10 results about "Water maze" patented technology

The invention discloses a method for extracting and preparing an oligosaccharide extract from codonopsis pilosula and application of the oligosaccharide extract in prevention and treatment of neurodegenerative disease Alzheimer's disease, and belongs to the technical field of traditional Chinese medicine pharmacy. According to the present invention, the oligosaccharide extract is enriched and prepared from Codonopsis pilosala (Franch.) Nanf., wherein the Codonopsis pilosala (Franch.) Nanf. Is a plant of the family of Platycodon grandiflorum, and the activity test of the oligosaccharide extract on the improvement of cognitive dysfunction in the animal body is performed; results show that in a mouse cognitive function impairment model caused by sleep deprivation, the high-dose codonopsis pilosula oligosaccharide has a prominent effect on improving the learning and memory ability of a Morris water maze and a neuroprotective effect, the efficacy of the codonopsis pilosula oligosaccharide is equivalent to that of a positive control drug manntranet, and no obvious toxic or side effect is found. The codonopsis pilosula oligosaccharide is expected to be further developed into functional food, health care products and / or medicines with the effect of preventing and / or improving cognitive impairment.

The invention relates to the field of medicinal chemistry, and particularly discloses a piperidine derivative for neurodegenerative diseases, a pharmaceutical composition and application of the piperidine derivative. The piperidine derivative has a structure shown in a general formula (I). In-vivo experiments prove that the compound can effectively improve learning and memory impairment of beta-amyloid protein induced Alzheimer disease model rats, the escape latency is remarkably shortened and the ring penetration frequency is increased in a Morris water maze test, and the behavioral improvement effect of the compound is remarkably superior to that of carristine and can be compared favorably with that of a positive control drug. Meanwhile, the series of compounds can significantly inhibit abnormally increased acetylcholin esterase activity in hippocampal tissues of model rats, have an excellent regulation effect on cholinergic system functions, and show a synergistic effect of cognitive improvement and key pathological link regulation. The compound provided by the invention provides a new and potential candidate drug for treatment of Alzheimer's disease.

This utility model relates to the field of animal maze technology, specifically to an animal water entry system for the Morris water maze experiment, aiming to solve the technical problems of tracking anomalies caused by manual release and time errors caused by human collaboration. The system includes a pool, pipes, an electrically controlled gate, a pedal, and a computer. The pool is filled with water, the pipes are installed on the side wall of the pool, the electrically controlled gate controls the opening and closing of the pipe openings, the pedal controls the electrically controlled gate, and the computer has built-in experimental software for recording experiments. Pipes are installed on the side wall of the pool, and experimental animals are placed inside the pipes. The electrically controlled gate prevents experimental animals from entering the pool. At the start of the experiment, the pedal is pressed, which simultaneously controls the opening of the electrically controlled gate and the start of the experimental software in the computer to avoid time errors. Furthermore, because an electrically controlled gate is used, it avoids the light shadows caused by manually placing experimental animals, thus preventing tracking anomalies.

There is disclosed an apparatus for producing a water maze from walls of falling water droplets. The apparatus may comprise a plurality of tubular spray bars each perforated with a water inlet for receiving pressurized water. A series of output holes may extend along a length of each spray bar through which the received water is discharged to produce the droplets. A plurality of connectors may be configured to join two or more of the spray bars at one or more vertices in an array producing the water maze. A flow regulator may be disposed between the water inlet and the series of outlet holes for resistively spreading the inlet water evenly across the length of the spray bar prior to the discharging. A 3D printing of the connectors may facilitate a wide variety of horizontal angles required between spray bars joining at the vertices in one-of-a-kind maze designs.

The invention discloses a method for researching a mild cognitive impairment action mechanism of a cornu cervi pantotrichum polypeptide based on a Wnt / beta-catenin signal channel, relates to the technical field of mechanism analysis, and solves the problem of difficulty in realizing spanning from basic research to clinical transformation in the prior art. Randomly dividing the rats into five groups of blank control, model, positive control and VAP high / low dose, continuously performing intragastric administration on each group for 18 days, and feeding distilled water into the blank and model groups; 2 mg / kg scopolamine is injected into abdominal cavities of the model group after 18 days and 2 hours, and normal saline is injected into the blank group; the scopolamine model is used for measuring the 2min static / motion time of the rat by using an open field experiment, and the unilateral common carotid artery ligation model is used for measuring the escape latency / platform penetration times by using a water maze; the method comprises the following steps: taking blood, separating a hippocampal region, and carrying out HE dyeing, ELISA and Western blot; sPSS 21.0 analysis and single factor variance analysis are used for evaluating the difference and Plt; 0.05 or Plt; 0.01 has statistical significance.

This invention utilizes *Allium macrostemon* to obtain its aqueous extract, petroleum ether extract, dichloromethane extract, ethyl acetate extract, and n-butanol extract. The effects of these aqueous extracts and extracts of different polarities on the learning and memory functions of D-galactose-induced aging model mice were investigated using Y-maze, darkness avoidance, Morris water maze, and laser speckle cerebral blood flow assays. Reactive oxygen species (ROS), SOD, and MDA were also measured. Multiple experiments demonstrated that *Allium macrostemon* can improve memory function in mice. Furthermore, the effects of *Allium macrostemon* extract on arecoline- or oxidative tremor-induced tremor behavior in mice were studied. The results showed that *Allium macrostemon* extract can effectively shorten the duration of tremors, proposing a new application for *Allium macrostemon* extract in the preparation of drugs for the prevention and / or treatment of neurodegenerative diseases, providing broad application prospects for the treatment or adjuvant therapy of neurodegenerative diseases.

The application belongs to the technical field of new use of medicines, and provides application of Zhongwuning in preparation of anti-aging medicines. It is proved through experimental research that Zhongwuning can play an anti-aging role in multiple ways. Zhongwuning can significantly increase the spleen and thymus coefficients of D-galactose-induced aging model mice, significantly reduce the MDA content activity of D-galactose-induced aging model mice, and improve the activities of SOD and GSH-Px of D-galactose-induced aging model mice. Zhongwuning can significantly reduce the body inflammation level, significantly reduce the LPO and LF contents and the MAO-B activity in the brain tissue of D-galactose-induced aging model mice, and slow down the loss of hydroxyproline and hyaluronic acid in the aging skin of D-galactose-induced aging model mice. The Morris water maze experiment test results show that Zhongwuning has the effect of improving the learning and memory abilities of the aging model mice. Therefore, Zhongwuning has a certain application prospect in preparation of anti-aging medicines.

This invention discloses a traditional Chinese medicine composition for the prevention, mitigation, or treatment of Alzheimer's disease, comprising 8-12 parts of Cistanche deserticola, 8-12 parts of Polygonatum sibiricum, 8-12 parts of Panax ginseng, 12-18 parts of Astragalus membranaceus, 12-18 parts of Angelica sinensis, 12-18 parts of Ligustrum lucidum, 8-12 parts of Salvia miltiorrhiza, and 8-10 parts of Panax notoginseng, all by weight. The traditional Chinese medicine composition of this invention significantly improves Alzheimer's disease-related symptoms in a mouse model of Alzheimer's disease, including the novel object recognition index, Y-maze spatial cognition index, water maze activity index, number and distribution of Nissl bodies in neurons, and β-amyloid protein deposition. It is expected to effectively prevent, mitigate, or improve different stages of Alzheimer's disease and its related complications and underlying diseases.

The utility model relates to the technical field of animal behavior experiment equipment, and provides a water maze platform suitable for tree shrews, which comprises a transparent acrylic material platform, a waterproof shell, a lifting device and an intelligent detection control system. The platform adapts to the body type of the tree shrew; an attitude sensor is arranged at the bottom of the platform and communicates with a control circuit board through a waterproof connecting line, the load change of the platform is detected in real time, the lifting device is controlled to slowly jack the platform for a period of time and then automatically reset, and lifting time data are synchronously transmitted to a terminal through a wireless transmission module. The lifting device adopts a screw rod to drive a sleeve to drive the platform to lift, and the waterproof effect of the device is ensured by combining an oil seal in a waterproof shell and a thread sealing design; #-shaped grooves or raised lines are arranged on the surface of the platform so as to enhance friction force and regional identifiability. Through the platform lifting triggered by animal behaviors, the problems that a traditional water maze platform is poor in adaptability to tree shrews and lacks intelligent interaction are solved, and the experiment efficiency and precision are remarkably improved.

This invention discloses the application of Polygonatum odoratum carbon quantum dots (PC-CQDs) in the preparation of drugs for treating vascular dementia, belonging to the field of pharmaceutical technology. This invention is the first to apply PC-CQDs to the treatment of vascular dementia. Morris water maze test and hippocampal histopathological analysis confirmed that PC-CQDs can significantly improve spatial learning and memory abilities in VaD rats, protect hippocampal neurons, and exhibit good biocompatibility. This invention provides a new drug option for the treatment of vascular dementia, possessing significant clinical application value and broad market prospects.