The invention discloses a method for seedling raising and cultivation of polygonatum kingianum on the basis of seed breeding. The method comprises the following steps that 1, berries of picked polygonatum kingianum are subjected to after-ripening for 20-25 days first, and pulp parts are removed to obtain seeds of polygonatum kingianum; 2, fertilization, ploughing, pest killing and sterilization are carried out on seedling raising land; 3, sowing is carried out; 4, soil is kept moist in a seedling period, and the shading rate is controlled to be 70-80%; 5, transplanting is carried out, whereinseedlings are transplanted in a planting area with the plant spacing of 25-35 cm and the row spacing of 30-45 cm, and the seedlings are watered once 3-5 days after transplanting; 6, management in a growing period is carried out, wherein the management includes water management, weeding, topdressing, control over the shading rate and top sealing to remove moss; 7, harvesting is carried out. The method achieves refined and standardized seedling raising of polygonatum kingianum, not only is the seedling raising time shortened, the survival rate of seedlings is increased, the quality of the seedlings is improved, but also the seedling rate is high, the incidence rate of diseases and pests is low, the growth speed is high, rhizomes of polygonatum kingianum after harvesting are thick and strong,the quality is good, and the yield is high.