54 results about "Stain method" patented technology

This invention provides a PAM staining method that enables staining suitable for magnified observation of various samples using a range of microscopes, from optical microscopes to electron microscopes. [Solution] A PAM staining method comprising the steps of: silvering a biological sample; coloring the biological sample; and fixing the color of the biological sample, wherein the biological sample is a biologically derived sample that has been oxidized; the silvering step involves immersing the biological sample in a methenamine silver aqueous solution at room temperature, raising the methenamine silver aqueous solution from room temperature to 62°C or higher and 68°C or lower, and carrying out the silvering reaction for 30 to 40 minutes from the start of the reaction at room temperature; the coloring step involves immersing the silvered biological sample in a 0.2% gold chloride aqueous solution at room temperature for 8 to 12 hours; and the fixing step involves immersing the sample in a hard film fixing solution containing 11% to 13% sodium thiosulfate and aluminum potassium sulfate dodecahydrate for 3 minutes or more at room temperature.

The application discloses a kind of for carcinosarcoma evaluation section and its preparation method and application, belong to pathological technical field.The preparation method includes the following steps: reticular fiber staining: silver staining method is carried out to the reticular fiber in section tissue and is dyed;Immunohistochemical staining: AE1 / AE3 protein in section tissue is marked and dyed by immunohistochemical staining method.The section obtained by the preparation method can present two kinds of staining on a tissue section, improve the expression effect of cancer, sarcoma, carcinosarcoma, reduce the identification difficulty, and be beneficial to tumor accurate typing.

The invention discloses a method for predicting the curative effect of triple negative breast cancer immunotherapy by spatially quantizing CD39 + CD103 + CD8 + T cells, and relates to the technical field of biology.The method comprises the following specific steps that S1, an FFPE tumor tissue sample of a TNBC subject before immunotherapy is obtained, and a continuous section with the thickness of 3-5 microns is prepared and attached to a positive charge glass slide; s2, staining the section by using a combination containing CD8 / CD103 / CD39 / Pan-CK antibodies through a TSA sequential staining method, and then re-staining cell nucleuses by using DAPI; according to the method, spatial quantitative analysis is performed on a specific CD39 + CD103 + CD8 + T cell subset, a percentage index of target cells in CD8 + T cells and an average distance index of the target cells and nearest Pan-CK positive cells in a cancer nest region are set, and the target cells are determined according to comparison between the two indexes and a preset threshold value. According to the method, whether a subject is a potential benefit crowd treated by an anti-PD-1 / PD-L1 immune checkpoint inhibitor or not is accurately judged, a standardized technical means is provided for clinically screening the potential benefit crowd for immunotherapy, the accuracy and effectiveness of triple-negative breast cancer immunotherapy are effectively improved, and invalid treatment is reduced.

The invention relates to a method for staining bacteria, comprising the steps of a) staining a bacteria sample by means of a solution of crystal violet, b) adding Lugol's solution to the stained bacteria sample, c) rinsing with distilled water, d) adding a solution of safranine-O and fuchsin in diethylene glycol monoethyl ether, and e) rinsing with distilled water, wherein the method is carried out substantially without alkanol.

The present application relates to the field of plant biotechnology, and particularly relates to a fluorescence staining method for pollen germination of Dendrobium nobile Lindl, which comprises five steps: collecting Dendrobium nobile Lindl flowers with a stigma, removing extra parts such as sepals and only keeping the stigma, trimming the two sides of the stigma into a horizontal shape, placing it into a pre-cooled fixing solution for fixation, and then dehydrating it with gradient alcohol; cutting the dehydrated stigma along the middle line of the stigma with a scalpel; placing the cut sample into a softener for treatment to soften the tissue; transferring the transparent sample into a staining solution containing fluorescent dye for staining in dark conditions; after the completion of staining, taking out the sample, fixing it on a glass slide, covering it with a cover glass, and observing the pollen germination of the Dendrobium nobile Lindl stigma under a fluorescence microscope. The method can clearly and accurately observe the pollen germination of the Dendrobium nobile Lindl stigma, and provides strong technical support for the related research of Dendrobium nobile Lindl and other plant materials with large stigmas.

This invention discloses a high-throughput automated immunofluorescence staining device and its staining method. The device includes a liquid injection motion module, a reaction chamber module, a supply and waste collection module, and a control system. The liquid injection motion module includes an X-axis beam and a pipetting and auxiliary material robotic arm, which are driven in parallel to achieve sequential operation. The reaction chamber module includes a temperature-controlled station, a low-temperature reagent storage unit, and an auxiliary material supply unit. The supply and waste collection module includes a waste removal and cleaning unit and an auxiliary material waste collection unit. This invention achieves a 96-station array through a temperature-controlled reaction station, combined with the dual-drive parallel operation of the pipetting and auxiliary material robotic arms, eliminating action waiting time and significantly improving throughput. The use of a closed semiconductor cooling component linked with an automatic flip-top mechanism ensures uniform reagent activity over a long period. A plate-mounted small vacuum pump drives a rigid pipeline with deep-hole waste suction, combined with high-flow-rate wide-area spraying, to thoroughly remove waste liquid and reduce cross-contamination. This achieves full-process automation, significantly improving staining quality and efficiency.

Staining devices and methods for automated systems are provided herein. A dyeing method includes disposing a substrate proximate a dyed surface to establish an overlap between the dyed surface and the substrate. The staining method further includes maintaining a gap between the stained surface and the substrate to define a chromosome product according to the overlap and the gap, where the chromosome product has one or more meniscus edges. The staining method further includes disposing a portion of the staining fluid over the staining surface and distributing the staining fluid over the substrate.

The invention relates to a wine stain treatment system based on stain characteristics and dual-wavelength parameter matching, and the system comprises a data collection module which is used for collecting a wine stain region parameter, a patient skin parameter and a historical treatment parameter of a patient; presetting a dual-wavelength wine stain treatment method library; the color spot mapping module is used for extracting color spot characteristic parameters from the wine color spot area parameters and determining adaptive matching values of the color spot characteristic parameters and the dual-wavelength treatment methods so as to construct a characteristic mapping matrix; the matrix optimization and adjustment module is used for extracting a core influence factor and a weighting coefficient from the skin parameters of the patient and carrying out weighting operation and adjustment on the core influence factor and the weighting coefficient and the feature mapping matrix; the treatment method matching module is used for matching with a method library according to the feature mapping matrix; and the color spot treatment module is used for setting a dual-wavelength light source to perform color spot treatment according to the acquired dual-wavelength treatment method. Compared with the prior art, the method has the advantage that the safety and the personalized adaptability of a spot treatment scheme are improved.

The use of AST-3424 in the preparation of a drug for treating a patient with hepatocellular carcinoma. A pathological paraffin block or pathological section of ex-vivo liver tumor tissue from the patient with hepatocellular carcinoma is subjected to immunohistochemical staining to detect the protein expression level of an AKR1C3 enzyme, with the detection result showing an H-score greater than or equal to 200; or the pathological paraffin block or pathological section of ex-vivo liver tumor tissue from the patient with hepatocellular carcinoma is subjected to immunohistochemical staining to detect the protein expression level of an AKR1C3 enzyme, with the detection result showing that the sum of percentages of moderate-intensity staining and high-intensity staining is greater than or equal to 70%. The dosing regimen for AST-3424 is as follows: in each 21-day cycle, administration is performed on day 1 and day 8; the initial dose is 6.0 mg / m2 per administration; and the patient will be permitted to receive treatment for a maximum of 34 cycles.

A method for staining reticulocytes includes staining reagent A and stabilizing reagent B; stabilizing reagent B is used M seconds after staining reagent A is mixed with the sample; M is greater than or equal to 20 seconds; staining reagent A includes staining agent A10, adjusting salt C10, and water; staining agent A10 is neomethylene blue; the concentration of neomethylene blue in staining reagent A is 0.8 × 10⁻⁶. ‑4 g / ml ~ 8.0 × 10 ‑4 g / mL; adjusting salt C10 is used to provide a low osmotic pressure environment and a pH range of 7.0–8.0; stabilizing reagent B includes staining stabilizer B10, adjusting salt D10, and water; the staining stabilizer B10 in stabilizing reagent B includes aldehydes. A unique two-step reagent formulation and method are used to achieve rapid staining of reticulocytes in a step-by-step manner, achieving successful staining in just 20 seconds, and the staining effect can be maintained for a sufficiently long time, exceeding 120 minutes.

This invention relates to the field of immunoassay technology, specifically to a multicolor fluorescent diagnostic reagent for detecting PAP. The multicolor fluorescent diagnostic reagent includes Hurst dye, wheat germ lectin, fluorescein isothiocyanate, a counterstaining agent, an organic solubilizer, and a humectant. This invention incorporates multiple fluorescent dyes, enabling rapid and effective detection of glycoprotein signals in PAP patient specimens. Simultaneously, it clearly displays cell morphology in the specimens, allowing for timely exclusion of bacteria, fungi, and abnormal cells, thus helping doctors quickly and accurately determine the type of PAP in patients. Compared to the traditional PAS staining method, the multicolor fluorescent diagnostic reagent provided by this invention uses a one-step staining method, which is simple to operate, has a short detection time, and allows for rapid observation of test results, facilitating timely diagnosis and treatment for patients.

The present application relates to a kind of hematoxylin-eosin (HE) staining method and operation steps without xylene and alcohol, belong to pathological technique, apply in pathology, histology and clinical experimental diagnosis biomedical disciplines field such as embryo. The hematoxylin-eosin (HE) staining step of conventional paraffin section is at least 12 more additional steps before and after dyeing into dyeing liquid dyeing to mounting respectively needs to use including xylene and gradient concentration alcohol. Lengthy dyeing step is not only laborious and time-consuming, also pollutes air environment and harms personnel health. The present application is a kind of conventional paraffin section whole process hematoxylin-eosin (HE) dyeing step without using xylene and alcohol method and step, make its application in management HE dyeing process for improving pathological technical room work quality and efficiency, reduce reagent cost and eliminate environmental pollution is crucial.

The invention provides a dyeing method for removing autofluorescence of a single cell sorting micro-fluidic chip and a chip without autofluorescence. The dyeing method comprises the following steps: completely soaking the single-cell sorting micro-fluidic chip in a dye solution for dyeing until the top layer of the chip is dyed into a non-transparent black top layer, thereby obtaining the single-cell sorting micro-fluidic chip without autofluorescence, the dye in the dye solution is black dye; the single cell sorting micro-fluidic chip at least comprises a photosensitive dry film serving as a runner layer. The staining method provided by the technical scheme of the invention can effectively solve the problem of autofluorescence of a single cell sorting micro-fluidic chip prepared from a traditional photosensitive dry film, so that when the stained chip is applied to fluorescence activated cell sorting, recognition of automatically screened fluorescent cells is facilitated, the operation difficulty of fluorescence activated cell sorting is reduced, and the working efficiency is improved. And accuracy and reliability are improved.

To suppress the adhesion of stains to the outer peripheral surface of a roll caused by printing applied to a sheet, in an embossing unit.SOLUTION: An anti-fouling device 1 according to the present invention relates to an embossing unit 16 that forms embossing on a sheet 2 with printing by passing the sheet 2 between a pair of rolls 6A and 6B, and is provided with a storage section 1A and an oil supply section 1B. The storage section 1A stores oil for antifouling. The oil supply section 1B supplies oil stored in the storage section 1A to the outer peripheral surface of the target roll 6, which is at least one of the pair of rolls 6A and 6B.SELECTED DRAWING: Figure 2

The invention relates to the field of biotechnology and dead / living cell detection, in particular to a method for rapidly determining the activity of biological membrane cells in immobilized fermentation. According to the method, a methylene blue and trisodium citrate synergistic chromosome system is optimized, a biofilm EPS matrix is synergistically penetrated by virtue of the dispersion effect of trisodium citrate and the reduction characteristic of methylene blue, and a spectrophotometric method is combined to detect the OD value of a staining solution instead of stained cells, so that the efficient quantitative analysis of the ratio of dead cells to living cells of the immobilized biofilm is realized. The method can complete detection within 200 s, is simple and convenient to operate and low in cost, does not need complex equipment, and is remarkably superior to a traditional fluorescent staining method.

The invention belongs to the technical field of antibody detection, and particularly relates to a multi-subtype antigen co-transfected cell immunofluorescence staining method and application. The method is established for the purpose of a CBA detection method, the basic principle is similar to that of an overexpression antigen cell immunofluorescence method for existing anti-GFAP-IgG antibody detection, and the difference is that three GFAP subtypes are adopted for cotransfection design, antigen subtypes are covered, and the problem of leak detection is avoided. According to the invention, the high-quality expression of the antigen is realized by self-constructing the plasmid based on the pCDH lentiviral vector. According to the invention, by optimizing the schemes of fixation, transparent preservation and the like after cell transfection culture, the dyeing performance is enhanced, and the preservation effect is improved.

To provide an electronic staining method that enables electronic staining of even a resin like PAA which has an acidic functional group and is easy to elute into a reaction liquid, and can evaluate a distribution state of the resin.SOLUTION: There is provided an electron staining method for evaluating a distribution state of a resin by using an electron staining liquid, wherein the resin has an acidic functional group and the electron staining liquid consists of an inorganic compound and a polar organic solvent. Particularly, it is preferred that the resin is a polyacrylic acid having a carboxyl group, the inorganic compound is a cesium (Cs) compound, and the polar organic solvent is an alcohol.SELECTED DRAWING: Figure 1

The invention relates to the technical field of escherichia coli biofilm formation determination, in particular to a method for determining the escherichia coli biofilm formation capacity through a crystal violet staining method. The method comprises the steps of bacterial liquid preparation, microwell plate sterilization, inoculation and culture, washing and fixation, dyeing and dissolution and quantitative detection. The method comprises the following steps: systematically analyzing biological characteristics such as biofilm forming ability, drug resistance, drug resistance genes and virulence genes of avian pathogenic escherichia coli through a multi-dimensional evaluation system, introducing a local isolated strain in Gansu province as a contrast, screening a strong biofilm forming strain in combination with ANOVA, and detecting the virulence genes through PCR (Polymerase Chain Reaction). The invention finds that the drug resistance rate of beta-lactams in a strong biofilm strain is as high as 82.3%, target data is provided for regional antibiotic control, meanwhile, a biofilm strength-virulence gene-drug resistance spectrum correlation model is established, the limitation of single phenotypic research is broken through, and the clinical treatment guidance value is improved.

To provide an improved verification method for verifying the performance of a fluorescence-based analysis system.SOLUTION: (a) negatively staining cells in a first portion of a standard sample comprising target cells expressing a target cell marker; (b) positively staining target cells in a second portion of the standard sample; (c) passing the first and second portions of the standard sample through an instrument to obtain a fluorescence measurement indicative of target cell concentration; (d) based on the fluorescence measurement obtained in (c), each diluted sample having a nominal concentration of target cells, each nominal cell concentration being greater than the concentration of target cells indicated by the fluorescence measurement of the negatively stained first portion in (a); Preparing a dilution series comprising a plurality of diluted samples, wherein the nominal concentration in each diluted sample is different from the nominal concentration in each of the remaining diluted samples; (e) passing the dilution series of (d) through the instrument and obtaining a fluorescence measurement; and (f) comparing the nominal cell concentration of (d) and the fluorescence measurement of (e) for each diluted sample to quantify the performance of the staining method in the instrument.SELECTED DRAWING: Figure 1

w The invention discloses a detection method for predicting efficacy and prognosis of immunotherapy for small cell lung cancer based on ATAD2 expression, and relates to the technical fields of molecular biology and cancer treatment. The method comprises the following steps: extracting a tumor tissue sample of a small cell lung cancer patient through surgical resection; Detecting the tumor tissue sample to obtain ATAD2 expression level, wherein the detection adopts an immunohistochemical staining method. According to the invention, the expression level of ATAD2 in tumor tissues of small cell lung cancer is quantitatively evaluated by immunohistochemical detection technology, and the molecular marker information is systematically correlated with the clinical and pathological characteristics of patients by combining Cox regression analysis and Logistic regression analysis, so that the comprehensive prediction of immunotherapy efficacy and patient survival time is realized.

The invention discloses an annona squamosa seed vigor grading detection method, and relates to the technical field of annona squamosa seed vigor detection, and the method comprises the following steps: 1, collecting a seed sample; 2, seed pretreatment; 3, measuring the constant weight of the seeds; 4, measuring the water absorption rate of the seeds; 5, modeling after detection, and detecting and recording multiple data of the annona squamosa seeds through a TTC staining method, a conductivity determination test and a germination test; and step 6, vigor grading standard formulation. According to the method, multiple annona squamosa seed groups are adopted for detection, and multiple data of annona squamosa seeds detected through the water absorption rate, the TTC dyeing method, the conductivity determination test and the germination test are recorded, so that the method can be more convenient and more accurate when modeling comparison is performed on the data of the annona squamosa seed groups. Therefore, when the method is used for detecting and grading the activity of the annona squamosa seeds, the method can be more accurate, and the accuracy of the method during detection is improved.

The invention discloses a dyeing method for vagina micro-ecology detection. The dyeing method mainly comprises the following steps: 1) taking female genital tract secretions, and directly diluting the secretions with a diluent to form a suspension; (2) coating the suspension liquid on a glass slide by using a pipettor; 3) drying and fixing the suspension on the glass slide; 4) immersing the fixed suspension liquid glass slide into an eosin dye solution, and washing the glass slide with running water; (5) carrying out gram staining on the glass slide; and 6) observing by using a microscope. The invention also relates to a staining kit. According to the staining method, under the condition that the advantages of a gram staining method in the prior art are reserved, simple and rapid detection of trichomonad vaginalis can be achieved, the trophozoic form and flagellum form of trichomonad vaginalis can be clearly displayed, the accuracy of trichomonad vaginalis detection can be improved, and the method is suitable for popularization and application. The comprehensive and accurate detection on the vagina micro-ecology is facilitated.

The invention discloses application of hyperoside to treatment of endometriosis, and aims to evaluate whether hyperoside has an anti-fibrosis effect when applied to a rat endometriosis model by taking fibrosis as a treatment target. The method comprises the following steps: constructing a rat endometriosis model through an autotransplantation method, dividing molded rats into a low-dose group, a medium-dose group, a high-dose group, a model group and a western medicine control group 4 weeks after successful modeling, and laparotomizing and taking out ectopic lesions after respectively taking medicines for 4 weeks. HE staining and masson staining methods are used for mutually proving the fibrosis degree, the expression conditions of fibrosis indexes a-SMA, Co-1, CTGF and Fn are detected in an immunohistochemical mode, and the anti-fibrosis effect of hyperoside is evaluated. As a result, hyperoside can prevent the development of fibrosis in endometriosis, and thus hyperoside can be used as a drug for treating or preventing fibrosis in endometriosis.

The invention discloses a coomassie brilliant blue staining solution as well as a preparation method and a staining method thereof. The coomassie brilliant blue staining solution comprises the following components: coomassie brilliant blue, sulfate, inorganic acid, an alcohol solvent, a polar amide solvent and water. The sulfate and the polar amide solvent are introduced into the coomassie brilliant blue staining solution; the sulfate can shorten the dyeing time and reduce the dyeing background; the polar amide solvent can improve the solubility of the coomassie brilliant blue staining solution, so that the staining efficiency and speed are improved, and the detection sensitivity is improved. The protein gel dyeing method is based on the improved coomassie brilliant blue dyeing solution, microwave heating is combined, the whole dyeing process of SDS-PAGE can be completed within 10 minutes, the steps of gel fixation, sensitization, decoloration and the like in a traditional dyeing procedure are omitted, the process steps are simplified, the dyeing time is saved, and the dyeing efficiency is improved. The method has the advantages of fast detection, high sensitivity and small background interference.

The present invention discloses a staining solution and a fading / color separation solution for a simple whole-pollen methyl green staining method. The staining solution is composed of methyl green, manganese chloride, manganese sulfate, ethanol, and acetic acid, and the fading / color separation solution is composed of triethanolamine, ethanol, and glycerol. Based on the selective binding of methyl green to DNA and the dual effects of triethanolamine in fading methyl green and transparentizing the pollen wall, the present invention simplifies the post-staining fading, color separation, and transparency processes into a single step. This method not only achieves clear contrast in the staining of pollen nuclei, but also provides an easily controllable staining effect with good repeatability. Furthermore, the method is simple and quick to operate, and has the advantages of safety, environmental protection, and low cost. The method can be used to observe and determine the developmental stage of pollen from cruciferous crops and other crops with similar structures and sizes. It is also expected to find applications in research fields such as plant classification, sterile line conversion, and gene expression during pollen development.

The present invention relates to the technical field of tissue section staining, and in particular to a multicolor composite staining kit, a staining method, and applications thereof. The multicolor composite staining kit provided by the present invention not only achieves good staining effects and has a high degree of differentiation in staining different tissue structures, but also does not contain hazardous chemical components such as picric acid, ethanol, and ammonia, greatly reducing the associated risks and costs during production, storage, transportation, use, and disposal. In addition, the use of the kit of the present invention can effectively simplify the operating steps and reduce the difficulty of operation. While significantly improving the repeatability of the staining results, it is more environmentally and operator-friendly, has lower costs, and has good application prospects.

The invention belongs to the technical field of medical detection, and discloses a staining reagent applied to acid-fast staining and a staining method. The dyeing reagent comprises a primary dyeing agent, a decolorizing agent, a counterdyeing agent and an accelerating agent, wherein the accelerating agent is prepared from the following components: lauryl sodium sulfate, triton X-100, hexadecyl trimethyl ammonium bromide, a Tris-HCl buffer solution, ethylenediamine tetraacetic acid, sodium chloride, potassium chloride, protease K and beta-mercaptoethanol. According to the dyeing reagent, the dyeing accelerant is introduced into a traditional acid-fast dyeing system, the component capable of specifically damaging the lipid structure of the cell wall of the Mycobacterium is utilized, the hindering effect of the lipid structure on the dye in the dyeing process is reduced, the binding efficiency of the dye and thalli is remarkably improved, and therefore the positive detection rate of acid-fast dyeing is greatly increased.

The invention relates to a method for evaluating drug sensitivity of a salivary gland malignant tumor organ with negative driver gene mutation. The method comprises the following steps: S1, collecting a salivary gland malignant tumor sample with negative driver gene mutation, cutting, digesting, filtering and centrifuging to obtain a salivary gland malignant tumor cell cluster; s2, mixing the salivary gland malignant tumor cell mass with matrigel, and adding into a salivary gland malignant tumor culture medium for culture after gelling to obtain a salivary gland cancer organ; s3, mixing and culturing the salivary adenosine carcinoma organs and the to-be-detected medicine, detecting by combining an ATP bioluminescence method and a living and death staining method, and calculating the tumor inhibition ratio according to the judgment standard that when the tumor inhibition ratio is greater than 50%, the detection medicine is sensitive, and when the tumor inhibition ratio is not greater than 50%, the detection medicine is sensitive to the salivary adenosine carcinoma organs; and when the tumor inhibition rate is less than 50%, the detection drug is not sensitive. According to the present invention, with the detection method integrating the ATP bioluminescence method and the live-dead staining method, the reliability of the result is substantially improved, and the effect of 1 + 1 > 2 is achieved.

The invention relates to the technical field of medical detection, in particular to an antibody combination for detecting spermatogenic cells, a kit and application of the antibody combination. The antibody combination comprises an antibody of a zinc finger transcription factor 4, an antibody of a conjugation complex protein 3 and an antibody of zona pellucida binding protein. Specific recognition and semi-quantitative analysis of spermatogenic cells of all levels in samples are realized through standardized pretreatment, full-automatic immunohistochemical staining and digital image analysis for different types of samples; the method breaks through the technical bottlenecks that the traditional HE staining or Wright-Giemsa staining method is strong in subjectivity, difficult to distinguish cells with similar forms and greatly influenced by cell states and staining effects, and provides an efficient detection means for accurate pathological typing, spermatogenic function evaluation and dynamic curative effect monitoring of male infertility.

The invention discloses a method for detecting sperm morphological anomaly through an improved Pasteur staining method, and relates to the technical field of morphological analysis, and the method comprises the following steps: carrying out staining treatment on a sperm smear by using the improved Pasteur staining method to obtain a training data set and a test data set; based on a U-Net network, designing a convolutional neural network VCA-Net applied to sperm morphological anomaly detection; based on the training data set and the test data set, performing model training and testing on the designed convolutional neural network VCA-Net to obtain a final convolutional neural network VCA-Net; and evaluating the final convolutional neural network VCA-Net by using the evaluation index to complete the anomaly detection of the sperm morphology by the improved Pasteur staining method. The problems that in the prior art, SMA automatic detection is difficult to achieve, and manual detection consumes time and labor and is large in workload and high in subjectivity under existing microscopic imaging are solved.