Bacterial strain for reducing vomitoxin function of wheat and corn and application of bacterial strain

Abstract

The invention relates to the technical field of fertilizers, and particularly discloses a bacterial strain capable of reducing the function of vomitoxin of wheat and corn and application of the bacterial strain, the bacterial strain capable of reducing the function of vomitoxin of wheat and corn is applied to preparation of a fertilizer, 10%-90% of bacillus velezensis XNYF-1 fermentation liquor (A liquor) and 10%-90% of pseudomonas fluorescens XNYF-2 fermentation liquor (B liquor) are mixed to prepare AB mother liquor, and the AB mother liquor is used for preparing the fertilizer. The XNYF-1 and the XNYF-2 are two functional microbial strains which are subjected to screening mutagenesis and can be used for producing chitinase, iturin, various phenazine derivatives and biological surfactin respectively, and the XNYF-1 and the XNYF-2 can be used for producing the chitinase, the iturin, the various phenazine derivatives and the biological surfactin respectively. And the metabolite has a good biocontrol effect.

Description

Technical Field

[0001] The present invention belongs to the technical field of fertilizers, and particularly relates to a strain with the function of reducing vomitoxin in wheat and corn and its application. Background Art

[0002] Vomitoxin (deoxynivalenol; 3 - acetyldeoxynivalenol and 15 - deoxynivalenol), this toxin was first discovered in a fusarium head blight barley poisoning incident in Kagawa Prefecture, Japan in 1970. In 1972, Morooka et al. in Japan first isolated this toxin from fusarium head blight barley. Yoshizawa et al. elucidated the structure of this new mycotoxin and named it 4 - deoxynivalenol (DON). In 1973, Vesonder et al. isolated the same chemical substance from fusarium - contaminated corn in the United States. Because this substance can cause pigs to have vomiting symptoms, it was named vomitoxin. Subsequently, it was successively found that this substance can be isolated from the fusarium head blight ears of barley and wheat, and from fusarium - contaminated corn. Vomitoxin has become a common mycotoxin in food and exists widely in nature.

[0003] Since vomitoxin has high cytotoxic and immunosuppressive properties, it poses a threat to the health of humans and animals, especially having an obvious impact on immune function. Depending on the dose and exposure time of DON, it can cause immunosuppression or immunostimulation. When humans ingest food contaminated with DON, it will cause acute poisoning symptoms such as anorexia, vomiting, diarrhea, fever, unsteadiness, and slow reaction. In severe cases, it can damage the hematopoietic system and cause death.

[0004] Vomitoxin (VT) is an important toxin among more than 150 trichothecenes produced by fungi such as Fusarium, Cephalosporium, Rhizoctonia, and Trichoderma. It has a strong immunosuppressive function. The typical symptoms it causes are reduced feed intake and anorexia. It is a feed toxin that seriously affects the livestock and poultry breeding industry. This toxin enters food and feed through contaminated raw materials such as wheat, barley, and corn. After livestock and poultry consume contaminated feed, a small amount of vomitoxin enters milk, meat, and eggs, thus indirectly affecting human health. Because of its wide presence in grains and their products, it has attracted great attention worldwide. Each country has formulated corresponding control standards and taken risk control and process traceability for the entire chain of grain production and processing, from field planting, harvesting, storage to processing and circulation. Field planting is the source.

[0005] Domestic and international limited standards for vomitoxin. Currently, 37 countries around the world have relevant limited standards for vomitoxin in food or grains. The national standard GB2761-2011 in China stipulates that the limited standard for vomitoxin DON in grains and grain products (including corn, corn flour, barley, wheat, oatmeal, and wheat flour) is 1000 μg / kg. The FDA (Food and Drug Administration) in the United States stipulates that the safety standard for vomitoxin DON in food is 1 mg / kg. When the content of vomitoxin DON exceeds 1 mg / kg, it will cause harm to the health of humans and livestock. At the same time, the United States has set that the allowable limit of vomitoxin DON in wheat and wheat products for feed use shall not exceed 4 mg / kg, while the limited standard for vomitoxin DON set by the European Union is relatively stricter, and the allowable limit in cereal flour and corn flour is ≤ 0.75 mg / kg.

[0006] Field planting is the first stage of vomitoxin production. The scab of wheat and barley, and the ear rot of corn are basically considered as crop diseases caused by Fusarium. Affected by climatic conditions such as temperature, humidity, drought, precipitation, etc., especially when wheat encounters wet and cold weather during the flowering and heading stage, the scab will prevail. The disease ear rate in a major epidemic year reaches 5% - 15%; even more seriously, the occurrence of pests will promote the spread of Fusarium and aggravate the severity of wheat scab and corn ear rot; in addition, the terrain can also affect the production of Fusarium toxins. For example, the differences between the mountaintop and the depression lead to uneven spatial distribution of soil moisture and air humidity, and humidity is an important factor affecting the production of Fusarium toxins on the ear of grain, and this influence has been confirmed by research.

[0007] Grain harvesting and storage are the second and third stages of toxin production. During the grain harvesting period, if it happens to encounter continuous rainy weather, the temperature and humidity are just suitable for the germination and growth of pathogenic Fusarium, and the toxin content in the grain will naturally increase. In some areas of Henan, Jiangsu, Hubei, Anhui and other provinces in 2009 and 2010 in China, when harvesting, it encountered continuous rainy weather, and the proportion of over-standard wheat was close to 30% or higher. The storage stage is also very important. Controlling the moisture content of stored grain is an important way to prevent mildew and the main method to prevent toxin production. Of course, there are also the humidity and temperature in the storage environment space.

[0008] As an important toxin in grains and their products, deoxynivalenol (DON) has attracted worldwide attention. In China's traditional eating habits, the proportion of grains is much higher than that in the West, making the harm of deoxynivalenol more prominent. How to reduce the toxin content in grains and their products is the focus of research and development by relevant technical departments, and many achievements have been made, including physical, biological, and chemical methods. However, the implementation of these technical methods is basically in the stages of grain storage and processing, which are remedial measures when the toxin has already been produced. With the rapid development of modern agricultural production technology, the input and popularization of modern agricultural machinery, and the continuous improvement of harvesting, dehydration drying, and storage conditions, the second and third stages of toxin production will gradually weaken, and the first stage of toxin production, the planting process, will become the main target.

[0009] The present invention uses fertilizers, which are important agricultural supplies in agricultural production, as a carrier, and integrates modern biotechnology into the fertilizer production process, so that the fertilizer product has the effect of inhibiting the germination and reproduction of field pathogenic bacteria, reducing the density of field pathogenic bacteria, effectively reducing the related diseases caused by Fusarium in the planting and production process, and thus reducing the production of deoxynivalenol (DON). Summary of the Invention

[0010] The purpose of the present invention is to provide a strain with the function of reducing deoxynivalenol in wheat and corn and its application, so as to solve the problems raised in the above background technology.

[0011] To achieve the above purpose, the present invention provides the following technical solutions:

[0012] A strain with the function of reducing deoxynivalenol in wheat and corn, the strain is Bacillus velezensis, and the preservation name of Bacillus velezensis is Bacillus velezensis XNYF-1, and the preservation number is: ACCC64130.

[0013] Preferably, the strain with the function of reducing deoxynivalenol in wheat and corn includes Bacillus velezensis and Pseudomonas fluorescens XNYF-2.

[0014] The Pseudomonas fluorescens was isolated from the healthy tomato roots of Bohai Fruit and Vegetable Planting Base in the suburbs of Shanghai, and obtained through multiple mutageneses. It was identified as Pseudomonas fluorescens by a professional institution, and the company's strain bank number is XNYF-2.

[0015] Preferably, the application of the strain with the function of reducing deoxynivalenol in wheat and corn in the preparation of organic fertilizers is characterized in that: a mixed solution AB masterbatch of 10% - 90% of the fermentation broth A of Bacillus velezensis XNYF-1 and 10% - 90% of the fermentation broth B of Pseudomonas fluorescens XNYF-2 is added to the raw materials of decomposed organic fertilizers and humic acids to make organic fertilizers.

[0016] Preferably, a mixed liquid AB masterbatch composed of 10% - 90% of the fermentation broth of Bacillus velezensis XNYF-1 (liquid A) and 10% - 90% of the fermentation broth of Pseudomonas fluorescens XNYF-2 (liquid B) is added with inorganic mineral nutrients and organic fillers, and an organic-inorganic compound fertilizer is produced through a spraying granulation process.

[0017] Preferably, the fermentation conditions for the liquid fermentation of Bacillus velezensis XNYF-1 are as follows: the inoculation amount is 1% - 5%, the temperature is controlled at 33 - 34 °C, the stirring speed is 100 revolutions per minute, the ventilation volume-air liquid ratio is 0.5 - 0.8:1, and the pH value reaches 7.2 - 7.5. After culturing at 28 °C for 46 hours, it is washed down with physiological saline for standby. The fermentation conditions for the liquid fermentation of Pseudomonas fluorescens XNYF-2 fermentation broth are as follows: the temperature is 28 °C, the stirring speed is 130 rpm, the ventilation volume-air liquid ratio is 0.6:1, and it is cultured for 12 hours. The (liquid A) and (liquid B) are mixed in different proportions to form (liquid AB), and the combinations of liquid AB include 4:6 or 3:7.

[0018] Preferably, the matured organic fertilizer includes various raw materials that meet the requirements for making organic fertilizers, such as agricultural waste, livestock and poultry manure, industrial waste, domestic waste, urban sludge, and biogas slurry and biogas residue.

[0019] Preferably, the organic fillers of the organic-inorganic compound fertilizer include humic acid, various industrial waste residues, cakes, and meals, as well as agricultural waste that meets the requirements of the spraying granulation process.

[0020] Preferably, the mineral nutrients of the organic-inorganic compound fertilizer include ammonium salts, urea; phosphates, potassium salts, and medium and trace elements such as calcium, magnesium, silicon, copper, iron, manganese, zinc, boron, and molybdenum.

[0021] Strains with the function of reducing deoxynivalenol in wheat and corn are applied in the prepared fertilizers on food crops including wheat, corn, barley, oats, and sorghum.

[0022] Among them, the present invention

[0023] Other components of the fertilizer: including organic fertilizer, organic matter, and mineral nutrients. The organic fertilizer can be purchased externally or made by self-composting. The organic matter can adopt industrial by-products and leftovers such as lignite, peat, and biochar, and the mineral nutrients are purchased from the agricultural materials market.

[0024] Product fertilizer production

[0025] Mixing of liquid AB: According to the above two microbial fermentation broths (liquid A) and (liquid B), they are mixed in different proportions to form (liquid AB), and the combinations of liquid AB can be 4:6 or 3:7.

[0026] Dosage: The dosage of (AB liquid) in the fertilizer varies from 5% to 50% of the solid content of the fertilizer.

[0027] Organic fertilizer production process

[0028] Conventional process: Basically follow the technological route of pretreatment, primary fermentation, secondary aging, and several post-treatment steps. In the technical implementation of the present invention, (AB liquid) is added in the secondary aging stage and evenly sprayed on the aging organic fertilizer during turning and throwing in the form of high-pressure spraying. Subsequently, it is dried by sunning, pulverized, and screened to make organic fertilizer powder, or it can be made into organic fertilizer granules by extrusion or disc granulation.

[0029] Using finished or semi-finished organic fertilizer as raw material: The secondary aging process can be repeated, and the manufacturing process is the same as above.

[0030] Production of organic-inorganic compound fertilizer

[0031] Product advantages: Organic-inorganic compound fertilizer is an advanced and high-end fertilizer product that scientifically and reasonably integrates organic nutrients and inorganic nutrients, overcomes the shortage of mineral nutrients in single organic fertilizers and the impact of single inorganic fertilizers on soil ecology, and has great potential.

[0032] Granulation process

[0033] Spraying granulation process: A mature process in modern agricultural fertilizer production, which is widely used in the production of inorganic compound fertilizer granules. The product has uniform granules, high strength, is easy to use, has good fertilizer efficiency, and stable quality. The spraying granulation process for organic fertilizer is to mix organic materials with water and then spray them into the granulator using spraying equipment. Under high temperature and high pressure, the moisture of the materials evaporates rapidly, and the organic materials adhere to each other to form granular fertilizers. The smaller the particle size of the organic materials, the smoother the surface of the made fertilizer. Organic-inorganic compound fertilizer is made by fully mixing organic materials and inorganic nutrients to form a slurry and then making fertilizer granules according to this process.

[0034] Requirements for organic materials: It is necessary to select organic raw materials that are easy to grind and break, such as humic acid, lignite, peat soil, amino acid mother liquor, industrial waste, cakes, residues, distiller's grains, and oils. Before batching, they need to be pulverized, filtered, or passed through a Raymond mill to meet the fineness standard required for spraying granulation.

[0035] Requirements for inorganic nutrients: Include raw materials such as ammonium salts, urea, nitrates, phosphates, potassium salts, and medium and trace elements that meet agricultural requirements. They are required to have good water solubility, no hard particles that are difficult to dissolve in water, and meet the preparation requirements of the spraying granulation slurry.

[0036] Preparation of spraying slurry

[0037] Composition: Organic materials + inorganic nutrients + (AB solution) + water. The organic matter content and main indicators such as NPK nutrients are calculated and formulated according to the matching models of the GB18877-2009 standard. The requirement indicators of other auxiliary nutrients are added according to the fertilizer design. The addition of (AB solution) can reduce the water consumption during slurry preparation.

[0038] Operation: After measuring each component, they are separately put into the stirring pot, and the water is supplemented according to a solid content of 30%-40%. Start stirring to fully mix the materials of each component. Increase or decrease the water volume or add relevant additives according to the flow state of the slurry to ensure the smooth spraying of the materials. According to the physical and chemical properties of the slurry components, the inlet air temperature during granulation can be adjusted to avoid the decomposition or carbonization of sensitive substances.

[0039] Subsequent processes: The prepared slurry is sent into the nozzle by a self-priming high-pressure pump and atomized by high-pressure air into the granulation and drying cylinder. The granulation and drying cylinder is divided into three chambers: front, middle, and rear, which respectively complete the functions of granulation, classification and return of materials, and drying. The particles coming out of the granulation and drying cylinder enter the cooling cylinder, where they are cooled, screened, inspected, packaged, and stored in the warehouse.

[0040] Compared with the prior art, the beneficial effects of the present invention are:

[0041] Microbial strains with high yields of chitinase, iturin, various phenazine derivatives, and biosurfactin: XNYF-1, XNYF-2.

[0042] Since the main function of this microorganism in the fertilizer is its metabolites, the efficacy of the bio-organic fertilizer or compound microbial fertilizer made from this microorganism is not necessarily stable. Because it colonizes in the soil and the conditions for its propagation and growth are far less good than those in the fermentation tank medium, it is very difficult to obtain metabolites in the natural soil environment. Therefore, adding the fermentation broth with stable metabolites to the fertilizer can ensure the stable efficacy of the fertilizer.

[0043] The survival time of the microorganism in the organic fertilizer is not as long as the retention time of its metabolites in the organic fertilizer; that is to say, the shelf life of the fertilizer made from the microbial metabolites is longer than that of the bio-organic fertilizer or compound microbial fertilizer.

[0044] After repeated tests, the structure of the main functional metabolite is stable and is not affected by the cold, heat, and mechanical operations during the fertilizer production process. The moisture content of the end product is very low, which can ensure a long shelf life. Specific embodiments

[0045] Next, in combination with the embodiments of the present invention, the technical solutions in the embodiments of the present invention will be clearly and completely described. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0046] The test implementation equipment used in the present invention are all conventional production equipment, including instruments for laboratory biological cultivation, small and medium-scale pilot fermentation equipment, and a spraying production process for fertilizer production. There are no special equipment process requirements, and the raw materials are all commercially available products in the market.

[0047] The following explanations are made for the raw and auxiliary materials and related terms in the specific implementation of the present invention.

[0048] The fermentation broth (solution A) of strain XNYF-1 is provided by a biological products company in Pucheng, Fujian for fermentation contract manufacturing. The capacity of the conventional fermentation tank is 20 cubic meters, the filling coefficient is 0.6 - 0.7, the output of the primary fermentation broth is 12 - 14 tons, and after fermentation, it is inactivated and preserved with preservatives for standby.

[0049] The fermentation broth (solution B) of strain XNYF-2 is fermented by a biological products company in Shanghai. The capacity of the fermentation tank equipment is 20 cubic meters, the output of the primary fermentation broth is 12 - 14 tons, and after fermentation, it is inactivated and preserved with preservatives for standby.

[0050] The specific implementation method is to produce an organic-inorganic compound fertilizer, which is a new type of agricultural base fertilizer expected to replace chemical fertilizers. The organic matter is selected from the by-products of a humic acid production enterprise in Shanxi and the amino acid waste mother liquor of Hebei Xinshengyuan, and mineral nutrients such as nitrogen, phosphorus, and potassium purchased from the market are added to produce organic-inorganic compound fertilizers with nutrient structures of 15-8-10 and 20-8-5 for planting tests of field crops such as wheat and corn.

[0051] Example 1:

[0052] Technicians carried out the first trial production in Qiji Ecological Organic Fertilizer Co., Ltd. in Lingshi County, Shanxi. Since the company has a set of medium and small-scale fertilizer spraying granulation equipment, which meets the conditions for the implementation of the present invention, the front-end and back-end treatments of the raw and auxiliary materials are relatively perfect.

[0053] In this implementation, the ratio of (solution A) to (solution B) is 50:50. The AB mixed solution accounts for 20% of the spraying slurry, the amino acid mother liquor accounts for 20%, and other raw and auxiliary materials are added according to the nutrient structure with a final moisture content of 3% of the fertilizer, and the organic matter content is ensured to meet the standard requirements.

[0054] After detailed and careful planning, on May 13, 2021, a shift of trial production was carried out on the spraying granulation process line of Lingshi County Qiji Ecological Organic Fertilizer Co., Ltd. The product model was 15-8-10. The product quality indicators met the design requirements, and the shift output was about 20 tons.

[0055] The test production process is carried out strictly in accordance with the process design index parameters.

[0056] Embodiment 2:

[0057] Based on the implementation of Case 1, in October of the same year, the second trial production was carried out at Shanxi Lingshi County Qiji Ecological Organic Fertilizer Co., Ltd. The nutrient structure of the trial fertilizer was 20-8-5, with a target output of 20 tons.

[0058] The microbial fermentation liquid A and liquid B used are the same batches as those in Example 1, and the mixing ratio is adjusted to (liquid A): (liquid B) = 40:60, the AB mixed liquid accounts for 20% of the spraying liquid, the amino acid mother liquor accounts for 20%, and the output of other raw and auxiliary materials is slightly adjusted, and the calculation method remains unchanged.

[0059] Due to changes in the nutrient structure of the product, the operating parameters of the granulation process were slightly adjusted, and the product technical indicators basically met the design requirements.

[0060] Embodiment 3

[0061] At the test base of the company's headquarters, technicians use a 2-cubic-meter crawler mixing equipment to spray the AB mixture into the purchased mature organic fertilizer during the mixing process. The mixed organic fertilizer continues to be put into the pool for aging, and is regularly turned over to reduce the moisture content.

[0062] When the moisture content reaches the standard of commercial organic fertilizer, it can enter the next procedure, or crush, screen, test and package according to the powder fertilizer; or carry out the crushing, screening and granulation process.

[0063] Because organic fertilizers have a high moisture content and their microbial metabolites are easily degraded, attention should be paid to shelf life, as long-term storage may affect their efficacy.

[0064] Application Case 1:

[0065] In 2021, 100 acres of winter wheat in Dazhouying Village, Longyan Township, Dengzhou City, Nanyang City, Henan Province used the 15-8-10 organic-inorganic compound fertilizer produced by the present invention as base fertilizer, and the 20-8-5 organic-inorganic compound fertilizer as topdressing, and the harvest of the following year achieved the effect of improving quality and increasing yield.

[0066] The fertilizer application plan is as follows

[0067] Winter wheat in Dazhouying Village, Longyan Township, Dengzhou City, Nanyang, Henan-(2021 Fertilizer Application Plan)

[0068]

[0069] The above fertilization plan is for the double reduction project of reducing chemical fertilizers and pesticides in response to the country. The fertilization is a fertilization plan formulated through soil testing and formula fertilization, aiming to achieve a yield not lower than that of traditional fertilization, and the quality toxin of the product is an accompanying detection index.

[0070] The test results of a toxin index, deoxynivalenol, of wheat in this case are as follows:

[0071]

[0072] According to the above detection data of deoxynivalenol, the deoxynivalenol index of wheat using the organic-inorganic compound fertilizer prepared by the present invention as base fertilizer and top dressing is much lower than that of traditional fertilization.

[0073] Application case 2:

[0074] In 2022, spring-sown dryland corn planted by Shanxi Nongyi Biotechnology Co., Ltd. in Chengzi Village, Qingxu County, Shanxi Province. The planting area in the region is nearly 20,000 mu. Among them, 50 mu uses the 20-8-5 organic-inorganic compound fertilizer produced by implementing the present invention as base fertilizer, the control plot uses 20-9-6 organic-inorganic blended fertilizer, and the other plots use high-nutrient compound fertilizers such as 25-10-10 and 22-14-7, with various nutrient structures.

[0075] The fertilization plan for the above case 2 is as follows:

[0076] Spring-sown dryland corn in Chengzi Village, Qingxu County, Shanxi Province - (Fertilization plan in 2022)

[0077]

[0078] The above corn planting is an exploratory planting experiment of Shanxi Nongyi Biotechnology Co., Ltd. in Qingxu County, Shanxi Province, aiming to accumulate effective data for large-scale planting in the next year and at the same time detect relevant indicators of yield and quality.

[0079] The test results of a toxin index, deoxynivalenol, of corn in this case are as follows:

[0080]

[0081] According to the above detection data of deoxynivalenol, the deoxynivalenol index of corn using the organic-inorganic compound fertilizer prepared by the present invention as base fertilizer is also lower than that of organic-inorganic blended fertilizer and traditional fertilization.

[0082] It should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention rather than to limit them. Although the present invention has been described in detail with reference to the preferred embodiments, those of ordinary skill in the art should understand that the technical solutions of the present invention can be modified or equivalently replaced without departing from the spirit and scope of the technical solutions of the present invention, and all of them should be covered by the scope of the claims of the present invention.

Claims

1. A strain that reduces the vomitoxin function of wheat and corn, characterized in that: The strain is Bacillus velezensis, the preservation name of the Bacillus velezensis is Bacillus velezensis XNYF-1, and the preservation number is ACCC64130.

2. The strain for reducing the vomitoxin function of wheat and corn according to claim 1, characterized in that: The strains for reducing the vomitoxin function of wheat and corn include Bacillus Velez and Pseudomonas fluorescens XNYF-2.

3. The use of the strain for reducing the vomitoxin function of wheat and corn according to any one of claims 1-2 in the preparation of organic fertilizer, characterized in that: The raw material of organic fertilizer is prepared by adding decomposed organic fertilizer and humic acid to a mixed liquid AB masterbatch of 10% to 90% of Bacillus Velez XNYF-1 fermentation liquid (A liquid) and 10% to 90% of Pseudomonas fluorescens XNYF-2 fermentation liquid (B liquid), and adding decomposed organic fertilizer and humic acid.

4. Use of the strain for reducing the vomitoxin function of wheat and corn according to any one of claims 1-2 in the preparation of organic and inorganic fertilizers, characterized in that: The organic-inorganic compound fertilizer is prepared by using a mixed solution AB masterbatch of 10% to 90% of Bacillus Velez XNYF-1 fermentation solution (A solution) and 10% to 90% of Pseudomonas fluorescens XNYF-2 fermentation solution (B solution), adding inorganic mineral nutrients and organic fillers, and performing a spraying granulation process.

5. The use of the strain for reducing the vomitoxin function of wheat and corn in the preparation of fertilizer according to claim 2, characterized in that: The fermentation conditions for liquid fermentation production of Bacillus velezensis XNYF-1 are as follows: an inoculation amount of 1%-5%, a temperature control of 33-34 degrees, a stirring speed of 100 rpm, a ventilation volume gas-liquid ratio of 0.5-0.8:1, and a pH value of 7.2-7.

5. After culturing at 28 degrees for 46 hours, the fermentation liquid is washed with physiological saline for standby use. The fermentation conditions for liquid fermentation production of Pseudomonas fluorescens XNYF-2 fermentation liquid are as follows: a temperature of 28 degrees, a stirring speed of 130 rpm, a ventilation volume gas-liquid ratio of 0.6:1, and culturing for 12 hours. The (A liquid) and (B liquid) are mixed in different proportions to form (AB liquid), and the combination of AB liquid includes 4:6 or 3:

7.

6. The use of the strain for reducing the vomitoxin function of wheat and corn in the preparation of organic fertilizer according to claim 3, characterized in that: The decomposed organic fertilizer includes agricultural waste, livestock and poultry excrement, industrial waste, domestic garbage, urban sludge, biogas slurry and biogas residue and other raw materials suitable for making organic fertilizer.

7. The use of the strain for reducing the vomitoxin function of wheat and corn in the preparation of organic and inorganic fertilizers according to claim 4, characterized in that: The organic filler of the organic-inorganic compound fertilizer includes humic acid, various industrial waste dregs, residues, cakes, dregs and agricultural waste that meets the requirements of the spray granulation process.

8. The use of the strain for reducing the vomitoxin function of wheat and corn in the preparation of organic and inorganic fertilizers according to claim 4, characterized in that: The mineral nutrients of the organic-inorganic compound fertilizer include ammonium salt and urea; Phosphates, potassium salts, trace elements calcium, magnesium, silicon, copper, iron, manganese, zinc, boron, and molybdenum.

9. The fertilizer prepared by the strain for reducing the vomitoxin function in wheat and corn according to any one of claims 1-2 is applied to food crops including wheat, corn, barley, oats and sorghum.

Images