The invention relates to a Cordyceps sobolifera active site and application thereof in preparing drugs for nerve protection and aging resistance. The active site is a Cordyceps sobolifera alcohol extract ethyl acetate extraction site. The active site is prepared by the following steps: pulverizing the medicinal material Cordyceps sobolifera, screening, extracting with ethanol under reflux, dispersing the ethanol extract with water, sequentially carrying out fractional extraction with petroleum ether, ethyl acetate and n-butanol for 3-5 times, merging, concentrating under reduced pressure to recover the solvent, and carrying out freeze-drying to respectively obtain a petroleum ether site, an ethyl acetate site, an n-butanol site and a water site. The ethyl acetate site can obviously inhibit aging and damage of the glutamic-acid-induced PC12 cells, prevent the cell LDH from release, enhance the survival rate of the cells, lower the intracellular oxygen free radical level, enhance the activity of the glutathione reductase (GSH-Px) and superoxide dismutase (SOD), and have favorable in-vitro oxidation resistance activity in the experiment of clearing DPPH. and superoxide anion (O<2.->) free radicals.