Special nutrient solution for Chinese rose, its preparation method and application method

Abstract

The present invention discloses a special nutrient solution for Chinese rose, its preparation method and application method, belonging to the technical field of garden planting. Calculated by weight parts, the special nutrient solution for Chinese rose includes 30-150 parts of angelica polysaccharide powder, 5-10 parts of diammonia enzyme, 5-10 parts of methyl dihydrojasmonate, 100-300 parts of organic carbon solution and 500-800 parts of water. The present invention promotes the accumulation of anthocyanins in Chinese rose through various ways, maintains the red flower color of Chinese rose, and achieves the technical effect of improving the ornamental value of Chinese rose.

Description

Technical Field

[0001] The invention relates to the technical field of garden planting, and in particular to a special nutrient solution for roses and a preparation method and an application method thereof. Background Art

[0002] Rose is one of the top ten famous flowers in China and is widely planted in China. Flower color is one of the important ornamental traits of roses, and studies have found that anthocyanins have a very important effect on red flower color. Especially in the case of high temperature or shade in summer, environmental factors will inhibit the expression of anthocyanin synthesis genes and regulatory genes, reducing the production of anthocyanins. At the same time, high temperature increases the activity of polyphenol oxidase in the petals, accelerating the degradation of anthocyanins, thereby reducing the anthocyanin content in the petals, making the flower color lighter and reducing the ornamental value of roses. Summary of the invention

[0003] The main purpose of the invention is to provide a special nutrient solution for roses and a preparation method and an application method thereof, aiming to solve the problem that the ornamental value of roses is reduced under the influence of environmental factors.

[0004] To achieve the above object, the present invention provides a special nutrient solution for roses, which comprises, by weight, 30-150 parts of angelica polysaccharide powder, 5-10 parts of diaminopeptide, 5-10 parts of methyl dihydrojasmonate, 100-300 parts of organic carbon liquid and 500-800 parts of water.

[0005] Optionally, the diamine enzyme is a mixture of 5-aminolevulinic acid and polyamino acids.

[0006] Optionally, the ratio of 5-aminolevulinic acid to polyamino acid in the mixture is 3-4:1.

[0007] Optionally, the polyamino acid is at least one of polyglutamic acid, polyaspartic acid, polyornithine, and polylysine.

[0008] Optionally, the content of water-soluble small molecule substances in the organic carbon liquid is 100-200 g / L.

[0009] Optionally, the content of angelica polysaccharide in the angelica polysaccharide powder is 20-40%.

[0010] In addition, to achieve the above-mentioned purpose, the present invention also provides a method for preparing a special nutrient solution for roses, which is used to prepare the special nutrient solution for roses as described above, and the preparation method comprises the following steps:

[0011] Weigh 30-150 parts of angelica polysaccharide powder, 5-10 parts of diaminopeptide, 5-10 parts of methyl dihydrojasmonate, 100-300 parts of organic carbon liquid and 500-800 parts of water;

[0012] Mix the organic carbon solution with half of the amount of water and stir to obtain the first mixed solution. The stirring time is 0.5 - 1 h, the rotation speed is 300 - 500 r / min, and the temperature is 30 - 50 °C.

[0013] Add angelica polysaccharide powder to the first mixed solution, mix and stir evenly to obtain the second mixed solution. The stirring time is 3 - 6 h, the rotation speed is 150 - 300 r / min, and the temperature is 50 - 60 °C.

[0014] Add diaminase, methyl dihydrojasmonate and the remaining amount of water to the second mixed solution in sequence, continue to mix and stir evenly to obtain the third mixed solution. The stirring time is 1 - 2 h, the rotation speed is 300 - 500 r / min, and the temperature is 30 - 50 °C.

[0015] Cool the third mixed solution to room temperature, and bag or bottle it to obtain the special nutrient solution for Chinese roses.

[0016] Optionally, the angelica polysaccharide powder is subjected to grinding treatment, and the grinding particle size is 0.05 - 0.15 mm.

[0017] In addition, to achieve the above object, the present invention also provides a method for applying the special nutrient solution for Chinese roses. The special nutrient solution for Chinese roses described above is used for application. The application method includes the following steps:

[0018] Dilute the nutrient solution 200 - 500 times with water and mix evenly.

[0019] Spray the diluted nutrient solution once every 7 - 10 days from the budding stage to the full - blooming stage of Chinese roses.

[0020] Optionally, if the Chinese rose is a four - season Chinese rose, the spraying frequency is once every 15 - 20 days.

[0021] For the special nutrient solution for Chinese roses provided by the present invention, the angelica polysaccharide therein generates anthocyanins on the basis of sugar metabolism. The unstable anthocyanins combine with glycosyl groups to form stable anthocyanins, that is, the accumulation of cellular sugars can promote the synthesis of anthocyanins; the diaminase therein is a highly efficient biological stimulant, which can promote the synthesis of anthocyanins; the methyl dihydrojasmonate therein can promote the degradation of JAZ (Jasmonate ZIM - domain) protein in the jasmonic acid signaling pathway, further activate the downstream signal cascade, and finally promote the accumulation of anthocyanins, thereby promoting the red flower color of Chinese roses. In addition, the organic carbon solution, angelica polysaccharide and diaminase added in the present invention can not only promote the accumulation of anthocyanins in Chinese roses, but also promote the growth of Chinese rose plants, make the Chinese rose plants strong, improve the adaptability to adverse environments, reduce the accumulation of oxidative substances in the plants, and reduce the degradation of anthocyanins in Chinese roses, thereby maintaining the red flower color of Chinese roses. Brief Description of the Drawings

[0022] Figure 1 It is a schematic flowchart of the preparation method of a special nutrient solution for Chinese rose in an embodiment of the present invention;

[0023] Figure 2 It is a schematic flowchart of the application method of a special nutrient solution for Chinese rose in an embodiment of the present invention.

[0024] The realization of the object, functional features and advantages of the present invention will be further described with reference to the embodiments and the accompanying drawings. Detailed Embodiments

[0025] It should be understood that the specific embodiments described herein are only used to explain the present invention and are not used to limit the present invention.

[0026] The contents of anthocyanins, flavonoids and soluble sugars are the most important factors affecting flower color, and the content of anthocyanins has a great impact on red Chinese roses. The existing improvement of Chinese rose quality mainly focuses on traits such as blooming throughout the year, flower fragrance, plant height, number of petals, disease resistance and cold resistance, and there is less research on flower color and its regulation. The existing research on Chinese rose flower color mainly focuses on flower color classification, types of flower pigments, gene regulation of flower pigments, extraction and application of flower pigments, etc., and there is a lack of research on external regulation of flower color.

[0027] The present invention provides a special nutrient solution for Chinese rose. Calculated by weight parts, the nutrient solution includes 30 - 150 parts of angelica polysaccharide powder, 5 - 10 parts of diammonium enzyme, 5 - 10 parts of methyl dihydrojasmonate, 100 - 300 parts of organic carbon solution, and 500 - 800 parts of water.

[0028] The main active ingredient in angelica polysaccharide powder is angelica polysaccharide, which can generate anthocyanins based on sugar metabolism. Unstable anthocyanins combine with glycosyl groups to form stable anthocyanins, that is, the accumulation of cellular sugars promotes the synthesis of anthocyanins. In nature, there are many types of polysaccharides. After studying the effects of different types of polysaccharides on the content of anthocyanins in Chinese roses, it can be found that angelica polysaccharide has the greatest promoting effect on the accumulation of anthocyanins in Chinese roses. The effects of polysaccharide types on the content of anthocyanins in Chinese roses are shown in Table 1 below.

[0029] Table 1

[0030]

[0031] The dual amino acid enzyme used in this embodiment refers to a substance containing two types of biostimulants, 5-aminolevulinic acid and polyamino acids. 5-aminolevulinic acid is the first key precursor for the biosynthesis of various pigments in organisms and is an important substance and promoter for the biosynthesis of anthocyanins. The addition of polyamino acids, on the one hand, can utilize the characteristics of its large molecules and many active groups to encapsulate 5-aminolevulinic acid, enabling 5-aminolevulinic acid to be released with the slow degradation of polyamino acids, reducing the natural degradation amount of 5-aminolevulinic acid, improving the utilization rate of 5-aminolevulinic acid. At the same time, various small molecule amino acids after the degradation of polyamino acids can continuously and comprehensively provide nutritional and physiological regulation functions for plants, improving the effect of the dual amino acid enzyme as a whole. The polyamino acids used in this embodiment can be at least one of polyglutamic acid, polyaspartic acid, polyornithine, and polylysine.

[0032] Different types of biostimulants have different effects on the content of anthocyanins in rose flowers. Among them, the dual amino acid enzyme is the most beneficial for the accumulation of anthocyanins. The effects of different types of biostimulants on the content of anthocyanins in rose flowers are shown in Table 2 below.

[0033] Table 2

[0034]

[0035] Jasmonic acid is an important plant hormone and plays an important role in many aspects such as plant growth, reproduction, and stress resistance. The JAZ protein, an inhibitor of the jasmonic acid pathway, is a key factor regulating the jasmonic acid hormone response. Methyl dihydrojasmonate can promote the degradation of the JAZ protein in the jasmonic acid signaling pathway, further activate the downstream signal cascade, and ultimately promote anthocyanin accumulation, thereby promoting the red color of rose flowers.

[0036] In addition, organic carbon solution, angelica polysaccharide, and dual amino acid enzyme can not only promote the accumulation of anthocyanins in rose flowers, but also promote the growth of rose plants, make the rose plants strong, improve the adaptability to adverse environments, reduce the accumulation of oxidants in the plants, and reduce the degradation of anthocyanins in roses, thereby maintaining the red color of rose flowers.

[0037] The embodiment of the present invention also provides a preparation method of a special nutrient solution for rose flowers, as Figure 1 shown, the preparation method includes the following steps:

[0038] Step S10, weigh 30 - 150 parts of angelica polysaccharide powder, 5 - 10 parts of dual amino acid enzyme, 5 - 10 parts of methyl dihydrojasmonate, 100 - 300 parts of organic carbon solution, and 500 - 800 parts of water.

[0039] Step S20: Mix the organic carbon solution with half of the amount of water and stir to obtain a first mixed solution. The stirring time is 0.5 - 1 h, the rotation speed is 300 - 500 r / min, and the temperature is 30 - 50 °C.

[0040] Step S30: Add angelica polysaccharide powder to the first mixed solution, mix and stir evenly to obtain a second mixed solution. The stirring time is 3 - 6 h, the rotation speed is 150 - 300 r / min, and the temperature is 50 - 60 °C.

[0041] Before adding the angelica polysaccharide powder, it can be ground first so that the particle size of the ground angelica polysaccharide powder is 0.05 - 0.15 mm.

[0042] Step S40: Add diamino enzyme, methyl dihydrojasmonate and the remaining amount of water to the second mixed solution in sequence, continue to mix and stir evenly to obtain a third mixed solution. The stirring time is 1 - 2 h, the rotation speed is 300 - 500 r / min, and the temperature is 30 - 50 °C.

[0043] Step S50: Cool the third mixed solution to room temperature, and bag or bottle it to obtain the special nutrient solution for Chinese roses.

[0044] The embodiment of the present invention also provides a method for applying the special nutrient solution for Chinese roses, as Figure 2 shown. This application method includes the following steps:

[0045] Step S60: Dilute the nutrient solution 200 - 500 times with water and mix evenly.

[0046] Step S70: Spray the diluted nutrient solution once every 7 - 10 days from the budding stage to the full - blooming stage of Chinese roses.

[0047] The application cycle of the nutrient solution can be adjusted according to the type of Chinese roses. For example, when the type of Chinese roses is Rosa chinensis Jacq. var. semperflorens, the spraying frequency is changed to once every 15 - 20 days.

[0048] Example 1

[0049] A special nutrient solution for Chinese roses, which is composed of the following components: 150 parts of angelica polysaccharide powder, 5 parts of diamino enzyme, 5 parts of methyl dihydrojasmonate, 300 parts of organic carbon solution, and 500 parts of water.

[0050] The diamino enzyme is a mixture of 5 - aminolevulinic acid: poly - amino acid = 4:1, and the poly - amino acid is poly - glutamic acid: poly - aspartic acid: poly - ornithine: poly - lysine = 1:1:1:1.

[0051] The content of water - soluble small - molecule substances in the organic carbon solution is 100 g / L.

[0052] Among them, the content of angelica polysaccharide in the angelica polysaccharide powder is 20%.

[0053] Preparation method of the nutrient solution: Mix the organic carbon solution with half of the amount of water and stir for 0.5 h at a rotation speed of 500 r / min and a temperature of 50 °C to obtain a first mixed solution;

[0054] Add angelica polysaccharide powder with a grinding particle size of 0.15 mm to the first mixed solution, mix and stir for 6 h at a rotation speed of 300 r / min and a temperature of 50 °C to obtain a second mixed solution;

[0055] Add diamino enzyme, methyl dihydrojasmonate and the remaining water to the second mixed solution in sequence, continue to mix and stir for 0.5 h at a rotation speed of 300 r / min and a temperature of 30 °C;

[0056] Finally, after the stirring of the third mixed solution is completed, bag or bottle the mixture cooled to room temperature to obtain the nutrient solution special for Chinese roses.

[0057] Application method of the nutrient solution: Dilute the above nutrient solution 500 times with water and mix evenly, and spray it once every 10 d from the budding stage to the full-bloom stage of Chinese roses.

[0058] Example 2

[0059] A nutrient solution special for Chinese roses, the nutrient solution is composed of the following components: 30 parts of angelica polysaccharide powder, 10 parts of diamino enzyme, 10 parts of methyl dihydrojasmonate, 100 parts of organic carbon solution, and 800 parts of water.

[0060] Among them, the diamino enzyme is a mixture of 5-aminolevulinic acid: polyglutamic acid = 3.5:1.

[0061] Among them, the content of water-soluble small molecule substances in the organic carbon solution is 200 g / L.

[0062] Among them, the content of angelica polysaccharide in the angelica polysaccharide powder is 40%.

[0063] Preparation method of the nutrient solution: Mix the organic carbon solution with half of the amount of water and stir for 1 h at a rotation speed of 500 r / min and a temperature of 30 °C to obtain a first mixed solution;

[0064] Add angelica polysaccharide powder with a grinding particle size of 0.05 mm to the first mixed solution, mix and stir for 3 h at a rotation speed of 150 r / min and a temperature of 60 °C to obtain a second mixed solution;

[0065] Add diamino enzyme, methyl dihydrojasmonate and the remaining water to the second mixed solution in sequence, continue to mix and stir for 1 h at a rotation speed of 300 r / min and a temperature of 50 °C;

[0066] Finally, after the stirring of the third mixed solution is completed, the mixture cooled to room temperature is bagged or bottled to obtain the special nutrient solution for Chinese roses.

[0067] Application method of the nutrient solution: The above nutrient solution is diluted 200 times with water and mixed evenly, and then sprayed once every 8 days from the budding stage to the full-bloom stage of Chinese roses.

[0068] Example 3

[0069] A special nutrient solution for Chinese roses, which is composed of the following components: 100 parts of angelica polysaccharide powder, 7 parts of double amino enzyme, 8 parts of methyl dihydrojasmonate, 200 parts of organic carbon solution, and 650 parts of water.

[0070] Among them, the double amino enzyme is a mixture of 5-aminolevulinic acid: polyglutamic acid = 3:1, and the polyamino acid is polyaspartic acid: polyornithine: polylysine = 1:1:1.

[0071] Among them, the content of water-soluble small molecule substances in the organic carbon solution is 150 g / L.

[0072] Among them, the content of angelica polysaccharide in the angelica polysaccharide powder is 30%.

[0073] Preparation method of the nutrient solution: Mix the organic carbon solution with half of the amount of water and stir for 1 h at a rotation speed of 300 r / min and a temperature of 50 °C to obtain the first mixed solution;

[0074] Add the angelica polysaccharide powder with a grinding particle size of 0.10 mm to the first mixed solution, mix and stir for 4.5 h at a rotation speed of 250 r / min and a temperature of 50 °C to obtain the second mixed solution;

[0075] Add the double amino enzyme, methyl dihydrojasmonate and the remaining water to the second mixed solution in sequence, continue to mix and stir for 0.5 h at a rotation speed of 300 r / min and a temperature of 30 °C;

[0076] Finally, after the stirring of the third mixed solution is completed, the mixture cooled to room temperature is bagged or bottled to obtain the special nutrient solution for Chinese roses.

[0077] Application method of the nutrient solution: The above nutrient solution is diluted 300 times with water and mixed evenly, and then sprayed once every 7 days from the budding stage to the full-bloom stage of Chinese roses.

[0078] Comparative Example 1

[0079] A special nutrient solution for Chinese roses, which is composed of the following components: 100 parts of angelica polysaccharide powder, 200 parts of organic carbon solution, and 665 parts of water.

[0080] Among them, the content of water-soluble small molecule substances in the organic carbon solution is 150 g / L.

[0081] Among them, the content of Angelica sinensis polysaccharide in the Angelica sinensis polysaccharide powder is 30%.

[0082] Preparation method of the nutrient solution: Refer to Example 3.

[0083] Application method of the nutrient solution: Dilute the above-mentioned nutrient solution 300 times with water, mix evenly, and spray once every 7 days from the budding stage to the full-bloom stage of the rose.

[0084] Comparative Example 2

[0085] A special nutrient solution for rose flowers, the nutrient solution is composed of the following components: 7 parts of diaminase, 200 parts of organic carbon solution, and 758 parts of water.

[0086] Among them, the diaminase is a mixture of 5-aminolevulinic acid: polyglutamic acid = 3:1, and the polyamino acid is polyaspartic acid: polyornithine: polylysine = 1:1:1.

[0087] Among them, the content of water-soluble small-molecule substances in the organic carbon solution is 150 g / L.

[0088] Preparation method of the nutrient solution: Refer to Example 3.

[0089] Application method of the nutrient solution: Dilute the above-mentioned nutrient solution 300 times with water, mix evenly, and spray once every 7 days from the budding stage to the full-bloom stage of the rose.

[0090] Comparative Example 3

[0091] A special nutrient solution for rose flowers, the nutrient solution is composed of the following components: 8 parts of methyl dihydrojasmonate, 200 parts of organic carbon solution, and 757 parts of water.

[0092] Among them, the content of water-soluble small-molecule substances in the organic carbon solution is 150 g / L.

[0093] Preparation method of the nutrient solution: Refer to Example 3.

[0094] Application method of the nutrient solution: Dilute the above-mentioned nutrient solution 300 times with water, mix evenly, and spray once every 7 days from the budding stage to the full-bloom stage of the rose.

[0095] The test results of each trait index of the rose flowers in Examples 1-3 and Comparative Examples 1-3 are shown in Table 3 below.

[0096] Table 3

[0097] Embodiment Number of flowers Flower diameter (cm) Soluble sugar (mg / g) Anthocyanin (U / g) Fresh water 3 3.83 4.86 111.21 Comparative example 1 3.2 4.21 6.31 192.28 Comparative example 2 3.6 4.32 4.78 184.87 Comparative example 3 3.8 4.28 5.32 178.36 Example 1 4.1 5.28 6.36 223.36 Example 2 4.6 5.63 6.18 242.42 Example 3 4.5 5.33 6.89 235.68

[0098] As can be seen from Table 3 above, the average number of rose flowers in the three examples increased by 46.67% and 24.53% respectively compared with the water treatment and the comparative example treatment. The flower diameters increased by 41.34% and 26.78% respectively, the soluble sugars increased by 33.26% and 18.40% respectively, and the anthocyanins increased by 110.25% and 28.59% respectively. The effect of enhancing the anthocyanin content in rose flowers is significant and the prospect is broad.

[0099] It should be noted that in this article, the term "including", "comprising" or any other variant thereof is intended to cover non-exclusive inclusion, so that a process, method, article or system including a series of elements not only includes those elements, but also includes other elements not explicitly listed, or also includes elements inherent in such a process, method, article or system. Without further limitation, an element defined by the statement "including one..." does not exclude the existence of another identical element in the process, method, article or system including that element.

[0100] The serial numbers of the above embodiments of the present invention are only for description and do not represent the advantages and disadvantages of the embodiments.

[0101] The above are only the preferred embodiments of the present invention, and do not limit the patent scope of the present invention accordingly. Any equivalent structure or equivalent process transformation made by using the content of the specification and drawings of the present invention, or directly or indirectly applied in other related technical fields, shall be equally included in the patent protection scope of the present invention.

Claims

1. A special nutrient solution for Chinese roses, characterized in that, By weight parts, the special nutrient solution for Chinese rose includes 30 - 150 parts of angelica polysaccharide powder, 5 - 10 parts of double - amino enzyme, 5 - 10 parts of methyl dihydrojasmonate, 100 - 300 parts of organic carbon solution and 500 - 800 parts of water; The double - amino enzyme is a mixture of 5 - aminolevulinic acid and poly - amino acid, and in the mixture, the ratio of 5 - aminolevulinic acid to poly - amino acid is 3 - 4:

1.

2. The special nutrient solution for Chinese rose as claimed in claim 1, wherein, The poly - amino acid is at least one of poly - glutamic acid, poly - aspartic acid, poly - ornithine and poly - lysine.

3. The special nutrient solution for Chinese rose as described in claim 1, characterized in that, The content of water - soluble small - molecule substances in the organic carbon solution is 100 - 200 g / L.

4. The special nutrient solution for Chinese rose as described in claim 1, characterized in that The content of angelica polysaccharide in the angelica polysaccharide powder is 20 - 40%.

5. A preparation method of a special nutrient solution for Chinese rose, characterized in that, For preparing the special nutrient solution for Chinese rose as described in any one of claims 1 - 4, the preparation method includes the following steps: Weigh 30 - 150 parts of angelica polysaccharide powder, 5 - 10 parts of double - amino enzyme, 5 - 10 parts of methyl dihydrojasmonate, 100 - 300 parts of organic carbon solution and 500 - 800 parts of water; Mix the organic carbon solution with half of the parts of water and stir to obtain a first mixed solution. The stirring time is 0.5 - 1 h, the rotation speed is 300 - 500 r / min, and the temperature is 30 - 50 °C; Add the angelica polysaccharide powder to the first mixed solution, mix and stir evenly to obtain a second mixed solution. The stirring time is 3 - 6 h, the rotation speed is 150 - 300 r / min, and the temperature is 50 - 60 °C; Sequentially add the double - amino enzyme, methyl dihydrojasmonate and the remaining parts of water to the second mixed solution, continue to mix and stir evenly to obtain a third mixed solution. The stirring time is 1 - 2 h, the rotation speed is 300 - 500 r / min, and the temperature is 30 - 50 °C; Cool the third mixed solution to room temperature, bag or bottle it to obtain the special nutrient solution for Chinese rose.

6. The preparation method of the special nutrient solution for Chinese rose as described in claim 5, characterized in that, The angelica polysaccharide powder is subjected to grinding treatment, and the grinding particle size is 0.05 - 0.15 mm.

7. A method for applying a special nutrient solution for Chinese rose, characterized in that, Using the special nutrient solution for Chinese rose as described in any one of claims 1 - 4 for application, the application method includes the following steps: Dilute the nutrient solution 200 - 500 times with water and mix evenly; Spray the diluted nutrient solution once every 7 - 10 days from the budding stage to the full - blooming stage of Chinese rose.

8. The application method of the special nutrient solution for Chinese rose as claimed in claim 7, wherein If the Chinese rose is a four - season Chinese rose, the spraying frequency is once every 15 - 20 days.

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