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974 results about "Multiplex polymerase chain reaction" patented technology
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Multiplex polymerase chain reaction (Multiplex PCR) refers to the use of polymerase chain reaction to amplify several different DNA sequences simultaneously (as if performing many separate PCR reactions all together in one reaction). This process amplifies DNA in samples using multiple primers and a temperature-mediated DNA polymerase in a thermal cycler. The primer design for all primers pairs has to be optimized so that all primer pairs can work at the same annealing temperature during PCR.
Method and devices for extremely fast DNA replication by polymerase chain reactions (PCR)
InactiveUS6180372B1Shorten cycle timeHigh sensitivityBioreactor/fermenter combinationsNanotechPolymerase LBiological materials
The invention concerns methods and instruments for fast, selective replication of deoxyribonucleic acid (DNA) from biomaterial through the known polymerase chain reaction (PCR), working in individual duplication thermocycles. The invention consists of extremely brief cycle times of only a few seconds for the PCR reactions, generated, on the one hand, by reaction chambers for the reception of the reaction solution constructed of a pattern of fine capillaries in close proximity to heating and cooling elements in order to optimally accelerate the temperature setting in the reaction solution for the three temperature phases of the PCR duplication cycles and, on the other hand, by keeping the flow rates in the capillaries to a minimum during the amplification phase so that the polymerase reaction is not disturbed. The capillary pattern can be simply produced by means of microsystern technology.
Owner:BRUKER FRANZEN ANALYTIK
Polymerase chain reaction of DNA of which base sequence is completely unidentified
The present invention relates to a process for amplifying DNA of an organism. More particularly, the present invention is directed to a process for amplifying DNA of an organism through Polymerase Chain Reaction(PCR) without any information regarding a primer needed for amplifying DNA of an organism.
Owner:BIONEER
Fluorometer with low heat-generating light source
InactiveUS6852986B1Avoid spreadingHeating or cooling apparatusPhotometryThermal cyclerLight-emitting diode
This invention concerns a fluorometer preferably combined with a thermal cycler useful in biochemical protocols such as polymerase chain reaction (PCR) and DNA melting curve analysis. The present flourometer features a low heat-generating light source such as a light emitting diode (LED), having a one-to-one correspondence to each of a plurality of sample containers, such as capped PCR tubes in a standard titer tray. The flourometer of the present invention further comprises an optical path between each LED and its correspondingly positioned container, and another optical path between each flourescing sample within the positioned container and an optical signal sensing means. The instrument can be computer controlled.
Owner:QUALICON DIAGNOSTICS LLC
Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions
InactiveUS7097980B2Maximize productionLess primerMicrobiological testing/measurementRecombinant DNA-technologyNucleotideCoupling
The present invention relates to the detection of nucleic acid sequence differences using coupled ligase detection reaction and polymerase chain reaction. One aspect of the present invention involves use of a ligase detection reaction coupled to a polymerase chain reaction. Another aspect of the present invention relates to the use of a primary polymerase chain reaction coupled to a secondary polymerase chain reaction coupled to a ligase detection reaction. A third aspect of the present invention involves a primary polymerase chain reaction coupled to a secondary polymerase chain reaction. Such coupling of the ligase detection reaction and the polymerase chain reaction permits multiplex detection of nucleic acid sequence differences.
Owner:CORNELL RES FOUNDATION INC
Coupled polymerase chain reaction-restriction-endonuclease digestion-ligase detection reaction process
InactiveUS7014994B1Sensitive highOptimizationSugar derivativesMicrobiological testing/measurementNucleotideWild type
The present invention provides a method for identifying one or more low abundance sequences differing by one or more single-base changes, insertions, or deletions, from a high abundance sequence in a plurality of target nucleotide sequences. The high abundance wild-type sequence is selectively removed using high fidelity polymerase chain reaction analog conversion, facilitated by optimal buffer conditions, to create a restriction endonuclease site in the high abundance wild-type gene, but not in the low abundance mutant gene. This allows for digestion of the high abundance DNA. Subsequently the low abundant mutant DNA is amplified and detected by the ligase detection reaction assay. The present invention also relates to a kit for carrying out this procedure.
Owner:LOUISIANA STATE UNIV +1
Personalized Tumor Biomarkers
ActiveUS20150344970A1Microbiological testing/measurementLibrary member identificationBlood plasmaWilms' tumor
Clinical management of human cancer is dependent on the accurate monitoring of residual and recurrent tumors. We have developed a method, called personalized analysis of rearranged ends (PARE), which can identify translocations in solid tumors. Analysis of four colorectal and two breast cancers revealed an average of nine rearranged sequences (range 4 to 15) per tumor. Polymerase chain reaction with primers spanning the breakpoints were able to detect mutant DNA molecules present at levels lower than 0.001% and readily identified mutated circulating DNA in patient plasma samples. This approach provides an exquisitely sensitive and broadly applicable approach for the development of personalized biomarkers to enhance the clinical management of cancer patients.
Owner:THE JOHN HOPKINS UNIV SCHOOL OF MEDICINE
Dual phase multiplex polymerase chain reaction
ActiveUS7432055B2Further amplificationMicrobiological testing/measurementLibrary member identificationGenomic DNASingle pair
Highly specific and sensitive methods were developed for multiplex amplification of nucleic acids on supports such as microarrays. Based on a specific primer design, methods include five types of amplification that proceed in a reaction chamber simultaneously. These relate to four types of multiplex amplification of a target DNA on a solid support, directed by forward and reverse complex primers immobilized to the support and a fifth type—pseudo-monoplex polymerase chain reaction (PCR) of multiple targets in solution, directed by a single pair of unbound universal primers. The addition of the universal primers in the reaction mixture increases the yield over the traditional “bridge” amplification on a solid support by approximately ten times. Methods that provide multitarget amplification and detection of as little as 0.45-4.5×10−12 g (equivalent to 102-103 genomes) of a bacterial genomic DNA are disclosed.
Owner:UCHICAGO ARGONNE LLC +1
DNA amplification of a single cell
InactiveUS6673541B1Improve accuracyImprove comprehensive applicabilitySugar derivativesMicrobiological testing/measurementSingle-strand conformation polymorphismComparative genomic hybridization
The present invention relates to a novel method for the amplification of DNA, this method being particularly useful for the amplification of the DNA or the whole genome of a single cell, chromosomes or fragments thereof. Described is also the use of the method in DNA analysis for medical, forensic, diagnostic or scientific purposes, like comparative genomic hybridization (CGH)-, fluorescence in situ hybridization (FISH)-, polymerase chain reaction (PCR)-, single strand conformation polymorphism (SSCP)-, DNA sequence-, "loss of heterozygosity" (LOH)-, fingerprint- and / or restriction fragment length polymorphism (RFLP)-analysis.
Owner:AMGEN RES (MUNICH) GMBH
Method for detection of promoter methylation status
InactiveUS7358048B2Precise “ molecular signature ”Accurately methylation statusMicrobiological testing/measurementFermentationCapillary electrophoresisElectrophoresis
The present invention relates to the detection of promoter methylation status using a combination of either modification of methylated DNA or restriction endonuclease digestion, multiplex polymerase chain reaction, ligase detection reaction, and a universal array or capillary electrophoresis detection.
Owner:CORNELL RES FOUNDATION INC
Dual phase multiplex polymerase chain reaction
ActiveUS20050196760A1Increase volumeFurther amplificationMicrobiological testing/measurementLibrary member identificationGenomic DNASingle pair
Highly specific and sensitive methods were developed for multiplex amplification of nucleic acids on supports such as microarrays. Based on a specific primer design, methods include five types of amplification that proceed in a reaction chamber simultaneously. These relate to four types of multiplex amplification of a target DNA on a solid support, directed by forward and reverse complex primers immobilized to the support and a fifth type—pseudo-monoplex polymerase chain reaction (PCR) of multiple targets in solution, directed by a single pair of unbound universal primers. The addition of the universal primers in the reaction mixture increases the yield over the traditional “bridge” amplification on a solid support by approximately ten times. Methods that provide multitarget amplification and detection of as little as 0.45-4.5×10−12 g (equivalent to 102-103 genomes) of a bacterial genomic DNA are disclosed.
Owner:UCHICAGO ARGONNE LLC +1
Reaction buffer composition for nucleic acid replication with packed DNA polymerases
The invention relates to compositions, methods, and kits for nucleic acid replication, including polymerase chain reaction (PCR) and mutagenesis reactions. A buffer composition is provided which allows higher concentrations of DNA polymerase to be used, resulting in greater yield of amplified product and faster reaction kinetics.
Owner:AGILENT TECH INC
Amplicon rescue multiplex polymerase chain reaction for amplification of multiple targets
Disclosed is a method for amplifying and detecting polynucleotides which can provide sensitive, specific detection of multiple targets from a clinical specimen within a relatively short time.
Owner:CB BIOTECHNOLOGIES INC +1
DNA index library building method based on high throughput sequencing
ActiveCN102409048AImprove production efficiencyHigh sequencing throughputNucleotide librariesMicrobiological testing/measurementSingle sampleA-DNA
The invention provides unique index sequences with a length of 7bp. The index sequences can be respectively imported into a DNA(deoxyribonucleic acid) index library through adapter link and PCR (polymerase chain reaction). The invention provides a method for building the DNA index library by using the index sequences based on a solexa sequencing platform of the current illumina company, and the method is applied to solexa DNA sequencing and has the effects on improving the preparation efficiency of the DNA index library, increasing the sequencing throughput of the DNA samples and lowering thesolexa sequencing cost of a single sample.
Owner:BGI TECH SOLUTIONS
Primer set for detecting functional genes of wheat on basis of KASP [competitive allele specific PCR (polymerase chain reaction)] technology and application of set primer
ActiveCN105112546AImprove throughputImprove detection efficiencyMicrobiological testing/measurementDNA/RNA fragmentationAgricultural scienceNucleotide sequencing
The invention discloses a primer set for detecting functional genes of wheat on the basis of a KASP [competitive allele specific PCR (polymerase chain reaction)] technology and application of the set primer. The primer set comprises totally 14 groups of KASP primers. The KASP primers are respectively designed for the 14 functional genes of the wheat, and particular nucleotide sequences of the KASP primers are sequences 1-42 in sequence tables. The primer set and the application have the advantages that the functional genes of different wheat varieties can be quickly detected by the aid of the KASP primer set, methods for detecting the functional genes of the wheat are simple, convenient and speedy, and detection results are accurate and reliable; the primer set has an important theoretical significance and important economic value in assistant selection of the wheat varieties by the aid of molecular markers.
![Primer set for detecting functional genes of wheat on basis of KASP [competitive allele specific PCR (polymerase chain reaction)] technology and application of set primer](https://images-eureka.patsnap.com/patent_img/935db461-2e79-4d64-a703-4ba19d999536/HDA0000809386820000011.PNG "Primer set for detecting functional genes of wheat on basis of KASP [competitive allele specific PCR (polymerase chain reaction)] technology and application of set primer")
![Primer set for detecting functional genes of wheat on basis of KASP [competitive allele specific PCR (polymerase chain reaction)] technology and application of set primer](https://images-eureka.patsnap.com/patent_img/935db461-2e79-4d64-a703-4ba19d999536/HDA0000809386820000012.PNG "Primer set for detecting functional genes of wheat on basis of KASP [competitive allele specific PCR (polymerase chain reaction)] technology and application of set primer")
![Primer set for detecting functional genes of wheat on basis of KASP [competitive allele specific PCR (polymerase chain reaction)] technology and application of set primer](https://images-eureka.patsnap.com/patent_img/935db461-2e79-4d64-a703-4ba19d999536/HDA0000809386820000013.PNG "Primer set for detecting functional genes of wheat on basis of KASP [competitive allele specific PCR (polymerase chain reaction)] technology and application of set primer")
Owner:INST OF CROP SCI CHINESE ACAD OF AGRI SCI
Reaction buffer composition for nucleic acid replication with packed DNA polymerases
The invention relates to compositions, methods, and kits for nucleic acid replication, including polymerase chain reaction (PCR) and mutagenesis reactions. A buffer composition is provided which allows higher concentrations of DNA polymerase to be used, resulting in greater yield of amplified product and faster reaction kinetics.
Owner:AGILENT TECH INC
Automatic threshold setting for quantitative polymerase chain reaction
InactiveUS20030044826A1Microbiological testing/measurementRecognisation of pattern in signalsFluorescenceBiology
Disclosed are systems and methods for identifying and quantitating the presence of one or more DNA species in a sample population through PCR amplification. DNA species quantitation includes a determination of a threshold fluorescence value used in the assessment of the PCR amplification reaction. Various embodiments of the present invention incorporate an enhancement function useful in selecting appropriate threshold fluorescence values and facilitate the determination of DNA concentrations by quantitative PCR based methodologies.
Owner:APPL BIOSYSTEMS INC
High-sensitivity real-time polymerase chain reaction for detection of nucleic acids
InactiveUS7252937B2Easy extractionImprove stabilitySugar derivativesMicrobiological testing/measurementClinical settingsHybridization probe
The invention features methods that are capable of detecting single target molecules in a sample input volume of, e.g., 5 μl, and of quantifying organismal, e.g., chiamydial, DNA. Desirably, these methods employ a single tube format coupled with fluorescent detection of amplicons. This approach facilitates the application of quantitative PCR (qPCR) to microbiological diagnosis in clinical settings. The invention also features primers and probes for the detection of Chlamydia. The use of specific hybridization probes with qPCR amplification provides the ability for identification of individual species or strains of microorganisms.
Owner:AUBURN UNIV
Polymerase chain reaction (PCR) method for diagnosing human papillomavirus (HPV) and reagent kit thereof
InactiveCN101017141AEfficient, systematic, economical and simpleShorten the timeMicrobiological testing/measurementChemiluminescene/bioluminescenceHuman bodyHuman papillomavirus
This invention relates to one polymer enzyme linkage reaction fluorescence test method to dialogue dangerous human body nipple shape virus and to isolate DNA sample HPV gene type to test one set of dangerous HPV infection from patient by the method, wherein, it belongs to life science and biological technique. This invention agent case comprises one fluorescence meter PCR technique as base of multi-layer polymer enzyme reaction composed of multiple HPV positive lead object, reaction lead object and fluorescence detector.
Owner:GENETEL PHARMA SHENZHEN
Multiplex quantitative PCR (polymerase chain reaction) detection kit for vibrio parahaemolyticus and detection method
InactiveCN102605055AMicrobiological testing/measurementFluorescence/phosphorescenceFood poisoningSaxitoxin
The invention provides a multiplex quantitative PCR (polymerase chain reaction detection kit for vibrio parahaemolyticus toxin gene and a detection method. The kit mainly comprises specific primers, probes and PCR reaction reagent, wherein the specific primers and the probes consist of specific primers and probes of vibrio parahaemolyticus thermostable direct hemolysin gene (tdh), thermolabile hemomysin gene (tlh), toxin expression regulating protein gene (toxR) and thermostable related hemolysin gene (trh). The invention provides the quick, sensitive and specific multiplex fluorescent quantitative PCR detection kit and the detection method aiming at the vibrio parahaemolyticus toxin gene, and provides basis for controlling food poisoning caused by the vibrio parahaemolyticus in time and early diagnosis of the food poisoning caused by the vibrio parahaemolyticus.
Owner:ZHEJIANG CENT FOR DISEASE CONTROL & PREVENTION
Method for detection of promoter methylation status
InactiveUS20050227265A1Precise “ molecular signature ”Accurately methylation statusMicrobiological testing/measurementFermentationCapillary electrophoresisElectrophoresis
The present invention relates to the detection of promoter methylation status using a combination of either modification of methylated DNA or restriction endonuclease digestion, multiplex polymerase chain reaction, ligase detection reaction, and a universal array or capillary electrophoresis detection.
Owner:CORNELL RES FOUNDATION INC
Nucleic acid releasing agent, nucleic acid PCR (polymerase chain reaction) amplification method and PCR amplification kit
ActiveCN109402240AGuaranteed amplification efficiencyAvoid degradationMicrobiological testing/measurementPeptidasesPotassiumPolyethylene glycol
Owner:SANSURE BIOTECH INC
Molecular identification method of atlantic salmon
ActiveCN103122386ALower requirementEasy to operateMicrobiological testing/measurementMolecular identificationEnzyme digestion
The invention discloses a molecular identification method of an atlantic salmon. The molecular identification method comprises the following steps of 1, extraction of fish DNA (deoxyribonucleic acid); 2, PCR (polymerase chain reaction) augmentation; 3, enzyme digestion and electrophoresis detection; and 4, identification. The atlantic salmon can be accurately determined in four steps. The molecular identification method of the atlantic salmon has the characteristics of being simple to operate, rapid and accurate, low in price, low in requirement on an instrument and equipment, and the like.
Owner:YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
Polymerase chain reaction-sequence based typing (PCR-SBT) method for ABO blood type genotyping and reagent
ActiveCN101921834ASolve the recombination phenomenonThe result is accurateMicrobiological testing/measurementSodium acetateHuman DNA sequencing
The invention provides a polymerase chain reaction-sequence based typing (PCR-SBT) method for ABO blood type genotyping. The method comprises the following steps of: preparing human genome DNA; amplifying segments of ABO gene exon 1, exons 2-4 and exons 5-7; performing double enzyme digestion purification on the obtained amplified products; performing a sequencing PCR reaction on the purified products; purifying the sequenced products by a sodium acetate-ethanol precipitation method and performing capillary electrophoresis sequencing; and analyzing the obtained sequences by using software to determine the genotype. The method has the advantages of solving the problems of identification of an ABO subtype, judgment of difficult blood types, discovery of a new mutational site, gene recombination among genes, genetic polymorphism detection and the like, exerting the characteristics of high flux and result accuracy of ABO genotyping operation by PCR-SBT, achieving great importance for the relative application in the fields of clinical transfusion medicinal research, genetics and the like and having important practical significance for medicinal research units, pharmic research and reagent development units.
Owner:浙江省血液中心
Digital amplification
The identification of pre-defined mutations expected to be present in a minor fraction of a cell population is important for a variety of basic research and clinical applications. The exponential, analog nature of the polymerase chain reaction is transformed into a linear, digital signal suitable for this purpose. Single molecules can be isolated by dilution and individually amplified; each product is then separately analyzed for the presence of pre-defined mutations. The process provides a reliable and quantitative measure of the proportion of variant sequences within a DNA sample.
Owner:THE JOHN HOPKINS UNIV SCHOOL OF MEDICINE
Detection of Fusarium species infecting corn using the polymerase chain reaction
InactiveUS6846631B2Detailed informationSugar derivativesMicrobiological testing/measurementFungal isolateMultiplex polymerase chain reaction
The present invention relates to the use of primers in polymerase chain reaction assays for the detection of a Fusarium proliferatum, F. verticillioides and F. subglutinans. Specific primers are identified as being useful for the identification of fungal isolates using PCR based techniques.
Owner:SYNGENTA PARTICIPATIONS AG
Amplicon Rescue Multiplex Polymerase Chain Reaction for Amplification of Multiple Targets
ActiveUS20090253183A1Enabling detectionSugar derivativesMicrobiological testing/measurementSpecific detectionPolynucleotide
Disclosed is a method for amplifying and detecting polynucleotides which can provide sensitive, specific detection of multiple targets from a clinical specimen within a relatively short time.
Owner:CB BIOTECHNOLOGIES INC +1
Method for single-tube multiplex fluorescent polymerase chain reaction (PCR) detection of human coronavirus OC43, 229E, NL63, HKU1 and SARS, and primers, probes and kit adopted by the method
InactiveCN102732638AStrong specificityHigh sensitivityMicrobiological testing/measurementDNA/RNA fragmentationHuman coronavirusFluorescein
The invention provides a method for single-tube multiplex fluorescent polymerase chain reaction (PCR) detection of human coronavirus OC43, 229E, NL63, HKU1 and SARS. The method adopts primers having sequences of SEQ ID NO: 1-10, and probes having sequences of SEQ ID NO: 11-15. The invention also provides a kit for single-tube multiplex fluorescent PCR detection of human coronavirus OC43, 229E, NL63, HKU1 and SARS. The kit contains the primers and the probes. The method provided by the invention adopts the primers which are specific primers of OC43, 229E, NL63, HKU1 and SARS, and the probes which are Taqman probes, utilizes TAMRA / CY5 / FAM / ROX / JOX multiple fluorescein labeling, realizes single-tube multiplex detection of human coronavirus OC43, 229E, NL63, HKU1 and SARS, and has the advantages of strong specificity, high sensitivity, fast detection speed, simple and convenient operation and low cost. The primers and the probes can be used as detection reagents for a scientific research and clinical application.
Owner:SUN YAT SEN UNIV
Systems and methods for performing amplicon rescue multiplex polymerase chain reaction (PCR)
ActiveUS8911949B2Heating or cooling apparatusMicrobiological testing/measurementEngineeringData transmission
Embodiments of the present disclosure generally pertain to systems and methods for performing amplicon rescue multiplex polymerase chain reaction (arm-PCR). In one embodiment, the system comprises a processor and a reader coupled to a control element. The control element is configured to control the operation of the processor and the reader based on a variety of settings. The processor is configured to receive a self-contained cassette for performing PCR amplification of DNA and / or RNA obtained from an organic specimen. The processor engages with the cassette and manipulates reagents within the cassette in order to amplify and detect the DNA from the specimen. The processor also causes the cassette to deposit the DNA on a microarray within the cassette. The reader is configured to receive the cassette after it has been processed by the processor and to capture an image of the microarray for transmission to the control element as test data. The control element is further configured to analyze the test data received from the reader and to produce an output indicative of a comparison of the test data to predefined data.
Owner:ICUBATE
Microsatellite colorectal cancer instability amplification system and detection kit thereof
InactiveCN103555843AAvoid cumbersomeReduce competitionMicrobiological testing/measurementFluorescenceInstability
The invention relates to a microsatellite colorectal cancer instability amplification system and a detection kit thereof. The amplification system disclosed by the invention comprises a general-specific chimeric primer pair and a mixture of general primers, wherein the general-specific chimeric primer pair respectively aims at microsatellite sites BAT25, BAT26, D2S123, D5S346 and D17S250; the general primer is FluD5-Up; respective positive primer 5' ends in the general-specific chimeric primer pair are provided with a sequence of the FluD5-Up; the FluD5-Up is a fluorescent-labeled primer. The amplification system and the detection kit thereof which are disclosed by the invention overcome the shortcoming that different primers require for multiple fluorescent labels; five microsatellite gene sites are detected in the same PCR (polymerase chain reaction) system, so that a result is intuitive and reliable; the time can be saved, and the efficiency is high; MSI (medium-scale integration) gene sites can be effectively detected and analyzed.
Owner:上海赛安生物医药科技股份有限公司
Method and PCR (polymerase chain reaction) reagent kit for identifying DNA (deoxyribonucleic acid) barcodes of animal medicinal materials
InactiveCN105112525AGuarantee the success rate of amplificationEfficient amplificationMicrobiological testing/measurementBetainePolyethylene glycol
The invention provides a method and a PCR (polymerase chain reaction) reagent kit for identifying DNA (deoxyribonucleic acid) barcodes of animal medicinal materials. The PCR reagent kit comprises PCR enhancers. The PCR enhancers comprise bovine serum albumin, dithiothreitol, betaine and nonidet p40. The method and the PCR reagent kit for identifying the DNA barcodes of the animal medicinal materials have the advantages that low-abundance traditional Chinese medicine samples with difficulty in amplification, particularly, samples with difficulty in identifying DNA barcodes, such as samples of buffalo horns, antelope horns, tortoise shells, carapax trionycis, pangolins and snake slough, can be effectively amplified, accordingly, the amplification success rate which is close to 100%, of DNA barcode identification techniques can be guaranteed, and DNA barcode techniques can be widely applied.
Owner:INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI
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