138 results about "Stain method" patented technology

A deep learning-based virtual HER2 IHC staining method uses a conditional generative adversarial network that is trained to rapidly transform autofluorescence microscopic images of unlabeled / label-free breast tissue sections into bright-field equivalent microscopic images, matching the standard HER2 IHC staining that is chemically performed on the same tissue sections. The efficacy of this staining framework was demonstrated by quantitative analysis of blindly graded HER2 scores of virtually stained and immunohistochemically stained HER2 whole slide images (WSIs). A second quantitative blinded study revealed that the virtually stained HER2 images exhibit a comparable staining quality in the level of nuclear detail, membrane clearness, and absence of staining artifacts with respect to their immunohistochemically stained counterparts. This virtual staining framework bypasses the costly, laborious, and time-consuming IHC staining procedures in laboratory, and can be extended to other types of biomarkers to accelerate the IHC tissue staining and biomedical workflow.

The invention provides a papillary thyroid carcinoma lymph node metastasis prediction method, system and equipment. The method comprises the following steps: acquiring a staining collagen image of a first papillary thyroid carcinoma section stained by a hematoxylin eosin staining method; obtaining a multi-photon collagen image of the second papillary thyroid carcinoma slice through a multi-photon imaging method; setting an observation area on the multi-photon collagen image according to the dyed collagen image; extracting collagen characteristics in the observation area; constructing a collagen feature scoring model according to an LASSO algorithm and the collagen features; obtaining a collagen feature score according to the collagen feature scoring model; acquiring clinical risk factors; the papillary thyroid carcinoma metastasis risk information is output according to the clinical risk factors and the collagen feature scores, the collagen features in the papillary thyroid carcinoma microenvironment can be accurately and quantitatively extracted by combining the multi-photon imaging technology and collagen feature analysis, accurate prediction of the papillary thyroid carcinoma metastasis risk is achieved, and the accuracy of the papillary thyroid carcinoma metastasis risk is improved. And the prediction accuracy can be further improved in combination with clinical risk factors.

The utility model relates to the technical field of medical instruments, in particular to a blood pushing piece dyeing device which comprises a rotary conveying mechanism, four blocking and separating mechanisms, a reagent filling and discharging mechanism, a plurality of single piece dyeing boxes and a bottom plate. The four blocking and separating mechanisms are distributed in the circumferential direction of the rotary conveying mechanism at a certain angle, the reagent filling and discharging mechanism is fixedly connected with the rotary conveying mechanism and located above the rotary conveying mechanism, and the multiple single-piece dyeing boxes are placed in a rotary disc of the rotary conveying mechanism. The motor drives the rotary conveying mechanism to drive the single-piece dyeing box to rotate, and the screw rod motor drives the reagent filling and discharging mechanism to fill and discharge reagents into the single-piece dyeing box. And after dyeing is finished, the bleeding smear is taken, and the single-piece dyeing box continues to rotate, so that recycling is realized. The whole staining process is automatically carried out, and the technical problem that a traditional blood smear staining method is poor in effect is solved.

A deep learning-based digital staining method and system are disclosed that enables the creation of digitally / virtually-stained microscopic images from label or stain-free samples based on autofluorescence images acquired using a fluorescent microscope. The system and method have particular applicability for the creation of digitally / virtually-stained whole slide images (WSIs) of unlabeled / unstained tissue samples that are analyzes by a histopathologist. The methods bypass the standard histochemical staining process, saving time and cost. This method is based on deep learning, and uses, in one embodiment, a convolutional neural network trained using a generative adversarial network model to transform fluorescence images of an unlabeled sample into an image that is equivalent to the brightfield image of the chemically stained-version of the same sample. This label-free digital staining method eliminates cumbersome and costly histochemical staining procedures and significantly simplifies tissue preparation in pathology and histology fields.

The invention relates to the field of ecological environment, in particular to a method for characterizing the influence of PPCPs on the toxicity of an activated sludge system by using a viable and dead bacteria staining method, which comprises the following steps of: improving an SYTO 9 dye and PI dye cell staining method, namely quantitatively detecting the fluorescence intensity corresponding to dead bacteria and viable bacteria by using a three-dimensional fluorescence spectrophotometer; a quantitative detection technology of flora activity is established, and a set of method capable of rapidly and accurately quantifying the ratio of dead bacteria to viable bacteria in a sample is constructed; according to the invention, degradation conditions of PPCPs and organic matters in water with different concentrations and different PPCPs under the same operation condition and a change rule of water toxicity are explored, the influence of PPCPs on the toxicity of an activated sludge system is represented by using a viable bacteria and dead bacteria staining method, and the influence degree of PPCPs on a sewage treatment system can be rapidly analyzed through relative fluorescence intensity. Therefore, a rapid and effective detection method is provided for monitoring the influence of the PPCPs in the inlet water of the sewage plant on the treatment process of the water plant, and the method has a wide application prospect.

This invention provides a PAM staining method that enables staining suitable for magnified observation of various samples using a range of microscopes, from optical microscopes to electron microscopes. [Solution] A PAM staining method comprising the steps of: silvering a biological sample; coloring the biological sample; and fixing the color of the biological sample, wherein the biological sample is a biologically derived sample that has been oxidized; the silvering step involves immersing the biological sample in a methenamine silver aqueous solution at room temperature, raising the methenamine silver aqueous solution from room temperature to 62°C or higher and 68°C or lower, and carrying out the silvering reaction for 30 to 40 minutes from the start of the reaction at room temperature; the coloring step involves immersing the silvered biological sample in a 0.2% gold chloride aqueous solution at room temperature for 8 to 12 hours; and the fixing step involves immersing the sample in a hard film fixing solution containing 11% to 13% sodium thiosulfate and aluminum potassium sulfate dodecahydrate for 3 minutes or more at room temperature.

The invention relates to the technical field of chemiluminescence immunoassay, and discloses a horse radish peroxidase-labeled secondary antibody compound and a preparation method and application thereof, the horse radish peroxidase-labeled secondary antibody compound comprises a chitosan compound skeleton, and horse radish peroxidase and a secondary antibody carried on the chitosan compound skeleton; the chitosan compound skeleton is a macromolecular skeleton compounded by a chitosan spherical cross-linking body and dopamine. Through optimization and improvement of a polymer skeleton, the problems of large steric hindrance, low sensitivity and non-specific staining of a traditional polymer can be solved, and further improvement of the sensitivity of a DAB staining method for immunoassay detection is facilitated; the adopted chitosan compound skeleton has abundant amino groups, can bear more labeled enzymes and secondary antibodies, reduces steric hindrance, increases the penetrating power to a cell nuclear membrane, and improves the detection sensitivity.

The invention belongs to the field of biochemical detection equipment, and discloses a flaking and dyeing all-in-one machine and a flaking and dyeing method.The flaking and dyeing all-in-one machine comprises a machine base, and a workbench, a moving mechanism and a manipulator assembly are arranged on the machine base; the working table is provided with a working area, and the working area is at least provided with a pretreatment working position and a flaking and dyeing working position; the manipulator assembly is movably arranged on the moving mechanism, and the manipulator assembly is located above the working area; the manipulator assembly comprises manipulators, the manipulators at least comprise a first manipulator and a second manipulator, the first manipulator at least has a first moving position and a second moving position, and the second manipulator at least has a third moving position and a fourth moving position; and the first moving position, the second moving position, the third moving position and the fourth moving position are at least matched with the pre-treatment working position and the flaking and dyeing treatment working position. According to the slide preparation and dyeing all-in-one machine, slide preparation and dyeing of liquid-based sample cells can be integrated into all-in-one equipment, and all steps can be independently and circularly carried out.

The invention relates to a cell cleaning solution, a reagent combination for serosal cavity liquid-based cell treatment, a serosal cavity liquid-based cell staining kit and a staining method, and belongs to the technical field of biological detection. In order to consider the morphology and the ICC dyeing effect, the invention provides a formula of the cell cleaning fluid based on a large number of experimental studies in the early stage, and subsequent experiments prove that when the cell cleaning fluid is used for treating a bloody specimen, the effects of rapid hemolysis, mucus and protein fluid removal and the like can be realized, and meanwhile, other cells are effectively fixed; the morphological integrity and good antigenicity of other cells are ensured. And a thin and uniform cell layer is prepared through natural sedimentation, is suitable for cytological detection, has a clear background, and can completely show the morphology and structure of cells. The cell cleaning fluid is a medium for cleaning and dissolving red blood cells, can generate a destructive effect on the red blood cells, and is an effective reagent capable of ensuring the morphological integrity and good antigenicity of other cells.

The application discloses a kind of for carcinosarcoma evaluation section and its preparation method and application, belong to pathological technical field.The preparation method includes the following steps: reticular fiber staining: silver staining method is carried out to the reticular fiber in section tissue and is dyed;Immunohistochemical staining: AE1 / AE3 protein in section tissue is marked and dyed by immunohistochemical staining method.The section obtained by the preparation method can present two kinds of staining on a tissue section, improve the expression effect of cancer, sarcoma, carcinosarcoma, reduce the identification difficulty, and be beneficial to tumor accurate typing.

The invention discloses a method for predicting the curative effect of triple negative breast cancer immunotherapy by spatially quantizing CD39 + CD103 + CD8 + T cells, and relates to the technical field of biology.The method comprises the following specific steps that S1, an FFPE tumor tissue sample of a TNBC subject before immunotherapy is obtained, and a continuous section with the thickness of 3-5 microns is prepared and attached to a positive charge glass slide; s2, staining the section by using a combination containing CD8 / CD103 / CD39 / Pan-CK antibodies through a TSA sequential staining method, and then re-staining cell nucleuses by using DAPI; according to the method, spatial quantitative analysis is performed on a specific CD39 + CD103 + CD8 + T cell subset, a percentage index of target cells in CD8 + T cells and an average distance index of the target cells and nearest Pan-CK positive cells in a cancer nest region are set, and the target cells are determined according to comparison between the two indexes and a preset threshold value. According to the method, whether a subject is a potential benefit crowd treated by an anti-PD-1 / PD-L1 immune checkpoint inhibitor or not is accurately judged, a standardized technical means is provided for clinically screening the potential benefit crowd for immunotherapy, the accuracy and effectiveness of triple-negative breast cancer immunotherapy are effectively improved, and invalid treatment is reduced.

The invention relates to a method for staining bacteria, comprising the steps of a) staining a bacteria sample by means of a solution of crystal violet, b) adding Lugol's solution to the stained bacteria sample, c) rinsing with distilled water, d) adding a solution of safranine-O and fuchsin in diethylene glycol monoethyl ether, and e) rinsing with distilled water, wherein the method is carried out substantially without alkanol.

The present application relates to the field of plant biotechnology, and particularly relates to a fluorescence staining method for pollen germination of Dendrobium nobile Lindl, which comprises five steps: collecting Dendrobium nobile Lindl flowers with a stigma, removing extra parts such as sepals and only keeping the stigma, trimming the two sides of the stigma into a horizontal shape, placing it into a pre-cooled fixing solution for fixation, and then dehydrating it with gradient alcohol; cutting the dehydrated stigma along the middle line of the stigma with a scalpel; placing the cut sample into a softener for treatment to soften the tissue; transferring the transparent sample into a staining solution containing fluorescent dye for staining in dark conditions; after the completion of staining, taking out the sample, fixing it on a glass slide, covering it with a cover glass, and observing the pollen germination of the Dendrobium nobile Lindl stigma under a fluorescence microscope. The method can clearly and accurately observe the pollen germination of the Dendrobium nobile Lindl stigma, and provides strong technical support for the related research of Dendrobium nobile Lindl and other plant materials with large stigmas.

This invention discloses a high-throughput automated immunofluorescence staining device and its staining method. The device includes a liquid injection motion module, a reaction chamber module, a supply and waste collection module, and a control system. The liquid injection motion module includes an X-axis beam and a pipetting and auxiliary material robotic arm, which are driven in parallel to achieve sequential operation. The reaction chamber module includes a temperature-controlled station, a low-temperature reagent storage unit, and an auxiliary material supply unit. The supply and waste collection module includes a waste removal and cleaning unit and an auxiliary material waste collection unit. This invention achieves a 96-station array through a temperature-controlled reaction station, combined with the dual-drive parallel operation of the pipetting and auxiliary material robotic arms, eliminating action waiting time and significantly improving throughput. The use of a closed semiconductor cooling component linked with an automatic flip-top mechanism ensures uniform reagent activity over a long period. A plate-mounted small vacuum pump drives a rigid pipeline with deep-hole waste suction, combined with high-flow-rate wide-area spraying, to thoroughly remove waste liquid and reduce cross-contamination. This achieves full-process automation, significantly improving staining quality and efficiency.

The invention discloses a dyeing method and kit for an osteocyte indentation-tubule system, and the dyeing method comprises the following steps: (1) preparing (0.5-1) mol / L AgNO3 as a solution I, (2) preparing 0.05%-2% (w / v) of B-type gelatin and 0.05%-5% (v / v) of formic acid as a solution II; and (3) mixing the solution I and the solution II in a volume ratio of (4-1): 1, and dyeing. The optimized dyeing step shows clear and reliable dyeing mode and strength in different species and disease models, and compared with a traditional method, the method has the advantages that more bone cell indentation-tubule systems can be dyed, and the density of the bone cell indentation-tubule systems can be increased.

Staining devices and methods for automated systems are provided herein. A dyeing method includes disposing a substrate proximate a dyed surface to establish an overlap between the dyed surface and the substrate. The staining method further includes maintaining a gap between the stained surface and the substrate to define a chromosome product according to the overlap and the gap, where the chromosome product has one or more meniscus edges. The staining method further includes disposing a portion of the staining fluid over the staining surface and distributing the staining fluid over the substrate.

The invention discloses a data staining method, system and device and a storage medium, and relates to the technical field of data security, the method comprises the following steps: extracting statistical features of training data, and obtaining predefined staining features; using a feature generator to fuse the statistical features and predefined dyeing features to obtain fused features, and training a dyeing model based on the fused features so as to embed the predefined dyeing features into dyeing data generated by the dyeing model; and detecting whether the dyeing data carries the predefined dyeing characteristics or not through a traceability model so as to determine the source of the dyeing data. By training the dyeing model, the generated dyeing data keeps the characteristics of the original data, and the invisible statistical beacons are embedded, so that the security and traceability of the data are ensured. By detecting whether the dyeing data carries the predefined dyeing features, the source of the data can be accurately identified, and the authenticity and integrity of the data are ensured.

The invention relates to the technical field of organic molecular fluorescent probes, in particular to a near-infrared fluorescent probe for detecting and marking micro-plastics as well as a preparation method and application of the near-infrared fluorescent probe HCY-PS. The near-infrared fluorescent probe HCY-PS is prepared by taking hemicyanine dye as a framework and introducing 4-dimethylamine-1-naphthaldehyde. The fluorescent probe HCY-PS is combined with the micro-plastic through electrostatic adsorption, pi-pi accumulation and hydrophobic interaction, and the micro-plastic is marked through a dyeing method. The fluorescent probe HCY-PS disclosed by the invention has a wide linear response range on the micro-plastic and high detection sensitivity, and the detection limit is 0.153 mg / L. The fluorescent probe HCY-PS provided by the invention can be applied to cell level detection and microplastic marking. The fluorescent probe HCY-PS disclosed by the invention can be applied to horizontally marking micro-plastics in an organism. The fluorescent probe HCY-PS is obtained through one-step condensation, harsh reaction conditions are not needed, and mass production is possible.

The present invention relates to the technical field of biology, and discloses a lefuchsin staining method kit, which comprises a lefuchsin staining solution and a collagenous fiber staining method staining solution, the solution is prepared from the following raw materials by mass: 2-20 g of basic fuchsin, 4-40 g of resorcin, 29-35 g of ferric trichloride, 5-20 g of hematoxylin, 1-20 g of acid fuchsin, 2.5-10 g of picric acid, 3-10 ml of hydrochloric acid, and 2000 ml of pure water. When the staining solution of the kit is used for staining, no staining solution precipitation residue exists, no oxidation film exists on the staining solution, and the elastic fibers can be dyed into the elastic fibers with dark blue to black blue, the collagenous fibers are red, the muscle fibers and red blood cells are yellow, and the cell nucleuses are black blue in an extremely short time.

The invention relates to a wine stain treatment system based on stain characteristics and dual-wavelength parameter matching, and the system comprises a data collection module which is used for collecting a wine stain region parameter, a patient skin parameter and a historical treatment parameter of a patient; presetting a dual-wavelength wine stain treatment method library; the color spot mapping module is used for extracting color spot characteristic parameters from the wine color spot area parameters and determining adaptive matching values of the color spot characteristic parameters and the dual-wavelength treatment methods so as to construct a characteristic mapping matrix; the matrix optimization and adjustment module is used for extracting a core influence factor and a weighting coefficient from the skin parameters of the patient and carrying out weighting operation and adjustment on the core influence factor and the weighting coefficient and the feature mapping matrix; the treatment method matching module is used for matching with a method library according to the feature mapping matrix; and the color spot treatment module is used for setting a dual-wavelength light source to perform color spot treatment according to the acquired dual-wavelength treatment method. Compared with the prior art, the method has the advantage that the safety and the personalized adaptability of a spot treatment scheme are improved.

The invention discloses a method for collecting and dyeing sperms of Chinese prawns, and belongs to the technical field of germplasm resource protection and utilization. The method comprises the following steps: grinding Penaeus chinensis spermatophore with outer membrane removed in a glass homogenizer, adding sterilized artificial seawater, grinding until the solution becomes milky white, and filtering to obtain sperm; and mixing the sperm with eosin-nigrosine dye liquor, and standing to obtain the dyed Chinese prawn sperm. The collecting and dyeing steps are simple and easy to operate, the whole process is short in time consumption, small in sperm damage, visual in effect and high in sperm survival rate. The staining method disclosed by the invention is not only suitable for staining fresh sperms, but also can be used for staining the thawed and resuscitated sperms after cryopreservation, the staining observation after thawing is clear, and the sperms are not easy to crystallize.

The use of AST-3424 in the preparation of a drug for treating a patient with hepatocellular carcinoma. A pathological paraffin block or pathological section of ex-vivo liver tumor tissue from the patient with hepatocellular carcinoma is subjected to immunohistochemical staining to detect the protein expression level of an AKR1C3 enzyme, with the detection result showing an H-score greater than or equal to 200; or the pathological paraffin block or pathological section of ex-vivo liver tumor tissue from the patient with hepatocellular carcinoma is subjected to immunohistochemical staining to detect the protein expression level of an AKR1C3 enzyme, with the detection result showing that the sum of percentages of moderate-intensity staining and high-intensity staining is greater than or equal to 70%. The dosing regimen for AST-3424 is as follows: in each 21-day cycle, administration is performed on day 1 and day 8; the initial dose is 6.0 mg / m2 per administration; and the patient will be permitted to receive treatment for a maximum of 34 cycles.

A method for staining reticulocytes includes staining reagent A and stabilizing reagent B; stabilizing reagent B is used M seconds after staining reagent A is mixed with the sample; M is greater than or equal to 20 seconds; staining reagent A includes staining agent A10, adjusting salt C10, and water; staining agent A10 is neomethylene blue; the concentration of neomethylene blue in staining reagent A is 0.8 × 10⁻⁶. ‑4 g / ml ~ 8.0 × 10 ‑4 g / mL; adjusting salt C10 is used to provide a low osmotic pressure environment and a pH range of 7.0–8.0; stabilizing reagent B includes staining stabilizer B10, adjusting salt D10, and water; the staining stabilizer B10 in stabilizing reagent B includes aldehydes. A unique two-step reagent formulation and method are used to achieve rapid staining of reticulocytes in a step-by-step manner, achieving successful staining in just 20 seconds, and the staining effect can be maintained for a sufficiently long time, exceeding 120 minutes.

The invention provides a method for detecting natural killer cells on a flow cytometer and a kit thereof, the method is a staining method for detecting the natural killer cells, and the method promotes separation of a CD3-(CD56 + CD16) + cell population from a CD3-(CD56 + CD16)-cell population; the method comprises the following steps: washing whole blood with a buffer solution to obtain a cell suspension, mixing a CD45 dyeing antibody, a CD3 dyeing antibody, a CD16 dyeing antibody and a CD56 dyeing antibody with the cell suspension, carrying out incubation dyeing, adding a lysis solution after dyeing, carrying out erythrocyte lysis, and finally carrying out up-flow cytometry detection. According to the method disclosed by the invention, the separation degree of the NK cells and the non-NK cells is remarkably increased, and the cell grouping of an unclear NK grouping sample in a first-washing and second-dyeing mode is remarkably improved.

The invention relates to the technical field of tissue section staining, in particular to a multicolor composite staining kit, a staining method and application of the multicolor composite staining kit. The multi-color composite staining kit provided by the invention not only can realize a better staining effect and has high discrimination on staining of different tissue structures, but also does not contain dangerous chemical components such as picric acid, ethanol, ammonia and the like, so that related risks and costs are greatly reduced in the processes of production, storage, transportation, use and discarding; in addition, by adopting the kit disclosed by the invention, the operation steps can be effectively simplified, the operation difficulty is reduced, the repeatability of a dyeing result is remarkably improved, meanwhile, the kit is more friendly to the environment and operators, the cost is lower, and the kit has a better application prospect.

The invention relates to a dyeing kit and a dyeing method for colocalization evaluation of enteritis model pathology. The staining kit comprises the following components: a hematoxylin staining solution, an alcian blue staining solution, a periodic acid solution, a Schiff reagent, a dewaxing antigen repair solution, a peroxidase inhibitor, a direct-use primary antibody CD4, a non-specific antigen confining liquid, a direct-use secondary antibody HRP-goat anti-rabbit IgG polymer, a DAB color developing agent, a phosphate buffer solution, a Tris buffer salt solution and a phosphate buffer solution detergent. And a transparent agent. According to the method, histological pathological characteristics of an enteritis model and a scoring system thereof are ingeniously fused, multiple staining which needs to be completed by three slices traditionally can be integrated to one slice by adopting a single slice, complex steps of multiple staining methods in the early-stage slice preparation process are simplified and accelerated, time consumption is reduced, it is ensured that the slices can be completed more quickly, and the efficiency is improved. Therefore, the next analysis stage is timely entered.

The present invention discloses a staining method for co-staining of three fibrous tissues, which includes the steps of successively using Victoria blue staining and Gordon-Sweets silver ammonia solution staining on the processed tissue sections; the staining method of the present invention combines Victoria blue staining and silver ammonia solution staining, and can clearly present the staining of three fibrous tissues on the same section, clearly show the relationship between the cell nucleus structure and elastic fibers, significantly improve the diagnostic accuracy of the degree of tumor invasion, reduce the demand for tissue samples, and the staining method of the present invention has the advantages of clear color contrast, rich levels, accurate positioning, simple operation, etc., and has important significance for clinical pathological diagnosis and is worthy of popularization and application.

This invention relates to the field of immunoassay technology, specifically to a multicolor fluorescent diagnostic reagent for detecting PAP. The multicolor fluorescent diagnostic reagent includes Hurst dye, wheat germ lectin, fluorescein isothiocyanate, a counterstaining agent, an organic solubilizer, and a humectant. This invention incorporates multiple fluorescent dyes, enabling rapid and effective detection of glycoprotein signals in PAP patient specimens. Simultaneously, it clearly displays cell morphology in the specimens, allowing for timely exclusion of bacteria, fungi, and abnormal cells, thus helping doctors quickly and accurately determine the type of PAP in patients. Compared to the traditional PAS staining method, the multicolor fluorescent diagnostic reagent provided by this invention uses a one-step staining method, which is simple to operate, has a short detection time, and allows for rapid observation of test results, facilitating timely diagnosis and treatment for patients.

The present application relates to a kind of hematoxylin-eosin (HE) staining method and operation steps without xylene and alcohol, belong to pathological technique, apply in pathology, histology and clinical experimental diagnosis biomedical disciplines field such as embryo. The hematoxylin-eosin (HE) staining step of conventional paraffin section is at least 12 more additional steps before and after dyeing into dyeing liquid dyeing to mounting respectively needs to use including xylene and gradient concentration alcohol. Lengthy dyeing step is not only laborious and time-consuming, also pollutes air environment and harms personnel health. The present application is a kind of conventional paraffin section whole process hematoxylin-eosin (HE) dyeing step without using xylene and alcohol method and step, make its application in management HE dyeing process for improving pathological technical room work quality and efficiency, reduce reagent cost and eliminate environmental pollution is crucial.

An analysis support method for supporting analysis of stained images of a sample includes preparing multiple stained images by applying multiple types of staining methods on one sample, the multiple stained images corresponding respectively to the multiple types of staining methods (step S11), segmenting each of the stained images into multiple segmented regions in a predetermined segmentation mode (step S13), acquiring a uniformity level in each stained image by using a trained model generated by machine learning, the uniformity level indicating uniformity of a stained condition of each of the segmented regions (step S14), and determining suitability of each of the segmented regions for a predetermined analysis method in accordance with the uniformity level in each of the stained images (step S15). This allows accurate presentation of an analysis matching region that is common among the stained images.