27 results about "Open field" patented technology

The invention discloses application of an Hspa5 inhibitor in preparation of a medicine for preventing or treating anxiety disorder. An anxiety mouse model is constructed through chronic constraint stress (CRS), and in combination with medial amygdala kernel (MeA) transcriptome sequencing and qPCR verification, it is found that the endoplasmic reticulum molecular chaperone Hspa5 is remarkably up-regulated in the anxiety state. Furthermore, an Hspa5 specific inhibitor HA15 is locally injected into a MeA brain region, so that the anxiety-like behavior induced by the CRS is remarkably improved, and the exploration time of an open field experiment central region, the exploration time of an open arm of an elevated cross labyrinth and the exploration time of a bright box of a bright-dark box experiment are prolonged. The invention discloses the function of Hspa5 as a novel anti-anxiety target for the first time, and provides a direct experimental basis and a transformation direction for developing a novel anti-anxiety drug which is non-monoamine and targets an endoplasmic reticulum homeostasis.

The invention relates to the technical field of mining, in particular to a mining and cutting parameter intelligent optimization method and system based on a small-section open-stope method. The method comprises the following steps: acquiring historical mining case data, and training an index prediction model by using the historical mining case data; geological data of a to-be-mined unit are obtained, and geological feature data are extracted from the geological data; based on the geological feature data, the index prediction model uses a multi-objective optimization algorithm to calculate an optimal mining and cutting parameter scheme set; and selecting an optimal mining and cutting parameter in the optimal mining and cutting parameter scheme set based on the index prediction model and the geological feature data. The problem that in the prior art, mining and cutting parameter calculation based on a small-segment open-field method is not accurate enough is solved, an index prediction model is trained through historical mining case data, a scheme set is generated through multi-target optimization in combination with geological features of a to-be-mined unit, and then adaptive optimal mining and cutting parameters are screened, so that the mining and cutting parameter calculation accuracy is improved. And the accuracy of dynamic calculation of the mining and cutting parameters is greatly improved.

The application relates to the technical field of new uses of medicines, and particularly discloses application of an N-phenylpiperazine derivative in preparation of a medicine for treating Parkinson's disease, wherein the N-phenylpiperazine derivative is HS. + The N-phenylpiperazine derivative HS provided by the application can significantly improve the survival rate of SH-SY5Y cells induced by MPP + , shows a dose-dependent neuroprotective effect, can effectively improve the motor dysfunction of a model animal, increase the open field movement distance, prolong the time of staying on a rotating rod, and provides a new selection for developing an anti-Parkinson's disease treatment medicine with a neuroprotective effect.

The application discloses an open field large model reasoning enhancement method based on probability process supervision. The application adopts reinforcement learning to perform multi-round iterative optimization on a strategy model: in each round, a dynamic golden thinking chain is generated based on a question and an answer as a reference, a plurality of exploratory paths are sampled, a step-level path loyalty reward is calculated through alignment to quantify logical effectiveness, a layered reward mechanism is constructed by combining a weighted process reward and answer correctness, and a final reward signal is used to update the model. The method does not require an external reward model and artificial labeling, realizes fine-grained process supervision through self-supervision, dynamically updates the supervision signal to adaptively improve the model, is highly versatile, and is suitable for various open reasoning tasks.

The invention discloses an application of aureomycin in preparation of a medicine for preventing and treating depression or anxiety behaviors. Experimental results indicate that AUN (aureusidin) can effectively increase the exploration frequency and the retention time of a model mouse on an open arm of an elevated cross labyrinth, improve the activity of the model mouse in a central area of an open field and remarkably improve depression or anxiety behaviors of the model mouse. The invention proves that the AUN has a potential effect of preventing and treating depression or anxiety behaviors, provides an experimental basis and theoretical support for developing novel medicines for treating neuropsychiatric diseases by using natural products, and has a wide application prospect.

The application belongs to the technical field of biological medicine, and specifically discloses a kind of Exosc9 The application discloses application of a gene mutation animal in construction of an anxiety model and in screening of an anti-anxiety drug. Exosc9 The gene mutation animal is a known model, but the application finds that the animal shows behavior phenotypes related to anxiety through systematic behavior experiments, including a decrease in central zone activity in an open field experiment, a decrease in open arm staying time in a high cross maze experiment, and a decrease in exploration behavior. Through behavior observation and quantitative analysis of the animal, the application can be used to study behavior characteristics of anxiety, compare phenotype differences of animals of different genotypes, genders and ages, and analyze influences of genetic and environmental factors on anxiety behavior. The application also constructs a method for screening of an anti-anxiety candidate drug and evaluation of drug efficacy based on the model, which can greatly shorten an early screening period of drug research and development, and has a wide application prospect in the field of neuropsychiatric disease research and drug research and development.

The invention provides application of Turicibacter sanguinis in preparation of a medicine for preventing and / or treating depression, and belongs to the technical field of biological medicine. Researches find that compared with depression model mice, mice treated by T. sanguinis show significant behavior improvement: sucrose consumption is increased, retention time in an interaction area in a social interaction experiment (a CD1 mouse introduction stage) is prolonged, retention time and total movement distance in a central area in an open field experiment are both increased, and the mice treated by T. sanguinis show significant behavior improvement. And meanwhile, the standing time in a forced swimming experiment is shortened. The results of the behavioral test are consistent to show that the depression-like behavior of the mouse can be obviously alleviated by the T. sanguinis intervention.

The present application belongs to the field of biological medicine and food technology, and particularly relates to application of milk-derived extracellular vesicles in improving brain health and / or improving nerve-related discomfort conditions. Compared with the prior art, the present application constructs a chronic restraint stress (CRS) induced anxiety model in adolescent mice, and uses open field test, buried bead test, tail suspension test and other behavioral experiments for functional evaluation. The results show that the milk-derived extracellular vesicles significantly improve the anxiety-like behavior of mice, indicating that the milk-derived extracellular vesicles can significantly improve the anxiety-like behavior of adolescent mice induced by chronic restraint stress (CRS) by remodeling the gut-brain axis function, and that the supplementation of milk-derived extracellular vesicles in adolescents can significantly reduce the susceptibility to anxiety in adulthood. At the same time, the present application also constructs a D-gal induced cognitive impairment model in mice, and the results show that it can reduce the production of pro-inflammatory cytokines, inhibit mucosal inflammation, thereby maintaining intestinal homeostasis and alleviating cognitive impairment.

The invention relates to the technical field of animal experiment data processing, in particular to a base line balanced random grouping and end line intelligent statistics software system for animal experiments, which comprises the following core modules: a base line data registration and overview module, a base line data analysis module and an end line analysis module, the multi-dimensional base line data correspond to eight metering indexes of six types of preset experiment plates, the six types of experiment plates comprise a sugar water test, an open field, an elevated cross, a tail suspension test (TST), forced swimming (FST) and weight, and the eight metering indexes comprise a sugar ratio (%), a total water intake (ml), a total movement distance (cm), central region time (s), arm opening time (s), FST immobile time (s), TST immobile time (s) and weight (g); according to the method, multi-index baseline equalization is realized in the grouping stage, bias is remarkably reduced, the statistical efficiency is improved, and regularized anomaly recognition is performed in the end line stage, so that subjective randomness and selective bias are reduced, and the data reliability is ensured.

The invention belongs to the technical field of medicines, and particularly relates to application of lycium barbarum glycopeptide in preparation of medicines for preventing or treating sepsis and sepsis-related encephalopathy. Mouse sepsis and sepsis-related encephalopathy models are constructed by adopting a mouse LPS (Lipopolysaccharide) method, clinical scoring, biochemical level detection and water maze and open field experiments of sepsis find that the Chinese wolfberry glycopeptide can reverse sepsis symptoms, recover mouse weight and reduce mouse death rate, and meanwhile, the Chinese wolfberry glycopeptide can be used for preventing and treating sepsis. The Chinese wolfberry glycopeptide can relieve organ injury and inflammatory factor expression of sepsis mice, relieve brain synaptic dysfunction, relieve cognitive disorder and anxiety symptoms and improve functions of sepsis-related encephalopathy. Therefore, the invention proves that the lycium barbarum glycopeptide can be effectively used for preventing and treating sepsis and sepsis-related encephalopathy, has a prospect of being developed into medicines for preventing and treating related diseases, provides more treatment choices for clinically preventing and treating sepsis and sepsis-related encephalopathy, and has important social benefits and economic values.

The utility model provides a zebra fish behavioral analysis device which comprises a box body, and a box cover is hinged to the box body. The interior of the box body is divided into a three-box social contact area, a novel water tank experiment area, a T labyrinth area, a Y labyrinth area and an open field area by partition plates; two first plate frames are arranged in the three-box social contact area, and through holes are formed in the first plate frames; the Y labyrinth area is provided with a Y-shaped channel defined by a second plate frame. The T-shaped labyrinth area is provided with a T-shaped channel defined by a third plate frame. Grid lines are drawn at the bottom of the open field area; grooves corresponding to the three-box social contact area, the novel air box experiment area, the T labyrinth area, the Y labyrinth area and the open field area are formed in the box cover, and cameras are arranged in the grooves; the camera is electrically connected with a touch control module arranged outside the box body, and animal behavior analysis software is arranged in the touch control module. The zebra fish behavioristics analysis device provided by the utility model can accurately record and analyze various behavioristics of the zebra fish, and further facilitates research and development in the fields of neurobiology, environmental toxicology and the like.

The application belongs to the technical field of biological medicine, and specifically discloses a kind of Exosc9 The application discloses application of a gene mutation animal in construction of an anxiety model and in screening of an anti-anxiety drug. Exosc9 The gene mutation animal is a known model, but the application finds that the animal shows behavior phenotypes related to anxiety through systematic behavior experiments, including a decrease in central zone activity in an open field experiment, a decrease in open arm staying time in a high cross maze experiment, and a decrease in exploration behavior. Through behavior observation and quantitative analysis of the animal, the application can be used to study behavior characteristics of anxiety, compare phenotype differences of animals of different genotypes, genders and ages, and analyze influences of genetic and environmental factors on anxiety behavior. The application also constructs a method for screening of an anti-anxiety candidate drug and evaluation of drug efficacy based on the model, which can greatly shorten an early screening period of drug research and development, and has a wide application prospect in the field of neuropsychiatric disease research and drug research and development.

The invention relates to the technical field of medicines, in particular to application of TRN in preparation of medicines for preventing or treating depression and anxiety. The TRN provided by the invention can effectively improve depression behaviors and accompanied anxiety behaviors of disease model mice (evaluated by open field experiments). In an open field experiment, TRN treatment obviously improves the total movement distance and the residence time of a central region of the mouse, and in addition, histological analysis of important organs (heart, liver, spleen, lung and kidney) of the model mouse shows that the TRN treatment has no potential toxicity. Therefore, the invention provides the application of the natural dietary flavonoids TRN in the aspect of improving depression and anxiety, and provides experimental and theoretical bases for natural products in the aspect of developing related therapeutic drugs.

The invention provides an intelligent detection method and device for leaves with plant diseases and insect pests of open-field vegetables. The method comprises the following steps: acquiring to-be-detected images of vegetable leaves in an open-field environment; inputting the to-be-detected image into the semantic segmentation model, and obtaining a pixel-level pest and disease segmentation result output by the semantic segmentation model; wherein the semantic segmentation model adopts an improved SegNeXt network structure, and comprises an encoder and a decoder; the encoder is used for receiving a to-be-detected image and processing the to-be-detected image through at least three cascaded encoding stages to generate a middle-layer feature map and a high-layer feature map; the decoder comprises a global context feature enhancement (GCFE) module and a channel efficient multi-scale attention (CEMA) module; the GCFE module is used for performing global context information extraction on the high-level feature map to generate a context enhanced feature map; and the CEMA module is used for carrying out feature fusion and attention enhancement on the context enhancement feature map and the intermediate layer feature map to obtain a pixel-level disease and insect pest segmentation result.

The invention belongs to the technical field of biological medicine and food, and particularly relates to application of milk-derived extracellular vesicles to improvement of brain health conditions and / or improvement of nerve-related discomfort conditions. Compared with the prior art, a chronic constraint stress (CRS)-induced puberty mouse anxiety model is constructed, functional evaluation is performed by behavioral experiments such as an open field experiment, a bead embedding experiment and a tail suspension experiment, and results show that the milk-derived extracellular vesicles remarkably improve mouse anxiety-like behaviors; it is shown that the milk-derived extracellular vesicles can significantly improve the chronic bound stress (CRS) induced adolescent mouse anxiety-like behavior by reshaping the intestinal-brain axis function, and the anxiety susceptibility of adolescents can be significantly reduced by supplementing the milk-derived extracellular vesicles to teenagers; meanwhile, a D-gal induced mouse cognitive impairment model is constructed, and the result shows that mucosa inflammation can be inhibited by reducing generation of proinflammatory cytokines, so that intestinal homeostasis is maintained, and cognitive impairment is relieved.

The invention discloses application of a separated USP18 gene in preparation of a medicine for treating and / or preventing Parkinson's disease, and belongs to the technical field of crossing of genetic engineering and biological pharmacy. And the nucleotide sequence of the USP18 gene is shown as SEQ ID NO. 3 or SEQ ID NO. 7. The invention provides a recombinant vector containing the gene. Through specific overexpression of USP18 in an in-vitro cell experiment and an in-vivo animal experiment, a synergistic treatment effect is generated: in an MPP + induced cell model, c-Caspase3 can be remarkably inhibited, the ratio of Bax / Bcl-2 is reduced, and neuronal apoptosis is effectively resisted; in a Parkinson's disease mouse model, loss of TH positive dopaminergic neurons in a nigra region can be specifically rescued, and the movement function in open field, pole climbing and bar rotating experiments is comprehensively improved. A brand new efficient target spot and a treatment scheme are provided for gene therapy of Parkinson's disease.

The invention belongs to the technical field of biological experiment equipment, and particularly provides an animal behavior experiment device for smoke exposure, which comprises an open field box body with an open top; the cover plate is detachably arranged at the top of the open field box body, and a closed experiment cavity is formed by the cover plate and the open field box body; the smoke generating device is connected with the open field box body through a pipeline and is used for conveying smoke to the experiment chamber; the behavior observation device and the optical genetic stimulation device are both integrally arranged on the cover plate, and the behavior observation device is matched with the smoke generation device and used for conducting behavioral observation when the animal is exposed to the smoke; and the photogenetic stimulation device is matched with the behavior observation device and is used for carrying out behavioral observation when the animals are subjected to photogenetic intervention. According to the device, environmental fluctuation, time delay and stress interference caused by animal transfer among different devices or step-by-step experiments in traditional research are overcome, and the research accuracy and efficiency are greatly improved.

The invention discloses a method for researching a mild cognitive impairment action mechanism of a cornu cervi pantotrichum polypeptide based on a Wnt / beta-catenin signal channel, relates to the technical field of mechanism analysis, and solves the problem of difficulty in realizing spanning from basic research to clinical transformation in the prior art. Randomly dividing the rats into five groups of blank control, model, positive control and VAP high / low dose, continuously performing intragastric administration on each group for 18 days, and feeding distilled water into the blank and model groups; 2 mg / kg scopolamine is injected into abdominal cavities of the model group after 18 days and 2 hours, and normal saline is injected into the blank group; the scopolamine model is used for measuring the 2min static / motion time of the rat by using an open field experiment, and the unilateral common carotid artery ligation model is used for measuring the escape latency / platform penetration times by using a water maze; the method comprises the following steps: taking blood, separating a hippocampal region, and carrying out HE dyeing, ELISA and Western blot; sPSS 21.0 analysis and single factor variance analysis are used for evaluating the difference and Plt; 0.05 or Plt; 0.01 has statistical significance.

The invention discloses an application of a medicine for relieving senile depression and a verification method of the medicine, and relates to a new application of Ro61-8048 in preparation of the medicine for relieving the senile depression, and provides a verification method based on a chronic unpredictable mild stress (CUMS) model of a 18-month-old C57BL / 6J mouse. Six behavioral indexes of sucrose preference, open field, new object recognition, Y maze, forced swimming and social interaction prove that by intraperitoneal administration of 50 mg / kg Ro61-8048, low emotion, anxiety level rise and cognitive impairment of an old depression model mouse can be remarkably reversed, the curative effect is equivalent to that of a classic antidepressant fluoxetine hydrochloride (20 mg / kg), and no obvious side effect is caused to normal old mice. The invention provides an experimental basis and a drug effect evaluation platform for taking the KMO inhibitor as a novel treatment strategy of the LLD.

The invention belongs to the technical field of microorganisms, and particularly relates to a strain of combined lactobacillus salivarius and application thereof. Through a large number of screening and verification tests, the invention invents a strain of Lactobacillus salivarius 194-07 with strong growth activity, which can inhibit DPP4 activity, at the same time, in an anxiety mouse model, the expression in mouse open field central region movement and elevated cross maze movement can be significantly improved, and the mouse neurotransmitter level can be improved; the level of inflammatory factors in hippocampal tissues in the body and the brain is inhibited, and the safety is good. The strain disclosed by the invention has good application potential in the aspects of blood glucose control, mental diseases (anxiety disorder), nerve anti-inflammation and the like.

The invention belongs to the technical field of medicines, and provides application of urolithin A acetate and a derivative thereof in preparation of a medicine for treating depression and the prepared antidepressant medicine. The application dosage is 0.01-30 mg / kg of urolithin A acetate or a derivative thereof, and the anti-depression effect can be generated within 60 minutes after administration. After the medicine is administrated for 5 days, the obvious anti-depression effect is generated in the low dosage and the high dosage. A research result shows that in a mammal mouse tail suspension experiment, the urolithin A acetate or the derivative thereof has a rapid anti-depression effect and is more effective than an anti-depression drug imipramine. According to the method, a forced swimming experiment and a mouse tail suspension experiment are adopted as drug screening experiments, and meanwhile, an open field test is carried out on the mouse, so that the autonomous activity of the mouse is detected, and interference of central excitants is avoided. The anti-depression drug has the advantages of being remarkable in anti-depression effect, quick in effect taking, small in dosage, small in side effect and the like, and is expected to become a new anti-depression drug which is quick, efficient and novel in mechanism.

The application discloses a rule and dependency grammar-based spatiotemporal correlation degree calculation method, which comprises the following steps: obtaining an open field text sequence, and preprocessing the text sequence to obtain a preprocessed text sequence; performing syntactic analysis on the preprocessed text sequence, formulating a rule matching table, and matching the syntactic analysis result according to the rule matching table to obtain candidate triplets and spatiotemporal information; calculating a spatiotemporal correlation degree value according to a dependency path of a relation predicate in the candidate triplets and the spatiotemporal information, a dependency distance of a head entity and the spatiotemporal information, a spatiotemporal feature of head and tail entity types, and a spatiotemporal semantic feature of the relation predicate; defining two threshold values, comparing the spatiotemporal correlation degree value with the two threshold values, determining a classification result of the spatiotemporal correlation degree, and generating a knowledge expression model. The application accurately calculates spatiotemporal correlation by comprehensively considering the context and spatiotemporal information of the text, thereby obtaining a spatiotemporal knowledge expression model and more accurately conveying the semantics of the text.

The invention belongs to the technical field of application of natural medicines, and particularly discloses application of SSK-9-1 in preparation of medicines for preventing or treating depression and anxiety. Animal behavioral experiments prove that SSK-9-1 shows a positive improvement effect on depression and anxiety behaviors of a depression model mouse, and the exploration behavior and interest level of the mouse in a new environment can be improved. Specifically, in an open field experiment, SSK-9-1 can significantly prolong the residence time of a depression mouse in a central area and increase the number of times of entering the central area; in an elevated cross maze experiment, the SSK-9-1 can obviously increase the number of times that a depression mouse enters an open arm and the staying time of the depression mouse in a target area. SSK-9-1 provides a new thought and basis for developing natural medicines to prevent or treat depression and anxiety, and has a good application prospect.

This invention discloses a Z-shaped maze device and experimental method for directional physical field exposure experiments, relating to the field of biomedical engineering technology. The device includes a box containing a maze area and an open field area. Multiple parallel partitions are arranged within the maze area, alternately connected to the inner walls of opposite sides of the box, with gaps maintained between the ends of the partitions and adjacent inner walls. These gaps, along with the space between adjacent partitions, constitute the passageway for the experimental animal. The width of the passageway is designed according to the principle of "allowing only unidirectional passage for the experimental animal, preventing it from turning around within the passageway." The end of the passageway connects to the open field area. This invention achieves, for the first time, precise and coordinated control of the animal's position and directional physical field parameters throughout the entire process in an unrestrained state. It can comprehensively cover all parameter research scenarios for all types of directional physical fields. It has strong universality and flexibility, enabling simultaneous acquisition of multi-dimensional behavioral indicators. The experimental process is standardized.

The invention discloses a credible reasoning and question answering method and device based on cooperation of a large model and a knowledge graph, and belongs to the technical field of artificial intelligence and natural language processing. Keywords are extracted through semantic guidance and linked to a graph entity, and an initial entity is determined; path exploration is carried out based on a decision-evaluation cooperation mechanism, a path is expanded in combination with hard constraint and semantic soft guidance, and dynamic pruning is carried out according to an instant reward difference value and an endurance threshold value; and finally outputting an answer and a traceable reasoning path. The problems that entity recognition depends on rules, the reasoning process is black-box-shaped and the interpretability is poor in open domain questions and answers are solved, the semantic understanding generalization ability and the reasoning process controllability and credibility are considered, the questions and answers accuracy, reasoning efficiency and result credibility are improved, and the comprehensive performance is better than that of a traditional method based on rules, heuristic or pure neural networks.

The invention discloses a method for regulating a hippocampus immune balance mechanism of a stress depression mouse through electroshock, and relates to the field of nerves and spirit. Comprising the following steps: constructing a chronic unpredictable mild stress CUMS depression-like mouse model, and performing intervention by adopting electroshock pricking ECS; depression-like behaviors are evaluated through behavioral tests, including a sweet water preference experiment, an open field experiment and a tail suspension experiment; the method comprises the following steps: collecting hippocampus tissues of a mouse, and detecting the level of inflammatory factors and the polarization state of microglial cells by using ELISA, Western blot and an immunofluorescence technology; and evaluating the activation of the microglial cells by combining PET-CT scanning with a [< 18 > F] DPA-714 tracer agent. By integrating clinical, animal and cell experiments, the invention reveals that ECT recovers the balance of microglial cells M1 / M2 through a Chuk / NF-kB signal channel, thereby relieving the depressive symptom.

The utility model discloses a multifunctional toolbox suitable for the field, which comprises a box body, a plurality of partition areas arranged in the box body and used for storing tools in a classified manner; the separation areas and tools comprise an electronic portable scale separation area, an electronic portable scale, a large flat shear separation area, a large flat shear, an isolation line separation area, an isolation line, a steel tape separation area, a steel tape, an electric branch shear separation area, an electric branch shear and accessories of the electric branch shear. The utility model relates to an electric mower isolation area, an electric mower and accessories thereof, a measuring drill isolation area, a measuring drill, a labor protection isolation area, labor protection gloves and labor protection kneecaps. The tool box is not only suitable for botany teaching and scientific research activities, but also widely suitable for various scenes such as field investigation and ecological investigation, and provides comprehensive tool support for professionals and students.