215 results about "Armillaria" patented technology

The invention discloses a cultivation method for interplanting Polyporuses and Gastrodia elata BIs, which comprises the following technologies: cultivating halimasch, producing seeds of Gastrodia elata BIs, sowing Polyporuses seeds, choosing farmlands as ponds, seeding, and methods for managing and harvesting the Polyporuses and the Gastrodia elata BIs. The cultivation method disclosed by the invention has the benefits that farmlands for growing crops is not occupied, dry branches and fallen leaves among various shrub woods, idle lands under forests, halimasch resources and soil resources arefully used, the labor efforts are saved, and the ecological, economic and social benefits are significant.

The invention discloses an artificial cultivation method of grifola, and relates to artificial cultivation methods of the grifola. The artificial cultivation method of the grifola comprises the following steps: a step (1) is cultivation of honey fungus branches; a step (2) is planting of grifola seeds; a step (3) is management after the planting; and a step (4) is harvesting. According to the artificial cultivation method of the grifola, an advanced and appropriate honey fungus strain expanding propagation and fungus branch cultivation method is adopted, cultivated honey fungus branches are strong and combined with new tree sticks and can provide rich nutrients for the rapid survival and the growth of the grifola seeds, and therefore the survival rate and the unit output of the grifola seeds are improved. The artificial cultivation method of the grifola is simple, convenient and high in unit output and yield.

The invention relates to a method for producing rhizoma gastrodiae companion armillaria mellea through liquid submerged fermentation. The method is characterized in that a production technology of the rhizoma gastrodiae companion armillaria mellea through the liquid submerged fermentation comprises five steps as follows: (1) strain isolation of the armillaria mellea; (2) mother culture of the armillaria mellea; (3) preparation of solid seed bacteria; (4) liquid fermentation culture; and (5) cultivar inoculation and culture of the armillaria mellea. According to the method, various culture media of the armillaria mellea are provided, and problems that the armillaria mellea is rapid in degradation and slow in growth of strains, low in mycelium yield and varied in size and quality of the strains are solved; compared with traditional solid fermentation, the production technology has the advantages that the growth cycle of finished cultivar products of the armillaria mellea is greatly shortened, in addition, the rate of finished products is increased, and large-scale factory production of the high-quality rhizoma gastrodiae companion armillaria mellea is realized.

The invention discloses an artificial high yield and high quality polyporus umbellatus cultivation method. The method comprises the following steps that a slope and a flat ground, with the altitude ranging from 900m to 1200m, in a mountainous area are selected, ring-cupped oak mushroom sticks which are cultivated by high-quality oak honey mushrooms and are suitable for growing of polyporus umbellatus are selected one year before the polyporus umbellatus is planted; six mushroom sticks and healthy, strong, elastic and purely wild grey polyporus umbellatus and black polyporus umbellatus which are cultivated in advance and are free of plant diseases and insect pests are planted in each pit in November of the year or March of the next year; 20-30cm pits are dug, and the sticks are placed in a ridging mode and are planted into only one layer; when the polyporus umbellatus is planted in March in spring or early November in fall, mulching film covering is carried out. The artificial high yield and high quality polyporus umbellatus cultivation method has the advantages of reducing planting attitude of the polyporus umbellatus, improving planting environment, raising the ground temperature and prolonging growing time because mulching film covering is additionally carried out, facilitating mushroom collection in advance and satisfying aerobic polyporus umbellatus and honey mushrooms because the mushroom sticks are cultivated in advance and are planted in a one-layer mode.

A special nutritive liquid for increasing the yield of gastrodia tube features that on the basis of conventional nutritive liquid, the aspartic acid and glutamic acid, or gourmet powder are used in it for balancing between different nutrients and compensating the functions of shoestring fungus. Its advantage is simple preparing process.

The invention discloses a three-dimensional composite planting method for gastrodia elata and polyporus under a wood. The three-dimensional composite planting method comprises the following steps of selecting wild gastrodia elata as a seed, selecting wild polyporus as a seed, selecting armillaria mellea, selecting germination fungus, preparing the fungus, selecting a filed, preparing soil, digging a cellar, cultivating and fixing a fungus bed, cultivating polyporus, cultivating seeds of gastrodia elata, dressing and sowing seeds, transplanting, managing under a wood, and harvesting goods. By adopting the three-dimensional composite planting method, idle area under a magnolia officinalis wood and armillaria mellea can be fully utilized, in the same cellar, the polyporus is planted on a lower layer, and the gastrodia elata is planted on an upper layer. The polyporus and the gastrodia elata are interplanted to share the armillaria mellea to furthest utilize the armillaria mellea. Waste fungus and the armillaria mellea mycelia can be used for supplying nutrients to the wood after harvesting, and transportation of waste fungus can be avoided.

The present invention discloses a kind of Armillaria luteo-virens ZJUQH CGMCC No.1884. Armillaria luteo-virens is cultured in Ca's culture medium. It has flat pileus with inwards rolled edge, medium-sized fruiting body in 5-12 cm width, white or yellowish thick flesh, dense and wide fungus plaits, white or yellow cylindrical stipe of 2-8 cm length and 2-2.5 cm diameter, and annulus in the middle position. The present invention provides new way for developing new edible and medicinal fungus and metabolite.

The invention discloses an armillaria luteo-virens fibrinolytic enzyme and a production method thereof. Fermentation and cultivation are made to armillaria luteo-virens ZJUQH CGMCC No.1884 to get fermented liquid and the armillaria luteo-virens fibrinolytic enzyme is separated and purified from the fermented liquid. The invention first realizes that artificial cultivated armillaria luteo-virens ZJUQH CGMCC No.1884 can be used for producing the fibrinolytic enzyme. The produced armillaria luteo-virens fibrinolytic enzyme takes agricultural and sideline products such as bean pulp, soybean flour and wheat bran with low cost as the main raw materials, thereby being safe and nonpoisonous. Being produced with a liquid fermentation method, the armillaria luteo-virens fibrinolytic enzyme is a hyper strain with relatively higher yield among the reports of epiphytes. Extraction and purification can be carried out easily and the armillaria luteo-virens is harmless, frozen-dry health products that contain thalli and enzyme liquid can be directly developed, thus providing a new thought and an evidence for developing and using novel edible fungus used in foods and medicines.

The invention belongs to agriculture planting, and in particular relates to a gastrodia elata cultivation technology which specifically comprises the following steps of: selecting gastrodia elata, covering the gastrodia elata by fine soil with adequate water, and putting the gastrodia elata at a constant temperature of 18-28 DEG C to grow until flower; pollinating after the maturation of pollen; after the maturation of fruits, picking seeds, and mixing the seeds with germination bacteria; sawing broad leaf tree twigs into 30-40 centimeters of small segments, chipping 4-6 scaly small holes on the small segments, and putting armillaria mellea into the scaly small holes to be used as bacteria; selecting sandy loam field with gradient, digging ridges, uniformly spreading the seeds over ridge bottoms, laying the bacteria in the ridges which is spread with the seeds, and laying 2-3 centimeters of fin soil over the bacteria; covering with sandy soil, and ridging for ranks; digging out the seeds in October of the next year to obtain poacynum pictum bail; during beginning of the winter to the fresh green of the next year, seeding the poacynum pictum bail according to step 5; and after the winter begins, picking the gastrodia elata. Compared with the conventional method, the cultivation period of the method is obviously shortened, and sufficient labor is provided for farmland; the cultivation process is simplified, and the probability of various crop failures is reduced; and the added additive is contributed to increasing the seed germination, and furthermore, the yield is increased.

The invention discloses a cultivation method for sexual propagation of gastrodiaelata with gastrodiainterplanted and belongs to the technical field of Chinese herbal medicine plantation. The cultivation method includes the first step of preparation of raw materials, the second step of selection and processing of planting land, the third step of primary planting of pollen seeds, the fourth step of gastrodiainterplantation, the fifth step of final-period management and the sixth step of harvesting. According to the cultivation method, primary planting of the pollen seeds is adopted for sexual propagation of gastrodiaelata, then in the end of the year and the beginning of the next year, the gastrodia is planted with original fungus-growing materials as fixed fungus beds based on original planting pits, and then only a small part of small fungus-growing materials are needed. The resources like the fungus-growing materials for sexual propagation of the gastrodiaelata and honey fungus are used for planting the gastrodia, therefore, the production cost of the gastrodia is greatly lowered, labor and the fungus-growing material resources are saved, and economic benefits are improved remarkably.

The invention discloses a grifola high-yield cultivation technology, and belongs to the technical field of cultivation. A method comprises the following steps: selecting and judging strains, selecting grifola wild funguses or propagules within two generations, evaluating and cultivating associated funguses, selecting robust and vigorous associated fungus strains to be cultivated, separating and purifying honey fungus strains from sclerotia, then conducting cultivation, and cultivating associated fungus sticks. According to the cultivation method, the scientific mode that the honey fungus strains and grifola 3+2 are arranged in an inclined mode is adopted, namely, the configuration structure that the three layers of honey fungus sticks are added on the two layers of the grifola strains is adopted, and the fungus stick plane inclined bodies are configurated in a staggered mode between layers. Cultivation ponding can shorten the grifola cultivation period by more than 2 years, the three-year period from planting to harvesting is reached, the high-yield effect that the output ratio is improved to be 1:10-15 is achieved, the production technology is stable, the grifola quality is high and safe, and the technical problems that the grifola artificial cultivation period is long, the production is unstable and the high-yield production can not be achieved for a long time are solved.

The invention provides a preparation method of a yellow green armillaria antioxidant active component. The method comprises the steps of carrying out superfine grinding on yellow green armillaria sporocarp to obtain superfine powder; carrying out water extraction treatment on the superfine powder for a plurality of times to obtain liquid supernatant; carrying out alcohol precipitation treatment on the obtained liquid supernatant by absolute ethyl alcohol; concentrating the liquid supernatant treated by alcohol precipitation to obtain a small molecular extract; dissolving the small molecular extract by pure water, and then filtering by an organic filter membrane; carrying solid-phase extraction on the obtained sample solution by C18 or C8 preparative chromatography to obtain the yellow green armillaria antioxidant active component. The method is simple in technology, easy to implement, rapid, efficient, good in repeatability, stable and controllable; the obtained yellow green armillaria antioxidant active component can be applied to preparing antioxidant drugs or health food.

The invention discloses a culture method of halimasch liquid bacterial strains. The culture method comprises the steps of parent strain preparation, shake-flask culture and fermentation tank culture. The culture method has the advantages that the raw material cost is low, the obtaining is easy, in addition, seed bacteria are subjected to fragmentation treatment, bacterial balls can become small and have the same size, the quality of the seed bacteria is improved, the growth speed of the bacterial balls is accelerated, the fermentation growth of the halimasch deep-layer liquid is fast, and the yield is greatly improved. The still standing suspension culture is adopted in the culture method, bacterial strains absorb nutrition from the culture media and absorb oxygen from air, and mycelia downwards grow until thick and strong rhizomorphs are grown and are fully filled into the whole fermentation tank. Halimasch of the obtained rhizomorphs can reach 35 to 40g through being metered by a dry weight method. Compared with the solid fermentation or the ordinary liquid deep-layer fermentation, the culture method has the advantages that the operation is simple, the separation is convenient, the period is short, the rhizomorph yield is high, and the active polysaccharide yield is also high and stable.

The invention provides an armillaria mellea strain related to the high yield and stable yield of rhizoma gastrodiae and a preparation method of the strain. The taxonomy name of the strain is A.nabsnona T.J.Volk&Burds., the strain is a newly recorded species of armillaria fungi in China, and the preservation registration number is CGMCC No.11901. The method comprises the steps of strain selection, separation and purification, identification and cultivation. By means of the armillaria mellea strain related to the high yield and the stable yield of rhizoma gastrodiae and the preparation method of the strain, a risk of low yield of rhizoma gastrodiae or 'empty pond' phenomenon of no yield caused by the fact that selected stains are improper is reduced; the strain and gastrodia elata f.glauca have a better dependency, and the original quality of gastrodia elata f.glauca under the large-scale artificial cultivation condition in the region mainly producing gastrodia elata f.glauca can be guaranteed.

The present invention relates to biological cleaning and repairing method for paper cultural relics and paper cultural relics is cleaned and reinforced through biotechnical process. Biological surfactant and re-compounded enzyme of proteinase, lipase, amylase, deformed streptococcus and armillaria are used in cleaning, repairing and reinforcing paper cultural relics. They are used in eliminating harmful crystals, plaque, and oil stain, and armillaria metabolite is utilized in improving acidifying, crisp and cracking state of paper cultural relics. The present invention can raise the quality of paper cultural relics.

The invention relates to a method for manufacturing halimasch production seeds from corncob grains. The method comprises the following steps of (1) material proportioning: corncob grains, wheat bran, corn powder and gypsum are taken according to weight parts; (2) material stirring: all ingredients are mixed and uniformly stirred, then, water is added until the water content in culture materials reaches 60 percent to 80 percent, and the materials are uniformly stirred; (3) bag loading: the uniformly stirred culture materials are loaded into material bags made of polyethylene or polypropylene materials; (4) sterilization: the material bags are sterilized at high pressure or normal pressure; (5) inoculation: halimasch strain blocks are dug and taken from an original strain bottle and are transferred into the material bags; and (6) culture: the inoculated material bags are transferred into a lucifugal culture room to be cultured under the temperature condition being 22 to 25 DEG C until the halimasch rhizomorph grows all over the material bags. The method provided by the invention has the advantages that the production cost is reduced, the finished product rate of the product is improved, and the great consumption of wood materials is avoided, so forest and the ecological environment are protected. The halimasch production seeds obtained by adopting the method can be used for bacterium material culture in the gastrodia elata planting.

A culture medium for culturing the shoestring fungus of gastrodia tuber is prepared through mixing agar, potassium dihydrogen phosphate, magnesium sulfate and potato juice, and adding the juice of oak bark.

The invention discloses an Armillaria selection marker. Hygromycin is adopted as the Armillaria selection marker. Antibiotics capable of inhibiting Armillaria growth are screened out by experiments; the eukaryotic expression vector pH2GW7-35S-EGFP is selected for agrobacterium transformation, the hygromycin is taken as the selection marker, and an EGFP gene is transferred into Armillaria with an agrobacterium-mediated method; GFP fluorescence is seen under a fluorescence microscope, which proves expression of pH2GW7-35S-EGFP in Armillaria, and the Armillaria can serve as a host cell for transgenic operation; establishment of a transgenic method based on Armillaria lays foundation for further research of Armillaria gene expression, and a genetic engineering research method for deep researchof biosynthesis and regulation of active components in Armillaria, the molecular mechanism of exchange between Armillaria and gastrodia elata nutrients and the like is provided.

The invention belongs to fermentation engineering of foods and drinks, and relates to a production process for fermenting tea by a microculture method, in particular to halimasch fermented tea and a production process thereof. The preparation method comprises the following steps of: preparing a culture medium; culturing first-stage seeds; performing amplification culture on second-stage seeds; inoculating and fermenting; and drying. The invention has the advantages that: halimasch is utilized to ferment tea, and the obtained fermented tea has double physiological effects, namely the pharmacological activity of tall gastrodia tuber and drinkability of tea, is easy to make and absorb, can fully integrate active ingredients of fermentation products, ensures that the resources of the fermentation products are fully utilized, and has good auxiliary and health-care effects in the aspect of improving human immunity.

The invention discloses a method for cultivating a fruiting body of a honey fungus, comprising the steps of: providing a honey fungus strain; preparing a culture medium: with compositions in a formula, by weight ratio: 18 to 25 percent of cereal crop and 75 to 82 percent of water; cultivating the honey fungus in the seed culture medium, inoculating the acquired honey fungus seed onto the prepared culture medium, and transferring the seed to the conditions of temperature of between 17 and 19 DEG C, relative humidity of 90 percent, and 12h scattered light each day for cultivation when the seed is in dark culture at a temperature of between 15 and 30 DEG C for 25 to 35 days, and the fruiting body begins to take shape after 10 to 15 days, and is picked at maturity, and a fungus embryo of the picked honey fungus fruiting body can serve as a functional feedstuff additive after being dried, the fungus embryo is extracted with water, the extract serves as a raw material for production of drugs, and the extracted waste residues serve as feedstuff. The method has the advantages of short outbreak cycle of the fruiting body, high output, wholly comprehensive utilization of the fungus embryo and non waste.

The invention provides a tissue culture type gastrodia elata seed breeding technology. The tissue culture type gastrodia elata seed breeding technology is characterized by comprising the steps of preparing materials, preparing a liquid culture material, making a culture medium I and auxiliary culture materials II and III, performing sterilization, obtaining seed-dressing germination strains IV, inoculating armillaria strains, adding sterile sawdust II, spreading a layer of seed-dressing germination strains IV or evenly distributing 5-8 small disinfected and sterilized generation-0 gastrodia elata seeds, filling aseptic river sand III on a culture unit to make the single sealed culture unit V and then performing culture. The gastrodia elata seeds are bred in a tissue culture mode, all the raw materials are scientifically combined, the advantages of indoor stereo culture and breeding are fully utilized, and a modern scientific technique is applied to all links of seed breeding. The seeds cultured under the aseptic condition do not infect bacteria or infectious microbes. Due to the fact that breeding is performed indoors, damage caused by insect pests, rats and other animals can be avoided. The culture materials are full of nutriments, and cultured seeds are large and full. Due to high yield and high quality, high economic value is certainly produced, and a solid foundation is laid for further production.

The invention provides an imitation wild gastrodia elata cultivation method, and belongs to the field of Chinese herbal medicine planting. The method comprises the following steps that 1, a bacteria bed is arranged; 2, a fungus material is laid flat, and amillaria mellea cultispecies are inoculated; 3, endophytic fungus cultispecies and gastrodia elata capsules are mixed, and seed dressing endophytic fungi are obtained; 4, a layer of seed dressing endophytic fungi are laid flat on the fungus material, a layer of leaves are scattered, and earthing is conducted; 5, the bacteria bed is dug out, white hemp is fixedly planted at the two ends of the fungus material, a bacteria introduction material is placed, and earthing is conducted; 6, harvesting is conducted. Accordingly, when gastrodia elata is planted, sexual propagation and asexual reproduction can be conducted synchronously, the land utilization rate is increased, the cost is reduced, the yield per mu of dried arrow hemp products is187.2 kg each year, the output value is 42,000-66,000 yuan, compared with a traditional planting method, the yield per mu is 21,000-30,000 yuan higher, and the economic value is considerable.

A preparation method of armillaria concentrate comprises the following steps: inoculating a bacterial strain onto a sterile culture medium, and culturing the bacterial strain for 3-4 days at 27 degrees plus or minus 1 degree to obtain mycelium; in terms of parts by weight, taking 3-5 parts of glucose, 1-3 parts of peptone, 1-3 parts of yeast extract, 0.1-0.5 part of KH2PO4, 0.1-0.5 part of MgSO4, 0.001-0.003 part of NaCl and 0.05-0.1 part of vitamin B1, adding 100-150 parts of purified water, and uniformly mixing to obtain a culture solution; inoculating the mycelium into the culture solution, and fermenting for 3-5 days under the conditions that the temperature is 27 degrees plus or minus 1 degree, the rotating speed is 100-500 rpm, the ventilation amount is 100-150 L/h, and the pH value is 5.0; collecting fermentation liquid, filtering the fermentation liquid, and concentrating the fermentation liquid at reduced pressure till the density (at 20 DEG C) is 1.030-1.035 to obtain the armillaria concentrate. The preparation method of the armillaria concentrate has the advantages as follows: the composition of the culture medium is clarified, so that production operation is facilitated; the production cycle is shortened; the fermentation liquid is relatively high in yield; the adenosine content of the fermentation liquid is higher than that in the original production technology.

The invention discloses a method of cultivating Gastrodia elata and belongs to the technical field of planting of Gastrodia elata. The method comprises the steps of preparing Armillaria mellea, preparing mushroom sticks, inoculating, cultivating Gastrodia elata, and managing. By controlling the cultivation process of Armillaria mellea and modifying the method of cultivating Gastrodia elata, the yield of Gastrodia elata is increased; tubers of Gastrodia elata cultivated through the method are uniform in size, full and good in condition.

The invention provides a preparation method of an Armillaria luteo-virens antioxidant active component, which includes the following steps: superfine-grinding Armillaria luteo-virens fruiting body to obtain superfine powder; extracting with water for many times, collecting supernatant, carrying out alcohol precipitation on the supernatant with anhydrous ethanol, and concentrating the supernatant subjected to alcohol precipitation to obtain a low-molecular-weight extract; dissolving the low-molecular-weight extract with analytically pure water, filtering with an organic filter membrane, and performing solid phase extraction on the sample solution with C18 or C8 preparative chromatography to obtain a pretreated sample; separating and purifying the pretreated sample with C18 preparative chromatography, and collecting according to chromatogram peaks to obtain various test components; and carrying out antioxidant activity tests on the test components, and selecting the component with the best antioxidant activity as the Armillaria luteo-virens antioxidant active component. The method provided by the invention has the advantages of simple and rapid process, easiness in implementation, high efficiency, good repeatability and good stability and controllability; the obtained Armillaria luteo-virens antioxidant active component can be used for preparing antioxidant medicaments or health foods.

The invention relates to the technical field of Gastrodia elata cultivation, in particular to a method for cultivating Gastrodia elata by utilizing mulberry branches. The mulberry branches are bundled, and the corn flour is combined to prepare a paste and then soaked, so that the mulberry branches and the branches are immersed. The room is filled with a paste mixture, and Armillaria is inoculated on the mulberry stick to form a stick, and the stick and the seed dressing are rolled up with the paste mixture to form a roll, so that the protocorm formed by the Armillaria and the seed dressing can be Sufficient contact can improve the survival rate and the output of Gastrodia elata, and the paste mixture of the present invention can be easily decomposed and utilized, so as to provide nutrition for the growth process of Gastrodia elata. Better, it promotes the rapid growth of Gastrodia elata and improves the yield of Gastrodia elata.

The invention discloses a production method of a gastrodia elata associated honey fungus strain. The method comprises the following steps: selecting branches of a broad-leaved tree, cutting the branches into cylindrical short rods, and putting the short rods in a bottle; filling the bottle with a nutrient solution and covering the bottle with a bottle cap to conduct sterilization treatment; cooling the bottle to ordinary temperature, and then conducting inoculation; conducting cultivation at a certain temperature in a dark place. According to the method, the branches are taken as a culture medium, and the nutrient solution is added to provide sufficient nutrition for the honey fungus strain and shorten the production period of the strain. Meanwhile, by checking the germination, growth condition and contamination of mycelia at reasonable time, the strains that don't germinate and eat, grow bad and are contaminated are removed, so that the quality of the strain is effectively improved, the activity and momentum of the strain are ensured, and a high-quality strain is provided for gastrodia elata planting.