8 results about "Paraffin Block" patented technology

A tamper for making paraffin blocks for histology may include a pliable tamper head and a handle. The handle may be permanently or removably attached to the tamper by using screws, magnets, or the like. The tamper may be made from a pliable material, such as a silicone rubber, with improved thermal transfer and high latent heat. In use, the tamper may be warmed in advance to a working temperature by placing the tamper on a hot plate of the embedding station or on a dedicated smaller hot plate. Alternatively, the tamper may be heated with an electric resistive element, a thermoelectric device, or an electric induction coil.

The utility model provides a rotary type paraffin slicing device which comprises a bottom plate, a shell is fixedly connected to the upper surface of the bottom plate, a threaded rod is rotationally connected to the interior of the shell, a handle is fixedly connected to the outer wall of the threaded rod, a sliding block is in threaded connection to the outer wall of the threaded rod, a supporting shaft is fixedly connected to the outer wall of the sliding block, and the supporting shaft is fixedly connected to the outer wall of the sliding block. A slicing knife is fixedly connected to the outer wall of the supporting shaft, a sliding shaft is slidably connected to the interior of the sliding block, and a fixing assembly is arranged on the outer wall of the limiting block and used for fixedly clamping a paraffin block. According to the paraffin slicing device, the slicing knife can be driven to move through mutual matching of the handle, the shell, the threaded rod, the supporting shaft, the sliding block and the sliding shaft, so that paraffin blocks are in contact with the slicing knife, the paraffin blocks with different thicknesses and hardness can be supported to be sliced through the movable slicing knife structure, and the paraffin slicing device is suitable for various scientific research and clinical scenes.

The present invention addresses the problem of providing a laminate that is easily manipulated and results in little damage to an original paraffin block sample when a paraffin section sample is prepared, a production method for the laminate, a preparation method for a paraffin section sample, and a preparation method for a paraffin sample piece.　Provided is a laminate that includes a substrate, a first paraffin layer that is provided on at least a portion of the surface of the substrate, and a second paraffin layer that is provided on at least a portion of the first paraffin layer, the melting point T1 of a first paraffin that constitutes the first paraffin layer being higher than the melting point T2 of a second paraffin that constitutes the second paraffin layer.

The invention discloses a cell preservation method and application, and the cell preservation method comprises the following steps: obtaining a target cell, and fixing the target cell; wrapping the fixed target cells with gel; the gel contains carbomer, a protease inhibitor, a cell permeation agent, a preservative and a buffer component, and directly preserving the target cells wrapped by the gel or embedding the target cells in paraffin to obtain a cell paraffin block for preservation. The cells fixed by the method can be directly preserved for a long time at 4 DEG C without dehydration, transparency and embedding, the gel concentration is adjusted to be liquefied before use, and the fixed gel can be removed by centrifugation; or paraffin embedding can be carried out according to a general process after gel wrapping, and paraffin is sliced before use. Therefore, the cell protein can be stored for a long time without complete denaturation, and meanwhile, an antigen repair process is not needed during use, so that the time is saved, and the efficiency is improved.

A cooling device suitable for cooling paraffin tissue blocks with little or no moisture artefacts, as well as the use thereof to obtain good quality serial sections for microscope slides. The slides can be used in histology and anatomical pathology, for example. The cooling device may include a cold plate, a lid, and a sheet of insulating material over the cold plate.

This invention discloses a method and its application for continuously maintaining low-temperature and humid conditions on paraffin blocks of tissue to be sectioned. The method includes the following steps: providing a heat-insulating and moisture-humidifying container filled with crushed ice; inserting the paraffin blocks of tissue to be sectioned sequentially into the crushed ice; and finally covering the container completely with crushed ice, ensuring each block is fully enclosed within the ice; adding water to the heat-insulating and moisture-humidifying container to form an ice-water coexistence system; and covering the container with a lid and allowing it to stand to allow the temperature and humidity of the paraffin blocks to equalize. By completely enclosing the paraffin blocks of tissue to be sectioned within the ice layers of the heat-insulating and moisture-humidifying container and adding an appropriate amount of water to form an ice-water coexistence system, a microenvironment with constant temperature and suitable humidity is created. The provided method requires no special instruments or equipment, is simple and easy to implement, low in cost, and reliable. It enables continuous sectioning of tissue blocks without removing them from the worktable, significantly improving sectioning efficiency and quality, and is particularly suitable for demanding continuous sectioning requirements.

This invention discloses a method for preparing composite immunohistochemical cell quality control samples. The method disclosed in this invention is applicable to the preparation of immunohistochemical quality control samples targeting multiple proteins, exhibiting strong versatility. Furthermore, this method enables multi-level, multi-gradient controls within a single paraffin block, significantly reducing the cost and complexity of immunohistochemical detection, and has broad application prospects in clinical disease diagnosis.

This invention discloses a device and method for judging the dehydration state of tissue paraffin blocks based on hardness and temperature detection. A hardness detection module is used to detect the hardness value of the tissue paraffin block; a temperature detection probe is used to detect the temperature value of the tissue paraffin block; a data processing unit is connected to both the hardness detection module and the temperature detection probe, and stores a standard hardness database for various tissue paraffin blocks at room temperature when dehydration is moderate; the data processing unit receives real-time hardness values ​​from the hardness detection module and real-time temperature values ​​from the temperature detection probe, performs temperature compensation on the real-time hardness values ​​at the real-time temperature, and compares them with the standard hardness values ​​for the corresponding tissue types at room temperature in the standard hardness database to determine the dehydration state of the tissue paraffin block; a display unit is connected to the data processing unit to display the judgment result. This achieves an objective, accurate, and quantitative judgment of the dehydration state of the tissue paraffin block.