324 results about "Stain method" patented technology

The invention discloses a method and an automatic instrument device for enriching and extracting target cells and separating single cells by using a positive magnetic bead method and performing immunity and molecular biology identification and analysis on single cells. By the method and the instrument device, the on / off of a capture magnet and a release magnet is controlled by various methods to complete target cell searching, capturing, cleaning and releasing operation once or for multiple times, so that the target cell detection sensitivity and stability are improved. When the capture magnet searches and captures the target cells, the search line is circular, square, comb-shaped, S-shaped or U-shaped. In addition, the captured substances are filtered, most free micro magnetic beads are removed, the purity of the product is further improved and the product can be used as a good experimental material. By combining a special filter and the adsorption of the capture magnet, more than 95 percent of free micro magnetic beads can be effectively removed. Meanwhile, the types of the single cells are identified and biological characteristics of the single cells are analyzed by an immunofluorescence staining method and a method in molecular biology, and effective biological indexes are provided for clinical diagnosis and treatment of cancers.

The invention relates to an immunofluorescence staining method for suspension cells. The immunofluorescence staining method for the suspension cells is characterized by comprising the following steps of: collecting cells into a centrifuge tube; performing 800-gram centrifugation to collect the cells; respectively adding paraformaldehyde to fix, Triton-X100 to pass through and 1 percent bovine serum albumin (BSA) to close; adding a primary antibody and a secondary antibody sequentially to incubate; washing by an 800-gram centrifugation method after the operation of each step; performing diamino phenyl indole (DAPI) staining; and dripping on a glass slide and closing the glass slide to observe. The immunofluorescence staining method for the suspension cells has the advantages that: the immunofluorescence staining process is performed in the centrifuge tube, so the problems that the suspension cells grow on the glass slide difficultly and drop off easily in the test process are solved; the staining result is good; and the method is suitable for the suspension cells and cells which are adhered to the wall difficultly.

Disclosed are: a novel orange-colored disperse dye which has excellent staining properties onto a hydrophobic fiber material, a high light-resistant color-fastness, an excellent sublimation fastness, and a good buildup property, and is useful as a dye for use in an automotive interior sheet; a disperse dye composition for yellow-to-orange, brown, dark blue and black colors which comprises the disperse dye; an ink for ink-jet printing; a staining method or a stained material using the disperse die, the composition or the ink. The orange-colored disperse dye is represented by the formula (1) below and can stain a hydrophobic fiber material (e.g., a polyester) into orange color. [Chemical formula] (1) wherein X1 represents a chlorine or bromine atom; R1 represents a benzoyl group, a cyanoethyl group, a C1-C4 alkyl group, a phenyl group or a phenyl group substituted by at least one C1-C4 alkyl group.

The invention discloses a class of anti-tumor compounds containing a triazole heterocyclic structure and an application thereof, belonging to the technical field of medicinal chemistry. The compounds containing the triazole heterocyclic structure introduce quintuple 1,2,3-triazole ring in the form of side chains through the amino condensation, bromination, nitrine substitution, link reaction and other reactions on the basis of naphthalimide parent structure, select tetrazolium salt reduction to test 7721 hepatocellular in the experiment of inhibiting proliferation of tumor cells, and also select sulfonyl rhodamine B protein staining method to test MCF-7 breast cancer cells. The experiment results show that the anti-tumor compounds have wide anti-tumor activity and good inhibition effect on the normal growth of tumor cells derived from different tissues of liver cancer, breast cancer and the like.

The invention discloses a method for separating and appraising nucleated red blood cells in blood, including the following steps: (1) confecting reagent; (2) preparing anti-degumming slide; (3) selecting the staining method for nucleated red blood cells; (4) getting nucleated red blood cells from blood through microoperation. The method for separating and appraising nucleated red blood cells in blood can effectively identify nucleated red blood cells. Nucleated blood cell can be detected in 40 samples and the detection rate is 100 percent. Each sample is detected to have 2*106 to 6*106 / ml nucleated blood cell. In the invention, after the aniline being stained, the nucleated red blood cell is easy to be identified and skilled microoperation can quickly obtain the single nucleated red blood cell, so that the invention lays a good primary foundation to apply the single red blood cell in gene analysis.

The invention relates to a tissue dewaxing transparent agent free of benzene, which is used in biological histology, histopathology or forensic science, can make a tissue transparent, and can make the tissue dewaxed. The tissue dewaxing transparent agent belongs to the technical field of in vitro diagnostic reagents. The tissue dewaxing transparent agent mainly contains 95%-100% of alicyclic hydrocarbon, and the balance of accessories; the alicyclic hydrocarbon is a monocyclic alicyclic hydrocarbon or a bicyclic alicyclic hydrocarbon. The tissue dewaxing transparent agent free of benzene has the advantages of being non-toxic, soft in effect, and not easy to cause shrinkage, deformation, hardening and embrittlement of a tissue material, capable of improving the section intact rate, insensitive to the humid environment, not easy to muddy due to absorption of moisture in the air, and the like; not only can be used for liquid based cytology, immunohistochemistry, biological histology, routine pathological diagnosis, and immunohistochemical diagnostic techniques, is also suitable for special staining method and histochemical techniques. The refractive index of used alicyclic hydrocarbon transparent agent (cyclohexane) is 1.42, the (methyl cyclohexane) refractive rate is 1.42, and is basically consistent with the tissue refractive index (1.418), and the transparent effect is better.

The invention discloses a method for preparing nylon 66 colored and low-denier industrial yarns by a solution staining method. The method comprises the following steps: before spinning, adding color-different dyes, such as carbon black, and a dispersing agent polyethylene wax in a few of nylon 66 slices to prepare masterbatch; uniformly mixing the masterbatch with the raw material nylon 66 slices; and then carrying out spinning processes such as melting, spinning, drawing and winding, so as to prepare a finished product. In addition, the direct product of the preparation method is a colored product which normally needs to perform secondary processing for dyeing, and the secondary processing is high in pollution. The product prepared by the method is creative in China, does not need dyeing and reduces secondary operation.

The invention provides a staining method for aramid fiber. The staining method comprises the steps of preprocessing, preshaping, staining, reduction clearing, color fixing, soft processing, drying, shaping, calendaring and rolling. According to the staining method, DEET is added in to serve as a dye carrier, and the staining effect and efficiency are improved; meanwhile, substitute alkali is adopted to replace a conventional soda substance, and not only is the usage amount decreased, but also the color fixing effect is enhanced to enable the washability of the aramid fiber is further enhanced; the reduction clearing and color fixing processes are added, and therefore the staining fastness is good; green and environmental-friendly non-formaldehyde TCD-R is adopted in the color fixing process, the fiber is dried step by step after color fixing, and therefore the firmness of the fiber is greatly improved; the soft processing process is added, the color fastness and softness of fabric are improved, the roughness of the fabric is reduced, and therefore the fabric is fluffy and rich in elasticity.

The invention provides a hematoxylin-eosin mixed staining solution, which is characterized by comprising the following components: hematoxylin, an oxidant, a mordant, eosin, alcohol, weak acid, a preservative and water. The novel staining solution is prepared by mixing hematoxylin and eosin together; and cell and tissue samples only need one-time staining for staining both the hematoxylin and eosin. The present invention also provides a preparation method and a staining method of the hematoxylin-eosin mixed staining solution, and solves the problems of complicated operation, time consumption, false blue, unclear hierarchy and short retention time on the traditional hematoxylin-eosin staining.

The invention relates to a staining method suitable for blended fabrics prepared from polyester fibers, viscose fibers and modified polyester fibers. The staining method is characterized in that a one-bath one-step staining method is adopted for disperse dyes and cationic dyes, and comprises the following steps: (1) placing pretreated gray cloth into a dye vat according to a bath ratio of 1:10; (2) adding the disperse dyes; (3) adding a levelling agent at a use amount is 0.4-0.6g / L; (4) when a temperature is increased to 60-80 DEG C, adding the cationic dyes; (5) heating to 130 DEG C and carrying out heat preservation for 30 minutes; (6) cooling and washing with water; and (7) blocking the activity. The staining method provided by the invention shortens the process, increases the stand-alone output, and is featured by one-off heating, water washing and waste discharge so as to save large amounts of steam and water and reduce the wastewater discharge.

The invention discloses a method for molecular identification of Siniperca chuatsi basilewsky and Siniperca scherzeri Steindachner and a kit used by the same. The method for molecular identification comprises the following steps: DNA extraction, DNA fragment amplification, electrophoresis of PCR products and identification by a silver-staining method. The kit contains a pair of SSR primers and a standard map. The method for molecular identification can improve the efficiency and accuracy of identification of Siniperca chuatsi basilewsky and Siniperca scherzeri Steindachner, and is expected to solve the identification problem in the aquiculture of Siniperca chuatsi basilewsky and Siniperca scherzeri Steindachner and stimulate the sound development of Siniperca chuatsi culture industry.

The invention discloses a vaginal secretion staining fluid, and a preparation method and a staining method thereof. According to the preparation method, a blue basic dyestuff and a red basic dyestuff are mixed so as to obtain the vaginal secretion staining fluid, so that the vaginal secretion staining fluid possesses staining effects of universal staining fluid, staining time can be shortened greatly, staining time is shortened from general 5-30min to 20s, staining efficiency is increased greatly, and screening speed of clinical samples is accelerated. And in addition, imagines obtained via staining with the vaginal secretion staining fluid are clear, and are convenient for identification; surface layer squamous epithelial cells, middle layer epithelial cells, bottom layer epithelial cells, clue cells, leukocytes, erythrocytes, monilia albicans, and trichomonad can be identified clearly; and the vaginal secretion staining fluid is suitable for large-scale sample clinical screening.

The present invention relates to a staining kit and its preparation method, staining method and its application in several detection items for detecting canceration cell, red blood cell, campylobacteriosis, vaginal cleaning degree, estrogen level, garlandosus, trichomoniasis, mycosis, diplococcus gonorrhoeae and chlamydi trachomatis on a cervical smear. Said invented kit is formed from fixatino fluid, staining solution and differentiatino solution. Its staining method includes the steps of fixation, staining, differentiation and water-washing. Its film-making speed is quick, only has need oftwo main, and its detection accuracy rate is high, can be up to 98%.

The invention relates to an improved cervical squamous epithelial cell staining method, a cervical squamous epithelial cell staining kit and application of the method and the kit to diagnosis of individual cervical squamous epithelial cell cancers. The improved cervical squamous epithelial cell staining method comprises the steps of staining cervical squamous epithelial cells by using cell acid phosphatase staining reagent and staining the cervical squamous epithelial cells by using Pap staining reagent. The cervical squamous epithelial cell staining kit comprises the cell acid phosphatase staining reagent and the improved Pap staining reagent. The invention additionally relates to a kit for diagnosing individual cervical squamous epithelial cell cancers or precancerous lesions.

The invention discloses a target cell staining kit for displaying the proliferative activity of a cell and a use method thereof. The kit comprises I type and II type which respectively comprise an enzyme substrate, test paper, enzyme stationary liquid, phosphate buffer solution, Mayer hematoxylin and the like. The staining method comprises the steps of preparing pieces, fixing, staining and the like. The method can display acid phosphatase in exfoliative cells on smears such as exfoliative cervical cells, esophagus brush sheet cells and the like and search abnormal proliferated cells according to the target. Compared with the traditional Pasteur smear method, the method has the advantages of higher sensitivity for detecting cancer and precancerous lesions and similar specificity, therefore, the invention can be used for cytology screening of cervical carcinoma, esophageal cancer, lung cancer and other malignant tumors.

The invention discloses a coomassie brilliant blue staining solution and a staining method. The coomassie brilliant blue staining solution comprises 0.1-10 percent by volume of acid, 1-15 percent by volume of ethyl alcohol, 10-50g / L of soluble starch and 20-1000mg / L of coomassie brilliant blue aqueous solution. The staining solution does not contain reagents harmful to human bodies and is environmental friendly. The staining method using the staining solution has the beneficial effects that due to addition of the soluble starch, the staining background of gel can be reduced, a protein band can be specifically stained, the sensitivity of staining is effectively improved, and therefore, the whole staining process of the protein band can be completed within 20 minutes by using the staining method, the steps such as gel fixation, sensitization and destaining in a conventional dyeing process are omitted, operating steps are greatly simplified, and the dyeing time is saved; the staining method has the advantages of short cycle, high sensitivity and small background interference.

The invention discloses a floating sphere immunofluorescent staining method and a staining device, belonging to methods for coloring a sample for test. The staining method comprises the following steps: separation of spheres from a culture medium, fixing by stationary liquid, adding of Triton X-100 transparent cells, closing by confining liquid, adding of an interest protein-resisting primary antibody working solution, adding of a primary antibody-resisting fluorescent secondary antibody working solution, and cell nucleus staining by DAPI dye liquor. The staining device comprises a cell chamber and a staining sleeve part arranged at the outer part of the lower end of the cell chamber in a sleeving way. The floating sphere immunofluorescent staining method has the advantages of high efficiency, simpleness, convenience and the like, an antibody usage amount is reduced, experiment cost is reduced, manpower and time are saved, an accurate and reliable experiment result is obtained on the basis that a fundamental principle that immunofluorescent staining is not changed and specially training experimenters is not needed, sphere loss in an experiment operation process is avoided, an experiment success rate is increased, and the efficiency is improved.

The present invention discloses a staining method for cotton-silk elastic fabrics. The staining method mainly comprises scouring, bleaching, staining, finishing and other steps. The staining method for cotton-silk elastic fabrics provided by the present invention adopts a production process of continuous pad dyeing according to the characteristics of different raw materials, which can continuously execute production on a large scale, and ensures that the fabric surface of produced products is glabrous, beautiful, and soft in hand feeling. Therefore, the present invention can meet the requirement of consumers.

The invention provides a supercritical CO2 waterless staining dye pot and a waterless staining method. The supercritical CO2 waterless staining dye pot comprises a dye pot body and is characterized in that the dye pot body comprises at least two dye cylinders and a chassis, wherein each dye cylinder comprises a cylindrical shell and a top cover; the top cover is mounted at the upper end of the cylindrical shell; a metal mesh layer for placing of dyes is arranged at a lower part of the cylindrical shell, and a filter layer for filtering undissolved dyes is arranged at the upper part of the cylindrical shell; diversion holes are formed in the chassis; a rotary blocking piece for blocking the diversion holes is arranged on the bottom surface of the chassis; the lower end of the dye cylinder is mounted at a position of the diversion hole in the top surface of the chassis; inner cavities of the dye cylinders are respectively communicated with corresponding diversion holes. According to the invention, the phenomena of uneven color and staining in a waterless staining process are reduced; purposes that all dyes do not pollute each other and the amount of dye is controllable are achieved.

Provided are a full-automatic liquid-based cell preparation staining all-in-one machine and a preparation staining method. The all-in-one machine comprises a machine frame provided with a preparationcavity and a specimen cavity, multiple preparation cups, multiple specimen cups and multiple suction nozzles, and further comprises a specimen filling mechanical arm used for picking up suction nozzles and transferring specimens from the specimen cups to the corresponding preparation cups, a two-in-one mechanical arm used for filling staining solution to the preparation cups and discharging wasteliquid, a temperature control mechanism used for controlling the temperature of the preparation cavity and a control device. According to the method, the all-in-one machine automatically performs centrifugation and concentration, transfers the specimens to the preparation cups, and performs centrifugation preparation and centrifugation staining. The all-in-one machine achieves the automatic flow process operation of specimen concentration, sample adding, preparation and staining. The full-automatic liquid-based cell preparation staining all-in-one machine has the advantages of being applied tourinary system cancers screening, greatly reducing the artificial labor intensify in the urinary system cancers screening and reducing artificial error caused by urinary system cancers screening work, saving manpower resources, achieving the unification and standardization of urine liquid-based detection and preparation staining, and improving the detection rate of urine lesion screening.

The invention discloses a preparation method of a mangosteen shell natural dye, application and a staining method thereof. The preparation method of the mangosteen shell dye includes: subjecting a mangosteen shell to washing, air drying and mechanical crushing, then adding the crushed mangosteen shell into an ethanol water solution containing sodium hydroxide, performing reflux extraction at 60-90DEG C for 0.5-2h to obtain an extracted solution, then carrying out filtration and reduced pressure distillation on the extracted solution so as to obtain the concentrated mangosteen shell natural dye. For the ethanol water solution, the mass content of ethanol is 20-40%, per kilogram of the ethanol water solution contains 0.5-1.5g of sodium hydroxide, the crushed mangosteen shell and the ethanol water solution are in a mass ratio of 1:10-30, and the mass of the concentrated mangosteen shell natural dye accounts for 1 / 6 to 1 / 2 of the mass of the ethanol water solution. The extraction process of the mangosteen shell natural dye involved in the invention causes no environmental pollution. A dyed fabric has soft color and good color fastness, is safe to wear, does not have carcinogenic and teratogenic effects or cause allergic reaction. With good ecological environmental compatibility, the mangosteen shell natural dye is biodegradable.

The present invention provides assistance for visual observation based counting of particles or crystals. In an image of a specimen of an unidentified sample, particles are counted from an image obtained as a result of binarization using a method such as the discriminant analysis method, for example, and crystals are counted from two images using the difference between the images according with different imaging conditions. As an example, in the particle counting, such as a two-step noise removal is performed, and also, for the crystals, alignment and aspect ratio calculation are performed. In particular, the present invention can provide assistance for the dispersion staining method in which asbestos crystals are visually searched for using a phase-contrast microscope.

The invention relates to an ink coloring method for glued laminated bamboo. The method is as follows: the bamboo is removed of tabasheer, put into water to be steamed and boiled certain time, dried to certain moisture content, added into additional water, then added with antioxidant, insect-resist agents, anti-mildew agent, preservative and printing oil orderly to be steamed and boiled for certain time and dried to some extent, and then is immersed in glue, sprayed with glue, dried and pressed into glued laminated bambll. The glued laminated bamboo manufactured by the invention can make the ink permeate the bamboo fiber of the bamboo in order to be colored and then manufactured into various bamboo materials; meanwhile, the glued laminated bamboo manufactured by the invention is unlikely to change color or fade despite ultraviolet rays and weathering. Meanwhile, the sugar and organic minerals can be thoroughly removed, which enhances the insect-resistant, mold and corrosion resistant performance of the glued laminated bamboo.

The invention provides an eosin staining solution comprising the components of eosin, Biebrich scarlet, flame red, ethanol and water. The invention also provides a preparation method of the eosin staining solution, a HE staining solution containing the eosin staining solution and a staining method of the eosin staining solution. When the eosin staining solution provided by the invention is used for staining slices, the background is clear, the gradation is distinct, the staining effect is good, and the slices can be read easily, cannot fade easily and are convenient to store for a long time.

The invention discloses a culturing method of high-zinc nutrient high-grade rice, including: collecting rice material, planting and managing in a normal method, selecting beefy and plump rice material, originally screening by using the seed diphenylthiocarbazone staining method to obtain the material with red incision, preparing fine rice powder, determining the content of zinc in the rice, selecting the rice with the zinc content of over 25 mg / kg; shelling by a sheller, selecting the rice with transparency appearance and little chalkiness, testing the physical and chemical quality, selecting the rice with the amylase content of 15% to 25% , the jelly consistency of over 60 mm and the alkali spreading value of over 5 grade, continuing to plant and reproduce, evaluating the stability of high-zinc nutrition and high-grade inherited characteristic to obtain the high-zinc nutrient high-grade rice. The rice cultured according to the invention is nutrient and high-grade, has simple method and lower cost, and is convenient for the screening use in the breeding of large group.

The invention discloses a fluorescent staining method for evaluating sperm motility of domesticated mammals. The fluorescent staining method comprises the following step: according to the characteristic that a mammal live sperm probe Hoechst 333422 and a mammal dead sperm probe PI can be excited by the same ultraviolet light to emit fluorescent light, performing fluorescent staining on sperms of four important domesticated mammals which are pigs, cows, sheep and dogs to accurately perform sperm mobility evaluation. Compared with the conventional PI / SYBR-14 staining method, the PI / Hoechst 33342 staining method adopts Hoechst 33342 to substitute an expensive SYBR-14 probe, so that the detecting cost is greatly lowered. The fluorescent staining method can be used for successfully evaluating the sperm mobility of important domesticated mammals such as pigs, cows, sheep and dogs, and can be widely applied to evaluating the sperm motility of various domesticated mammals.

The present invention provides a protein and gamma-polyglutamic acid simultaneous coloring counterstaining method, which is an electrophoresis staining method belonging to the field of biotechnology, and can allow the protein on gel and gamma-polyglutamic acid to be chromogenic simultaneously. The method is characterized by adopting the following steps: (1) preparing protein and gamma-polyglutamic acid electrophoresis samples and performing polyacrylamide gel electrophoresis, (2) fixing the gel after electrophoresis, firstly staining with Coomassie brilliant blue R250 and bleaching, (3) placing the gel after the first staining in a methylene blue dye liquor for re-staining and bleaching until the protein and gamma-polyglutamic acid strips are clear. Compared with the prior art, the present invention solves the problem of simultaneous staining of protein and gamma-polyglutamic acid, can determine molecular weight and purity thereof according to the strip band position and the strip band number of the stained gamma-polyglutamic acid, and is convenient and fast to operate.

The invention relates to a acclimation direction adopted recycling small amount aborigine PHA high yield composition mushroom to do í«gage-restrictingí» acclimation primary activity sludge, force PHA composition mushroom to form dominance flora, and increase activity sludge composition PHA yield. Its purpose is to set aborigine PHA high yield composition recycling method, supply a simple activity sludge acclimation setting. Its operation steps are adopting Nile-Red fluorescent staining method to separate the PHA composition mushroom from the activity sludge; scalping PHA high yield mushroom as the recycling mushroom; its 0.1-0.25g are inoculate in reaction barrel to finish acclimation periods; discharging acclimation fluid; adding fermentation medium and microelement to do PHA ferment; collecting sludge deposition, drying, using chloroform to extract PHA; condensing extracted fluid in methanol to cement out PHA. This method can effectively increase activity sludge composition PHA yield from thirty percent to fifty percent.