145 results about "Paraffin Block" patented technology

A formalin fixed paraffin block an organ is fixed on a stage, the stage is moved and a front end of a tubular blade is relatively positioned at a target part, the tubular blade punches the tissue, the tissue is extracted by a tissue extracting rod, a tissue block has been prepared in advance having a plurality of holes having inner diameter very close to the outer diameter of the tubular blade, the extracted tissues are planted in the holes on an embedding pan so that the front end of the tissue is protrusively facing toward the embedding pan, and liquid paraffin is filled into a gap between the embedding pan and the tissue block and solidified, whereby the tissue and paraffin are integrated, and good array block sections are constructed easily.

The invention discloses an FFPE reference product for gene detection and a preparation method and application of the FFPE reference product. The preparation method comprises the following steps that S1, cell culture is performed on a tumor cell line, and cell pellets are collected; S2, formalin fixation is performed on the cell pellets, then sepharose gel wraps the cell pellets to form cell aggregates, and the cell aggregate are prepared into cell paraffin blocks; S3, genomic DNA of the tumor cell line in the cell paraffin blocks is extracted, and genetic mutation frequency determination is performed on the genomic DNA of the tumor cell line; S4, the genomic DNA of the tumor cell line containing a target mutation site is mixed into a DNA mixture of a target mutation frequency, and the DNAmixture is the FFPE reference product for gene detection. According to the technical scheme, formalin fixation and paraffin wrapping are performed on the cells cultured by tumor cell line, so that thesituation of clinical samples can be better simulated.

The invention discloses a detection method for the peripheral blood circulating tumor cell VEGF of a patient suffering from advanced colorectal carcinoma. CTC in peripheral blood of the patient suffering from advanced colorectal carcinoma is obtained by conducting separation through a membrane filtration device; a thin cell layer is manufactured through the cell paraffin block technology; the VEGF gene expression situation of the patient suffering from colorectal carcinoma is detected. By means of the technical method, the VEGF expression situation of the patient where tissue samples can not be obtained can be detected without using colorectal carcinoma tissue. The technology is minimally invasive, and real-time detection can be conducted.

The invention provides an automatic cell paraffin block preparation device and method. The automatic cell paraffin block preparation device comprises a rotary table, a test tube, a semipermeable membrane, liquid taking devices, an extraction device and a pressing device, wherein the test tube is fixed in the first fixing hole in the rotary table and comprises a first tube body and a second tube body; the first tube body and the second tube body are detachably connected with each other; a hole is formed in the bottom of the second tube body; the semipermeable membrane is arranged between the first tube body and the second tube body; the liquid taking devices are used for sucking cell liquid, reagents and liquid paraffin; each liquid taking device comprises a mechanical arm, a suction pipe, first guide tubes and working pumps; the extraction device comprises a fixed part and a second guide tube; the fixed part adopts a hollow structure; an opening in the top of the fixed part is fixedly connected to a hole in the bottom of the second tube body; an opening in the bottom of the fixed part is fixedly connected to one end of the second guide tube; the pressing device is connected to the other end of the second guide tube; positive pressure or negative pressure is provided for the bottom of the test tube through the second guide tube.

The invention provides a porous element preparation method. The method comprises the steps of obtaining raw materials comprising, by mass, 50-75% of kieselguhr, 0-10% of aluminum oxide, 5-35% of a pore-forming agent, 5-10% of clay and 5-15% of glass powder; evenly mixing the raw materials with paraffin to prepare a paraffin block as a raw material; pressing the paraffin block as the raw material to form a required shape, and obtaining a green compact; conducting heat preservation on the green compact at the temperature of 200-500 DEG C for 4-10 hours, conducting sintering at the temperature of700-1200 DEG C for 2-4 hours, and obtaining a porous element. Compared with a common ceramic rod, an electronic cigarette with the porous element prepared by using the method easily and quickly emitssmoke, and the amount of the smoke is relatively large.

The invention discloses a preparation method of titanium dioxide nano powder, comprising the following steps: heating solid paraffin to a molten state, dropwise adding titanate into molten paraffin, fully and evenly stirring, cooling down to room temperature to obtain paraffin blocks dispersed with titanate, putting the paraffin blocks dispersed with titanate into water, heating and stirring, slowly releasing the titanate with the melting of paraffin, hydrolyzing the titanate in water medium to form dispersive and even emulsion, stewing, cooling and layering, taking out paraffin coagulums, centrifugalizing the solution, and calcining the obtained product in a retortfurnace to obtain the nano titanium dioxide powder. The method utilizes the solidification and melting of paraffin to controlthe whole reaction process, does not need to add any other organic surface active agent and dispersing agent, has the advantages of simple and easy control, green earth and repeated use of paraffin, and is particularly suitable for continuous production. The prepared product has high purity, even microcosmic appearance and size and good dispersity.

The invention discloses a method for detecting an ER gene of peripheral blood circulating tumor cells of a patient suffering from advanced breast cancer. The method comprises the steps of conducting separation with a membrane filtration device, so that CTC in peripheral blood of the patient suffering from advanced breast cancer is obtained; identifying the peripheral blood circulating tumor cells of the patient suffering from advanced breast cancer through the cellular immunofluorescence technique; preparing thin layer sections through the cell paraffin block technique; further detecting the ER expression condition of the peripheral blood circulating tumor cells of the patient suffering from advanced breast cancer through the immunohistochemical technique. According to the method, CTCs are separated and enriched by means of the ISET technique, and the CTCs are identified through the cellular immunofluorescence technique, so that the difficulty existing in CTCs identification conducted through the pure ISET technique and false negativeness existing in CTCs identification conducted through the pure immunological detection technique are overcome. The technique is not high in equipment requirement, the method is easy to grasp, and real-time monitoring can be conducted. By means of the technical method, the ER expression condition of the patient suffering from advanced breast cancer can be detected without picking breast cancer tissues.

The invention discloses a preparing method for an olefin tissue core which includes the steps of customizing an olefin model, customizing a tissue array hole plate, customizing a mandrel, customizing a sampling needle, customizing the olefin block of the tissue chip array of a receptor, sampling the olefin block of a supplier, preparing the olefin block of the tissue chip of the supplier, etc. The preparing method is simple and easy with good experimental result.

The present invention provides a technology and a method for preparing a high-throughput exfoliated cell chip. According to the present invention, a cervix brush is adopted to collect cervix exfoliated cells of 26 cervical lesions patients and are immediately placed into a cell preservation solution, centrifugation is performed, the supernatant is removed, a cell immobilization solution is added to immobilize cell precipitate, dehydration transparency and dipping in wax are performed, a tissue chip instrument is adopted to prepare an exfoliated cell paraffin chip and an exfoliated cell paraffin block array, and paraffin slicing is performed to prepare the exfoliated cell chip; and the defect of the conventional cytology detection is overcome, and the prepared exfoliated cell chip can be used for pathological diagnosis, IHC, in situ hybridization and other molecular medicine researches.

The invention provides a PET/CT (Positron Emission Tomography/Computed Tomography) macroscopical digital information and pathological microscopic information matching method. A postoperative sample is reduced into an in-vivo state and is soaked into formalin; a cross section is formed between the tracer highest-ingestion layer surface in a PET/CT image and an adjacent anatomic landmark; the sample is cut apart from the middle in the cross section direction, and is soaked into the formalin; HE (Hematoxylin-Eosin) staining pathological sections are manufactured; a paraffin block region of an immune tissue chemical staining region in each HE staining pathological section is determined; the selected paraffin block region is fixed, and the paraffin section cutting is performed; and the parameter of the tracer high-ingestion layer surface in each layer of PET/CT image and the corresponding layer immunohistochemistry expression are analyzed. The PET/CT image information and immunohistochemistry information matching accuracy is improved; a tracer high-ingestion region and a histology region are accurately registered; and after the matching, the accuracy of the PET/CT on the detection of the in-vivo oncobiology information can be verified.

The invention provides a hydrothorax and ascite malignant cell embedding method. The method comprises the following steps: putting hydrothorax and ascite in a plurality of 50-ml centrifugal tubes respectively, adding analytically pure ethyl alcohol into the centrifugal tubes respectively, and uniformly mixing and standing for 2-4h to obtain sediments; sucking up moisture in the sediment, wrapping a specimen by using a fixing mold and putting into a material drawing box; dehydrating the obtained specimen with the ethyl alcohol, and transparentizing the dehydrated specimen by adopting a transparent agent-xylene; putting the transparentized specimen in a 70-DEG C constant-temperature box for wax dipping, wherein an embedding agent is paraffin wax; and embedding the specimen after wax dipping with paraffin in an embedding workstation, pouring the specimen and the paraffin into an embedding box, putting the embedding box into cold water to rapidly solidify the paraffin after the paraffin on the surface of the embedding box is solidified, taking the paraffin block out of the embedding box and storing. The method can greatly increase the positive rate of cast-off cells, and can be used in further diagnosis of molecules, genes, immunohistochemistry and the like.

The present invention relates to a composition for aggregating biological sample used for manufacturing paraffin block for microscopic observation of biological sample, a method for preparing paraffin block using the same, and a method for microscopic observation of biological sample using the paraffin block, and particularly to a composition for aggregating biological sample including the first composite containing at least one water-soluble polymer selected from the group comprising polyvinyl alcohol, polyethylene oxide, polyacrylamide, polyvinylpyrrolidon, polyacrylic acid, polyethyleneimine, polyamines, polyamideamine, and polydiallyl dimethylammonium chloride and distilled water; and the second composite containing at least one organic solvent selected from the group comprising alcohol, xylene, and acetone and at least one medical adhesive selected from the group comprising cyanoacrylate, fibrin glue, protein glue, polyurethane, and sealant containing PEG (polyethylene glycol), a method for preparing paraffin block using the same, and a method for microscopic observation of biological sample using the prepared paraffin block.

The invention belongs to the technical field of detection equipment in the pathology department, and particularly to a slicing device for sample manufacturing in the pathology department. Specific tothe problems that the slicing device for sample manufacturing in the pathology department cannot be used for continuous slicing, slicing accuracy is low, consumed time is long and preservation is improper after slicing, the invention provides a scheme as follows: the slicing device comprises a mounting rack, supports are welded with the tops of outer walls on two sides of the mounting rack, a workbench is fixed on the outer walls of the tops of the supports through screws, and a sample manufacturing box is fixed at the top of an outer wall on one side of the workbench through screws. By the sample manufacturing box, subsequent samples are convenient to distribute uniformly, a magnetic head and a magnetic slicing cutter head cooperate with glass slides, paraffin blocks can be simultaneouslycut into multiple groups of slices with uniform thicknesses, the need for tissue to be sliced step by step is avoided, meanwhile, time consumed by tissue cutting is shortened, a sample slice storagebox can keep the optimum temperature for tissue preservation, and the dyeing problem caused by non-uniform slicing due to tissue unfreezing and softening is prevented.

The invention discloses an intelligent cabinet body used for informatization storage management of medical pathological specimens. A file cabinet is arranged at the top end of the cabinet body, a pathological glass slide placement box storage layer is arranged at the bottom end of the cabinet body, and a pathological paraffin block placement box storage layer is arranged between the pathological glass slide placement box storage layer and the file cabinet. Work indicating lamps and drawer electronic magnetic padlocks are installed at the positions of the pathological paraffin block placement box storage layer, the pathological glass slide placement box storage layer and file placement layer lattices, serial number positioning is performed on each drawer lattice, after drawers are placed onthe corresponding drawer lattices, electrifying work is performed, the drawers are locked, and the drawers can not be pulled out when a management system of the intelligent cabinet body does not senda working instruction; when the management system sends the working instruction that some drawer needs to be taken out, the work indicating lamp of the drawer is turned on while the corresponding drawer is automatically unlocked, and an operator only needs to directly pull out the needed drawer according to indication of the work indicating lamp.

The invention relates to a dyeing method for observing anther development structure changes of plants. By use of the dyeing method, the problem of clearly observing of an anther wall tapetum and structure changes and an anther wall can be effectively solved. The dyeing method comprises the following steps: fixing an anther by using a mixed solution containing formaldehyde, glacial acetic acid and alcohol, performing dehydration by using alcohol, then treating the anther for three times in sequence in a mixed solution containing n-butyl alcohol and alcohol, and soaking the anther in n-butyl alcohol; then transferring the anther into a mixed solution containing n-butyl alcohol and paraffin, pouring out half of the mixed solution and adding an equal weight of paraffin every 3h, and finally, pouring out the mixed solution, embedding the anther into a paraffin block by using paraffin, and cutting the paraffin block into a paraffin belt; and coating gelatin on a glass slide, attaching the paraffin belt to the glass slide, baking the paraffin belt, performing dewaxing, then treating the glass slide once in alcohol and distilled water respectively, treating the glass slide with the anther in periodic acid, rinsing the glass slide by using distilled water, performing dyeing in a Schiff reagent, rinsing the glass slide by using distilled water, and covering the glass slide with another glass slide. The dyeing method provided by the invention is easy to operate, good in dyeing effect, and clear and accurate in observation of the anther development structure changes of the plants.

The invention relates to a phase-change heat storage device for greenhouse temperature control. The phase-change heat storage device comprises an aluminum alloy paraffin block (1), an aluminum alloy hanging paraffin box (2), a hanging support plate (3) and a heat insulation layer (4), the specification of the aluminum alloy paraffin block (1) is 390*190*190mm, a cavity shell of the block is made of aluminum alloys, paraffin is injected into the paraffin block through a packaging opening (7) and sealed, and the aluminum alloy paraffin block is bonded on the inner surface of the north wall of agreenhouse. The specification of the aluminum alloy hanging paraffin box (2) is 240*190*480mm, a cavity shell of the box is made of aluminum alloys, paraffin is injected into the paraffin box throughthe packaging opening (7) and sealed, the paraffin box (2) is placed on the hanging support plate (3) and tied on the upper hanging support plate (3) through a hemp rope (5), and the hemp rope is connected on the aluminum alloy box through a knob (6). The device is based on good phase-change latent heat characteristics of paraffin serving as a phase-change material, heat is absorbed and stored inthe daytime, excessive temperature caused by heat accumulation in the greenhouse is avoided, the temperature is reduced to gradually release heat in the nighttime, heat environments by needed by cropgrowth in the greenhouse are maintained, freezing damage to crops is avoided, renewable solar energy is sufficiently utilized, good indoor thermal environments are created, energy consumption is reduced, artificial heating is decreased, and greenhouse production benefits are improved.

The invention relates to a production method of a paraffin section for observing a flower bud differentiation process. The method comprises the following steps: plant sample material taking and fixing, dehydrating and transparent, waxing and embedding, paraffin block correcting and slicing, adhesive tape unfolding, and dyeing and sealing; the step of plant sample material taking and fixing comprises cutting a plant stem tip central tissue, wherein the plant stem tip central tissue is cut with a stem tip central point and a base line of a blade as an axis, wherein the cutting width of the plantstem tip central tissue in the direction parallel to the base line of the blade is 4 to 6mm, the cutting width in the direction vertical to the base line of the blade is 2 to 4mm, and the cutting width in the height direction is 5 to 7mm. According to the production method of the paraffin section for observing the flower bud differentiation process provided by the invention, a special material taking method is adopted, the growth state of the plant stem tip can be reflected truly and accurately, and a cytological perspective is provided for observing the plant flower bud differentiation process; compared with a traditional paraffin section fixing method, the fixing method disclosed by the invention reduces the situation that young and tender materials become brittle after being fixed. The method of the invention ensures the accuracy of observing and measuring the angle of a growth point of the stem tip, is more efficient than the traditional paraffin section, and the obtained sectionhas higher accuracy.

The invention discloses a modeling mould preparation method based on the paraffin characteristics, and paraffin for mould preparation. The preparation method comprises the following steps: (1) weighing a proper amount of wax, placing the paraffin in a crucible, melting the paraffin, pressing the molten paraffin into blocks by using a mould according to the volume of the mould, and cooling the paraffin blocks; (2) designing the three-dimensional graphs according to the CAD two-dimensional graphs of a required product; (3) performing automatic route planning by using software, carrying out processing imitation, processing the paraffin rectangular blocks by a milling bed into the required shape; (4) drilling holes in the blocks by a drilling bed so as to obtain the required V-process moulds; (5) after the V-process moulds are used, placing the moulds and the waste paraffin scraps generated during the process of milling bed processing into a special paraffin container, melting the paraffin, repeating the step (1), and rapidly manufacturing V-process moulds in different shapes in sequence. The prepared paraffin moulds can be recycled, thus the production cost is reduced, the space occupied by the waste moulds is saved, and the utilization rate of factory is increased.

The invention provides an FFPE reference substance as well as a preparation method and application thereof. The preparation method of the FFPE reference substance comprises the following steps: selecting a target cell line; culturing the cells of the target cell line to a predetermined concentration, collecting the cells, and carrying out formalin fixation to prepare a paraffin block; and slicingthe paraffin block to obtain the FFPE reference product. According to the method, the FFPE sample which is the same as a clinical sample is prepared by selecting a proper cell line and culturing, so that a reference comparison effect from nucleic acid extraction to a detection result can be provided for the clinical FFPE sample, a quality control effect is achieved for the whole process of FFPE sample detection, and the accuracy of the detection result is improved. The method is suitable for large-batch preparation of tumor cell strain FFPE reference substances containing specific gene mutation proportions, so that the problem that no full-process FFPE reference substances exist in internal quality control during research, development and clinical examination of products such as kits prepared by enterprises is solved.

A protective sheath for prolonged storage of pathology paraffin blocks. The sheath protects the paraffin block against gouging, scratching, denting, rodents, and insects. A pathology slide slides into a slot in the protective sheath and is held into place by retainers running on the interior face of the protective sheath. A paraffin block cassette accepts a paraffin block and slides into a sample block sleeve holder disposed on one edge of the sheath.

A window with built-in vertical type rotary glass comprises a window body arranged on a wall, a motor and a controller. A moving opening is formed in the middle of the thick glass arranged in the middle of the window body, a movable glass block is arranged in the moving opening, and the top of the movable glass block is connected with the driving end of the motor through an upper rotating shaft; the controller comprises a metal outer shell, a metal inner shell, an input power line, an output power line, an n-shaped insulating plate and an input paraffin block, and an input conductive sheet connected to the input power line and an output conductive sheet connected to the output power line are vertically and oppositely arranged in the metal inner shell. By means of the window with the built-in vertical type rotary glass, automatic aeration cooling can be carried out in fires, fire losses can be conveniently lowered, and the window is simple in structure, high in practicability, low in cost and high in controllability.