76 results about "Rosa chinensis" patented technology

The invention discloses application of a Chinese rose gene RhMYB1 in regulation of Chinese rose axillary bud germination. Relates to the technical field of molecular biology, and provides application of a product for inhibiting expression of an RhMYB1 gene or transcriptional translation protein of the RhMYB1 gene in regulation of axillary bud germination of Chinese roses, and a nucleotide sequence of the RhMYB1 gene is shown as SEQ ID NO.1. According to expression results of the RhMYB1 gene in buds at different sites of Chinese roses, it is found that the expression quantity of the RhMYB1 gene in upper buds (namely active buds) is high. The RhMYB1 gene in the axillary bud is instantaneously silenced, the silent RhMYB1 gene is found to significantly inhibit the germination and growth of the axillary bud, and the overexpression RhMYB1 can promote the growth of the axillary bud.

The invention provides a multi-variety fusion grafting control method for Chinese rose flowers, a server, a medium and a product, and relates to the technical field of Chinese rose fusion control application. The method comprises the following steps: firstly, acquiring a plurality of candidate grafting schemes, collecting rootstock monitoring data and to-be-scion Chinese rose plant information in combination with the schemes, inputting the rootstock monitoring data and the to-be-scion Chinese rose plant information into a growth feature matching prediction model formed by deep learning training according to historical grafting records, scion variety features, grafting results and expert experience, and performing scion grafting. Calculating the growth characteristic matching degree of the to-be-scion and the rootstock, and screening out candidate to-be-scion plant information according to the growth characteristic matching degree; combining the rootstock data and the candidate information to obtain a growth condition score through a fusion growth prediction model, and selecting a scheme with the highest score as an optimal scheme; and finally generating guidance data and sending the guidance data to a user side. By implementing the method, the Chinese rose growth characteristic matching degree can be accurately determined, so that the combination with high matching degree is screened out, the grafting success rate is greatly improved, and grafting failures caused by compatibility problems are reduced.

The invention discloses a Chinese rose vase life whole genome SNP molecular marker combination and application thereof, the SNP locus combination comprises 1679 SNP markers significantly associated with Chinese rose vase life, and the SNP markers are uniformly distributed on 14 chromosomes of Chinese rose. The SNP loci are obtained through genome-wide association analysis and screening, a genome-wide selection model constructed by the SNP loci can accurately predict the vase life of the Chinese rose, and the prediction accuracy can reach 0.710 or above. The invention also relates to specific probes designed aiming at the SNP loci and a liquid phase breeding chip containing the probes, which can be used for rapidly and efficiently screening individuals with excellent vase life characters in a Chinese rose seed or seedling stage, so that the breeding period is remarkably shortened, the breeding efficiency is improved, and the breeding cost is reduced. Powerful technical support is provided for molecular breeding and variety improvement of the Chinese roses.

The invention discloses a China rose inflorescence whole genome SNP molecular marker combination and application thereof. A traditional breeding method for improving China rose inflorescence characters is time-consuming and labor-consuming, and ideal plants are difficult to obtain quickly. The SNP molecular marker technology is utilized, 358 Chinese rose materials and inflorescence phenotype data are analyzed through whole genome association, 1574 SNP loci remarkably related to inflorescence are screened out, a whole genome selection model with the prediction accuracy larger than 0.743 is constructed, and inflorescence characters can be effectively predicted. Meanwhile, a corresponding probe is synthesized to be used for capturing and sequencing, the capturing efficiency is high, the coverage depth is good, the SNP sites related to the rosa chinensis inflorescence can be accurately detected, an efficient technical means is provided for rosa chinensis genetic improvement and new variety cultivation, and the development of the rosa chinensis industry is assisted.

The invention discloses a China rose flowering period regulation and control planting device, and relates to the field of China rose planting devices.The China rose flowering period regulation and control planting device comprises clamping devices, supporting spokes and an integrated assembly, the arch-shaped supporting spokes are arranged at the tops of the clamping devices, and the multiple clamping devices and the supporting spokes are evenly installed on the edge of a flowerpot to form a cage-shaped structure; a temperature control device, an illumination device and a spraying device are installed at the bottom of the integrated assembly, and the cage-shaped structure is wrapped with an isolation cover. A plurality of clamping devices are installed at the edge of a flowerpot, supporting spokes at the tops of the clamping devices form a cage-shaped structure, and the outer surface of the cage-shaped structure is coated with an isolation cover, so that Chinese roses in the flowerpot are isolated from the outside, the flowering phase is convenient to regulate and control, meanwhile, the device can adapt to most flowerpot sizes due to the separated design, installation is convenient, and the cost is low. The method is suitable for domestic Chinese rose planting; meanwhile, a player can conveniently define the water and fertilizer applying amount and the illumination duration and conduct temperature management in the regulation and control period, the planting fun of the player is increased, and the rosa chinensis sales volume is promoted.

The invention discloses a China rose fragrance whole genome SNP molecular marker combination and application thereof, 2068 SNP markers significantly related to fragrance are screened out by analyzing 358 parts of China rose re-sequencing materials and flower fragrance phenotype data, and a whole genome selection model is constructed. The method can accurately predict the fragrance character of the China rose, improve the breeding efficiency and accuracy, shorten the breeding period and reduce the cost. Meanwhile, a new direction is provided for China rose fragrance improvement and breeding, the method has reference value for character improvement of other horticultural crops, has efficient and stable capture efficiency, and can be used for subsequent sample detection and evaluation.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker combination based on Chinese rose germplasm resource typing as well as an identification method and application thereof, 5138 key sites are screened out by analyzing re-sequencing data of 126 parts of rose germplasm resources, and the SNP molecular marker combination based on Chinese rose germplasm resource typing is obtained by combining phylogenetic analysis, population structure analysis and principal component analysis. And an important basis is provided for genetic composition identification of Chinese rose germplasm resources. The method can accurately predict the genetic background of the Chinese rose germplasm resources, and provides a scientific basis for breeding work, so that the breeding efficiency and accuracy are improved, the breeding period is shortened, and the cost is reduced. Meanwhile, a new direction is provided for distinguishing the genetic composition and breeding of the Chinese rose germplasm resources, and the method has reference value for identifying the genetic composition of other horticultural crop traits. In addition, the designed liquid-phase breeding chip has efficient and stable capture efficiency, and can be widely applied to detection and evaluation of large-batch samples.

The invention discloses a Chinese rose petal fold whole genome SNP (Single Nucleotide Polymorphism) molecular marker combination and application thereof. 968 SNP markers obviously related to petal fold traits are screened out and distributed on 14 chromosomes by analyzing 358 parts of Chinese rose re-sequencing data and utilizing a plurality of software models to carry out whole genome association analysis. A liquid-phase gene chip is designed on the basis of the SNP loci, and plants with ideal petal folding characters can be rapidly screened out through DNA detection in the Chinese rose seedling stage. The liquid phase breeding chip has the advantages of high throughput detection, low cost, shortened breeding period and the like, can simultaneously detect a large number of SNP sites and reduce the cost, can predict future performance of characters in a seed or seedling stage, greatly shortens the breeding period, accelerates cultivation of a new Chinese rose variety with a unique petal rolling character, and has a wide application prospect. The requirements of the market for novel flower types are met, the ornamental value and market competitiveness of the Chinese rose variety are improved, and the development of the flower industry is promoted.

The invention discloses a Chinese rose petal diameter whole genome SNP molecular marker combination and application thereof. The SNP molecular marker combination comprises 4669 SNP sites distributed on 14 chromosomes of a Chinese rose; by analyzing 358 rosa chinensis re-sequencing data and through the steps of GWAS association analysis and the like, an SNP site combination closely associated with the petal diameter is accurately screened out, a high-specificity probe and a chip are designed and developed, a complete application system is constructed, and links of parent analysis, offspring screening, new variety identification and the like are covered. The technology overcomes the defects of blindness, long period and the like of the existing breeding method, realizes high-throughput and high-sensitivity detection, improves the breeding accuracy and efficiency, shortens the breeding period, meets the market demand, provides an accurate judgment basis for Chinese rose variety identification, and promotes the modernization development of the Chinese rose industry.

The invention discloses a Chinese rose petal outer edge notch whole genome SNP molecular marker combination and application thereof, and aims to overcome the defects in the prior art, 879 SNP markers significantly related to petal outer edge notch are identified by fusing 358 Chinese rose whole genome re-sequencing data and petal outer edge notch phenotype correlation analysis and applying a multi-model combined screening technology, and the SNP markers are used for identifying the petal outer edge notch. And a whole genome selection model with high prediction accuracy is established. The markers are distributed on 14 chromosomes, high-throughput and rapid detection can be achieved, the detection period is shortened, the petal wave character is directly predicted through seedling genotype data, and the breeding process is accelerated. The method has the advantages of low cost, high throughput and industrialization potential, provides key data support for Chinese rose petal outer edge notch genetic law analysis and molecular marker assisted breeding, and significantly improves the breeding efficiency and precision.

The invention provides a plant extract with triple effects of inhibiting bacteria, resisting oxidation and relieving itching, a composition and a preparation method and application of the plant extract and the composition, and relates to the technical field of daily chemical products. A comprises a myrobalan fruit extract; b is a plum fruit extract, or at least one of a rose flower extract, a Chinese rose flower extract or a tribulus terrestris fruit extract and the plum fruit extract; when b is the plum fruit extract, the weight ratio of a to b is (2-4): 1; when b is at least one of the rose extract, the China rose extract or the tribulus terrestris fruit extract and the plum fruit extract, the weight ratio of a to b is 1: 1. The composition can play good antibacterial, antioxidant and antipruritic roles and realize a relatively strong synergistic effect, so that a better effect can be achieved when the composition is applied to a skin care product. The technical problem that in the prior art, a plant extract capable of simultaneously achieving the effects of inhibiting bacteria, resisting oxidation and relieving itching is lacked is solved.

According to the long-acting sterile ornamental and regeneration integrated culture method for the in-vitro Chinese rose buds, the pollution risk is low, and a fundamental guarantee is provided for long-term sterile culture of the Chinese rose bud explants through the broad-spectrum and lasting antibacterial effect of the in-vitro Chinese rose buds. Under the system, the complete period from flowering to decline of the Chinese rose bud explant is completed by means of the slow-release nutrition, and the viewing period of the cut Chinese rose flower is remarkably prolonged. In addition, after the ornamental period is finished, the cut Chinese rose flowers also have complete transplanting survival ability, and the transformation from disposable consumption to sustainable cultivation is realized. The scheme has the industrialization advantages of being short in production period, high in stability and low in maintenance cost, is suitable for all-season regional application, remarkably improves the ornamental value and practicability of the product, and provides an innovative product for the fields of home decoration, high-end gift presentation, medical health care, education and science popularization and the like.

The invention relates to the technical field of microbial fermentation, and discloses a fermentation composition with a whitening effect and a preparation method and application thereof. According to the application, Chinese rose, a sodium glutamate culture medium and sprouted mung beans are respectively used as fermentation objects, and are fermented by selecting plant lactobacillus and bacillus subtilis to respectively obtain a Chinese rose fermented product, a glutamic acid fermented product and a mung bean sprout fermented product; compared with the prior art, the arbutin fermented product has the advantages that the arbutin fermented product shows more excellent monophenol enzyme inhibition rate and diphenol enzyme inhibition rate which are superior to those of arbutin, particularly, the effect is optimal when the three fermented products are compounded, and a theoretical basis is provided for development and application of whitening cosmetic raw materials and products.

The invention provides a medicinal and edible traditional Chinese medicine composition as well as a preparation method and application thereof, and relates to the technical field of traditional Chinese medicine compositions. Comprising the following components in parts by weight: 3-5 parts of spina date seed, 3-5 parts of liquorice, 5-10 parts of rehmannia, 5-10 parts of lily, 5-10 parts of poria cocos, 10-30 parts of lotus seed, 3-5 parts of China rose, 10-20 parts of lucid ganoderma, 10-20 parts of raspberry, 10-20 parts of mulberry, 10-20 parts of fingered citron and 5-10 parts of American ginseng. The raw material medicines and the dosage thereof supplement each other and cooperate with each other, so that the effects of soothing the nerves and beautifying are effectively achieved, clinical symptoms are effectively improved, and the traditional Chinese medicine composition has the characteristics of remarkable curative effect and few side effects.

The invention discloses an application of an RcHDA19 gene in regulation and control of drought resistance and salt tolerance of Chinese rose, belongs to the technical field of agricultural biological genetic engineering, and discloses the application of the RcHDA19 gene in regulation and control of drought resistance and salt tolerance of Chinese rose, and the nucleotide sequence of the RcHDA19 gene is as shown in SEQ ID NO.1. According to the application, a new choice is provided for promoting the drought resistance and the salt resistance of the rosa chinensis, the RcHDA19 gene is integrated into the rosa chinensis through a silent vector, the tolerance of a rosa chinensis plant to drought and salt stress is improved, and the important application value is achieved for rosa chinensis breeding.

The invention discloses application of a gene RrZFP1 in regulation and control of rose plant flower fragrance, and belongs to the technical field of plant genetic engineering, the nucleotide sequence of the gene RrZFP1 is shown as SEQ ID NO.1, and the amino acid sequence of expression protein of the gene RrZFP1 is shown as SEQ ID NO.2. The RrZFP1 gene of the Fenghua rose is separated, after VIGS in the Chinese rose is silenced, the content of geraniol serving as an important aroma component of a transgenic Chinese rose flower is remarkably reduced, and experiments verify that the RrZFP1 gene can activate RrNUDX1 gene expression of a geraniol synthesis pathway, and indicate that the RrZFP1 is a positive regulation factor of the rose fragrance, and the RrZFP1 gene can be used for preparing the rose essential oil. The important application value is realized in the aspect of regulating and controlling the fragrance of the rosa flowers.

The invention provides an ethylene biosynthesis RhACO4 gene for regulating and controlling the aging of Chinese rose flowers and application of the ethylene biosynthesis RhACO4 gene, and belongs to the technical field of gene engineering. The coding nucleotide sequence of the RhACO4 gene is as shown in SEQ ID NO. 1. According to the invention, the fact that two genotypes (ProRhACO4A and ProRhACO4B) exist in an RhACO4 promoter region in 358 tetraploid Chinese rose populations is found for the first time, and the difference is that whether a section of GA dinucleotide tandem repeat sequence (GA-peak) which is 11 times is contained or not. The ProRhACO4A type carrying GA-peak is significantly related to a lower gene expression level, a lower endogenous ethylene synthesis amount and a longer vase life. The invention provides a key gene target, a molecular marker and a technical path for cultivating a new variety of rosa chinensis with a long flowering period through molecular marker-assisted selection or genetic engineering means.

The invention provides an application of a compound enzyme in preparation of a Chinese rose protoplast, and belongs to the technical field of plant protoplast preparation. According to the compound enzyme disclosed by the invention, the cellulase R-10 and the macerozyme R-10 are compounded for use, and the cellulase R-10 mainly acts on a cellulose component in a cell wall of a Chinese rose leaf, so that a cell wall structure begins to collapse; the macerozyme R-10 can act on components such as pectin in cell walls, and has a synergistic effect with cellulase R-10, so that the cell wall structure is further destroyed, and the release of protoplast is promoted. When the compound enzyme is used for preparing the rosa chinensis protoplast, the high activity of the protoplast is kept, and the number of the obtained protoplast is large. The method disclosed by the invention has outstanding beneficial effects in the aspects of keeping the high activity of the rosa chinensis protoplast and increasing the number of the rosa chinensis protoplast, and opens up a broader way for related research and application of the rosa chinensis protoplast.

The application belongs to the technical field of cosmetics, and discloses a Rosa chinensis / Rhizopus stolonifer fermenting composition and a moisturizing and oil-controlling composition. The preparation method of the Rosa chinensis / Rhizopus stolonifer fermenting composition is as follows: dried Rosa chinensis petals are ground and sieved, inoculated into a fermentation medium, and then inoculated with Lactobacillus pentosus for fermentation culture; the obtained fermentation liquor is inactivated, filtered, concentrated and purified to obtain a Rosa chinensis fermentation product filtrate; then the Rosa chinensis fermentation product filtrate is mixed with a Rhizopus stolonifer fermentation product filtrate to obtain the Rosa chinensis / Rhizopus stolonifer fermenting composition. The moisturizing and oil-controlling composition comprises Hamamelis virginiana extract, Buxus seed extract, lactobionic acid, tea tree essential oil, the Rosa chinensis / Rhizopus stolonifer fermenting composition, supermolecular inclusion suberic acid, a surfactant and water. The composition has the synergistic effects of moisturizing, oil-controlling and skin tightening.

The invention relates to the field of plant genetic engineering, in particular to a rosa chinensis high-temperature-resistant gene RcbHLH59 and application thereof.The rosa chinensis high-temperature-resistant gene RcbHLH59 is identified and cloned to obtain the RcbHLH59 gene, and a CDS sequence and a coded amino acid sequence of the RcbHLH59 gene are obtained; secondly, constructing an overexpression vector and a VIGS silencing vector of the gene, performing agrobacterium-mediated transformation on the Chinese rose, verifying that the gene is a nuclear transcription factor in combination with subcellular localization, and verifying that leaves of the Chinese rose overexpressing the gene are dark green and upright, leaves of the Chinese rose silencing the gene are more serious in yellowing and wilting through a high-temperature stress experiment; according to the rosa chinensis high-temperature-resistant gene RcbHLH59 and the application thereof, eight related genes capable of regulating eight high-temperature response metabolic pathways can be regulated, rosa chinensis heat-resistant gene resources can be enriched, a key target is provided for rosa chinensis high-temperature-resistant molecular breeding, the growth vigor and ornamental value of rosa chinensis at high temperature are effectively improved, and popularization and application of the rosa chinensis in landscaping are promoted.

The invention discloses a Chinese rose small heat shock protein family gene RcHsp20-5 and application thereof in regulation and control of the number of floral organs, and belongs to the field of plant genetic engineering breeding. The RcHsp20-5 gene is separated and cloned from a Chinese rose variety YuLinglong on the basis of a plant gene cloning technology, the nucleotide sequence of the RcHsp20-5 gene is as shown in SEQ ID NO.1, and the coded amino acid sequence is as shown in SEQ ID NO.2. The expression protein of the RcHsp20-5 gene belongs to a CI subfamily. An overexpression vector is constructed, the gene is introduced into arabidopsis thaliana by using a floral immersion method, a high-expression transgenic plant is obtained, and a result shows that the RcHsp20-5 can influence petal symmetry and the number of floral organs of the transgenic arabidopsis thaliana. The Chinese rose RcHsp20-5 provided by the invention may be a potential gene influencing the number of Chinese rose organs, and provides a reference for researching the molecular regulation mechanism of the diversity of ornamental plant flower types.

The invention provides a plant growth regulator for delaying the harvesting time of cut-flower roses as well as a preparation method and application of the plant growth regulator, and belongs to the technical field of fresh cut flower cultivation. The plant growth regulator for delaying the harvesting time of the cut-flower roses comprises the following raw materials: 1-naphthylacetic acid, abscisic acid, cytokinin, a water-soluble fertilizer containing amino acid and monopotassium phosphate. According to the plant growth regulator for delaying the harvest time of the cut-flower roses, provided by the invention, the 1-naphthylacetic acid, the abscisic acid and the cytokinin are compounded together, so that the flower blooming process can be effectively delayed, and the harvest time is delayed. The plant growth regulator takes plant hormones as main raw materials, has the advantages of environmental friendliness, safety, harmlessness, low cost and the like, and further accurately regulates and controls the flower development process of the cut-flower roses by ensuring normal hormone signal transduction and development regulation and control. The growth regulator can effectively delay the harvesting time, powerfully ensures that the cut-flower roses are accurately marketed as required and the quality of the cut-flower roses is improved, and remarkably improves the production benefits and the industrial value.

The invention provides an RcERF98 gene and application of the RcERF98 gene to enhancement of gray mold resistance of Chinese roses, and belongs to the technical field of biological prevention and control. The nucleotide sequence of the RcERF98 gene is as shown in SEQ ID NO: 1. The RcERF98 gene is silent and overexpressed in petals which are good in growth state and free of botrytis cinerea infection of Chinese roses, and research results show that the petals treated by the silent RcERF98 gene are larger in scab area, higher in ion permeability, lower in hydrogen peroxide content and higher in ascorbic acid content, and the resistance of the petals of the Chinese roses to the botrytis cinerea is reduced; the petals treated by the overexpressed RcERF98 gene are smaller in scab area, lower in ion permeability, higher in hydrogen peroxide content and lower in ascorbic acid content, and the resistance of the Chinese rose petals to gray mold is enhanced.

The application discloses RhVPE1 protein and application of a coding gene thereof in regulation and delay of cell programmed death of Chinese rose petal senescence, and an amino acid sequence of the RhVPE1 protein is shown as SEQ ID NO. 3. The application clones a gamma-VPE vacuole processing enzyme RhVPE1 gene from Chinese rose petal, finds that expression of the RhVPE1 gradually rises with petal senescence, and is induced by ethylene, and that the speed of petal from the 5th grade to losing ornamental value is slowed down by silencing of the RhVPE1 gene. In the petal with the RhVPE1 silenced, expression of an aging indicating gene SAG12 is significantly down-regulated. The research result of the application reveals the role of the RhVPE1 in ethylene-regulated petal cell programmed death and senescence, provides a new thought and technical means for regulating plant flower senescence, especially postharvest preservation of Chinese rose fresh-cut flowers, opens up a new flower regulation research field, and has great academic value and important production application prospect.

The utility model provides a Chinese rose cut flower storage and fresh-keeping device, which relates to the technical field of storage and fresh-keeping and comprises a base, moving wheels are mounted on the lower surface of the base, a plurality of mounting blocks are fixedly connected to two sides of the base, and vertical rods are fixedly connected to the upper surfaces of the mounting blocks. Through the arrangement of the placing plates and the inclined surfaces, when the placing frames and China roses leave the conveying belt, the placing frames enter the inclined surfaces on the placing plates, and after the placing frames enter the upper portion of the placing plates, the lifting assembly drives the uppermost placing plate to ascend, so that the second placing plate is aligned with the conveying belt, and the second placing plate receives the placing frames; the Chinese roses after spraying are automatically received and stacked, the inclined plane facilitates standing and water control of the Chinese roses, in the process, the device can be pushed to go to a refrigeration house to prepare for pre-cooling treatment, workers do not need to stack the Chinese roses, the labor intensity of the workers is reduced, and more labor is saved.

The invention provides an efficient rosa chinensis crossbreeding method based on floral organ characters. The method is characterized by comprising the following steps: (1) selection of breeding parents, (2) determination of the breeding parents, (3) planting management of breeding parent variety plants, (4) determination of breeding female parent variety hybrid pollination plants, (5) pollen collection and pollination, and (6) determination of parent varieties with excellent fertility. Finally, the female parent variety and the male parent variety with excellent fertility are subjected to cross breeding, so that the seed setting rate of cross breeding is increased to 90% or above, the cross breeding efficiency is remarkably improved, the breeding cost is reduced, the female parent variety and the male parent variety with excellent fertility can be successfully inherited and improved, and the economic benefit is increased. And an excellent Chinese rose variety with excellent ornamental value and economic value is provided for the market.

The invention discloses a lily cultivation method using Chinese rose waste carbonized products to synergistically reduce application of chemical fertilizers, and relates to the technical field of gardening cultivation, and the method comprises the following specific steps: pretreating collected Chinese rose whole plant waste, removing impurities, cutting, air-drying, and sealing and storing; filling a carbonization furnace with the mixture, carrying out gradient heating carbonization, crushing and sieving to obtain biochar, and sealing and storing the biochar; deeply ploughing the plot, mixing biochar in proportion, and reducing application of chemical fertilizer to prepare a matrix; then disinfecting the lily bulbs, performing field planting according to a certain plant row spacing, and performing thorough watering; drip irrigation, precise topdressing, manual weeding, comprehensive pest control, environment regulation and control and growth index monitoring are adopted in the final growth period; according to the method, the biological carbon prepared from the Chinese rose waste is used for lily cultivation, resource utilization is achieved, pollution is reduced, a cultivation system is constructed by integrating key links, all the links synergistically eliminate growth limitation and improve site conditions, an efficient and stable path is provided for lily cultivation, and high-quality, efficient and sustainable development of the industry is promoted.

Botrytis blight is a major disease in the cultivation of Rosa hybrida, which seriously threatens the quality and yield of cut rose. In order to effectively deal with this disaster stress affecting the yield and quality of rose, 192 pairs of SSR primers were used to amplify the DNA of parents 'Jinmali' and 'Beijinghong' and six randomly selected offspring. Among the parents, 35 pairs of primers showed genetic differences. Further, the DNA of the offspring was amplified using the 35 pairs of primers, and the electrophoresis bands were detected by PAGE electrophoresis and converted into '0, 1 matrix' for genotyping. Through the correlation regression analysis, the optimal subset selection was carried out, and finally three primers related to the resistance to Botrytis blight were successfully screened: Geno-BC-1, Geno-BC-2, Geno-BC-3. The above findings provide an important theoretical basis for molecular marker-assisted breeding of disease resistance in rose, and are expected to promote the early identification of disease-resistant plants, thereby shortening the breeding cycle and improving the breeding efficiency.

The invention discloses a method for obtaining a Chinese rose Norma Jeane transgenic plant, which is characterized in that a somatic embryo induced by immature leaves of Chinese rose Norma Jeane is used as a receptor, and the transgenic Chinese rose plant is finally obtained through induction of the somatic embryo, multiplication culture of a transformed receptor, genetic transformation, co-culture, selective multiplication culture and selective germination culture by an agrobacterium-mediated method. The method is simple to operate and relatively short in culture period, successfully realizes the cultivation of the Chinese rose 'Norma Jeane' transgenic plant, fills the blank that the cultivation of the Chinese rose 'Norma Jeane' transgenic plant cannot be obtained at present, and provides powerful technical support for creating new germplasm of Chinese rose.

The invention provides a disinfection method of an explant for inducing callus of rosa chinensis and a method for inducing the callus, and belongs to the technical field of tissue culture. According to the disinfection method of the explant for inducing the Chinese rose callus, the 75% alcohol aqueous solution and the 2.5% sodium hypochlorite solution are used for combined disinfection of the Chinese rose explant collected in the field, the pollution rate is remarkably reduced to 20% for the problems of high bacterium carrying amount and high disinfection difficulty of a collected material, and the cost is reduced. Meanwhile, high survival rate (80%) and high germination rate (60%) of the explants are ensured. The invention further provides a callus induction method of the disinfected explant, the induction efficiency is improved to 70%, the induction efficiency of the callus is remarkably improved, the induced callus is good in state, light yellow green and loose in texture, and a good material foundation is laid for subsequent tissue culture. The method provided by the invention has the advantages of clear steps, optimized parameters, strong operability and good repeatability.