17 results about "Paraffin Block" patented technology

A tamper for making paraffin blocks for histology may include a pliable tamper head and a handle. The handle may be permanently or removably attached to the tamper by using screws, magnets, or the like. The tamper may be made from a pliable material, such as a silicone rubber, with improved thermal transfer and high latent heat. In use, the tamper may be warmed in advance to a working temperature by placing the tamper on a hot plate of the embedding station or on a dedicated smaller hot plate. Alternatively, the tamper may be heated with an electric resistive element, a thermoelectric device, or an electric induction coil.

The utility model provides a rotary type paraffin slicing device which comprises a bottom plate, a shell is fixedly connected to the upper surface of the bottom plate, a threaded rod is rotationally connected to the interior of the shell, a handle is fixedly connected to the outer wall of the threaded rod, a sliding block is in threaded connection to the outer wall of the threaded rod, a supporting shaft is fixedly connected to the outer wall of the sliding block, and the supporting shaft is fixedly connected to the outer wall of the sliding block. A slicing knife is fixedly connected to the outer wall of the supporting shaft, a sliding shaft is slidably connected to the interior of the sliding block, and a fixing assembly is arranged on the outer wall of the limiting block and used for fixedly clamping a paraffin block. According to the paraffin slicing device, the slicing knife can be driven to move through mutual matching of the handle, the shell, the threaded rod, the supporting shaft, the sliding block and the sliding shaft, so that paraffin blocks are in contact with the slicing knife, the paraffin blocks with different thicknesses and hardness can be supported to be sliced through the movable slicing knife structure, and the paraffin slicing device is suitable for various scientific research and clinical scenes.

The present invention addresses the problem of providing a laminate that is easily manipulated and results in little damage to an original paraffin block sample when a paraffin section sample is prepared, a production method for the laminate, a preparation method for a paraffin section sample, and a preparation method for a paraffin sample piece.　Provided is a laminate that includes a substrate, a first paraffin layer that is provided on at least a portion of the surface of the substrate, and a second paraffin layer that is provided on at least a portion of the first paraffin layer, the melting point T1 of a first paraffin that constitutes the first paraffin layer being higher than the melting point T2 of a second paraffin that constitutes the second paraffin layer.

The utility model relates to the technical field of calibration and positioning devices, in particular to a paraffin section calibration and positioning device which comprises a frame, a plurality of connecting rods, a gradienter and two clamping assemblies. The clamping assembly comprises a side plate, a spring, a pull block, a guide rod and a clamping plate; when the paraffin slicing machine is used, the two clamping assemblies are used for clamping the frame on the sample clamp, a doctor obtains the position A of the bubble by observing the position of the bubble on the gradienter so as to obtain the position of the transverse axis and the position of the longitudinal axis of the sample clamp, and when slicing is needed on another paraffin slicing machine, the frame can be clamped on the sample clamp on the other paraffin slicing machine, so that the paraffin slicing machine is convenient to use. The positions of the sample clamps are adjusted until the bubble position on the gradienter moves to the position A, at the moment, it can be guaranteed that the positions of the two sample clamps are consistent, the angle tangent planes of the sample clamps of all the paraffin slicing machines can be kept consistent, and waste of materials due to incomplete tangent planes of paraffin blocks is avoided.

This invention discloses a bone marrow cell paraffin block, its preparation method, and its application, belonging to the field of pathological detection technology. The preparation method includes the following steps: Red blood cell lysis: Bone marrow aspirate is taken, and a precipitate containing cells and sediment is separated. A red blood cell lysis agent is added to lyse the red blood cells, and a lysed precipitate containing unlysed cells and sediment is separated. Formation: The lysed precipitate is resuspended in formalin solution for fixation, the supernatant is removed, and the precipitate is resuspended in agar solution. The agar is cooled and solidified, and then fixed again in neutral formalin. Dehydration: The solidified agar block is dehydrated. Embedding: The dehydrated agar block is embedded in paraffin to form a paraffin block. The cell paraffin block prepared by this method can supplement the diagnosis when bone marrow smears or bone marrow biopsy samples are not ideally prepared.

The use of AST-3424 in the preparation of a drug for treating a patient with hepatocellular carcinoma. A pathological paraffin block or pathological section of ex-vivo liver tumor tissue from the patient with hepatocellular carcinoma is subjected to immunohistochemical staining to detect the protein expression level of an AKR1C3 enzyme, with the detection result showing an H-score greater than or equal to 200; or the pathological paraffin block or pathological section of ex-vivo liver tumor tissue from the patient with hepatocellular carcinoma is subjected to immunohistochemical staining to detect the protein expression level of an AKR1C3 enzyme, with the detection result showing that the sum of percentages of moderate-intensity staining and high-intensity staining is greater than or equal to 70%. The dosing regimen for AST-3424 is as follows: in each 21-day cycle, administration is performed on day 1 and day 8; the initial dose is 6.0 mg / m2 per administration; and the patient will be permitted to receive treatment for a maximum of 34 cycles.

The present invention relates to a slicing device for processing pathological paraffin specimens. A clamping mechanism and a slicing mechanism are provided inside the body. A spraying mechanism is provided around the slicing mechanism. The slicing mechanism includes a conical table and a slicing knife. The end face of the conical table is a right triangle. The slicing knife is arranged on the upper part of the conical table. The spraying mechanism includes a water tank, a water pump, an input pipe, a ground nozzle and a stand nozzle. The water pump is externally connected to an input pipe, and the input pipe is respectively connected to the ground nozzle and the stand nozzle. The slicing device for processing pathological paraffin specimens can accurately control the thickness of the slices, is stable and reliable during the slicing process, and is convenient for the clamping plate to clamp the paraffin block, and also avoids the paraffin block from being excessively squeezed and deformed. The present invention increases the sliding speed of the paraffin by spraying water, alcohol and surfactant to the lower part of the cutter, and also appropriately softens the paraffin to avoid serious cracking and damage of the paraffin caused by the blade, and can ensure the stability of the paraffin.

A device for embedding a tissue sample includes a tissue cassette (10) and a mold (20). The tissue cassette (10) includes a frame (120), a top cover (100) coupled to the frame (120), and a bottom cover (110) coupled to the frame (120). The mold (20) defines a recess (220). The top and bottom covers hold the tissue sample therebetween. The top and bottom covers are capable of moving from a first position to a second position with respect to the frame (120), and in the second position the bottom cover (110) extend beyond a bottom face (123) of the frame (120) and is separated from the frame (120) and the top cover (100); the tissue cassette (10) is mountable on the mold (20), and the recess (220) is configured to receive the bottom cover (110) in the second position, such that a paraffin block (90) obtained by dispensing and cooling molten paraffin in the mold (20) is detachable from the mold (20) while the bottom cover (110) is remained in the mold (20).

The invention discloses a cell preservation method and application, and the cell preservation method comprises the following steps: obtaining a target cell, and fixing the target cell; wrapping the fixed target cells with gel; the gel contains carbomer, a protease inhibitor, a cell permeation agent, a preservative and a buffer component, and directly preserving the target cells wrapped by the gel or embedding the target cells in paraffin to obtain a cell paraffin block for preservation. The cells fixed by the method can be directly preserved for a long time at 4 DEG C without dehydration, transparency and embedding, the gel concentration is adjusted to be liquefied before use, and the fixed gel can be removed by centrifugation; or paraffin embedding can be carried out according to a general process after gel wrapping, and paraffin is sliced before use. Therefore, the cell protein can be stored for a long time without complete denaturation, and meanwhile, an antigen repair process is not needed during use, so that the time is saved, and the efficiency is improved.

The utility model discloses a pathological specimen wax sealing assembly, which comprises a plurality of pathological specimen embedding boxes, a porous operation plate for bearing a plurality of pathological specimen embedding boxes, and a plurality of pathological paraffin blocks which are fixedly adhered to the bottoms of the pathological specimen embedding boxes, a plurality of through pathological paraffin block fixing openings are formed in the porous operation plate, pathological paraffin blocks are embedded into and axially penetrate through the pathological paraffin block fixing openings, and handles extending upwards are arranged on the two sides of the porous operation plate respectively. After pathological tissues are sliced, pathological specimen embedding boxes filled with human tissues are respectively placed on pathological paraffin block fixing openings, then a handle is lifted up to simultaneously immerse the pathological specimen embedding boxes and the porous operation plate into high-temperature paraffin liquid, and then the handle is lifted up to take out the pathological specimen embedding boxes. The paraffin sealing work of a plurality of pathological specimens is completed at the same time, the wax film effect finally presented by the paraffin sealing work completed through the device is uniform and attractive, and the paraffin sealing efficiency of the pathological specimens is high.

A cooling device suitable for cooling paraffin tissue blocks with little or no moisture artefacts, as well as the use thereof to obtain good quality serial sections for microscope slides. The slides can be used in histology and anatomical pathology, for example. The cooling device may include a cold plate, a lid, and a sheet of insulating material over the cold plate.

This invention discloses a method and its application for continuously maintaining low-temperature and humid conditions on paraffin blocks of tissue to be sectioned. The method includes the following steps: providing a heat-insulating and moisture-humidifying container filled with crushed ice; inserting the paraffin blocks of tissue to be sectioned sequentially into the crushed ice; and finally covering the container completely with crushed ice, ensuring each block is fully enclosed within the ice; adding water to the heat-insulating and moisture-humidifying container to form an ice-water coexistence system; and covering the container with a lid and allowing it to stand to allow the temperature and humidity of the paraffin blocks to equalize. By completely enclosing the paraffin blocks of tissue to be sectioned within the ice layers of the heat-insulating and moisture-humidifying container and adding an appropriate amount of water to form an ice-water coexistence system, a microenvironment with constant temperature and suitable humidity is created. The provided method requires no special instruments or equipment, is simple and easy to implement, low in cost, and reliable. It enables continuous sectioning of tissue blocks without removing them from the worktable, significantly improving sectioning efficiency and quality, and is particularly suitable for demanding continuous sectioning requirements.

The invention relates to the technical field of pathology, in particular to a combined strain cell positive control wax block and a preparation method thereof. The preparation method comprises the following steps: strain samples are placed in an inactivation stationary liquid for inactivation treatment to obtain an inactivated strain suspension, the strain samples comprise aspergillus, cryptococcus, talaromyces marneffei and mycobacterium tuberculosis, and the inactivation stationary liquid comprises ethanol and a formaldehyde solution; carrying out centrifugal treatment on the inactivated strain suspension to obtain a precipitate, dissolving the precipitate in an agar solution, and then carrying out solidification treatment to obtain an agar block wrapping the inactivated strain; and sequentially carrying out dehydration treatment and embedding treatment on the agar block to obtain the combined strain cell positive control wax block. According to the method, a large number of positive control wax blocks of various fungi and mycobacterium tuberculosis cells can be rapidly manufactured, the positive bacteria are more, the background is clear, interference is little, misjudgment is not easily caused, positive bacteria can be rapidly observed, and the method can be widely applied to positive control of various morphological experiments.

The invention discloses an organoid rapid paraffin embedding method, and relates to the technical field of organoid embedding. The method comprises the following steps: inoculating an organoid into matrigel, uniformly mixing, and adding a culture solution for culturing; then absorbing and removing the culture solution, cleaning, and fixing the organoid by using a fixing solution; removing the stationary liquid, cleaning, adding gel, and cooling at room temperature until the gel is solidified into gel blocks; and finally, dehydrating the rubber block, and embedding the rubber block in molten paraffin. According to the method, a smaller number (less than 200) of organoid can be fixed, the damage to the form / structure of the organoid during centrifugation or dissociation is avoided, and the dehydration time is greatly shortened, so that the number and structural integrity of the organoid in the paraffin block are ensured.

This invention discloses a method for preparing composite immunohistochemical cell quality control samples. The method disclosed in this invention is applicable to the preparation of immunohistochemical quality control samples targeting multiple proteins, exhibiting strong versatility. Furthermore, this method enables multi-level, multi-gradient controls within a single paraffin block, significantly reducing the cost and complexity of immunohistochemical detection, and has broad application prospects in clinical disease diagnosis.

The present invention describes equipment for mounting, sectioning, and staining samples in paraffin blocks. This equipment includes motors that move the paraffin block containing a histological sample to perform a sectioning with a blade, which is activated by an actuator. The blade is sharpened at both ends to allow for sectioning in both directions of the actuator's movement. The equipment of the present invention includes a compartment where a slide is placed to collect the sample, which falls by gravity after sectioning. It also incorporates a bidirectional pump for supplying or draining water into the sample collection compartment. The compartment has a heating element that raises the temperature of the water in which the sample is immersed to remove the paraffin before staining.The equipment for the purpose of the present invention has a plurality of containers for storing different types of reagents for staining the samples.

This invention discloses a device and method for judging the dehydration state of tissue paraffin blocks based on hardness and temperature detection. A hardness detection module is used to detect the hardness value of the tissue paraffin block; a temperature detection probe is used to detect the temperature value of the tissue paraffin block; a data processing unit is connected to both the hardness detection module and the temperature detection probe, and stores a standard hardness database for various tissue paraffin blocks at room temperature when dehydration is moderate; the data processing unit receives real-time hardness values ​​from the hardness detection module and real-time temperature values ​​from the temperature detection probe, performs temperature compensation on the real-time hardness values ​​at the real-time temperature, and compares them with the standard hardness values ​​for the corresponding tissue types at room temperature in the standard hardness database to determine the dehydration state of the tissue paraffin block; a display unit is connected to the data processing unit to display the judgment result. This achieves an objective, accurate, and quantitative judgment of the dehydration state of the tissue paraffin block.