237results about How to "Promote osteogenic differentiation" patented technology

The invention relates to a method for modifying the surface of polyetheretherketone by injecting tantalum ions and a modified polyetheretherketone material. According to the method, the tantalum element containing modified layer is obtained by injecting the tantalum ions to the surface of the polyetheretherketone by adopting a plasma immersion ion injection technology, the polyetheretherketone approaches the cortical bone of a human body by enhancing the elastic modulus and rigidity of the surface of the polyetheretherketone and the biocompatibility and osseointegration property of the surface of the polyetheretherketone are improved. The polyetheretherketone material obtained through the treatment of the modifying method is enhanced in biocompatibility and mechanical property at different degrees. Besides, a bMSC (Bone Marrow Stromal Cells) cell has an obvious osteogenic differentiation trend on the surface of the polyetheretherketone material obtained through the treatment of the modifying method.

The invention discloses a calcium phosphate-based bone repair scaffold and a preparation method thereof. Calcium phosphate bone cement is used as a raw material and is combined with a 3D printing technology to obtain a calcium phosphate bone cement scaffold with through macropores; a dopamine solution is used for modifying the surface of the scaffold; dopamine forms a polydopamine layer on the surface of the scaffold through self-oxidation under alkaline conditions; polydopamine has good viscosity and biocompatibility, can be used as an adhering coating layer of BMP-2, improves the content offixed BMP-2, and promotes cell adhesion and proliferation; the BMP-2 loaded on the scaffold can also effectively promote the proliferation and osteogenic differentiation of bone marrow mesenchymal stem cells. The prepared calcium phosphate-based bone repair scaffold has through macropores, meets the mechanical strength requirements of cancellous bones, has excellent biocompatibility, can effectively promote bone tissue regeneration, and has potential application value in the field of bone tissue repair.

The invention relates to an attapulgite (ATT)-doped PLGA nano-fiber felt, as well as a preparation method and application thereof. The preparation method comprises the following steps: dissolving PLGA in a mixed solvent to obtain a PLGA electrospinning solution; uniformly dispersing ATT in the PLGA electrospinning solution while stirring to obtain a PLGA/ATT electrospinning solution; and electrospinning by adopting the PLGA/ATT electrospinning solution for preparation. Nano-fibers promote human mesenchymal stem cells (hMSC) to be differentiated into bone cells. According to the preparation method provided by the invention, the process is simple, the product is easy to obtain, and the cost of used PLGA and ATT is relatively low; the prepared ATT-doped PLGA nano-fibers have good mechanical properties, blood compatibility and biocompatibility, and have broad application prospects in the fields of pharmaceutical carriers, tissue engineering scaffold materials and the like.

The invention discloses a preparation method of an intra-fibrous biomimetic mineralized collagen membrane capable of promoting osteogenic differentiation of cells. According to the invention, the preparation method comprises the following steps: preparing a collagen solution; pouring the collagen solution into a prefabricated mold, putting the mold into a closed container containing ammonia water,carrying out neutralizing, moving the neutralized solution out and then putting the solution into a constant-temperature box to carry out culturing continuously, and completing self-assembling and forming a gel mode; carrying out cross-linking on the gel to obtain pure collagen gel; and putting the pure collagen gel into a biomimetic mineralization solution and carrying out culturing for a plurality of days to obtain collagen gel with different biomimetic mineralization degrees; and cooling the collagen gel and carrying out freeze-drying on the cooled gel in a freeze dryer to obtain the finalintra-fibrous biomimetic mineralized collagen membrane. According to the invention, intra-fibrous mineralization of the collagen fiber can be formed by biomimetic mineralization; the fiber network structure of the collagen membrane is reserved and utilized while the mechanical property and the biological property of the hydroxyapatite crystal are utilized.

The invention discloses a titanium metal material surface modifying method and a modified titanium metal material obtained through the method. The method is characterized in that zinc ions and magnesium ions are co-implanted to the surface of a titanium metal material through a plasma immersion ion implantation technology in order to improve the biological activity of the medical titanium metal material. The modified titanium metal material has the characteristics of improved corrosion resistance, good cell spreading performance and increased cell proliferation rate, can promote ossification differentiation of rBMSCs, and has an ossification promotion effect.

The invention discloses a 3D printed PCL-PDA-BMP2 bone tissue engineering scaffold and a preparation method thereof. The 3D printed PCL-PDA-BMP2 bone tissue engineering scaffold is prepared accordingto the following steps: adopting a fusion extruding forming type 3D printing technology for extruding PCL and forming fiber bundles and preparing a 3D printed PCL scaffold through a splicing frameworkof the fiber bundles; forming a PDA coating by automatically polymerizing dopamine on the fiber surface of the 3D printed PCL scaffold, thereby preparing a 3D printed PCL-PDA scaffold; lastly, soaking the acquired 3D printed PCL-PDA scaffold in a BMP2 solution, thereby acquiring the 3D printed PCL-PDA-BMP2 bone tissue engineering scaffold. The scaffold prepared according to the invention has theadvantages of simple and reliable structure, controllable appearance and microstructure, reliable mechanical properties and controllable ion release property, and can be used for restorative treatmentof bone trauma, bone tumors and bone defect after bone infection.

The invention discloses a preparation method of an icariin controlled release chitosan/hydroxyapatite composite scaffold material. The method comprises the following steps: (1) preparing a raw material solution; and (2) stirring and mixing a chitosan solution with hydroxyapatite slurry evenly to obtain mixed slurry, dropwise adding a succinic acid icariin solution at the amount of keeping the final concentration of succinic acid icariin in the mixed slurry at 1*10<-5> to 1*10<-2> mol/L, adding a cross-linking agent, adjusting the pH to be 5.0-6.5, further stirring and reacting for 10-120 minutes, centrifuging reaction liquid and removing bubbles after reaction is ended, injecting into a mold, and standing for 10-240 minutes to obtain a gel product; freezing, drying, washing, and freezing and drying again. The composite scaffold material disclosed by the invention is capable of improving the bone repair performance of the scaffold material through proliferation and differentiation of slowly released Ica regulating cells.

The invention discloses a 3D printed PCL-Mg bone tissue engineering scaffold and a preparation method thereof. The 3D printed PCL-Mg bone tissue engineering scaffold is prepared from poly-epsilon-caprolactone PCL and magnesium chloride as raw materials through a 3D printing technology. After being implanted into the human body, PCL as a bioabsorbable material is gradually degraded and releases Mgto promote osteochondral generation. The porous structure of the scaffold can induce bone ingrowth and finally repair bone defects, tumors and bone defects after infection. The 3D printed PCL-Mg bonetissue engineering scaffold has the advantages of simple and reliable structure, controllable shape and microstructure, reliable mechanical property, controllable ion release performance, convenient implantation, small trauma and low cost.

The invention discloses a PEEK false tooth composite. The PEEK false tooth composite is prepared from, by weight, 110-150 parts of polyether-ether-ketone, 10-20 parts of nano calcium silicate, 8-12 parts of hydroxyl hydroxyapatites, 4-8 parts of barium glass short fibers, 15-22 parts of organic solvent, 8-18 parts of refined paraffin, 1-4 parts of co-binding agent, 8-12 parts of binder, 2-6 parts of antibacterial agent, 6-10 parts of carbide and/or 8-15 parts of oxide. The invention further discloses a preparation method of the PEEK false tooth composite. The preparation method includes the steps that firstly, the raw materials of nano calcium silicate, hydroxyl hydroxyapatites, carbide and/or oxide are mixed, the mixture is heated to 150-220 DEG C, the situation is kept for 10-30 min, cooling is carried out, the refined paraffin is added, mixing and stirring are carried out, and after the mixture is crushed ultrasonically, the organic solvent, polyether-ether-ketone, the co-binding agent, the binder and the antibacterial agent are added to be stirred till uniform paste is obtained; finally the barium glass short fibers are added, ball-milling and drying are carried out, and the finished composite powder is obtained.

The invention discloses a preparation method of a bioactive glass-wrapping modified zirconium oxide dental ceramic material. The method comprises the specific steps that a hydrolysable silicon-containing compound, triethyl phosphate and nitrate are dissolved in a solvent, the pH value is adjusted, the mixed solution is subjected to a hydrolytic condensation reaction, and bioactive glass precursorsol is obtained; a zirconium oxide suspension solution which is evenly dispersed in advance is added to the bioactive glass precursor sol and evenly dispersed, the sol is converted into gel through aging, a layer of extremely-thin bioactive glass wraps the peripheries of zirconium oxide particles, and after high-temperature sintering, the bioactive glass-wrapping modified zirconium oxide dental ceramic material is obtained. The zirconium oxide dental ceramic material has good biological activity and excellent mechanical properties, proliferation and osteogenic differentiation of bone marrow mesenchymal stem cells can also be promoted at the same time, and accordingly the material helps to improve the osseointegration capacity; the effect of repairing the dental material is improved, the wider clinical application of the material is promoted, and the material has high social economic value.

The invention discloses a lipidosome-based method for modifying a PEEK (polyetheretherketone) surface with hexadecadrol/minocycline and an application of the method. Lipidosome loaded with hexadecadrol and minocycline is prepared with a thin film dispersion method and then modifies the PEEK surface. The method comprises following steps: (1), hexadecadrol and lipids are dissolved in a methanol and chloroform mixture (1:1, v/v); (2), methanol and chloroform are removed by a rotary vacuum evaporator, and then a lipid membrane is hydrated by a hydration solution; lipidosome loaded with hexadecadrol is obtained by water bath ultrasonic treatment, and is extruded by a porous polycarbonate membrane; dialysis is performed overnight; (3), lipidosome is mixed with a minocycline storage solution, the mixture is shaken, and lipidosome loaded with hexadecadrol/minocycline is obtained, and is stored for standby application at 4 DEG C after being dialyzed overnight; (4), PEEK implant is put in a dopamine solution for reaction, and ultrasonic cleaning is performed; (5), the PEEK implant is added to a hexadecadrol/minocycline lipidosome solution for soaking and is slightly cleaned, and a product is obtained. The obtained product can promote bone repair, regeneration and integration.

The invention discloses a method for using a Pickering emulsion method for producing GelMA macropore hydrogel and application. The method comprises the steps of dissolving GelMA and PEG-4SH in PBS buffer liquid, and stirring a mixture; adding MgO nano-particles after fully dissolving the GelMA and the PEG-4SH, stirring a mixture, and conducting ultrasonic treatment on the mixture; adding dodecaneand a photoinitiator, and using a high-speed homogenizer for intensively stirring a mixture to obtain stable Pickering emulsion; and making the emulsion subjected to UV illumination to form gel, and obtaining the GelMA macropore hydrogel by using ethyl alcohol and water for full wash dialysis. The produced GelMA macropore hydrogel has the advantage of the macropore structure, can promote transportation of nutrient substances, discharging of metabolic products and cell communication, entraps a component containing Mg, has effect of promoting adhesion and proliferation of bone marrow mesenchymalstem cells and promoting osteogenic differentiation, has excellent biocompatibility, can be used as a bone tissue engineering scaffold with excellent performance, and has large clinical application potential in the field of bone repair.

The invention provides a tissue engineering scaffold, a preparation method thereof and a 3D printing ink capable of being directly biologically mineralized. The tissue engineering scaffold comprises ahigh molecular hollow tubular structure, and the surface of the scaffold is uniformly covered with a nano-hydroxyapatite layer with controllable thickness. The tissue engineering scaffold can be widely applied to filling and reconstruction of bone tissues. The preparation method of the tissue engineering scaffold is carried out by virtue of shell-core coaxial spray head 3D printing under bio-safety conditions, and a three-dimensional scaffold stacked by hollow pipes can be efficiently prepared. The scaffold comprises an open macroporous structure and a controllable hollow pipe structure. Compared with straight-through hole scaffolds prepared by virtue of an existing template method, the tissue engineering scaffold has two types of hole structures, and the tubular orientation is controllable. The uniformly-distributed and thickness-controllable nano-hydroxyapatite layer is rapidly mineralized on the surface of a high molecular hollow tubular scaffold, so that the adhesion and multiplication of cells and the osteogenic differentiation can be remarkably improved.

According to the invention, a strong acid acidification method is combined to carry out biological functional modification on an orthopaedic biological material PEEK tablet, so that the orthopaedic biological material PEEK tablet is endowed with biological activity of inhibiting secretion of inflammatory factors while promoting osteogenic differentiation and bone mineralization of bone marrow mesenchymal stem cells. The PEEK surface modification method is simple and low in cost, and a prepared PEEK implant has multiple biological functions and is expected to promote osseointegration in a PEEKorthopaedic implant and reduce loosening-related complications caused by early excessive inflammatory response after the PEEK sheet is implanted into a body.

The invention relates to applications of a non-coding RNA target and an inhibitor of the non-coding RNA target in preparing medicines capable of promoting osteogenic differentiation of mesenchymal stem cells. According to the technical scheme, for the first time, the result shows that the expression level of miR-498 is relevant to osteogenic differentiation of hBMSCs, and the inhibition for the expression level of miR-498 is beneficial for osteogenic differentiation of hBMSCs. Ginsenosides RK1, RG5 and RZ1 can inhibit the expression level of miR-498 and belong to effective inhibitors for expression of miR-498, ginsenosides RK1, RG5 and RZ1 promote the osteogenic differentiation of hBMSCs by inhibiting the expression of miR-498, and ginsenoside RZ1 has the excellent efficacies.

The present invention relates to applications of a bionic silicon-calcium hybrid collagen scaffold material within fiber, specifically to the osteoclastogenesis inhibition application of the bionic silicon-calcium hybrid collagen scaffold material within fiber, and the application of the bionic silicon-calcium hybrid collagen scaffold material within fiber as the bone defect repair material. According to the present invention, calcium ions and silicic acid released by the bionic silicon-calcium hybrid collagen scaffold material within fiber under the body fluid environment can promote osteogenic differentiation of the mesenchymal stem cells and induce the mesenchymal stem cells to secrete a large number of osteoprotegerin so as to provide effects of osteoclast activation inhibiting and bone damage function inhibiting, and in the late stage of implantation, the secretion of the osteoprotegerin returns to the normal level, and the activation of the osteoclasts is beneficial to the normal processing of the bone remodeling process.

The invention relates to the technical field of chemistry, and concretely relates to an application of zinc in the preparation of a bone or joint restoration regeneration material. A zinc modified calcium silicate coating is prepared from ethyl orthosilicate, zinc nitrate and calcium nitrate, the surface of the bone or joint restoration regeneration material is coated with the zinc modified calcium silicate coating to form a zinc modified calcium silicate coating layer, and the coating layer and BMSCs are cocultured. The BMSCs are cultured on the surface of every group material in experiments in the invention, and biological influences of a Ca2ZnSi2O7 ceramic coating layer on the BMSCs are researched through adherence, proliferation and differentiation experiments, so influences of the surface morphology and the chemical components of the zinc modified calcium silicate coating layer and bioactive ions released by the coating layer on the BMSCs are confirmed, and the zinc modified calcium silicate coating layer with a molar ratio of zinc/calcium of 0.3 has a best BMSCs bioactivity promotion effect.

The invention provides a preparation method of a novel tissue repair-promoting material and an application thereof, particularly can be used for bone defect and repair treatment, which belong to the field of tissue engineering and regenerative medicine. A composite scaffold is prepared by mixing and freeze-drying TGF-beta3-chitosan-ossein protein microspheres and chitosan sponge containing bioactive human-like collagen protein. The composite scaffold has the characteristics of being good in biocompatibility, is capable of promoting proliferation and adhesion of cells around injured tissues andcollecting stem cells in vivo, and is suitable for cell growth and osteogenic differentiation and the like. Meanwhile, the periodontal ligament stem cells can be tightly combined with the scaffold material, so that the good growth of the cells in the scaffold material is facilitated, and the scaffold material has stronger osteogenic differentiation capacity and can be better applied to treatmentof bone defects. Wide application prospects are realized in the fields of bone tissue engineering and regenerative medicine, particularly in the aspects of bone defect treatment and repair and the like.

The invention belongs to the technical field of medical biomaterials and particularly relates to composition and a reserve of a bone filling material as well as preparation methods and applications of the composition and the reserve. The composition of the bone filling material comprises bone meal, gelatin and a crosslinking agent, and has the advantages of being inductive, elastic and good in shaping property. The reserve of the bone filling material is a product prepared from a solution of the composition of the bone filling material through freeze drying. A method for preparing the bone filling material comprises the step of mixing the composition or the reserve of the bone filling material with a medically acceptable solvent. The raw materials are widely sourced, the preparation is simple, and a fillable bone filling material can be provided timely during clinical application. The bone filling material can be widely applied to the field of scientific research, or serve as a medical material for use.

The invention relates to a surface modification method for an implant tooth nail by a plasma immersion ion implantation technology. The method comprises exciting a zinc cathode and a silver cathode to respectively serve as a zinc ion plasma source and a silver ion plasma source; and zinc ions and silver ions are injected to the surface of the implant tooth nail by the plasma immersion ion implantation technology for surface modification.