DNA index library building method based on high throughput sequencing

A construction method and labeling technology, which is applied in the field of nucleic acid sequencing and high-throughput sequencing, can solve the problems of inability to mix and sequence a large number of samples, low PCR amplification efficiency, and small number of labels, so as to reduce sequencing costs and increase sequencing throughput. Quantity, the effect of improving efficiency
CN102409048AActive Publication Date: 2012-04-11BGI TECH SOLUTIONS
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
BGI TECH SOLUTIONS
Publication Date
2012-04-11

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Abstract

The invention provides unique index sequences with a length of 7bp. The index sequences can be respectively imported into a DNA(deoxyribonucleic acid) index library through adapter link and PCR (polymerase chain reaction). The invention provides a method for building the DNA index library by using the index sequences based on a solexa sequencing platform of the current illumina company, and the method is applied to solexa DNA sequencing and has the effects on improving the preparation efficiency of the DNA index library, increasing the sequencing throughput of the DNA samples and lowering thesolexa sequencing cost of a single sample.
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Description

technical field

[0001] The invention relates to the technical field of nucleic acid sequencing, in particular to the technical field of high-throughput sequencing. In addition, the present invention also relates to labeling technology and a method for constructing a label library in the same reaction system for multiple samples. The method of the present invention is particularly suitable for the second generation sequencing technology, especially the solexa sequencing technology. Background technique

[0002] The Solexa DNA sequencing platform provided by Illumina can simultaneously add four kinds of fluorescently labeled nucleotides in one reaction, using Sequencing By Synthesis (SBS), which requires less sample volume, high throughput, high Accuracy, has the characteristics of simple and easy-to-operate automation platform and powerful functions [1-4]. Library construction first needs to carry out end repair on the target fragment, connect A base at the 3' end of the ta...

Claims

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