190 results about "Armillariella mellea" patented technology

The invention relates to an imitative wild cultivation technique of gastrodia, comprising site selection, hollow opening, seed selection, fungus material spread, gastrodia seeds placing, three-grade armillaria mellea placing, humus soil covering and cultivation conducting. The technique results from field study and research, a large number of repeated experiments and explorations in the practice of cultivation by a plurality of years and can stabilize the yield of the gastrodia, particularly improve the quality of the gastrodia cultivation with good appearance and character, close to the wild state. In addition, taking full advantage of the superior natural environment, the technique reduces drop in production caused by bacteria invading, improves the capacity of the gastrodia to combat natural adversities, reduces cost by getting materials from local resources and makes up for the shortage of large field cultivation so as to resolve the land contention with the farmland, promoting the development of the special industry of underwood planting of the ecological agriculture.

The invention discloses a production and application technology of a symbiotic armillaria mellea artificial fungus stick for Gastrodia elata Bl., and belongs to the technical field of Chinese medicinal plant planting. The technology mainly comprises the following steps: (1) preparation of raw materials of an armillaria mellea artificial fungus stick, and mixing; (2) production of the armillaria mellea artificial fungus stick and sterilization; (3) inoculation of the sterilized armillaria mellea artificial fungus stick and cultivation; (4) field use of the cultivated armillaria mellea artificial fungus stick and Gastrodia elata Bl. planting. Compared with the conventional Gastrodia elata Bl. planting technology, the technology has the advantages that agricultural waste is adopted to produce the symbiotic armillaria mellea artificial fungus stick for Gastrodia elata Bl., industrialized production can be carried out, the operation step of culturing a fungus bed or fungus stick 6 to 8 months before Gastrodia elata Bl. planting in the conventional technology can be eliminated effectively, the traditional planting period is shortened, the inoculation rate and the Gastrodia elata Bl. yield after white-head Gastrodia elata Bl. permanent planting can be improved effectively, earlier-stage growth nutrition feeding can be guaranteed, the Gastrodia elata Bl. yield can be improved greatly, and the use of natural fungus materials can be replaced and greatly reduced, so as to reduce damage to natural woods of Gastrodia elata Bl. planting, and protect the ecological environment.

The invention relates to a method for producing rhizoma gastrodiae companion armillaria mellea through liquid submerged fermentation. The method is characterized in that a production technology of the rhizoma gastrodiae companion armillaria mellea through the liquid submerged fermentation comprises five steps as follows: (1) strain isolation of the armillaria mellea; (2) mother culture of the armillaria mellea; (3) preparation of solid seed bacteria; (4) liquid fermentation culture; and (5) cultivar inoculation and culture of the armillaria mellea. According to the method, various culture media of the armillaria mellea are provided, and problems that the armillaria mellea is rapid in degradation and slow in growth of strains, low in mycelium yield and varied in size and quality of the strains are solved; compared with traditional solid fermentation, the production technology has the advantages that the growth cycle of finished cultivar products of the armillaria mellea is greatly shortened, in addition, the rate of finished products is increased, and large-scale factory production of the high-quality rhizoma gastrodiae companion armillaria mellea is realized.

The invention discloses an artificial wild gastrodia elata cultivation technique. The technique includes the following steps: collecting local wild fresh armillaria mellea mycelia, preparing primary mother strains in a test tube, and switching secondary solid strains; selecting loose loam, sandy loam good in drainage performance or sandy soil, wherein pH value of the soil is 5-6; preparing firewood for preparing mushroom sticks and mushroom branches; preparing a filling material; culturing the mushroom sticks; cultivating gastrodia elata. A mushroom stick culturing method and a gastrodia elata cultivating method are more suitable for growing of gastrodia elata; especially, in the cultivating process of the gastrodia elata, sophora flavescens powder or tobacco stem powder is utilized, so that the mycelia can be prevented from being eaten by insects; penicillin liquid is sprayed, so that mushroom contaminating and rotting can be prevented; the artificial cultivation technique of the gastrodia elata is enabled to be more scientific and more reasonable, and yield of the gastrodia elata is stable; product detecting shows that gastrodin content is 0.38%, and product requirements are met completely.

The invention belongs to the technical field of strain cultivation and relates to a method for preparing armillaria mellea strains by using cut logs. The method comprises the steps: (A) blending materials: weighing culture materials according to a formula of a culture medium, mixing uniformly, and adding water until the water content is 55-70%, wherein the culture medium is prepared from the following ingredients in parts by weight: 8-12 parts of dry branch segments of weed-tree branches, 12-18 parts of wheat bran, 8-12 parts of maize flour, 2-4 parts of soybean meal, 1 part of white sugar, 1 part of gypsum powder and 1 part of potassium dihydrogen phosphate; (B) bagging the uniformly mixed culture materials, sterilizing and inoculating; (C) culturing: culturing the inoculated material bags at the temperature of 20-25 DEG C until the material bags are full of armillaria mellea hyphae. According to the method, the culture medium is appropriate in ingredient and ratio and balanced in nutrition; during the preparation of the armillaria mellea hyphae, the culture medium is strong in water retention and sufficient in nutrition, and the obtained armillaria mellea hyphae are thick and strong and are high in survival ratio and yield.

The invention discloses a bagged gastrodia elata armillaria mellea and a preparation method thereof. The gastrodia elata armillaria mellea is prepared by using a culture medium and a polypropylene or polyethylene fungus bag smaller than 0.04mm. Based on the traditional technique, the invention solves the problem that the culture medium formula is simple and the nutritive factors are collocated unevenly and by using the fungus bag instead of glass bottle, the invention solves the technical problems of the prior art that the glass bottle is heavy and unsafe and cannot perform industrialization production. Through the invention, the cultured armillaria mellea is whiter, stronger and neat, is convenient to use and is free from potential safety hazard. Through the invention, the fungus growing time is shortened and multiple beneficial effects, like ensuring stable yields despite drought or excessive rain, are realized.

The invention discloses a preparation method of a bottled armillaria mellea strain. The method comprises the following steps of: mixing: weighing culture materials according to a culture medium formula and mixing uniformly in dry state; B, bottling: filling the uniformly-mixed dry culture materials into strain bottles; C, basketing and filling water: putting the filled strain bottles into a disinfection transition basket, immersing the entire basket into a water pool, and filling water; D, pre-sterilizing: pre-sterilizing the water-filled strain bottles; E, discharging: discharging the entire basket of pre-sterilized strain bottles out of a sterilizing stove; F, sealing: plugging and sealing the strain bottles with common strain sealing articles such as bottle plugs, bottle caps, cotton plugs and kraft paper; G, secondary sterilizing: transporting the sealed strain bottles into the sterilization stove once again for normal-pressure or high-pressure sterilization; and H, cooling and inoculating, wherein the strain bottles are required to be kept in upright state in the entire production process. Due to the adoption of the method, the bottling speed during armillaria mellea strain production can be increased, the sealing articles are prevented from being polluted, strain microbial contamination is reduced, and factory production is facilitated.

The invention provides an armillaria mellea strain related to the high yield and stable yield of rhizoma gastrodiae and a preparation method of the strain. The taxonomy name of the strain is A.nabsnona T.J.Volk&Burds., the strain is a newly recorded species of armillaria fungi in China, and the preservation registration number is CGMCC No.11901. The method comprises the steps of strain selection, separation and purification, identification and cultivation. By means of the armillaria mellea strain related to the high yield and the stable yield of rhizoma gastrodiae and the preparation method of the strain, a risk of low yield of rhizoma gastrodiae or 'empty pond' phenomenon of no yield caused by the fact that selected stains are improper is reduced; the strain and gastrodia elata f.glauca have a better dependency, and the original quality of gastrodia elata f.glauca under the large-scale artificial cultivation condition in the region mainly producing gastrodia elata f.glauca can be guaranteed.

The present invention discloses an efficient culture medium for achieving efficient cultivation of armillaria mellea by improving extracellular enzyme activity thereof. The efficient culture medium comprises the following components in parts by weight: polycaprolactone microspheres, porous bioactive calcium phosphate microspheres, pine needle powder, edible fungus residue, cucurmosin, expanded rice bran husks, yeast cell walls, and yeast. According to the efficient culture medium provided by the invention, the pine needle power is subjected to hydrolysis performed by modified cellulases, and is used in combination with the edible fungus residue and the cucurmosin. The expanded rice bran husks are fermented to prepare the armillaria mellea culture medium, so that reasonable combination and abundant nutrition can improve the extracellular enzyme activity of the armillaria mellea, thereby improving cultivation efficiency of an extracellular enzyme. The used modified cellulases exist in the culture medium for a long time so as to decompose cellulose continuously and collaboratively and provide nutrition for the armillaria mellea. The porous bioactive calcium phosphate microspheres are used to adsorb the loaded yeast for fermentation, so that activity of the yeast is increased and decomposition of the culture medium raw material is promoted, thereby facilitating absorption and application, and providing a foundation for efficient cultivation of the armillaria mellea.

The invention relates to a cultivation technique for gastrodia elata, in particular to a three-dimensional cultivation technique for the gastrodia elata. The three-dimensional cultivation technique for the gastrodia elata includes the following steps: A: material preparation: timber with the diameter of 5-10 cm is cut into small wood sections with the length of 80-120 cm, the small wood sections are dehydrated by 70%-80% through air-curing, cuts are formed in each wood section at the interval of 5-10 cm, and each wood section is provided with 2-4 rows of the cuts; B: bacterium culturing: matured armillariella mellea is cut into small sections with the length of 3-5 cm in the late autumn in the same year, the small sections are embedded into the cuts of the small sections, then, the wood sections are placed, in the process of placing, the wood sections with the armillariella mellea and the wood sections without the armillariella mellea are alternately placed, the wood sections are closely adjoined and covered with sand, soil is turned in 3-6mouths for one time, the wood sections are watered during the period and kept damp till hairy stuff like beard appears in the cutters in the wood sections, and then the bacterium culturing is completed; C: cultivation: seeding and plantation are conducted in the early spring in the next year, the wood sections with the armillariella mellea are first placed at the interval of 5-10 cm, one wood section without the armillariella mellea is placed between every 2-3 wood sections with the armillariella mellea, one layer is formed by placing every 7-10 wood sections, gastrodia elata seeds are placed around the armillariella mellea and covered by the soil in time after a first layer is formed, a second layer is formed, the operation of forming the first layer is repeated till five to six layers with the height of 40-70 cm are formed, and the soil with the height of 5-15 cm is covered on the layers. According to the three-dimensional cultivation technique for the gastrodia elata, the method of three-dimensional cultivation is adopted, operation is not difficult, economic benefit is high, a large quantity of filed is saved, and management is easy.

The invention relates to the technical field of edible fungus cultivation, in particular to a culture medium for armillaria mellea spore separation and culture, a method and application. The culture medium comprises a basic culture medium, wherein the basic culture medium is prepared from 150-220 parts of potatoes, 15-25 parts of sucrose, 15-25 parts of agar, 1000 parts of water and 150-200 parts of a solid matrix. The solid matrix is prepared from cereals or beans or grains or their combination. The culture medium is an oblique-plane culture medium. The method adopting the culture medium to perform armillaria mellea spore separation and culture comprises the steps that first, the basic culture medium is made; the solid matrix is soaked in water and then is put into the basic culture medium; after disinfection, sterilization and cooling are performed, armillaria mellea spores are introduced to be cultured into strains. The culture medium is suitable for shoestring and tissue block separation and spore separation on the armillaria mellea matrix, enables spores to be bred into stock cultures at a time, the time from separation to purification is 25 to 30 days, no tube transfer is needed in the midway, the strains can serve as test tube mother strains and can also directly serve as the stock cultures for usage, and the separation time and a breeding cycle of the armillaria mellea strains are shortened.

The invention relates to a nutrient solution capable of improving seed quality of rhizoma Gastrodiae. Each 1000 mL of the nutrient solution contains 10 to 15 g of sugar, 5.0 to 5.5 mg of ferrous sulfate heptahydrate, 11 to 13 mg of boric acid, 90 to 100 mg of ammonium chloride, 7.5 to 8.2 mg of copper sulphate, 22 to 25 mg of manganese sulfate, 12 to 15 mg of zinc sulfate and 30 to 50 ml of armillaria mellea fermentation broth. The nutrient solution can substantially improve the fruit setting rate and quality of seeds of rhizoma Gastrodiae, provides an excellent seed source for stable high yielding of sexual reproduction and improves economic benefits of production of rhizoma Gastrodiae.

The invention provides a method for culturing of Armillaria mellea and rapid high-yield cultivation of Gastrodia elata by using wood sheets. The method comprises the following steps: selection of a mother Armillaria mellea strain, culturing of an Armillaria mellea stock, fabrication and culturing of a three-grade Armillaria mellea cultivar, preparation of new firewood and a cellar bed, and placement of the new firewood and sowing. According to the invention, the Armillaria mellea cultivar is cultured through the following steps: sawing a weed tree applicable to growth of Armillaria mellea into unpeeled wood sheets with a length of 9 to 12 cm and a width of 2 to 4 cm; by weight, preparing weed tree sawdust, wheat bran, white sugar and gypsum into a culture medium, placing the obtained culture medium powder onto the bottom part of a wood sheet bag, then vertically placing the wood sheets of the weed tree into the wood sheet bag, placing the culture medium powder onto the bag, tying the bag mouth, placing the wood sheet bag into a sterilization tank, and carrying out continuous sterilization at a constant temperature of 100 DEG C for 20 hours or above; and after sterilization is completed, placing the Armillaria mellea stock into a culture chamber, and carrying out culturing at about 20 DEG C for 40 days so as to obtain the Armillaria mellea cultivar used for cultivation of Gastrodia elata or Polyporus umbellatus. The method provided by the invention replaces conventional mushroom-sticks for direct co-cultivation of Gastrodia elata or Polyporus umbellatus, achieves that cultivation and harvesting are in a same year, realizes one-time cultivation and harvesting for a plurality of times, has high and stable yield of Gastrodia elata, and fundamentally solves the phenomenon of empty cellar.

The invention discloses a method of cultivating Gastrodia elata and belongs to the technical field of planting of Gastrodia elata. The method comprises the steps of preparing Armillaria mellea, preparing mushroom sticks, inoculating, cultivating Gastrodia elata, and managing. By controlling the cultivation process of Armillaria mellea and modifying the method of cultivating Gastrodia elata, the yield of Gastrodia elata is increased; tubers of Gastrodia elata cultivated through the method are uniform in size, full and good in condition.

The invention provides a building method of an armillaria mellea effective liquid phase fingerprint and a standard fingerprint and relates to the quality management and quality control of an armillaria mellea fermentation product. The standard fingerprint of the armillaria mellea fermentation product is obtained by comparing an HPLC fingerprint of a deep level fermentation product of 10 batches of armillaria mellea liquid, carrying out processing on the obtained data and spectra by utilizing a fingerprint similarity commenting system of a Chinese medicine chromatogram. The method has the advantages of simple and convenient operation, stable and reliable result, high precision and good reproducibility, etc. The built standard fingerprint can effectively represent the quality of the armillaria mellea fermentation product and provides a novel and effective method for the quality control and true-false distinguishing of the armillaria mellea.

The invention discloses a feed for enhancing the immunity of lobsters. The feed is prepared from the following raw materials in parts by weight: 40-80 parts of corns, 15-25 parts of oat bran, 10-20 parts of radish leaves, 2-10 parts of fish oil, 2-8 parts of waxgourd peel, 5-15 parts of soybean protein, 1-8 parts of puffed meat and bone meal, 2-6 parts of potato protein powder, 2-6 parts of earthworm powder, 20-40 parts of sunflower seed meal, 2-8 parts of yeast protein powder, 1-4 parts of yellow mealworm, 1-6 parts of peanut meal, 5-10 parts of rice bran meal, 1-4 parts of modified montmorillonoid, 12 parts of corn oil, 1-2 parts of selenium-enriched yeast, 0.1-0.4 part of phytase, 0.01-0.06 part of Schizophyllum commune, 0.04-0.08 part of Armillaria mellea, 0.02-0.05 part of monascus, 0.04-0.08 part of lysine, 0.1-0.4 part of methionine and 1-4 parts of a Chinese herbal medicine additive.

The invention discloses an indoor stereoscopic planting method for gastrodia elata. The method includes selecting houses meeting requirements on ventilation and heat insulation, and erecting shelves in the houses; laying bamboo fences on the shelves and laying agricultural films on the bamboo fences; laying composts on the agricultural films, and placing a layer of tree leaves on each agricultural film; uniformly sprinkling germination fungi mixed with pollen and seeds on the tree leaves, placing tree rods on the tree leaves, reserving gaps among the tree rods, placing tree branches in the gaps, placing pure armillaria mellea strains on the tree branches, further uniformly sprinkling a layer of germination fungi mixed with pollen and seeds on the pure armillaria mellea strains on each agricultural film, and covering the agricultural films by composts and tree leaves. The height of each layer of each shelf is 40cm, and the width of each shelf ranges from 70cm to 80cm. The tree leaves are soaked by water before being placed on the agricultural films. The indoor stereoscopic planting method for the gastrodia elata has the advantages that resources, lands and labor can be saved, and difficult problems in various aspects such as replanting resistance and disease and pest injury prevention of common planting methods can be solved.

The invention discloses food-grade health tea soluble granules capable of improving sleep, and belongs to the field of nutritional foods. Effects are excellent, and efficacy is quick. The food-grade health tea soluble granules comprise raw materials such as poria cocos, wolfberry, spine date seed, and armillaria mellea; is capable of reinforcing the vital energy and consolidating the constitution, enhancing the immunity, and reinforcing Qi and nourishing blood, and has advantages of excellent effects and quick efficacy.

The invention discloses special fodder for marketing gooses every 56 days. The special fodder comprises, by weight, 20-30 parts of extruded corn, 10-15 parts of wheat, 20-30 parts of millet powder, 2-5 parts of lard oil, 1-3 parts of carrots, 0.5-1.5 parts of grape seed oil, 15-25 parts of fish meal, 1-3 parts of earthworm powder, 2-5 parts of soybean protein concentrate, 2-6 parts of chrysanthemum dregs, 10-25 parts of cooked soybean meal, 5-10 parts of fermented biological protein, 2-6 parts of enzymolysis feather powder, 2-8 parts of scallop powder, 10-15 parts of maize germ dregs, 1-3 parts of rock flour, 2-4 parts of se-enriched yeast, 1-5 parts of Chinese herbal medicine additives, 0.02-0.08 part of compound enzymes, 0.1-0.7 part of phytase, 0.1-0.8 part of white ginseng, 0.2-0.8 part of armillaria mellea, 0.05-0.09 part of rhizopus oryzae, 0.03-0.07 part of lysine and 0.04-0.1 part of methionine. The special fodder is green, healthy, balanced in nutrition, remarkable in weight put-on effect and low in cost.

The invention discloses a method for propagating Gastrodia elata Bl. by medicinal material residues. The method includes the steps of 1), filling containers with culture materials, wherein the containers are made of bamboo or/and wood; 2), burying the containers into a soil groove dug in advance, and uniformly spreading Armillaria mellea (Vahl) P. Kumm. on the culture materials of the containers, wherein the soil groove is 15-50 cm in depth, and the length and the width of the soil groove are determined according to the number of the containers; 3), uniformly putting germinating strains mixed with pollen of the Gastrodia elata Bl. in advance on the culture materials of the adjoining containers; 4), covering the containers with raw soil, and covering the surface of the raw soil with rotten bamboo leaves or tree leaves; 5), keeping the water content of the containers between 60% and 70%, controlling the temperature below 30 DEG C, keeping the soil moist and harvesting after a year. The method has the advantages of easiness in operation, low cost, capability of turning waste into wealth, high survival rate of the Gastrodia elata Bl., satisfactory yield and high quality, thereby having good popularization and application value.

The invention relates to a quality control method of an armillaria mellea concentrated liquid. The method includes character inspection, chemical component identification, measurement of density, a pH value, insoluble substances in water, and a microbial limit, and measurement of a peptide content, and adopts a higher concentration state for storage and transportation. By re-check and verification, the method can evaluate quality of the armillaria mellea concentrated liquid from a plurality of aspects.