11 results about "Armillaria" patented technology

The invention relates to a medicine for repairing skin wounds and a preparation method thereof, and belongs to the technical field of biological medicines.The medicine is prepared from, by mass, 25-30 parts of bee embryo peptide, 8-10 parts of alkannin derivative, 10-15 parts of armillaria mellea polysaccharide, 8-10 parts of inonotus obliquus polysaccharide, 6-8 parts of notoginsenoside and 5-7 parts of astragaloside. The bee fetus peptide is a product which is more than 500Da and is obtained by performing step-by-step enzymolysis on bee larva freeze-dried powder through trypsin and bacillus subtilis neutral protease to obtain enzymatic hydrolysate which is less than 3kDa, performing purification through a G-50 sephadex column, performing ultrafiltration and performing freeze-drying. The alkannin derivative is obtained by reacting alkannin with succinic anhydride and glucosamine-polyethylene glycol. A special method is adopted for preparing the bee embryo peptide and alkannin derivatives, the bee embryo peptide and alkannin derivatives are matched with other components to play a synergistic role, and the effects of high safety, high skin cell proliferation promoting capacity, excellent antibacterial performance and the like are achieved.

This invention discloses a *Armillaria mellea* contact induction device and control system for improving the inoculation rate after *Gastrodia elata* seed germination. It relates to the field of co-cultivation technology of edible and medicinal fungi and medicinal plants, and includes a shell, a germination support module, a mycelial induction module, a contact bridge module, a nutrient pulse module, an environmental modulation module, a dual-evidence detection module, and a main control module. The main control module generates an inoculation window index and, after trial contact, switches between a maintained contact state and a withdrawal state based on the recovery curve to complete the contact induction and colonization maintenance of *Armillaria mellea* with *Gastrodia elata* protocorms.

The application discloses a kind of intensive planting methods of Gastrodia elata in factory, belong to Gastrodia elata planting technical field;Including the following steps:S1: stereoscopic planting partition, the water and fertilizer collection layer is the lowermost layer, above is the staggered area of Gastrodia elata planting and armillaria cultivation;S2: erect planting basket and armillaria cultivation package, Gastrodia elata planting layer sets planting basket, armillaria cultivation layer sets armillaria cultivation package;S3: fungus material cultivation, direct light is set around armillaria cultivation layer and is shielded;S4: Gastrodia elata sowing, planting basket is laid sawdust layer, sowing, and lays planting substrate;S5: temperature and humidity control, armillaria cultivation layer temperature 22~25℃, humidity 65%~70%;Gastrodia elata planting layer temperature 15~25℃, humidity 70%~90%, substrate humidity 50%~60%;Solve the armillaria fungus material needs with Gastrodia elata seed same pond or same basket planting, need to carry out fungus material transplanting, and cannot directly monitor intervention armillaria mycelium growth and the invasion fusion condition between Gastrodia elata seed, cannot directly intervene control and promote Gastrodia elata growth from growth source.

The application discloses an application of Armillaria mellea in degrading vomitoxin, and further discloses the Armillaria mellea Am-07-22 with a preservation number of CCTCC NO: M 20221044; the mycotoxin is vomitoxin or zearalenone. The strain Am-07-22 has the best degrading effect when the concentration of vomitoxin is 2 mu g / mL, and the degrading rate is 64.05%; the Armillaria mellea has the degrading effect on vomitoxin in two different fermentation raw materials, the degrading effect is good when adopting a solid-state fermentation mode for corn husks, and the degrading effect is good when adopting a liquid-state fermentation mode for corn yellow powder.

The present application relates to Armillaria cultivation technical field, especially to a kind of Armillaria wild-like cultivation method.The present application provides a kind of Armillaria wild-like cultivation method, comprising the following steps: (1) the shrub cotoneaster is soaked using nutrient solution;(2) the shrub cotoneaster after soaking is placed in planting pit, and Armillaria seed is scattered, and after covering soil, culture is carried out, and every 30~40d is sprayed once nutrient supplement;(3) after starting to mushroom every year, 2~3 times of nutrient enhancer are sprayed.The Armillaria wild-like cultivation method provided in the present application can improve the utilization efficiency of Armillaria on shrub cotoneaster by pretreating shrub cotoneaster, and nutrient supplement and nutrient enhancer are added during the growth of Armillaria and when mushrooming, which can significantly shorten the mushrooming time of Armillaria, and can prolong the duration of mushrooming and improve the total yield of Armillaria, improve the economic benefit of planting farmers.

The invention discloses a combined preparation for preventing and treating morchella diseases and application of the combined preparation, and belongs to the technical field of edible mushroom culture. The combined preparation comprises armillaria luteo-virens polysaccharide F3 and antibacterial peptide Thanatin, and sterile water is used as a carrier. Experimental results show that when the armillaria luteo-virens polysaccharide F3 and the antibacterial peptide Thanatin are combined for use, a remarkable synergistic inhibition effect is achieved on paecilomyces varioti causing white mold of morchella esculenta. In addition, the combined preparation has no obvious negative influence on the growth of mycelia of the morchella esculenta, and shows good safety. Therefore, the invention provides an efficient, safe and environment-friendly prevention and treatment strategy for the white mold of the morchella esculenta, which is beneficial to reducing the use of chemical pesticides, delaying the generation of drug resistance of pathogenic bacteria and promoting the healthy and sustainable development of the morchella esculenta industry.

The invention discloses a gastrodia elata factory intensive planting method, and belongs to the technical field of gastrodia elata planting. Comprising the following steps: S1, three-dimensional planting zoning: the lowest layer is a water and fertilizer collection layer, and the upper part is a gastrodia elata planting and armillaria mellea culture staggered area; s2, planting baskets and armillaria mellea culture bags are erected, the planting baskets are arranged on the gastrodia elata planting layer, and the armillaria mellea culture bags are arranged on the armillaria mellea culture layer; s3, fungus material culture, wherein direct light is arranged around an armillaria mellea culture layer for shielding; s4, sowing gastrodia elata, laying a sawdust layer on a planting basket, sowing, and laying a planting substrate; s5, temperature and humidity control: the temperature of an armillaria mellea culture layer is 22-25 DEG C, and the humidity is 65-70%; the temperature of the gastrodia elata planting layer is 15-25 DEG C, the humidity of the gastrodia elata planting layer is 70-90%, and the The problems that the armillaria mellea material needs to be planted in the same pond or the same basket with the gastrodia elata seeds, the material transplanting needs to be performed, the growth condition of the armillaria mellea hyphae and the invasion and fusion condition between the armillaria mellea hyphae and the gastrodia elata seeds cannot be directly monitored and intervened, and the growth of the gastrodia elata cannot be directly intervened, controlled and promoted from the growth source are solved.

The application discloses a kind of cultivation methods for solving ginseng continuous cropping obstacles, comprising the following steps: corn is sowed on Armillaria bed of ginseng planting ground.The application has the advantages that corn is sowed on Armillaria bed of ginseng planting ground, the problem of soil continuous cropping obstacles is solved, and ginseng can be cultivated for a long time.The ginseng obtained by cultivation contains ginsenoside ≥0.08mg / ml, ginsenoside base ≥0.15mg / ml, p-hydroxybenzyl alcohol ≥0.01mg / ml, balesensoside A ≥1.0mg / ml, balesensoside B ≥0.08mg / ml, and balesensoside C ≥0.02mg / ml.

The invention belongs to the technical field of microorganisms, and discloses a wood stick culture medium for inducing sclerotia of polyporus umbellatus, the wood stick culture medium contains hardwood small sticks, glucose, monopotassium phosphate, magnesium sulfate and water, and the mass ratio of the hardwood small sticks to the glucose to the monopotassium phosphate to the magnesium sulfate to the water is 2000: 2: 1: 1: 2000. The invention also discloses a method for inducing sclerotia of polyporus umbellatus. The method comprises the following steps: 1) preparing the stick culture medium according to claim 1; (2) disinfecting the polyporus umbellatus seed; and (3) inoculating an armillaria mellea strain, and carrying out co-culture on the armillaria mellea strain. According to the method, the growth cycle of the polyporus umbellatus sclerotia can be greatly shortened, and meanwhile the yield of the polyporus umbellatus sclerotia and the content of effective components can be increased.

The application discloses application of Armillaria mellea in degradation of zearalenone, adopts Armillaria mellea Am-07-22, and the preservation number is CCTCC NO: M 20221044; the degradation effect on zearalenone is good, the degradation rate can reach 79.37% when the concentration of zearalenone is 5 μg / mL; the optimal condition for degrading zearalenone is that the temperature is 27 DEG C, the culture time is 8d, the initial pH is 7.0, and the inoculation amount is 10%; and Cu 2+ The fermentation supernatant has the best promoting effect on zearalenone degradation.

This invention discloses a method for liquid culture of *Armillaria mellea* fruiting bodies, mycelial colonies, and mycelial cords. The culture method employed in this invention shortens the exposure time of the mycelial colony, reduces contamination by other microorganisms, increases aeration, and promotes the differentiation, formation, and development of *Armillaria mellea* fruiting body primordia. After the fruiting bodies mature, the three-component products of fruiting bodies, mycelial colonies, and mycelial cords can be harvested, with a total biological efficiency of up to 296%. This cultivation method achieves unparalleled biological efficiency compared to other methods, produces no waste, and yields higher production and economic benefits.