26 results about "Water maze" patented technology

The invention discloses a method for extracting and preparing an oligosaccharide extract from codonopsis pilosula and application of the oligosaccharide extract in prevention and treatment of neurodegenerative disease Alzheimer's disease, and belongs to the technical field of traditional Chinese medicine pharmacy. According to the present invention, the oligosaccharide extract is enriched and prepared from Codonopsis pilosala (Franch.) Nanf., wherein the Codonopsis pilosala (Franch.) Nanf. Is a plant of the family of Platycodon grandiflorum, and the activity test of the oligosaccharide extract on the improvement of cognitive dysfunction in the animal body is performed; results show that in a mouse cognitive function impairment model caused by sleep deprivation, the high-dose codonopsis pilosula oligosaccharide has a prominent effect on improving the learning and memory ability of a Morris water maze and a neuroprotective effect, the efficacy of the codonopsis pilosula oligosaccharide is equivalent to that of a positive control drug manntranet, and no obvious toxic or side effect is found. The codonopsis pilosula oligosaccharide is expected to be further developed into functional food, health care products and / or medicines with the effect of preventing and / or improving cognitive impairment.

The invention relates to the technical field of learning and memory ability evaluation, and provides a mouse hippocampus learning and memory ability comprehensive evaluation method and system based on a Morris water maze, the method is different from a traditional method for evaluating learning and memory only through an incubation period, a mouse behavior character stability and swimming skill dual evaluation mechanism is innovatively introduced, and the method is suitable for comprehensive evaluation of learning and memory ability of a mouse hippocampus. And in combination with a training adaptation period, behavior path analysis and weighted scoring of multi-dimensional behavior indexes, precise quantification of the hippocampus dependency space learning and memory ability is realized. The experimental indexes comprise swimming speed, path length, target quadrant residence time and incubation period, and performing standardized calculation after weight assignment to generate a memory ability evaluation score. According to the invention, the scientificity, stability and experimental repeatability of an evaluation result are improved, and the method is suitable for mouse cognitive function evaluation under various research situations such as drug intervention, neurodegenerative disease models and the like.

The invention discloses a method for synergistically treating Alzheimer's disease through gene-modified human interdental stem cells, and belongs to the field of drug therapy. According to the method, healthy adult dental pulp mesenchymal stem cells (hDPSCs) are extracted, BDNF and NRF2 genes are introduced through lentiviral vectors, the BDNF and NRF2 genes have neurotrophic, antioxidant and anti-inflammatory capacities, and then the hDPSCs are combined with curcumin nanoparticles and injected to the hippocampus of an AD mouse through stereotactic injection. The BDNF can promote nerve regeneration, the NRF2 can improve oxidative stress and inflammation environment, and the curcumin nanoparticles regulate A beta metabolism and activate a BDNF signal channel. Experiments show that the combined therapy can significantly enhance hippocampal neurogenesis of AD mice and improve expression of neuron markers, and it is proved through Morse water maze tests that the combined therapy can improve the spatial learning and memory ability of the mice. The method breaks through the limitation of traditional single-target treatment, multi-dimensional pathological intervention is achieved, the stem cells are rich in source and high in safety, and a new strategy is provided for treatment of the Alzheimer's disease.

The invention discloses a method for constructing a diabetic encephalopathy mouse model through combined induction of cadmium and fructose. According to the method, the diabetes contamination dosage is promoted by adjusting cadmium; the condition that the fasting blood glucose of the mouse is greater than or equal to 11.1 mmol / L, urine glucose inspection is positive, and mine field experiments, new object recognition experiments and water maze mouse learning and memory experiments verify that the cognitive dysfunction of the mouse successfully induces the diabetic encephalopathy. According to the method, through combined induction of the fructose solution and cadmium, the modeling condition is met, and the cost is reduced. The model manufacturing process has little harm to animal bodies and conforms to the international and domestic animal welfare principle.

The invention belongs to the technical field of animal experiments, and particularly relates to a mouse visual water maze task training device and method, during training, a control system controls two display stimulation mechanisms to display different stimulation images respectively, a mouse starts from an initial area, the different stimulation images are observed in an observation choice area, and the mouse visual water maze task is trained. The detection mechanism detects the termination block into which the mouse enters, the control system judges the selection condition of the mouse according to the stimulation image and the detection result of the detection mechanism, and if the selection is correct, the safety mechanism is started for the mouse to stay, and if the selection is wrong, the control system does not act. According to the method, the instinct that the mouse seeks a safety platform in water is utilized, so that the mouse can quickly establish neural association between a stimulation target and a final space positioning target under the condition that the mouse is not trained or is simply trained, the visual training task of the mouse can be quickly adapted, the training efficiency is improved, and the workload of workers is reduced.

The invention relates to application of an exosome derived from olfactory mucosa mesenchymal stem cells pretreated based on active peptide in improvement of neuroinflammation, in particular to a treatment strategy for neuroinflammation of Alzheimer's disease (AD). An IFN-FGF fusion protein (SEQ ID NO: 3) and a coding gene (SEQ ID NO: 4) of the IFN-FGF fusion protein are constructed, and the IFN-FGF fusion protein and the coding gene (SEQ ID NO: 4) are used for in-vitro pretreatment of OM-MSCs, so that the A-MS Cs-Exo exosome with anti-inflammatory and neuroprotective functions is obtained. In an A beta oligomer-induced AD neuroinflammation model, the A-MSCs-Exo significantly alleviates neuroinflammation and cognitive impairment by inhibiting excessive activation of hippocampal Iba1 + microglia cells, reducing serum IL-6 level, improving SOD activity and improving Morris water maze behavioral performance, the effect is better than that of traditional FGF-1 pretreatment (B-MSCs-Exo) and untreated exosome (C-MSCs-Exo), and the A-MSCs-Exo can be used for preparing the A beta oligomer-induced AD neuroinflammation model. The invention provides a novel exosome intervention strategy for AD treatment.

The utility model relates to the technical field of driving devices in floor washing equipment, in particular to a waterproof labyrinth ring, a driving structure with the waterproof labyrinth ring and floor washing equipment, the waterproof labyrinth ring comprises a ring body, and the ring body comprises a driving end matching surface and an executing end matching surface; at least the execution end matching surface is provided with a first waterproof groove which is annularly arranged; the ring body is further provided with a mounting hole, and the mounting hole and the ring body are coaxially arranged. The waterproof labyrinth ring is arranged and applied to the driving structure in the scrubber, the driving end matching face is used for facing the motor side of the driving end, the execution end matching face is used for facing the movable impeller side of the execution end, and in the application scene, the first waterproof groove formed in the execution end matching face is in an inverted buckle shape; and external moisture is effectively prevented from invading the bearing, and the long-term stable and smooth operation of the bearing is ensured.

The application relates to the technical field of experimental instruments, and discloses an intelligent vibration maze experiment device capable of replacing a water maze experiment, which comprises an outer barrel module, an intelligent detection module box, an inner barrel module, an outlet module, an inlet module and an intelligent robot dog, the inner barrel module is fixed on the upper fixing seat of the inner barrel fixing spring of the outer barrel through a spring fixing seat fixing screw, the square hole at the upper portion of the inner barrel module is sleeved on the high-foam rubber sealing ring of the cantilever of the outer barrel, the intelligent detection module box is fixed on the cantilever at the upper portion of the outer barrel through a screw, the inlet module is inserted into the square hole at the middle portion of the outer barrel through the inlet of the outlet module, and the electric push rod C is fixed on the upper edge of the square hole at the middle portion of the outer barrel through an electric push rod C fixing screw. The water maze experiment is replaced by the mode of simulating a seismic environment, so that the risk of animal drowning is avoided, and the life safety of the animals is greatly guaranteed. Since no water is involved, the animals do not have the risk of wound infection after entering the water.

The invention relates to a tx-j mouse animal model construction method based on phlegm-blood stasis syndrome cognitive impairment, and belongs to the technical field of medicine. The tx-j mouse is firstly fed with high-fat diet to make a hyperlipidemia state, then an ice-water bath method is adopted to make a'blood stasis' model, and finally the tx-j mouse animal model for the cognitive impairment of the syndrome of intermingled phlegm and blood stasis is constructed. After the model is successfully constructed, firstly observing the state and tongue color of the mouse, carrying out a water maze experiment, then detecting blood rheology indexes, blood fat indexes, tissue and serum copper contents and inflammatory factors, and observing brain pathological changes of the mouse. The high-fat feed is used for feeding to make a hyperlipidemia state, which is equivalent to'turbid phlegm 'of traditional Chinese medicine. The'blood stasis' model is manufactured by adopting an ice-water bath method on the basis of the traditional Chinese medicine theory of'cold coagulation and blood stasis', the modeling mode makes up the defects of the WD cognitive impairment model without combination of diseases and syndromes at present, and an effective experimental animal model is provided for revealing the pathogenesis of hepatolenticular degeneration cognitive impairment and evaluating the curative effect of traditional Chinese medicine.

The invention discloses an AD rat model imaging experiment method, a QSM graph construction method and QSM application, which can be used for researching pathological protein change in a hippocampus area. The rat model imaging experiment method comprises the following steps: (I) respectively injecting equal amounts of A [beta] 1-40 and PBS solutions into hippocampus CA1 regions on two sides of an SD rat to construct an A [beta]-induced AD rat model G1 and a control group G2; (II) replacing an A beta sequence in a rat App gene and mutation sites around the A beta sequence with a human source sequence; (III) evaluating the spatial orientation learning and memory ability of the rat by using a Morris water maze experiment which is carried out for 6 days in total and is divided into two stages: the first stage is a platform hiding stage test for 5 days; and the second stage is a platform-free space exploration stage test for one day.

The utility model discloses an adjustable and controllable water maze behavior testing device, which relates to the technical field of water maze experiments and comprises a main cylinder and a testing component, support column feet are mounted at the bottom of the main cylinder, and the testing component is arranged outside the main cylinder. The testing assembly comprises a miniature water pump, a liquid guide pipe, a liquid level sensor, telescopic adjusting rods, a mounting frame, an infrared camera, an ultrasonic locator and a physiological sensor, the liquid guide pipe is connected to the exterior of the miniature water pump, the liquid level sensor is arranged on the right side in the main cylinder, and the telescopic adjusting rods are additionally arranged at the four corners of the upper end of the main cylinder; a mounting frame is arranged at the upper end of the telescopic adjusting rod, an infrared camera is mounted in the middle of the lower end of the mounting frame, and an ultrasonic positioner is arranged on the rear side in the main barrel. Compared with a traditional single-parameter water maze test, the adjustable and controllable water maze behavior test device is more refined, data are more comprehensive, manual judgment errors are reduced, and experiment repeatability and accuracy are improved.

The invention belongs to the technical field of functional probiotic preparation, and provides a strain capable of improving memory and cognitive ability and a preparation method of the strain. A double-layer core-shell carrier enrichment culture design of a zinc-magnesium complex nano core-chitosan inner layer-chitosan sodium alginate outer layer is adopted, the carrier is added into an improved MRS culture medium according to the proportion of 0.2-0.3%, bifidobacterium breve BB01 is cultured for 120 hours, and then collection and stabilization treatment are performed. According to the present invention, the efficient enrichment of the intracellular zinc content of 280-380 [mu] g / g and the magnesium content of 160-230 [mu] g / g of the strain is achieved, the gamma-aminobutyric acid yield and the butyric acid yield are respectively increased by 65-75% and 52-62%, the Morris water maze platform searching time is shortened by 40-60%, and the plasma zinc and magnesium concentrations are respectively increased by 15-25% and 10-18%. The technical problem that the traditional probiotics have insufficient effects in the aspects of improving cognitive function and enhancing memory is solved, and the probiotics have wide application value in the fields of health foods, functional foods, neurotrophic supplements and related medical health products.

The invention discloses an application of H1152 in preparation of a medicine for treating metabolism-related cognitive impairment, belongs to the technical field of medicine, breaks through the limitation that an existing medicine singly regulates metabolism or cognition, and the H1152 can synchronously inhibit abnormal weight growth induced by high fat diet and remarkably improve spatial memory water maze experiments and short-term memory new object recognition experiments. Dual intervention of metabolism and nerves is realized; compared with statins, the H1152 does not observe creatine kinase rise or liver enzyme abnormity under the equivalent lipid-lowering dosage, and animal experiments show that the H1152 has better safety; the ROCK signal channel is regulated and controlled, and the ROCK signal channel acts on vascular endothelial function improvement and neuronal synaptic plasticity enhancement at the same time, so that the problem of co-disease of metabolism and cognition is solved from the multi-target level; the body weight growth rate, the water maze escape latency and the new object recognition index are integrated as the combined curative effect markers for the first time, and quantifiable objective standards are provided for clinical curative effect evaluation.

This invention provides a method for identifying compounds for treating delayed neurological injury in the chronic phase of cerebral hemorrhage. The method includes establishing a mouse model of cerebral hemorrhage, administering the identified drug to a treatment group and immunoglobulin IgG to a non-treatment group; assessing motor dysfunction through neurological function evaluation and a turning angle test; evaluating the excitability of thalamic neurons using whole-cell patch-clamp recording technology; comparing synaptic transmission function in the treatment and non-treatment groups; assessing spatial learning and memory abilities using the Morris water maze test; and comprehensively evaluating the improvement in neurological function, synaptic transmission, and cognitive function to determine the therapeutic effect of the drug. The method also includes collecting autopsy brain tissue from cerebral hemorrhage patients and healthy controls, performing spatial transcriptome sequencing to identify differentially expressed genes, and exploring the pathogenic mechanism in the animal model of cerebral hemorrhage. This method can accurately screen and identify potential therapeutic drugs for chronic neurological injury in cerebral hemorrhage.

The invention relates to the field of medicinal chemistry, and particularly discloses a piperidine derivative for neurodegenerative diseases, a pharmaceutical composition and application of the piperidine derivative. The piperidine derivative has a structure shown in a general formula (I). In-vivo experiments prove that the compound can effectively improve learning and memory impairment of beta-amyloid protein induced Alzheimer disease model rats, the escape latency is remarkably shortened and the ring penetration frequency is increased in a Morris water maze test, and the behavioral improvement effect of the compound is remarkably superior to that of carristine and can be compared favorably with that of a positive control drug. Meanwhile, the series of compounds can significantly inhibit abnormally increased acetylcholin esterase activity in hippocampal tissues of model rats, have an excellent regulation effect on cholinergic system functions, and show a synergistic effect of cognitive improvement and key pathological link regulation. The compound provided by the invention provides a new and potential candidate drug for treatment of Alzheimer's disease.

This utility model relates to the field of animal maze technology, specifically to an animal water entry system for the Morris water maze experiment, aiming to solve the technical problems of tracking anomalies caused by manual release and time errors caused by human collaboration. The system includes a pool, pipes, an electrically controlled gate, a pedal, and a computer. The pool is filled with water, the pipes are installed on the side wall of the pool, the electrically controlled gate controls the opening and closing of the pipe openings, the pedal controls the electrically controlled gate, and the computer has built-in experimental software for recording experiments. Pipes are installed on the side wall of the pool, and experimental animals are placed inside the pipes. The electrically controlled gate prevents experimental animals from entering the pool. At the start of the experiment, the pedal is pressed, which simultaneously controls the opening of the electrically controlled gate and the start of the experimental software in the computer to avoid time errors. Furthermore, because an electrically controlled gate is used, it avoids the light shadows caused by manually placing experimental animals, thus preventing tracking anomalies.

There is disclosed an apparatus for producing a water maze from walls of falling water droplets. The apparatus may comprise a plurality of tubular spray bars each perforated with a water inlet for receiving pressurized water. A series of output holes may extend along a length of each spray bar through which the received water is discharged to produce the droplets. A plurality of connectors may be configured to join two or more of the spray bars at one or more vertices in an array producing the water maze. A flow regulator may be disposed between the water inlet and the series of outlet holes for resistively spreading the inlet water evenly across the length of the spray bar prior to the discharging. A 3D printing of the connectors may facilitate a wide variety of horizontal angles required between spray bars joining at the vertices in one-of-a-kind maze designs.

The invention belongs to the technical field of nano-emulsions, and particularly relates to a nano-emulsion for preventing Alzheimer's disease and a preparation method of the nano-emulsion. The nano-emulsion is prepared from the following raw materials in parts by weight: 11 to 22 parts of oil, 1.11 to 3.50 parts of an emulsifier, 1 to 3 parts of a processing aid, 0.31 to 0.86 part of a functional component, 0.01 to 0.03 part of an antioxidant and 70 to 90 parts of water, wherein the oil comprises acer truncatum seed oil and xanthoceras sorbifolia bunge oil. The acer truncatum seed oil and the xanthoceras sorbifolia bunge oil are reasonably matched and have a synergistic effect with functional ingredients, and it is found through a Morris water maze experiment that the learning ability of mice can be improved; mouse experiments show that the SOD activity of brain tissues, liver tissues and serum of mice with Alzheimer's disease can be improved, the activity of MDA (malondialdehyde) can be reduced, the ChAT activity of the brain tissues of the mice can be improved, and the activity of AChE can be reduced, so that the symptoms of the Alzheimer's disease can be prevented / relieved.

The invention belongs to the technical field of biomedicine, and discloses a GluN2B single-domain antibody as well as a fusion protein, a related biological material and application thereof. The amino acid sequence of the GluN2B single-domain antibody disclosed by the invention comprises a sequence as shown in SEQ ID No.1 or a sequence which has at least 80% of identity with the sequence as shown in SEQ ID No.1. The GluN2B single-domain antibody has the advantages of high characteristics, high affinity, strong tissue penetrating ability and the like, and the fusion protein obtained after the GluN2B single-domain antibody is fused with a humanized tag shows a remarkable anti-cerebral ischemia effect in vivo and in vitro, for example, the GluN2B single-domain antibody can be used for remarkably reducing the cerebral infarction volume of MCAO rats, the nerve function defect score and the crawling dislocation rate in a grid experiment; the total movement distance, the central area movement distance, the central area detention time and the number of times of entering the central area of MCAO animals are remarkably increased, and the target quadrant path proportion, the detention time and the number of times of crossing the platform in the water maze experiment are remarkably increased. The invention can be applied to the field of preparation of medicaments for treating cerebral ischemia.

The invention discloses a method for researching a mild cognitive impairment action mechanism of a cornu cervi pantotrichum polypeptide based on a Wnt / beta-catenin signal channel, relates to the technical field of mechanism analysis, and solves the problem of difficulty in realizing spanning from basic research to clinical transformation in the prior art. Randomly dividing the rats into five groups of blank control, model, positive control and VAP high / low dose, continuously performing intragastric administration on each group for 18 days, and feeding distilled water into the blank and model groups; 2 mg / kg scopolamine is injected into abdominal cavities of the model group after 18 days and 2 hours, and normal saline is injected into the blank group; the scopolamine model is used for measuring the 2min static / motion time of the rat by using an open field experiment, and the unilateral common carotid artery ligation model is used for measuring the escape latency / platform penetration times by using a water maze; the method comprises the following steps: taking blood, separating a hippocampal region, and carrying out HE dyeing, ELISA and Western blot; sPSS 21.0 analysis and single factor variance analysis are used for evaluating the difference and Plt; 0.05 or Plt; 0.01 has statistical significance.

This invention utilizes *Allium macrostemon* to obtain its aqueous extract, petroleum ether extract, dichloromethane extract, ethyl acetate extract, and n-butanol extract. The effects of these aqueous extracts and extracts of different polarities on the learning and memory functions of D-galactose-induced aging model mice were investigated using Y-maze, darkness avoidance, Morris water maze, and laser speckle cerebral blood flow assays. Reactive oxygen species (ROS), SOD, and MDA were also measured. Multiple experiments demonstrated that *Allium macrostemon* can improve memory function in mice. Furthermore, the effects of *Allium macrostemon* extract on arecoline- or oxidative tremor-induced tremor behavior in mice were studied. The results showed that *Allium macrostemon* extract can effectively shorten the duration of tremors, proposing a new application for *Allium macrostemon* extract in the preparation of drugs for the prevention and / or treatment of neurodegenerative diseases, providing broad application prospects for the treatment or adjuvant therapy of neurodegenerative diseases.

The application belongs to the technical field of new use of medicines, and provides application of Zhongwuning in preparation of anti-aging medicines. It is proved through experimental research that Zhongwuning can play an anti-aging role in multiple ways. Zhongwuning can significantly increase the spleen and thymus coefficients of D-galactose-induced aging model mice, significantly reduce the MDA content activity of D-galactose-induced aging model mice, and improve the activities of SOD and GSH-Px of D-galactose-induced aging model mice. Zhongwuning can significantly reduce the body inflammation level, significantly reduce the LPO and LF contents and the MAO-B activity in the brain tissue of D-galactose-induced aging model mice, and slow down the loss of hydroxyproline and hyaluronic acid in the aging skin of D-galactose-induced aging model mice. The Morris water maze experiment test results show that Zhongwuning has the effect of improving the learning and memory abilities of the aging model mice. Therefore, Zhongwuning has a certain application prospect in preparation of anti-aging medicines.

This invention discloses a traditional Chinese medicine composition for the prevention, mitigation, or treatment of Alzheimer's disease, comprising 8-12 parts of Cistanche deserticola, 8-12 parts of Polygonatum sibiricum, 8-12 parts of Panax ginseng, 12-18 parts of Astragalus membranaceus, 12-18 parts of Angelica sinensis, 12-18 parts of Ligustrum lucidum, 8-12 parts of Salvia miltiorrhiza, and 8-10 parts of Panax notoginseng, all by weight. The traditional Chinese medicine composition of this invention significantly improves Alzheimer's disease-related symptoms in a mouse model of Alzheimer's disease, including the novel object recognition index, Y-maze spatial cognition index, water maze activity index, number and distribution of Nissl bodies in neurons, and β-amyloid protein deposition. It is expected to effectively prevent, mitigate, or improve different stages of Alzheimer's disease and its related complications and underlying diseases.

The utility model relates to the technical field of animal behavior experiment equipment, and provides a water maze platform suitable for tree shrews, which comprises a transparent acrylic material platform, a waterproof shell, a lifting device and an intelligent detection control system. The platform adapts to the body type of the tree shrew; an attitude sensor is arranged at the bottom of the platform and communicates with a control circuit board through a waterproof connecting line, the load change of the platform is detected in real time, the lifting device is controlled to slowly jack the platform for a period of time and then automatically reset, and lifting time data are synchronously transmitted to a terminal through a wireless transmission module. The lifting device adopts a screw rod to drive a sleeve to drive the platform to lift, and the waterproof effect of the device is ensured by combining an oil seal in a waterproof shell and a thread sealing design; #-shaped grooves or raised lines are arranged on the surface of the platform so as to enhance friction force and regional identifiability. Through the platform lifting triggered by animal behaviors, the problems that a traditional water maze platform is poor in adaptability to tree shrews and lacks intelligent interaction are solved, and the experiment efficiency and precision are remarkably improved.

This invention discloses the application of Polygonatum odoratum carbon quantum dots (PC-CQDs) in the preparation of drugs for treating vascular dementia, belonging to the field of pharmaceutical technology. This invention is the first to apply PC-CQDs to the treatment of vascular dementia. Morris water maze test and hippocampal histopathological analysis confirmed that PC-CQDs can significantly improve spatial learning and memory abilities in VaD rats, protect hippocampal neurons, and exhibit good biocompatibility. This invention provides a new drug option for the treatment of vascular dementia, possessing significant clinical application value and broad market prospects.

The invention belongs to the technical field of medicines, and particularly discloses application of a fructus gardeniae-cortex cinnamomi composition in preparation of a medicine for improving learning and memory impairment. A rat insomnia model is induced by adopting p-chlorophenylalanine, interference is given to diazepam and a fructus gardeniae-cinnamon medicine pair, an open field experiment and a Morris water maze experiment are carried out, and the medicine effect of the fructus gardeniae-cinnamon medicine pair on improving insomnia is investigated; experimental results show that the cape jasmine-cinnamon composition can improve the learning and memory impairment of an insomnia rat model caused by p-chlorophenylalanine, has an obvious effect of improving the learning and memory impairment, and can be used for preparing the medicine for improving the learning and memory impairment. The invention provides a new drug source for improving learning and memory ability impairment.