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Method for preparing dual-factor carrying type hybrid bionic bone scaffold and application of dual-factor carrying type hybrid bionic bone scaffold

A bionic bone and dual-factor technology, applied in medical science, prostheses, etc., can solve the problems of uneven mineralization, high brittleness, low osteogenic activity, etc., to induce in situ regeneration of autologous bone tissue, and overcome the acquisition of seed cells Difficulty, effect of promoting osteogenic differentiation

Active Publication Date: 2015-01-21
FOURTH MILITARY MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, when this technology is applied to the demineralized collagen matrix with a hierarchical structure, due to the high density of the natural collagen matrix, the high degree of cross-linking of the collagen, and the many hierarchical levels, the permeability of the mineral precursor to the collagen is poor, making the mineral Long calcification period (14 days to 3 months), uneven mineralization, and inability to form uniform intrafiber calcification of demineralized collagen matrix have become constraints for the further application of biomimetic mineralization technology in the field of bone defect repair materials.
In addition, this kind of intrafiber calcified material has the problems of high brittleness and low bone activity. How to use biomimetic synthesis method to achieve rapid and uniform intrafiber mineralization on the basis of natural collagen multi-level hierarchical structure, while improving Its toughness and promotion of bone activity have become the focus and difficulty of further research in this field

Method used

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  • Method for preparing dual-factor carrying type hybrid bionic bone scaffold and application of dual-factor carrying type hybrid bionic bone scaffold
  • Method for preparing dual-factor carrying type hybrid bionic bone scaffold and application of dual-factor carrying type hybrid bionic bone scaffold
  • Method for preparing dual-factor carrying type hybrid bionic bone scaffold and application of dual-factor carrying type hybrid bionic bone scaffold

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preparation example Construction

[0035] The bone collagen support (natural bone collagen matrix) of the present invention can be animal bone collagen support such as bovine bone collagen support, pig bone collagen support, monkey bone collagen support. The bone collagen scaffold is a demineralized and deantigenated collagen scaffold with a hierarchical structure. The preparation method of the calcium / silicon hybrid biomimetic bone scaffold in multi-grade fibers includes:

[0036] (1) A collagen scaffold with a hierarchical structure was prepared by degreasing, deproteinizing, demineralizing, and deantigenizing methods.

[0037] (2) Obtain an orthosilicate solution by hydrolyzing tetraethyl orthosilicate, and mix it with polypropylene ammonium chloride solution to prepare a stable silicic acid precursor solution;

[0038] (3) Place the demineralized and deantigenated collagen scaffold obtained in step (1) in the silicic acid precursor solution obtained in step (2) for mineralization treatment, so as to realize t...

Embodiment 1

[0046] The method for constructing the dual-factor carrier-type hybrid biomimetic bone scaffold in this embodiment follows the steps below:

[0047] (1) Take bovine metaphyseal cancellous bone to prepare 1cm×1cm×1cm bone fragment; soak 1kg bone fragment in 1L0.1mol / L NaOH solution for 24h, then degrease with 1L1:1 chloroform / methanol mixed solvent for 72h , replace the degreasing solution every 24 hours, and centrifuge at 4000 rpm for 30 minutes in the degreasing interval to remove bone fragments or foreign objects in bone particles; Calcium until completely demineralized; 500mL 4mol / L guanidine hydrochloride, 50mmol / L Tris-HCl and 0.5mol / L CaCl 2 (pH=7.4) solution was deproteinized again at 4°C for 24 hours to completely remove non-collagen proteins with strong immunogenicity, while leaving the collagen structure undamaged; rinse with 500mL double-distilled water, sterilize with 25kGy γ-rays, and sterilize at 4°C Store for later use.

[0048] (2) Use tetraethyl orthosilicat...

Embodiment 2

[0054] This embodiment differs from Embodiment 1 in that:

[0055] (3) Place the demineralized and deantigenated bovine collagen scaffold obtained in step (1) in the silicic acid precursor solution obtained in step (2) for mineralization treatment, replace the fresh silicic acid precursor solution every day, and incubate respectively for 4 Days later, after being washed repeatedly with distilled water, freeze-dried for later use, a biomimetic silicified bone collagen scaffold material with a hierarchical structure was prepared.

[0056] (5) Place the intrafibrous biomimetic silicified bone collagen scaffold material obtained in step (3) in the polyanion-stabilized amorphous liquid-phase calcium phosphate precursor solution obtained in step (4), and incubate at 37°C for 4 days, every day Replace the calcification solution. Finally, a biomimetic hybrid collagen scaffold material with a hierarchical structure is formed, which is characterized by the ordered deposition of hydroxy...

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Abstract

The invention relates to a method for preparing a dual-factor carrying type hybrid bionic bone scaffold and application of the dual-factor carrying type hybrid bionic bone scaffold. The preparation method comprises the following steps: immersing a calcium- silicon hybrid bionic bone scaffold in fibers in a chemotactic factor-osteogenesis inducing protein mixed solution with proper concentration; treating under a proper condition to obtain the dual-factor carrying type hybrid bionic bone scaffold. The related application is application of the scaffold material in a bone defect repairing material. The hybrid ossein contains silicon dioxide and hydroxyapatite two minerals, wherein due to the effective combination of massive negative charge-loaded silanol groups existing on the surface of the silicon dioxide and active hydroxyl radicals on basic protein such as SDF-1 or TGF-beta and the specifically strong adsorptivity of nano-hydroxyapatite on osteogenesis induced protein, such as BMP-2 or BMP-7, an extra sustained-release system need not to be added, and hybrid osteocyte recruitment / osteogenesis induction double functionalized modification can be realized.

Description

technical field [0001] The invention belongs to the field of tissue engineering scaffold materials, and relates to a method for constructing a novel intrafibrous calcium / silicon hybrid biomimetic bone scaffold capable of inducing bone regeneration in situ. Novel biomimetic hybrid bone scaffold materials for regeneration. Background technique [0002] Natural bone tissue is mainly formed by the interaction of collagen organic matter and nano-hydroxyapatite inorganic minerals in a 7-level hierarchical and orderly arrangement, which determines the excellent mechanical properties and biological characteristics of bone tissue. Although there are hundreds of kinds of synthetic bone repair materials at present, there is no product that can accurately simulate the composition, nanostructure and hierarchical structure of natural bone at the same time, which makes it unable to combine the excellent mechanical properties and high biological activity of natural bone. characteristics. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/46A61L27/36A61L27/54A61L27/58
Inventor 牛丽娜焦凯陈吉华郑智明李齐宏陆松鹤
Owner FOURTH MILITARY MEDICAL UNIVERSITY
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