76 results about "Dna labeling" patented technology

The invention provides a method for carrying out high-throughput sequencing on a TCR (T cell receptor) or a BCR (B cell receptor). The method is characterized by designing upstream primers according to gene features of a V region of the TCR or the BCR and designing downstream primers according to gene features of a C region or a J region of the TCR or the BCR and obtaining sequences of the of the TCR or the BCR in combination with the multiplex PCR (polymerase chain reaction) technology and high-throughput sequencing, thus analyzing the rearrangement information of the TCR or the BCR. Compared with 25-30 cycles of existing multiplex PCR, two cycles of the multiplex PCR technology provided by the invention can conduce to greatly reducing the sequencing errors caused by primer amplification preference. Besides, the invention also provides a method for correcting multiplex PCR (polymerase chain reaction) primer deviation by utilizing DNA (deoxyribonucleic acid) tag sequences, thus further reducing the sequencing errors caused by primer amplification preference and intrinsic sequencing errors of high-throughput sequencing.

The invention provides unique index sequences with a length of 7bp. The index sequences can be respectively imported into a DNA(deoxyribonucleic acid) index library through adapter link and PCR (polymerase chain reaction). The invention provides a method for building the DNA index library by using the index sequences based on a solexa sequencing platform of the current illumina company, and the method is applied to solexa DNA sequencing and has the effects on improving the preparation efficiency of the DNA index library, increasing the sequencing throughput of the DNA samples and lowering thesolexa sequencing cost of a single sample.

The invention discloses DNA labels, PCR primers and application thereof. A group of the DNA labels are composed of nucleotides as shown in SEQ ID No. 79 to 129. The group of the DNA labels can be used for establishment of a nucleic acid sequencing library to realize accurate differentiation of the nucleic acid sequencing library. The sources of DNA samples can be accurately characterized by connecting the DNA labels with DNA or equivalents thereof.

DNA taggants in which the nucleotide sequences are defined according to combinatorial mathematical principles. Methods of defining nucleotide sequences of the combinatorial DNA taggants, and using such taggants for authentication and tracking and tracing an object or process are also disclosed.

The invention belongs to molecular biology DNA labeling technique and application field, concretely relates to a porphyra yezoensis microsatellite marker screening method, and a method for analyzing germplasm genetic diversity by the makers, thereby lay foundations for genetic structure analysis of porphyra yezoensis, germplasm resource protection and molecular marker auxiliary breeding.

The invention is applicable to the field of biological engineering, and provides an environmental microorganism detection method and a system thereof. The method comprises the following steps: sequencing the DNA extracted from environment samples by the high throughput sequencing technology to obtain a DNA tag sequence; eliminating carrier pollution existing in DNA tag sequence; comparing the DNAtag sequence with the known sequence in the known database, and determining the category to which the DNA tag sequence belongs according to the comparison result. The embodiment of the invention can detect which microorganism species or which class of microorganism species possibly exists in environment samples.

The invention designs 161 unique index sequences with a length of 8bp, and the indexes are embedded into DNA PCR (deoxyribonucleic acid polymerase chain reaction) primers to form DNA PCR index primers, thus being capable of importing the index sequences through PCR. The invention successfully establishes a method for building a DNA index library, and the method is applied to solexa DNA sequencing.

Food distributed to consumers through a distribution chain may be traced by tagging the food with DNA tags that identify the origin of the food, such as the grower, packer and other points of distribution, and their attributes. This makes it much quicker and easier to trace the food in case of food contamination or adulteration. Preferably these attributes indicate the field, location, crew and machine used to grow and process the food, and the dates of the various steps of food harvesting, processing and distribution. Natural or synthetic DNA pieces may be used to tag items, including food items. Multidigit binary or other types of bar codes may be represented by multiple types of DNA. Each digit of the bar code may be represented by one, two or more unique DNA pieces.

The invention provides a set of DNA index and application thereof to construction and sequencing of mate-paired indexed library, and the DNA index has a sequence selected from SEQ ID NO:1-24. The invention also provides a method for construction and sequencing of the mate-paired index library. The method employs two independent sequencing reactions to realize mixed sequencing on a plurality of mate-paired indexed libraries in a singe sequencing chip, so as to accelerate high flux sequencing, and reduce time, reagent cost and output cost of unit data.

The invention provides a method for enriching viruses from faeces rapidly and efficiently, amplifying signals through gold nanoparticles crosslinked through specific DNA labels and then rapidly detecting early-state PEDV infection with a PCR. According to the method, an PRF1a gene located in a PEDV whole genome is adopted as a target sequence, specific nucleic acid probes are designed, second-segment probes which are high in specificity, great in virus enriching capability and easy to amplify and detect are screened out of the specific nucleic acid probes and optimized, and by means of functionalized magnetic particles and nanogold particles of the second-segment probes, a PCR detection technology based on nanoparticle PEDV specificity is established; meanwhile, by means of the technical method, the technical breakthrough of conducting a direct hybridization reaction between faeces sample lysate and the functionalized magnetic particles is achieved, the step of purifying a sample nucleic acid is completely omitted, specificity and sensitiveness are improved, meanwhile, the detection step is simplified, and detection time is shortened. By means of the method, time and labor are saved, cost is low, and in addition, the animal virus infection level can be prompted accurately.

A method is provided for assessing allelic losses and hypermethylation of genes in CpG tumor promotor region on specific chromosomal regions in cancer patients, including melanoma, neuroblastoma breast, colorectal, and prostate cancer patients. The method relies on the evidence that free DNA and hypermethylation of genes in CpG tumor promotor region may be identified in the bone marrow, serum, plasma, and tumor tissue samples of cancer patients. Methods of melanoma, neuroblastoma, colorectal cancer, breast cancer and prostate cancer detection, staging, and prognosis are also provided.

The invention is suitable for the field of bioengineering and provides a method and a system for detecting environmental microorganisms. The method comprises the following steps: DNA extracted from an environmental sample is sequenced by the high-flux sequencing technology, and DNA label sequence is obtained; vector pollution in the DNA label sequence is removed; and the DNA label sequence is compared with the known sequence in a known database, and the class of the DNA label sequence is determined according to the comparison result. The embodiment of the method can detect possible microbial species and possible varieties of microbial species in the environmental sample.

The invention provides a noninvasive biopsy virus detection method based on high throughput gene sequencing. The method can carry out DNA sequencing and RNA sequence in parallel and thus precisely determines the biological composition and structure of viruses in patients. The method can maximally avoid the interference of nucleic acid of host cells, effectively reduce the cost of sequencing, and is capable of detecting multiple unknown crossed viruses at the same time. 80 DNA tagged connectors are designed, related reagents are assembled to form a library so as to construct a kit, and if the throughput allows, the kit can perform sequencing on 80 samples in parallel. The experiment design is strict, the automation degree is high, the virus identification can be finished in batches within 24 hours, the requirements of scientific research and clinical detection can be met in different levels, and references are provided for clinical diagnosis and prescription of diseases related with virus infection.

The invention relates to a DNA label and PCR primer pairs with the 5'-end connected with the DNA label, and provides a sequencing library construction method adopting the DNA label and the PCR primer pairs, and a kit composed of the DNA label and the PCR primer pairs. The DNA label and amplimers form a detection product with optimized and balanced specificity, sensitivity and repeatability, the detection product can be used to detect 1-20 different sources of a sample once, and can accurately distinguish the base sequence of various sample sources. The coincidence rate of high flux sequencing and a sequencing method reaches up to 100%.

The invention belongs to the field of molecular biology DNA labeling technology and application, and in particular relates to Apostichopus japonicas express sequence tag EST micro-satellite label screening development and application. The invention provides Apostichopus japonicas AjE101 micro-satellite specific DNA primers, a polymerase chain reaction (PCR) reaction system by utilizing the primers, and a detection method for an Apostichopus japonicas AjE101 micro-satellite DNA label. The detection method comprises the following steps of: extracting genome of Litopenaeus vannamei Boone; designing the specific primers at two ends of an Apostichopus japonicas AjE101 micro-satellite DNA core sequence; and performing PCR amplification on genome DNA of different groups of the Litopenaeus vannamei Boone or individuals in the group by using the primers, analyzing products, and determining the genome of each individual so as to obtain an Apostichopus japonicas polymorphism genetic variation map. The invention is mainly applied to Apostichopus japonicas germ plasm resource and genetic diversity analysis, cular population genetics, construction of genetic maps and research of functional genes.

The invention relates to a method for using rice auxin to induce protein genes to be cloned, which is characterized in that the implementation steps comprise: (1) the preparation of a rice transformation receptor; (2) the genetic transformation of the rice; (3) the screening of kanamycin-resistant callus tissue and the regeneration of plants; (4) the screening of the mutant of a T-DNA inserted progenies; (5) Tail-PCR; (6) the comparison and analysis of sequences on the Internet. In the invention, a rice mutant with a short plant height is obtained when carrying out rice functional genome research by using T-DNA label method; the lateral neighboring sequences of the mutant are researched by using TAIL-PCR technology; meanwhile, the position where the mutant T-DNA inserts the rice genome is arranged on the No. 4 chromosome of the rice by the comparison on the databases of NCBI and TIGR on the Internet; moreover, the rice BAC clone (OSJNBa0084K01) of the position is found out. The T-DNA is inserted between the two genes of the clone by analyzing the clone. Known functional genes with a very high homology with BAC cloning code amino acid sequence are forecasted by the sequence comparison on the Internet.

The invention provides an identification method for a sequencing sample and an application thereof. The method comprises the following steps: (1) constructing an injective relationship between a sequencing sample library and a DNA tag library; (2) according to the injective relationship described in the step (1), using a DNA tag in the DNA tag library to identify a sequencing sample. The method determines a DNA tag combination uniquely corresponding to the sequencing sample by constructing the injective relationship between the sequencing sample library and the DNA tag library, and different sequencing samples are corresponding to different DNA tag combinations, thereby realizing specificity identification of the sequencing samples by the DNA tags.

The invention discloses DNA tags, a PCR primer and application thereof. The DNA tags of one group are chosen from nucleotides shown as in SEQ ID NO:1-95 and can be used for building a nucleic acid sequencing library to accurately differentiate the nucleic acid sequencing library. The DNA tags and the PCR primer are utilized to build a tagged PCR primer, and by utilizing the tagged PCR primer, STR detection of at most 95 DNA samples can be realized at one step according to a method for determining preset STR gene locus genotype of various DNA samples.

A method for single-molecule optical DNA profiling using an exceptionally dense, yet sequence-specific coverage of DNA with a fluorescent probe, using a DNA methyltransferase enzyme to direct the DNA labeling, followed by molecular combing of the DNA onto a polymer-coated surface and subsequent sub-diffraction limit localization of the fluorophores. The result is a ‘DNA fluorocode’; a simple description of the DNA sequence, with a maximum achievable resolution of less than 20 bases, which can be read and analyzed like a barcode. The method generates a fluorocode for genomic DNA from the lambda bacteriophage using a DNA methyltransferase to direct fluorescent labels to four-base sequences reading 5′-GCGC-3′. A consensus fluorocode is constructed that allows the study of the DNA sequence at the level of an individual labeling site and is generated from a handful of molecules and entirely independently of any reference sequence.

The invention discloses a DNA label, a PCR primer and application thereof. The DNA label is chosen from nucleotides shown as SEQ ID NO:1-95 and can be used for building a nucleic acid sequencing library to accurately differentiate the nucleic acid sequencing library. The DNA label and the PCR primer are utilized to structure a label PCR primer, and deafness gene mutation detection of 95 DNA samples can be realized at most in one time by utilizing the label PCR primer according to a method for determining whether deafness genes of various DNA samples are mutational or not.

A novel method of organism identification that can replace the conventional organism identification method by DNA requiring a large amount of labor. There has been developed a method of organism identification, namely, a method of identifying whether or not being a specified organism or a descendant thereof, characterized in that whether or not an identification object organism is a specified organism or a descendant thereof is judged by introducing in advance a DNA tag in cells of the specified organism or organism living parasitically or symbiotically therewith and finding whether the DNA tag is detected or not from the DNA extracted from the identification object organism or organism living parasitically or symbiotically therewith.