174 results about "Liver tumor" patented technology

The invention provides a method for detecting alternative biological markers of liver neoplasms in saliva, serum and urine so as to detect expression information of alternative biological markers of liver neoplasms in a rapid, convenient and accurate manner. The method mainly comprises the following steps of: 1) preparing agglutinin chips corresponding to detection of saliva, serum and urine samples respectively; 2) pretreating stock solutions of saliva, serum and urine samples, removing impurities and purifying proteins, labeling by using a fluorescent reagent and removing redundant fluorescence by using G-25 columns to obtain corresponding samples to be detected; and 3) respectively loading three samples to be detected onto corresponding agglutinin chips to obtain respective glycoprotein sugar chain spectrum in each sample to be detected, and obtain information of the alternative biological markers according to reaction results of any sample to be detected. By the method, the sensibility during detection is high and detection results are accurate; moreover, the method has the advantages of safety, no damage, convenience for sample collection, simplicity in storage and the like.

The invention discloses a new traditional Chinese medicine composition for treating advanced malignant liver tumor symptoms and a preparation method thereof. The traditional Chinese medicine composition is mainly prepared from the following medicine components: angelica, tuckahoe, hemlock parsley, cassia twig, dried ginger, Bupleurum, kudzu root, Baikal skullcap root, coptis, flavescent sophora root, honeysuckle, Taraxacum, pangolin, leech, natural indigo, subprostrate sophora, moutan bark, red-rooted salvia root, red paeonia, white peony root, white atractylodes rhizome, gromwell, cornu antelopis powder, rhubarb, magnolia bark, three-nerved spicebush root, notoginseng, madder, draconis sanguis, rhizoma corydalis, raw Astragalus root, codonopsis pilosula, peach seed, safflower, turmeric, Endothelium Corneum Gigeriae Galli, Fructus Aurantii Immaturus, Polyporus umbellatus, rhizoma alismatis, Plantain seed, climbing fern spore, giant knotweed, Evodia rutaecarpa, nut grass, galangal and allium macrostemon. The traditional Chinese medicine composition can be prepared into any commonly-used oral preparation by using the conventional traditional Chinese medicine preparation method. The traditional Chinese medicine composition can be prepared into any commonly-used oral preparation by using the conventional traditional Chinese medicine preparation method. The invention can obviously improve the intense pain, obstructs the canceration of liver tumor cells, interdicts the reproduction of tumor cells, recovers the activity of liver cells, enhances the self-repairing function of the liver, resists the diffusion and prevent the metastasis, obviously shrinks the liver tumors, relieves and eliminates the ascites, improves the life quality, prolongs the life, relieves patient's pain and increases the appetite. The traditional Chinese medicine composition has exact and obvious clinical curative effect of improving the overall disease resistance.

The present invention relates to a system and method for patient specific modeling of liver tumor ablation. A method and system for tumor ablation planning and guidance based on a patient-specific model of liver tumor ablation is disclosed. A patient-specific anatomical model of the liver and circulatory system of the liver is estimated from 3D medical image data of a patient. Blood flow in the liver and the circulatory system of the liver is simulated based on the patient-specific anatomical model. Heat diffusion due to ablation is simulated based on a virtual ablation probe position and the simulated blood flow in the liver and the venous system of the liver. Cellular necrosis in the liver is simulated based on the simulated heat diffusion. A visualization of a simulated necrosis region is generated and displayed to the user for decision making and optimal therapy planning and guidance.

The invention discloses a puncture navigation method for CT guided liver tumor radiofrequency ablation. The method comprises the steps that markers which can be identified by CT are placed on the bodysurface of a patient suffered from liver tumor, and three-dimensional structures of the liver tumor, liver and key dissection structures (great vessels, lung, heart, stomach, kidney, gallbladder, skin and skeleton) are automatically and rapidly extracted from CT sequence scanning images in the CT guided liver tumor radiofrequency ablation; real-time automatic planning of puncture routes is conducted to obtain the optimal puncture route; spatial matching is conducted on a patient coordinate system P, a CT image coordinate system I and an electromagnetic positioning instrument coordinate systemE; information of the starting point, ending point, length, angle and the like of the optimal puncture route is displayed in CT image space; the moving of needle tip of an ablation needle is executedto reach the starting point of the optimal puncture route to conduct real-time navigation; after the needle tip of the ablation needle is moved to the starting point of the optimal puncture route, real-time navigation of the puncturing of the ablation needle is executed.

The invention discloses a new traditional Chinese medicine composition for treating min-term malignant liver tumor symptoms and a preparation method thereof. The traditional Chinese medicine composition is mainly prepared from the following medicine components: pangolin, red-rooted salvia root, tuckahoe, angelica, white peony root, red-rooted salvia root, peach seed, safflower, rhizoma sparganii, Rhizoma Zedoariae, giant knotweed, Chinese rrichosanthes root, coptis, flavescent sophora root, red paeonia, gromwell, orientvine, magnolia bark, Evodia rutaecarpa, Fructus Aurantii Immaturus, costus root, rhizoma cyperi, notoginseng, mastic, myrrh, draconis sanguis, Bupleurum, kudzu root, dandelion, madder, hyacinth bletilla, sanguisorba, ganoderma lucidum, ground beetle, agilawood, leech, vallicepobufagin, raw Astragalus root, ginseng and white atractylodes rhizome. The traditional Chinese medicine composition can be prepared into any commonly-used oral preparation by using the conventional traditional Chinese medicine preparation method. The invention can obviously improve the liver tumors and the symptoms of the abnormity of the liver function, the liver cell trauma, the hepatalgia, the hepatocirrhosis, the liver ascites and the like, obviously shrink the liver tumours, recover the liver function, activate the activity of the stem cells, regulate qi, remove the stasis, decrease the swelling, relieve the pain, enhance the body resistance to diseases, and obviously improve the life quality. The traditional Chinese medicine composition has exact and obvious clinical curative effect, high efficiency and quick action. By basically combining edible and pharmaceutical products specified in the national pharmacopoeia, the traditional Chinese medicine composition has the advantages of low cost, no toxic or side effects and the like.

The present invention provides compositions and methods featuring miR-26 microRNA polynucleotides for the diagnosis, treatment or prevention of hepatic neoplasia.

According to the liver tumor automatic classification method and device based on multi-stage CT image analysis, full-automatic bile duct cell carcinoma and hepatocellular carcinoma can be recognized,and a high-precision bile duct cell carcinoma and hepatocellular carcinoma recognition model is obtained. The method comprises the following steps: (1) acquiring a contrast-enhanced abdominal CT scanning image, storing the contrast-enhanced abdominal CT scanning image as an arterial phase, a portal vein phase and a delay phase, and carrying out definite diagnosis on liver cancer categories to which all data belong to serve as a model training gold standard; (2) constructing a three-dimensional full convolutional neural network segmentation model, and segmenting the intrinsic characteristics ofthe liver tissue in each stage from the abdominal CT image through model training learning; (3) constructing a three-dimensional convolutional neural network classification model; and inputting the image data obtained by segmentation into a classification model for training, so as to enable the model to perform joint learning and training on the cancer features in multiple periods, thereby predicting the category to which the cancer belongs, comparing the prediction result with a gold standard, and supervising the training process of the model in a loss value feedback mode.

A dual-particle tumor targeting system comprising a first ligand-mediated targeting nanoparticle conjugated with galactosamine and a second EPR-mediated targeting nanoparticle, wherein said first and second nanoparticles are mixed in a solution configured for delivering to a target liver tumor.

The invention discloses a tumor region segmentation method and system for a liver CT image based on a cascaded full convolutional network. According to the method, automatic segmentation of a liver tumor region is realized by using a full convolutional network model. The method comprises the following steps: performing preprocessing operations such as filtering and sharpening enhancement on a CT image; training a cascade network by using the preprocessed CT data set; achieving liver region segmentation by using two stages of FCN networks and segmenting a tumor region from a liver region of interest. A first-stage FCN network uses a variable pooling method so that more liver features are reserved and liver segmentation precision is improved; and a second-stage FCN uses hole convolution replaces an original convolution layer and a pooling layer, so that the position information of the target can be reserved while a image is reduced and the feeling domain is increased. A large number of features do not need to be manually extracted, and the tumor region can be effectively and accurately segmented from the liver CT image.

The invention relates to a liver-targeting intelligent nano-micelle prodrug system based on polyethylene imine, and provides a method for preparing a macromolecular prodrug. The low-toxicity polyethylene imine modified by polyethylene glycol with a liver-targeting group (T) at the remote end is used as a carrier (T-PEG-PEI), adriamycin (ADR) is used as a treatment medicament, and the carrier is connected with the adriamycin through a lysosome degradable covalent bond to form a prodrug (T-PEG-PEI-ADR). The T-PEG-PEI-ADR prodrug is self-assembled into micelles with grain diameter being 10 to 500 nanometers in water solution. The micelle prodrug system can circulate with long effect in blood, strengthen the phagocytosis of liver cancer cells on polymer nano-micelle, selectively deliver the treatment medicament to the liver tumor part, directionally kill the liver tumor cells, reduce the toxic and side effects of the adriamycin on treating the liver caner, and improve the clinical efficacy and bioavailability of the prior liver cancer treatment medicament.

The invention relates to a preoperative auxiliary device for thermal ablation of liver tumors, which is provided with a head-mounted VR display; a head-mounted in-ear earphone is installed on the head-mounted VR display; at least one locator; at least one control handle; and a locator , The control handle is wirelessly connected to the computer. The invention is more convenient to use, and doctors can design and plan different surgical plans multiple times with the assistance of the system, and perform simulated operations, select a more reasonable plan among them, and improve the success rate of the operation; compared with traditional two-dimensional image pictures , medical images in virtual reality are more realistic, doctors can diagnose and analyze patient image data from multiple angles, and at the same time, virtual reality technology can realize the interaction between doctors and image data.

The invention discloses a tanshinone IIA-polyactic acid / hydroxyacetic acid microsphere and a preparation method thereof. The microsphere is prepared by drying oil-in-water type emulsion, wherein the oil phase is dichloromethane solution of a polyactic acid / hydroxyacetic acid copolymer and the water phase is the water solution of polyvinyl alcohol. The drug content of tanshinone IIA in the microsphere is 1-10% and the entrapment efficiency is 60-90%. The particle size range of the microsphere is 30-200mm. The tanshinone IIA-polyactic acid / hydroxyacetic acid microsphere provided by the invention is suitable for interventional therapy of liver cancers, has a good liver tumor peripheral vascular thrombosis function, has an effective thrombosis time of 7-60 days, can be distributed in tumor tissues in a targeted manner, slowly release drugs, increase the local concentrations of the drugs, prolong the drug metabolism time, obviously inhibit animal liver tumor growth and prolong the animal lifetime and can inhibit expressions of a human hypoxia inducible factor 1alpha and a vascular endothelial growth factor and reduce the tumor tissue microvessel density after thrombosis.

Polypeptides whose expression is upregulated in liver tumor cells and cells from liver preneoplastic foci relative to expression in normal liver cells are disclosed as are polynucleotides that encode the polypeptides. In humans, the polynucleotide maps to a region of chromosome 15. The overexpression has also been confirmed in human liver, breast, colon and kidney cancer cell lines. It is believed that the polypeptides are overexpressed in tumor and preneoplastic cells in general.

The invention relates to the technical field of targeted drug carriers and preparations, in particular to a targeting carrier material and a targeting micelle preparation of liver tumors and preparation methods thereof. The targeting carrier material of the liver tumors is mainly formed by sequentially and covalently connecting angelica polysaccharide, glycyrrhetinic acid and antitumor drugs for the liver tumors, wherein the glycyrrhetinic acid and the antitumor drugs for the liver tumors are covalently connected through disulfide bonds. The micelle preparation comprises the targeting carriermaterial of the liver tumors and the antitumor drugs for the liver tumors, and the antitumor drugs for the liver tumors are encapsulated in the targeting carrier material of the liver tumors. The carrier material has reduction sensitivity, and can realize targeting selection on a tumor microenvironment with high reducing substances; the micelle preparation is formed by self-assembly of the carriermaterial and the antitumor drugs for the liver tumors; and the solubility and stability of hydrophobic drugs are enhanced by utilizing the characteristics of polymer-drug conjugates of the carrier material, and drug release can be carried out under the reduction environment.

The invention discloses a method for predicting the morphological change of a liver tumor after ablation based on deep learning. The method comprises the following steps: acquiring a medical image mapof a patient before and after liver tumor ablation; preprocessing the medical images before and after ablation; obtaining a preoperative liver region map and a preoperative liver tumor region map; acquiring a postoperative liver region map, a postoperative ablation region map and a postoperative liver tumor ghost image; obtaining a transformation matrix by using a CPD point set registration algorithm, and obtaining a registration result graph according to the transformation matrix; training the network through a stochastic gradient descent method to obtain a liver tumor prediction model; andpredicting the morphological change of the liver tumor of the patient after ablation by using the liver tumor prediction model. According to the method, the morphological change of the liver tumor after ablation of the patient can be predicted according to the CT / MRI image of the patient, a basis is provided for quantitatively evaluating whether the ablation area completely covers the tumor, a doctor can accurately evaluate the postoperative curative effect, and a foundation is laid for a subsequent treatment scheme of the patient.

The invention discloses a boundary-aware double-attention-guided liver segment segmentation method, which is called a boundary-aware double-attention-guided symmetric coding and decoding network and is used for accurately judging the position of a liver tumor. According to the method, the feature learning effect of the boundary in the medical image can be enhanced, and the segmentation precision of the liver segment is improved by accurately positioning the edge position. A double attention mechanism proposed by the method is composed of a space attention module and a semantic attention module in parallel. According to the invention, the low-level feature map with rich boundary position information is weighted from two dimensions of space and channel, and is spliced with the corresponding high-level feature map in the decoding path, so that the boundary feature expression is clearer and more prominent, the positioning of the liver segment boundary is facilitated, the segmentation accuracy is improved, and the liver segment segmentation problem is effectively solved.

The invention, which belongs to the medical equipment technology field, relates to a respiratory gating indication method used for radiotherapy and an apparatus thereof. The method comprises the following steps that: a remote controller that includes a CPU microprocessor, an arrangement key, control keys, a liquid crystal display, an infrared remote controlled coding circuit and an emitting device of the circuit is arranged; an infrared reception and decoding circuit, a second CPU microprocessor, an auxiliary LED indicating lamp and a nixie tube display are arranged; an indicator comprising a third CPU microprocessor, a drive circuit, a main LED indicating lamp and a sounder is arranged; and a power source is arranged. According to the invention, clear light and sound indication can be provided for a patient; therefore, an influence of a respiratory movement can be effectively reduced; localization precision of thoracic-abdominal tumor radiation therapy target region can be substantially improved; unnecessary irradiation on a normal organ and a normal part can be effectively avoided; and occurrences of phenomena of damage and necrosis of normal cells can be avoided or reduced. Besides, the apparatus is cheap and can be applied conveneitnly. Moreover, the method and the apparatus can be widely applied to radiation therapy of several kinds of tumors like a thoracic tumor, an abdominal tumor, a lung tumor, a liver tumor, a renal tumor and a breast tumor and the like.

The invention relates to nitric oxide donor OA or UA derivatives formed by coupling various types of NO donors and oleanolic acid (OA) or uraolic acid (UA) which is a medicament for treating liver diseases through ester bonds or amido bonds of link groups respectively. Bioactivity researches show that some compounds have the effects of killing liver tumor cells and have an application prospect in liver cancer treatment.

A method and system for tumor ablation planning and guidance based on a patient-specific model of liver tumor ablation is disclosed. A patient-specific anatomical model of the liver and circulatory system of the liver is estimated from 3D medical image data of a patient. Blood flow in the liver and the circulatory system of the liver is simulated based on the patient-specific anatomical model. Heat diffusion due to ablation is simulated based on a virtual ablation probe position and the simulated blood flow in the liver and the venous system of the liver. Cellular necrosis in the liver is simulated based on the simulated heat diffusion. A visualization of a simulated necrosis region is generated and displayed to the user for decision making and optimal therapy planning and guidance.

The invention belongs to the field of molecular biology (tumor screening), particularly relates to a technology for carrying out early warning and screening on liver tumor, and aims to solve the technical problem to provide a new choice for early prediction of the liver tumor. The technical scheme of the invention is that a kit for carrying out early warning and screening on liver tumor comprises a primer which is used for amplifying the following genes of an RASSF1A (Ras-association Domain Family 1A) gene of which a promoter is in a CpG island methylation state, a p16 gene, ELF (Embryonic Liver Fodrin), an SOCS (Suppressors of Cytokine Signaling)-3 gene, an SFRP1 (Secreted Frizzled-Related Protein 1) gene and a genome repetitive sequence LINE1 (Long Interspersed Nuclear Elements 1). According to the kit disclosed by the invention, a new effective inspection method is provided for carrying out early warning and screening on early liver tumor and liver cirrhosis; the cost is low, the use is very convenient, the flexibility is high, the specificity is good, and the application prospect is very good.

The No 95-281 peptide fragments of cell wither ligand (TRAIL) associated with recombinant tumor necrosis factor carried by adeno-associated virus carrier, its active form for expressing tripolymer in body, and its application in preparing medicines for treating transferred liver tumor and lung cancer are disclosed.

The invention provides a mouse liver tumor cell line for highly expressing CD133 and a preparation method thereof. The mouse liver tumor cell line with high content of CD133+cell subpopulations is established by the following steps of: importing a cancer gene Hras into p53- / - mouse fetal liver cells, obtaining a single-cell clonal group derived from CD133+cells by utilizing a monoclonal patternmaking technology, and finally screening. The cell line LPC-H12 expresses related genes of various liver stem cells and the stem cells, namely Marker:CD133 and EpCAM, and has high in-vitro balling capacity and in-vitro tumorigenic capacity. The mouse liver tumor cell line provides a powerful tool for researching the action and the mechanism of CD133+liver cancer stem cell subpopulations in the occurrence and development process of liver cancer and screening medicaments used for liver tumor stem cells.

The invention discloses nucleic acid for treating HCC (Hepatocellular Carcinoma), a preparation method thereof, a CAR-T (Chimeric Antigen Receptor T) cell having the nucleic acid and a preparation method of the CAR-T cell. A sequence of the nucleic acid at least comprises a CD226 costimulatory zone nucleic acid sequence as shown in SEQ ID NO.6 and a 4-1BB costimulatory zone nucleic acid sequence as shown in SEQ ID NO.7. An inhibition function of an HCC microenvironment can be overcome by promoting the generation of cell factors of IFN (Interferon)-gamma, so that the infiltration rate, the proliferation rate and the survival rate of T cells can be increased; after GPC3-226BB-CART cells are injected to a body, the GPC3-226BB-CART cells can survive in tumor and can be continuously proliferated until the tumor is removed.

The invention discloses a rat hepatoma cell strain with high metastatic potential, named rat hepatoma Wrh-f2 and collected in China Center for Type Culture Collection on 21th October, 2015 under CCTCC C2015174. The invention also provides a preparation method of the cell strain and an application thereof in the preparation of transplanted hepatoma models. In order to acquire cells with higher metastatic rate, a modified rat hepatoma induction method is used, tumor tissues which grow well are subjected to primary culture by means of a direct single-layer cell culture, rat hepatoma cells are obtained, a cell with high metastatic potential is screened by using a Transwell small chamber and is used to prepare a transplanted rat hepatoma model, and the analysis on liver tumor metastatic mechanism will be highly facilitated. In addition, purified cells are cultured by cloning, the cells with high metastatic potential and growing well are selected and collected, and a series of experiments show that the screened cell strain Wrh-f2 is a hepatoma cell strain having good nodule-forming characteristic.

The invention relates to a preparation method and of a liver cancer targeting glycoside ligand molecule and a preparation method of a liposome of the liver cancer targeting glycoside ligand molecule, and especially relates to a liver ASGPR target targeting glycoside ligand molecule and the liposome prepared by using the targeting glycoside ligand. The glycoside ligand molecule is formed by a liver targeting glycoside group, a lipophilic anchor and a substituted or non-substituted -(CH2)8 linear spacer arm for connecting the liver targeting glycoside group with the lipophilic anchor through an ester bond. The liver cancer targeting glycoside ligand molecule makes antitumor drugs in a drug delivery system maximally concentrated in liver tumor cells, improves the drug treatment index, reduces toxic and side effects on whole bodies, and improves patients' survival quality.

The invention discloses a culture medium and a culture method for constructing a liver tumor scaffoldless organoid. The culture medium comprises the following components: Advanced DMEM / F12, FBS, diabody, N-2, Noggin, B-27, EGF, FGF-10, Y-27632, A83-01, SB202190, R-Spondin, N-acetylcysteine, Nicotinamide, Wnt3A, HGF and PrimocinTM. The culture method comprises the steps of adding primary tumor cells separated and obtained into the above-mentioned special culture medium for resuspension, inoculating into an ultra-low adsorption U-shaped well plate after counting, and centrifugally culturing until organoids are formed. The culture medium and the culture method can realize in-vitro proliferation of primary liver cancer cells, can quickly construct the liver tumor scaffoldless organoids, and can maintain long-term stable growth of the liver tumor organoids. The tumor organoids formed by the culture medium and the culture method retain heterogeneity of tumor tissues of patients, and are suitable for drug sensitivity detection, tumor generation and development related research of in-vitro liver cancer chemotherapeutic drugs.

The invention belongs to the technical field of medicine. The invention discloses a norcantharidin liver-targeting liposome, a preparation method thereof and the application of a freeze-dried preparation. The norcantharidin liver-targeting liposome of the present invention is connected with a glycyrrhetic acid derivative on the surface of the liposome, which can be combined with the glycyrrhetic acid receptor on the surface of liver tumor cells and undergo endocytosis, thereby producing a tumor-suppressing effect. ; Norcantharidin is encapsulated in liposomes, which has better drug bioavailability and enhanced curative effect. The norcantharidin liposome freeze-dried preparation prepared by the invention has good stability, safety and reliability, is easy to store, and is beneficial to industrialized production.

The invention provides a novel biotherapy method for inhibiting primary liver cancer growth and metastasis. According to the method, miRNA-885-5p is adopted as a target point, a synthesized miRNA-885-5p stimulant is injected intratumorally, such that the growth of liver tumor is inhibited.

The invention relates to a Chinese medicament for treating liver cancer, which effectively solves the problems of poor treatment effect of the liver cancer and short life time. The Chinese medicament is prepared from zedoary, leech, radix pseudo-ginseng, Chinese lizardtail rhizome or herb, glabrous greenbrier rhizome, American ginseng, astragalus, coix seed, wall lizard and rhizoma corydalis. The Chinese medicament is prepared by the following steps of: crushing the zedoary, boiling the crushed zedoary, and collecting essential oil, aqueous solution and dregs for later use; including the essential oil with cyclodextrin, and crushing the essential oil into inclusion compound powder; crushing the radix pseudo-ginseng, the American ginseng and the rhizoma corydalis; performing reflux extraction with ethanol, filtering and collecting the ethanol, and concentrating the product into clear paste; merging dregs filtered from an extract of the radix pseudo-ginseng, the American ginseng and the rhizoma corydalis and dregs of the zedoary extracted with the essential oil, and mixing the combined mixture and the leech, the Chinese lizardtail rhizome or herb, the glabrous greenbrier rhizome, the astragalus, the coix seed and the wall lizard; boiling the mixture, adding the aqueous solution of the zedoary extracted with the essential oil, uniformly mixing, filtering and concentrating the mixture to obtain clear paste; merging the clear paste and the clear paste prepared from the radix pseudo-ginseng, the American ginseng and the rhizoma corydalis, drying the merged clear paste in vacuum, granulating, and mixing the granules and the inclusion compound powder. The Chinese medicament effectively inhibits the growth of liver tumors, and prolongs the life time of patients.