it allows for cost-effective use of sucrose in large scale production processes.

The invention relates to a recombinant microbial strain for de novo synthesis of gardenin B and application of the recombinant microbial strain in production of polymethoxylated flavone compounds, and belongs to a cross technology of synthetic biology and organic synthetic chemistry. The invention designs and constructs a heterozygous gardenin B biosynthesis pathway, and obtains a microbial strain for synthesizing gardenin B from the beginning. By screening F8H and redox partner types and regulating and controlling subcellular localization, an F8H-redox partner combination with optimal space and type is determined, and an engineering strain of which the gardenin B yield is greatly improved is obtained. The efficient synthesis method of the polymethoxylated flavonoids is established by selecting recombinant microorganisms for synthesizing gardenin B from de novo to perform co-culture fermentation or chemical conversion of fermentation products.

The invention discloses cadmium-resistant P.megaterium and an application of the cadmium-resistant P.megaterium in treatment of a combined polluted environment. The invention provides a PBAT (poly (butyleneadipate-co-terephthalate)) efficient degradation bacterium, namely, a Pristia megaterium S261-3 strain, and the strain can grow at the temperature of 10-50 DEG C, has certain salt and cadmium resistance, and can normally grow in a relatively high salt or cadmium concentration range. Meanwhile, the S261-3 strain has a good degradation effect on PBAT, can also degrade PBAT under cadmium stress, and can be used for degrading plastic pollutants in cadmium-polluted soil. The microbial strain provided by the invention has stronger adaptability to polluted environment, meets the repair requirement of a combined pollution site, can be used for comprehensive treatment of micro-plastic and heavy metal combined pollution, is beneficial to improvement of soil quality and improvement of land utilization efficiency, and has important practical significance for soil combined pollution repair.

The present invention relates to a microbiological typing method comprising providing: a database of genetic profiles of microbial strains; and a phylogenetic tree, assigning the genetic spectrum to a location in the phylogenetic tree, and assigning a frequency of variation of the spectrum in the tree. The microbial typing comprises the following steps: measuring genetic spectrums of the microorganisms, determining the variation probability of each genetic spectrum in a database relative to the measured genetic spectrums, and calculating the variation probability according to the variation frequency; and determining that the microorganism belongs to a classification unit of the tree if at least the probability of the classification unit is above a threshold. According to the invention, the phylogenetic tree is constructed from the core genome of the microbial species and a predefined set of genetic markers is selected from the auxiliary genome of the microbial species.

The invention relates to the crossing field of microorganism application and traditional art, in particular to a microorganism painting method combined with ceramic patterns. The problems that existing microbiological painting lines are rough, the artistic expressive force is weak, operation is difficult, and culture fusion is lacked are solved. The method comprises the following steps: screening and activating microbial strains capable of stably producing pigments; drawing a traditional ceramic pattern manuscript consistent with the culture dish in size; culturing the strain to a logarithmic phase, and calibrating the concentration of a bacterial suspension through a test pen to prepare'bio-ink '; dipping'bio-ink 'by using a sterilized trumpet wolf pen, and drawing on the culture medium by using a ceramic drawing pen method (outlining, spot dyeing and water diversion) according to the manuscript; sealed inverted culture can enhance the level through supplementary inoculation. According to the method, deep fusion of the traditional ceramic pattern and the microbial technology is achieved, the work is fine in line, rich in layer, easy and convenient to operate and low in pollution risk, scientific innovation and cultural connotation are achieved, and the method is suitable for teaching, science popularization and artistic creation.

The application relates to an integrated multifunctional integrated droplet microfluidic chip and application thereof, the integrated multifunctional integrated droplet microfluidic chip comprising a droplet generation area (1), a biochemical reaction incubation area (9) and a droplet sorting area (10). The application realizes the integrated function of single B cell droplet generation, incubation and sorting, meets the high-throughput demand, effectively reduces droplet fusion, is simple to operate and controllable in cost. The application also has important scientific application value in the fields of stem cell screening, immunotherapy CAR-T cell screening, microbial strain screening, organoid construction and other biological synthesis, disease diagnosis and microbial research.

The application discloses a kind of compound microbial inoculants for preharvest preservation of litchi and application thereof.The application is directed to the key enzymes of litchi pericarp browning and its pathogenic bacteria, and directional screening of microbial strains tolerating high concentration of metal ions, high yield of lignin-degrading enzyme and antifungal, etc. The compound microbial inoculants for preharvest preservation of litchi is formed and applied to litchi fruit growth period. After litchi is picked, the fruit size becomes larger, the shell skin is harder and not easy to be damaged, the inner wall white film is thicker, the natural oxidation speed is slower, the pulp is solid and crystal, the fruit core and pulp are connected tightly, in addition, the pericarp browning degree is delayed, the fruit disease rate and water loss rate are low. The application does not need any physical, chemical treatment or biological preservative after litchi is picked, which can prolong the natural preservation period after picking while keeping the original taste of the product. The application innovatively solves the problem of litchi preservation, and will produce important economic and social benefits after industrial application.

U I T T R E K S E L The present invention belongs to the field of agricultural microbial control technology, and specifically relates to a growth—promoting and disease—preventing compound microbial inoculant, a preparation method and an application thereof. The compound microbial inoculant specifically includes microbial powder, growth auxiliary materials, and. a desiccant. The microorganisms include Talaromyces flavus, Trichoderma harzianum, Bacillus velezensis, Bacillus licheniformis, and Brevibacillus laterosporus. Through the synergistic effect of multiple microbial strains, the compound microbial inoculant of the present invention enhances the control effect against Verticillium wilt pathogens, and overcomes the shortcomings of a single biocontrol microbe, such as a single biocontrol mechanism, poor environmental adaptability, inability to colonize in soil for a long time, and unstable control effect. Furthermore, the compound microbial inoculant of the present invention can increase the yields of cotton and pepper, and improve the fiber quality of cotton. The present invention provides scientific and technological support and decision-making services for realizing green, environmentally friendly, cost—saving, and efficiency-increasing modern agriculture.