The present invention discloses a special feed for laboratory mice and a preparation method thereof, and relates to the technical field of laboratory animal feed. The special feed for experiment mice is composed of the following raw materials by weight: 30-40 parts of corn, 20-40 parts of flour, 20-40 parts of rice, 20-40 parts of soybean, 10-20 parts of fish meal, 5-8 parts of elderberry powder, 3-5 parts of eggshell powder, 1-3 parts of lotus powder, 3-5 parts of sucrose, 1-3 parts of fructose, 1-3 parts of vegetable oil, 1-3 parts of lard, 0.1-0.3 parts of a mineral salt premix, 0.1-0.3 part of an amino acid premix, 0.1-0.2 part of a vitamin premix, and 0.1-0.2 part of a trace element premix. The feed employs high quality ingredients in reasonable ratio, not only meets the nutritional needs of growth and development of laboratory mice but also is free of heavy metals and pesticide residues, so as to ensure the health of the mice, and avoid affects on experimental results.