93 results about "Gastrodin" patented technology

The invention discloses a high-yield gastrodia elata planting method based on Tilia brachypoda fungus material optimization and seed ramie stress resistance activation. According to the high-yield gastrodia elata planting method based on Tilia brachypoda fungus material optimization and seed ramie stress resistance activation, yield increase and quality improvement are achieved through fungus material composite strengthening treatment and a composite strain synergistic inoculation technology. The method comprises the following steps: (1) fermenting a tilia brachypoda substrate by lactobacillus plantarum and soaking in a nano-composite nutrient solution to form a high-activity bacterial material; (2) nitrogen-fixing bacteria, disease-resistant actinomycetes and armillaria mellea are mixed and inoculated according to the proportion of 3: 1: 1, nitrogen supply is improved, and the occurrence rate of black rot is reduced; 3) activating a GASA stress-resistant gene of the seed hemp through a pulsed electric field (5 kV / cm, 10 microseconds), and enabling the germination rate to reach 88.5% in combination with a calcium signal channel strengthening technology; according to the method, the yield per mu of gastrodia elata is increased by 30%-40%, the gastrodin content is increased by 30% or above, the chemical pesticide dosage is reduced by 55%, the strain passage purity is stable, the method is suitable for large-scale planting in multiple climate areas, and an innovative solution is provided for green production of traditional Chinese medicinal materials.

The invention discloses fresh rhizoma gastrodiae preserved fruits and a preparation method thereof. The preparation method comprises the following steps: (1) pretreatment; (2) freeze thawing treatment; (3) steaming with honey; (4) performing ultrahigh-pressure honey processing and sugar permeation treatment; (5) coating treatment; (6) drying at variable temperature; and (7) packaging and sterilizing. According to the method disclosed by the invention, citric acid color protection is combined with coating liquid treatment, so that browning is effectively inhibited, and the color and luster preservation period is prolonged; a freeze-thawing combined ultrahigh pressure honey processing synergistic process is innovatively adopted, so that the sugar soaking time is greatly shortened, the production efficiency is improved, efficient and uniform sugar soaking can be realized, the sugar distribution is uniform, and the taste is better; the variable-temperature drying process reduces loss of heat-sensitive nutritional ingredients (such as gastrodin), the unique flavor of the fresh gastrodia elata is reserved to the maximum extent, and the honey gradually permeates and is fused with the flavor of the gastrodia elata to form a composite flavor, so that the product has both leisure flavor and health value.

The invention discloses a genetically engineered bacterium for biosynthesis of gastrodin as well as a construction method and application of the genetically engineered bacterium. According to the genetically engineered bacterium, through overexpression of sucrose permease CscB derived from escherichia coli W, sucrose phosphorylase Basp of bifidobacterium adolescentis, uridine triphosphoryl-glucose-1-phosphate uridine acyl transferase UgpA of bifidobacterium bifidum and UDP-glycosyl transferase RsUGT of hedyotis diffusa, an exogenous path for synthesizing UDP-glucose from sucrose is constructed in a cell, and the UDP-glucose is synthesized into UDP-glucose. And the glycosylation capability of the gastrodin precursor on hydroxybenzyl alcohol is enhanced. The invention also provides a construction method of the genetically engineered bacterium and a method for synthesizing gastrodin through whole-cell catalysis by using the strain and taking p-hydroxybenzyl alcohol and cane sugar as substrates. The genetically engineered bacterium is high in gastrodin synthesis efficiency, the molar conversion rate can reach 97.8%, the highest yield reaches 3.61 g / L, and a new green manufacturing scheme is provided for solving the problems that a traditional gastrodin production method is low in efficiency, high in cost, large in pollution and the like.

The present invention discloses Armillaria mellea, a fungal agent containing Armillaria mellea, and its application, and relates to the field of microorganisms. The present invention provides any of the following Armillaria mellea strains, all of which are deposited in the General Microbiology Center of the China Culture Collection Administration of Microorganisms: a) Armillaria mellea Armillaria sp. , the deposit number is CGMCC No.41696; b) Armillaria mellea Armillaria sp. , the deposit number is CGMCC No.41697; c) Armillaria mellea Armillaria sp. The three Armillaria mellea strains provided by the present invention can establish a good symbiotic relationship with Gastrodia elata. The use of the Armillaria mellea strains in accompanying sowing or planting of Gastrodia elata can improve the yield and quality of Gastrodia elata and increase the content of polysaccharides, gastrodin and p-hydroxybenzyl alcohol in Gastrodia elata.

In various embodiments, provided herein are host cells, methods, and pharmaceutical compositions comprising gastrodin, wherein the gastrodin is produced by a genetically modified plant or plant cell, fungal cell, yeast cell, insect cell, or bacterial cell. In certain embodiments, the present disclosure provides methods and compositions for the production of gastrodin. In yet other embodiments, the present disclosure provides enhanced cells and methods for producing gastrodin.

The invention discloses a headspace gas chromatography method for detecting ethanol residual quantity in gastrodin. The headspace gas chromatography method comprises the following steps: (1) preparing a test solution; preparing a reference solution; (3) performing chromatographic determination; and (4) calculating a result. According to the headspace gas chromatography method for detecting the residual amount of ethanol in gastrodin, the headspace equilibrium temperature and time are optimized, a chromatographic column with specific polarity and programmed heating conditions are adopted, the ethanol residue detection method special for a gastrodin matrix is established, matrix interference is effectively overcome, and the detection accuracy is high. The method has high sensitivity and high accuracy, realizes exclusive, accurate and reliable quantitative control of ethanol residues in gastrodin, and has strong practicability.

The present application provides gastrodia elata (Blume) alkaloids PFT201A, PFT201B and PFT203 and a preparation method thereof, and application thereof in the fields of cosmetics and medicine. Gastrodia elata Gastrodia elata (Blume) alkaloids PFT201A, PFT201B and PFT203 significantly promote the secretion of collagen type II in human dermal fibroblasts-adult (HDFa) at an extremely low concentration (0.1 μ M), and have no cytotoxicity at the concentration. These compounds can be used for preparing anti-aging drugs or skin care products.

The utility model relates to the technical field of dryers, and discloses a gastrodia elata medicinal material dryer with a temperature adjusting function, which comprises a support frame, a drying box and a heating box, a working motor is arranged at the lower position of the side edge of the drying box, and a fixing bolt for fixing is arranged between a mounting plate and a screen plate. According to the utility model, the temperature sensors are assembled on the two sides in the drying box, so that the temperature sensors can monitor the temperature in the drying box, and meanwhile, the heating condition of the heating resistor is adjusted by connecting a circuit at the position of the heating resistor and adopting a voltage adjusting mode under the arrangement of the heating resistor; the material inlet is obliquely formed in the side edge of the drying box, the stirring rod can be driven to rotate after the working motor works, and the stirring blades are fixed to the outer portion of the stirring rod, so that materials can be stirred under the matching of the stirring rod and the stirring blades, and the drying effect on gastrodia elata medicinal materials is improved.

The application relates to the field of biological medicine, and discloses a glucosyltransferase mutant, a coding gene of the glucosyltransferase mutant, a recombination carrier, a recombination strain, an enzyme preparation, and application of the glucosyltransferase mutant. The glucosyltransferase mutant is a point mutation at the R position of the amino acid sequence shown in SEQ ID NO:1, and 4-8 histidines are added at the N end. A method for preparing gastrodin comprises the following steps: contacting the glucosyltransferase mutant with p-hydroxybenzyl alcohol and uridine diphosphate glucose. The glucosyltransferase mutant provided by the application has higher specificity for the substrate p-hydroxybenzyl alcohol, thereby obviously improving the conversion rate of the conversion of p-hydroxybenzyl alcohol into gastrodin, and having a good industrial application prospect.

This invention belongs to the field of pharmaceutical synthesis technology, specifically relating to a synthetic process for tetraacetyl gastrodin. It involves first preparing tetraacetyl glucose bromide using phosphorus tribromide, then reacting the tetraacetyl glucose bromide with a sodium alkoxide to obtain a condensation product, which is then reduced to yield tetraacetyl gastrodin. Therefore, under anhydrous conditions, the brominated sugar does not undergo hydrolysis, avoiding the loss of the brominated sugar raw material and thus improving the yield.

This invention discloses a method for the efficient synthesis of gastrodin using recombinant Corynebacterium glutamicum, belonging to the fields of genetic engineering, fermentation engineering, and synthetic biology. This invention uses Corynebacterium glutamicum as the host bacterium and knocks out… pobA The gene was expressed, and the engineered strain WN01 / pB6 was constructed using the expression plasmid pEC-XK99E to express cladoid-pyruvate lyase, carboxylic acid reductase, and glycosyltransferase, achieving heterologous synthesis of gastrodin. Further overexpression was then performed. aroG Genes, overexpression of protein-coding genes that activate carboxylic acid reductase sfp The engineered strain WN05 / pB6 was able to synthesize 7.37 g / L of gastrodin at the shake flask level, and the gastrodin yield reached 17.77 g / L at 72 h of batch fermentation in a 5-L fermenter, with a yield of 0.247 g / L / h. This provides a brand-new biomanufacturing platform for the industrial production of gastrodin.

The present application relates to a kind of preparation method of barley and gastrodia elata beverage, belong to food processing technical field.The existing gastrodia elata beverage preparation method has the problems such as low active ingredient extraction efficiency, difficult to synergistically utilize barley functional components, flavor relies on exogenous addition, product is easy to precipitate etc..The present application is by the specific proportion of mixing maturation of barley and gastrodia elata, inoculation includes saccharomyces cerevisiae, lactobacillus plantarum and streptococcus thermophilus Compound starter carries out solid state fermentation, then it is by the compound enzyme preparation including alpha-amylase, beta-glucanase and pectinase enzymolysis and subsequent processing, and beverage is prepared.The method synergistically improves gastrodia elata element and barley beta-glucan enrichment, creates coordinated flavor, and improves product stability.The obtained beverage is mainly used to provide the flavor beverage with the nutritional and health care function of gastrodia elata and barley.

The application provides a method for establishing HPLC fingerprint of Changnyle capsule (I), which is characterized in that 16 active components including gastrodin, danshensu, quercetin-3-O-beta-D-glucose-7-O-beta-D-gentiopicroside, 3'-hydroxy puerarin, puerarin, 3'-methoxy puerarin, puerarin apioside, paeoniflorin, daidzin, rosmarinic acid, shikonin, isoquercitrin, ononin, daidzein, salvianolic acid B and calycosin are used as reference substances, HPLC is adopted, methanol-acetonitrile-0.5vol% phosphoric acid aqueous solution is used as a mobile phase, different batches of samples are detected, and finally the fingerprint of Changnyle capsule (I) is obtained. The fingerprint can comprehensively represent the main components in Changnyle capsule (I), thereby being beneficial to the quality control of Changnyle capsule (I).

The present application relates to the technical field of medicine, and discloses application of gastrodin in preparation of medicine for preventing and treating radioactive liver injury, wherein the radioactive liver injury is liver injury caused by ionizing radiation exposure. It is found that the gastrodin can effectively intervene in specific mechanism of the radioactive liver injury, significantly reduce the mortality of the radioactive liver injury mice, reverse the hepatic veno-occlusive disease and the core pathological features of liver fibrosis of the radioactive liver injury, and more specifically treat the radioactive liver injury. On the other hand, the gastrodin can be used before radiation exposure to prevent serious radioactive liver injury after radiation exposure.

The invention relates to the technical field of processing and preparation of medicinal and edible drinks, in particular to gastrodia elata Pu'er tea and a preparation method thereof. The preparation method sequentially comprises the following steps: treating crushed gastrodia elata decoction pieces with cellulase, and filtering to obtain first supernate and first decoction dregs; performing water extraction and filtration on the first medicine residue to obtain a second supernatant and a second medicine residue, and performing ethanol hot-dipping extraction on the second medicine residue twice to obtain an extracting solution; combining the extracting solution and the two supernatants, concentrating, adding povidone K30, stirring, performing ultrafiltration, and concentrating the permeate to obtain a gastrodia elata extracting concentrated solution; drying, crushing and activating Pu'er ripe tea, soaking in hot water for three times, combining tea soup, and filtering to obtain a Pu'er tea extracting solution; and mixing the two extracting solutions, adding maltitol, uniformly mixing, and spray-drying to obtain the gastrodia elata Pu'er tea powder. According to the technical scheme, the technical problem that gastrodia elata Pu'er tea in the prior art is not ideal in taste and appearance can be solved, the eating convenience is improved, the product is rich in gastrodin and tea polyphenol, and the tea powder is suitable for long-term storage to guarantee quality and large-scale production to guarantee efficiency.

The invention belongs to the technical field of microbial fermentation, and particularly relates to a plant lactobacillus YSH08 and application thereof in preparation of gastrodia elata leavening with the efficacy of reducing blood sugar and blood fat. The gastrodia elata fermentation product is prepared by inoculating a gastrodia elata fermentation agent and fermenting; wherein the leavening agent is prepared from a plant lactobacillus YSH08 or a kluyveromyces marxianus Hz09. The gastrodia elata leavening obtained by the method has the effects of reducing blood sugar and blood fat; in addition, an in-vitro digestion experiment shows that saponin, polyphenol, gastrodin, parishin A, parishin B and parishin C in the fermented product all have very good biological accessibility.

The invention discloses kluyveromyces marxianus SWU-392 and application thereof, and belongs to the technical field of microorganisms. In order to solve the problems that gastrodia elata is limited to be foodstuff due to the fact that the gastrodia elata contains p-methylphenol, hexanal and other bad flavor substances, and flavor improvement and active ingredient retention are difficult to consider in the prior art, yeast with the preservation number of GDMCC No: 67309 is applied to gastrodia elata fermentation, and the gastrodia elata is fermented in a solid state at the constant temperature of 30 DEG C for 4 days. Poor substances such as p-methylphenol and aldehydes in gastrodia elata are effectively degraded, the content of flower and fruit fragrance type flavor substances such as esters is remarkably increased, and meanwhile the content of gastrodin and p-hydroxybenzyl alcohol is slightly increased. The process is mild, safe, free of chemical additives, high in strain adaptability and easy to industrially amplify, collaborative optimization of flavor and efficacy of the gastrodia elata is achieved, and a green and efficient technical scheme is provided for food application of the gastrodia elata.

The invention belongs to the technical field of hyperspectral intelligent detection, and discloses a hyperspectral monitoring and preparation method of eurotium cristatum fermented gastrodia elata, which comprises the following steps: adjusting the water content of dried gastrodia elata or cutting fresh gastrodia elata into square blocks, placing in a fermentation bottle, sterilizing, inoculating eurotium cristatum spore suspension, uniformly mixing, placing in a constant-temperature incubator, and culturing for 0-12 days; regularly sampling and collecting a hyperspectral image, screening characteristic wavelengths by using a CARS-MLR algorithm, establishing a PLS-DA model, and performing pixel-level prediction and visual mapping on indexes such as gastrodin and p-hydroxybenzyl alcohol; gC-MS (gas chromatography-mass spectrometry) volatile substance analysis and sensory evaluation verify that the horse urine smell is remarkably reduced after fermentation, and pleasant fragrances such as mushroom flower fragrance and mint fragrance are enhanced. According to the method, the content of active ingredients such as polysaccharides and polyphenols is remarkably increased, the flavor of the gastrodia elata is improved, online nondestructive testing and intelligent control in the gastrodia elata fermentation process are achieved, and the fermented gastrodia elata product with low peculiar smell, high activity and enhanced functions is obtained.

The invention discloses a traditional preparation process of gastrodia elata line incense, and belongs to the technical field of traditional processes, and the traditional preparation process comprises the following steps: S1, AI-driven flavoring research and development: digitizing dimensions such as aroma concentration and chemical properties, combining a convolutional neural network algorithm, improving the flavoring efficiency by 10 times, shortening the customization response time by 36%, and meanwhile, realizing automatic optimization of an essence formula; the research and development period is shortened by 60%; s2, low-temperature wall-breaking extraction of gastrodia elata: extracting gastrodin by adopting supercritical CO2 extraction (parameters: 35 DEG C and 15 MPa) to avoid high-temperature damage, inclusion of an extract and beta-cyclodextrin to form a heat-resistant microcapsule, and improving the combustion tolerance to 300 DEG C; s3, step-by-step fusion: extracting frankincense resin through absolute ethyl alcohol, mixing the gastrodia elata microcapsules, the frankincense resin and borneol together, and performing manual rolling. Rolling and pressing are still manually carried out, the core material is rolled and formed in cooperation with a mold, and the situation that the incense body is compact due to high pressure is avoided.

The present application relates to the field of biological medical technology, in particular to a traditional Chinese medicine composition as GSK-3beta inhibitor and preparation and application thereof. The traditional Chinese medicine composition as GSK-3beta inhibitor comprises gastrodin and ganoderma triterpene, and the mass ratio of gastrodin and ganoderma triterpene is (1-4):1. The traditional Chinese medicine composition provided by the present application can inhibit the overexpression of GSK-3beta in AD model, thereby inhibiting the overphosphorylation of Tau protein and playing a protective role on Alzheimer's disease. In addition, the traditional Chinese medicine composition provided by the present application contains gastrodin and ganoderma triterpene, which are homoeopathic traditional Chinese medicine efficacy components, greatly improving the safety of the drug, and providing a train of thought for the application of the traditional Chinese medicine composition as a functional food.

The invention provides a composition for relieving beef cattle transportation stress syndrome and application thereof, the composition is prepared by premixing dexmedetomidine hydrochloride, gastrodin and normal saline for injection according to the mass concentration ratio, the concentration of dexmedetomidine hydrochloride is 60-65 g / mL, and the concentration of gastrodin is 20-30 mg / mL. The composition is applied to beef cattle before transportation, the composition is intramuscularly injected, and the total injection volume is 10-20 mL. After the composition is injected before beef cattle transportation, immune dysfunction can be effectively improved, excessive HPA axis activation is inhibited, cortisol secretion is reduced, oxidative damage is relieved, and the stability of intestinal flora is maintained, so that inflammation is relieved, nervous excitability is reduced, calming and anxiety resisting effects are achieved, beef cattle transportation stress response is reduced, and the beef cattle transportation effect is improved. And the method is simple to operate and low in cost, and provides theoretical and practical support for preventing the beef cattle transportation stress syndrome.

The invention belongs to the technical field of functional foods, and particularly relates to a gastrodia elata-dogwood composite health-care beverage and a preparation method thereof. Specifically, the gastrodia elata extract and the dogwood extract are respectively extracted, then a sweetening agent (such as white granulated sugar) is added for compounding to finally obtain the gastrodia elata and dogwood composite health-care beverage, and the beverage is light pink, clear and transparent, free of sediment, good in storage stability, harmonious in flavor, appropriate in taste, free of abrupt external taste, and capable of being eaten by people. Meanwhile, the gastrodia elata beverage is rich in functional components such as gastrodin, gastrodia elata polysaccharide, loganin and the like, and has a good health-care value. Meanwhile, the gastrodia elata and dogwood composite health-care beverage is simple in preparation method, low in cost and easy for large-scale production, so that deep processing of agricultural products such as gastrodia elata and dogwood is facilitated, and the gastrodia elata and dogwood composite health-care beverage has good practical application value.

1. Name of the design product: medicine packaging box (gastrodin tablet). 2. Use of the design product: the medicine packaging box is used for packaging (gastrodin tablet). 3. Design points of the design product: combination of shape and pattern. 4. Picture or photo best indicating the design points: front view.

The application provides a high-stability gastrodin production process, which takes gastrodin tetraacetate as a starting material, and sequentially undergoes pentaacetylation reaction, centrifugation and drying to obtain a gastrodin pentaacetate intermediate; then gradient saponification refining is adopted, that is, preliminary hydrolysis is first carried out by using a low-concentration lye, and then deep saponification is carried out by using a high-concentration lye; after saponification, the product is subjected to centrifugation, drying and crushing to obtain a finished product; an ultrasonic-assisted crystallization step is added after saponification, and an inert gas atmosphere coating is carried out after crushing. The glycosidic bond breakage caused by strong alkali is effectively avoided through gradient saponification, and the key problems of low product purity and poor stability in the existing process are solved in combination with ultrasonic crystallization and inert gas protection. The obtained gastrodin product has the remarkable characteristics of high purity, few impurities and excellent stability, and has the following indexes: purity ≥ 99.1%, related substances ≤ 0.45%, and content decrease rate ≤ 0.8% after 18 months of accelerated test.

The invention discloses a pharmaceutical composition for promoting neuron neogenesis and a method for promoting neuron neogenesis by using a gastrodia elata extract or an adenosine analogue. The gastrodia elata extract comprises gastrodin, gastrodigenin, a parishins compound, N6-(4-hydroxybenzyl) adenosine (T1-11) and 4-hydroxybenzaldehyde (4-hydroxybenzaldehyde). The invention also discloses a method for promoting neuron neogenesis by using the gastrodia elata extract or the adenosine analogue. The gastrodia elata extract provided by the invention has aging-retarding activity on nerve cells and can induce mouse hippocampal neuronal neogenesis.

This invention belongs to the field of pharmaceutical formulation technology, specifically disclosing a gastrodin transdermal patch and its preparation method. The patch consists of a backing layer, a drug-containing matrix layer, and an anti-adhesive layer, using gastrodin as the active ingredient. It employs HP-β-CD inclusion technology, combined with an HPMC and PVP polymer backbone and an oleic acid-menthol composite transdermal enhancement system. The preparation utilizes solvent casting and low-temperature vacuum drying processes, which are mild and controllable. This invention effectively solves the problems of difficult transdermal absorption, easy recrystallization, and low bioavailability of gastrodin. The resulting patch has excellent adhesion, no skin irritation, high transdermal penetration, and good skin biocompatibility. It can efficiently cross the blood-brain barrier, regulate the neuroinflammatory microenvironment in the brain, significantly inhibit the colonization and progression of non-small cell lung cancer brain metastases, and strongly reverse tumor-related anxiety and depression-like mood disorders. It provides a novel drug delivery regimen with great clinical translational potential for the non-invasive synergistic treatment of brain tumors and their associated mood disorders.

The utility model relates to gastrodia elata medicinal material crushing technical field discloses gastrodia elata medicinal material crushing device convenient for feeding, including support base, both sides of support base all are fixed with support board, the top of inside support board is hinged and is installed with the crushing cylinder through the pivot, the inside installation of cylinder cover has the observation glass, the inside middle position of observation glass is swingly provided with the rotary rod, and the bottom of rotary rod installs the scraper, the top of rotary rod installs the rotation block. The utility model discloses the crushing cylinder uses the cylinder cover of upper side as the flip, and the crushing cylinder is opened conveniently, and the gastrodia elata is put into the inside crushing cylinder and is crushed, and the inside setting of cylinder cover has scraper, observation glass and rotary rod etc. component, then it is convenient to observe the gastrodia elata division crushing state in the process of gastrodia elata crushing, realizes this crushing device convenient for feeding and observes the crushing process, and the practicality is stronger.

This invention discloses a pulverizing and processing device for gastrodin extraction, relating to the field of gastrodin extraction technology. It includes a mechanism cylinder and a support frame fixedly connected to the bottom outer side of the mechanism cylinder. A conical cover is fixedly connected to the upper side of the mechanism cylinder, and a sealing cover is detachably connected to the upper side of the conical cover. A first pulverizing mechanism is provided inside the sealing cover, and a second pulverizing mechanism is provided inside the mechanism cylinder. The mechanism cylinder includes a motor, a transmission rod fixedly connected to the motor output end, and a lifting assembly installed at the bottom inner side of the mechanism cylinder. The lifting assembly drives the motor to move up and down. A placement plate is rotatably connected to the upper end of the transmission rod. This pulverizing and processing device for gastrodin extraction uses a cooler to cool the upper side of the placement plate and the inside of the grinding cylinder, ensuring that the gastrodin pieces remain at a low temperature during the pulverizing process, thus avoiding heat accumulation that could affect the pulverizing efficiency.

The application discloses a purified polysaccharide of Gastrodia elata and a preparation method and application thereof, and belongs to the technical field of extraction of active components of Gastrodia elata. The method comprises the following steps: (1) adding an alcohol solution to Gastrodia elata powder for reflux extraction, and collecting filter residues; (2) dissolving the filter residues in pure water for ultrasonic extraction to obtain a filtrate; (3) adding anhydrous ethanol to the filtrate for low-temperature alcohol precipitation to obtain crude polysaccharide of Gastrodia elata; (4) performing deproteinization treatment on the crude polysaccharide of Gastrodia elata; (5) performing purification by using a macroporous resin; (6) dialyzing the crude polysaccharide purification liquid in pure water; (7) performing purification by using a first chromatographic column, and the filler is cellulose-based anion exchange resin; (8) performing purification by using a second chromatographic column to obtain the purified polysaccharide of Gastrodia elata; and the filler is dextran gel. The crude polysaccharide of Gastrodia elata is purified by using the above five purification means, and a high-purity purified polysaccharide of Gastrodia elata can be quickly, stably and efficiently obtained.

The invention provides a pharmaceutical composition for reducing blood fat and blood pressure and a preparation method thereof, and belongs to the technical field of biological pharmacy, the pharmaceutical composition comprises durian honey seed extract, brassica campestris root-allium wilt composite extract, gastrodin, rhynchophylline, eucommia ulmoides glycoside and puerarin. The durian honey seed extract is prepared by performing enzymolysis on durian honey seeds through cellulase and pectinase, performing enzymolysis step by step through pepsin and ginger protease, adding a DES cosolvent, performing ultrasonic extraction, and performing MCI GEL CHP20P column purification. The red brassica campestris root-allium cervi composite extract is prepared by fermenting red brassica campestris roots and allium cervi herbs through lactobacillus rhamnosus and purifying fermentation liquor through a macroporous adsorption resin HP2MG column. All the components are matched for use, triple optimization of safety-drug effect-mechanism cooperation is achieved, the problems that western medicine is single in target spot and remarkable in side effect after being used for a long time are avoided, and the defects that traditional Chinese medicine is extensive in preparation and unstable in curative effect are overcome.