275 results about "Microbiological strains" patented technology

The invention discloses a microbiological culture scheme customization recommendation method and system based on soil data analysis, and relates to the technical field of microbiological culture plants.The method comprises the steps that soil samples at different positions and depths are collected, soil characteristic data are analyzed, and data processing is carried out; microbial community information is obtained through soil DNA extraction, PCR amplification and high-throughput sequencing, and distribution and functions of microorganisms are evaluated in combination with data preprocessing and diversity analysis; selecting proper microbial strains according to soil characteristics and microbial community analysis results; a microbial culture scheme is combined with agricultural management measures; the conditions of soil and microorganisms are monitored in real time, and a microorganism culture scheme is dynamically adjusted based on a feedback mechanism; through field trials and advanced performance evaluation indexes, the effect of the microbial culture scheme is verified for a long time. The method has high adaptability and sustainability, and customized microorganism management schemes can be provided in different agricultural environments.

Platforms, systems, and methods for genetic generalization in synthetic biology development. According to one aspect, there is provided a method for predicting performance associated with genetic edits, the method comprising: receiving, by a platform, information about a strain of a microorganism, wherein the information about the strain comprises information describing a plurality of genetic edits to a base strain of the microorganism; generating, by the platform, a set of genetic embeddings based on the information about the strain, wherein the generating comprises processing the information about the strain using one or more embedding models, wherein each of the one or more embedding models: receives the information about the strain of the microorganism as input; and applies computational transformations to the input using a corresponding embedding model to generate a multi-dimensional vector representation for each of the plurality of genetic edits.

The invention relates to the technical field of agricultural wastes and microorganisms, in particular to a complex microbial inoculant for high-temperature composting and rapid decomposition of livestock and poultry manure and straw cellulose and a preparation method of the complex microbial inoculant. The compound microorganism bacterial liquid comprises streptomyces thermocarbonic oxide, bacillus stearothermophilus, bacillus subtilis, bacillus licheniformis, phanerochaete chrysosporium, trichoderma viride and aspergillus niger, and the bacterial liquid carrier is obtained by compounding modified charcoal and modified sepiolite through amidation reaction. According to the complex microbial inoculant provided by the invention, through the synergistic effect of the seven functional complementary microbial strains and the special three-dimensional reticulate carrier, rapid decomposition of high-temperature compost of livestock and poultry manure and straw cellulose is realized.

The invention relates to bacillus velezensis LYB-S and application thereof in improving the reduction rate of hexavalent chromium in combination with exosomes, and belongs to the technical field of environmental restoration. The bacillus velezensis LYB-S has been preserved in the China General Microbiological Culture Collection Center (CGMCC) on May 6, 2025, and the preservation number is CGMCC NO.34429. The bacillus velezensis LYB-S has been preserved in the China General Microbiological Culture Collection Center (CGMCC). The bacillus velezensis LYB-S can be combined with an exosome, so that the reduction rate of the hexavalent chromium can be increased together with the bacillus velezensis LYB-S.

The invention discloses a special microbial fertilizer for cotton and a preparation method thereof, and belongs to the technical field of microbial fertilizers. The fertilizer is prepared from the following raw materials in parts by weight: 40-60 parts of a microbial agent, 80-100 parts of a microbial agent carrier, 40-60 parts of a nitrogen fertilizer, 30-50 parts of a phosphate fertilizer, 20-30 parts of a potassium fertilizer and 10-20 parts of a stabilizer, the microbial agent is prepared from nitrogen-fixing paenibacillus mucilaginosus MSSW01, bacillus nissi and streptomyces widermomole, and the microbial agent is prepared from bacillus mucilaginosus MSSW01 and streptomyces widermomole. The stabilizer is prepared from roxburgh rose extract, soybean protein isolate and gamma-aminobutyric acid according to the mass ratio of 2: 1: (0.2-0.5). By screening and optimizing three beneficial microbial strains and combining the synergistic effect of raw material components in a specific ratio, the yield and quality improvement of cotton can be remarkably promoted, and meanwhile, the occurrence rate of common diseases of cotton is effectively reduced.

The invention relates to the technical field of fertilizers, and particularly discloses a bacterial strain capable of reducing the function of vomitoxin of wheat and corn and application of the bacterial strain, the bacterial strain capable of reducing the function of vomitoxin of wheat and corn is applied to preparation of a fertilizer, 10%-90% of bacillus velezensis XNYF-1 fermentation liquor (A liquor) and 10%-90% of pseudomonas fluorescens XNYF-2 fermentation liquor (B liquor) are mixed to prepare AB mother liquor, and the AB mother liquor is used for preparing the fertilizer. The XNYF-1 and the XNYF-2 are two functional microbial strains which are subjected to screening mutagenesis and can be used for producing chitinase, iturin, various phenazine derivatives and biological surfactin respectively, and the XNYF-1 and the XNYF-2 can be used for producing the chitinase, the iturin, the various phenazine derivatives and the biological surfactin respectively. And the metabolite has a good biocontrol effect.

The invention belongs to the technical field of microorganisms, and particularly discloses a morchella growth-promoting strain Clonostachys roseMSF05, the classification name is Clonostachys rosea, and the preservation number is GDMCC No: 65937, morchella is used as a precious edible and medicinal fungus, the planting scale is continuously enlarged, but the soil continuous cropping problem has become a main obstacle for soil-covered cultivation and fruiting of the morchella, the soil microorganism structure of the morchella is studied, and the morchella growth-promoting strain CClonostachys roseMSF05 can be used for promoting growth of the morchella. And the beneficial microbial strain clonostachys rosea MSF05 is screened out, so that technical support is provided for rebuilding soil micro-ecology, relieving continuous cropping obstacles of morchella esculenta and biological prevention and control.

The invention discloses a microbial remediation method and a microbial remediation system. The microbial remediation method comprises the following steps: collecting samples from a contaminated site, carrying out enrichment culture, screening and domestication to obtain various microbial strains, preparing a composite microbial agent according to a specific ratio, carrying out carrier immobilization treatment to form immobilized microbial particles, applying the immobilized microbial particles to soil in the modes of strip application, hole application and the like according to the contamination condition, or filling a suspended filler and putting the suspended filler into a water body. And environmental parameters are monitored in real time, and accurate regulation and control are performed to promote microorganisms to degrade pollutants. The microbial remediation system covers microbial culture and microbial agent preparation, putting and distribution, monitoring and regulation modules and the like, and automation and intelligentization of the whole process are achieved. The method is high in remediation efficiency, high in adaptability, low in cost, environmentally friendly and capable of effectively solving the problem of soil and water pollution.

The invention relates to a method for extracting rare earth elements based on a biological separation method, and relates to the technical field of rare earth element separation and extraction. In order to solve the problems of high pollution and poor selectivity of a traditional chemical method and low adsorption capacity, insufficient stability and the like of a biotechnology, the rare earth ion adsorbent is prepared by screening microbial strains or biomolecules with high affinity, utilizing the specific adsorption or metabolic acid production dissolution effect of surface functional groups of the microbial strains or biomolecules on rare earth ions and combining biological material modification and a combined process. And efficient enrichment and selective separation of target elements in low-grade rare earth ore, multi-metal complex ore and rare earth-containing wastewater are realized. According to the technology, key parameter regulation and control are optimized through a process kinetic model, energy consumption and environmental pollution are reduced, meanwhile, the rare earth recovery purity and process stability are improved, and an innovative solution is provided for green and sustainable development of rare earth resources.

The present invention provides a long-period high-pressure enrichment culture and online monitoring apparatus and method for deep-sea microorganisms. The apparatus can simulate extreme deep-sea environments such as high pressure, low temperature, and oligotrophic conditions. Through pre-culture and graded serial culture, stable long-period growth and reproduction of the deep-sea microorganisms are ensured. An online system is used for monitoring the abundance, activity, and culture conditions of the microorganisms in real time to determine an appropriate timing for strain subculturing, and timely interactive subculturing and culture are performed, thereby achieving stable supply of high-abundance, high-activity microorganism enrichment culture solution. The present invention achieves precise pressure-retaining abundance counting of the microorganisms under in-situ pressure conditions and accurate control of a microbial growth cycle during enrichment culture, effectively improving timeliness and culture efficiency of the microorganism enrichment culture solution, and enabling long-period acquisition of high-abundance, high-activity microbial strain liquid.

The invention relates to the field of microbial fermented food, and discloses lactobacillus plantarum and application thereof, and fermented walnut yoghourt and a preparation method thereof. The preservation number of the lactobacillus plantarum provided by the invention is CCTCC (China Center For Type Culture Collection) NO: M 20242279. The lactobacillus plantarum with better performance is obtained from the nature, the lactobacillus plantarum is compounded with other yoghourt fermentation probiotics for fermentation to prepare the walnut yoghourt, and compared with a contrast fermented product of a single strain and a compound microorganism strain, the alpha-glucosidase inhibition rate is remarkably improved, and the walnut yoghourt has the advantages that the alpha-glucosidase inhibition rate is remarkably increased; excellent activity of improving blood sugar is shown. The invention not only provides an effective, time-saving and energy-saving preparation process method of the walnut yoghurt, but also provides a wide foundation for further application of walnuts.

The invention belongs to the technical field of agriculture and relates to microorganisms, in particular to a compound microbial agent and application thereof. The invention relates to a compound microbial agent, which is prepared from slow-growing rhizobium HS3, pseudomonas Y43, burkholderia Y30 and agrobacterium Y27. The compound microbial agent NBI prepared from efficient rhizobium and beneficial microbial strains adapting to local soil is preferably selected by combining soil types and microbial adaptability of different regions in Hainan province, and can be used for improving nodulation and nitrogen fixation capacity of fresh soybeans, promoting the yield of the soybeans, reducing application of chemical fertilizers and improving the per unit yield of the soybeans.

The invention relates to a multifunctional pseudomonas Y2-2 and application thereof, the strain is a multifunctional microbial strain, can effectively utilize microorganisms to solve the problem of heavy metal environmental pollution remediation, can also restrain various plant diseases and insect pests, and is classified and named as Pseudomonas sp. The strain is preserved in China General Microbiological Culture Collection Center (CGMCC) on April 21, 2025, the address is Institute of Microbiology, Chinese Academy of Sciences, No.3, No.1 Yard, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC No.34287. The method can effectively utilize microorganisms to repair heavy metal environmental pollution, can inhibit various plant diseases and insect pests, is good in effect and high in efficiency, and has practical popularization and application values.

The invention discloses a stress-resistant growth-promoting stable fertilizer and a preparation method thereof, and belongs to the technical field of bio-organic fertilizers. The bio-organic fertilizer is prepared by taking soybean meal leavening, pomegranate peel residues, eucommia ulmoides leaf residues, a compound microorganism bacterium solution and a microorganism activating agent as raw materials, two high-salt-tolerance growth-promoting microorganism strains are screened to form a functional microorganism, madura anactinomyces activates the enzyme production activity of salt-tolerant bacillus by secreting metabolite, and the biological organic fertilizer is prepared. And organic matters decomposed by the salt-tolerant bacillus reversely provide a growth substrate for the madura anactinomycetes to form a metabolism reciprocal relationship. The fertilizer provided by the invention can effectively regulate and stabilize soil activity and promote crop growth, can effectively improve plant stress resistance related enzyme activity, root activity and carotenoid content in leaves under salt stress, improves resistance of soybean plants to salt stress adversity conditions, and has wide development potential and application value.

The present invention relates to the field of microbial technology, and discloses Rhizopus microsporus HR3-26 and its application in the extraction of boletus polysaccharide. The present invention provides a new microbial strain, Rhizopus microsporus HR3-26, and allows Rhizopus microsporus HR3-26 to grow moderately in boletus powder added with sucrose to produce various hydrolases. After that, the fermented boletus powder is heated with water, and substances such as cellulose in the cell wall are hydrolyzed, which helps the bound boletus polysaccharide to dissolve during ultrasonic water extraction, thereby significantly improving the extraction yield of polysaccharide. The present invention applies Rhizopus microsporus HR3-26 to the extraction of boletus polysaccharide. Compared with the conventional method of water extraction and alcohol precipitation, the extraction yield of polysaccharide can be increased by 41.3%.

The invention relates to a large-scale high-yield and high-efficiency cultivation mode of pumpkins in saline-alkali soil, which adopts a composite modifier to improve the saline-alkali soil, and the composite modifier contains an organic fertilizer, a soil conditioner and a saline-alkali tolerant microbial strain in a specific proportion, so that the soil structure and fertility are effectively improved; a pumpkin variety with a salt-tolerant gene marker is selected, and the salt tolerance and the survival rate of seedlings are remarkably improved by combining a biochar seedling raising technology; through an intelligent drip irrigation system and a precise fertilization technology, the irrigation and fertilization amount is dynamically adjusted according to the growth stage of pumpkins and soil fertility, and the resource utilization efficiency is improved; a disease and pest control method combining physical and biological control is adopted, meanwhile, an S-shaped vine pressing method is combined to promote plant growth, and product quality tracing is achieved through a block chain technology. The problem of pumpkin planting in the saline-alkali soil is effectively solved, the yield and quality of pumpkins are remarkably improved, remarkable economic benefits and ecological benefits are achieved, and a new thought and method are provided for agricultural development of the saline-alkali soil.

The invention provides compound bacteria for biological enhancement and sludge reduction and a preparation method of the compound bacteria. The compound microbial preparation comprises 25 microbial strains capable of widely degrading organic compounds and sludge zoogloea, original strains with specific degradation capability are subjected to multi-step pure culture fermentation under limited conditions and then are compounded to obtain the compound microbial agent. The composite microbial preparation can be used for degrading various organic matters including aromatic hydrocarbons, alcohols, aldehydes, heterocyclic rings, halogenated hydrocarbons, anilines, phenols and the like and aged activated sludge zoogloea, and can be used for synergism and sludge in-situ reduction of biological systems of various industrial wastewater including municipal wastewater, petrochemical wastewater, synthetic chemistry, printing and dyeing wastewater and the like. Due to the excellent extracellular hydrolase producing function of the compound bacteria, the sludge yield of an existing biochemical system can be remarkably reduced, and in-situ reduction of the sludge is achieved.

The invention belongs to the technical field of microbial strains and environmental microbiology, and discloses a novel strain capable of degrading tetracycline antibiotics. The new bacterial strain is an Enterobacter hormaechei SD7-1 bacterial strain, and is preserved in the Guangdong Microbial Culture Collection Center on March 3, 2025, and the preservation number is GDMCC No: 65849. The invention further discloses a preparation method of the new bacterial strain. The new strain provided by the invention has a remarkable degradation effect on tetracycline antibiotics, and the degradation rate of tetracycline after 24-hour treatment reaches 50% or above. Therefore, as a functional strain, the enterobacter hormaechei SD7-1 strain has high degradation efficiency and a wide degradation spectrum, can effectively degrade various tetracycline antibiotics in a short time, and has the advantages of treating pollution caused by wide use of tetracycline, further reducing environmental half-life emission of tetracycline, protecting the environment, and having good application prospects. The invention provides a novel microbial resource with potential in soil and water environments, especially in soil and water environments.

The invention relates to the field of microbial strains edited by gene recombination and synthetic biology, and provides recombinant escherichia coli for synthesizing D-xylose by using glucose and application. The recombinant escherichia coli comprises the following modifications: exogenous insertion of a gene IRX for coding beta-1, 4-xylosyltransferase; exogenous insertion of a gene UXS encoding a UDP-glucuronic acid decarboxylase; the invention relates to enhanced expression or overexpression of a gene yagH for coding beta xylosidase. Compared with a traditional hemicellulose hydrolysis method for producing D-xylose, the method for synthesizing and preparing D-xylose through whole-cell catalysis of D-glucose by using the recombinant escherichia coli has the advantages of environmental protection, cost advantage and energy saving.

The invention relates to the technical field of compound microbial additives, and particularly discloses a compound microbial additive suitable for alpine regions and a preparation method, the additive comprises microbial thalli, a protective agent and a carrier; the microorganisms are low-temperature-resistant nitrogen-fixing bacteria, cold-resistant phosphate-solubilizing bacteria, cold-resistant potassium-solubilizing bacteria, low-temperature-resistant plant growth-promoting rhizobacteria and cold-resistant streptomyces; the selected microbial strains are all derived from extreme environments of the alpine regions, are naturally selected and artificially domesticated for a long time, and have extremely strong adaptability to severe conditions such as low temperature and oxygen deficit of the alpine regions. Compared with the existing microbial additive in the market, the growth rate and metabolic activity of the microbial strain in the additive are kept at a relatively high level in a low-temperature environment of 0-15 DEG C.

According to the AI-assisted marine microorganism anti-tumor activity product separation method provided by the invention, a microorganism strain with high anti-tumor activity potential can be directly and quickly screened out by constructing a marine microorganism sample database and applying a trained HetGNN model; the workload and time cost of tedious fermentation culture and biological activity testing in a traditional screening process are greatly reduced, meanwhile, the screening period is shortened, and the screening efficiency is greatly improved. Besides, a closed-loop feedback system formed by the invention feeds back a high-purity target compound sample separated from a laboratory and related data thereof to a microorganism sample database, and continuously optimizes the integrity of the database and the performance of the HetGNN model, so that the accuracy and efficiency of subsequent anti-tumor active compound screening are remarkably improved; the problem that in the prior art, screening precision is limited by initial data, and iterative upgrading is difficult is solved.

The invention belongs to the technical field of environmental restoration, and particularly relates to a modified fulvic acid-charcoal immobilized microbial agent as well as a preparation method and application thereof. The preparation method comprises the following steps: adding a bacterial liquid, modified fulvic acid and biochar into a culture medium for co-culture to obtain the modified fulvic acid-biochar immobilized bacterial agent. The preparation method is simple, convenient, easy to control and environmentally friendly, the cost is reduced, meanwhile, the prepared modified fulvic acid-biochar immobilized microbial agent still shows high microbial strain activity, petroleum hydrocarbon and polycyclic aromatic hydrocarbon in combined pollution are efficiently degraded and can be maintained for a long time, and good industrial application prospects are achieved.

According to the invention, four specific strains are obtained by separating, purifying and culturing from rhizosphere soil of angelica sinensis seedlings, and are identified as bacillus and pseudomonas by virtue of microbe 16S rDNA (ribosomal deoxyribonucleic acid) sequencing, and the four specific strains are respectively bacillus subtilis NF6, Pseudomonas koreensis ISP16, Pseudomonas germanica IAA17 and Pseudomonas alli KPS68. The strain is preserved in China Center for Type Culture Collection in Wuhan University Life Science College, Wuhan, Hubei, China; the strain is preserved in January 15, 2025, the postcode of the strain is 430072, the preservation date of the strain is January 15, 2025, and the preservation numbers of the strain are respectively CCTCC M 2025155, CCTCC M 2025156, CCTCC M 2025157 and CCTCC M 2025158. The strain obtained by the invention can be used for preparing a green, environment-friendly, efficient, low-toxicity and high-cost-performance microbial agent for promoting growth of angelica sinensis and preventing and treating early bolting of angelica sinensis, has important environmental protection significance and economic value for increasing the yield of angelica sinensis, and is worthy of popularization and application.

The invention discloses bacillus megaterium and application thereof in prevention and treatment of cucumber downy mildew. The bacillus megaterium ZF521 for preventing and treating the cucumber downy mildew, provided by the invention, has the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.31036 in the General Microbiological Culture Collection Center of the China Committee for Culture Collection of Microorganisms. A potted living body test shows that the highest prevention and treatment effect of the bacillus megaterium on the cucumber downy mildew can reach 72.62%, meanwhile, the bacillus megaterium CGMCC No.31036 can promote the increase of the content of three endogenous hormones in the cucumber, and the bacillus megaterium CGMCC No.31036 is applied to the cucumber, so that the cucumber downy mildew can be effectively prevented and treated, and the growth of the cucumber is promoted.

This invention relates to a microbial agent with highly efficient plastic degradation properties and its application, wherein the microbial agent contains *Cryptococcus lauriensis* strain with accession number CCTCC NO: M 2026232. Papiliotrema laurentii SNP7-2P strain and / or Achromobacterium with accession number CCTCC NO: M 2026231 Achromobacter strains FJ1-2L. These two microbial strains exhibit varying degrees of degradation effects on long-chain polymers in plastics, effectively and environmentally addressing plastic pollution and making them suitable for preparing green and environmentally friendly degradable plastic products.

The invention relates to the technical field of biological fermentation, in particular to a method for improving the fermentation stability of vitamin B12, which comprises the following steps: acquiring a microbial strain of vitamin B12 and activating; preparing a culture medium suitable for fermentation of vitamin B12 according to a composite carbon source system, an optimized nitrogen source combination, key elements, other nutritional ingredients, plant three elements and a modified buffer agent; according to the fermentation stages, the fermentation conditions are accurately controlled stage by stage; an online monitoring system is established, key parameters in the fermentation process are monitored in real time, a neural network model is established, and the stability and high efficiency of the fermentation process are ensured. According to the method for improving the fermentation stability of the vitamin B12, a diversified carbohydrate mixture is used as a carbon source, a nitrogen source combination is optimized, key elements are accurately added, a specific buffer system is constructed, and an online monitoring system and a neural network model are established for big data analysis and intelligent prediction; the synthesis of vitamin B12 is promoted, and the stability and high efficiency of the fermentation process are ensured.

The microorganism strain Pediococcus acidilactici TAK 589 CoccobEst is a new antimicrobial and antioxidant probiotic, CoccobEst inhibits the growth of the enteropathogens L. monocytogenes, E. coli, S. enteritidis, S. typhimurium, S. sonnei, S. aureus, E. faecalis and C. sakazakii, but does not inhibit lactobacilli of natural intestinal microbiota. The strain's antimicrobial properties are enhanced by periplasmic peptidoglycan hydrolases with proteolytic activity. CoccobEst is used as a zootechnieai feed additive to prevent bacterial infections in the gastrointestinal tract of farm animals and pets and to prevent and reduce diarrhea. CoccobEst has highly active antioxidant defense system, increasing the redox activity of glutathione and reducing oxidative stress in cells. Therefore, CoccobEst is used as a food supplement or functional food for humans to prevent bacterial infections of the gastrointestinal tract and to prevent and reduce oxidative stress. In addition, CoccobEst is used as a starter culture in fermentation of vegetables.

The invention relates to the field of microorganisms, and discloses a stress-resistant heterotrophic nitrification-aerobic denitrification nitrogen removal bacterium and application thereof. The pseudomonas is named as H1, the microorganism classification name of the pseudomonas is Pseudomonas sihuiensis, the preservation number of the pseudomonas is CGMCC (China General Microbiological Culture Collection Center) NO: 33481, the preservation date of the pseudomonas is January 20, 2025, and the preservation place of the pseudomonas is China General Microbiological Culture Collection Center. The pseudomonas H1 disclosed by the invention can still realize efficient denitrification in a heterotrophic nitrification-aerobic denitrification way in a high-salt and high-nitrogen water body environment containing toxic pollutants with benzene ring functional groups, so that the pseudomonas H1 has great application potential in biological treatment of nitrogen-containing wastewater.

The invention discloses lactobacillus crispatus CML521 and application thereof, and relates to the technical field of microorganisms, and the lactobacillus crispatus CML521 is technically characterized in that the lactobacillus crispatus CML521 is obtained through separation and culture from poultry craw contents, and the lactobacillus crispatus CML521 has remarkable biological characteristics and can be effectively used for preparing medicines for treating poultry candidiasis; the discovery of the lactobacillus crispatus CML521 provides a brand new treatment thought and method for poultry to treat candidiasis, and the newly discovered microbial strain not only can help poultry to resist the infringement of candidiasis, but also is expected to play an important role in the veterinary medicine field, and provides guarantee for poultry health.

The present invention provides a multifunctional Paenibacillus terrae and its application, relating to the technical field of microorganisms and their applications. The multifunctional Paenibacillus terrae is the Paenibacillus terrae strain KY834, which is deposited in the General Microbiological Center of the China Committee for Culture Collection of Microorganisms, and the deposit number is CGMCC No. 24708. From a large number of microbial strains, the present invention obtained a strain KY834 with the ability to kill the pathogens of apple leaf spot disease and pear black spot disease through high-throughput screening. Further research found that this strain has broad-spectrum bactericidal activity against the pathogens of other plant diseases, and also has the functions of killing algae and activating silicon, showing good application prospects in the biological control of plant (especially fruit tree) diseases.