Method for preparing armillaria mellea strains by using cut logs

A technology of armillaria and strains, applied in botany equipment and methods, applications, fertilizer mixtures, etc., can solve the problems of unbalanced nutrition in the culture medium, low survival rate of strains, poor water retention of segment wood, etc., and achieve water retention Strong, high survival rate, strong hyphae effect

Active Publication Date: 2014-04-02
CHONGQING FUXINYANG EDIBLE FUNGUS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, as far as the production technology of Armillaria miscella is concerned, the disclosed medium components and preparation methods cannot achieve ideal results in actual prod

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035]Cut 1-1.5cm diameter of fresh miscellaneous wood branches into 2-3cm long pieces 9 parts (by dry weight), 15 parts of bran, 10 parts of corn flour, 3 parts of soybean flour, 1 part of sugar, gypsum powder 1 part and 1 part of potassium dihydrogen phosphate, mix well, and control the water content to 60%. Put the mixed culture material into the strain bottle, and sterilize at 100°C for 10 hours. According to the standard requirements of NY / T528-2010 "Technical Regulations for the Production of Edible Fungus Strains", the operation management such as inoculation and cultivation is carried out, and the cultivation is carried out at 20-25°C. After about 45 days, the mycelia of Armillaria armillaria will cover the entire strain bottle. The obtained Armillaria strain has strong hyphae, dense and tough cords.

Embodiment 2

[0037] Cut 1-1.5cm diameter of fresh miscellaneous wood branches into 2-3cm long pieces 9 parts (by dry weight), 15 parts of bran, 10 parts of corn flour, 3 parts of soybean flour, 1 part of sugar, gypsum powder 1 part and 1 part of potassium dihydrogen phosphate, mix well, and control the water content to 60%. Put the mixed compost into the seed bottle and sterilize at 120°C for 1.5 hours. According to the standard requirements of NY / T528-2010 "Technical Regulations for the Production of Edible Fungus Strains", the operation management such as inoculation and cultivation is carried out. After culturing at 20-25°C, the Armillaria mycelium will cover the entire strain bottle in about 40 days. The obtained Armillaria strain has strong hyphae, dense and tough cords.

Embodiment 3

[0039] Cut 1-1.5cm diameter of fresh miscellaneous wood branches into 2-3cm long pieces 9 parts (by dry weight), 15 parts of bran, 10 parts of corn flour, 3 parts of soybean flour, 1 part of sugar, gypsum powder 1 part and 1 part of potassium dihydrogen phosphate, mix well, and control the water content to 60%. Put the mixed culture material into the strain bag, and sterilize at 100°C for 10 hours. According to the standard requirements of NY / T528-2010 "Technical Regulations for the Production of Edible Fungus Strains", the operation management such as inoculation and cultivation is carried out at 20-25°C, and on the 10th day, triacontanol is sprayed on the mouth of the bag with a concentration of 0.2 mg / m 2 , About 32 days Armillaria armillaria mycelium covers the whole strain bottle. The obtained Armillaria strain has strong hyphae, dense and tough cords.

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Abstract

The invention belongs to the technical field of strain cultivation and relates to a method for preparing armillaria mellea strains by using cut logs. The method comprises the steps: (A) blending materials: weighing culture materials according to a formula of a culture medium, mixing uniformly, and adding water until the water content is 55-70%, wherein the culture medium is prepared from the following ingredients in parts by weight: 8-12 parts of dry branch segments of weed-tree branches, 12-18 parts of wheat bran, 8-12 parts of maize flour, 2-4 parts of soybean meal, 1 part of white sugar, 1 part of gypsum powder and 1 part of potassium dihydrogen phosphate; (B) bagging the uniformly mixed culture materials, sterilizing and inoculating; (C) culturing: culturing the inoculated material bags at the temperature of 20-25 DEG C until the material bags are full of armillaria mellea hyphae. According to the method, the culture medium is appropriate in ingredient and ratio and balanced in nutrition; during the preparation of the armillaria mellea hyphae, the culture medium is strong in water retention and sufficient in nutrition, and the obtained armillaria mellea hyphae are thick and strong and are high in survival ratio and yield.

Description

technical field [0001] The invention belongs to the technical field of strain cultivation, and relates to a method for making armillaria strains by utilizing segment wood. Background technique [0002] Armillaria mellea (Armillaria mellea), also known as Armillaria mellea, honey-colored fungus, honey mushroom, belongs to the fungal phylum, basidiomycotina, layer fungi, non-compartment basidiomycete subclass, agaricales, Tricholomaceae, honey ring Fungus, is a kind of medicinal and edible fungus. The fruiting body is generally medium-sized; the diameter of the cap is 4-14cm, light khaki, honey-colored to light yellow-brown; when old, it is brown, with flat or erect small scales in the middle, sometimes nearly smooth, with stripes on the edge; the flesh is white; The gills are white or slightly fleshy pink, and dark brown spots often appear when old; the stipe is slender, cylindrical, slightly curved, the same color as the cap, fibrous, soft and hollow inside, and slightly en...

Claims

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Application Information

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IPC IPC(8): A01G1/04C05G3/00
Inventor 马明山岳忠涛
Owner CHONGQING FUXINYANG EDIBLE FUNGUS
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