The invention discloses a blocking ELISA kit for detecting a bovine parainfluenza virus type 3 antibody. The kit comprises a pre-coated elisa plate and an enzyme-labeled antibody, the pre-coated elisaplate is an elisa plate coated with purified goat parainfluenza virus type 3; the enzyme labeled antibody is a monoclonal antibody 2E6 labeled by horse radish peroxidase; the monoclonal antibody 2E6is secreted by a hybridoma cell strain 2E6; the hybridoma cell strain 2E6 is preserved in China Center for Type Culture collecting on February 1, 2016, the preservation address is Wuhan University, Wuhan, China, the preservation number is CCTCC NO: C201627, and the hybridoma cell strain 2E6 is classified and named as hybridoma cell strain 2E6. The kit disclosed by the invention is good in specificity, high in sensitivity, good in stability, capable of rapidly, simply, conveniently and efficiently detecting the bovine parainfluenza virus type 3 antibody, low in cost and suitable for high-throughput detection of samples.