126 results about "Pox viruses" patented technology

The present invention concerns methods and compositions for the treatment of cancer and cancer cells using altered poxviruses, including a vaccinia virus that has been altered to generate a more effective therapeutic agent. Such poxviruses are engineered to be attenuated or weakened in their ability to affect normal cells. In some embodiments, methods and compositions involve poxviruses that possess mutations that result in poxviruses with diminished or eliminated capability to implement an antiviral response in a host. Poxviruses with these mutations in combination with other mutations can be employed for more effective treatment of cancer.

The present invention relates to a fowlpox virus genome which has modifications in one or more wild-type FPV genes. The present invention also relates to a viral particle comprising such a genome and its use to deliver a nucleotide of interest (NOI) to a target cell. The present invention also relates to vaccination methods, particularly a method which comprises administering a priming composition (which comprises a first non-replicating viral vector) and a boosting composition (which comprises a second non-replicating viral vector) to a subject to treat and/or prevent a disease.

The present invention relates to novel plasmid constructs useful for the delivery of DNA vaccines. The present invention provides novel plasmids having a transcription cassette capable of directing the expression of a vaccine nucleic acid insert encoding immunogens derived from any pathogen, including fungi, bacteria and viruses. The present invention, however, is particularly useful for inducing in a patient an immune response against pathogenic viruses such as HIV, measles or influenza. Immunodeficiency virus vaccine inserts of the present invention express non-infectious HIV virus-like particles (VLP) bearing multiple viral epitopes. VLPs allow presentation of the epitopes to multiple histocompatability types, thereby reducing the possibility of the targeted virus escaping the immune response. Also described are methods for immunizing a patient by delivery of a novel plasmid of the present invention to the patient for expression of the vaccine insert therein. Optionally, the immunization protocol may include a booster vaccination that may be a live vector vaccine such as a recombinant pox virus or modified vaccinia Arbora vector. The booster live vaccine vector includes a transcription cassette expressing the same vaccine insert as the primary immunizing vector.

The present invention relates to highly attenuated animal smallpox viral strains and to the use thereof as paramunity inducers or for producing vector vaccines. As a result of the high attenuation process, the claimed animal smallpox strains lose their virulent and immunising properties. The invention also relates to a method for producing such highly attenuated pox virus strains and the use thereof for inducing paramunity, i.e. for activating the non-specific immune system in mammals and humans or for producing vector vaccines for specific immunisation with the positive side-effect of paramunisation. The claimed highly attenuated animal smallpox viruses are thus suitable for preventing and treating diseases associated with an immune deficiency. Preferred embodiments relate to highly attenuated orthopox—(e.g. camel smallpox viruses), leporipox—(e.g. myxoma viruses), avipox-, parapox- and other orthopox viral strains, such as MVA, which have excellent paramunisation properties and in which the immunising properties have been lost.

The invention relates to a colloidal gold test strip based on a goat pox virus. The colloidal gold test strip comprises a PVC (Polyvinyl Chloride) rubber plate, a nitrocellulose membrane, a colloidal gold mat, a sample mat and absorbent paper, wherein the sample mat adheres to one end of the PVC rubber plate; the absorbent paper adheres to the other end of the PVC rubber plate; the colloidal gold mat and nitrocellulose membrane adhere to the middle part of the PVC rubber plate in sequence; the sample mat adheres to one end of the golden standard mat; the nitrocellulose membrane adheres to the other end of the colloidal gold mat; the nitrocellulose membrane adheres to the absorbent paper; the colloidal gold mat is coated with an anti-GTPV-P32 protein monoclonal antibody marked with colloidal gold; the nitrocellulose membrane is provided with a detection line which is linearly coated with an anti-GTPV-ORF122 protein monoclonal antibody at the concentration of 1mg/mL, and a quality control line which is linearly coated with a goat anti-mouse IgG (Intravenous Gamma Globulin) antibody at the concentration of 1mg/mL in sequence along the flow direction of a sample. Meanwhile, the invention further discloses a preparation method of the diagnosis strip. The test strip has the characteristics of high specificity, high stability, easiness in operation and rapid detection, and is suitable for field detection at the occurrence of animal epidemic diseases.

The invention discloses a real-time fluorescent RPA kit for rapidly detecting capripoxvirus and application thereof, and further discloses a test strip RPA kit for rapidly detecting the capripoxvirus and application thereof. The two kits comprise primers shown as SEQ ID NO.1 and SEQ ID NO.2 respectively and respective probes, and modified bases and detection platforms of the primers and the probes are different although the primers and the probes aim at a same target sequence. Experiments prove that the sensibility of the real-time fluorescent RPA kit and the sensibility of the test strip RPA kit are both 102 copies per response, and both of the two kits only can specially detect a sheep pox virus and a goat pox virus. A capripoxvirus suspected sample is detected by the two kits separately, and results show that the detection results of the two kits both have the very high conformity with that of qPCR. Therefore, the two kits both can rapidly, efficiently and sensitively detect the sheep pox virus or the goat pox virus, and an effective technological means is supplied to differentiation and diagnosis of the sheep pox virus or the goat pox virus.

The invention discloses a goat pox virus and sheep pox virus dual-PCR (Polymerase Chain Reaction) detection kit and a dual-PCR method for rapidly identifying and detecting goat pox virus and sheep pox virus. One pair of PCR primers is respectively designed according to genome sequences of the goat pox virus and the sheep pox virus, and corresponding specific fragments can be obtained through amplification, thus detection and identification of the goat pox virus and the sheep pox virus are realized. By using the dual-PCR technology, the goat pox virus and the sheep pox virus can be simultaneously detected by using a single tube, thus detection cost and workload of goat pox and sheep pox are reduced, and identification detection of the goat pox and the sheep pox is realized. According to the invention, a dual-PCR method with the advantages of strong specificity, high sensitivity, time and labor saving and easiness in observing results is established, and the advantages of convenience for operation, strong specificity, high sensitivity, high repeatability and no complex post-treatment are achieved.

The invention discloses a pigeon pox resisting Chinese medicinal composition, which comprises the following components in portion by weight: 25 to 45 portions of root of kudzuvine, 25 to 45 portions of forsythia, 20 to 35 portions of cimicifuga foetida, 20 to 35 portions of glabrous greenbrier rhizome, 20 to 35 portions of dianthus superbus, 15 to 30 portions of honeysuckle, and 15 to 30 portions of liquorice. The invention also discloses a method for preparing oral liquid of the Chinese medicinal composition for resisting the pigeon pox. The Chinese medicament replaced for western medicine is used for treating virus infection of the pigeon pox, and can reduce or eliminate medicament residues in poultry and eggs so that the Chinese medicinal composition is favorable for pollution-free production of animal products, and can be widely applied to pox virus infection of various birds comprising pigeons; after the pigeons suffer from the pox virus infection, the product can treat, so remarkable curative effect can be obtained, and the healing rate reaches more than 95 percent; and in particular for the produced smallpox scabs, the smallpox scabs can fall and heal after medication for one week and do not relapse.

Disclosed are methods of treating, inhibiting, or preventing a viral infection in a mammal in need thereof by administering a therapeutically or prophylactically effective amount of an inhibitor of FAP, an inhibitor of DPPIV, an inhibitor of DPP8, or an inhibitor of DPP9. The inhibitor may act as both an inhibitor of DPPIV and an inhibitor of DPP8/9. The viral infection includes, but is not limited to, hepatitis B virus, hepatitis C virus, human immunodeficiency virus, Polio virus, Coxsackie A virus, Coxsackie B virus, Rhino virus, respiratory syncytial virus, dengue virus, equine infectious anemia virus, Echo virus, small pox virus, Ebola virus, and West Nile virus.

The invention provides a detection method used for detecting sheep pox virus, wherein real-time fluorescent quantitative PCR (polymerase chain reaction) detection is carried out on a DNA (deoxyribose nucleic acid) template of a sample by designing a pair of primers and one probe; and the invention also provides a kit used for detecting the sheep pox virus by applying the detection method. The kit used for detecting the sheep pox virus designs two specific primers aiming at P32 and one probe, real-time quantitative PCR detection is carried out on sheep pox virus Taqman, and a real-time quantitative method established aiming at the gene has high specificity and good stability; the kit used for detecting the sheep pox virus is easy to operate, a user only needs to add DNA of a sample to be inspected into a reaction tube, a detection result can be obtained according to an amplification curve, time and labour are saved, cost is lower, and time is shortened to 1-2 hours from the original 3-4 hours; and the kit used for detecting the sheep pox virus has higher sensitivity compared with common PCR and can be used for detecting the sheep pox virus with low content.

The invention discloses primers, a kit and a detection method for detecting plum pox virus based on LAMP technology. The primers include: forward outer primer F3: 5'-CCTTTGATTTCTACGAGATGAC-3'; reverse outer primer B3: 5'-ACCACTACACTCCCCTC-3 '; forward internal primer FIP: 5'-TCCAAGCCAAATAAACGATTTTGAAGTGAGGCACATATTCAAATGAAGG-3'; wherein said forward internal primer FIP comprises F1c and F2; reverse internal primer BIP, wherein said reverse internal primer BIP comprises B1c and B2; loop Primers: LF: 5'-CATTTCTCAATGCTGCTG-3'; LB: 5'-ACCGCTGGTGATGTTAA-3'. The purpose of the present invention is to overcome the deficiencies in the prior art, to provide a quick, easy, efficient, high accuracy, strong specificity, high sensitivity, primers, kits, Method for detection of plum pox virus.

The invention discloses a primer, a probe and a method for detecting knopvelsiekte virus by a RAA fluorescence method. The primer and the probe are suitable for the detection by the RAA fluorescence method, can accurately detect knopvelsiekte virus plasmids without cross reaction with mycoplasma, bovine infectious rhinotracheitis virus, bovine viral diarrhea virus, bovine parainfluenza virus, bovine respiratory syncytial virus, goat pox virus and sheep pox virus, and have a specificity of 100%. The method is fast and easy to achieve high throughput, and can reduce time and cost for detection.The method for rapid detection of DNA of the knopvelsiekte virus based on the RAA fluorescence method has high sensitivity reaching 10 copies / reaction.

The invention discloses a multiplex real-time fluorescent polymerase chain reaction (PCR) primer for simultaneously detecting mouse adenovirus and poxvirus muris mouse pox virus and a probe, which respectively contain oligonucleotides shown by SEQ ID NO: 1 to 6 in a sequence table. A multiplex real-time detection reagent kit containing the primer and the probe can be used for one-step combined detection of the mouse adenovirus and the poxvirus muris mouse pox virus, has the advantages of high speed, simplicity, high sensitivity, high specificity and high recovery rate, solves the problems of complexity, time waste, long period and certain requirement on an operation laboratory of the existing detection measure and has good application prospects.

The invention discloses a lamb testis support cell immortalized cell line and an establishment method and application thereof. The lamb testis support cell immortalized cell line is named a lamb testis support cell immortalized cell line hTERT-LSC with a collection number CCTCC NO: C2018202. Tests find that the hTERT-LSC cell line can realize continuous passage for more than 60 generations, and the growth and proliferation characteristics are not changed. The cell line is relatively sensitive to inoculation of the sheep pox virus, can efficiently proliferate the sheep pox virus, and can ensurethe uniformity and stability of the virus. The titer of the sheep pox virus proliferated by the cell line is equivalent to that of the lamb testis support cell LSC, and the cell division rate is higher than that of the LSC and can reach 1:3. Furthermore, by utilizing the immortalized cell line for preparing a vaccine, the production process is simplified, the production cycle is shortened, the production cost is reduced, and simultaneously, the quality stability of a prepared sheep pox virus vaccine is also ensured. Therefore, the invention provides a new technical means for large-scale production of the sheep pox virus vaccine.

The present invention is directed to a dry suspension for reconstitution containing Tecovirimat (ST-246) powder and simethicone. The dry suspension is dispersed in water to provide an aqueous pharmaceutical suspension formulation for oral administration for treating orthopoxvirus infections and/or eczema vaccinatum. The suspension formulation exhibits excellent stability and good dissolution and has an improved taste and texture.

The invention relates to goat pox virus/contagious pustular dermatitis virus multiplex PCR (polymerase chain reaction) detection primers of which the sequences are disclosed as SEQ ID No.1-4. The 2 pairs of primers are utilized to carry out PCR amplification on the corresponding target genes so as to establish a multiplex PCR detection method; and the primers can be used for quickly identifying goat pox virus and contagious pustular dermatitis virus in one reaction system, and can simultaneously determine whether two-virus mixed infection occurs, thereby having important application value in the aspects of clinical sample quick screening, disease quick diagnosis and the like.

The invention discloses a kit and method for detecting plum pox virus by applying a nano magnetic bead. The kit contains primers PPV1, namely 5'-ACCGAGACCACTACACTCCC-3', and PPV2, namely 5'-CAGACTACAGCCTCGCCAGA-3'. The method for detecting the plum pox virus by applying the nano magnetic bead is characterized by comprising the following steps: 1) preparing a sample; 2) cleaning the nano magnetic bead; 3) combining; 4) cleaning; 5) carrying out splitting decomposition; 6) sampling; 7) carrying out RT-PCR amplification, and after reaction is finished, taking 5mu L reaction product for carrying out electrophoretic analysis on 1% agarose gel prepared by adopting 1*TAE buffer solution. The invention aims at overcoming the defects of the prior art and provides the kit and the simple method for detecting the plum pox virus by applying the nano magnetic bead.

The avian infectious laryngotracheitis virus and fowl pox virus are cultured by using chicken whole embryo fibroblasts. Particularly, the avian infectious laryngotracheitis virus is successfully cultured by using the chicken whole embryo fibroblasts, the avian infectious laryngotracheitis virus and the fowl pox virus cultured by using the chicken whole embryo fibroblasts are obtained, and a combined freeze-drying active vaccine after a proper freeze-drying protectant is added. The combined freeze-drying active vaccine can be inoculated for immunization in a subalar and subcutaneous inoculation manner, so that side effects of the avian infectious laryngotracheitis virus can be alleviated, immune operations can be simplified, stress response can be reduced and production cost can be lowered. At the same time, the freeze-drying protectant prepared by a novel formula can effectively prolong storage time of the active vaccine and provide convenience for storing and transporting the products.

Disclosed herein are methods and compositions related to the treatment, prevention, and/or amelioration of cancer in a subject in need thereof. In particular aspects, the present technology relates tothe use of poxviruses, including a recombinant modified vaccinia Ankara (MVA) virus or vaccinia virus with deletion of vaccinia host-range factor C7 (MVA[delta]C7L and VACV[delta]C7L, respectively),alone or in combination with immune checkpoint blocking agents, as an oncolytic and immunotherapeutic composition. In some embodiments, the technology of the present disclosure relates to a MVA[delta]C7L or VACV[delta]C7L virus further modified to express human Fms-like tyrosine kinase 3 ligand (Flt3L).