128 results about "Gastrodin" patented technology

The invention relates to the technical field of biomedicine, in particular to application of gastrodin in prevention and treatment of Alzheimer's disease. The invention provides application of gastrodin in prevention and treatment of the Alzheimer's disease, and provides a new way for preventing and treating the Alzheimer's disease. And gastrodin is from gastrodia elata, is used as a mild medicinal and edible raw material component, and has no toxic or side effect on the body. Gastrodin is adopted as a raw material, and a series of experimental studies prove that gastrodin has an inhibitory effect on GSK-3beta / P38MAPK, and can obviously improve various pathological changes of the Alzheimer's disease caused by APOE4. The invention verifies the effect of gastrodin on the Alzheimer's disease high-risk factor APOE4, and provides a brand-new technical scheme and theoretical basis for developing medicines, health-care foods or nutritional supplements which take gastrodin as a core component and are used for preventing or treating Alzheimer's disease.

The invention discloses a high-yield gastrodia elata planting method based on Tilia brachypoda fungus material optimization and seed ramie stress resistance activation. According to the high-yield gastrodia elata planting method based on Tilia brachypoda fungus material optimization and seed ramie stress resistance activation, yield increase and quality improvement are achieved through fungus material composite strengthening treatment and a composite strain synergistic inoculation technology. The method comprises the following steps: (1) fermenting a tilia brachypoda substrate by lactobacillus plantarum and soaking in a nano-composite nutrient solution to form a high-activity bacterial material; (2) nitrogen-fixing bacteria, disease-resistant actinomycetes and armillaria mellea are mixed and inoculated according to the proportion of 3: 1: 1, nitrogen supply is improved, and the occurrence rate of black rot is reduced; 3) activating a GASA stress-resistant gene of the seed hemp through a pulsed electric field (5 kV / cm, 10 microseconds), and enabling the germination rate to reach 88.5% in combination with a calcium signal channel strengthening technology; according to the method, the yield per mu of gastrodia elata is increased by 30%-40%, the gastrodin content is increased by 30% or above, the chemical pesticide dosage is reduced by 55%, the strain passage purity is stable, the method is suitable for large-scale planting in multiple climate areas, and an innovative solution is provided for green production of traditional Chinese medicinal materials.

The invention discloses an aspergillus cristatus strain capable of increasing gastrodin content and application of the aspergillus cristatus strain, and belongs to the technical field of solid state fermentation engineering. The Aspergillus cristatus strain ASE-4 is preserved in the China Center for Type Culture Collection, and the preservation number of the Aspergillus cristatus strain ASE-4 is CCTCC (China Center for Type Culture Collection) NO: M20241392. By optimizing the fermentation method and modeling through the ANN-GA algorithm (R2 = 0.92), the gastrodin content (50% higher than that of an unfermented group) and the polysaccharide and total flavone content are remarkably improved. The strain and the fermentation method provided by the invention solve the problem that the gastrodin content is reduced in the prior art, and are suitable for development of high-added-value gastrodia elata products.

The invention discloses sterilization equipment for gastrodin injection, and belongs to the field of sterilization equipment, the sterilization equipment comprises a sterilization tank, and a circular plate is rotatably mounted on the inner cavity wall of the sterilization tank; the device further comprises a circulating assembly, the circulating assembly comprises a fixed pipe rotationally installed at the top of the circular plate, a groove communicated with the fixed pipe is formed in the top of the circular plate, a protection frame is fixedly installed in an inner cavity of the fixed pipe, a gear rotationally installed with the protection frame is arranged in the groove, and the circular plate is fixedly installed in the groove. Teeth are evenly installed in an inner cavity of the groove, the gear and the adjacent teeth are installed in a meshed mode, and fan blades rotationally installed on the protection frame are arranged in the fixing pipe. Water drops formed outside the glass bottle can be blown off and recycled while water vapor circularly flows, the temperature uniformity in the sterilization process can be remarkably improved, the problem of condensate water residues can be effectively solved, and high quality and high safety of a final product are ensured.

The invention provides an application of gastrodin in preparation of a medicine for treating peripheral neuropathy. According to the application disclosed by the invention, gastrodin can inhibit neuroinflammation mediated by microglia by inhibiting activation of an NF-kB / NLRP3 inflammasome signal channel, so that release of inflammatory factors is reduced, and neuropathy induced by bortezomib is relieved.

The invention discloses fresh rhizoma gastrodiae preserved fruits and a preparation method thereof. The preparation method comprises the following steps: (1) pretreatment; (2) freeze thawing treatment; (3) steaming with honey; (4) performing ultrahigh-pressure honey processing and sugar permeation treatment; (5) coating treatment; (6) drying at variable temperature; and (7) packaging and sterilizing. According to the method disclosed by the invention, citric acid color protection is combined with coating liquid treatment, so that browning is effectively inhibited, and the color and luster preservation period is prolonged; a freeze-thawing combined ultrahigh pressure honey processing synergistic process is innovatively adopted, so that the sugar soaking time is greatly shortened, the production efficiency is improved, efficient and uniform sugar soaking can be realized, the sugar distribution is uniform, and the taste is better; the variable-temperature drying process reduces loss of heat-sensitive nutritional ingredients (such as gastrodin), the unique flavor of the fresh gastrodia elata is reserved to the maximum extent, and the honey gradually permeates and is fused with the flavor of the gastrodia elata to form a composite flavor, so that the product has both leisure flavor and health value.

The invention discloses a genetically engineered bacterium for biosynthesis of gastrodin as well as a construction method and application of the genetically engineered bacterium. According to the genetically engineered bacterium, through overexpression of sucrose permease CscB derived from escherichia coli W, sucrose phosphorylase Basp of bifidobacterium adolescentis, uridine triphosphoryl-glucose-1-phosphate uridine acyl transferase UgpA of bifidobacterium bifidum and UDP-glycosyl transferase RsUGT of hedyotis diffusa, an exogenous path for synthesizing UDP-glucose from sucrose is constructed in a cell, and the UDP-glucose is synthesized into UDP-glucose. And the glycosylation capability of the gastrodin precursor on hydroxybenzyl alcohol is enhanced. The invention also provides a construction method of the genetically engineered bacterium and a method for synthesizing gastrodin through whole-cell catalysis by using the strain and taking p-hydroxybenzyl alcohol and cane sugar as substrates. The genetically engineered bacterium is high in gastrodin synthesis efficiency, the molar conversion rate can reach 97.8%, the highest yield reaches 3.61 g / L, and a new green manufacturing scheme is provided for solving the problems that a traditional gastrodin production method is low in efficiency, high in cost, large in pollution and the like.

The present invention discloses Armillaria mellea, a fungal agent containing Armillaria mellea, and its application, and relates to the field of microorganisms. The present invention provides any of the following Armillaria mellea strains, all of which are deposited in the General Microbiology Center of the China Culture Collection Administration of Microorganisms: a) Armillaria mellea Armillaria sp. , the deposit number is CGMCC No.41696; b) Armillaria mellea Armillaria sp. , the deposit number is CGMCC No.41697; c) Armillaria mellea Armillaria sp. The three Armillaria mellea strains provided by the present invention can establish a good symbiotic relationship with Gastrodia elata. The use of the Armillaria mellea strains in accompanying sowing or planting of Gastrodia elata can improve the yield and quality of Gastrodia elata and increase the content of polysaccharides, gastrodin and p-hydroxybenzyl alcohol in Gastrodia elata.

In various embodiments, provided herein are host cells, methods, and pharmaceutical compositions comprising gastrodin, wherein the gastrodin is produced by a genetically modified plant or plant cell, fungal cell, yeast cell, insect cell, or bacterial cell. In certain embodiments, the present disclosure provides methods and compositions for the production of gastrodin. In yet other embodiments, the present disclosure provides enhanced cells and methods for producing gastrodin.

The invention discloses a headspace gas chromatography method for detecting ethanol residual quantity in gastrodin. The headspace gas chromatography method comprises the following steps: (1) preparing a test solution; preparing a reference solution; (3) performing chromatographic determination; and (4) calculating a result. According to the headspace gas chromatography method for detecting the residual amount of ethanol in gastrodin, the headspace equilibrium temperature and time are optimized, a chromatographic column with specific polarity and programmed heating conditions are adopted, the ethanol residue detection method special for a gastrodin matrix is established, matrix interference is effectively overcome, and the detection accuracy is high. The method has high sensitivity and high accuracy, realizes exclusive, accurate and reliable quantitative control of ethanol residues in gastrodin, and has strong practicability.

The present application provides gastrodia elata (Blume) alkaloids PFT201A, PFT201B and PFT203 and a preparation method thereof, and application thereof in the fields of cosmetics and medicine. Gastrodia elata Gastrodia elata (Blume) alkaloids PFT201A, PFT201B and PFT203 significantly promote the secretion of collagen type II in human dermal fibroblasts-adult (HDFa) at an extremely low concentration (0.1 μ M), and have no cytotoxicity at the concentration. These compounds can be used for preparing anti-aging drugs or skin care products.

The utility model relates to the technical field of dryers, and discloses a gastrodia elata medicinal material dryer with a temperature adjusting function, which comprises a support frame, a drying box and a heating box, a working motor is arranged at the lower position of the side edge of the drying box, and a fixing bolt for fixing is arranged between a mounting plate and a screen plate. According to the utility model, the temperature sensors are assembled on the two sides in the drying box, so that the temperature sensors can monitor the temperature in the drying box, and meanwhile, the heating condition of the heating resistor is adjusted by connecting a circuit at the position of the heating resistor and adopting a voltage adjusting mode under the arrangement of the heating resistor; the material inlet is obliquely formed in the side edge of the drying box, the stirring rod can be driven to rotate after the working motor works, and the stirring blades are fixed to the outer portion of the stirring rod, so that materials can be stirred under the matching of the stirring rod and the stirring blades, and the drying effect on gastrodia elata medicinal materials is improved.

The application relates to the field of biological medicine, and discloses a glucosyltransferase mutant, a coding gene of the glucosyltransferase mutant, a recombination carrier, a recombination strain, an enzyme preparation, and application of the glucosyltransferase mutant. The glucosyltransferase mutant is a point mutation at the R position of the amino acid sequence shown in SEQ ID NO:1, and 4-8 histidines are added at the N end. A method for preparing gastrodin comprises the following steps: contacting the glucosyltransferase mutant with p-hydroxybenzyl alcohol and uridine diphosphate glucose. The glucosyltransferase mutant provided by the application has higher specificity for the substrate p-hydroxybenzyl alcohol, thereby obviously improving the conversion rate of the conversion of p-hydroxybenzyl alcohol into gastrodin, and having a good industrial application prospect.

The invention belongs to the technical field of medicines, and discloses a gastrodia elata microbial fermentation product as well as a preparation method and application thereof. According to the invention, three aspergillus oryzae are utilized to successfully carry out microbial fermentation on gastrodia elata, the gastrodia elata microbial fermentation product of which the content of active ingredients gastrodin and p-hydroxybenzyl alcohol is obviously increased is obtained, and the pharmacological action of the gastrodia elata microbial fermentation product on treating or improving oligoasthenospermia is verified through an oligoasthenospermia mouse model; results show that the gastrodia elata microbial fermentation product has better effects of improving sperm concentration, enhancing sperm motility and rapidly moving forwards, can be used as an active ingredient of a medicine for treating or improving oligospermia and asthenospermia, and has wide application.

The invention relates to a gastrodia elata glycosyl transferase GaUGT131 gene and application thereof in preparation of gastrodin, and belongs to the technical field of biology. The nucleotide sequence of the gastrodia elata glycosyl transferase GaUGT131 gene is as shown in SEQ ID NO. 1, and the total length of the sequence is 1524 bp; the amino acid sequence of the encoded protein is shown as SEQ ID NO.2, and 508 amino acid residues are encoded. The gastrodia elata glycosyl transferase GaUGT131 gene can be used as a biosynthesis regulation gene of gastrodin, is applied to preparation of gastrodin, and is remarkable in application prospect and easy to popularize and apply.

This invention belongs to the field of pharmaceutical synthesis technology, specifically relating to a synthetic process for tetraacetyl gastrodin. It involves first preparing tetraacetyl glucose bromide using phosphorus tribromide, then reacting the tetraacetyl glucose bromide with a sodium alkoxide to obtain a condensation product, which is then reduced to yield tetraacetyl gastrodin. Therefore, under anhydrous conditions, the brominated sugar does not undergo hydrolysis, avoiding the loss of the brominated sugar raw material and thus improving the yield.

This invention discloses a method for the efficient synthesis of gastrodin using recombinant Corynebacterium glutamicum, belonging to the fields of genetic engineering, fermentation engineering, and synthetic biology. This invention uses Corynebacterium glutamicum as the host bacterium and knocks out… pobA The gene was expressed, and the engineered strain WN01 / pB6 was constructed using the expression plasmid pEC-XK99E to express cladoid-pyruvate lyase, carboxylic acid reductase, and glycosyltransferase, achieving heterologous synthesis of gastrodin. Further overexpression was then performed. aroG Genes, overexpression of protein-coding genes that activate carboxylic acid reductase sfp The engineered strain WN05 / pB6 was able to synthesize 7.37 g / L of gastrodin at the shake flask level, and the gastrodin yield reached 17.77 g / L at 72 h of batch fermentation in a 5-L fermenter, with a yield of 0.247 g / L / h. This provides a brand-new biomanufacturing platform for the industrial production of gastrodin.

The present application relates to a kind of preparation method of barley and gastrodia elata beverage, belong to food processing technical field.The existing gastrodia elata beverage preparation method has the problems such as low active ingredient extraction efficiency, difficult to synergistically utilize barley functional components, flavor relies on exogenous addition, product is easy to precipitate etc..The present application is by the specific proportion of mixing maturation of barley and gastrodia elata, inoculation includes saccharomyces cerevisiae, lactobacillus plantarum and streptococcus thermophilus Compound starter carries out solid state fermentation, then it is by the compound enzyme preparation including alpha-amylase, beta-glucanase and pectinase enzymolysis and subsequent processing, and beverage is prepared.The method synergistically improves gastrodia elata element and barley beta-glucan enrichment, creates coordinated flavor, and improves product stability.The obtained beverage is mainly used to provide the flavor beverage with the nutritional and health care function of gastrodia elata and barley.

The invention discloses a method for researching an influence mechanism of gastrodin on anxiety-like behaviors after sleep deprivation, and relates to the technical field of pharmacology and behavioral psychology. Comprising the following steps: selecting an ICR male mouse, establishing a sleep deprivation model through an improved multi-platform water environment method, and observing the influence of the expression level of autophagy related protein of the model group mouse on the anxiety-like behavior of the mouse; the method comprises the following specific steps: S1, experiment preparation and reagent preparation; s2, experimental animal grouping; s3, establishing a sleep deprivation model; s4, performing a sleep deprivation experiment and dosing; s5, performing open field experiment on the mouse; s6, performing an elevated cross maze experiment on the mouse; and S7, performing experimental statistics. According to the application, the influence of gastrodin on anxiety-like behaviors of mice after sleep deprivation is systematically discussed for the first time, the action mechanism of the gastrodin is disclosed from the perspective of autophagy related protein, and a new perspective is provided for clinical application of the gastrodin; the mouse sleep deprivation model is successfully established through an improved multi-platform water environment method, and the method is simple and easy to implement and high in repeatability.

The application provides a method for establishing HPLC fingerprint of Changnyle capsule (I), which is characterized in that 16 active components including gastrodin, danshensu, quercetin-3-O-beta-D-glucose-7-O-beta-D-gentiopicroside, 3'-hydroxy puerarin, puerarin, 3'-methoxy puerarin, puerarin apioside, paeoniflorin, daidzin, rosmarinic acid, shikonin, isoquercitrin, ononin, daidzein, salvianolic acid B and calycosin are used as reference substances, HPLC is adopted, methanol-acetonitrile-0.5vol% phosphoric acid aqueous solution is used as a mobile phase, different batches of samples are detected, and finally the fingerprint of Changnyle capsule (I) is obtained. The fingerprint can comprehensively represent the main components in Changnyle capsule (I), thereby being beneficial to the quality control of Changnyle capsule (I).

The invention discloses a detection method for determining a residual solvent in gastrodin by headspace sampling-gas chromatography. The detection method comprises the following steps: (1) preparing a reference substance solution containing the residual solvent to be detected; (2) preparing a test solution containing gastrodin; and (3) injecting the reference solution and the test solution into a gas chromatograph in a headspace sampling manner, and calculating the content of the residual solvent by peak area according to an external standard method. The diluent is used for preparing a reference substance solution and a test solution, a sample is exposed in a gas phase space in a headspace sampling mode, the response of volatile substances is improved, meanwhile, all volatile low-boiling-point to-be-detected residual solvents are effectively captured and detected in a gradient chromatographic column heating mode, the detection method is simple, and the detection efficiency is high. The method has the advantages of high specificity, high precision and high sensitivity, can simultaneously detect a plurality of residual solvents to be detected, greatly saves the detection time and cost, and is beneficial to improving the quality monitoring effect of gastrodin drugs.

The present application relates to the technical field of medicine, and discloses application of gastrodin in preparation of medicine for preventing and treating radioactive liver injury, wherein the radioactive liver injury is liver injury caused by ionizing radiation exposure. It is found that the gastrodin can effectively intervene in specific mechanism of the radioactive liver injury, significantly reduce the mortality of the radioactive liver injury mice, reverse the hepatic veno-occlusive disease and the core pathological features of liver fibrosis of the radioactive liver injury, and more specifically treat the radioactive liver injury. On the other hand, the gastrodin can be used before radiation exposure to prevent serious radioactive liver injury after radiation exposure.

The invention relates to a difunctional gastrodia elata glycosyl transferase GaUGT015 gene and application thereof in preparation of gastrodin and arbutin, and belongs to the technical field of biology. The nucleotide sequence of the difunctional glycosyl transferase GaUGT015 gene of the gastrodia elata is shown as SEQ ID NO.1, and the total length of the sequence is 1407 bp; the amino acid sequence of the encoded protein is as shown in SEQ ID NO.2, and 469 amino acid residues are encoded. The gastrodia elata bifunctional glycosyl transferase GaUGT015 gene disclosed by the invention can be used as a biosynthesis regulation gene of gastrodin and arbutin, is applied to preparation of gastrodin and arbutin, and is remarkable in application prospect and easy to popularize and apply.

The invention relates to the technical field of processing and preparation of medicinal and edible drinks, in particular to gastrodia elata Pu'er tea and a preparation method thereof. The preparation method sequentially comprises the following steps: treating crushed gastrodia elata decoction pieces with cellulase, and filtering to obtain first supernate and first decoction dregs; performing water extraction and filtration on the first medicine residue to obtain a second supernatant and a second medicine residue, and performing ethanol hot-dipping extraction on the second medicine residue twice to obtain an extracting solution; combining the extracting solution and the two supernatants, concentrating, adding povidone K30, stirring, performing ultrafiltration, and concentrating the permeate to obtain a gastrodia elata extracting concentrated solution; drying, crushing and activating Pu'er ripe tea, soaking in hot water for three times, combining tea soup, and filtering to obtain a Pu'er tea extracting solution; and mixing the two extracting solutions, adding maltitol, uniformly mixing, and spray-drying to obtain the gastrodia elata Pu'er tea powder. According to the technical scheme, the technical problem that gastrodia elata Pu'er tea in the prior art is not ideal in taste and appearance can be solved, the eating convenience is improved, the product is rich in gastrodin and tea polyphenol, and the tea powder is suitable for long-term storage to guarantee quality and large-scale production to guarantee efficiency.

The invention belongs to the technical field of microbial fermentation, and particularly relates to a plant lactobacillus YSH08 and application thereof in preparation of gastrodia elata leavening with the efficacy of reducing blood sugar and blood fat. The gastrodia elata fermentation product is prepared by inoculating a gastrodia elata fermentation agent and fermenting; wherein the leavening agent is prepared from a plant lactobacillus YSH08 or a kluyveromyces marxianus Hz09. The gastrodia elata leavening obtained by the method has the effects of reducing blood sugar and blood fat; in addition, an in-vitro digestion experiment shows that saponin, polyphenol, gastrodin, parishin A, parishin B and parishin C in the fermented product all have very good biological accessibility.

The invention discloses kluyveromyces marxianus SWU-392 and application thereof, and belongs to the technical field of microorganisms. In order to solve the problems that gastrodia elata is limited to be foodstuff due to the fact that the gastrodia elata contains p-methylphenol, hexanal and other bad flavor substances, and flavor improvement and active ingredient retention are difficult to consider in the prior art, yeast with the preservation number of GDMCC No: 67309 is applied to gastrodia elata fermentation, and the gastrodia elata is fermented in a solid state at the constant temperature of 30 DEG C for 4 days. Poor substances such as p-methylphenol and aldehydes in gastrodia elata are effectively degraded, the content of flower and fruit fragrance type flavor substances such as esters is remarkably increased, and meanwhile the content of gastrodin and p-hydroxybenzyl alcohol is slightly increased. The process is mild, safe, free of chemical additives, high in strain adaptability and easy to industrially amplify, collaborative optimization of flavor and efficacy of the gastrodia elata is achieved, and a green and efficient technical scheme is provided for food application of the gastrodia elata.

The invention relates to a gastrodia elata glycosyl transferase GaUGT1156 gene and an application of the gastrodia elata glycosyl transferase GaUGT1156 gene in preparation of gastrodin, and belongs to the technical field of biology. The nucleotide sequence of the gastrodia elata glycosyl transferase GaUGT1156 gene is as shown in SEQ ID NO. 1, and the total length of the sequence is 1422bp; the amino acid sequence of the encoded protein is as shown in SEQ ID NO.2, and 474 amino acid residues are encoded. The gastrodia elata glycosyl transferase GaUGT1156 gene can be used as a biosynthesis regulation gene of gastrodin, is applied to preparation of gastrodin, and is remarkable in application prospect and easy to popularize and apply.

The invention belongs to the technical field of hyperspectral intelligent detection, and discloses a hyperspectral monitoring and preparation method of eurotium cristatum fermented gastrodia elata, which comprises the following steps: adjusting the water content of dried gastrodia elata or cutting fresh gastrodia elata into square blocks, placing in a fermentation bottle, sterilizing, inoculating eurotium cristatum spore suspension, uniformly mixing, placing in a constant-temperature incubator, and culturing for 0-12 days; regularly sampling and collecting a hyperspectral image, screening characteristic wavelengths by using a CARS-MLR algorithm, establishing a PLS-DA model, and performing pixel-level prediction and visual mapping on indexes such as gastrodin and p-hydroxybenzyl alcohol; gC-MS (gas chromatography-mass spectrometry) volatile substance analysis and sensory evaluation verify that the horse urine smell is remarkably reduced after fermentation, and pleasant fragrances such as mushroom flower fragrance and mint fragrance are enhanced. According to the method, the content of active ingredients such as polysaccharides and polyphenols is remarkably increased, the flavor of the gastrodia elata is improved, online nondestructive testing and intelligent control in the gastrodia elata fermentation process are achieved, and the fermented gastrodia elata product with low peculiar smell, high activity and enhanced functions is obtained.

The invention discloses a traditional preparation process of gastrodia elata line incense, and belongs to the technical field of traditional processes, and the traditional preparation process comprises the following steps: S1, AI-driven flavoring research and development: digitizing dimensions such as aroma concentration and chemical properties, combining a convolutional neural network algorithm, improving the flavoring efficiency by 10 times, shortening the customization response time by 36%, and meanwhile, realizing automatic optimization of an essence formula; the research and development period is shortened by 60%; s2, low-temperature wall-breaking extraction of gastrodia elata: extracting gastrodin by adopting supercritical CO2 extraction (parameters: 35 DEG C and 15 MPa) to avoid high-temperature damage, inclusion of an extract and beta-cyclodextrin to form a heat-resistant microcapsule, and improving the combustion tolerance to 300 DEG C; s3, step-by-step fusion: extracting frankincense resin through absolute ethyl alcohol, mixing the gastrodia elata microcapsules, the frankincense resin and borneol together, and performing manual rolling. Rolling and pressing are still manually carried out, the core material is rolled and formed in cooperation with a mold, and the situation that the incense body is compact due to high pressure is avoided.

The present application relates to the field of biological medical technology, in particular to a traditional Chinese medicine composition as GSK-3beta inhibitor and preparation and application thereof. The traditional Chinese medicine composition as GSK-3beta inhibitor comprises gastrodin and ganoderma triterpene, and the mass ratio of gastrodin and ganoderma triterpene is (1-4):1. The traditional Chinese medicine composition provided by the present application can inhibit the overexpression of GSK-3beta in AD model, thereby inhibiting the overphosphorylation of Tau protein and playing a protective role on Alzheimer's disease. In addition, the traditional Chinese medicine composition provided by the present application contains gastrodin and ganoderma triterpene, which are homoeopathic traditional Chinese medicine efficacy components, greatly improving the safety of the drug, and providing a train of thought for the application of the traditional Chinese medicine composition as a functional food.