47 results about "Open field" patented technology

The invention discloses application of an Hspa5 inhibitor in preparation of a medicine for preventing or treating anxiety disorder. An anxiety mouse model is constructed through chronic constraint stress (CRS), and in combination with medial amygdala kernel (MeA) transcriptome sequencing and qPCR verification, it is found that the endoplasmic reticulum molecular chaperone Hspa5 is remarkably up-regulated in the anxiety state. Furthermore, an Hspa5 specific inhibitor HA15 is locally injected into a MeA brain region, so that the anxiety-like behavior induced by the CRS is remarkably improved, and the exploration time of an open field experiment central region, the exploration time of an open arm of an elevated cross labyrinth and the exploration time of a bright box of a bright-dark box experiment are prolonged. The invention discloses the function of Hspa5 as a novel anti-anxiety target for the first time, and provides a direct experimental basis and a transformation direction for developing a novel anti-anxiety drug which is non-monoamine and targets an endoplasmic reticulum homeostasis.

The invention discloses an odorant for relieving fear emotion. The active ingredients of the odorant are linalyl acetate and 1, 8-cineole. According to the present invention, it is found that the mice can smells linalyl acetate and 1, 8-cineole every day so as to effectively improve the behavioral abnormality caused by fear emotion, the effect is evaluated through scene fear, open field and elevated cross maze experiments, and the experimental result shows that the combined use of the two odorants can significantly improve the fear emotion of the mice. The discovery provides a new thought for research and development of products related to prevention and treatment of fear emotion.

The invention relates to the technical field of mining, in particular to a mining and cutting parameter intelligent optimization method and system based on a small-section open-stope method. The method comprises the following steps: acquiring historical mining case data, and training an index prediction model by using the historical mining case data; geological data of a to-be-mined unit are obtained, and geological feature data are extracted from the geological data; based on the geological feature data, the index prediction model uses a multi-objective optimization algorithm to calculate an optimal mining and cutting parameter scheme set; and selecting an optimal mining and cutting parameter in the optimal mining and cutting parameter scheme set based on the index prediction model and the geological feature data. The problem that in the prior art, mining and cutting parameter calculation based on a small-segment open-field method is not accurate enough is solved, an index prediction model is trained through historical mining case data, a scheme set is generated through multi-target optimization in combination with geological features of a to-be-mined unit, and then adaptive optimal mining and cutting parameters are screened, so that the mining and cutting parameter calculation accuracy is improved. And the accuracy of dynamic calculation of the mining and cutting parameters is greatly improved.

The invention discloses an application of simvastatin in preparation of a medicine for treating post-traumatic stress disorder. According to the invention, a post-traumatic stress disorder model is firstly constructed, and a result shows that in post-traumatic stress disorder model animals, obvious core behavior characteristics such as fear memory overconsolidation and anxiety behavior enhancement are shown. When simvastatin is used for intervention treatment, the fear memory fading ability of the model animal is obviously improved, and the anxiety behavior is obviously relieved; behavior experiment comprehensive evaluation shows that the stiffness time of animals in a simvastatin treatment group is remarkably shortened, the standing time and distance proportion of an open arm of an elevated cross maze are remarkably increased, the total movement distance of an open field experiment is remarkably increased, and simvastatin can effectively relieve fear memory disorder and anxiety behaviors of post-traumatic stress disorder model animals.

The application relates to the technical field of new uses of medicines, and particularly discloses application of an N-phenylpiperazine derivative in preparation of a medicine for treating Parkinson's disease, wherein the N-phenylpiperazine derivative is HS. + The N-phenylpiperazine derivative HS provided by the application can significantly improve the survival rate of SH-SY5Y cells induced by MPP + , shows a dose-dependent neuroprotective effect, can effectively improve the motor dysfunction of a model animal, increase the open field movement distance, prolong the time of staying on a rotating rod, and provides a new selection for developing an anti-Parkinson's disease treatment medicine with a neuroprotective effect.

The application discloses an open field large model reasoning enhancement method based on probability process supervision. The application adopts reinforcement learning to perform multi-round iterative optimization on a strategy model: in each round, a dynamic golden thinking chain is generated based on a question and an answer as a reference, a plurality of exploratory paths are sampled, a step-level path loyalty reward is calculated through alignment to quantify logical effectiveness, a layered reward mechanism is constructed by combining a weighted process reward and answer correctness, and a final reward signal is used to update the model. The method does not require an external reward model and artificial labeling, realizes fine-grained process supervision through self-supervision, dynamically updates the supervision signal to adaptively improve the model, is highly versatile, and is suitable for various open reasoning tasks.

The invention discloses application of bitter gourd exosomes in regulating intestinal flora. The method comprises the following steps: establishing an Alzheimer's disease mouse model, injecting the extracted balsam pear exosome into the mouse body in a gavage manner to serve as an experimental group drug, taking normal saline as a model negative control group, detecting the change of the intestinal flora of the AD mouse by applying a 16S amplicon sequencing principle, and detecting the change of the intestinal flora metabolite of the AD mouse by applying an LS-MS technology. An open field experiment, a nesting experiment and a water maze experiment are adopted to verify the treatment effect of the balsam pear exosome on AD mouse intestinal flora and metabolic level changes thereof. Results prove that the bitter gourd exosome extracted by the invention can effectively improve the intestinal flora micro-ecology of AD mice; the compound can be used for preparing a microecological preparation for regulating intestinal flora of neurodegenerative diseases such as Alzheimer's disease, Parkinson's disease, Huntington's disease, amyotrophic lateral sclerosis, different types of spinal cerebellar ataxia and the like and diseases such as cerebral apoplexy, cerebral injury, epilepsy, tumor and the like.

The application provides a spatial memory task device and a paradigm method based on a self-center and heterocentric navigation strategy. The device comprises an open field, a reward plate and a rest box. The open field is formed by a bottom plate and four side plates and has an open top end. The rest box opening is formed in the middle of the four side plates. The rest box door is installed at the rest box opening. The rest box is installed outside the side plate and is arranged correspondingly to the rest box opening. The reward wells are uniformly distributed on the reward plate. The reward wells are used for containing water. The reward plate is placed on the bottom plate. The experimental process comprises water limiting of the rats before the experiment and the experimental stage, i.e. a free exploration stage and a spatial memory task stage. The beneficial effects of the application are as follows: the paradigm method is used to measure the navigation strategy priority of the rats when the rats navigate, and to evaluate the strategy damage of the rats with Alzheimer's disease and how the rats with Alzheimer's disease navigate after the damage.

The invention discloses an application of aureomycin in preparation of a medicine for preventing and treating depression or anxiety behaviors. Experimental results indicate that AUN (aureusidin) can effectively increase the exploration frequency and the retention time of a model mouse on an open arm of an elevated cross labyrinth, improve the activity of the model mouse in a central area of an open field and remarkably improve depression or anxiety behaviors of the model mouse. The invention proves that the AUN has a potential effect of preventing and treating depression or anxiety behaviors, provides an experimental basis and theoretical support for developing novel medicines for treating neuropsychiatric diseases by using natural products, and has a wide application prospect.

The application belongs to the technical field of biological medicine, and specifically discloses a kind of Exosc9 The application discloses application of a gene mutation animal in construction of an anxiety model and in screening of an anti-anxiety drug. Exosc9 The gene mutation animal is a known model, but the application finds that the animal shows behavior phenotypes related to anxiety through systematic behavior experiments, including a decrease in central zone activity in an open field experiment, a decrease in open arm staying time in a high cross maze experiment, and a decrease in exploration behavior. Through behavior observation and quantitative analysis of the animal, the application can be used to study behavior characteristics of anxiety, compare phenotype differences of animals of different genotypes, genders and ages, and analyze influences of genetic and environmental factors on anxiety behavior. The application also constructs a method for screening of an anti-anxiety candidate drug and evaluation of drug efficacy based on the model, which can greatly shorten an early screening period of drug research and development, and has a wide application prospect in the field of neuropsychiatric disease research and drug research and development.

The utility model discloses a mouse open field experiment device convenient to clean. The mouse open field experiment device comprises an open field platform, a supporting mechanism arranged on the open field platform, a lifting mechanism which is arranged on the outer side of the supporting mechanism and can move up and down, and a guide mechanism arranged on the open field platform. The guide mechanism comprises two guide plates arranged on the front side and the rear side of the open field platform respectively and two guide grooves formed in the opposite faces of the two guide plates respectively. Meanwhile, cleaning mechanisms capable of moving left and right along the surface of the open field platform are arranged on the two guide grooves; the open field platform has the advantages that the open field platform can be quickly switched to be opened / closed through matching of the supporting mechanism and the lifting mechanism, the open field platform can be exposed when the open field platform is in an upward moving and opening state of the test area, excrement and other impurities on the surface of the open field platform can be conveniently and quickly cleaned by the cleaning mechanism, and meanwhile experimental mice can be conveniently placed in the open field platform; and when the test area moves downwards and is closed, a mouse test open field is reconstructed.

The invention provides application of Turicibacter sanguinis in preparation of a medicine for preventing and / or treating depression, and belongs to the technical field of biological medicine. Researches find that compared with depression model mice, mice treated by T. sanguinis show significant behavior improvement: sucrose consumption is increased, retention time in an interaction area in a social interaction experiment (a CD1 mouse introduction stage) is prolonged, retention time and total movement distance in a central area in an open field experiment are both increased, and the mice treated by T. sanguinis show significant behavior improvement. And meanwhile, the standing time in a forced swimming experiment is shortened. The results of the behavioral test are consistent to show that the depression-like behavior of the mouse can be obviously alleviated by the T. sanguinis intervention.

The present application belongs to the field of biological medicine and food technology, and particularly relates to application of milk-derived extracellular vesicles in improving brain health and / or improving nerve-related discomfort conditions. Compared with the prior art, the present application constructs a chronic restraint stress (CRS) induced anxiety model in adolescent mice, and uses open field test, buried bead test, tail suspension test and other behavioral experiments for functional evaluation. The results show that the milk-derived extracellular vesicles significantly improve the anxiety-like behavior of mice, indicating that the milk-derived extracellular vesicles can significantly improve the anxiety-like behavior of adolescent mice induced by chronic restraint stress (CRS) by remodeling the gut-brain axis function, and that the supplementation of milk-derived extracellular vesicles in adolescents can significantly reduce the susceptibility to anxiety in adulthood. At the same time, the present application also constructs a D-gal induced cognitive impairment model in mice, and the results show that it can reduce the production of pro-inflammatory cytokines, inhibit mucosal inflammation, thereby maintaining intestinal homeostasis and alleviating cognitive impairment.

The invention relates to the technical field of animal experiment data processing, in particular to a base line balanced random grouping and end line intelligent statistics software system for animal experiments, which comprises the following core modules: a base line data registration and overview module, a base line data analysis module and an end line analysis module, the multi-dimensional base line data correspond to eight metering indexes of six types of preset experiment plates, the six types of experiment plates comprise a sugar water test, an open field, an elevated cross, a tail suspension test (TST), forced swimming (FST) and weight, and the eight metering indexes comprise a sugar ratio (%), a total water intake (ml), a total movement distance (cm), central region time (s), arm opening time (s), FST immobile time (s), TST immobile time (s) and weight (g); according to the method, multi-index baseline equalization is realized in the grouping stage, bias is remarkably reduced, the statistical efficiency is improved, and regularized anomaly recognition is performed in the end line stage, so that subjective randomness and selective bias are reduced, and the data reliability is ensured.

The invention belongs to the technical field of animal model establishment, and discloses an establishment method and application of a chronic oral ferric citrate induced Parkinson mouse model, C57BL / 6 mice are fed with mouse maintenance food (high-iron feed) added with 2.25% ferric citrate for 22 weeks, and then a 5% ferric citrate solution is used for intragastric administration for 4 weeks. Finally, behavioral evaluation is carried out through behavioral tests such as an open field test, a rotating rod test, a pole climbing test, a holding power test and a Y labyrinth test, and the tests such as the rotating rod test, the pole climbing test and the holding power test show that the PD model mouse has remarkable motor function impairment such as reduction of motor coordination, muscular tension and balance force; open field tests and Y maze show that learning and memory functions of mice are impaired, and cognitive impairments such as anxiety mood occur. Clinical chronic disease conditions of Parkinson's disease are simulated, and the chronic model is more suitable for monitoring early changes and screening early diagnosis indexes of Parkinson's disease; the method has the characteristics that the behavioral disorder can be quantified, and DA energy neuron degeneration loss characteristic lesion is realized.

The invention belongs to the technical field of medicines, and particularly relates to application of lycium barbarum glycopeptide in preparation of medicines for preventing or treating sepsis and sepsis-related encephalopathy. Mouse sepsis and sepsis-related encephalopathy models are constructed by adopting a mouse LPS (Lipopolysaccharide) method, clinical scoring, biochemical level detection and water maze and open field experiments of sepsis find that the Chinese wolfberry glycopeptide can reverse sepsis symptoms, recover mouse weight and reduce mouse death rate, and meanwhile, the Chinese wolfberry glycopeptide can be used for preventing and treating sepsis. The Chinese wolfberry glycopeptide can relieve organ injury and inflammatory factor expression of sepsis mice, relieve brain synaptic dysfunction, relieve cognitive disorder and anxiety symptoms and improve functions of sepsis-related encephalopathy. Therefore, the invention proves that the lycium barbarum glycopeptide can be effectively used for preventing and treating sepsis and sepsis-related encephalopathy, has a prospect of being developed into medicines for preventing and treating related diseases, provides more treatment choices for clinically preventing and treating sepsis and sepsis-related encephalopathy, and has important social benefits and economic values.

The invention provides application of Allo-aca in preparation of a medicine for improving liver injury and concurrent neurological symptoms, and belongs to the technical field of medicines. The Allo-aca is a leptin receptor antagonist, and the core action mechanism of the Allo-aca is that the Allo-aca is competitively combined with a leptin receptor to block a leptin signal transduction pathway, so that the leptin-dependent pathological process is intervened. Experimental verification shows that the Allo-aca can significantly reduce the levels of glutamic-pyruvic transaminase and glutamic oxalacetic transaminase in mouse serum induced by chronic stress + AFB1, relieve liver cell edema balloon-like degeneration and inflammatory cell infiltration, and realize liver protection; meanwhile, the incubation period of a mouse dark avoidance experiment can be prolonged, the success frequency of a shuttle experiment is increased, the center staying time of an open field and the staying time of an open arm of an elevated cross maze are prolonged, and cognitive impairment and anxiety mood are effectively improved. The invention provides a new treatment scheme for the chronic stress induced AFB1 hepatotoxicity aggravation effect.

The invention belongs to the technical field of medicines, and particularly provides a novel application of sodium butyrate in preparation of a product for treating DYT25 dysmnesia. DYT25 muscular dystonia mouse (Gal + / -) experiment evaluation proves that the sodium butyrate can obviously increase the falling time, the learning rate and the initial coordination in an accelerated bar rotating experiment; the descending time and the turning time in a vertical rod experiment are remarkably shortened, and the passing time and the sliding frequency in a horizontal rod experiment are shortened; and the movement distance and the average speed in an open field experiment are increased. Therefore, the sodium butyrate provided by the invention can be applied to preparation of products for treating DYT25 dysmnesia.

The utility model provides a zebra fish behavioral analysis device which comprises a box body, and a box cover is hinged to the box body. The interior of the box body is divided into a three-box social contact area, a novel water tank experiment area, a T labyrinth area, a Y labyrinth area and an open field area by partition plates; two first plate frames are arranged in the three-box social contact area, and through holes are formed in the first plate frames; the Y labyrinth area is provided with a Y-shaped channel defined by a second plate frame. The T-shaped labyrinth area is provided with a T-shaped channel defined by a third plate frame. Grid lines are drawn at the bottom of the open field area; grooves corresponding to the three-box social contact area, the novel air box experiment area, the T labyrinth area, the Y labyrinth area and the open field area are formed in the box cover, and cameras are arranged in the grooves; the camera is electrically connected with a touch control module arranged outside the box body, and animal behavior analysis software is arranged in the touch control module. The zebra fish behavioristics analysis device provided by the utility model can accurately record and analyze various behavioristics of the zebra fish, and further facilitates research and development in the fields of neurobiology, environmental toxicology and the like.

The application belongs to the technical field of medicines, and specifically discloses application of CL in preparation of medicines for preventing or treating depression and anxiety. Cycloneolitsol (CL) is a triterpene compound of the cyclalite class which is separated and extracted from the leaves of Podocarpus nryophyllus. Animal experiments prove that CL can effectively improve the depressive behavior of aging model mice and the anxiety behavior (open field and elevated plus maze) often accompanied by depression, can increase the staying time of mice in the center area in the open field experiment, and significantly increase the number of times of entering the open arm and the staying time in the target area open arm of the anxiety mice in the elevated plus maze experiment; and CL has no toxicity to the organs such as heart, liver, spleen, lung and kidney of mice, and the weight of mice does not decrease obviously, and the safety is relatively high. CL can be used for preparing medicines for preventing or treating depression and anxiety, and has a wide application prospect.

The application belongs to the technical field of biological medicine, and specifically discloses a kind of Exosc9 The application discloses application of a gene mutation animal in construction of an anxiety model and in screening of an anti-anxiety drug. Exosc9 The gene mutation animal is a known model, but the application finds that the animal shows behavior phenotypes related to anxiety through systematic behavior experiments, including a decrease in central zone activity in an open field experiment, a decrease in open arm staying time in a high cross maze experiment, and a decrease in exploration behavior. Through behavior observation and quantitative analysis of the animal, the application can be used to study behavior characteristics of anxiety, compare phenotype differences of animals of different genotypes, genders and ages, and analyze influences of genetic and environmental factors on anxiety behavior. The application also constructs a method for screening of an anti-anxiety candidate drug and evaluation of drug efficacy based on the model, which can greatly shorten an early screening period of drug research and development, and has a wide application prospect in the field of neuropsychiatric disease research and drug research and development.

The invention relates to the technical field of traditional Chinese medicines, relates to a new application of a traditional Chinese medicine composition, and in particular relates to an application of the traditional Chinese medicine composition in preparing a medicine for preventing and / or treating depression. The traditional Chinese medicine composition is prepared from cassia twig, white peony root, honey-fried licorice root, ginger, jujube, ginseng, Chinese angelica, salted morinda officinalis and dwarf lilyturf tuber. The traditional Chinese medicine composition provided by the invention can obviously increase the body weight and social interaction index of a social contusion depression model mouse and reduce the corticosterone content in serum; and the total movement distance and the central region retention time of an open field experiment in a chronic mild stress rat depression model are obviously increased, the content of 5-HT in plasma is increased, and a good treatment effect on depression is achieved.

The invention discloses application of lupinone (Lup) in preparation of drugs for preventing or treating depression and anxiety, and belongs to the technical field of application of natural drugs. The results of an open field experiment and an elevated cross maze experiment show that the Lup can effectively improve the depression behavior and the accompanying anxiety behavior of a disease model mouse. Meanwhile, a differential gene protein interaction network is obtained by analyzing a transcriptome of an experimental mouse, and a result shows that gene expressions of Acta2, Fos, Tagln, Vwf, Ccl2 and the like have significant differences and can possibly play key biological functions under experimental conditions, so that valuable clues and directions are provided for subsequent in-depth research, and the application prospect is broad. And further analysis of related biological mechanisms and pathological processes is facilitated. The result shows that Lup has potential treatment value in the aspect of improving depression and anxiety symptoms, and an experimental basis is provided for developing related treatment drugs.

The invention relates to the technical field of medicines, in particular to application of TRN in preparation of medicines for preventing or treating depression and anxiety. The TRN provided by the invention can effectively improve depression behaviors and accompanied anxiety behaviors of disease model mice (evaluated by open field experiments). In an open field experiment, TRN treatment obviously improves the total movement distance and the residence time of a central region of the mouse, and in addition, histological analysis of important organs (heart, liver, spleen, lung and kidney) of the model mouse shows that the TRN treatment has no potential toxicity. Therefore, the invention provides the application of the natural dietary flavonoids TRN in the aspect of improving depression and anxiety, and provides experimental and theoretical bases for natural products in the aspect of developing related therapeutic drugs.

The invention provides an intelligent detection method and device for leaves with plant diseases and insect pests of open-field vegetables. The method comprises the following steps: acquiring to-be-detected images of vegetable leaves in an open-field environment; inputting the to-be-detected image into the semantic segmentation model, and obtaining a pixel-level pest and disease segmentation result output by the semantic segmentation model; wherein the semantic segmentation model adopts an improved SegNeXt network structure, and comprises an encoder and a decoder; the encoder is used for receiving a to-be-detected image and processing the to-be-detected image through at least three cascaded encoding stages to generate a middle-layer feature map and a high-layer feature map; the decoder comprises a global context feature enhancement (GCFE) module and a channel efficient multi-scale attention (CEMA) module; the GCFE module is used for performing global context information extraction on the high-level feature map to generate a context enhanced feature map; and the CEMA module is used for carrying out feature fusion and attention enhancement on the context enhancement feature map and the intermediate layer feature map to obtain a pixel-level disease and insect pest segmentation result.

The present invention provides a method for three-dimensional positioning of radioactive materials in an open field, comprising: step S1, formulating a flight plan; step S2, collecting data; step S3, collecting environmental information; step S4, transmitting data back; step S5, filtering and preprocessing the data; step S6, inverting and solving for α; step S7, inverting and solving for α1; step S8, determining azimuth and elevation angles; and step S9, determining the coordinates of the radioactive material in a spherical coordinate system. The present invention also provides a three-dimensional positioning system for radioactive materials in an open field.

The invention belongs to the technical field of biological medicine and food, and particularly relates to application of milk-derived extracellular vesicles to improvement of brain health conditions and / or improvement of nerve-related discomfort conditions. Compared with the prior art, a chronic constraint stress (CRS)-induced puberty mouse anxiety model is constructed, functional evaluation is performed by behavioral experiments such as an open field experiment, a bead embedding experiment and a tail suspension experiment, and results show that the milk-derived extracellular vesicles remarkably improve mouse anxiety-like behaviors; it is shown that the milk-derived extracellular vesicles can significantly improve the chronic bound stress (CRS) induced adolescent mouse anxiety-like behavior by reshaping the intestinal-brain axis function, and the anxiety susceptibility of adolescents can be significantly reduced by supplementing the milk-derived extracellular vesicles to teenagers; meanwhile, a D-gal induced mouse cognitive impairment model is constructed, and the result shows that mucosa inflammation can be inhibited by reducing generation of proinflammatory cytokines, so that intestinal homeostasis is maintained, and cognitive impairment is relieved.

The invention discloses a traditional Chinese medicine composition for preventing and / or treating anxiety and / or insomnia. The traditional Chinese medicine composition is prepared from rhizoma polygonati, fructus crataegi, fructus schizandrae, radix polygalae, flos rosae rugosae, poria cocos and orange peel. Wherein the mass ratio or the mass concentration ratio of the rhizoma polygonati to the hawthorn to the schisandra chinensis to the polygala tenuifolia to the rose to the poria cocos to the orange peel is (1.5-5) to (0.5-5) to (0.5-5) to (0.5-5) to (1.5-5) to (0.5-5) to (0.5-5). The traditional Chinese medicine composition is prepared from medicinal and edible traditional Chinese medicines including rhizoma polygonati, hawthorn, schisandra chinensis, polygala tenuifolia, rose, poria cocos and orange peel according to a certain mass ratio, and is screened by applying uniform design in open field experiments and threshold dose pentobarbital sodium mouse sleep experiments respectively. According to the composition, the central area retention rate of open field experiment mice is remarkably increased; the traditional Chinese medicine composition has a remarkable effect of preventing and treating anxiety and / or insomnia, and the effect of the traditional Chinese medicine composition is superior to that of traditional Chinese medicine compositions in other proportions.

The invention discloses application of a separated USP18 gene in preparation of a medicine for treating and / or preventing Parkinson's disease, and belongs to the technical field of crossing of genetic engineering and biological pharmacy. And the nucleotide sequence of the USP18 gene is shown as SEQ ID NO. 3 or SEQ ID NO. 7. The invention provides a recombinant vector containing the gene. Through specific overexpression of USP18 in an in-vitro cell experiment and an in-vivo animal experiment, a synergistic treatment effect is generated: in an MPP + induced cell model, c-Caspase3 can be remarkably inhibited, the ratio of Bax / Bcl-2 is reduced, and neuronal apoptosis is effectively resisted; in a Parkinson's disease mouse model, loss of TH positive dopaminergic neurons in a nigra region can be specifically rescued, and the movement function in open field, pole climbing and bar rotating experiments is comprehensively improved. A brand new efficient target spot and a treatment scheme are provided for gene therapy of Parkinson's disease.

The application discloses Bifidobacterium breve and application thereof in relieving Alzheimer's disease and belongs to the technical field of probiotics. 18 Bifidobacterium strains are separated from the feces of children aged 0-3 years old, and are identified as Bifidobacterium longum, Bifidobacterium breve and Bifidobacterium animalis through analysis and identification. A Bifidobacterium breve LE4 strain with potential ability of relieving Alzheimer's disease is screened out through in-vitro antioxidant and LPS clearance capacity screening. Through behavior experiment (nest building experiment, open field experiment, water maze experiment), histopathological analysis, molecular biology detection and other methods, the LE4 strain is comprehensively evaluated to have the effects of improving cognitive function, reducing Aβ deposition and tau protein phosphorylation, reducing neural inflammation, enhancing synaptic plasticity, repairing intestinal barrier, regulating intestinal flora and metabolites and other multi-target point intervention AD, and provides a new strategy for promoting AD microecological treatment.