1089 results about "Bone marrow" patented technology

Disclosed are methods of treating amyotrophic lateral sclerosis in a subject by administering a therapeutic MSC secretome product made by a method comprising culturing bone marrow-derived MSCs under conditions that include oxygen tension below 5% and a culture media with a pH below 7.

Disclosed are methods of treating complex regional pain syndrome in a subject by administering a therapeutic MSC secretome product made by a method comprising culturing bone marrow-derived MSCs under conditions that include oxygen tension below 5% and a culture media with a pH below 7.

The invention discloses an intelligent gradient extraction process and method for nutritional ingredients in yak bone marrow, and belongs to the technical field of bioactive substance extraction. The extraction method comprises the following four steps: gradient pressure supercritical degreasing, double-enzyme synergistic gradient enzymolysis, acid concentration gradient demineralization and temperature gradient gelatin extraction, and through the process coupling design of gradient pressure degreasing, buffer enzymolysis, graded acidolysis and membrane separation of gelatin, the high yield of the product in the previous stage is ensured; active structures of follow-up components are reserved to the greatest extent. Meanwhile, a sensing-decision-execution closed-loop system is constructed, real-time data sources such as NIR moisture sensing, online OPA detection and XRD in-situ analysis are integrated, technological parameters are dynamically adjusted through a multi-objective optimization algorithm, and global optimization of the yield, the purity and the energy consumption is achieved.

Disclosed are methods of treating traumatic brain injury in a subject by administering a therapeutic MSC secretome product made by a method comprising culturing bone marrow-derived MSCs under conditions that include oxygen tension below 5% and a culture media with a pH below 7.

The invention relates to the technical field of disease diagnosis, in particular to a sacroiliac joint lesion intelligent identification method and system based on MRI, and the method comprises the steps: data preparation and enhancement, multi-expert collaborative labeling, mixed 3D-2D model processing, verification and optimization, and deployment and interaction. Compared with the prior art that a scheme for detecting the sacroiliac joint lesion by adopting a single 3D CNN or 2D CNN model has the problems of inaccurate positioning of an anatomical structure and loss of inter-layer feature association, resulting in insufficient sensitivity and specificity, the method provided by the invention has the advantages that through an improved hybrid 3D-2D model architecture, spatial features are extracted by utilizing the 3D CNN and an inter-layer dependency relationship is modeled in combination with Transform, so that the sensitivity and the specificity of the sacroiliac joint lesion detection are improved, and the detection accuracy of the sacroiliac joint lesion detection is improved. Meanwhile, spatial pyramid pooling and a channel attention module are adopted to enhance the multi-scale feature extraction capability, so that the method has the advantages that the lesion detection sensitivity and specificity are remarkably improved, the bone marrow edema region can be more accurately recognized, and the misdiagnosis rate is reduced.

The present disclosure relates to methods of diagnosing or treating bone marrow disorders and acute leukemia by using a tumor-specific antigen selected from the group consisting of CD63, CD151, CD72, CD84, CD69 and CD109. Antigen binding proteins (ABPs), ABP-drug conjugates, and CARs targeting tumor specific antigens are also provided, as are methods of their use.

Disclosed are methods of treating infertility or increasing fertility of a female subject by administering a therapeutic MSC secretome product made by a method comprising culturing bone marrow-derived MSCs under conditions that include oxygen tension below 5% and a culture media with a pH below 7.

The invention provides a treatment-caused myelosuppression risk early warning method and related equipment, and is applied to the technical field of data processing. The method comprises the following steps: processing multi-modal data information of a target user to generate a target physiological state prediction factor; processing the serum marker feature information of the target user to generate myelosuppression influence factors; processing the living habit information of the target user to generate a myelosuppression risk adjustment factor; processing the myelosuppression influence factor and the myelosuppression risk adjustment factor to generate dynamic risk parameter information of the target user; and processing the target physiological status prediction factor and the dynamic risk parameter information of the target user based on a target large-dose methotrexate bone marrow suppression risk prediction model to generate a bone marrow suppression risk value and a bone marrow transplantation risk level.

A telescopic nail with rotational stability, where the female component is externally D-Shaped through a special burnishing process or a plastic deformation, and the male component nests in the form of the female. The male component is partially D-Shaped to lock the two components together for easy removal. The thread of the tapered head of the female component has multi-leads and is synched with the multi-lead thread of the proximal head on the male components. The number of leads and the length of the thread are adaptable to the child's bone anatomy in such ay there is no intrusion of the bone joint. The male and female are locked together as an assembled nail and inserted simultaneously in the bone intramedullary canal. In case of revision or hardware removal surgeries, the assembly nail components are removed simultaneously together.

The invention discloses a multiple myeloma bone marrow image recognition system based on computer vision, relates to the technical field of cell recognition, solves the problem that traditional manual microscopic examination is large in limitation, and accurately recognizes burr change segments by conducting angle analysis on the edge contour of a feature area, so that the accuracy of recognition is improved. According to the comprehensive proportion of the burr change section on the overall edge contour, reliably judging whether the to-be-detected cell is a tumor cell or not, and calibrating and displaying the tumor cell in a gray image; according to the mode, the obvious difference of the tumor cells and normal cells in cell nucleus morphology is effectively utilized, rapid and accurate identification of the tumor cells is achieved, a visual and clear result is provided for clinical diagnosis, doctors are assisted to accurately judge multiple myeloma in time, the diagnosis efficiency and the treatment effect are improved, mean value processing and radial ratio analysis are conducted on contour area gray values, and the diagnosis accuracy is improved. The contour region associated with the cell nucleus can be accurately locked from the to-be-detected cell and is calibrated as the feature region, and a key cell nucleus feature basis is provided for tumor cell recognition.

The invention belongs to the field of tendon repair in biomedical materials, and provides a gradient mineralized collagenous fiber membrane and a preparation method thereof. And vertically placing the collagenous fiber membrane at the bottom of the container, and adding mineralizing liquid to prepare the gradient mineralized collagenous fiber membrane. The prepared high-orientation collagenous fiber membrane comprises a gradient mineralization layer and a non-mineralization layer, the gradient mineralization layer comprises an I-type collagen component and a HAp component in spatial gradient distribution, and the non-mineralization layer is composed of the I-type collagen component. The GMA-Col provided by the invention has an aligned layered fibrous structure, shows enhanced mechanical properties in the alignment direction, and is beneficial to spatial continuous change of mechanical rigidity. The differentiation surface morphology, the mineral content, the rigidity and the wettability of the local area can influence the adhesion, proliferation and differentiation of cells to a certain extent, and the mechanical property of the healing injury part and the recovery of the animal movement function are remarkably enhanced by implantation of the bone marrow bone marrow bone marrow bone marrow bone marrow bone marrow bone marrow bone marrow bone marrow.

The invention belongs to the technical field of biotechnology and animal models, and discloses a double-person-derived mouse model for simulating a tumor immune microenvironment and a construction method and application thereof. The method comprises the following steps: firstly, pretreating NSG immunodeficient mice by adopting low-dose whole-body irradiation in combination with double-antibody targeted bone marrow depletion, and transplanting CD34 + hematopoietic stem cells from the same human donor to complete human immune system reconstruction; separating tumor primary cells, tumor-related fibroblasts and tumor vascular endothelial cells of the same donor, performing three-dimensional co-culture to obtain homologous human tumor organs, and performing in-situ inoculation to immune reconstruction mice to obtain a target model. The core defects of MHC mismatching, low immune reconstruction efficiency, poor tumor immune microenvironment simulation degree, low clinical consistency and the like of an existing model are overcome, and the method can be used for tumor immune treatment drug screening, microenvironment mechanism research and personalized tumor treatment scheme verification.

Disclosed are methods of treating Ehlers-Danlos Syndrome in a subject by administering a therapeutic MSC secretome product made by a method comprising culturing bone marrow-derived MSCs under conditions that include oxygen tension below 5% and a culture media with a pH below 7.

The invention discloses a gastric cancer postoperative chemotherapy dynamic intervention method and system based on time point matching, and relates to the technical field of postoperative intervention. Health state data of wearable medical equipment of a gastric cancer postoperative patient is collected; preliminarily dividing the stomach cancer postoperative chemotherapy cycle into a plurality of key physiological time points based on the symptom inflection points, and respectively matching an intervention core problem and a corresponding stomach cancer postoperative chemotherapy intervention scheme for the plurality of key physiological time points; and the postoperative intervention platform analyzes the time period health state data collected by the wearable medical equipment at set time intervals, analyzes the health state data to automatically identify gastric cancer postoperative symptom inflection points, and dynamically matches key physiological time points based on symptom inflection point results. The stomach cancer postoperative treatment disruption rate is reduced, the crisis inflection point recognition accuracy is greatly improved, and the bone marrow suppression early diagnosis rate is greatly improved; the early diagnosis rate of postoperative wound infection of gastric cancer is improved, unnecessary intervention is reduced, and crisis event treatment is accelerated.

The present invention relates to a method for preparing a mammalian vascular network or mature mammalian bone marrow organoid. Also provided is the use of the prepared vascular network or mammalian bone marrow organoid in the treatment of bone marrow-related diseases, in vitro generation of BMO or mammalian blood cells, as a model system for the pathogenesis of bone marrow-related diseases, and as a system for identifying and / or testing pharmaceutically effective compounds for the treatment or prevention of bone marrow-related diseases.

Porous-based bone implants with the integration of Triply Periodic Minimal Surface, TPMS, porous architectures are designed to support the growth and proliferation of bone tissue, bone marrow, and capillaries. This disclosure intends to reduce the adverse effect of conventional implants such as bone resorption over time, which is called the “stress shielding effect”. The “stress shielding effect” is caused by the mismatch between the implant and natural bone stiffness. Triply Periodic Minimal Surface, TPMS, porous architectures exhibit interconnected pore features. The interconnection of the porous network allows the TPMS to have a higher permeability than that of other porous structures, leading to more favorable nutrient transport. In addition, many physical characteristics of the TPMS structures including surface-to-volume ratio, pore size, elastic modulus, and fluid behaviors can be controlled precisely through mathematical manipulation. As a result, TPMS-based implants could be physical features, which are vaned based on different bone regions. In other words, the medical implants may exhibit non-uniform or gradient physical features, which can match the characteristic of trabecular and cortical bones. Therefore, TPMS-based implants could adjust the features to mimic neighboring bone regions. As a result, we can achieve medical implants, which have superior mechanical and biological responses, resulting in optimal cell growth and better medical treatment.

The invention relates to the technical field of medical informatization, and particularly discloses a blood disease intelligent diagnosis and treatment data processing system which adopts an image processing technology based on deep learning to perform cell detection on a bone marrow morphological image of a patient object so as to focus on a cell target in the image. The method comprises the following steps: acquiring a cell target, performing multi-level feature extraction on the cell target to capture superficial detail features and trunk features of the cell, and performing significant sparse interactive coding on the superficial detail features and the trunk features to obtain cell feature representation with higher distinction degree, thereby realizing intelligent identification of abnormal cells. Through the mode, the cell characteristics in the blood sample can be automatically identified and analyzed, and rapid detection and diagnosis of abnormal cells are realized, so that a doctor can be assisted to carry out faster and more accurate blood disease diagnosis, and the workload of the doctor is relieved.

The invention relates to a recombinant complement protein and application thereof in preparation of anti-leukemia drugs, and belongs to the technical field of biological medicines. The invention discloses a method for treating acute leukemia by single drug, namely injecting recombinant C1QBP protein, which has a remarkable effect on treating acute leukemia, can be used for remarkably inhibiting proliferation of leukemia cells in marrow, spleen and peripheral blood of a hematopoietic system and also inhibiting infiltration of the leukemia cells in extramedullary organs (skin and the like), so that the whole-body tumor load is reduced, and the curative effect of treating acute leukemia is improved. The lifetime of leukemia mice is obviously prolonged. The recombinant C1QBP protein is combined with a clinical first-line chemotherapy regimen for use, has a synergistic effect, has a stronger effect of inhibiting leukemia cell proliferation compared with a single chemotherapy drug, can significantly prolong the lifetime of leukemia mice, and plays a role in reducing toxicity and enhancing efficacy. The invention proves that the complement C1Q binding protein has the effect of treating leukemia for the first time, provides a preparation method and a drug combination scheme, and has relatively high clinical transformation prospect and application value.

The invention provides a tibial plateau prosthesis and a preparation method, and belongs to the technical field of medical instruments. The tibial plateau prosthesis comprises a tibial plateau plate and an installation assembly, the tibial plateau plate comprises a plateau plate, a connecting sheet layer and a porous structure layer, the connecting sheet layer is arranged on the upper end face of the plateau plate, and the porous structure layer is arranged on the upper end face of the connecting sheet layer through a connecting structure; a solid surrounding edge is arranged on the outer edge of the porous structure layer, a mounting groove for mounting a tibial plateau liner is formed in the lower end face of the plateau plate, and the mounting assembly is arranged on the upper end face of the connecting sheet layer and is suitable for being implanted into a tibial marrow cavity. The invention provides a preparation method of a tibial plateau prosthesis. The preparation method comprises the operation steps of material preparation, 3D printing, semi-finished product disassembly, heat treatment, machining, polishing, cleaning, packaging and the like. The connection structure enhances the stability of connection between the porous structure layer and the connection sheet layer, and reduces the probability of falling of the porous structure.

The present disclosure generally relates to compositions and methods for treating a disease or condition associated with insufficient opening of Cx43 hemichannels in osteocytes, preferably for treating cancer, cancer metastasis, osteosarcoma, osteoporosis, or osteopenia.

The invention provides an anti-tumor modification method of stem / progenitor cell-derived neutrophil, which comprises the following steps: extracting mouse bone marrow-derived Lin-progenitor cells and human umbilical cord hematopoietic stem / progenitor cells, modifying ER-Hoxb8 progenitor cells by gene editing, and inducing the ER-Hoxb8 progenitor cells into neutrophil; three modification strategies of chimeric antigen receptor CAR gene engineering modification, high expression MET gene modification and high expression of IFN-gamma while the TGFBR2 gene is stably knocked out are respectively adopted, and the anti-tumor phenotype of the neutrophil N1 is maintained. Compared with the prior art, the engineering neutrophile granulocyte has the following advantages that the engineering neutrophile granulocyte prepared by the method has the advantages of infinite sources, long-time preservation, safety, controllability, efficient and rapid preparation and the like; the engineered neutrophil can maintain the N1 antitumor phenotype and promote the antitumor function; the immunosuppression microenvironment of the solid tumor can be improved and the treatment effect can be improved by singly using or combining the immune checkpoint inhibitor.

The invention provides application of luteolin in prevention of bone marrow-type radiation sickness, relates to the technical field of biomedicine, and provides application of luteolin in preparation of drugs for prevention and treatment of bone marrow-type radiation sickness. The invention discloses an application of luteolin in prevention or treatment of bone marrow type radiation sickness and radioactive hematopoietic system injury, and finds that the hematopoietic system injury caused by ionizing radiation can be slowed down by administration of luteolin before irradiation, specifically, peripheral hemogram is increased, and bone marrow injury is slowed down.

The invention discloses a method for analyzing a mechanism of treating leucopenia by using a Shengbai oral liquid by using a network pharmacology mode, and the method is based on an HITS algorithm, and identifies key regulation and control nodes through hub and authority scores of nodes. An analysis result shows that PPBP, ALOXE3 and CCR1 are hub genes ranked in the first three, and PPBP not only has the highest hub score, but also shows the most significant expression change. In a bone marrow microenvironment, PPBP / CXCL7 may affect differentiation and maturation of hematopoietic stem cells and promote generation of specific types of leukocytes.

The utility model provides a bone marrow puncture needle capable of keeping stable during puncture, and belongs to the technical field of medical auxiliary tools. Comprising a bone marrow puncture needle body, a handle is arranged outside the bone marrow puncture needle body, an operator can hold the puncture needle through the handle so as to improve stability, and the dismounting assembly is used for dismounting the handle and connected with the bone marrow puncture needle body. According to the bone marrow puncture needle, an operator can hold the puncture needle more stably during puncture through the grip arranged outside the bone marrow puncture needle body, and inaccurate puncture or patient injury caused by hand sliding or shaking is avoided. By means of the design, the stability and safety of puncture operation can be remarkably improved, an operator feels more comfortable during holding due to the design of the limiting part at the bottom of the grip and the smooth part at the top of the grip, better hand supporting can be provided, and the stability and accuracy of puncture operation are further improved. The utility model discloses a novel structure.

The invention relates to an application of extracellular beta-glucan derived from aureobasidium melanogenum in preparation of a training immunity inducer. By establishing a mouse bone marrow derived macrophages (BMDMs) and zebra fish juvenile fish training immune activation evaluation model, the immunocompetence of extracellular beta-glucan (eBG) derived from aureobasidium melanogenum is evaluated, and it is found that the extracellular beta-glucan has a more remarkable training immune activation effect compared with beta-glucan (cBG) derived from cell walls, and the immunocompetence of the extracellular beta-glucan (eBG) derived from aureobasidium melanogenum is improved. It is proved that the extracellular beta-glucan (eBG) from the melanin-producing aureobasidium is an excellent training immunity inducer and has a good application prospect in the fields of feed addition of bred animals and disease immune prevention and control.

The invention relates to full-automatic bone marrow biopsy puncture equipment and an application method. The full-automatic bone marrow biopsy puncture equipment comprises an operation table, and a puncture module and a three-axis motion platform are arranged on the operation table; the puncture module comprises a vertical rod, a transparent pressing cover is coaxially fixed to the upper end of the vertical rod, and an annular movable groove is formed in the lower surface of the pressing cover; a cavity is formed in the hold-down cover; a cross rod is arranged in the cavity, a through hole allowing the vertical rod to penetrate through is formed in the center of the cross rod, and the inner diameter of the through hole is matched with the outer diameter of the vertical rod; a driving assembly is arranged in the cavity, a color ultrasound probe and an electric push rod are arranged at one end of the cross rod, and a biopsy needle, a suction unit and a lead screw assembly are arranged at the other end of the cross rod; a three-dimensional camera for collecting three-dimensional actions is arranged in the hold-down cover; the functions of hold-down color ultrasound scanning, three-dimensional image acquisition, human body hold-down, biopsy needle simulated puncture, biopsy needle automatic needle insertion and the like on a human body are integrated, and the problem that an accurate and automatic biopsy needle puncture scheme is difficult to implement on the ground is solved.

The application relates to a wrist joint prosthesis and relates to the technical field of artificial joint prostheses. The wrist joint prosthesis comprises: a carpal bone assembly for being fixed with one side of a hand-shaped bone, which comprises a carpal bone pad, a carpal bone support block embedded in one side of the carpal bone pad, a carpal bone printing column and a screw connected to the other side of the carpal bone support block, and a support block adjusting device arranged on the embedded side of the carpal bone support block, one end of the support block adjusting device being located in the carpal bone pad, and the carpal bone pad being provided with a locking piece for locking the adjusted position of the support block adjusting device; and a radius bone assembly for being fixed with a radius bone marrow cavity, which comprises a radius bone support, the radius bone support being arranged on the side of the carpal bone pad away from the carpal bone support block. The wrist joint prosthesis has the advantages of shortening the waiting time for surgery.

The present disclosure relates to methods of conditioning and / or depleting cell populations in the bone marrow of a subject in need of hematopoietic cell transplantation (HCT), comprising administering an antibody that binds to a target on endogenous hematopoietic stem cells (HSCs) in the subject. In a preferred embodiment, the target is CD 117 (c-kit).

This invention relates to the treatment of osteoarthritic pain, or pain associated with osteoarthritis, and particularly for LINK_TSG6 polypeptide for use in the treatment of osteoarthritic pain. Osteoarthritis is caused by the deterioration of cartilage in joints and is characterised by pain and a loss of mobility in the affected joints. Osteoarthritic pain results from multiple factors including bone marrow lesions, sub-articular bone attrition, synovitis, effusion and innervation of usually insensitive tissues. LINK_TSG6 is a short recombinant peptide comprising the LINK module of human TSG-6.

The invention relates to a multiple myeloma model with extramedullary lesion as well as a construction method and application thereof. The multiple myeloma model is obtained by injecting bone marrow mononuclear cells (BMMC) of a multiple myeloma patient into an hIL-6NSG mouse. The invention provides a new model of multiple myeloma with extramedullary lesion, which not only is beneficial to research on gene pathway and mechanism of the multiple myeloma, but also lays a foundation for research on tumor recurrence, preclinical drug screening, effect evaluation and the like, and has great significance in promoting disease healing.