64 results about "Gastrodin" patented technology

The invention discloses a high-yield gastrodia elata planting method based on Tilia brachypoda fungus material optimization and seed ramie stress resistance activation. According to the high-yield gastrodia elata planting method based on Tilia brachypoda fungus material optimization and seed ramie stress resistance activation, yield increase and quality improvement are achieved through fungus material composite strengthening treatment and a composite strain synergistic inoculation technology. The method comprises the following steps: (1) fermenting a tilia brachypoda substrate by lactobacillus plantarum and soaking in a nano-composite nutrient solution to form a high-activity bacterial material; (2) nitrogen-fixing bacteria, disease-resistant actinomycetes and armillaria mellea are mixed and inoculated according to the proportion of 3: 1: 1, nitrogen supply is improved, and the occurrence rate of black rot is reduced; 3) activating a GASA stress-resistant gene of the seed hemp through a pulsed electric field (5 kV / cm, 10 microseconds), and enabling the germination rate to reach 88.5% in combination with a calcium signal channel strengthening technology; according to the method, the yield per mu of gastrodia elata is increased by 30%-40%, the gastrodin content is increased by 30% or above, the chemical pesticide dosage is reduced by 55%, the strain passage purity is stable, the method is suitable for large-scale planting in multiple climate areas, and an innovative solution is provided for green production of traditional Chinese medicinal materials.

The invention discloses a genetically engineered bacterium for biosynthesis of gastrodin as well as a construction method and application of the genetically engineered bacterium. According to the genetically engineered bacterium, through overexpression of sucrose permease CscB derived from escherichia coli W, sucrose phosphorylase Basp of bifidobacterium adolescentis, uridine triphosphoryl-glucose-1-phosphate uridine acyl transferase UgpA of bifidobacterium bifidum and UDP-glycosyl transferase RsUGT of hedyotis diffusa, an exogenous path for synthesizing UDP-glucose from sucrose is constructed in a cell, and the UDP-glucose is synthesized into UDP-glucose. And the glycosylation capability of the gastrodin precursor on hydroxybenzyl alcohol is enhanced. The invention also provides a construction method of the genetically engineered bacterium and a method for synthesizing gastrodin through whole-cell catalysis by using the strain and taking p-hydroxybenzyl alcohol and cane sugar as substrates. The genetically engineered bacterium is high in gastrodin synthesis efficiency, the molar conversion rate can reach 97.8%, the highest yield reaches 3.61 g / L, and a new green manufacturing scheme is provided for solving the problems that a traditional gastrodin production method is low in efficiency, high in cost, large in pollution and the like.

The invention discloses a headspace gas chromatography method for detecting ethanol residual quantity in gastrodin. The headspace gas chromatography method comprises the following steps: (1) preparing a test solution; preparing a reference solution; (3) performing chromatographic determination; and (4) calculating a result. According to the headspace gas chromatography method for detecting the residual amount of ethanol in gastrodin, the headspace equilibrium temperature and time are optimized, a chromatographic column with specific polarity and programmed heating conditions are adopted, the ethanol residue detection method special for a gastrodin matrix is established, matrix interference is effectively overcome, and the detection accuracy is high. The method has high sensitivity and high accuracy, realizes exclusive, accurate and reliable quantitative control of ethanol residues in gastrodin, and has strong practicability.

The utility model relates to the technical field of dryers, and discloses a gastrodia elata medicinal material dryer with a temperature adjusting function, which comprises a support frame, a drying box and a heating box, a working motor is arranged at the lower position of the side edge of the drying box, and a fixing bolt for fixing is arranged between a mounting plate and a screen plate. According to the utility model, the temperature sensors are assembled on the two sides in the drying box, so that the temperature sensors can monitor the temperature in the drying box, and meanwhile, the heating condition of the heating resistor is adjusted by connecting a circuit at the position of the heating resistor and adopting a voltage adjusting mode under the arrangement of the heating resistor; the material inlet is obliquely formed in the side edge of the drying box, the stirring rod can be driven to rotate after the working motor works, and the stirring blades are fixed to the outer portion of the stirring rod, so that materials can be stirred under the matching of the stirring rod and the stirring blades, and the drying effect on gastrodia elata medicinal materials is improved.

The application relates to the field of biological medicine, and discloses a glucosyltransferase mutant, a coding gene of the glucosyltransferase mutant, a recombination carrier, a recombination strain, an enzyme preparation, and application of the glucosyltransferase mutant. The glucosyltransferase mutant is a point mutation at the R position of the amino acid sequence shown in SEQ ID NO:1, and 4-8 histidines are added at the N end. A method for preparing gastrodin comprises the following steps: contacting the glucosyltransferase mutant with p-hydroxybenzyl alcohol and uridine diphosphate glucose. The glucosyltransferase mutant provided by the application has higher specificity for the substrate p-hydroxybenzyl alcohol, thereby obviously improving the conversion rate of the conversion of p-hydroxybenzyl alcohol into gastrodin, and having a good industrial application prospect.

This invention belongs to the field of pharmaceutical synthesis technology, specifically relating to a synthetic process for tetraacetyl gastrodin. It involves first preparing tetraacetyl glucose bromide using phosphorus tribromide, then reacting the tetraacetyl glucose bromide with a sodium alkoxide to obtain a condensation product, which is then reduced to yield tetraacetyl gastrodin. Therefore, under anhydrous conditions, the brominated sugar does not undergo hydrolysis, avoiding the loss of the brominated sugar raw material and thus improving the yield.

This invention discloses a method for the efficient synthesis of gastrodin using recombinant Corynebacterium glutamicum, belonging to the fields of genetic engineering, fermentation engineering, and synthetic biology. This invention uses Corynebacterium glutamicum as the host bacterium and knocks out… pobA The gene was expressed, and the engineered strain WN01 / pB6 was constructed using the expression plasmid pEC-XK99E to express cladoid-pyruvate lyase, carboxylic acid reductase, and glycosyltransferase, achieving heterologous synthesis of gastrodin. Further overexpression was then performed. aroG Genes, overexpression of protein-coding genes that activate carboxylic acid reductase sfp The engineered strain WN05 / pB6 was able to synthesize 7.37 g / L of gastrodin at the shake flask level, and the gastrodin yield reached 17.77 g / L at 72 h of batch fermentation in a 5-L fermenter, with a yield of 0.247 g / L / h. This provides a brand-new biomanufacturing platform for the industrial production of gastrodin.

The present application relates to a kind of preparation method of barley and gastrodia elata beverage, belong to food processing technical field.The existing gastrodia elata beverage preparation method has the problems such as low active ingredient extraction efficiency, difficult to synergistically utilize barley functional components, flavor relies on exogenous addition, product is easy to precipitate etc..The present application is by the specific proportion of mixing maturation of barley and gastrodia elata, inoculation includes saccharomyces cerevisiae, lactobacillus plantarum and streptococcus thermophilus Compound starter carries out solid state fermentation, then it is by the compound enzyme preparation including alpha-amylase, beta-glucanase and pectinase enzymolysis and subsequent processing, and beverage is prepared.The method synergistically improves gastrodia elata element and barley beta-glucan enrichment, creates coordinated flavor, and improves product stability.The obtained beverage is mainly used to provide the flavor beverage with the nutritional and health care function of gastrodia elata and barley.

The application provides a method for establishing HPLC fingerprint of Changnyle capsule (I), which is characterized in that 16 active components including gastrodin, danshensu, quercetin-3-O-beta-D-glucose-7-O-beta-D-gentiopicroside, 3'-hydroxy puerarin, puerarin, 3'-methoxy puerarin, puerarin apioside, paeoniflorin, daidzin, rosmarinic acid, shikonin, isoquercitrin, ononin, daidzein, salvianolic acid B and calycosin are used as reference substances, HPLC is adopted, methanol-acetonitrile-0.5vol% phosphoric acid aqueous solution is used as a mobile phase, different batches of samples are detected, and finally the fingerprint of Changnyle capsule (I) is obtained. The fingerprint can comprehensively represent the main components in Changnyle capsule (I), thereby being beneficial to the quality control of Changnyle capsule (I).

The present application relates to the technical field of medicine, and discloses application of gastrodin in preparation of medicine for preventing and treating radioactive liver injury, wherein the radioactive liver injury is liver injury caused by ionizing radiation exposure. It is found that the gastrodin can effectively intervene in specific mechanism of the radioactive liver injury, significantly reduce the mortality of the radioactive liver injury mice, reverse the hepatic veno-occlusive disease and the core pathological features of liver fibrosis of the radioactive liver injury, and more specifically treat the radioactive liver injury. On the other hand, the gastrodin can be used before radiation exposure to prevent serious radioactive liver injury after radiation exposure.

The invention belongs to the technical field of microbial fermentation, and particularly relates to a plant lactobacillus YSH08 and application thereof in preparation of gastrodia elata leavening with the efficacy of reducing blood sugar and blood fat. The gastrodia elata fermentation product is prepared by inoculating a gastrodia elata fermentation agent and fermenting; wherein the leavening agent is prepared from a plant lactobacillus YSH08 or a kluyveromyces marxianus Hz09. The gastrodia elata leavening obtained by the method has the effects of reducing blood sugar and blood fat; in addition, an in-vitro digestion experiment shows that saponin, polyphenol, gastrodin, parishin A, parishin B and parishin C in the fermented product all have very good biological accessibility.

The invention discloses kluyveromyces marxianus SWU-392 and application thereof, and belongs to the technical field of microorganisms. In order to solve the problems that gastrodia elata is limited to be foodstuff due to the fact that the gastrodia elata contains p-methylphenol, hexanal and other bad flavor substances, and flavor improvement and active ingredient retention are difficult to consider in the prior art, yeast with the preservation number of GDMCC No: 67309 is applied to gastrodia elata fermentation, and the gastrodia elata is fermented in a solid state at the constant temperature of 30 DEG C for 4 days. Poor substances such as p-methylphenol and aldehydes in gastrodia elata are effectively degraded, the content of flower and fruit fragrance type flavor substances such as esters is remarkably increased, and meanwhile the content of gastrodin and p-hydroxybenzyl alcohol is slightly increased. The process is mild, safe, free of chemical additives, high in strain adaptability and easy to industrially amplify, collaborative optimization of flavor and efficacy of the gastrodia elata is achieved, and a green and efficient technical scheme is provided for food application of the gastrodia elata.

The invention belongs to the technical field of hyperspectral intelligent detection, and discloses a hyperspectral monitoring and preparation method of eurotium cristatum fermented gastrodia elata, which comprises the following steps: adjusting the water content of dried gastrodia elata or cutting fresh gastrodia elata into square blocks, placing in a fermentation bottle, sterilizing, inoculating eurotium cristatum spore suspension, uniformly mixing, placing in a constant-temperature incubator, and culturing for 0-12 days; regularly sampling and collecting a hyperspectral image, screening characteristic wavelengths by using a CARS-MLR algorithm, establishing a PLS-DA model, and performing pixel-level prediction and visual mapping on indexes such as gastrodin and p-hydroxybenzyl alcohol; gC-MS (gas chromatography-mass spectrometry) volatile substance analysis and sensory evaluation verify that the horse urine smell is remarkably reduced after fermentation, and pleasant fragrances such as mushroom flower fragrance and mint fragrance are enhanced. According to the method, the content of active ingredients such as polysaccharides and polyphenols is remarkably increased, the flavor of the gastrodia elata is improved, online nondestructive testing and intelligent control in the gastrodia elata fermentation process are achieved, and the fermented gastrodia elata product with low peculiar smell, high activity and enhanced functions is obtained.

The invention discloses a traditional preparation process of gastrodia elata line incense, and belongs to the technical field of traditional processes, and the traditional preparation process comprises the following steps: S1, AI-driven flavoring research and development: digitizing dimensions such as aroma concentration and chemical properties, combining a convolutional neural network algorithm, improving the flavoring efficiency by 10 times, shortening the customization response time by 36%, and meanwhile, realizing automatic optimization of an essence formula; the research and development period is shortened by 60%; s2, low-temperature wall-breaking extraction of gastrodia elata: extracting gastrodin by adopting supercritical CO2 extraction (parameters: 35 DEG C and 15 MPa) to avoid high-temperature damage, inclusion of an extract and beta-cyclodextrin to form a heat-resistant microcapsule, and improving the combustion tolerance to 300 DEG C; s3, step-by-step fusion: extracting frankincense resin through absolute ethyl alcohol, mixing the gastrodia elata microcapsules, the frankincense resin and borneol together, and performing manual rolling. Rolling and pressing are still manually carried out, the core material is rolled and formed in cooperation with a mold, and the situation that the incense body is compact due to high pressure is avoided.

The present application relates to the field of biological medical technology, in particular to a traditional Chinese medicine composition as GSK-3beta inhibitor and preparation and application thereof. The traditional Chinese medicine composition as GSK-3beta inhibitor comprises gastrodin and ganoderma triterpene, and the mass ratio of gastrodin and ganoderma triterpene is (1-4):1. The traditional Chinese medicine composition provided by the present application can inhibit the overexpression of GSK-3beta in AD model, thereby inhibiting the overphosphorylation of Tau protein and playing a protective role on Alzheimer's disease. In addition, the traditional Chinese medicine composition provided by the present application contains gastrodin and ganoderma triterpene, which are homoeopathic traditional Chinese medicine efficacy components, greatly improving the safety of the drug, and providing a train of thought for the application of the traditional Chinese medicine composition as a functional food.

The invention belongs to the technical field of functional foods, and particularly relates to a gastrodia elata-dogwood composite health-care beverage and a preparation method thereof. Specifically, the gastrodia elata extract and the dogwood extract are respectively extracted, then a sweetening agent (such as white granulated sugar) is added for compounding to finally obtain the gastrodia elata and dogwood composite health-care beverage, and the beverage is light pink, clear and transparent, free of sediment, good in storage stability, harmonious in flavor, appropriate in taste, free of abrupt external taste, and capable of being eaten by people. Meanwhile, the gastrodia elata beverage is rich in functional components such as gastrodin, gastrodia elata polysaccharide, loganin and the like, and has a good health-care value. Meanwhile, the gastrodia elata and dogwood composite health-care beverage is simple in preparation method, low in cost and easy for large-scale production, so that deep processing of agricultural products such as gastrodia elata and dogwood is facilitated, and the gastrodia elata and dogwood composite health-care beverage has good practical application value.

The application provides a high-stability gastrodin production process, which takes gastrodin tetraacetate as a starting material, and sequentially undergoes pentaacetylation reaction, centrifugation and drying to obtain a gastrodin pentaacetate intermediate; then gradient saponification refining is adopted, that is, preliminary hydrolysis is first carried out by using a low-concentration lye, and then deep saponification is carried out by using a high-concentration lye; after saponification, the product is subjected to centrifugation, drying and crushing to obtain a finished product; an ultrasonic-assisted crystallization step is added after saponification, and an inert gas atmosphere coating is carried out after crushing. The glycosidic bond breakage caused by strong alkali is effectively avoided through gradient saponification, and the key problems of low product purity and poor stability in the existing process are solved in combination with ultrasonic crystallization and inert gas protection. The obtained gastrodin product has the remarkable characteristics of high purity, few impurities and excellent stability, and has the following indexes: purity ≥ 99.1%, related substances ≤ 0.45%, and content decrease rate ≤ 0.8% after 18 months of accelerated test.

The invention discloses a pharmaceutical composition for promoting neuron neogenesis and a method for promoting neuron neogenesis by using a gastrodia elata extract or an adenosine analogue. The gastrodia elata extract comprises gastrodin, gastrodigenin, a parishins compound, N6-(4-hydroxybenzyl) adenosine (T1-11) and 4-hydroxybenzaldehyde (4-hydroxybenzaldehyde). The invention also discloses a method for promoting neuron neogenesis by using the gastrodia elata extract or the adenosine analogue. The gastrodia elata extract provided by the invention has aging-retarding activity on nerve cells and can induce mouse hippocampal neuronal neogenesis.

This invention belongs to the field of pharmaceutical formulation technology, specifically disclosing a gastrodin transdermal patch and its preparation method. The patch consists of a backing layer, a drug-containing matrix layer, and an anti-adhesive layer, using gastrodin as the active ingredient. It employs HP-β-CD inclusion technology, combined with an HPMC and PVP polymer backbone and an oleic acid-menthol composite transdermal enhancement system. The preparation utilizes solvent casting and low-temperature vacuum drying processes, which are mild and controllable. This invention effectively solves the problems of difficult transdermal absorption, easy recrystallization, and low bioavailability of gastrodin. The resulting patch has excellent adhesion, no skin irritation, high transdermal penetration, and good skin biocompatibility. It can efficiently cross the blood-brain barrier, regulate the neuroinflammatory microenvironment in the brain, significantly inhibit the colonization and progression of non-small cell lung cancer brain metastases, and strongly reverse tumor-related anxiety and depression-like mood disorders. It provides a novel drug delivery regimen with great clinical translational potential for the non-invasive synergistic treatment of brain tumors and their associated mood disorders.

The utility model relates to gastrodia elata medicinal material crushing technical field discloses gastrodia elata medicinal material crushing device convenient for feeding, including support base, both sides of support base all are fixed with support board, the top of inside support board is hinged and is installed with the crushing cylinder through the pivot, the inside installation of cylinder cover has the observation glass, the inside middle position of observation glass is swingly provided with the rotary rod, and the bottom of rotary rod installs the scraper, the top of rotary rod installs the rotation block. The utility model discloses the crushing cylinder uses the cylinder cover of upper side as the flip, and the crushing cylinder is opened conveniently, and the gastrodia elata is put into the inside crushing cylinder and is crushed, and the inside setting of cylinder cover has scraper, observation glass and rotary rod etc. component, then it is convenient to observe the gastrodia elata division crushing state in the process of gastrodia elata crushing, realizes this crushing device convenient for feeding and observes the crushing process, and the practicality is stronger.

This invention discloses a pulverizing and processing device for gastrodin extraction, relating to the field of gastrodin extraction technology. It includes a mechanism cylinder and a support frame fixedly connected to the bottom outer side of the mechanism cylinder. A conical cover is fixedly connected to the upper side of the mechanism cylinder, and a sealing cover is detachably connected to the upper side of the conical cover. A first pulverizing mechanism is provided inside the sealing cover, and a second pulverizing mechanism is provided inside the mechanism cylinder. The mechanism cylinder includes a motor, a transmission rod fixedly connected to the motor output end, and a lifting assembly installed at the bottom inner side of the mechanism cylinder. The lifting assembly drives the motor to move up and down. A placement plate is rotatably connected to the upper end of the transmission rod. This pulverizing and processing device for gastrodin extraction uses a cooler to cool the upper side of the placement plate and the inside of the grinding cylinder, ensuring that the gastrodin pieces remain at a low temperature during the pulverizing process, thus avoiding heat accumulation that could affect the pulverizing efficiency.

The utility model relates to a gastrodia elata shaping, processing and compacting device, and belongs to the technical field of gastrodia elata processing. Comprising an L-shaped base, supporting grooves, a discharging disc, a limiting assembly, a moving assembly, an electric cylinder and a pressing plate, a supporting plate is installed on the L-shaped base, the multiple supporting grooves are formed in the top of the supporting plate at equal intervals, the discharging disc is movably installed on the supporting grooves through the limiting assembly, a transverse plate is installed on the top of the L-shaped base, and the moving assembly is installed at the bottom of the transverse plate; the electric cylinder is installed on the moving assembly, and a pressing plate is installed at the top of the electric cylinder. The pressing plate is installed on the moving assembly through the electric cylinder, the pressing plate can be moved conveniently, the gastrodia elata in the discharging discs is compacted and shaped in sequence, when the gastrodia elata of one discharging disc is compacted and shaped, feeding and discharging can be conducted on the other discharging discs, in the feeding and discharging process, the pressing plate can work all the time, and the machining efficiency is improved.

The invention belongs to the technical field of biology, and particularly relates to an AtUGT72B1 glycosyl transferase mutant and application of the AtUGT72B1 glycosyl transferase mutant in gastrodin synthesis. The technical problem to be solved by the invention is that the efficiency of catalyzing the conversion of p-hydroxybenzyl alcohol into glycosyl transferase of gastrodin is low at present. According to the technical scheme, the AtUGT72B1 glycosyl transferase mutant is obtained by mutating at least one amino acid in the 83rd position of glutamic acid, the 81st position of threonine, the 144th position of valine or the 310th position of isoleucine. The mutant disclosed by the invention can greatly improve the gastrodin generation efficiency of a microbial fermentation method.

The invention relates to the technical field of medicinal material drying equipment, in particular to a gastrodia elata processing and drying device and method. The drying device comprises a drying box and a driving mechanism, two groups of plates are arranged in the drying box, and each group of plates comprises a supporting plate and a filling plate; a gap for accommodating gastrodia elata is formed between the two groups of plates; the supporting plate is provided with at least three long grooves, the filling plate is provided with protruding strips used for filling the long grooves, and the protruding strips are provided with needle tips puncturing the epidermis of gastrodia elata. The driving mechanism is used for driving the two plates to get close to each other to extrude gastrodia elata. According to the gastrodia elata separating device, the function that gastrodia elata is smoothly separated after being extruded is achieved, the effect of reducing the contact area between the gastrodia elata and a supporting tool is achieved by controlling a filling plate to be separated from the gastrodia elata firstly, and due to the fact that a long groove is formed in a supporting plate, the gastrodia elata can be supported through the supporting plate, and adhesion between the gastrodia elata and the supporting plate can be avoided; therefore, the problem that pectin is separated out in the flattening process of the gastrodia elata and is adhered to the supporting tool is solved.

The application discloses a method for simultaneously purifying gastrodin, bartzol and gastrodia elata polysaccharide from gastrodia elata, which comprises the following steps: crushing, extracting, neutral or weak polar macroporous resin adsorbing, polar macroporous resin adsorbing, gradient elution, membrane separation and the like, so as to obtain gastrodia elata extracts rich in gastrodin, bartzol and gastrodia elata polysaccharide respectively. The method uses gastrodia elata as raw material, has simple extraction and separation and purification methods, uses green and non-polluted solvents, has low cost, and through the adsorption of different polar resins, gradient elution and membrane separation and the like, the gastrodia elata extracts rich in gastrodin, bartzol and gastrodia elata polysaccharide are obtained respectively, the effect of squeezing dry and taking all is achieved, the utilization rate is high, the utilization value of gastrodia elata is improved, and the method has wide application prospect.

This invention relates to the field of traditional Chinese medicine preparation technology, and discloses a fingerprint-standardized traditional Chinese medicine composition for relieving myopia, comprising: the composition is obtained by extracting Gastrodia elata, Paeonia lactiflora and Salvia miltiorrhiza, and contains gastrodin, paeoniflorin and salvianolic acid B; the composition contains micelles formed by endogenous components, with gastrodin, paeoniflorin and salvianolic acid B distributed in the hydrophobic centers inside the micelles; the composition induces micelle interface contraction by adjusting the polarity of the extract and the temperature gradient, thereby sealing the active components within the micelles to cut off their thermodynamic rearrangement pathways, and the fingerprint spectrum similarity of its common characteristic peaks is not less than 0.90. This invention utilizes the micelle interface locking mechanism to fix the spatial distribution of active components, solves the problem of ratio dispersion caused by reverse analysis of active ingredients in the extraction stage, and ensures that the composition has a constant biological activity output in relieving ciliary muscle spasm and improving retinal microcirculation.

The invention belongs to the technical field of medicine detection, and particularly relates to a method for detecting the content of effective components in seven-ingredient gastrodia elata medicinal liquor, the seven-ingredient gastrodia elata medicinal liquor is prepared from gastrodia elata, codonopsis pilosula, kadsura longepedunculata, poria cocos, fructus lycii, pericarpium citri reticulatae and radix polygoni multiflori preparata, has the effects of tonifying the kidney and soothing the nerves, and is used for neurasthenia, insomnia and dreaminess and the like. The method for detecting the content of the effective components of the seven-ingredient rhizoma gastrodiae medicinal liquor comprises the step of determining the content of gastrodin in a rhizoma gastrodiae medicinal material, the content of stilbene glucoside in a radix polygoni multiflori preparata medicinal material and the content of deoxyschizandrin in a kadsura longepedunculata medicinal material by adopting a high performance liquid chromatography. According to the method, a quantitative analysis method of three main active components is established through high performance liquid chromatography, a quality control system of the seven-ingredient gastrodia elata medicinal liquor is perfected, the adopted detection method is scientific and reasonable, high in specificity, high in accuracy and good in reproducibility, the quality of the seven-ingredient gastrodia elata medicinal liquor can be comprehensively and effectively controlled, and the curative effect and safety of the product are ensured.

The invention belongs to the technical field of plant polysaccharides, and particularly discloses gastrodia elata oligosaccharide, a preparation method thereof and application of the gastrodia elata oligosaccharide in preparation of antioxidant products. The gastrodia elata oligosaccharide has a structure as shown in a formula (I), is a novel-structure pH-neutral uniform oligosaccharide separated from a natural traditional Chinese medicinal material gastrodia elata and has excellent antioxidant activity, the DPPH free radical scavenging rate reaches about 70% when the concentration is 0.6 mg / mL and is 72.5% when the concentration is 1.0 mg / mL, the antioxidant activity of the gastrodia elata oligosaccharide is obviously higher than that of crude gastrodia elata polysaccharide (58.3%) and a simple purified product (65.1%) of the crude gastrodia elata polysaccharide and is close to that of Vc (78.6%), and the antioxidant activity of the gastrodia elata oligosaccharide is obviously higher than that of the crude gastrodia elata polysaccharide. The product is expected to be developed into a novel antioxidant product.

The application discloses a kind of ginseng root decoction pieces fingerprint construction method and fingerprint and application, including the ginseng root decoction pieces fingerprint construction that meets the quality requirement of ginseng root dispensing granule, the fingerprint includes ginseng root factor, p-hydroxybenzyl alcohol, ballysen glycoside, ballysen glycoside B, ballysen glycoside C and ballysen glycoside E six Mark peaks, the control fingerprint generated by common peak mode can be used as comparison, whether the ginseng root decoction pieces fingerprint meets the requirement is judged, when ginseng root decoction pieces fingerprint similarity is not less than 0.76, the ginseng root dispensing granule fingerprint prepared similarity is not less than 0.90.The application solves the unstable problem of ginseng root decoction pieces quality that meets the quality requirement of ginseng root dispensing granule.

The application belongs to the technical field of plant polysaccharide, and particularly discloses a Gastrodia elata oligosaccharide, a preparation method thereof and an application of the Gastrodia elata oligosaccharide in preparing an antioxidant product. The Gastrodia elata oligosaccharide has a structure as shown in formula (I), is a new type of pH neutral homogeneous oligosaccharide separated from natural Chinese medicinal material Gastrodia elata, and has excellent antioxidant activity. When the concentration of the Gastrodia elata oligosaccharide is 0.6 mg / mL, the DPPH free radical scavenging rate reaches about 70%, and when the concentration is 1.0 mg / mL, the DPPH free radical scavenging rate is 72.5%, which is significantly higher than that of Gastrodia elata crude polysaccharide (58.3%) and a simple purified product of the Gastrodia elata crude polysaccharide (65.1%), and is close to the antioxidant activity of Vc (78.6%), so that the Gastrodia elata oligosaccharide is expected to be developed into a new antioxidant product.