176 results about "Laboratory mouse" patented technology

The invention discloses a gRNA sequence. The sequence is capable of editing a DNA sequence in the manner of taking a hepatitis B viral genome specific locus as a target sequence. The invention also discloses a CRISPR-SaCas9 system containing the gRNA sequence and a recombinant adeno-associated virus packaged with the system. The system and the packaging virus show higher HBV scavenging activity in cells and in transgenic mice body. On the 38th day after twice continuous high dose injection, the contents of HBsAg and HBeAg in experimental group mice serum, compared with the control group, are respectively reduced by 62.96+ / -7.59% and 65.18+ / -3.08%; the HBV DNA content in the serum, compared with the control group, is reduced by 92.82+ / -3.67%; the liver and other visceral organs of the experimental mice are all free from any pathologic change, the off-target effect is also not detected and the application prospects of the gRNA sequence and the corresponding CRISPR-SaCas9 system provided by the invention in preparing the hepatitis B therapeutics are shown.

The invention discloses an application of whey protein peptide in the preparation of medicines, health foods or foods for improving learning memory. Whey protein peptide has the characteristics of easy absorption, fast absorption, low viscosity, good water solubility and the like. The whey protein peptide can promote a laboratory mouse to establish active avoidance of conditioned response, slows down the memory decay speed of passive avoidance of conditioned response, and simultaneously improves the space learning memory capability of the laboratory mouse. The whey protein peptide has the efficacy of improving learning memory and has certain scientific value and economic benefit when applied to the preparation of medicines, health foods or foods for improving learning memory. The invention provides a new approach for improving learning memory by means of diet.

The invention provides a construction and an application of recombinant human interleukin 12 eukaryotic expression vector and relates to the development of recombinant human interleukin 12 (rhIL-12) eukaryotic expression vector, the screening of stable expression cell lines and the adjuvant function of nucleic acid vaccine gene and the therapeutical effect of tumor gene. Ten hydrophobic flexible amino acid joints are used to connect P40 and P35 to obtain fusion gene to construct rhIL-12 eukaryotic expression plasmid (pCDNA6-IL-12), then the expression plasmid is transferred to CHO cell and finally stable expression cell lines are screened. The expression product has good biological activity. Nucleic acid vaccine and pCDNA6-IL-12 are used for coimmune so as to significantly increase the immune effect. Strong antitumor effect can be exerted when the expression vector of the invention is directly injected inside the tumor of laboratory mouse tumor model or is first used to perform gene modification to tumor cells and then implanted in tumor. The expression vector of the invention can be used in the search of clinical trial stage after further perfecting.

Noninvasive, MR-compatible methods and systems optically detect mechanical cardiac activity by anatomic (e.g., esophageal) movements. Most preferably, esophageal motion is detected optically and is indicative rhythmic cardiac activities. This esophageal motion may then be detected and used to provide a signal indicative of periods of cardiac activity and inactivity. The signal may be further processed so as to generate a trigger signal that may be input to a MR scanner. In such a manner, MR microscopy may be accomplished to acquire information at a specific phase of the cardiac cycle, for example, in synchrony with periods of cardiac inactivity. Moreover, since mechanical cardiac activity is detected and employed, instead of electrical activity as is employed in conventional techniques, the present invention is immune to electromagnetic interference during MR microscopy. As a result, robust cardiac signals may be monitored and gated during 2-dimensional and 3-dimensional in vivo microscopy. The present invention is therefore especially well suited for MR microscopy of small animals, such as laboratory mice and rats.

The present invention discloses a special feed for laboratory mice and a preparation method thereof, and relates to the technical field of laboratory animal feed. The special feed for experiment mice is composed of the following raw materials by weight: 30-40 parts of corn, 20-40 parts of flour, 20-40 parts of rice, 20-40 parts of soybean, 10-20 parts of fish meal, 5-8 parts of elderberry powder, 3-5 parts of eggshell powder, 1-3 parts of lotus powder, 3-5 parts of sucrose, 1-3 parts of fructose, 1-3 parts of vegetable oil, 1-3 parts of lard, 0.1-0.3 parts of a mineral salt premix, 0.1-0.3 part of an amino acid premix, 0.1-0.2 part of a vitamin premix, and 0.1-0.2 part of a trace element premix. The feed employs high quality ingredients in reasonable ratio, not only meets the nutritional needs of growth and development of laboratory mice but also is free of heavy metals and pesticide residues, so as to ensure the health of the mice, and avoid affects on experimental results.

The invention belongs to the field of medical laboratory apparatuses, and discloses a noninvasive fixer for a laboratory mouse. The noninvasive fixer is mainly characterized by structurally comprising a combined handle and clamping plates, wherein the plate surfaces of the clamping plates are mesh surfaces; a mouse mouth hole is formed in the mesh surface at one end of any clamping plate; a mouse mouth extension hole is formed outside the mouse mouth hole; and buckling parts are arranged on the handle. The conventional handholding mode is replaced, and the noninvasive fixer has the advantages of complete exposure of each part, fixing firmness, positioning accuracy, convenience for operation, adaptability to a plurality of experiments and capability of avoiding injuries to the laboratory mouse and an operator.

The invention discloses a laboratory mouse fixing device which comprises binding cloth and adhesive tapes, wherein the binding cloth is provided with adhesive positions corresponding to the abdomen and the neck of a laboratory mouse and provided with opening positions for enabling the head, the back, the abdomen and the four limbs of the laboratory mouse to be exposed out, and the adhesive tapes are arranged on the adhesive positions. The adhesive positions corresponding to the abdomen and the neck of the laboratory mouse fix the body and the neck of the laboratory mouse, the head, the back, the abdomen and the four limbs of the laboratory mouse can be exposed out from the opening positions, and the four limbs of the laboratory mouse miss step and have no point of strength, so that the binding effect is good. A laboratory technician can carry out experimental operation for the corresponding parts of the laboratory mouse through the openings of the binding cloth.

The invention provides a metabolic cage of laboratory mice. The metabolic cage of the laboratory mice is used for solving the problem that manure and urine of the laboratory mice can not be completely separated in the prior art. The metabolic cage of the laboratory mice comprises a support, a mouse cage, a separation collecting device, a conical separator, a urine collecting ring and an excrement tube. The mouse cage is fixed on the support, a mesh is arranged on the bottom of the mouse cage, and the separation collecting device is arranged below the mouse cage. The separation collecting device comprises a funnel, and the size of an upper opening of the separation collecting device is matched with the size of the mesh. The lower end of the conical separator is in an opening design. The conical separator is arranged inside the funnel and is coaxial with the funnel, the size of an upper opening of the conical separator is smaller than the size of a lower opening of the conical separator, and the size of the lower opening of the conical separator is larger than the size of the neck portion of the funnel. An annular urine collecting groove is formed in the upper surface of the urine collecting ring, and a lower opening of the funnel is placed in the urine collecting groove. The urine collecting ring is obliquely arranged, a urinary catheterization opening is formed in the lowest end of the urine collecting ring, and a urine tube used for containing urine is arranged below the urinary catheterization opening. The excrement tube is arranged below the funnel and used for containing excrement, a filtering membrane is arranged on the opening portion at the upper end of the urine tube, and the filtering membrane can be used for stopping and preserving the excrement so that the manure and urine separation effect can be made to be more obvious.

The invention discloses an experimental instrument, in particular to a laboratory mouse holder, comprising a first clamp arm, a second clamp arm, a first clamp sheet and a second clamp sheet. The first clamp arm is in crossing connection with the second clamp arm through a torsion spring. The torsion spring divides the first clamp arm into a first clamping end and a first handle. The torsion spring divides the second clamp arm into a second clamping end and a second handle. Both the first clamp sheet and the second clamp sheet are arc shaped. A first compression spring is disposed between a convex face of the first clamp sheet and the first clamping end. A second compression spring is disposed between a convex face of the second clamp sheet and the second clamping end. The first clamp sheet and the second clamp sheet are disposed between the first clamping end and the second clamping end. A concave face of the first clamp sheet faces a concave face of the second clamp sheet. The holder can replace a human hand to hold a laboratory mouse in a mouse cage, protecting a laboratory technician from being bitten. The holder is applicable to scientific research experiments, such as medical experiments, pharmaceutical experiments and biological experiments.

The invention provides a preparation method of a mice intestinal flora alteration ERIC-PCR (Enterobacterial Repetitive Intergenic Consensus sequence-based Polymerase Chain Reaction) fingerprint spectrum. The preparation method comprises the steps: moulding mice intestinal flora alteration caused by antibacterial-associated diarrhea, extracting a cecum content DNA, eliminating humic acid in the cecum content DNA, eliminating a PCR inhibitor in the cecum content DNA, performing ERIC-PCR amplification and native polyacrylamide gel electrophoresis (Native-PAGE), and comparing with a conventional flora analysis result so as to establish the ERIC-PCR fingerprint spectrum of the mice intestinal flora alteration. According to the preparation method, with a pure-series mice as a research object, the defect caused when a conventional bacterium culturing method is applied to the microflora analysis is solved. The preparation method can be used for rapid detection of the laboratory mice intestinal flora alteration, prevents the defects of complicated operation, long time consumption, large workload and the like of conventional bacterium separation and culturing, and is capable of accurately, rapidly and effectively detecting the mice intestinal flora distribution condition.

The invention belongs to the technical field of medicine, and relates to application of indolepopionic acid to preparation of drugs for preventing and / or treating tumor radiotherapy side effect. Through research, the fact that indolepopionic acid can obviously improve intestinal injury of mice, caused by gama ray irradiation, is found, the improvement shows that the survival rate of laboratory mice is obviously improved, the weight is risen again, the intestinal inflammation level is obviously reduced, and paradoxical reaction of the laboratory mice does not occur, the fact that indolepopionicacid can be effectively applied to the prevention and / or treatment of tumor radiotherapy side effect is shown, and the indolepopionic acid is low in toxic and side effect, and effective before and after being taken, and has a wide application potential.

The invention provides a tail loading device for swimming of a laboratory mouse. The tail loading device for the swimming of the laboratory mouse comprises an inner bushing and an outer bushing. The inner bushing is conical. The inner bushing is provided with a strip-shaped opening along the direction of the element of a cone, wherein the strip-shaped opening is communicated with two ports; a central angle which corresponds to the strip-shaped opening is smaller than 180 degrees; the inner bushing is provided with external threads; the inner surface of the outer bushing is in a truncated cone shape; and the inner surface of the outer bushing is provided with internal threads fitted with the external threads. The tail loading device for the swimming of the laboratory mouse provided by the invention is simple in structure and high in practicability, can be used for rapidly and effectively performing loading on the laboratory mouse, and moreover, the laboratory mouse cannot be hurt.

The invention relates to the fields of medical treatment evaluation and detection technology, and in particular relates to a method for preparing an intestinal mucosa injury animal model and applications of the prepared intestinal mucosa injury animal model. According to the scheme, the medicine L-MSO having good water solubility and realizing convenient and simple gavaging operation is adopted, the mouse intestinal mucosa injury is conveniently and stably induced, and meanwhile, the corresponding tissue pathological change and inflammatory factor change are caused. For the preparation method provided by the invention, the intestinal mucosa injury animal model is prepared from the metabolism injury direction obviously different from the molding direction of immune injury inducing in the prior art, particularly, the low-concentration L-MSO is adopted for preparing the common injured model, wherein the intestinal mucosa of the laboratory mouse has the mild-moderate injury, the high-concentration L-MSO is adopted for preparing the seriously injured model, wherein the intestinal mucosa of the laboratory mouse has the severe injury; the prepared intestinal mucosa injury model has the significant values for the research and practice of inflammatory bowel diseases.

The invention discloses a primer group, a kit and a method for detecting six mouse viruses by multiple immunity fluorescence. The primer group is designed by the aid of multiple RT-PCR (reverse transcription-polymerase chain reaction) technologies and Luminex liquid chip technologies and can simultaneously detect six high-infection viruses such as TMEV, MHV, MNV, Reo-3, MVM and MPV of mice. By the aid of the primer group, target amplification products are acquired through specific PCR, the amplification products, fluorescence encoding microspheres and streptavidin-phycoerythrin are hybridized, and different types of viruses are distinguished when a mean fluorescence intensity value is read by a Luminex detector. The method has the advantages of rapidness, high efficiency, high specificity and sensitivity, good repeatability and the like and can be applied to quality monitoring, epidemiological investigation and early warning of test mice. The primer group is good in flexibility, and varieties detecting the viruses can be increased and decreased as required on the basis.

The invention discloses a real-time tracing irradiation device for experiment mice. The real-time tracing irradiation device for experiment mice comprises the following steps of S1, obtaining an imageof a mouse to be irradiated, with a video camera, judging the current posture of the mouse, and determining the region of the head of the mouse in the image; S2, obtaining the image of the mouse after the position of the mouse is moved, and according to the region of the head of the mouse determined in the step 1, determining the region of the head of the mouse in the new image by a target tracing algorithm; S3, calculating the pixel coordinates of the head of the mouse, amending the pixel coordinates of the head of the mouse according to the current posture of the mouse, and then calculatingdisplacement vectors needing ray movement according to the position relationship of the video camera in a practical device and a ray irradiation direction based on the pixel coordinates; and S4, controlling rays to perform corresponding displacement and irradiate the head of the mouse according to the displacement vector in the step S3. According to the real-time tracing irradiation device for experiment mice disclosed by the invention, amendment is performed on the position according to the posture of the mouse, the ray emitting position is adjusted in a real-time manner so that rays move along with the movement of the mouse, and the problem that the mouse moving region is limited is solved.

The invention discloses a method for establishing an iron-overload mouse model corresponding to thalassemia Pesaro risk classification. The method comprises the following steps: injecting an iron-containing preparation to the abdominal cavity of a laboratory mouse, wherein the dosage is 1g / kg of the mouse, injecting the iron once a week, wherein the iron comprises Fe<2+> and Fe<3+>; establishing I-grade iron-overload mouse model in the Pesaro risk classification after the mouse is injected with the iron for one week; establishing II grade iron-overload mouse model in the Pesaro risk classification after the mouse is injected with the iron for 2-4 weeks; establishing III-grade iron-overload mouse model in the Pesaro risk classification after the mouse is injected with the iron for 5-8 weeks. The administration interval of the invention is in accordance with the pathological process of the hepatomegaly and hepatic fibrosis induced by the iron overload caused by repeated transfusion of the thalassemia patient, simultaneously, the mouse acute chalybeate poisoning death caused by the large-dose iron administration in a short time or the overlong mouse molding time caused by overlong iron administration time can be avoided.

The invention provides a preparation method for an anti-melamine monoclonal antibody and a kit for detecting melamine. The preparation method comprises the steps of injecting specific lymphocyte hybridoma cell strains of the melamine into an abdominal cavity of a laboratory mouse; taking ascites of the mouse; depositing the ascites through saturated ammonium sulfate and dialyzing after depositionis dissolved; purifying via affinity chromatography of Protein G; and collecting absorption peak to finally obtain the anti-melamine monoclonal antibody. The anti-melamine monoclonal antibody prepared by the method can be used for producing competition enzyme linked immunity detection reagent kits and colloidal gold test strips to rapidly detect residues of the melamine in food, thereby meeting ademand for rapidly detection of a large quantity of samples.

The invention relates to an automatic tracking method of temperature place preference behaviors in laboratory mice. The method at least includes the steps: a laboratory mice temperature stimulation passage is divided into different temperature zones, each temperature zone is provided with a heating plate and a heating plate temperature sensor, each temperature zone is provided with temperature zone boundary left and right sensor bars, the heating plates are electrically connected with a processing unit through a heating control circuit, the processing unit is respectively connected with the heating plate temperature sensors, the boundary right sensor bars and the boundary left sensor bars through signal wires, the processing unit sets certain temperature spots for the different temperature zones through a human-machine interface, the processing unit controls heating through the heating control circuit, the temperatures of the corresponding temperature zones are controlled in the required temperature field accordingly, the temperature zone boundary of the different temperature zones is used for distinguishing a passing direction of the laboratory mouse through the boundary right sensor bars and the boundary left sensor bars, and the durations of the laboratory mouse being in the temperature zones at different times are provided according to recorded durations, and temperature preference behaviors of the laboratory mice are further understood.

The invention relates to construction for a number 1 chromosome substitution laboratory mouse strain C57BL / 6-Chr1NZW. According to the construction for the number 1 chromosome substitution laboratory mouse strain C57BL / 6-Chr1NZW, a new chromosome substitution laboratory mouse strain is constructed. A genetic background of the chromosome substitution laboratory mouse strain is based on a C57BL / 6 mouse, while wherein a number 1 chromosome is from an NZW mouse. According to the construction for the number 1 chromosome substitution laboratory mouse strain C57BL / 6-Chr1NZW, the chromosome substitution laboratory mouse strain can be used for research of character differences brought by different number 1 chromosome genomes under the same genome background, and can be a useful tool for research of the chromosome genome functions by adoption of a genetic method.

The invention discloses a sex identification and sorting system for rats and mice. The system comprises an expelling platform for expelling the rats and mice in cages on the expelling platform, a laboratory mouse classification slideway connected with the expelling platform and used for sliding the rats and mice downwards and separating the rats and mice into independent individuals, a linear array industrial camera located below the laboratory mouse classification slideway and used for shooting abdominal images of the rats and mice, calling an image processing special algorithm to calculate the distance between genitals and anuses of the rats and mice, and judging the genders and body sizes of the rats and mice according to the distance; and a laboratory mouse pipeline connected with thelaboratory mouse classification slideway and used for feeding the rats and mice with the different genders and sizes into a laboratory mouse sorting area. According to the sex identification and sorting system, sex distinguishing can be conducted on a large batch of sexually mature experimental rats and mice, the rats and mice are sub-packaged in different areas according to different sexes, sex classification of the experimental rats is efficiently and accurately achieved, a large amount of time is saved, and pollution caused by external factors to the rats and mice is greatly reduced.

The invention discloses feed for gene editing laboratory mice. The feed is prepared from components in parts by weight as follows: 30-35 parts of corn, 10-15 parts of wheat, 15-20 parts of soybean meal, 5-12 parts of broken rice, 3-10 parts of fish meal, 1-5 parts of oil, 2-9 parts of a yeast hydrolysate, 0.8-1.2parts of stone powder, 1-2 parts of calcium hydrogen phosphate, 0.5-1 part of compound minerals, 0.1-0.5 parts of decavitamin, 0.1-0.2 parts of methionine and 0.1-0.2 parts of lysine. The feed has balanced nutrition and good palatability and can increase the feed intake of the gene editing laboratory mice, promote growth and breeding and significantly increase the survival rate of the gene editing laboratory mice, the nutrition index of the feed meets the nutrition index of national standard GB14924.3-2010 of Complete Nutritional Feed for Laboratory Animals, the hygienic index meets the hygienic index requirements of national standard GB14924.1-2010 of Complete Nutritional Feed for Laboratory Animals, and the feed for the gene editing laboratory mice has bright application prospects.

The invention relates to the field of laboratory mouse motion recognition, and discloses a laboratory mouse sniffing motion recognition method, module and system, and the method comprises the steps: collecting laboratory mouse video data; determining key points of the shape and posture of the laboratory mouse, and constructing a key point model; generating a key point identification module according to the experimental mouse video data and the key point model; and using the key point identification module to identify the sniffing action of the laboratory mouse. The identification module comprises a video acquisition sub-module, a key point model construction sub-module and a key point identification module generation sub-module. The identification system comprises a box body, a diffuse reflection plate, a lining plate, an illumination device and an identification module. According to the invention, the sniffing action of the laboratory mouse is identified based on the attitude data ofthe laboratory mouse, so that the sniffing action and attitude change of the laboratory mouse can be accurately and efficiently identified in real time; shadow-free laboratory mouse video data can becollected, accurate video data collection is guaranteed, and automatic recognition of laboratory mouse sniffing actions is achieved.

A clip is described for the purposes of aiding researchers with tail vessel injections in laboratory mice. The clip, consisting of a tourniquet with an attached ring and platform, allows the researchers to place the tourniquet over the tail of the mouse, either by hand or using a placement tool, and subsequently insert the specified needle through the ring, where it traverses at the appropriate angle to successfully puncture and enter the vessel lumen. The disposable nature of the clip allows researchers to maintain financial goals and decrease sterility issues. Additional features could be added to the clip for either practical or personal reasons. The clip is where cost-effectiveness and efficacy combine to provide the user with a satisfactory aid in the field of vessel injection systems.

The invention discloses an awake laboratory mouse bladder pressure measuring catheter suite which comprises a bladder cannula and a lengthening component. A guide wire is arranged inside the bladder cannula. The front end of the guide wire is designed to be a conic sharp end and the rear end of the bladder cannula is provided with a closing clamp. The lengthening component comprises a PE-50 catheter, a double-head connecting tube and a three-way catheter. One end of the PE-50 catheter is connected with the double-head connecting tube and the other end of the PE-50 catheter is connected with one catheter opening of the three-way catheter. The double-head connecting tube is connected with the rear end of the bladder cannula and fixed through the closing clamp. The awake laboratory mouse bladder pressure measuring catheter suite has the advantages that the inserting end of the bladder cannula can be fixed through an automatic curling structure generated after the guide wire of the inserting end of the bladder cannula is extracted, and the detection success rate and detection result accuracy are improved; by the use of the suite, operation difficulty is greatly lowered, experiment efficiency is improved, and damage to the skin and the bladder wall of an experiment mouse is reduced.

The invention relates to construction for a number 1 chromosome substitution laboratory mouse strain C57BL / 6-Chr1C3H / HeJ. According to the construction for the number 1 chromosome substitution laboratory mouse strain C57BL / 6-Chr1C3H / HeJ, a new chromosome substitution laboratory mouse strain is constructed. A genetic background of the chromosome substitution laboratory mouse strain is based on a C57BL / 6 mouse, while wherein a number 1 chromosome is from a C3H / HeJ mouse. According to the construction for the number 1 chromosome substitution laboratory mouse strain C57BL / 6-Chr1C3H / HeJ, the chromosome substitution laboratory mouse strain can be used for research of character differences brought by different number 1 chromosome genomes under the same genome background, and can be a useful tool for research of the chromosome genome functions by adoption of a genetic method.

The invention discloses an automatic pregnant mother mouse breeding method. The automatic pregnant mother mouse breeding method comprises the steps that an RFID is arranged on each cage, an AGV carrying robot takes down the corresponding cage from a goods shelf and transports the cage to a mouse changing workbench, the cage with death of a mother mouse is processed in time, the pregnancy conditionof the mother mouse is monitored, if a baby mouse is born, an RFID reader-writer resets RFID storage information on the cage, and the cage is conveyed to a baby mouse sex identification and sorting workshop through a reproductive processing channel. The mother mouse breeding method is applied to production activities of large-scale and automatic breeding of rats and baby mice by experimental animal breeding units in a barrier environment, and the quality requirements of client groups such as scientific research institutions, colleges and universities, hospitals and pharmaceutical factories onthe experimental rats and baby mice are met. Image processing, automatic cage changing and other technologies are applied to the automatic breeding process of the pregnant mother mouse, interferencefactors in the artificial breeding process are reduced, stimulation of the outside to laboratory mouse breeding is reduced, and laboratory young mice with better quality can be bred.

The invention relates to a method for inducing a chronic and acute liver failure mouse model, in particular to a method for inducing the chronic and acute liver failure mouse model by using carbon tetrachloride, which comprises the following steps of: a grouping method is characterized by randomly and averagely dividing Balb / c inbred line mice into a model group and a control group; an administration method is characterized by diluting carbon tetrachloride to concentrations of 10% and 50% by using olive oil, continuously injecting 10% carbon tetrachloride into the abdominal cavity of a mouse in the model group according to 5-8ml / kg body weight, administrating twice a week, and continuously administrating for 8 weeks; after 8 weeks, injecting the 50% carbon tetrachloride into the abdominalcavity once according to 5-8 ml / kg body weight; and a detection method is characterized by performing biochemical index detection and liver pathological examination on the experimental mouse. The method has the beneficial effects that Balb / c inbred line mice are adopted as model animals, most inbred lines can be adopted, the repeatability is better, the cost is lower compared with rats, and the operation is simpler and more convenient. The model better conforms to the disease development process of clinical hepatitis B virus (HBV) related chronic and acute liver failure.

The invention relates to the field of biological medicine, in particular to an application of poly inosine-cytidine, imiquimod and gardiquimod in constructing a virus immunity mouse model. The inventor of the invention employs the mouse model and forms an antivirus immune state by using poly inosine-cytidine, imiquimod and gardiquimod; an experiment mouse administered with poly inosine-cytidine is found to appear lung bacterium clearance impairment; in addition, poly inosine-cytidine is found to be capable of mediating IFNI expression; and finally, IFNI can induce a defense mechanism of a lung against gram positive pathogenic bacteria.