670 results about "Bone marrow" patented technology

The invention discloses an intelligent gradient extraction process and method for nutritional ingredients in yak bone marrow, and belongs to the technical field of bioactive substance extraction. The extraction method comprises the following four steps: gradient pressure supercritical degreasing, double-enzyme synergistic gradient enzymolysis, acid concentration gradient demineralization and temperature gradient gelatin extraction, and through the process coupling design of gradient pressure degreasing, buffer enzymolysis, graded acidolysis and membrane separation of gelatin, the high yield of the product in the previous stage is ensured; active structures of follow-up components are reserved to the greatest extent. Meanwhile, a sensing-decision-execution closed-loop system is constructed, real-time data sources such as NIR moisture sensing, online OPA detection and XRD in-situ analysis are integrated, technological parameters are dynamically adjusted through a multi-objective optimization algorithm, and global optimization of the yield, the purity and the energy consumption is achieved.

Disclosed are methods of treating traumatic brain injury in a subject by administering a therapeutic MSC secretome product made by a method comprising culturing bone marrow-derived MSCs under conditions that include oxygen tension below 5% and a culture media with a pH below 7.

The invention relates to the technical field of disease diagnosis, in particular to a sacroiliac joint lesion intelligent identification method and system based on MRI, and the method comprises the steps: data preparation and enhancement, multi-expert collaborative labeling, mixed 3D-2D model processing, verification and optimization, and deployment and interaction. Compared with the prior art that a scheme for detecting the sacroiliac joint lesion by adopting a single 3D CNN or 2D CNN model has the problems of inaccurate positioning of an anatomical structure and loss of inter-layer feature association, resulting in insufficient sensitivity and specificity, the method provided by the invention has the advantages that through an improved hybrid 3D-2D model architecture, spatial features are extracted by utilizing the 3D CNN and an inter-layer dependency relationship is modeled in combination with Transform, so that the sensitivity and the specificity of the sacroiliac joint lesion detection are improved, and the detection accuracy of the sacroiliac joint lesion detection is improved. Meanwhile, spatial pyramid pooling and a channel attention module are adopted to enhance the multi-scale feature extraction capability, so that the method has the advantages that the lesion detection sensitivity and specificity are remarkably improved, the bone marrow edema region can be more accurately recognized, and the misdiagnosis rate is reduced.

Disclosed are methods of treating infertility or increasing fertility of a female subject by administering a therapeutic MSC secretome product made by a method comprising culturing bone marrow-derived MSCs under conditions that include oxygen tension below 5% and a culture media with a pH below 7.

A telescopic nail with rotational stability, where the female component is externally D-Shaped through a special burnishing process or a plastic deformation, and the male component nests in the form of the female. The male component is partially D-Shaped to lock the two components together for easy removal. The thread of the tapered head of the female component has multi-leads and is synched with the multi-lead thread of the proximal head on the male components. The number of leads and the length of the thread are adaptable to the child's bone anatomy in such ay there is no intrusion of the bone joint. The male and female are locked together as an assembled nail and inserted simultaneously in the bone intramedullary canal. In case of revision or hardware removal surgeries, the assembly nail components are removed simultaneously together.

The invention discloses a multiple myeloma bone marrow image recognition system based on computer vision, relates to the technical field of cell recognition, solves the problem that traditional manual microscopic examination is large in limitation, and accurately recognizes burr change segments by conducting angle analysis on the edge contour of a feature area, so that the accuracy of recognition is improved. According to the comprehensive proportion of the burr change section on the overall edge contour, reliably judging whether the to-be-detected cell is a tumor cell or not, and calibrating and displaying the tumor cell in a gray image; according to the mode, the obvious difference of the tumor cells and normal cells in cell nucleus morphology is effectively utilized, rapid and accurate identification of the tumor cells is achieved, a visual and clear result is provided for clinical diagnosis, doctors are assisted to accurately judge multiple myeloma in time, the diagnosis efficiency and the treatment effect are improved, mean value processing and radial ratio analysis are conducted on contour area gray values, and the diagnosis accuracy is improved. The contour region associated with the cell nucleus can be accurately locked from the to-be-detected cell and is calibrated as the feature region, and a key cell nucleus feature basis is provided for tumor cell recognition.

The invention belongs to the technical field of biotechnology and animal models, and discloses a double-person-derived mouse model for simulating a tumor immune microenvironment and a construction method and application thereof. The method comprises the following steps: firstly, pretreating NSG immunodeficient mice by adopting low-dose whole-body irradiation in combination with double-antibody targeted bone marrow depletion, and transplanting CD34 + hematopoietic stem cells from the same human donor to complete human immune system reconstruction; separating tumor primary cells, tumor-related fibroblasts and tumor vascular endothelial cells of the same donor, performing three-dimensional co-culture to obtain homologous human tumor organs, and performing in-situ inoculation to immune reconstruction mice to obtain a target model. The core defects of MHC mismatching, low immune reconstruction efficiency, poor tumor immune microenvironment simulation degree, low clinical consistency and the like of an existing model are overcome, and the method can be used for tumor immune treatment drug screening, microenvironment mechanism research and personalized tumor treatment scheme verification.

Disclosed are methods of treating Ehlers-Danlos Syndrome in a subject by administering a therapeutic MSC secretome product made by a method comprising culturing bone marrow-derived MSCs under conditions that include oxygen tension below 5% and a culture media with a pH below 7.

The invention discloses a gastric cancer postoperative chemotherapy dynamic intervention method and system based on time point matching, and relates to the technical field of postoperative intervention. Health state data of wearable medical equipment of a gastric cancer postoperative patient is collected; preliminarily dividing the stomach cancer postoperative chemotherapy cycle into a plurality of key physiological time points based on the symptom inflection points, and respectively matching an intervention core problem and a corresponding stomach cancer postoperative chemotherapy intervention scheme for the plurality of key physiological time points; and the postoperative intervention platform analyzes the time period health state data collected by the wearable medical equipment at set time intervals, analyzes the health state data to automatically identify gastric cancer postoperative symptom inflection points, and dynamically matches key physiological time points based on symptom inflection point results. The stomach cancer postoperative treatment disruption rate is reduced, the crisis inflection point recognition accuracy is greatly improved, and the bone marrow suppression early diagnosis rate is greatly improved; the early diagnosis rate of postoperative wound infection of gastric cancer is improved, unnecessary intervention is reduced, and crisis event treatment is accelerated.

The invention relates to a recombinant complement protein and application thereof in preparation of anti-leukemia drugs, and belongs to the technical field of biological medicines. The invention discloses a method for treating acute leukemia by single drug, namely injecting recombinant C1QBP protein, which has a remarkable effect on treating acute leukemia, can be used for remarkably inhibiting proliferation of leukemia cells in marrow, spleen and peripheral blood of a hematopoietic system and also inhibiting infiltration of the leukemia cells in extramedullary organs (skin and the like), so that the whole-body tumor load is reduced, and the curative effect of treating acute leukemia is improved. The lifetime of leukemia mice is obviously prolonged. The recombinant C1QBP protein is combined with a clinical first-line chemotherapy regimen for use, has a synergistic effect, has a stronger effect of inhibiting leukemia cell proliferation compared with a single chemotherapy drug, can significantly prolong the lifetime of leukemia mice, and plays a role in reducing toxicity and enhancing efficacy. The invention proves that the complement C1Q binding protein has the effect of treating leukemia for the first time, provides a preparation method and a drug combination scheme, and has relatively high clinical transformation prospect and application value.

The invention provides a tibial plateau prosthesis and a preparation method, and belongs to the technical field of medical instruments. The tibial plateau prosthesis comprises a tibial plateau plate and an installation assembly, the tibial plateau plate comprises a plateau plate, a connecting sheet layer and a porous structure layer, the connecting sheet layer is arranged on the upper end face of the plateau plate, and the porous structure layer is arranged on the upper end face of the connecting sheet layer through a connecting structure; a solid surrounding edge is arranged on the outer edge of the porous structure layer, a mounting groove for mounting a tibial plateau liner is formed in the lower end face of the plateau plate, and the mounting assembly is arranged on the upper end face of the connecting sheet layer and is suitable for being implanted into a tibial marrow cavity. The invention provides a preparation method of a tibial plateau prosthesis. The preparation method comprises the operation steps of material preparation, 3D printing, semi-finished product disassembly, heat treatment, machining, polishing, cleaning, packaging and the like. The connection structure enhances the stability of connection between the porous structure layer and the connection sheet layer, and reduces the probability of falling of the porous structure.

The invention discloses a method for analyzing a mechanism of treating leucopenia by using a Shengbai oral liquid by using a network pharmacology mode, and the method is based on an HITS algorithm, and identifies key regulation and control nodes through hub and authority scores of nodes. An analysis result shows that PPBP, ALOXE3 and CCR1 are hub genes ranked in the first three, and PPBP not only has the highest hub score, but also shows the most significant expression change. In a bone marrow microenvironment, PPBP / CXCL7 may affect differentiation and maturation of hematopoietic stem cells and promote generation of specific types of leukocytes.

The invention relates to an application of extracellular beta-glucan derived from aureobasidium melanogenum in preparation of a training immunity inducer. By establishing a mouse bone marrow derived macrophages (BMDMs) and zebra fish juvenile fish training immune activation evaluation model, the immunocompetence of extracellular beta-glucan (eBG) derived from aureobasidium melanogenum is evaluated, and it is found that the extracellular beta-glucan has a more remarkable training immune activation effect compared with beta-glucan (cBG) derived from cell walls, and the immunocompetence of the extracellular beta-glucan (eBG) derived from aureobasidium melanogenum is improved. It is proved that the extracellular beta-glucan (eBG) from the melanin-producing aureobasidium is an excellent training immunity inducer and has a good application prospect in the fields of feed addition of bred animals and disease immune prevention and control.

The application relates to a wrist joint prosthesis and relates to the technical field of artificial joint prostheses. The wrist joint prosthesis comprises: a carpal bone assembly for being fixed with one side of a hand-shaped bone, which comprises a carpal bone pad, a carpal bone support block embedded in one side of the carpal bone pad, a carpal bone printing column and a screw connected to the other side of the carpal bone support block, and a support block adjusting device arranged on the embedded side of the carpal bone support block, one end of the support block adjusting device being located in the carpal bone pad, and the carpal bone pad being provided with a locking piece for locking the adjusted position of the support block adjusting device; and a radius bone assembly for being fixed with a radius bone marrow cavity, which comprises a radius bone support, the radius bone support being arranged on the side of the carpal bone pad away from the carpal bone support block. The wrist joint prosthesis has the advantages of shortening the waiting time for surgery.

The invention relates to a multiple myeloma model with extramedullary lesion as well as a construction method and application thereof. The multiple myeloma model is obtained by injecting bone marrow mononuclear cells (BMMC) of a multiple myeloma patient into an hIL-6NSG mouse. The invention provides a new model of multiple myeloma with extramedullary lesion, which not only is beneficial to research on gene pathway and mechanism of the multiple myeloma, but also lays a foundation for research on tumor recurrence, preclinical drug screening, effect evaluation and the like, and has great significance in promoting disease healing.

The invention belongs to the field of intelligent equipment, and particularly relates to a system for automatically distinguishing leukemia types based on flow cytometry. The invention aims to solve the problem of low leukemia identification accuracy in the prior art. The invention provides a system for automatically distinguishing leukemia types based on flow cytometry. The system comprises a data acquisition module, a feature analysis module and a prediction and diagnosis module, the data acquisition module is used for processing bone marrow puncture fluid of a to-be-detected person to obtain a cell event data set of the to-be-detected person; the feature analysis module is used for processing a cell event data set of a to-be-detected person to obtain extraction features; the prediction and diagnosis module is used for processing extracted feature prediction to obtain a diagnosis conclusion; according to the system, through a standardized data preprocessing process, the analysis duration can be compressed to be within 20 minutes, the diagnosis consistency is improved to 98% or above, and the detection sensitivity reaches the 0.001% level.

The invention discloses a thighbone inverted-hitting intramedullary nail entry point guide plate and a preparation method thereof, and aims to improve the precision and efficiency of a thighbone intramedullary nail operation. The guide plate comprises an attaching plate and a guide column, the attaching plate is provided with an attaching face precisely matched with a femoral intercondylar fossa and an inner side condylar area, it is ensured that the guide plate can be stably attached to the distal end of the femur, and stable support is provided for an operation. One end of the guide column is connected with the pasting plate, the other end of the guide column extends towards the proximal femur, a guide hole is formed in the guide column, the central axis of the guide hole is strictly collinear with a preset needle inserting point at the distal femur, and reliable guidance is provided for accurate insertion of an intramedullary nail. The guide plate can be prepared before an operation, assists a doctor in quickly positioning a needle inserting point during the operation, effectively shortens the operation time, improves the operation efficiency, reduces the operation risk, and provides an efficient and accurate auxiliary tool for the femoral intramedullary nail operation.

The invention relates to the technical field of veterinary chemical drugs, and provides an application of a selenium-rich composition in a drug for preventing bone marrow suppression after chemotherapy of dogs, and the selenium-rich composition comprises nano-selenium-beta-glucan chelate and L-selenium-methyl selenocysteine, the mass ratio of the nano-selenium-beta-glucan chelate to the L-selenium-methyl selenocysteine is 1: (0.3-0.6), and the mass ratio of the nano-selenium-beta-glucan chelate to the L-selenium-methyl The molar ratio of the astragaloside IV to the cobamamide is (5 to 8): 1. Through the synergistic effect of the nano-selenium-beta-glucan chelate and the L-selenium-methyl selenocysteine and in combination with the loading technology of the silicon dioxide mesoporous carrier, the absorption rate of selenium is greatly improved.

The invention relates to the technical field of medullo-puncture needles, and discloses a medullo-puncture needle convenient to fix, which comprises a base and a needle body, a hollow turntable is rotatably mounted on the surface of the base, an adjusting cylinder is rotatably mounted in the turntable up and down, and the needle body is detachably mounted in the adjusting cylinder; a threaded rod is installed in the adjusting cylinder in a threaded connection mode, and the threaded rod is used for driving the needle body to move in the axial direction. According to the convenient-to-fix medullo-puncture needle, a rotatable adjusting cylinder is arranged on the surface of the base, a fixing ring capable of driving a needle body to enter the medullo-puncture needle, a toothed bar and a threaded rod are arranged in the adjusting cylinder, and meanwhile, an upper driving gear and a lower driving gear in the speed change assembly are matched with an upper toothed ring and a lower toothed ring, so that the puncture angle of the needle body can be adjusted quickly and slowly; the needle body puncture angle can be accurately adjusted, fast adjustment and slow adjustment of the needle body puncture speed can also be achieved, accurate adjustment of the needle body puncture depth position is achieved, and therefore the needle body puncture effect is improved.

The invention is suitable for the field of meat processing and production, and provides an intelligent recognition method and system for the three-dimensional contour of boned meat based on image recognition, and the method comprises the steps: carrying out the multi-modal image collection of frozen boned meat, and capturing a surface crack pattern; analyzing the crack pattern to construct a topological network model; reconstructing a bone-meat three-dimensional holographic model based on the topological network model, and marking joint, marrow cavity and bone mineral density abnormal areas; identifying potential skeleton weak points; generating a double-path cutting guide line; cooperative operation and visual presentation of the guide lines are controlled through dynamic compensation. Precise recognition and intelligent cutting guide of the internal skeleton structure of the boned meat are achieved, the segmentation precision and efficiency are improved, and the method is suitable for the field of meat processing and the like.

The invention provides application of apoptotic cell pretreated macrophages in preparation of an osteoarthritis treatment preparation, a cell preparation and a preparation method, and relates to the technical field of biomedicine.In the embodiment of the invention, based on a mouse development model and an osteoarthritis (OA) model, it is found that in the area away from the cartilage surface, the apoptotic cell pretreated macrophages are subjected to apoptotic cell pretreatment, and the apoptotic cell pretreated macrophages are subjected to apoptotic cell pretreatment in the area away from the cartilage surface; in the macrophages, the content of the interment-related LAMP1 protein is obviously higher than that of the macrophages close to the cartilage surface area, and the expression results of TGF-beta 1 and LRG1 are respectively opposite and consistent with those of the LAMP1. The invention also finds that after the chemotactic effect of apoptotic cells on macrophages is weakened, the cellular burial effect is limited, and the cartilage matrix development disorder is caused. Based on development process inspiration, the invention provides a treatment strategy: bone marrow-derived macrophages pretreated by apoptotic cells are injected into articular cavities, and a good cartilage regeneration treatment effect can be achieved in a mouse arthritis model. In addition, the bone marrow-derived macrophages pretreated by apoptotic cells can still express TGF-beta1 after being injected into the body for 3 days, so that the effect of continuously promoting cartilage regeneration is achieved.

The invention discloses a radix astragali, rhizoma polygonati and ligustrum lucidum ait traditional Chinese medicine preparation for treating myelosuppression as well as a preparation method and a detection method thereof. The preparation is prepared from 50-150 parts of radix astragali, 50-150 parts of wine-processed rhizoma polygonati, 25-75 parts of wine-processed ligustrum lucidum ait, 50-150 parts of blanched rhizoma drynariae, 50-150 parts of radix trichosanthis and 25-75 parts of herba epimedii. The optimal extraction process and refining process are screened out based on effective components and pharmacological activity through a large number of tests, and the optimal preparation process of the Shuanghuangliangwhitening oral liquid and other traditional Chinese medicine preparations is obtained through stability investigation and verification. According to the invention, a thin layer chromatography is adopted, the four medicinal materials of astragalus membranaceus, glossy privet fruit, rhizoma drynariae and herba epimedii are respectively subjected to thin layer chromatography research, and methodology research shows that the method is stable and feasible. The invention establishes a content determination method for astragaloside, calycosin-7-glucoside, specnuezhenide and icariin, establishes a quality standard, and has important significance for ensuring the clinical curative effect and safety of astragaloside, calycosin-7-glucoside, specnuezhenide and icariin.

The invention relates to an antibacterial peptide CnCATH as well as a coding gene and application thereof, and belongs to the technical field of biomedicine. The novel antibacterial peptide is extracted from sika deer bone marrow, and the amino acid sequence is as shown in SEQ ID NO. 2. The antibacterial peptide shows strong antibacterial activity on various microorganisms including gram-negative bacteria, gram-positive bacteria and fungi, the sterilization speed of the antibacterial peptide is obviously improved compared with that of traditional antibiotics, the bacterial strain is not prone to drug resistance, endotoxin can be further neutralized, and the concentration of the endotoxin can be further reduced. The novel sika deer-derived antibacterial peptide provided by the invention has the advantages of small molecular weight, simple artificial synthesis, low drug resistance tendency and high broad-spectrum bactericidal activity, and provides a new strategy for microbial anti-infection treatment and prevention and treatment of diseases caused by endotoxin.

The invention belongs to the technical field of medical image processing and deep learning, and discloses a bone marrow pathology image analysis system based on multi-view clustering, which comprises the following steps: firstly, acquiring a bone marrow full slice image and a corresponding pathology report, and respectively extracting multi-scale image features and text semantic features; constructing a pyramid hypergraph fusing spatial topology and semantic association, and aggregating multi-scale instance information through a graph attention network to generate image-level global features; then adaptively learning a cross-modal consensus prototype, calculating semantic similarity to obtain a soft distribution probability, and solving an optimal pseudo tag; and finally, realizing bimodal feature alignment by means of symmetric cross entropy knowledge distillation and entropy regularization, and outputting a classification result through clustering distribution.

The invention discloses a spiral reinforced composite structure artificial ligament with a bundle characteristic and a preparation method thereof, and belongs to the technical field of biomedical materials. The artificial ligament is of a composite structure, is composed of a plurality of units and has a bundle-shaped characteristic. When the artificial ligament is stretched in the axial direction, the negative Poisson's ratio effect is achieved in the direction perpendicular to the axial direction. The structure of each unit is a spiral reinforced knitted structure and is prepared and formed by adopting a textile technology. The artificial ligament is formed by weaving a medical-grade fiber material or a surface-modified fiber material with biocompatibility. The physical characteristics such as the size, the surface roughness, the surface mesh size, the tensile mechanical behavior and the rigidity of the artificial ligament can be regulated and controlled through the design of a textile weave structure and process parameters. The physical performance of the artificial ligament can be regulated and controlled by changing the preparation process, tissue growth is better promoted so as to be better combined with a marrow tract interface, and the nonlinear stretching mechanical behavior of a natural ligament under the stress working condition is simulated.

The utility model relates to the technical field of medical instruments, and discloses a marrow cavity transfusion and blood transfusion puncture outfit which comprises a puncture system, the puncture system comprises a hollow puncture needle and a positioning seat, one end of the puncture needle is arranged on the positioning seat, and the other end of the puncture needle is of a tip structure; the plugging system comprises a sealing rod and a locking seat, one end of the sealing rod is arranged on the locking seat, the other end of the sealing rod is inserted into the puncture needle, and the locking seat is detachably connected with the positioning seat; the power system comprises a gun body, a driving part and a transmission seat, the driving part is arranged in the gun body, the transmission seat is arranged on the gun body, the transmission seat is detachably connected with the positioning seat, and the driving part can drive the positioning seat to rotate through the transmission seat. And the puncture accuracy is improved.

Systems and methods for reaming an intramedullary canal of a long bone comprise a trial stem configured to extend into the long bone along an insertion axis and a guide device comprising an adapter configured to couple to the trial stem and a reaming guidepost extending from the adapter along a guide axis, wherein the guide axis and the insertion axis are non-aligned. A method of reaming an intramedullary canal of a long bone to form a complex shaped socket can comprise inserting a stem into the intramedullary canal along an insertion axis, connecting a guide device to the stem, the guide device comprising a guidepost extending along a guide axis and guiding a cannulated reamer along the guidepost to remove bone from the intramedullary canal to form the complex shaped socket, wherein the guide axis and the insertion axis are non-aligned.

The invention discloses an antibody combination, a kit and a method for detecting each development subgroup of bone marrow myeloid cells by flow cytometry. And the antibodies comprise an anti-CD45 antibody, an anti-HLA-DR antibody, an anti-CD13 antibody, an anti-CD11b antibody, an anti-CD34 antibody, an anti-CD64 antibody, an anti-CD45RA antibody, an anti-CD123 antibody, an antiLin antibody and an anti-CD38 antibody. According to the invention, the myeloid cells in the bone marrow microenvironment before and after hematologic tumor treatment can be comprehensively, specifically and conveniently dynamically monitored.

The invention discloses a bionic bone marrow microenvironment assembly for hematopoietic stem cell in-vitro amplification and a preparation method. The bone marrow microenvironment assembly comprises a three-dimensional scaffold and osteoblasts (OBs), wherein the three-dimensional scaffold comprises chitosan (CS) and sodium alginate (Alg) which are prepared into mixed gel, and the mixed gel is freeze-dried to prepare the three-dimensional scaffold; wherein the osteoblasts (OBs) are obtained by culturing and induced differentiation of umbilical cord mesenchymal stem cells (MSCs) inoculated on a three-dimensional scaffold, and the osteoblasts (OBs) as stromal cells and the three-dimensional scaffold jointly form the hematopoietic stem cell in-vitro amplification bionic bone marrow microenvironment assembly; in the hematopoietic stem cell in-vitro amplification bionic bone marrow microenvironment assembly, the preparation of the three-dimensional scaffold comprises the processes of freezing, remelting, freezing, crosslinking, freeze-drying and the like, so that a three-dimensional space form with wrinkles on the pore surface and pore size distribution between 70 microns and 400 microns is generated in the three-dimensional scaffold, and the three-dimensional space form is closer to a real stem cell niche; therefore, the practical application effect of the bionic bone marrow microenvironment assembly for in-vitro amplification of the hematopoietic stem cells is further improved.

The present disclosure provides efficient in vitro methods for producing megakaryocytes and / or platelets from human induced pluripotent stem cells (iPSCs). In various embodiments, the cells and platelets produced in accordance with the present disclosure are functional and / or more closely similar to corresponding lineages isolated from peripheral blood, bone marrow, or other tissue. The present invention provides, in some aspects, isolated cells / platelets and compositions produced by the methods disclosed herein, as well as methods for use in therapy.