43 results about "Rosa chinensis" patented technology

The invention discloses application of a Chinese rose gene RhMYB1 in regulation of Chinese rose axillary bud germination. Relates to the technical field of molecular biology, and provides application of a product for inhibiting expression of an RhMYB1 gene or transcriptional translation protein of the RhMYB1 gene in regulation of axillary bud germination of Chinese roses, and a nucleotide sequence of the RhMYB1 gene is shown as SEQ ID NO.1. According to expression results of the RhMYB1 gene in buds at different sites of Chinese roses, it is found that the expression quantity of the RhMYB1 gene in upper buds (namely active buds) is high. The RhMYB1 gene in the axillary bud is instantaneously silenced, the silent RhMYB1 gene is found to significantly inhibit the germination and growth of the axillary bud, and the overexpression RhMYB1 can promote the growth of the axillary bud.

The invention discloses a China rose flowering period regulation and control planting device, and relates to the field of China rose planting devices.The China rose flowering period regulation and control planting device comprises clamping devices, supporting spokes and an integrated assembly, the arch-shaped supporting spokes are arranged at the tops of the clamping devices, and the multiple clamping devices and the supporting spokes are evenly installed on the edge of a flowerpot to form a cage-shaped structure; a temperature control device, an illumination device and a spraying device are installed at the bottom of the integrated assembly, and the cage-shaped structure is wrapped with an isolation cover. A plurality of clamping devices are installed at the edge of a flowerpot, supporting spokes at the tops of the clamping devices form a cage-shaped structure, and the outer surface of the cage-shaped structure is coated with an isolation cover, so that Chinese roses in the flowerpot are isolated from the outside, the flowering phase is convenient to regulate and control, meanwhile, the device can adapt to most flowerpot sizes due to the separated design, installation is convenient, and the cost is low. The method is suitable for domestic Chinese rose planting; meanwhile, a player can conveniently define the water and fertilizer applying amount and the illumination duration and conduct temperature management in the regulation and control period, the planting fun of the player is increased, and the rosa chinensis sales volume is promoted.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker combination based on Chinese rose germplasm resource typing as well as an identification method and application thereof, 5138 key sites are screened out by analyzing re-sequencing data of 126 parts of rose germplasm resources, and the SNP molecular marker combination based on Chinese rose germplasm resource typing is obtained by combining phylogenetic analysis, population structure analysis and principal component analysis. And an important basis is provided for genetic composition identification of Chinese rose germplasm resources. The method can accurately predict the genetic background of the Chinese rose germplasm resources, and provides a scientific basis for breeding work, so that the breeding efficiency and accuracy are improved, the breeding period is shortened, and the cost is reduced. Meanwhile, a new direction is provided for distinguishing the genetic composition and breeding of the Chinese rose germplasm resources, and the method has reference value for identifying the genetic composition of other horticultural crop traits. In addition, the designed liquid-phase breeding chip has efficient and stable capture efficiency, and can be widely applied to detection and evaluation of large-batch samples.

According to the long-acting sterile ornamental and regeneration integrated culture method for the in-vitro Chinese rose buds, the pollution risk is low, and a fundamental guarantee is provided for long-term sterile culture of the Chinese rose bud explants through the broad-spectrum and lasting antibacterial effect of the in-vitro Chinese rose buds. Under the system, the complete period from flowering to decline of the Chinese rose bud explant is completed by means of the slow-release nutrition, and the viewing period of the cut Chinese rose flower is remarkably prolonged. In addition, after the ornamental period is finished, the cut Chinese rose flowers also have complete transplanting survival ability, and the transformation from disposable consumption to sustainable cultivation is realized. The scheme has the industrialization advantages of being short in production period, high in stability and low in maintenance cost, is suitable for all-season regional application, remarkably improves the ornamental value and practicability of the product, and provides an innovative product for the fields of home decoration, high-end gift presentation, medical health care, education and science popularization and the like.

The invention relates to the technical field of microbial fermentation, and discloses a fermentation composition with a whitening effect and a preparation method and application thereof. According to the application, Chinese rose, a sodium glutamate culture medium and sprouted mung beans are respectively used as fermentation objects, and are fermented by selecting plant lactobacillus and bacillus subtilis to respectively obtain a Chinese rose fermented product, a glutamic acid fermented product and a mung bean sprout fermented product; compared with the prior art, the arbutin fermented product has the advantages that the arbutin fermented product shows more excellent monophenol enzyme inhibition rate and diphenol enzyme inhibition rate which are superior to those of arbutin, particularly, the effect is optimal when the three fermented products are compounded, and a theoretical basis is provided for development and application of whitening cosmetic raw materials and products.

The invention discloses application of a gene RrZFP1 in regulation and control of rose plant flower fragrance, and belongs to the technical field of plant genetic engineering, the nucleotide sequence of the gene RrZFP1 is shown as SEQ ID NO.1, and the amino acid sequence of expression protein of the gene RrZFP1 is shown as SEQ ID NO.2. The RrZFP1 gene of the Fenghua rose is separated, after VIGS in the Chinese rose is silenced, the content of geraniol serving as an important aroma component of a transgenic Chinese rose flower is remarkably reduced, and experiments verify that the RrZFP1 gene can activate RrNUDX1 gene expression of a geraniol synthesis pathway, and indicate that the RrZFP1 is a positive regulation factor of the rose fragrance, and the RrZFP1 gene can be used for preparing the rose essential oil. The important application value is realized in the aspect of regulating and controlling the fragrance of the rosa flowers.

The invention provides an ethylene biosynthesis RhACO4 gene for regulating and controlling the aging of Chinese rose flowers and application of the ethylene biosynthesis RhACO4 gene, and belongs to the technical field of gene engineering. The coding nucleotide sequence of the RhACO4 gene is as shown in SEQ ID NO. 1. According to the invention, the fact that two genotypes (ProRhACO4A and ProRhACO4B) exist in an RhACO4 promoter region in 358 tetraploid Chinese rose populations is found for the first time, and the difference is that whether a section of GA dinucleotide tandem repeat sequence (GA-peak) which is 11 times is contained or not. The ProRhACO4A type carrying GA-peak is significantly related to a lower gene expression level, a lower endogenous ethylene synthesis amount and a longer vase life. The invention provides a key gene target, a molecular marker and a technical path for cultivating a new variety of rosa chinensis with a long flowering period through molecular marker-assisted selection or genetic engineering means.

The invention provides an application of a compound enzyme in preparation of a Chinese rose protoplast, and belongs to the technical field of plant protoplast preparation. According to the compound enzyme disclosed by the invention, the cellulase R-10 and the macerozyme R-10 are compounded for use, and the cellulase R-10 mainly acts on a cellulose component in a cell wall of a Chinese rose leaf, so that a cell wall structure begins to collapse; the macerozyme R-10 can act on components such as pectin in cell walls, and has a synergistic effect with cellulase R-10, so that the cell wall structure is further destroyed, and the release of protoplast is promoted. When the compound enzyme is used for preparing the rosa chinensis protoplast, the high activity of the protoplast is kept, and the number of the obtained protoplast is large. The method disclosed by the invention has outstanding beneficial effects in the aspects of keeping the high activity of the rosa chinensis protoplast and increasing the number of the rosa chinensis protoplast, and opens up a broader way for related research and application of the rosa chinensis protoplast.

The invention relates to the field of plant genetic engineering, in particular to a rosa chinensis high-temperature-resistant gene RcbHLH59 and application thereof.The rosa chinensis high-temperature-resistant gene RcbHLH59 is identified and cloned to obtain the RcbHLH59 gene, and a CDS sequence and a coded amino acid sequence of the RcbHLH59 gene are obtained; secondly, constructing an overexpression vector and a VIGS silencing vector of the gene, performing agrobacterium-mediated transformation on the Chinese rose, verifying that the gene is a nuclear transcription factor in combination with subcellular localization, and verifying that leaves of the Chinese rose overexpressing the gene are dark green and upright, leaves of the Chinese rose silencing the gene are more serious in yellowing and wilting through a high-temperature stress experiment; according to the rosa chinensis high-temperature-resistant gene RcbHLH59 and the application thereof, eight related genes capable of regulating eight high-temperature response metabolic pathways can be regulated, rosa chinensis heat-resistant gene resources can be enriched, a key target is provided for rosa chinensis high-temperature-resistant molecular breeding, the growth vigor and ornamental value of rosa chinensis at high temperature are effectively improved, and popularization and application of the rosa chinensis in landscaping are promoted.

The invention provides a plant growth regulator for delaying the harvesting time of cut-flower roses as well as a preparation method and application of the plant growth regulator, and belongs to the technical field of fresh cut flower cultivation. The plant growth regulator for delaying the harvesting time of the cut-flower roses comprises the following raw materials: 1-naphthylacetic acid, abscisic acid, cytokinin, a water-soluble fertilizer containing amino acid and monopotassium phosphate. According to the plant growth regulator for delaying the harvest time of the cut-flower roses, provided by the invention, the 1-naphthylacetic acid, the abscisic acid and the cytokinin are compounded together, so that the flower blooming process can be effectively delayed, and the harvest time is delayed. The plant growth regulator takes plant hormones as main raw materials, has the advantages of environmental friendliness, safety, harmlessness, low cost and the like, and further accurately regulates and controls the flower development process of the cut-flower roses by ensuring normal hormone signal transduction and development regulation and control. The growth regulator can effectively delay the harvesting time, powerfully ensures that the cut-flower roses are accurately marketed as required and the quality of the cut-flower roses is improved, and remarkably improves the production benefits and the industrial value.

The invention provides an RcERF98 gene and application of the RcERF98 gene to enhancement of gray mold resistance of Chinese roses, and belongs to the technical field of biological prevention and control. The nucleotide sequence of the RcERF98 gene is as shown in SEQ ID NO: 1. The RcERF98 gene is silent and overexpressed in petals which are good in growth state and free of botrytis cinerea infection of Chinese roses, and research results show that the petals treated by the silent RcERF98 gene are larger in scab area, higher in ion permeability, lower in hydrogen peroxide content and higher in ascorbic acid content, and the resistance of the petals of the Chinese roses to the botrytis cinerea is reduced; the petals treated by the overexpressed RcERF98 gene are smaller in scab area, lower in ion permeability, higher in hydrogen peroxide content and lower in ascorbic acid content, and the resistance of the Chinese rose petals to gray mold is enhanced.

The utility model provides a Chinese rose cut flower storage and fresh-keeping device, which relates to the technical field of storage and fresh-keeping and comprises a base, moving wheels are mounted on the lower surface of the base, a plurality of mounting blocks are fixedly connected to two sides of the base, and vertical rods are fixedly connected to the upper surfaces of the mounting blocks. Through the arrangement of the placing plates and the inclined surfaces, when the placing frames and China roses leave the conveying belt, the placing frames enter the inclined surfaces on the placing plates, and after the placing frames enter the upper portion of the placing plates, the lifting assembly drives the uppermost placing plate to ascend, so that the second placing plate is aligned with the conveying belt, and the second placing plate receives the placing frames; the Chinese roses after spraying are automatically received and stacked, the inclined plane facilitates standing and water control of the Chinese roses, in the process, the device can be pushed to go to a refrigeration house to prepare for pre-cooling treatment, workers do not need to stack the Chinese roses, the labor intensity of the workers is reduced, and more labor is saved.

The invention discloses a lily cultivation method using Chinese rose waste carbonized products to synergistically reduce application of chemical fertilizers, and relates to the technical field of gardening cultivation, and the method comprises the following specific steps: pretreating collected Chinese rose whole plant waste, removing impurities, cutting, air-drying, and sealing and storing; filling a carbonization furnace with the mixture, carrying out gradient heating carbonization, crushing and sieving to obtain biochar, and sealing and storing the biochar; deeply ploughing the plot, mixing biochar in proportion, and reducing application of chemical fertilizer to prepare a matrix; then disinfecting the lily bulbs, performing field planting according to a certain plant row spacing, and performing thorough watering; drip irrigation, precise topdressing, manual weeding, comprehensive pest control, environment regulation and control and growth index monitoring are adopted in the final growth period; according to the method, the biological carbon prepared from the Chinese rose waste is used for lily cultivation, resource utilization is achieved, pollution is reduced, a cultivation system is constructed by integrating key links, all the links synergistically eliminate growth limitation and improve site conditions, an efficient and stable path is provided for lily cultivation, and high-quality, efficient and sustainable development of the industry is promoted.

Botrytis blight is a major disease in the cultivation of Rosa hybrida, which seriously threatens the quality and yield of cut rose. In order to effectively deal with this disaster stress affecting the yield and quality of rose, 192 pairs of SSR primers were used to amplify the DNA of parents 'Jinmali' and 'Beijinghong' and six randomly selected offspring. Among the parents, 35 pairs of primers showed genetic differences. Further, the DNA of the offspring was amplified using the 35 pairs of primers, and the electrophoresis bands were detected by PAGE electrophoresis and converted into '0, 1 matrix' for genotyping. Through the correlation regression analysis, the optimal subset selection was carried out, and finally three primers related to the resistance to Botrytis blight were successfully screened: Geno-BC-1, Geno-BC-2, Geno-BC-3. The above findings provide an important theoretical basis for molecular marker-assisted breeding of disease resistance in rose, and are expected to promote the early identification of disease-resistant plants, thereby shortening the breeding cycle and improving the breeding efficiency.

The invention discloses a method for obtaining a Chinese rose Norma Jeane transgenic plant, which is characterized in that a somatic embryo induced by immature leaves of Chinese rose Norma Jeane is used as a receptor, and the transgenic Chinese rose plant is finally obtained through induction of the somatic embryo, multiplication culture of a transformed receptor, genetic transformation, co-culture, selective multiplication culture and selective germination culture by an agrobacterium-mediated method. The method is simple to operate and relatively short in culture period, successfully realizes the cultivation of the Chinese rose 'Norma Jeane' transgenic plant, fills the blank that the cultivation of the Chinese rose 'Norma Jeane' transgenic plant cannot be obtained at present, and provides powerful technical support for creating new germplasm of Chinese rose.

The invention provides a disinfection method of an explant for inducing callus of rosa chinensis and a method for inducing the callus, and belongs to the technical field of tissue culture. According to the disinfection method of the explant for inducing the Chinese rose callus, the 75% alcohol aqueous solution and the 2.5% sodium hypochlorite solution are used for combined disinfection of the Chinese rose explant collected in the field, the pollution rate is remarkably reduced to 20% for the problems of high bacterium carrying amount and high disinfection difficulty of a collected material, and the cost is reduced. Meanwhile, high survival rate (80%) and high germination rate (60%) of the explants are ensured. The invention further provides a callus induction method of the disinfected explant, the induction efficiency is improved to 70%, the induction efficiency of the callus is remarkably improved, the induced callus is good in state, light yellow green and loose in texture, and a good material foundation is laid for subsequent tissue culture. The method provided by the invention has the advantages of clear steps, optimized parameters, strong operability and good repeatability.

The application provides application of RcETC3 gene in regulation of flower color of plants. The first aspect of the application provides application of RcETC3 gene in regulation of flower color of plants, and the RcETC3 gene is one of A1) to A3): A1) a nucleic acid molecule with a nucleotide sequence as shown in SEQ ID NO:1; A2) a nucleotide sequence derived from the nucleotide sequence shown in SEQ ID NO:1 through substitution, deletion or addition of one or more nucleotides; and A3) a nucleotide sequence with at least 80% identity with SEQ ID NO:1. The application clones a transcription factor RcETC3 from Rosa chinensis 'Yueyue Fen', and experiments show that the color of petals of the Rosa chinensis is obviously deepened in a transient gene silencing strain of the Rosa chinensis, indicating that the RcETC3 gene plays an important role in regulation of flower color of the Rosa chinensis, and provides an important basis for breeding of the Rosa chinensis.

The application belongs to the field of plant genetic engineering, and specifically discloses a Rosa chinensis RchChr3g0469531 (PIP2;1) gene and application thereof in regulation of thorn trait. The application finds that the RchChr3g0469531 (PIP2;1) located in the QTL1 interval is differentiated in the Rosa chinensis population with or without thorn and is specifically expressed in thorn tissue. Therefore, a modern Rosa chinensis Samantha (thorny Rosa chinensis variety R. 'Samantha') is taken as a material, a silencing vector is constructed through virus-mediated gene silencing (VIGS) to transform the Rosa chinensis, and the expression of the RcPIP2;1 gene in the Rosa chinensis is detected. The results show that the expression amount of the PIP2;1 is significantly reduced to about 25% after gene silencing, and combined with the thorn number phenotype, it is further determined that the RcPIP2;1 gene is a positive regulation factor of the thorn occurrence of the Rosa chinensis, and has important application value in cultivating new germplasm of few-thorn or thornless Rosa chinensis and improving field management of the Rosa chinensis.

The invention relates to the technical field of gene engineering, in particular to a rosa chinensis heat resistance regulation gene RhNCED2 and application thereof. The invention provides application of a product for promoting expression of an RhNCED2 gene or transcriptional coding protein thereof in enhancing heat resistance of Chinese rose, a nucleotide sequence of the RhNCED2 gene is shown as SEQ ID NO.2, and a nucleotide sequence of a specific fragment of the RhNCED2 gene is shown as SEQ ID NO.1. According to expression results of the RhNCED2 at different time points of high-temperature treatment of the Chinese rose, the RhNCED2 in a Chinese rose plant is instantaneously silenced, and the heat resistance of the Chinese rose is enhanced. Abnormal expression of the RhNCED2 can influence the heat resistance of the Chinese rose plants, silence of the RhNCED2 can weaken the heat resistance of the Chinese rose plants, and spraying of the abscisic acid can enhance the heat resistance of the Chinese rose plants.

The application relates to primers for synchronously detecting four kinds of viruses of Rosa chinensis and a method thereof, and belongs to the field of biology. The primer sequences are shown as SEQ ID. 1-8. Four main viruses (apple stem grooving virus, citrus tristeza virus, prunus necrotic ring spot virus and Rosa chinensis C virus) invading Rosa chinensis can be simultaneously detected by using the primers once. The method has the characteristics of high specificity, high detection efficiency, low cost, short time consumption and fg-level sensitivity.

The application discloses a Rosa chinensis disease prevention and growth promotion bacterium LX6 strain and application thereof, and belongs to the field of microorganism strains and application thereof. The Rosa chinensis disease prevention and growth promotion bacterium LX6 is preserved in the China General Microbiological Culture Collection Center on May 16, 2024, and has a preservation number of CGMCC No. 30657 and a classification name of Pseudomonas fluorescens. The bacterium strain has the ability to resist high-concentration salt ions and various heavy metal ions. The bacterium strain can inhibit the growth of a Rosa chinensis black spot pathogen, i.e., Discosia rosea, and can also antagonize the growth of plant pathogenic fungi, i.e., Colletotrichum gloeosporioides, Rhizoctonia solani, Fusarium oxysporum and Fusarium equiseti, so as to prevent and treat the Rosa chinensis black spot and powdery mildew, and promote the growth and flowering of the Rosa chinensis. The application provides a fine biocontrol strain for preventing and treating the diseases of the Rosa chinensis and promoting the growth of the Rosa chinensis, and has important significance for green prevention and control technology of the diseases of the Rosa chinensis and sustainable development of the Rosa chinensis industry.

The invention relates to a Chinese rose genetic transformation method, and belongs to the field of plant tissue culture. Specifically, the invention develops a genetic transformation method of the Chinese rose variety corola.

The invention relates to a rosa chinensis tissue culture seedling ex-vitro rooting special box and a matched rooting method thereof, the rooting special box comprises a transparent box body, a hole tray arranged at the bottom of the transparent box body, and a microporous breathable film covering the top of the transparent box body; the hole tray is formed by connecting a plurality of holes, the bottom of each hole is provided with a breathable drainage hole, and a gas exchange gap is reserved between the bottom of each hole and the transparent box body; the edge of the microporous breathable film is in sealed connection with the edge of the transparent box body, and a plurality of micron-sized small holes are uniformly formed in the microporous breathable film. The rooting method comprises the following steps: carrying out gradient seedling hardening on tissue culture seedlings; preparing and sterilizing a rooting substrate; performing cuttage operation; rooting and domesticating in the box; transplanting out of a box. According to the method, the special rooting box is used as a core carrier, through matrix formula optimization, accurate regulation and control of environmental parameters and operation process standardization, the rosa chinensis tissue culture seedling out-bottle efficient rooting which is independent of exogenous hormones and can synchronously achieve rooting and domestication is achieved, and the rooting rate and the seedling rate are both higher than those of a traditional in-bottle rooting technology.

The invention relates to the technical field of preparation and application of traditional Chinese medicine compositions, and particularly discloses a traditional Chinese medicine composition for treating Alzheimer's disease and a preparation method and application thereof. The traditional Chinese medicine composition is prepared from the following traditional Chinese medicine raw materials in parts by weight: 3-9 parts of ginseng, 3-10 parts of morinda officinalis, 6-12 parts of dogwood, 3-10 parts of rhizoma acori graminei, 3-10 parts of polygala tenuifolia, 3-6 parts of rhodiola rosea, 3-6 parts of China rose, 2-3 parts of hypericum perforatum, 3-6 parts of saussurea involucrate, 9-15 parts of cortex lycii radicis, 3-12 parts of golden cypress and 1-5 parts of agilawood. The traditional Chinese medicine composition provided by the invention accords with the traditional Chinese medicine treatment concept of tonifying deficiency and filling marrow, inducing resuscitation and soothing nerves, dredging collaterals and eliminating evil, and tonifying spleen and assisting transportation, not only can relieve damage of neurons, but also can reverse alzheimer disease related disorder signal channels in neuronal cells, microglial cells and astrocytes.

The utility model relates to the technology field of Chinese rose planting, and disclose a seedling raising device for Chinese rose planting, including fixed base, the top of fixed base is installed with two fixed frame, the inside of fixed frame is opened with adjusting groove, the inside fixed connection of adjusting groove has the fixed link, the inside of adjusting groove and the surface of fixed link all slidingly connect with adjusting block, the opposite surface of adjusting block all fixedly connect with the placing plate, the side away from the placing plate of adjusting block is opened with the slot of stirring, the top and the bottom of the inside of slot of stirring all are opened with the traction groove, the inside slidingly connects with the traction block of traction groove. The seedling raising device for Chinese rose planting, through can place the setting of the placing height of the placing plate conveniently adjust, can adjust the placing height of the placing plate to make different Chinese rose in different growth state all can have good growth space, and then make Chinese rose get sufficient sunlight, air and moisture, promote its healthy growth.

The invention discloses a pruning method for pruning red stumps of rosa chinensis, and belongs to the technical field of flower cultivation. According to the method, when the plant height of the cut flower branches of the cut flower roses reaches 30-40 cm and the cut flower branches are in the red strip stage, pruning and topping are conducted on the first branches with the compound leaf leaflet number larger than or equal to 7 from the plant top to the lower portion, the parts with the compound leaf leaflet number smaller than or equal to 5 of the top branches are cut off, the remaining branches after red stump cutting are reserved as stumps, nutrition is provided for the new flower branches through photosynthesis, and therefore the yield of the cut flower roses is increased. The flowering branches grow and develop better, and the problem that high-quality cut flowers cannot be produced due to insufficient nutrition of the cut flowering branches in a traditional pruning mode is solved. Meanwhile, a plurality of axillary buds can germinate from the cut flowering branch after the red stump is cut, the uppermost two axillary buds are reserved for development, and two flowers can be cultivated on one cut flowering branch, so that the yield of the cut-flower Chinese rose is increased. In addition, red stumps of cut-flower Chinese rose plants with inconsistent uniformity can be cut at the same time, and subsequently germinated buds are very tidy, so that farming management is facilitated, and flowers can be tidily grown.

The present application belongs to the technical field of flower cultivation, and particularly relates to an intelligent irrigation method for a multi-element cultivation mode of flowers under trees. The intelligent irrigation method for the multi-element cultivation mode of flowers under trees comprises the following steps: root irrigation of a growth promoter once a day during a flower bud stage, and keeping the soil moist during the rest period, wherein the growth promoter is formed by mixing a flutriarol activated ester solution and a hydroxyalkyladenine solution. In the present application, the growth promoter formed by mixing flutriarol activated ester and hydroxyalkyladenine is used for irrigation during the multi-element cultivation of flowers under trees. It is found through experiments that the growth promoter of the present application can promote the elongation of the stems of Chinese rose flowers and the increase in the diameter of Chinese rose flower buds at a low concentration, and compared with a single component, the growth promoter can reduce the dosage of the growth promoter, and is helpful to improve the quality and the grade of Chinese rose cut flowers and increase economic benefits.

The invention provides a composition for solving the blind branch phenomenon of cut-flower roses as well as a preparation method and application of the composition, and belongs to the technical field of fresh cut flower cultivation. Comprising the following raw materials: gibberellin, cytokinin, diethyl aminoethyl hexanoate, an amino acid water-soluble fertilizer and monopotassium phosphate, the raw materials are compounded together for application, and compound leaves on axillary bud parts of Chinese roses are removed, so that the cut-flower Chinese roses sprout quickly, the phenomenon of blind branches of the cut-flower Chinese roses is reduced, and normal growth and development of the soilless culture cut-flower Chinese roses are ensured. The method is simple to operate, quick in effect, low in cost, environment-friendly and safe, and the industrial problem of the blind branch phenomenon of the cut rosa chinensis is effectively solved.

The invention relates to the technical field of breeding of disease-resistant varieties of plants, in particular to application of miRNA (micro Ribonucleic Acid) of Chinese roses in gray mold resistance, the miRNA is novel-miR103, a mature sequence of the miRNA is shown as SEQ ID NO.1, and a precursor sequence of the miRNA is shown as SEQ ID NO.2; an interference vector containing SEQ ID NO.3 is constructed through a short tandem target simulation method and transferred into Chinese roses, the gray mold resistance of the Chinese roses can be remarkably improved by down-regulating the expression of the miRNA, the resistance can be reduced by up-regulating the expression of the overexpression vector constructed through an artificial microRNA method and transferred into the Chinese roses, and the invention further discloses application of the miRNA interference preparation and application of the miRNA interference preparation in gray mold resistance breeding of the Chinese roses. According to the application of the Chinese rose miRNA in gray mold resistance, the problems of cost, pollution and pathogenic bacterium drug resistance of chemical prevention and treatment can be solved, a clear molecular target and a technical path are provided, an important foundation is laid for improvement of Chinese rose disease resistance breeding, and the application prospect in the aspect of Chinese rose gray mold prevention and treatment is good.

The application discloses a Rosa chinensis organ development regulation gene, a small peptide thereof and application, and belongs to the technical field of plant genetic engineering.The application provides a kind of gene RwCLV3 for regulating the development of Rosa chinensis flower organ, and the nucleotide sequence is shown as SEQ ID NO.1;The application effectively regulates the development of Rosa chinensis and Arabidopsis thaliana flower organ by means of genetic engineering, can significantly change the size of apical meristem and the development and quantity of flower organ.The application provides a new gene resource for improving plant flower organ development and improving ornamental.