81 results about "Open field" patented technology

The invention provides a mouse hangover degree accurate detection mathematical analysis model construction method and application. Specifically, the invention provides a method for constructing a hangover degree accurate detection mathematical analysis model, and the method comprises the following steps: carrying out wine gavage and ethanol gavage on a mouse, carrying out an open field experiment and a rotating bar experiment, and detecting the concentration of ethanol in the blood of the mouse, so as to obtain each index numerical value, thereby obtaining the hangover degree of the mouse. The method disclosed by the invention is simple and convenient to operate and high in credibility and sensitivity, and has important significance in actual production and application of the wine industry.

The invention provides an application of dihydroquercetin in preparation of an antidepressant drug. The dihydroquercetin is found to be capable of effectively improving the depressive symptom induced by IBD for the first time, and compared with a traditional treatment method, the medicine (TAX) shows a more excellent effect in the aspect of improving the depressive symptom of IBD mice. Through behavioral tests, such as a forced swimming test and an open-field test, the TAX obviously reduces the immobility time and the floating time of a mouse under a medium dose (50mg / kg), and shows an obvious anti-depression effect. Therefore, the dihydroquercetin has a great application prospect in preparation of the antidepressant drugs.

The invention provides a composition of probiotics and application of the composition in treatment of insomnia. More specifically, sleep promoting peptide CSM-3 obtained through specific screening is further added into the composition, and the sleep promoting peptide can improve the activity rule of rats suffering from insomnia in an open field and can promote synthesis of 5-HIAA so as to improve sleep. After the polypeptide and probiotics are combined for use, the effect of obviously promoting the sleep increase of mice can be achieved, and the application prospect is good.

The invention discloses a free exploration normal form device and method for different spatial dimensions based on rich information, and the device comprises a one-dimensional annular one-way free exploration normal form device, a two-dimensional open field free exploration normal form device, and a three-dimensional behavior box free exploration normal form device. By using the free exploration normal form device with different spatial dimensions, not only can spatial information characterization of rat hippocampus position cells for different spatial dimensions be explored, but also whether dynamic change of long-term simulation microgravity for rat spatial information coding capability has a certain time-varying rule can be explored. Conditions are created for subsequently ascertaining and simulating internal neural mechanisms of influence of microgravity factors on spatial memory and the like.

The invention discloses a dendrobium huoshanense extract and application of the dendrobium huoshanense extract in preparation of a medicine for preventing and / or treating depression, and belongs to the technical field of biological medicine. The dendrobium huoshanense extract is obtained through reflux extraction by using an alcohol-water mixed solution, and the volume concentration of alcohol in the alcohol-water mixed solution is 50%-90%. The reflux extraction conditions are as follows: the temperature is 70-90 DEG C, and the time is 1-4 hours. According to behavioral data analysis of mouse sweet water preference, tail suspension test, forced swimming test, open field test, elevated cross labyrinth test and the like, the dendrobium huoshanense extract has a remarkable anti-depression effect, has a good effect of preventing and / or treating depression, and has a good application prospect in prevention and / or treatment of depression.

The invention belongs to the field of neurobiology and drug research and development, and discloses application of NR3A as a protein target in screening or preparing drugs for preventing and / or treating depression-like behaviors. According to the invention, the expression of NR3A in hippocampus is enhanced by stereotactic injection of adeno-associated virus containing Grin3a gene. A chronic constraint stress (CRS) is adopted to induce a depressive mouse, and a sucrose preference test (SPT), a forced swimming test (FST) and an open field test (OFT) are adopted for identification. The expression of proteins such as NR3A, calpain 2 (Calpain2), neuron nitric oxide synthase (nNOS), 3-nitrotyrosine (3-NT), an NLRP3 inflammation complex and the like is detected by WesternBlot; and performing DHE staining to detect reactive oxygen species (ROS). By enhancing hippocampus NR3A expression, depression-like behaviors induced by CRS can be improved, signal cascade activated by calcium is inhibited, ROS generation and 3-NT expression are reduced, and neuroinflammation driven by NLRP3 inflammasomes is inhibited. The NR3A participates in depression-like behaviors induced by CRS, and the mechanism of the NR3A is related to calcium homeostasis regulation, ROS generation and inflammatory response driven by NLRP3 inflammasomes.

The invention discloses application of an Hspa5 inhibitor in preparation of a medicine for preventing or treating anxiety disorder. An anxiety mouse model is constructed through chronic constraint stress (CRS), and in combination with medial amygdala kernel (MeA) transcriptome sequencing and qPCR verification, it is found that the endoplasmic reticulum molecular chaperone Hspa5 is remarkably up-regulated in the anxiety state. Furthermore, an Hspa5 specific inhibitor HA15 is locally injected into a MeA brain region, so that the anxiety-like behavior induced by the CRS is remarkably improved, and the exploration time of an open field experiment central region, the exploration time of an open arm of an elevated cross labyrinth and the exploration time of a bright box of a bright-dark box experiment are prolonged. The invention discloses the function of Hspa5 as a novel anti-anxiety target for the first time, and provides a direct experimental basis and a transformation direction for developing a novel anti-anxiety drug which is non-monoamine and targets an endoplasmic reticulum homeostasis.

The invention relates to a construction method and application of a loneliness mouse model, and the method comprises the steps: placing an experimental mouse in an isolation region in a transparent acrylic isolation cover through an environment in which physical isolation and social deprivation stimulation coexist, forming a visually accessible but physically isolated social environment with five normal living mice, and continuously inducing chronic loneliness for 4 weeks. The effectiveness of the model is verified through a multi-dimensional evaluation system, including behavioristics (open field, elevated maze and three-box experiment), neuroendocrine (serum noradrenaline and angiotensin II), immune inflammatory factors (IL-1beta, IL-6, TNF-alpha and CCL5) and nerve activation (blue spot, almond nucleus, lateral nucleus septum, medial prefrontal cortex and center joint dorsal nucleus C-Fos staining) indexes. According to the intervention method, dynamic environment abundance and social stimulation are adopted, running wheels are arranged, a modular abundance device is arranged, and a social environment of new mice is introduced, so that pathological phenotypes caused by loneliness are reversed. And a systematic experiment tool is provided for exploring association of aloneness and diseases.

The invention discloses an odorant for relieving fear emotion. The active ingredients of the odorant are linalyl acetate and 1, 8-cineole. According to the present invention, it is found that the mice can smells linalyl acetate and 1, 8-cineole every day so as to effectively improve the behavioral abnormality caused by fear emotion, the effect is evaluated through scene fear, open field and elevated cross maze experiments, and the experimental result shows that the combined use of the two odorants can significantly improve the fear emotion of the mice. The discovery provides a new thought for research and development of products related to prevention and treatment of fear emotion.

The invention relates to the technical field of mining, in particular to a mining and cutting parameter intelligent optimization method and system based on a small-section open-stope method. The method comprises the following steps: acquiring historical mining case data, and training an index prediction model by using the historical mining case data; geological data of a to-be-mined unit are obtained, and geological feature data are extracted from the geological data; based on the geological feature data, the index prediction model uses a multi-objective optimization algorithm to calculate an optimal mining and cutting parameter scheme set; and selecting an optimal mining and cutting parameter in the optimal mining and cutting parameter scheme set based on the index prediction model and the geological feature data. The problem that in the prior art, mining and cutting parameter calculation based on a small-segment open-field method is not accurate enough is solved, an index prediction model is trained through historical mining case data, a scheme set is generated through multi-target optimization in combination with geological features of a to-be-mined unit, and then adaptive optimal mining and cutting parameters are screened, so that the mining and cutting parameter calculation accuracy is improved. And the accuracy of dynamic calculation of the mining and cutting parameters is greatly improved.

The invention discloses an application of simvastatin in preparation of a medicine for treating post-traumatic stress disorder. According to the invention, a post-traumatic stress disorder model is firstly constructed, and a result shows that in post-traumatic stress disorder model animals, obvious core behavior characteristics such as fear memory overconsolidation and anxiety behavior enhancement are shown. When simvastatin is used for intervention treatment, the fear memory fading ability of the model animal is obviously improved, and the anxiety behavior is obviously relieved; behavior experiment comprehensive evaluation shows that the stiffness time of animals in a simvastatin treatment group is remarkably shortened, the standing time and distance proportion of an open arm of an elevated cross maze are remarkably increased, the total movement distance of an open field experiment is remarkably increased, and simvastatin can effectively relieve fear memory disorder and anxiety behaviors of post-traumatic stress disorder model animals.

The application relates to the technical field of new uses of medicines, and particularly discloses application of an N-phenylpiperazine derivative in preparation of a medicine for treating Parkinson's disease, wherein the N-phenylpiperazine derivative is HS. + The N-phenylpiperazine derivative HS provided by the application can significantly improve the survival rate of SH-SY5Y cells induced by MPP + , shows a dose-dependent neuroprotective effect, can effectively improve the motor dysfunction of a model animal, increase the open field movement distance, prolong the time of staying on a rotating rod, and provides a new selection for developing an anti-Parkinson's disease treatment medicine with a neuroprotective effect.

The invention discloses a method for researching an influence mechanism of gastrodin on anxiety-like behaviors after sleep deprivation, and relates to the technical field of pharmacology and behavioral psychology. Comprising the following steps: selecting an ICR male mouse, establishing a sleep deprivation model through an improved multi-platform water environment method, and observing the influence of the expression level of autophagy related protein of the model group mouse on the anxiety-like behavior of the mouse; the method comprises the following specific steps: S1, experiment preparation and reagent preparation; s2, experimental animal grouping; s3, establishing a sleep deprivation model; s4, performing a sleep deprivation experiment and dosing; s5, performing open field experiment on the mouse; s6, performing an elevated cross maze experiment on the mouse; and S7, performing experimental statistics. According to the application, the influence of gastrodin on anxiety-like behaviors of mice after sleep deprivation is systematically discussed for the first time, the action mechanism of the gastrodin is disclosed from the perspective of autophagy related protein, and a new perspective is provided for clinical application of the gastrodin; the mouse sleep deprivation model is successfully established through an improved multi-platform water environment method, and the method is simple and easy to implement and high in repeatability.

The utility model discloses a portable rat open field experiment device, which comprises a middle block, two sides of the middle block are symmetrically hinged with a bottom plate, the top surface of the bottom plate at each side is provided with fences except for the hinged side, the front side and the rear side of the bottom plate are provided with main plates, the main plates are positioned in the fences, the bottom surface of each main plate is provided with a row of insertion blocks, and the insertion blocks are inserted into the middle block. According to the technical scheme, the whole device is detachable and foldable, after rat open field experiment operation is finished, the main plate, the side plates, the vertical bayonet sockets and the secondary plate can be detached, the bottom plates on the two sides of the middle block are folded, and the main plate, the side plates, the vertical bayonet sockets and the secondary plate can be detached, so that the rat open field experiment operation is completed. According to the open field experiment device, the two bottom plates are folded, a relatively closed space is formed between the top enclosures of the two bottom plates, the detached parts can be stored, the folded bottom plates can be connected through the connecting assemblies, finally, the effect of reducing the size of the open field experiment device can be achieved, and an operator can carry and move the device conveniently.

The application discloses an open field large model reasoning enhancement method based on probability process supervision. The application adopts reinforcement learning to perform multi-round iterative optimization on a strategy model: in each round, a dynamic golden thinking chain is generated based on a question and an answer as a reference, a plurality of exploratory paths are sampled, a step-level path loyalty reward is calculated through alignment to quantify logical effectiveness, a layered reward mechanism is constructed by combining a weighted process reward and answer correctness, and a final reward signal is used to update the model. The method does not require an external reward model and artificial labeling, realizes fine-grained process supervision through self-supervision, dynamically updates the supervision signal to adaptively improve the model, is highly versatile, and is suitable for various open reasoning tasks.

The invention discloses application of bitter gourd exosomes in regulating intestinal flora. The method comprises the following steps: establishing an Alzheimer's disease mouse model, injecting the extracted balsam pear exosome into the mouse body in a gavage manner to serve as an experimental group drug, taking normal saline as a model negative control group, detecting the change of the intestinal flora of the AD mouse by applying a 16S amplicon sequencing principle, and detecting the change of the intestinal flora metabolite of the AD mouse by applying an LS-MS technology. An open field experiment, a nesting experiment and a water maze experiment are adopted to verify the treatment effect of the balsam pear exosome on AD mouse intestinal flora and metabolic level changes thereof. Results prove that the bitter gourd exosome extracted by the invention can effectively improve the intestinal flora micro-ecology of AD mice; the compound can be used for preparing a microecological preparation for regulating intestinal flora of neurodegenerative diseases such as Alzheimer's disease, Parkinson's disease, Huntington's disease, amyotrophic lateral sclerosis, different types of spinal cerebellar ataxia and the like and diseases such as cerebral apoplexy, cerebral injury, epilepsy, tumor and the like.

The invention discloses a construction method and application of a fetal-derived autism rodent model, according to the method, a fatty acid methyl ester sulfonate solution (such as C16MES or C18MES) with the concentration of 6 mg / L is continuously given to a pregnant rodent from the 8.5 th day of pregnancy to the 21 days after offspring birth, and the influence of environmental exposure in the pregnancy period on fetal neurodevelopment is simulated. The offspring animals are subjected to multi-dimensional behavioral tests such as an open field experiment, a bead burying experiment, a self-hair combing experiment, a three-room social experiment and a new object recognition experiment, and the typical autism-like behaviors including social obstacles, inscribed plate repetition behaviors, anxiety and cognitive defects are shown. Compared with an existing gene editing or post-birth intervention model, the method targets a neural development key early-stage window period, is simple and convenient to operate and comprehensive in phenotype, and can more truly simulate an environment and gene interaction mechanism. The model can be used for screening intrauterine environment interferents and developing early diagnosis markers and therapeutic drugs.

The invention belongs to the technical field of medicines, and particularly discloses application of CL in preparation of medicines for preventing or treating depression and anxiety. Animal experiments prove that the CL can effectively improve depression behaviors of senescence model mice and anxiety behaviors (open field and elevated field) frequently accompanied by depression, can prolong the residence time of the mice in a central area in an open field experiment, and can improve the depression behavior of the mice in an elevated cross maze experiment. The number of times that the anxiety mouse enters the open arm and the time that the anxiety mouse stays in the open arm in a target area are obviously increased; the composition has no toxicity to organs such as heart, liver, spleen, lung, kidney and the like of a mouse, the weight of the mouse is not obviously reduced, and the safety is higher. The compound can be used for preparing medicines for preventing or treating depression and anxiety, and has a wide application prospect.

The application provides a spatial memory task device and a paradigm method based on a self-center and heterocentric navigation strategy. The device comprises an open field, a reward plate and a rest box. The open field is formed by a bottom plate and four side plates and has an open top end. The rest box opening is formed in the middle of the four side plates. The rest box door is installed at the rest box opening. The rest box is installed outside the side plate and is arranged correspondingly to the rest box opening. The reward wells are uniformly distributed on the reward plate. The reward wells are used for containing water. The reward plate is placed on the bottom plate. The experimental process comprises water limiting of the rats before the experiment and the experimental stage, i.e. a free exploration stage and a spatial memory task stage. The beneficial effects of the application are as follows: the paradigm method is used to measure the navigation strategy priority of the rats when the rats navigate, and to evaluate the strategy damage of the rats with Alzheimer's disease and how the rats with Alzheimer's disease navigate after the damage.

The present invention discloses a benzofuran compound in Eupatorium chinense and its use in treating depression. The benzofuran compound is isolated from the Chinese medicinal material Eupatorium chinense and named: 10 R -2-(10-methyl-10,11-dihydroxy)-5-acetyl-6-hydroxybenzofuran. The preparation method of the benzofuran compound uses the root of the Chinese medicine Eupatorium chinense as the raw material, and is obtained through steps of solvent extraction, activated carbon decolorization, macroporous adsorption column chromatography, and crystallization separation. This separation method is simple, effective, and has a high purity, and is applicable to industrial production. Through animal experiments on the benzofuran compound, behavioral data analysis such as the sucrose preference degree, tail suspension test, elevated plus maze test, forced swimming test, and open field test of mice is carried out. The benzofuran compound has a significant effect in treating depression, has a good efficacy in treating depression, and is expected to be developed into a new natural medicine for treating depression.

The invention discloses an application of aureomycin in preparation of a medicine for preventing and treating depression or anxiety behaviors. Experimental results indicate that AUN (aureusidin) can effectively increase the exploration frequency and the retention time of a model mouse on an open arm of an elevated cross labyrinth, improve the activity of the model mouse in a central area of an open field and remarkably improve depression or anxiety behaviors of the model mouse. The invention proves that the AUN has a potential effect of preventing and treating depression or anxiety behaviors, provides an experimental basis and theoretical support for developing novel medicines for treating neuropsychiatric diseases by using natural products, and has a wide application prospect.

The application belongs to the technical field of biological medicine, and specifically discloses a kind of Exosc9 The application discloses application of a gene mutation animal in construction of an anxiety model and in screening of an anti-anxiety drug. Exosc9 The gene mutation animal is a known model, but the application finds that the animal shows behavior phenotypes related to anxiety through systematic behavior experiments, including a decrease in central zone activity in an open field experiment, a decrease in open arm staying time in a high cross maze experiment, and a decrease in exploration behavior. Through behavior observation and quantitative analysis of the animal, the application can be used to study behavior characteristics of anxiety, compare phenotype differences of animals of different genotypes, genders and ages, and analyze influences of genetic and environmental factors on anxiety behavior. The application also constructs a method for screening of an anti-anxiety candidate drug and evaluation of drug efficacy based on the model, which can greatly shorten an early screening period of drug research and development, and has a wide application prospect in the field of neuropsychiatric disease research and drug research and development.

The invention discloses application of alpha-cyperone in preparation of drugs for treating sepsis, a mouse sepsis model is constructed by adopting a cecum ligation perforation (CLP) method, and through sepsis symptom clinical scoring and open field experiments, it is found that alpha-cyperone reverses the sepsis symptom, recovers the weight of a mouse and reduces the death rate of the mouse; through a Q-PCR (Quantitative Polymerase Chain Reaction) method, a Western Blot method and the like, the alpha-cyperone is found to alleviate oxidative stress and cell inflammatory factor expression, alleviate brain synaptic dysfunction and alleviate nerve cell pyroptosis in a hippocampus area; animal experiments find that alpha-cyperone has the function of improving sepsis-related encephalopathy, and in a word, a new way is provided for sepsis treatment.

The utility model discloses a mouse open field experiment device convenient to clean. The mouse open field experiment device comprises an open field platform, a supporting mechanism arranged on the open field platform, a lifting mechanism which is arranged on the outer side of the supporting mechanism and can move up and down, and a guide mechanism arranged on the open field platform. The guide mechanism comprises two guide plates arranged on the front side and the rear side of the open field platform respectively and two guide grooves formed in the opposite faces of the two guide plates respectively. Meanwhile, cleaning mechanisms capable of moving left and right along the surface of the open field platform are arranged on the two guide grooves; the open field platform has the advantages that the open field platform can be quickly switched to be opened / closed through matching of the supporting mechanism and the lifting mechanism, the open field platform can be exposed when the open field platform is in an upward moving and opening state of the test area, excrement and other impurities on the surface of the open field platform can be conveniently and quickly cleaned by the cleaning mechanism, and meanwhile experimental mice can be conveniently placed in the open field platform; and when the test area moves downwards and is closed, a mouse test open field is reconstructed.

The invention discloses a method for constructing an anxiety mouse model based on terahertz waves, relates to the technical field of anxiety animal models, and solves the problems that an anxiety animal model in the prior art depends on a behavioral test and has limitation in the aspect of accurate evaluation of a neurobiological mechanism; the related research mostly focuses on the local effect of the terahertz wave on the cells or tissues, and the system evaluation on the overall behavioristics and neurobiology influence is lacked. The method comprises the following steps: randomly distributing a plurality of mice into two groups; carrying out adaptive feeding and inverted photoperiod feeding on the two groups of mice; one of the two groups of fed mice is used as an experimental group, and the other group is used as a control group; carrying out irradiation treatment on the mice of the experimental group and the control group by constructing a platform for irradiating the mice with terahertz waves; and respectively carrying out behavioral detection of an open field experiment, an elevated cross labyrinth experiment, a three-room social experiment and a black-and-white box experiment on the mice in the control group and the experimental group after irradiation treatment, so as to solve the technical problems.

The invention provides application of Turicibacter sanguinis in preparation of a medicine for preventing and / or treating depression, and belongs to the technical field of biological medicine. Researches find that compared with depression model mice, mice treated by T. sanguinis show significant behavior improvement: sucrose consumption is increased, retention time in an interaction area in a social interaction experiment (a CD1 mouse introduction stage) is prolonged, retention time and total movement distance in a central area in an open field experiment are both increased, and the mice treated by T. sanguinis show significant behavior improvement. And meanwhile, the standing time in a forced swimming experiment is shortened. The results of the behavioral test are consistent to show that the depression-like behavior of the mouse can be obviously alleviated by the T. sanguinis intervention.

The present application belongs to the field of biological medicine and food technology, and particularly relates to application of milk-derived extracellular vesicles in improving brain health and / or improving nerve-related discomfort conditions. Compared with the prior art, the present application constructs a chronic restraint stress (CRS) induced anxiety model in adolescent mice, and uses open field test, buried bead test, tail suspension test and other behavioral experiments for functional evaluation. The results show that the milk-derived extracellular vesicles significantly improve the anxiety-like behavior of mice, indicating that the milk-derived extracellular vesicles can significantly improve the anxiety-like behavior of adolescent mice induced by chronic restraint stress (CRS) by remodeling the gut-brain axis function, and that the supplementation of milk-derived extracellular vesicles in adolescents can significantly reduce the susceptibility to anxiety in adulthood. At the same time, the present application also constructs a D-gal induced cognitive impairment model in mice, and the results show that it can reduce the production of pro-inflammatory cytokines, inhibit mucosal inflammation, thereby maintaining intestinal homeostasis and alleviating cognitive impairment.

The invention relates to the technical field of animal experiment data processing, in particular to a base line balanced random grouping and end line intelligent statistics software system for animal experiments, which comprises the following core modules: a base line data registration and overview module, a base line data analysis module and an end line analysis module, the multi-dimensional base line data correspond to eight metering indexes of six types of preset experiment plates, the six types of experiment plates comprise a sugar water test, an open field, an elevated cross, a tail suspension test (TST), forced swimming (FST) and weight, and the eight metering indexes comprise a sugar ratio (%), a total water intake (ml), a total movement distance (cm), central region time (s), arm opening time (s), FST immobile time (s), TST immobile time (s) and weight (g); according to the method, multi-index baseline equalization is realized in the grouping stage, bias is remarkably reduced, the statistical efficiency is improved, and regularized anomaly recognition is performed in the end line stage, so that subjective randomness and selective bias are reduced, and the data reliability is ensured.

A mouse model may be used in evaluation of the change in intelligence level. The mouse model may be a mouse model with high intelligence level. The mouse chromosome of the mouse model with high intelligence level at least includes all or a portion of chromosome 1 originated from wild-type mouse. The evaluation includes performing a test which may be an open field test, sucrose preference test, water maze test, space exploration Y maze test, active avoidance Y maze test, and / or fatigue rotarod test.

The invention belongs to the technical field of animal model establishment, and discloses an establishment method and application of a chronic oral ferric citrate induced Parkinson mouse model, C57BL / 6 mice are fed with mouse maintenance food (high-iron feed) added with 2.25% ferric citrate for 22 weeks, and then a 5% ferric citrate solution is used for intragastric administration for 4 weeks. Finally, behavioral evaluation is carried out through behavioral tests such as an open field test, a rotating rod test, a pole climbing test, a holding power test and a Y labyrinth test, and the tests such as the rotating rod test, the pole climbing test and the holding power test show that the PD model mouse has remarkable motor function impairment such as reduction of motor coordination, muscular tension and balance force; open field tests and Y maze show that learning and memory functions of mice are impaired, and cognitive impairments such as anxiety mood occur. Clinical chronic disease conditions of Parkinson's disease are simulated, and the chronic model is more suitable for monitoring early changes and screening early diagnosis indexes of Parkinson's disease; the method has the characteristics that the behavioral disorder can be quantified, and DA energy neuron degeneration loss characteristic lesion is realized.