Method for rapidly and accurately breeding sweet and glutinous double recessive corn selfing line

A technology of sweet and waxy double and inbred lines, which is applied in the field of crop cultivation, can solve the problems of time-consuming breeding process and increase the time for creating new germplasm, and achieve the effect of improving the efficiency of breeding

Pending Publication Date: 2018-11-16
HEBEI AGRICULTURAL UNIV.
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Problems solved by technology

[0003] Sweet and waxy corn breeding relies on double recessive (such as wxwxsh2sh2) inbred lines as parents, but the existing conventional breeding of sweet and waxy double recessive inbred lines only relies on phenotypic identification, so the breeding process is time-consuming and new species have been added quality creation time

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  • Method for rapidly and accurately breeding sweet and glutinous double recessive corn selfing line
  • Method for rapidly and accurately breeding sweet and glutinous double recessive corn selfing line
  • Method for rapidly and accurately breeding sweet and glutinous double recessive corn selfing line

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Embodiment Construction

[0036] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without creative work, any modifications, equivalent replacements, improvements, etc., shall be included in the protection scope of the present invention Inside.

[0037] Such as Figure 1 to Figure 2 As shown, the method for quickly and accurately breeding sweet and waxy double recessive corn inbred lines of the present invention, the steps are:

[0038] S1, extracting the DNA of sweet corn;

[0039] S2. Use the specific primers T1 and T2 designed according to the mutation site characteristics of the sh2 sequence to detect the sweet corn sh2 gene...

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Abstract

The invention discloses a method for rapidly and accurately breeding a sweet and glutinous double recessive corn selfing line. The method comprises following steps: taking a sh2sh2 type super-sweet corn as a donor parent and a wxwx corn that is in accord with the breeding target as a receptor parent to establish BC1F1; dyeing BC1F1 seeds by an iodine staining method, screening wxwx germplasm namely seeds with a light color; subjecting wxwx germplasm to DNA mark detection to screen out germplasm with SH2sh2, for each backcrossing generation, carrying out iodine staining and DNA labeling to obtain target seeds, backcrossing to BC3F1, and carrying out selfing to obtain BC3F2, wherein the wrinkled seeds are the sweet and glutinous double recessive corn selfing line. The method has the advantages that specific primers (T1, T2) are used to carry out detection so as to accurately and efficiently screen out individuals containing a recessive sh2 gene, and through the directional selection of backcrossing generation, the breeding efficiency is obviously improved.

Description

technical field [0001] The invention relates to the technical field of crop cultivation, in particular to a method for quickly and accurately breeding sweet and waxy double recessive corn inbred lines. Background technique [0002] Fresh corn is divided into sweet and waxy corn. Because of its unique flavor and nutritional content, it is favored by consumers. With the change of dietary structure, people's demand for fresh corn is increasing year by year. In particular, the acceleration of rural urbanization in my country and the optimization of the agricultural industry structure have led to an increase in the demand for fresh corn. Therefore, the creation of new germplasm of fresh corn has become an important research content and direction for fresh corn breeding and fresh corn breeders. It lays an important foundation for the selection and breeding of sweet waxy corn varieties. [0003] Sweet and waxy corn breeding relies on double recessive (such as wxwxsh2sh2) inbred l...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02C12Q1/6895
CPCA01H1/02C12Q1/6895
Inventor 段会军张建恒代亮么大轩
Owner HEBEI AGRICULTURAL UNIV.
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