330 results about "Microbiological strains" patented technology

The invention provides a petroleum hydrocarbon pollution remediation method based on soil microorganisms, and provides an innovative solution aiming at the problems of high cost, high energy consumption and secondary pollution of an existing remediation technology. The method comprises the following steps: firstly, carrying out sampling analysis and pretreatment on polluted soil, screening out a microbial strain capable of efficiently degrading petroleum hydrocarbon, and fixing the microbial strain on a biochar carrier to form an immobilized microbial composite material; then, the material is added into the contaminated soil, and tolerant plants are planted to construct a combined remediation system. Meanwhile, nutrient substances such as nitrogen and phosphorus and a surfactant are added, so that the bioavailability and the microbial degradation efficiency of the petroleum hydrocarbon are improved. In the repairing process, the petroleum hydrocarbon content and the microbial activity are monitored regularly, and the repairing scheme is dynamically adjusted. According to the method, the biochar is prepared from agricultural waste, the cost is reduced, efficient and environment-friendly remediation is achieved by combining the synergistic effect of microorganisms and plants, and the method has remarkable economic and environmental benefits and is suitable for treatment of soil polluted by various petroleum hydrocarbons.

The invention discloses a microbiological culture scheme customization recommendation method and system based on soil data analysis, and relates to the technical field of microbiological culture plants.The method comprises the steps that soil samples at different positions and depths are collected, soil characteristic data are analyzed, and data processing is carried out; microbial community information is obtained through soil DNA extraction, PCR amplification and high-throughput sequencing, and distribution and functions of microorganisms are evaluated in combination with data preprocessing and diversity analysis; selecting proper microbial strains according to soil characteristics and microbial community analysis results; a microbial culture scheme is combined with agricultural management measures; the conditions of soil and microorganisms are monitored in real time, and a microorganism culture scheme is dynamically adjusted based on a feedback mechanism; through field trials and advanced performance evaluation indexes, the effect of the microbial culture scheme is verified for a long time. The method has high adaptability and sustainability, and customized microorganism management schemes can be provided in different agricultural environments.

Platforms, systems, and methods for genetic generalization in synthetic biology development. According to one aspect, there is provided a method for predicting performance associated with genetic edits, the method comprising: receiving, by a platform, information about a strain of a microorganism, wherein the information about the strain comprises information describing a plurality of genetic edits to a base strain of the microorganism; generating, by the platform, a set of genetic embeddings based on the information about the strain, wherein the generating comprises processing the information about the strain using one or more embedding models, wherein each of the one or more embedding models: receives the information about the strain of the microorganism as input; and applies computational transformations to the input using a corresponding embedding model to generate a multi-dimensional vector representation for each of the plurality of genetic edits.

The invention discloses a ginseng fermentation product rich in various rare ginsenosides and a preparation method of the ginseng fermentation product. Aiming at the problem that the existing ginseng fermentation product has a certain effect in all aspects, but no effect is particularly clear and prominent, the ginseng fermentation product disclosed by the invention is obtained by fermenting a ginseng fermentation substrate by using a microbial strain, and comprises five ginsenosides, namely Rk1, Rg5, Rk3, Rh4 and Rg3, which have the effect of enhancing immunity; the total content of the five ginsenosides is more than 85% by weight, and the total content of the ginsenosides in the ginseng fermentation product is 100% by weight.

The invention discloses a tea fertilizer preparation method based on microbial fermentation, and particularly relates to the field of tea compost, tea waste is crushed and wetted, and a nitrogen source and trace elements are added to optimize the carbon nitrogen ratio, so that a foundation is laid for the fermentation process; then, selected microbial strains are proliferated and purified, and the treated waste materials are uniformly inoculated with the selected microbial strains; in the fermentation process, the stability of fermentation conditions is ensured by controlling the temperature and the humidity; the tannin concentration and the pH value are monitored in real time, when the tannin concentration exceeds a preset value and the pH deviates from a normal range, the abnormal state of tannin degradation is evaluated and identified through a reaction activity index, an interference source is positioned through a sparse principal component analysis algorithm, and noise signals are removed; and after the interference source is positioned, the fermentation condition is adjusted in a targeted manner, so that the tannin degradation efficiency reaches the expectation, and the compost is maturely fermented and meets the fertilizer use standard.

The present application discloses a recombinant bacterium for producing D-psicose by fermentation and a preparation method thereof, and belongs to the field of food chemical industry. The technical problem to be solved by the present application is: how to obtain a recombinant bacterium that uses methanol as a raw material for bacterial growth and glucose as a raw material to synthesize D-psicose. By subjecting the recipient bacteria to a purposeful transformation, the microbial strain is provided with the ability to accumulate microbial biomass using methanol and synthesize D-psicose using D-glucose, while preventing the use of D-glucose for microbial growth so that D-glucose is completely converted into D-psicose, thereby improving substrate utilization efficiency. The recombinant polymorphic Hansen yeast provided in the present application can realize the use of methanol and other compounds as raw materials for microbial growth, and after the microbial growth reaches a certain biomass, D-psicose is synthesized using D-glucose as a raw material. And then establish a corresponding technical route for synthesizing D-psicose.

The invention relates to the technical field of agricultural wastes and microorganisms, in particular to a complex microbial inoculant for high-temperature composting and rapid decomposition of livestock and poultry manure and straw cellulose and a preparation method of the complex microbial inoculant. The compound microorganism bacterial liquid comprises streptomyces thermocarbonic oxide, bacillus stearothermophilus, bacillus subtilis, bacillus licheniformis, phanerochaete chrysosporium, trichoderma viride and aspergillus niger, and the bacterial liquid carrier is obtained by compounding modified charcoal and modified sepiolite through amidation reaction. According to the complex microbial inoculant provided by the invention, through the synergistic effect of the seven functional complementary microbial strains and the special three-dimensional reticulate carrier, rapid decomposition of high-temperature compost of livestock and poultry manure and straw cellulose is realized.

The invention relates to bacillus velezensis LYB-S and application thereof in improving the reduction rate of hexavalent chromium in combination with exosomes, and belongs to the technical field of environmental restoration. The bacillus velezensis LYB-S has been preserved in the China General Microbiological Culture Collection Center (CGMCC) on May 6, 2025, and the preservation number is CGMCC NO.34429. The bacillus velezensis LYB-S has been preserved in the China General Microbiological Culture Collection Center (CGMCC). The bacillus velezensis LYB-S can be combined with an exosome, so that the reduction rate of the hexavalent chromium can be increased together with the bacillus velezensis LYB-S.

The invention discloses a preparation method of a micro-ecological bacterium enzyme preparation and application of the micro-ecological bacterium enzyme preparation in a feed additive, and belongs to the field of preparation of a fermented feed by combining a compound microorganism strain with a biological enzyme. Centrifugally separating and mixing bacterial liquid cultured by the acid-resistant zymophyte to obtain a mixed bacterial precipitate, and freeze-drying the mixed bacterial precipitate to obtain mixed bacterial powder; freezing a mixed enzyme solution formed by mixing papain, bromelain and cellulase to obtain mixed enzyme powder; and mixing the mixed bacterial powder and the mixed enzyme powder to form the micro-ecological bacterial enzyme preparation. Mixing and stirring the micro-ecological bacterium enzyme preparation, basal feed and water, and fermenting to obtain a fermentation mixture; adjusting the pH value of the fermentation mixture with hydrochloric acid, mixing with aldehyde chitosan, stirring and reacting to obtain a cross-linked fermentation product, and drying the cross-linked fermentation product with hot air to obtain the solid fermentation feed.

The invention discloses a special microbial fertilizer for cotton and a preparation method thereof, and belongs to the technical field of microbial fertilizers. The fertilizer is prepared from the following raw materials in parts by weight: 40-60 parts of a microbial agent, 80-100 parts of a microbial agent carrier, 40-60 parts of a nitrogen fertilizer, 30-50 parts of a phosphate fertilizer, 20-30 parts of a potassium fertilizer and 10-20 parts of a stabilizer, the microbial agent is prepared from nitrogen-fixing paenibacillus mucilaginosus MSSW01, bacillus nissi and streptomyces widermomole, and the microbial agent is prepared from bacillus mucilaginosus MSSW01 and streptomyces widermomole. The stabilizer is prepared from roxburgh rose extract, soybean protein isolate and gamma-aminobutyric acid according to the mass ratio of 2: 1: (0.2-0.5). By screening and optimizing three beneficial microbial strains and combining the synergistic effect of raw material components in a specific ratio, the yield and quality improvement of cotton can be remarkably promoted, and meanwhile, the occurrence rate of common diseases of cotton is effectively reduced.

The invention relates to the technical field of fertilizers, and particularly discloses a bacterial strain capable of reducing the function of vomitoxin of wheat and corn and application of the bacterial strain, the bacterial strain capable of reducing the function of vomitoxin of wheat and corn is applied to preparation of a fertilizer, 10%-90% of bacillus velezensis XNYF-1 fermentation liquor (A liquor) and 10%-90% of pseudomonas fluorescens XNYF-2 fermentation liquor (B liquor) are mixed to prepare AB mother liquor, and the AB mother liquor is used for preparing the fertilizer. The XNYF-1 and the XNYF-2 are two functional microbial strains which are subjected to screening mutagenesis and can be used for producing chitinase, iturin, various phenazine derivatives and biological surfactin respectively, and the XNYF-1 and the XNYF-2 can be used for producing the chitinase, the iturin, the various phenazine derivatives and the biological surfactin respectively. And the metabolite has a good biocontrol effect.

The invention provides a salt-tolerant bacillus and application thereof, the strain is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.29259. A fermentation product obtained by using the strain has remarkable effects of moisturizing, whitening, relieving, resisting oxidation, resisting wrinkles, controlling oil, removing acnes and tightening, and can be applied to cosmetics.

The invention belongs to the technical field of microorganisms, and particularly discloses a morchella growth-promoting strain Clonostachys roseMSF05, the classification name is Clonostachys rosea, and the preservation number is GDMCC No: 65937, morchella is used as a precious edible and medicinal fungus, the planting scale is continuously enlarged, but the soil continuous cropping problem has become a main obstacle for soil-covered cultivation and fruiting of the morchella, the soil microorganism structure of the morchella is studied, and the morchella growth-promoting strain CClonostachys roseMSF05 can be used for promoting growth of the morchella. And the beneficial microbial strain clonostachys rosea MSF05 is screened out, so that technical support is provided for rebuilding soil micro-ecology, relieving continuous cropping obstacles of morchella esculenta and biological prevention and control.

The invention discloses a microbial remediation method and a microbial remediation system. The microbial remediation method comprises the following steps: collecting samples from a contaminated site, carrying out enrichment culture, screening and domestication to obtain various microbial strains, preparing a composite microbial agent according to a specific ratio, carrying out carrier immobilization treatment to form immobilized microbial particles, applying the immobilized microbial particles to soil in the modes of strip application, hole application and the like according to the contamination condition, or filling a suspended filler and putting the suspended filler into a water body. And environmental parameters are monitored in real time, and accurate regulation and control are performed to promote microorganisms to degrade pollutants. The microbial remediation system covers microbial culture and microbial agent preparation, putting and distribution, monitoring and regulation modules and the like, and automation and intelligentization of the whole process are achieved. The method is high in remediation efficiency, high in adaptability, low in cost, environmentally friendly and capable of effectively solving the problem of soil and water pollution.

The invention discloses a ginseng fermentation product rich in various ginsenosides as well as a preparation method and application of the ginseng fermentation product. The ginseng fermentation product provided by the invention is obtained by fermenting a ginseng fermentation substrate by using a microbial strain. The ginseng fermentation product aims at the problem that existing ginseng fermentation products have certain effects in the aspects of whitening, resisting inflammation, enhancing the immunity of the organism and the like, but do not have specific and prominent effects. The content of anti-inflammatory rare ginsenosides Rk1, Rg5, Rk3 and Rh4 in the ginseng fermentation product reaches up to 50 wt% or above, and the relative content of ginsenosides Rk3 and Rh4 which are not prone to causing sleepiness reaches up to 30 wt% or above.

The invention discloses a process for repairing damaged soil ecology by lincomycin waste liquid fertilizer, and relates to the technical field of soil remediation, which comprises the following specific steps: step 1, screening and culturing microbial decomposition flora, the invention has the following advantages: through the chelation of the polyaspartic acid derivative on metal ions and the promotion effect on nutrient absorption, the release and transmission of nutrients are precisely regulated and controlled, so that crops can absorb and utilize the nutrients more efficiently; the nano composite material and the specific biological stimulant containing various beneficial microbial strains and natural plant extracts are jointly fused into the liquid fertilizer, the microbial strains in the specific biological stimulant activate the soil biological environment, the natural plant extracts assist plant growth to resist diseases, and the nano composite material and the natural plant extracts have a synergistic effect, so that the liquid fertilizer has a good application prospect. Physicochemical properties and biological activity of soil are optimized, excellent soil micro-ecology is created for plant growth, healthy and strong growth of plants is promoted, and restoration of damaged soil is enhanced.

The invention relates to a method for extracting rare earth elements based on a biological separation method, and relates to the technical field of rare earth element separation and extraction. In order to solve the problems of high pollution and poor selectivity of a traditional chemical method and low adsorption capacity, insufficient stability and the like of a biotechnology, the rare earth ion adsorbent is prepared by screening microbial strains or biomolecules with high affinity, utilizing the specific adsorption or metabolic acid production dissolution effect of surface functional groups of the microbial strains or biomolecules on rare earth ions and combining biological material modification and a combined process. And efficient enrichment and selective separation of target elements in low-grade rare earth ore, multi-metal complex ore and rare earth-containing wastewater are realized. According to the technology, key parameter regulation and control are optimized through a process kinetic model, energy consumption and environmental pollution are reduced, meanwhile, the rare earth recovery purity and process stability are improved, and an innovative solution is provided for green and sustainable development of rare earth resources.

The utility model relates to the field of microbial fermentation, and discloses a microbial strain fermentation device convenient for material supplementation, which comprises a fermentation tank, the fermentation tank is provided with a fermentation inner cavity, and the top end and the bottom end of the fermentation tank are respectively and fixedly connected with a feed pipe and a discharge pipe which are communicated with the fermentation inner cavity. A plurality of material supplementing pipes communicated with the fermentation inner cavity are fixedly mounted at the top end of the fermentation tank along the outer side of the feeding pipe, two semicircular connecting arc blocks are fixedly mounted at the top ends of the material supplementing pipes, and the two ends of the two connecting arc blocks are fixedly connected with a plurality of bolts through a plurality of mounting blocks respectively; the top end of the fermentation tank is sleeved with a rubber sleeve located on the inner sides of the two connecting arc blocks, the top ends of the two closed connecting arc blocks are rotationally connected with a protection assembly, and the possibility of being polluted by external microorganisms can be reduced to a great extent while material supplementation is facilitated.

The present invention provides a long-period high-pressure enrichment culture and online monitoring apparatus and method for deep-sea microorganisms. The apparatus can simulate extreme deep-sea environments such as high pressure, low temperature, and oligotrophic conditions. Through pre-culture and graded serial culture, stable long-period growth and reproduction of the deep-sea microorganisms are ensured. An online system is used for monitoring the abundance, activity, and culture conditions of the microorganisms in real time to determine an appropriate timing for strain subculturing, and timely interactive subculturing and culture are performed, thereby achieving stable supply of high-abundance, high-activity microorganism enrichment culture solution. The present invention achieves precise pressure-retaining abundance counting of the microorganisms under in-situ pressure conditions and accurate control of a microbial growth cycle during enrichment culture, effectively improving timeliness and culture efficiency of the microorganism enrichment culture solution, and enabling long-period acquisition of high-abundance, high-activity microbial strain liquid.

The invention relates to the field of microbial fermented food, and discloses lactobacillus plantarum and application thereof, and fermented walnut yoghourt and a preparation method thereof. The preservation number of the lactobacillus plantarum provided by the invention is CCTCC (China Center For Type Culture Collection) NO: M 20242279. The lactobacillus plantarum with better performance is obtained from the nature, the lactobacillus plantarum is compounded with other yoghourt fermentation probiotics for fermentation to prepare the walnut yoghourt, and compared with a contrast fermented product of a single strain and a compound microorganism strain, the alpha-glucosidase inhibition rate is remarkably improved, and the walnut yoghourt has the advantages that the alpha-glucosidase inhibition rate is remarkably increased; excellent activity of improving blood sugar is shown. The invention not only provides an effective, time-saving and energy-saving preparation process method of the walnut yoghurt, but also provides a wide foundation for further application of walnuts.

The invention belongs to the technical field of agriculture and relates to microorganisms, in particular to a compound microbial agent and application thereof. The invention relates to a compound microbial agent, which is prepared from slow-growing rhizobium HS3, pseudomonas Y43, burkholderia Y30 and agrobacterium Y27. The compound microbial agent NBI prepared from efficient rhizobium and beneficial microbial strains adapting to local soil is preferably selected by combining soil types and microbial adaptability of different regions in Hainan province, and can be used for improving nodulation and nitrogen fixation capacity of fresh soybeans, promoting the yield of the soybeans, reducing application of chemical fertilizers and improving the per unit yield of the soybeans.

The invention relates to a multifunctional pseudomonas Y2-2 and application thereof, the strain is a multifunctional microbial strain, can effectively utilize microorganisms to solve the problem of heavy metal environmental pollution remediation, can also restrain various plant diseases and insect pests, and is classified and named as Pseudomonas sp. The strain is preserved in China General Microbiological Culture Collection Center (CGMCC) on April 21, 2025, the address is Institute of Microbiology, Chinese Academy of Sciences, No.3, No.1 Yard, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC No.34287. The method can effectively utilize microorganisms to repair heavy metal environmental pollution, can inhibit various plant diseases and insect pests, is good in effect and high in efficiency, and has practical popularization and application values.

The invention discloses a stress-resistant growth-promoting stable fertilizer and a preparation method thereof, and belongs to the technical field of bio-organic fertilizers. The bio-organic fertilizer is prepared by taking soybean meal leavening, pomegranate peel residues, eucommia ulmoides leaf residues, a compound microorganism bacterium solution and a microorganism activating agent as raw materials, two high-salt-tolerance growth-promoting microorganism strains are screened to form a functional microorganism, madura anactinomyces activates the enzyme production activity of salt-tolerant bacillus by secreting metabolite, and the biological organic fertilizer is prepared. And organic matters decomposed by the salt-tolerant bacillus reversely provide a growth substrate for the madura anactinomycetes to form a metabolism reciprocal relationship. The fertilizer provided by the invention can effectively regulate and stabilize soil activity and promote crop growth, can effectively improve plant stress resistance related enzyme activity, root activity and carotenoid content in leaves under salt stress, improves resistance of soybean plants to salt stress adversity conditions, and has wide development potential and application value.

The present invention relates to the field of microbial technology, and discloses Rhizopus microsporus HR3-26 and its application in the extraction of boletus polysaccharide. The present invention provides a new microbial strain, Rhizopus microsporus HR3-26, and allows Rhizopus microsporus HR3-26 to grow moderately in boletus powder added with sucrose to produce various hydrolases. After that, the fermented boletus powder is heated with water, and substances such as cellulose in the cell wall are hydrolyzed, which helps the bound boletus polysaccharide to dissolve during ultrasonic water extraction, thereby significantly improving the extraction yield of polysaccharide. The present invention applies Rhizopus microsporus HR3-26 to the extraction of boletus polysaccharide. Compared with the conventional method of water extraction and alcohol precipitation, the extraction yield of polysaccharide can be increased by 41.3%.

The invention provides a methylobacterium strain JC03 for producing single-cell protein and application of the methylobacterium strain JC03 in the field of aquaculture, and belongs to the technical field of microorganisms. The methylobacterium strain JC03 is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.32616. The strain is high in growth efficiency, high in thallus crude protein content, rich in amino acid spectrum, high in essential amino acid content and rich in functional components for improving the immunocompetence of aquatic animals; the single-cell protein produced by the strain can completely or partially replace fish meal protein in feeds of marine fishes such as turbot, pseudosciaena crocea, grouper and rainbow trout; vegetable protein in feed of freshwater fish such as grass carp can be completely replaced; animal and plant proteins in feeds for shrimps and crabs, such as penaeus vannamei, can be completely replaced, and animal and plant proteins in stichopus japonicus feeds can be completely replaced; the immunocompetence of fed aquatic animals can be obviously improved. The invention provides a microbial strain for efficiently converting methanol to produce single-cell protein, and the microbial strain has a wide application prospect in the field of aquaculture.

The invention relates to a large-scale high-yield and high-efficiency cultivation mode of pumpkins in saline-alkali soil, which adopts a composite modifier to improve the saline-alkali soil, and the composite modifier contains an organic fertilizer, a soil conditioner and a saline-alkali tolerant microbial strain in a specific proportion, so that the soil structure and fertility are effectively improved; a pumpkin variety with a salt-tolerant gene marker is selected, and the salt tolerance and the survival rate of seedlings are remarkably improved by combining a biochar seedling raising technology; through an intelligent drip irrigation system and a precise fertilization technology, the irrigation and fertilization amount is dynamically adjusted according to the growth stage of pumpkins and soil fertility, and the resource utilization efficiency is improved; a disease and pest control method combining physical and biological control is adopted, meanwhile, an S-shaped vine pressing method is combined to promote plant growth, and product quality tracing is achieved through a block chain technology. The problem of pumpkin planting in the saline-alkali soil is effectively solved, the yield and quality of pumpkins are remarkably improved, remarkable economic benefits and ecological benefits are achieved, and a new thought and method are provided for agricultural development of the saline-alkali soil.

The invention provides compound bacteria for biological enhancement and sludge reduction and a preparation method of the compound bacteria. The compound microbial preparation comprises 25 microbial strains capable of widely degrading organic compounds and sludge zoogloea, original strains with specific degradation capability are subjected to multi-step pure culture fermentation under limited conditions and then are compounded to obtain the compound microbial agent. The composite microbial preparation can be used for degrading various organic matters including aromatic hydrocarbons, alcohols, aldehydes, heterocyclic rings, halogenated hydrocarbons, anilines, phenols and the like and aged activated sludge zoogloea, and can be used for synergism and sludge in-situ reduction of biological systems of various industrial wastewater including municipal wastewater, petrochemical wastewater, synthetic chemistry, printing and dyeing wastewater and the like. Due to the excellent extracellular hydrolase producing function of the compound bacteria, the sludge yield of an existing biochemical system can be remarkably reduced, and in-situ reduction of the sludge is achieved.

The invention provides a konjac continuous cropping modifier based on rhizosphere microflora regulation and control, and relates to the technical field of plant planting reagents, the modifier comprises the following components: actinomycetes, nitrogen-fixing bacteria, phosphate solubilizing bacteria, potassium solubilizing bacteria, disease-resistant fungi, humic acid and seaweed extract, an organic carrier and a mineral supplement, microbial strains with different functions such as actinomycetes, nitrogen-fixing bacteria, phosphate solubilizing bacteria, potassium solubilizing bacteria and disease-resistant fungi can be colonized in soil, and nitrogen, phosphorus and potassium elements in the soil are decomposed by inhibiting harmful pathogenic bacteria, so that the nutrient structure of the soil is improved, and the biological activity of the soil is enhanced. The synergistic effect of microorganisms effectively enhances the disease resistance of the soil and solves the problem of continuous cropping soil deterioration.

The invention belongs to the technical field of microbial strains and environmental microbiology, and discloses a novel strain capable of degrading tetracycline antibiotics. The new bacterial strain is an Enterobacter hormaechei SD7-1 bacterial strain, and is preserved in the Guangdong Microbial Culture Collection Center on March 3, 2025, and the preservation number is GDMCC No: 65849. The invention further discloses a preparation method of the new bacterial strain. The new strain provided by the invention has a remarkable degradation effect on tetracycline antibiotics, and the degradation rate of tetracycline after 24-hour treatment reaches 50% or above. Therefore, as a functional strain, the enterobacter hormaechei SD7-1 strain has high degradation efficiency and a wide degradation spectrum, can effectively degrade various tetracycline antibiotics in a short time, and has the advantages of treating pollution caused by wide use of tetracycline, further reducing environmental half-life emission of tetracycline, protecting the environment, and having good application prospects. The invention provides a novel microbial resource with potential in soil and water environments, especially in soil and water environments.