418 results about "Bone marrow" patented technology

The invention discloses an intelligent gradient extraction process and method for nutritional ingredients in yak bone marrow, and belongs to the technical field of bioactive substance extraction. The extraction method comprises the following four steps: gradient pressure supercritical degreasing, double-enzyme synergistic gradient enzymolysis, acid concentration gradient demineralization and temperature gradient gelatin extraction, and through the process coupling design of gradient pressure degreasing, buffer enzymolysis, graded acidolysis and membrane separation of gelatin, the high yield of the product in the previous stage is ensured; active structures of follow-up components are reserved to the greatest extent. Meanwhile, a sensing-decision-execution closed-loop system is constructed, real-time data sources such as NIR moisture sensing, online OPA detection and XRD in-situ analysis are integrated, technological parameters are dynamically adjusted through a multi-objective optimization algorithm, and global optimization of the yield, the purity and the energy consumption is achieved.

The invention belongs to the technical field of biotechnology and animal models, and discloses a double-person-derived mouse model for simulating a tumor immune microenvironment and a construction method and application thereof. The method comprises the following steps: firstly, pretreating NSG immunodeficient mice by adopting low-dose whole-body irradiation in combination with double-antibody targeted bone marrow depletion, and transplanting CD34 + hematopoietic stem cells from the same human donor to complete human immune system reconstruction; separating tumor primary cells, tumor-related fibroblasts and tumor vascular endothelial cells of the same donor, performing three-dimensional co-culture to obtain homologous human tumor organs, and performing in-situ inoculation to immune reconstruction mice to obtain a target model. The core defects of MHC mismatching, low immune reconstruction efficiency, poor tumor immune microenvironment simulation degree, low clinical consistency and the like of an existing model are overcome, and the method can be used for tumor immune treatment drug screening, microenvironment mechanism research and personalized tumor treatment scheme verification.

The invention provides a tibial plateau prosthesis and a preparation method, and belongs to the technical field of medical instruments. The tibial plateau prosthesis comprises a tibial plateau plate and an installation assembly, the tibial plateau plate comprises a plateau plate, a connecting sheet layer and a porous structure layer, the connecting sheet layer is arranged on the upper end face of the plateau plate, and the porous structure layer is arranged on the upper end face of the connecting sheet layer through a connecting structure; a solid surrounding edge is arranged on the outer edge of the porous structure layer, a mounting groove for mounting a tibial plateau liner is formed in the lower end face of the plateau plate, and the mounting assembly is arranged on the upper end face of the connecting sheet layer and is suitable for being implanted into a tibial marrow cavity. The invention provides a preparation method of a tibial plateau prosthesis. The preparation method comprises the operation steps of material preparation, 3D printing, semi-finished product disassembly, heat treatment, machining, polishing, cleaning, packaging and the like. The connection structure enhances the stability of connection between the porous structure layer and the connection sheet layer, and reduces the probability of falling of the porous structure.

The invention is suitable for the field of meat processing and production, and provides an intelligent recognition method and system for the three-dimensional contour of boned meat based on image recognition, and the method comprises the steps: carrying out the multi-modal image collection of frozen boned meat, and capturing a surface crack pattern; analyzing the crack pattern to construct a topological network model; reconstructing a bone-meat three-dimensional holographic model based on the topological network model, and marking joint, marrow cavity and bone mineral density abnormal areas; identifying potential skeleton weak points; generating a double-path cutting guide line; cooperative operation and visual presentation of the guide lines are controlled through dynamic compensation. Precise recognition and intelligent cutting guide of the internal skeleton structure of the boned meat are achieved, the segmentation precision and efficiency are improved, and the method is suitable for the field of meat processing and the like.

The invention discloses a radix astragali, rhizoma polygonati and ligustrum lucidum ait traditional Chinese medicine preparation for treating myelosuppression as well as a preparation method and a detection method thereof. The preparation is prepared from 50-150 parts of radix astragali, 50-150 parts of wine-processed rhizoma polygonati, 25-75 parts of wine-processed ligustrum lucidum ait, 50-150 parts of blanched rhizoma drynariae, 50-150 parts of radix trichosanthis and 25-75 parts of herba epimedii. The optimal extraction process and refining process are screened out based on effective components and pharmacological activity through a large number of tests, and the optimal preparation process of the Shuanghuangliangwhitening oral liquid and other traditional Chinese medicine preparations is obtained through stability investigation and verification. According to the invention, a thin layer chromatography is adopted, the four medicinal materials of astragalus membranaceus, glossy privet fruit, rhizoma drynariae and herba epimedii are respectively subjected to thin layer chromatography research, and methodology research shows that the method is stable and feasible. The invention establishes a content determination method for astragaloside, calycosin-7-glucoside, specnuezhenide and icariin, establishes a quality standard, and has important significance for ensuring the clinical curative effect and safety of astragaloside, calycosin-7-glucoside, specnuezhenide and icariin.

The invention belongs to the technical field of medical image processing and deep learning, and discloses a bone marrow pathology image analysis system based on multi-view clustering, which comprises the following steps: firstly, acquiring a bone marrow full slice image and a corresponding pathology report, and respectively extracting multi-scale image features and text semantic features; constructing a pyramid hypergraph fusing spatial topology and semantic association, and aggregating multi-scale instance information through a graph attention network to generate image-level global features; then adaptively learning a cross-modal consensus prototype, calculating semantic similarity to obtain a soft distribution probability, and solving an optimal pseudo tag; and finally, realizing bimodal feature alignment by means of symmetric cross entropy knowledge distillation and entropy regularization, and outputting a classification result through clustering distribution.

The invention discloses a spiral reinforced composite structure artificial ligament with a bundle characteristic and a preparation method thereof, and belongs to the technical field of biomedical materials. The artificial ligament is of a composite structure, is composed of a plurality of units and has a bundle-shaped characteristic. When the artificial ligament is stretched in the axial direction, the negative Poisson's ratio effect is achieved in the direction perpendicular to the axial direction. The structure of each unit is a spiral reinforced knitted structure and is prepared and formed by adopting a textile technology. The artificial ligament is formed by weaving a medical-grade fiber material or a surface-modified fiber material with biocompatibility. The physical characteristics such as the size, the surface roughness, the surface mesh size, the tensile mechanical behavior and the rigidity of the artificial ligament can be regulated and controlled through the design of a textile weave structure and process parameters. The physical performance of the artificial ligament can be regulated and controlled by changing the preparation process, tissue growth is better promoted so as to be better combined with a marrow tract interface, and the nonlinear stretching mechanical behavior of a natural ligament under the stress working condition is simulated.

The invention discloses a bionic bone marrow microenvironment assembly for hematopoietic stem cell in-vitro amplification and a preparation method. The bone marrow microenvironment assembly comprises a three-dimensional scaffold and osteoblasts (OBs), wherein the three-dimensional scaffold comprises chitosan (CS) and sodium alginate (Alg) which are prepared into mixed gel, and the mixed gel is freeze-dried to prepare the three-dimensional scaffold; wherein the osteoblasts (OBs) are obtained by culturing and induced differentiation of umbilical cord mesenchymal stem cells (MSCs) inoculated on a three-dimensional scaffold, and the osteoblasts (OBs) as stromal cells and the three-dimensional scaffold jointly form the hematopoietic stem cell in-vitro amplification bionic bone marrow microenvironment assembly; in the hematopoietic stem cell in-vitro amplification bionic bone marrow microenvironment assembly, the preparation of the three-dimensional scaffold comprises the processes of freezing, remelting, freezing, crosslinking, freeze-drying and the like, so that a three-dimensional space form with wrinkles on the pore surface and pore size distribution between 70 microns and 400 microns is generated in the three-dimensional scaffold, and the three-dimensional space form is closer to a real stem cell niche; therefore, the practical application effect of the bionic bone marrow microenvironment assembly for in-vitro amplification of the hematopoietic stem cells is further improved.

This disclosure provides: (i) a method of using an anticeramide antibody or an antigen-binding fragment thereof to improve hematopoietic stem cell transplantation for the treatment of various non-malignant and malignant diseases; and (ii) a composition for such treatment.

An implant assembly for a long bone is adapted to support an exo-prosthesis. The assembly includes a stem, a subdermal component and a percutaneous post. The stem has a proximal end that is adapted to be received in a surgically prepared medullary canal of the long bone, and a distal end having a surface that is adapted to promote bone ingrowth. The subdermal component includes a proximal portion that is adapted for attachment to the distal end of the stem, and a fixation surface that is adapted to promote soft tissue fixation. The percutaneous post has a proximal end that is adapted to be attached to the subdermal component.

The invention relates to the technical field of biomedical engineering and drug delivery, and particularly discloses a novel targeted drug delivery system based on rose fruit extracellular vesicles, which comprises the following steps: preparing rose fruit source extracellular vesicles; preparing a medicine carrying vesicle; preparing targeted modified drug-loaded vesicles; according to the application disclosed by the invention, a stem related pathway is specifically down-regulated through the components of the rose fruits, and meanwhile, conventional tumor cells are killed by using adriamycin, so that a complementary treatment mechanism is formed, and the tumor recurrence risk is fundamentally reduced. The phenylboronic acid group of DSPE-PEG-PBA is used for specifically recognizing sialic acid over-expressed on the surface of a tumor cell, so that the enrichment of the medicine in tumor tissues is remarkably improved, and meanwhile, the distribution of normal tissues is reduced. By means of the natural biocompatibility and low immunogenicity of the RHNVs and in combination with the long circulation characteristic of a PEG chain, the delivery efficiency is guaranteed, the systemic toxicity is reduced to the maximum extent, and particularly the cardiotoxicity and myelosuppression of adriamycin are relieved.

The invention discloses a mesenchymal stem cell with a PEG (polyethylene glycol) coating on the surface as well as a preparation method and application of the mesenchymal stem cell. According to the invention, polyethylene glycol, epigallocatechin gallate and magnesium ions are adopted to form a coating for surface modification of mesenchymal stem cells. The lung closure of the MSC is reduced, the in-vivo survival rate of the MSC is increased, the bone marrow targeting is damaged, and the capability of resisting oxidative stress severe microenvironment is improved.

The invention discloses a pavement defect identification method based on image processing, and relates to the technical field of image processing. Constructing a pavement defect recognition model, wherein the pavement defect recognition model comprises a backbone module, a neck module and a head module which are connected in sequence; the backbone module comprises a convolution module and an STAttention attention module; the convolution module comprises a DWConv convolution layer, two parallel PConv layers and a fusion module which are connected in sequence; obtaining a pavement defect image data set, and training the pavement defect recognition model based on the pavement defect image data set to obtain a trained pavement defect recognition model; and inputting the to-be-recognized pavement image into the pavement defect recognition model, and outputting the category probability and the coordinate frame corresponding to the pavement defect through the pavement defect recognition model. According to the invention, the recognition accuracy of pavement defects can be improved.

The invention relates to the technical field of biology, in particular to application of a GGPS1 activator, a pharmaceutical composition, a kit and a method. The invention relates to bone metabolism regulation and bone regeneration. It is found that improvement of GGPS1 expression / activity can activate TGF-beta / Smad2 / 3 by down-regulating LTBP1, so that human bone marrow mesenchymal stem cells are converted from adipogenesis to osteogenesis, and bone formation and bone defect repair are promoted. The invention provides a drug containing a GGPS1 activator and a controlled release composition for implantation, which are used for osteoporosis, bone mass reduction, delayed fracture healing and the like. The invention also provides an in-vitro screening and evaluation method and a detection kit, and the indexes such as p-Smad2 / 3, alizarin red, oil red O and the like are used for judgment. The invention realizes upstream heavy balance and has the advantages of bone promotion and fat suppression.

The application relates to an image-based marrow component segmentation method and system, comprising the following steps: acquiring an original marrow biopsy digital image and preprocessing, obtaining a hematopoietic cell tissue and bone trabecula Mask mask image based on the preprocessed image; based on the original marrow biopsy digital image, sequentially performing color channel conversion, morphological open operation, isolated point elimination and hole filling to obtain a bone trabecula Mask mask image; based on the preprocessed image, obtaining a fat cell tissue Mask mask image according to the morphological characteristics of fat cells; and according to the obtained bone trabecula Mask mask image, the bone trabecula and hematopoietic cell tissue Mask mask image and the fat cell tissue Mask mask image, obtaining the area proportion of the bone trabecula, the hematopoietic cell tissue and the fat cell tissue in the original marrow biopsy digital image in each image.

The invention relates to the field of gene mutation detection, and particularly discloses a POCT (point-of-care testing) detection method for V617F mutation of a human JAK2 gene. According to the method, an integrated detection tube is divided into a cell lysis-nucleic acid binding magnetic bead area, a nucleic acid cleaning area and a nucleic acid elution-amplification area from top to bottom in sequence through a plurality of hydrophobic layers, the cell lysis-nucleic acid binding magnetic bead area is pre-filled with a lysis solution, the nucleic acid cleaning area is pre-filled with a nucleic acid cleaning solution, and the nucleic acid elution-amplification area is pre-filled with a nucleic acid elution solution. A nucleic acid elution-amplification system is pre-installed in the nucleic acid elution-amplification area, in the detection process, only a sample needs to be added into the cell lysis-nucleic acid binding magnetic bead area, a corresponding instrument is inserted, and operation is conducted, so that nucleic acid extraction, amplification and result reporting can be completed, and additional manual processing steps are not needed. The method has the advantages of simplicity and convenience in operation, short time consumption, high sensitivity, strong specificity, low pollution risk and the like, can be directly used for whole blood sample detection, meets the rapid diagnosis requirements of clinical outpatient service, and is particularly suitable for auxiliary diagnosis of BCR-ABL negative myeloproliferative tumors.

The invention belongs to the technical field of hotpot condiments, and particularly relates to a bovine bone marrow oil mellow and hot hotpot condiment and a preparation method thereof. Comprising the following raw materials in parts by weight: 205 to 298 parts of edible beef tallow, 9.5 to 14 parts of edible bovine bone marrow oil, 15 to 25 parts of dried chili, 18 to 26 parts of chafing dish bean halves, 4 to 6 parts of fermented soya beans, 13 to 20 parts of monosodium glutamate, 10 to 16 parts of edible salt, 7 to 12 parts of fresh ginger, 9 to 14 parts of garlic, 7 to 11 parts of shallots, 10 to 16 parts of spices, 4.5 to 7 parts of yeast extract, 3 to 4.5 parts of white granulated sugar, 0.5 to 0.97 part of disodium 5 '-ribonucleotide, 15.8 to 23.8 parts of edible flavors and fragrances and 0.5 to 0.9 part of capsanthin. The edible bovine bone marrow oil in the mellow and hot hotpot condiment product containing the bovine bone marrow oil has rich and deep animal fat fragrance, the fragrance is mellower than beef tallow, the condiment is good in mellowness, and the flavor and taste are improved.

A system and method of isolating extracellular vesicles. The method includes loading one or more of blood or bone marrow into an input port of a concentration system and centrifuging one or more of the blood or bone marrow to separate one or more of red blood cells, platelet poor plasma, or platelet rich plasma / bone marrow concentrate fractions via a centrifuge device. The method further includes pumping one or more of bone marrow / platelet rich plasma fractions and platelet poor plasma fractions into a receptacle of the concentration system and adding a concentrated aqueous two-phase solution to one or more of the bone marrow concentrate / platelet rich plasma fractions and platelet poor plasma fractions. The method also includes drawing the concentrated aqueous two-phase solution and one or more of the bone marrow concentrate / platelet rich plasma fractions or platelet poor plasma fractions back into the centrifuge device to isolate one or more of extracellular vesicles and platelet rich plasma / bone marrow concentrate fractions.

The invention provides an anti-anemia traditional Chinese medicine composition as well as a preparation method and application thereof. The anti-anemia traditional Chinese medicine composition is prepared from lithocarpus litseifolius, fructus gardeniae and rhizoma gastrodiae. The main active component dihydrochalcone (such as phlorizin, trilobatin, phloretin and the like) of lithocarpus litseifolius can improve iron metabolism by regulating a hepcidin-iron transporter axis; gardenoside in gardenia can target the liver to optimize iron distribution, and gastrodia elata polysaccharide can regulate immunity and improve the marrow microenvironment; the three forms a full-chain regulation and control network of intestinal tract iron absorption, liver iron utilization and bone marrow hematopoiesis microenvironment together, and the anti-anemia effect is enhanced in multiple dimensions.

A polyclonal antibody composition specifically binds the pTonB epitope (SEQ ID NO: 11) from Acinetobacter baumannii and is elicited by immunization with a multi-epitope antigen comprising thioredoxin leader, rigid linker, and kernels including SEQ ID NOs: 6, 7, 11, 12, and 8. These antibodies enhance opsonophagocytic killing of A. baumannii Ci79 via classical complement activation and Fey receptor-mediated phagocytosis by bone marrow-derived macrophages. Absorption of pTonB-specific antibodies reduces killing by >70%, confirming epitope dominance. Passive transfer of the composition protects >60% of mice in a lethal intranasal challenge model. Pharmaceutical compositions, treatment methods (alone or with antibiotics like colistin), prophylactic uses, diagnostic kits, and polyclonal compositions are disclosed for combating multidrug-resistant A. baumannii infections.

The invention discloses a medicine formula for preventing cerebral infarction and promoting rehabilitation and a preparation method thereof, and belongs to the technical field of preparation of medicines for preventing cerebral infarction, the medicine formula comprises the following raw materials by weight: 10-15 parts of flatstem milkvetch seed, 10-15 parts of dodder, 10-15 parts of tortoise plastron, 10-12 parts of red peony root, 6-9 parts of safflower, and 15-30 parts of red sage root. According to the invention, the flatstem milkvetch seed, the semen cuscutae and the tortoise plastron are used for nourishing liver and kidney, replenishing essence and benefiting marrow, so that the traditional Chinese medicine theory that kidney dominates bone marrow generation and brain is marrow sea is met, and brain function decline is improved from the source; meanwhile, the radix salviae miltiorrhizae, the radix paeoniae rubra and the flos carthami have the effects of promoting blood circulation to remove blood stasis, activating meridians to stop pain, effectively improving brain microcirculation, inhibiting platelet aggregation and reducing blood viscosity, and supplement each other to achieve the effects of strengthening the body resistance to eliminate pathogenic factors and treating both symptoms and root causes, so that the traditional Chinese medicine composition can be used for preventing cerebral infarction of high-risk groups and is also suitable for functional recovery in the rehabilitation period after cerebral infarction.

A method of treating or preventing central nervous system cancer in a subject in need thereof is described. The method includes administering to the subject a therapeutically effective amount of myeloid cells modified to express interleukin-2. A population of genetically engineered myeloid cells (GEMys) comprising bone marrow derived myeloid cells that have been genetically modified to express interleukin-2 is also described.

The Abstract of this product is to provide a safe hard long-lasting chew for dogs. There is no choking hazard as it is liquid that's dried. Any dog of any size or age can safely consume the product.

The invention discloses a composition for improving adverse effects of chemotherapy as well as a preparation method, application and a product of the composition, and particularly relates to the technical field of medicines. The composition comprises the following raw materials: tortoise, soft-shelled turtle, antler, sea cucumber, fructus amomi, pericarpium citri reticulatae and hawthorn. Alkaline protease and flavourzyme are adopted for enzymolysis, and the obtained composition can remarkably reduce the tumor volume of tumor-bearing animals, increase the bone marrow DNA content of chemotherapy animals and remarkably increase the number of white blood cells and the number of red blood cells in peripheral blood of the chemotherapy animals. The composition disclosed by the invention is used for preparing related products for improving the adverse effects of chemotherapy, and the prepared products are easy to absorb and have a remarkable improvement effect on the adverse effects of chemotherapy.

One embodiment of the present invention provides a method of producing a fibrous non-demineralized bone graft material, the method comprising: a step of removing soft tissue, blood, and bone marrow from bone; a dehydration step of immersing, in an aqueous ethanol solution, the bone from which the soft tissue, blood, and bone marrow have been removed, and stirring same; a primary defatting step of immersing and stirring the dehydrated bone in diethyl ether; a step of crushing the bone subjected to the primary defatting into fibrously processed bone; a step of screening the fibrously processed bone; a hydration step of immersing and stirring the screened fibrously processed bone in ethanol; a secondary defatting step of immersing and stirring the hydrated fibrously processed bone in hydrogen peroxide; a washing step of washing the fibrously processed bone subjected to the secondary defatting; and a freeze-drying step.

The invention provides a pharmaceutical composition for treating non-small cell lung cancer and a use method, and belongs to the technical field of tumor immunotherapy. The invention formulates a non-small cell lung cancer weak chemotherapy and anti-vascular immunity triple treatment scheme based on the combination of a low-dose chemotherapy drug pemetrexed, an immune checkpoint inhibitor and an anti-angiogenesis drug. According to the scheme, low-dose pemetrexed is combined with the anti-angiogenesis medicine and the immune checkpoint inhibitor for use, the optimal synergistic effect can be achieved, meanwhile, the renal toxicity and the myelosuppression risk are remarkably reduced, and the pemetrexed medicine composition is expected to become one of the optimal choices for driving gene negative and PD-L1 positive expression non-squamous NSCLC patients, and has a wide application prospect. The device is suitable for old people or patients with poor physical states; especially for patients with negative driver genes and positive PD-L1 expression, the traditional treatment blank is filled, and clinical data shows that survival benefits are remarkable.

The invention belongs to the technical field of pharmacy, and particularly relates to application of urolithin A in treating multiple organ injuries caused by sepsis. Experiments prove that the urolithin A can significantly reduce the apoptosis rate of bone marrow-derived macrophages, and can effectively relieve multi-organ injury caused by sepsis by regulating three key paths including oxidative stress, cell apoptosis and necrotic apoptosis. The application provides solid experimental basis and theoretical support for development of urolithin A as a sepsis treatment drug, and has important conversion value and clinical application prospect in the critical disease medical field.

A method for treating cartilage defects including providing a placental membrane preparation that comprises ground or minced placental membranes and optionally, a ground or minced cartilage and / or biocompatible glue, and introducing the preparation to a cartilage defect within a skeletal joint. The cartilage defect may comprise a hyaline cartilage defect, such as a chondral defect, or meniscal defect. The treatment may be provided in combination with other treatments such as marrow stimulation treatments and surgical repair treatments using sutures or other fixation techniques. The preparation promotes the regeneration of cartilage within the skeletal joint.

The invention provides a biomarker for predicting chemosensitivity of acute myelogenous leukemia and application of the biomarker, and belongs to the technical field of biological medicine. The leptin and fumaric acid provided by the invention can be used as biomarkers for predicting AML chemotherapy resistance. Wherein the leptin influences the chemotherapy sensitivity by regulating metabolic pathways such as mitochondrial OXPHOS and mitochondrial active oxygen mtROS; meanwhile, the invention also discloses a new mechanism that fumaric acid promotes BMSCs to transfer mitochondria to AML cells so as to enhance the OXPHOS level and induce Ara-C drug resistance. The levels of the two are obviously related to the chemotherapy reaction and prognosis stratification of the patient, so that early recognition of the patient with chemotherapy resistance or poor prognosis is facilitated. Leptin and fumaric acid can be detected in bone marrow plasma, the feasibility is high, and clinical transformation is facilitated. By detecting the level of leptin or fumaric acid in bone marrow of a patient, individualized treatment can be achieved, and the survival rate of the patient is increased.

The invention discloses a preparation method of collagen micromolecule active peptide and bioactive peptide for inhibiting cancer cell proliferation and improving intracellular environment, and belongs to the technical field of bioactive peptide preparation and biological functional raw materials. According to the method, bone collagen peptide, cartilage peptide (hydrolyzed II-type collagen peptide), collagen peptide (German Jilida), non-denatured II-type collagen peptide and yak bone marrow peptide are taken as core raw materials, and optional ingredients such as bovine bone marrow peptide powder, shark cartilage peptide and Belgian imported inulin can be compounded as required; through a step-by-step preparation process comprising the steps of graded purification pretreatment of raw materials, precise proportioning and mixing, low-temperature activation and synergism and stable micro-capsule embedding, structure protection and efficacy synergy of each collagen small-molecule active peptide and bioactive components are realized; the prepared bioactive peptide product can significantly inhibit cancer cell proliferation, regulate intracellular oxidation-reduction balance and improve intracellular microenvironment, the preparation process is mild and free of high-temperature damage, the raw material biological activity retention rate is high, the raw material source is controllable, the large-scale production feasibility is high, and the bioactive peptide product can be widely applied to preparation in the fields of functional food, bioactive raw materials and the like.