56 results about "Bone collagen" patented technology

This invention discloses a method and application for extracting soluble collagen from bones unearthed from archaeological sites, belonging to the field of archaeological sample processing technology. Specifically: 1) After pretreatment, the blocky ancient bone sample is dried at low temperature and ground into bone powder; the bone powder is added to an acidic solution for ultrasonic treatment, centrifuged, and the supernatant is filtered to obtain the sample to be loaded onto a column; 2) The sample to be loaded onto the column is subjected to chromatographic separation, using a gradient elution with equilibration buffer and elution buffer, and a UV detector is used to monitor protein peaks, collecting the target protein solution that elutes within 20-30 minutes; 3) Finally, the target protein solution is concentrated and purified using an ultrafiltration tube to obtain soluble collagen. This invention can obtain a sufficient amount of collagen from ancient deteriorated bone samples, significantly improving sample utilization and providing reliable technical support for bioarchaeological research.

The invention discloses a preparation method of collagen micromolecule active peptide and bioactive peptide for inhibiting cancer cell proliferation and improving intracellular environment, and belongs to the technical field of bioactive peptide preparation and biological functional raw materials. According to the method, bone collagen peptide, cartilage peptide (hydrolyzed II-type collagen peptide), collagen peptide (German Jilida), non-denatured II-type collagen peptide and yak bone marrow peptide are taken as core raw materials, and optional ingredients such as bovine bone marrow peptide powder, shark cartilage peptide and Belgian imported inulin can be compounded as required; through a step-by-step preparation process comprising the steps of graded purification pretreatment of raw materials, precise proportioning and mixing, low-temperature activation and synergism and stable micro-capsule embedding, structure protection and efficacy synergy of each collagen small-molecule active peptide and bioactive components are realized; the prepared bioactive peptide product can significantly inhibit cancer cell proliferation, regulate intracellular oxidation-reduction balance and improve intracellular microenvironment, the preparation process is mild and free of high-temperature damage, the raw material biological activity retention rate is high, the raw material source is controllable, the large-scale production feasibility is high, and the bioactive peptide product can be widely applied to preparation in the fields of functional food, bioactive raw materials and the like.

This application discloses a method and its application for preparing collagen peptides by stepwise enzymatic hydrolysis with dynamic pH control, belonging to the field of protein preparation technology. The method includes raw material pretreatment, stepwise enzymatic hydrolysis with dynamic pH control, and post-treatment to obtain collagen peptide products with a molecular weight distribution of 800-2000 Da. The obtained products can be used to prepare products with bone growth promotion functions. This application employs a combined process of stepwise enzymatic hydrolysis with dynamic pH control, alkali-thermal coupling pretreatment, and 2000 Da retention. The steps work synergistically: alkali-thermal treatment exposes enzyme cleavage sites, dynamic pH control enables efficient enzymatic hydrolysis, and 2000 Da retention of active components results in a significantly higher yield and content of collagen peptides compared to conventional processes. Animal experiments have demonstrated that the collagen peptides have bone growth promotion activity, and the mechanism of action has been revealed at the IGF-1 signaling pathway level.

The invention belongs to the technical field of food preparation, and particularly relates to a biological information technology guidance-based compound bovine bone collagen peptide beverage, which is prepared from the following raw materials: bovine bone collagen peptide powder, red date polysaccharide, astragalus polysaccharide, calcium carbonate, chondroitin sulfate and sodium hyaluronate. When the compound bovine bone collagen peptide beverage is drunk, bovine bone contains a large amount of collagen, is rich in type I collagen and is richer in amino acid composition, and a large number of research results show that protein is not completely hydrolyzed into amino acid after being ingested, most of the protein is absorbed in a peptide form, and the protein is absorbed in a peptide form. Therefore, the selected bovine bone collagen peptide powder can be better absorbed by the human body. The red dates have the effects of maintaining beauty, keeping young and resisting aging. In addition, astragalus membranaceus and other traditional Chinese medicine extracts are supplemented in the compound bovine bone collagen peptide beverage, so that the functional characteristics of the beverage are enhanced, and the original bad flavor of animal peptide is improved.

The application discloses a method for determining key amino acid residues of bone collagen peptide promoting osteogenesis activity, belongs to the field of biological medicine and food science, and aims to solve the technical problem that traditional methods are low in efficiency, insufficient in accuracy, and cannot efficiently identify key amino acid residues of bone collagen peptide determining the activity of promoting osteogenesis. The application comprises the following steps: firstly, obtaining target bone collagen peptide and constructing a single-point alanine mutant peptide library thereof; then, sequentially performing conformation and physicochemical property characteristic analysis, binding free energy simulation calculation with specific receptor protein, and cell experiment determination of the activity of promoting osteogenesis on the target peptide and the mutant peptide; finally, screening the key amino acid residues based on multi-level joint determination of characteristic deviation, binding free energy change and activity decline ratio. The application is mainly used for quickly and accurately positioning the core functional site of bone collagen peptide promoting osteogenesis activity, and provides key theory and technical basis for efficient screening, rational design and function strengthening of osteogenic activity peptide.

The invention discloses a method for preparing a phosphorylated salmon collagen peptide-calcium chelate by taking salmon collagen peptide as a raw material, preparing high-calcium binding active peptide (P-CP) through phosphorylation modification and chelating the high-calcium binding active peptide with calcium ions. The method comprises the following steps: (1) enzymolysis preparation of salmon bone collagen; (2) phosphorylation modification of a peptide fragment mediated by sodium tripolyphosphate; and (3) optimizing a calcium ion chelation process. The chelating rate of the obtained P-CP-Ca calcium reaches 91.21%, and structural characterization proves that a phosphate group (PO4 < 2->) is combined with calcium ions through ionic bonds. Animal experiments show that P-CP-Ca can significantly improve the serum calcium level of a calcium-deficiency mouse (the H group recovers to a normal range), reduce the activity of a bone resorption marker TRAP (Target Related Amplified Protein) (which is 25.4% lower than that of a model group), and improve the bone trabecula microstructure (the bone mineral density is improved by 32.7%). The invention provides a new technology for valuable utilization of fishery by-products and development of functional calcium supplements.

The application discloses a composition capable of effectively inhibiting the growth of Staphylococcus aureus biofilm. The application firstly optimizes the nitrogen source in the culture medium of Staphylococcus epidermidis CCSM0287 strain, fully explores the growth difference of Staphylococcus epidermidis under different culture media and culture conditions and the inhibition effect of the fermentation supernatant on Staphylococcus aureus biofilm, then compounds the fermentation supernatant of Staphylococcus epidermidis CCSM0287 obtained by using the optimized culture medium (the nitrogen source adopts tryptone + soybean peptone + fresh beef bone collagen peptide powder) with the fermentation supernatant of Lactobacillus paracasei S20, can obviously inhibit the growth of Staphylococcus aureus biofilm and destroy the surface structure of the Staphylococcus aureus biofilm, and can be applied in bacteriostatic products.

The invention discloses a composition containing bovine bone collagen as well as a preparation method and application thereof, and belongs to the technical field of collagen application. The composition containing the bovine bone collagen is obtained by chemical crosslinking of bovine bone collagen magnesium chelate, sodium hyaluronate, chondroitin sulfate and bovine bone collagen through EDC / NHS. The composition containing bovine bone collagen is prepared from the following components in parts by weight: 5 to 10 parts of bovine bone collagen magnesium chelate, 2 to 5 parts of sodium hyaluronate, 0.5 to 2 parts of chondroitin sulfate and 20 to 30 parts of bovine bone collagen. The bovine bone collagen magnesium chelate, the sodium hyaluronate, the chondroitin sulfate and the bovine bone collagen in the composition containing the bovine bone collagen prepared by the invention have the characteristics of good biocompatibility, capability of promoting cell proliferation and differentiation into cartilage cells, and suitability for cartilage repair.

The invention belongs to the technical field of protein beverages, and particularly relates to a composite peptide beverage and a preparation process thereof. The active ingredients of the drink comprise calf thymosin, cattle spleen protein peptide, hemoglobin peptide, bone collagen peptide and collagen peptide in a weight ratio of (2-3): 1: 1: 1: 1. The pH value of the system is adjusted to 3.5-4.5, so that each peptide molecule is positively charged under the acidic condition, the electrostatic repulsion and hydration among molecules are enhanced, and bitter groups are effectively masked. The flavoring agent is compounded by DL-malic acid, crystalline fructose, xylitol and sucralose according to the weight ratio of (0.6-1): 1: 0.4: (0.03-0.04), and the sour and sweet taste and flavor coordination are synergistically improved. The preparation method comprises the following steps: dissolving the DL-malic acid in purified water, dissolving the uniformly mixed active components in purified water at 40-60 DEG C, respectively adding other flavoring agents, stabilizing agents and essence, and finally filtering to obtain the product. The problem of bad flavor of the peptide is solved, so that the beverage is appropriate in taste, harmonious in flavor and easy to accept.

The application provides king salmon bone collagen peptide for promoting longitudinal growth of bone, a preparation method and application, and relates to the technical field of collagen peptides. The preparation method comprises the following steps: (1) treating king salmon bone raw materials by steam explosion to obtain pretreated king salmon bone; then mixing the pretreated king salmon bone with pepsin and performing first enzymolysis under pressure to obtain a first enzymolysis mixture; mixing the first enzymolysis mixture with collagenase and ficin, performing second enzymolysis under pressure, collecting an enzymolysis liquid, and performing ultrafiltration on the enzymolysis liquid to obtain collagen peptide. In the application, pepsin is used for first enzymolysis under pressure, then collagenase and ficin are used for compounding, the synergistic effect of the three kinds of proteases makes the collagen be fully enzymolyzed, the diversity of the cleavage site is improved, the molecular weight of the polypeptide is reduced, the activity of the polypeptide is improved, the longitudinal growth of the bone is promoted, and the bone density is improved.

The invention provides a high-purity bovine bone collagen peptide gel filtration chromatography purification method, and belongs to the technical field of bioactive peptide purification. The method comprises the following five core steps: pretreatment, gel column preparation, dynamic equilibrium, sample loading and elution, and post-treatment: firstly, carrying out ultrafiltration pretreatment on a bovine bone collagen peptide crude extract, then filling a chromatographic column with a specific diameter-height ratio by using cross-linked sephadex, carrying out dynamic equilibrium by using an accurately adapted equilibrium liquid after column efficiency verification, and finally, carrying out dynamic equilibrium by using a chromatographic column with a specific diameter-height ratio. The sample loading amount is dynamically matched through a formula, a gradient isothermal mode is adopted in the elution process, and finally a high-purity product is obtained through freeze drying. Through multi-step parameter collaborative optimization and precise regulation and control of a self-created formula, the problems of low purification efficiency, poor product purity and wide molecular weight distribution in the prior art are solved, the purity of the obtained bovine bone collagen peptide is greater than or equal to 98%, the proportion of peptide fragments with molecular weight distribution of 500-3000Da and 1000-2000Da is greater than or equal to 70%, the heavy metal content and the colony count both meet high-standard requirements, the yield is stabilized at 52% or above, and the method is suitable for industrial production. Good industrial application prospects are realized.

The application discloses an active peptide for promoting bone growth, and a preparation method and application thereof. The active peptide contains amino acid sequences GPAGPLGPV, LGVPLPL, FLPEPPQEE and SFLPEPPQE, and is derived from an enzymatic product of animal bone collagen. The average molecular weight of the enzymatic product is 500-1000 Da, and the mass percentage of components with a molecular weight less than 1000 Da is greater than 60%. The preparation method comprises the following steps: sequentially performing acid treatment, alkali treatment, extraction, filtration and ion exchange on animal bone, and then performing enzymolysis on the animal bone under the action of a protease to obtain an enzymolysis solution, wherein the protease is selected from alkaline protease, trypsin and papain; and the enzymolysis solution is separated and purified, wherein the separation and purification comprises ultrafiltration membrane grading and chromatographic separation, the cut-off molecular weight of the ultrafiltration membrane is 3kDa, and the chromatographic separation comprises gel chromatography and reverse-phase high performance liquid chromatography separation. The low-molecular active peptide obtained by the application can promote the proliferation and / or differentiation of osteoblasts, improve the alkaline phosphatase activity, has a small molecular weight and is easy to be absorbed and utilized by the body, has a wide raw material source, has a simple and controllable preparation process, and has a good application prospect.

This invention belongs to the field of genetic engineering technology and discloses a high-efficiency expression method for recombinant collagenase and its application in the preparation of bovine bone collagen peptides. The collagenase gene derived from Bacillus sp. HUB-I-004 strain (accession number J9CTX3) was optimized using E. coli-preferred codons. A recombinant expression vector was constructed and transformed into E. coli BL21(DE3). After optimization of induction conditions and Ni-NTA affinity purification, a highly active recombinant collagenase with a specific activity of 231.05 U / mg was obtained. Bovine bone collagen was extracted using a high-temperature, high-pressure method. Bovine bone collagen peptides were prepared under optimal conditions of a substrate concentration of 10 mg / mL, an enzyme addition of 20%, and enzymatic hydrolysis at 37℃ for 8 h, significantly improving peptide yield. The obtained product is mainly composed of small molecule peptides and exhibits significant osteoblast proliferation-promoting activity, making it widely applicable in the preparation of health foods and pharmaceuticals.

The application discloses narrow hake bone collagen protein peptide and a preparation method and application thereof, and belongs to the technical field of biotechnology. The preparation method of the narrow hake bone collagen protein peptide comprises the following steps: after narrow hake bones are crushed, the narrow hake bones are added with ultrapure water to obtain a mixture, a compound protease composed of alkaline protease and trypsin is added into the mixture to perform enzymolysis, the enzymolysis liquid is filtered through a microporous filter membrane to obtain an enzyme hydrolysate; the enzyme hydrolysate is separated through a 3k Da ultrafiltration membrane, components with a molecular weight less than 3kDa are collected, and after being suction filtered, the narrow hake bone collagen protein peptide is obtained through gel chromatography separation. The narrow hake bones are used as raw materials, three polypeptides with anti-fatigue activity and anti-inflammatory activity are prepared and screened, the polypeptides are artificially synthesized, a narrow hake bone collagen protein anti-fatigue and anti-inflammatory activity peptide chewable tablet product is developed, and reference bases are provided for high-value utilization of aquatic product processing byproduct protein resources.

The invention belongs to the technical field of functional protein peptides, and particularly discloses a chicken bone collagen peptide-selenium nano-composite and a preparation method and application thereof, and the method comprises the following steps: extracting and degreasing a chicken skeleton of a black chicken in old hospital; removing non-collagen protein, fat and mineral substances in the chicken bone protein freeze-dried powder; preparing the chicken bone collagen peptide from the chicken bone collagen freeze-dried powder in an environment of simulating gastrointestinal tract digestion; the chicken bone collagen peptide-selenium nano compound is prepared by taking chicken bone collagen peptide and sodium selenite as raw materials. According to the chicken bone collagen peptide-selenium nano-composite and the preparation method and application thereof, the chicken bone of the black chicken in the old hospital is taken as a raw material, and the prepared chicken bone collagen peptide-selenium nano-composite has the characteristics of high absorptivity, high safety and strong oxidation resistance; furthermore, the chicken bone collagen peptide-selenium nano compound can be widely applied to the future food or pharmaceutical field as a high-efficiency dietary organic selenium supplement.

The invention discloses a special diet for improving cardiovascular and cerebrovascular diseases and microcirculation disturbance and a preparation method thereof, and the special diet comprises the following components in parts by weight: 2-4 parts of bone collagen peptide, 1-2 parts of taurine, 2-3 parts of hydrolyzed collagen peptide, 1-2 parts of marine fish oligopeptide, 1-2 parts of inulin, 1-2 parts of erythritol, 2-3 parts of acerola cherry fruit powder and 2-3 parts of guarana extract. 1-2 parts of N-acetylneuraminic acid, 1-2 parts of vitamin C, 1-2 parts of vitamin B6, 1-2 parts of vitamin B1 and 1-2 parts of vitamin B2; the traditional Chinese medicine composition can improve cardiovascular functions and microcirculation disturbance, and has remarkable effects in the aspects of inhibiting cardiovascular diseases, promoting microcirculation and enhancing immunity.

The invention relates to the technical field of collagen peptide extraction, and particularly discloses a preparation method of fresh bovine bone collagen peptide for increasing bone mineral density, which comprises the following steps: step S1, degreasing treatment; step S2, crude extraction; step S3, performing enzymolysis; step S4, performing supercritical extraction; according to the technical scheme, the extraction promoting liquid is added in the crude extraction process, meanwhile, ultrasonic-assisted synchronous microwave radiation is matched, calcium lactate, lecithin, chlorogenic acid and compound enzyme are added in the enzymolysis process, and the purity and yield of the fresh bovine bone collagen peptide are improved through the synergistic effect of all the procedures.

The invention discloses a dietary therapy formula for repairing damaged cells and promoting cranial nerve development and a preparation method, and the dietary therapy formula comprises the following components in parts by weight: 1-2 parts of bone collagen peptide, 1-2 parts of taurine, 1-2 parts of bovine bone marrow powder, 2-4 parts of inulin, 2-4 parts of acerola cherry fruit powder, 3-4 parts of guarana extract and 4-6 parts of N-acetylneuraminic acid. 4-5 parts of vitamin B6, 2-4 parts of vitamin B1 and 2-4 parts of vitamin B2; the traditional Chinese medicine composition is capable of repairing damaged cells and promoting cranial nerve development, and has remarkable effects of inhibiting the onset of neurological diseases, promoting the repair of nerve cells and promoting nerve development.

The invention provides a bone collagen peptide for increasing bone mineral density and a preparation method thereof, the method comprises the following steps: S1, dispersing bone gelatin in water to obtain a bone gelatin solution; s2, adding a 0.1-1 mol / L acidic pH regulator into the bone gelatin solution at a speed of 1-10 mL / min until the turbidity of the system is increased by 10-20% compared with the turbidity of the initial bone gelatin solution, then stopping adding, standing for 10-30 min, and filtering to obtain a bone gelatin supernatant; s3, adding endoprotease into the bone gelatin supernate to obtain enzymatic hydrolysate; and S4, performing post-treatment and drying on the enzymatic hydrolysate to obtain the bone collagen peptide. According to the method, the mass ratio of the low-molecular-weight polypeptide in the obtained bone collagen peptide can be increased, and the composition of the obtained bone collagen peptide is more suitable for bone metabolism to increase the bone density.

The invention discloses a dietary therapy formula for repairing damaged cells and promoting cranial nerve development and a preparation method, and the dietary therapy formula comprises the following components in parts by weight: 1-2 parts of bone collagen peptide, 1-2 parts of taurine, 1-2 parts of bovine bone marrow powder, 2-4 parts of inulin, 2-4 parts of acerola cherry fruit powder, 3-4 parts of guarana extract and 4-6 parts of N-acetylneuraminic acid. 4-5 parts of vitamin B6, 2-4 parts of vitamin B1 and 2-4 parts of vitamin B2; the traditional Chinese medicine composition is capable of repairing damaged cells and promoting cranial nerve development, and has remarkable effects of inhibiting the onset of neurological diseases, promoting the repair of nerve cells and promoting nerve development.

This invention relates to a method for preparing an immune-enhancing eel bone peptide composition, belonging to the field of bioextraction technology. The method includes the following steps: a) Pretreatment: Eel bone material is sequentially freeze-dried at low temperature, pulverized to below 200 mesh, defatted with ethanol, and delimed using acid methods to obtain pretreated bone material; b) Three-stage complex enzymatic hydrolysis: The pretreated bone material is subjected to three-stage complex enzymatic hydrolysis to obtain an enzymatic hydrolysate; c) Enzyme inactivation and centrifugation: The enzymatic hydrolysate is inactivated and then centrifuged to remove insoluble matter, obtaining a clear liquid; d) Ultrafiltration separation: The clear liquid is subjected to ultrafiltration to remove macromolecular impurities, obtaining a collagen peptide concentrate; e) Debittering, deodorizing, and drying: The collagen peptide concentrate is debittered and deodorized, then dried to obtain eel bone collagen peptide powder. This method effectively removes fishy and bitter tastes, significantly improving the product's bioactivity, purity, and palatability.

This invention provides a bone regeneration formula based on collagen synergy and its preparation method. The formula uses low-molecular-weight collagen peptides as organic templates and forms hydroxyapatite-like nanostructures through ion-induced mineralization, achieving a tight organic-inorganic interface. The formula can regulate osteoblast differentiation and inhibit osteoclast activity, thereby improving bone density and bone tissue regeneration capacity. The preparation process adopts a low-temperature enzymatic hydrolysis and biomimetic mineralization coupling process to maintain the stability of the active structure. Experimental results show that the formula is superior to conventional formulas in terms of molecular expression, mineralization capacity, and bone metabolism regulation.

The invention belongs to the technical field of animal foods, and relates to a nutritional food for improving joint health of pets and a preparation method thereof. The nutritional food comprises the following raw materials: sweet potato powder, chicken powder, enzymolysis chicken, chicken oil, grains, beef powder, starch, fruit and vegetable powder, chicken liver powder, whole goat milk powder, fish oil, linseed oil, yolk powder, vitamin E, sodium hyaluronate, bovine bone collagen peptide, glucosamine hydrochloride, chondroitin sulfate, dimethyl sulfone, a vitamin mineral premix and a Chinese herbal medicine premix. The nutritional food for improving joint health of pets provided by the invention is rich in nutrition, good in palatability and simple in production process, does not lose nutrition of the raw materials, and promotes and improves digestibility of the nutritional food. Animal experiments show that the nutritional food for improving the joint health of the pets has the advantages that the Lequesne score of canine arthritis is reduced, the levels of IL-1beta and TNF-alpha in canine serum are reduced, the level of CTXII in the canine serum is reduced, and the canine osteoarthritis is effectively prevented and treated.

The present invention discloses a fish maw collagen peptide, its preparation method, and its application in functional beverages, belonging to the field of marine bioactive ingredient extraction and application. Through the steps of softening, deodorising, enzymatic hydrolysis, deodorization, refining, and concentration, the present invention produces a low-molecular-weight fish maw collagen peptide. This peptide is then combined with anthocyanins, vitamin C, concentrated fruit juice, and excipients to formulate a product suitable for consumption as a beverage. Experimental results demonstrate that the fish maw collagen peptide and its product prepared according to the present invention can significantly improve indicators such as skin hydration, elasticity, and firmness, exhibit outstanding reparative effects on gastrointestinal mucosa, and demonstrate a synergistic effect among the fish maw collagen peptide, anthocyanins, and vitamin C. The product exhibits high sensory acceptability, safety, stable quality, and meets the requirements for large-scale production and market promotion.

This application provides a collagen peptide for increasing bone density and a method for preparing the same. The method includes the following steps: S1: dispersing bone gelatin in water to obtain a bone gelatin solution; S2: adding 0.1-1 mol / L acidic pH adjuster to the bone gelatin solution at a rate of 1-10 mL / min until the turbidity of the system increases by 10%-20% compared to the initial bone gelatin solution, then stopping the addition, allowing it to stand for 10-30 min, and filtering to obtain a bone gelatin supernatant; S3: adding an endopeptide to the bone gelatin supernatant to obtain an enzymatic hydrolysate; S4: post-processing and drying the enzymatic hydrolysate to obtain collagen peptides. This method can increase the mass ratio of low molecular weight peptides in the obtained collagen peptides, resulting in a composition of collagen peptides more suitable for bone metabolism and utilization to increase bone density.

The application discloses a euphausia superba oil cartilage extract, a euphausia superba oil cartilage capsule and a preparation method. The euphausia superba oil cartilage extract is composed of the following raw materials in percentage by weight: cartilage collagen 40-60%; non-denatured type II collagen 0-5%; chicken cartilage extract 1-10%; chitosan oligosaccharide 0-10%; and the rest is euphausia superba oil. The compound formula design combines the euphausia superba oil, cartilage collagen, non-denatured type II collagen, chicken cartilage extract and chitosan oligosaccharide and other beneficial ingredients, which can produce positive effects on human cardiovascular health, joint health, skin health, immune system health and intestinal health and other aspects, meet the needs of human body in different physiological and health demands, help to improve the quality of life and promote health.

This invention discloses a Hu sheep active peptide complex and its application in bone health care. Using healthy Hu sheep bones as raw material, collagen is extracted after defatting and decalcification. This collagen is then enzymatically hydrolyzed with trypsin and alkaline protease, followed by ultrafiltration, desalting, and freeze-drying to obtain Hu sheep bone collagen peptides. These collagen peptides are mixed with pharmaceutical excipients, calcium source compounds, chondroprotective agents, and fat-soluble vitamins. An ethanol-water binder solution containing palmitoyl aspartate diethyl ester and hesperidin-3-O-glucoside is added, and the mixture is granulated, dried, sized, and pressed to obtain the active peptide complex. This complex, through the synergistic effect of its components, can significantly promote osteoblast proliferation and inhibit apoptosis, effectively increasing bone density in osteoporosis model animals. It also exhibits excellent biocompatibility and no cytotoxicity, solving the problems of limited function, insufficient preparation efficiency, and instability of existing single active peptides. This makes it suitable for the development and application of bone health-related nutritional supplements.

The invention relates to an amniotic fluid cell culture medium and a preparation method thereof, and belongs to the technical field of cell culture mediums. The culture medium comprises a basic culture medium, a fermented animal polypeptide compound and auxiliary components, the fermented animal polypeptide compound is formed by compounding fermented bovine bone collagen peptide and fermented sheep placenta peptide according to a specific proportion, and polypeptide is subjected to bacillus subtilis fermentation, enzymolysis and purification and then passes through a 0.22 mu m polyether sulfone filter membrane terminal for sterilization. By utilizing the synergistic effect of the fermented animal polypeptide, the proliferation rate and metabolic activity of the amniotic fluid cells are effectively improved, meanwhile, stable physical and chemical properties, standard sterility, definite components, small batch difference and no need of adding fetal calf serum and plant-derived components are ensured, and the method is suitable for amniotic fluid cell culture in prenatal diagnosis.

The invention discloses a bone collagen peptide-zinc-grifola frondosa polysaccharide chelate as well as a preparation method and application thereof, and belongs to the technical field of biology. According to the invention, a multi-dimensional chelation technology is innovatively adopted, and through the synergistic effect of metal ion bridging and polysaccharide space wrapping, peptide-metal-polysaccharide multi-element coordination binding is realized, the bone collagen peptide-zinc-grifola frondosa polysaccharide chelate is constructed, and the dual targets of structure stabilization and function enhancement are realized. According to the bone collagen peptide-zinc-grifola frondosa polysaccharide chelate, the anti-degradation stability of bone collagen peptide in gastrointestinal tracts is remarkably improved, release and degradation of the bone collagen peptide are delayed, and the intestinal retention time of active peptide is prolonged; meanwhile, the biological activities of oxidation resistance, immunoregulation and the like are enhanced, a brand new technical path is provided for developing efficient and stable bone collagen peptide functional products, the bone collagen peptide is expected to be widely applied to the fields of food, nutritional supplements, medicines and the like, and better health guarantee is brought to consumers.