134 results about "Bone collagen" patented technology

The invention discloses a composition for promoting cartilage repair and maintaining joint health as well as a preparation process and application thereof, and relates to the technical field of functional foods. The composition is prepared from the following components in parts by weight: 21.1 to 90 parts of a multi-protein complex, 0.1 to 3 parts of cherry powder, 0.01 to 2 parts of N-acetylglucosamine, 0.01 to 2 parts of radix puerariae powder, 0.1 to 1.5 parts of a calcium compound and 0.01 to 0.2 part of rhizoma curcumae longae, the multi-protein compound is prepared from 0.1 to 10 parts of ray collagen peptide, 1 to 30 parts of bone collagen peptide powder and 20 to 50 parts of fish collagen peptide. The composition provided by the invention can promote cartilage repair, achieves the purpose of daily maintenance of joint health, and has a good application prospect.

The invention discloses a lactobacillus rhamnosus capable of improving joint inflammation and promoting bone health, a metagen and application of the lactobacillus rhamnosus, the lactobacillus rhamnosus is lactobacillus rhamnosus BN02, the lactobacillus rhamnosus is preserved in China General Microbiological Culture Collection Center on November 27, 2023, and the preservation number is CGMCC No.29133. The lactobacillus rhamnosus can be used for improving joint inflammation and promoting bone health. The lactobacillus rhamnosus BN02 can reduce the number of gram-negative bacteria in intestinal tracts of arthritis patients, increase the synthesis amount of bone collagen, and is beneficial to promoting absorption of vitamin C by the intestinal tracts, improving joint inflammation and promoting bone health.

This invention provides a composition for improving bone and joint health, its preparation method, and its application, relating to the field of bone and joint health products technology. By weight, the composition for improving bone and joint health comprises the following components: 0.5-5 parts collagen peptides, 5-30 parts mangosteen extract, 0.5-3 parts non-denatured type II collagen peptides, 0.1-2 parts milk mineral salts, 0.3-3 parts bone collagen peptides, 0.1-2 parts cartilage extract, 0.1-2 parts N-acetylglucosamine, 0.1-2 parts sodium hyaluronate, and 0.3-3 parts turmeric extract. This invention, through multiple combinations of mangosteen extract and turmeric extract, and cartilage extract and sodium hyaluronate, obtains a composition that is low in cost, highly safe, and has a good effect on the prevention or improvement of bone and joint diseases.

The invention discloses a yak bone collagen peptide for improving osteoporosis and preparation and application thereof, and relates to the field of biological medicine, the yak bone collagen peptide comprises collagen peptide with the molecular weight of 500-2000 Daltons, contains a glycine-proline-hydroxyproline tripeptide core structure, can promote osteoblast differentiation and inhibit osteoclast activity, and can be used for treating osteoporosis. The amino acid sequence of the collagen peptide is any one of the following sequences: glycine-proline-hydroxyproline-glycine-glutamic acid-arginine, and the amino acid sequence of the collagen peptide is any one of the following sequences: glycine-proline-hydroxyproline-glycine-glutamic acid-arginine; and the amino acid is glycine, proline, hydroxyproline, serine, leucine and alanine. The yak bone collagen peptide has a unique glycine-proline-hydroxyproline tripeptide structure, the molecular weight is controlled within the range of 500-2000 Daltons, the structure can promote osteoblast differentiation and inhibit osteoclast activity at the same time, and the problem that a traditional osteoporosis treatment medicine is single in function is solved.

The invention provides a preparation method of yak bone collagen peptide, a composition for promoting skeletal development and application of the composition, and relates to the technical field of health food. The preparation method comprises the following steps: carrying out ultrasonic pretreatment on yak bones, and degreasing through supercritical CO2 to obtain a degreased product; mixing the degreased product with a fishy smell removing agent to obtain a fishy smell removing product; the fishy smell removing agent is an aqueous solution of a plant extract; the plant extract is selected from at least one of amomum tsao-ko extract, turmeric extract, green tea extract or momordica grosvenori extract; the fishy smell removal product is subjected to an enzymatic hydrolysis reaction through an immobilized compound enzyme reactor, wherein the compound enzyme is prolyl endopeptidase, papain and ficin; obtaining a crude product; carrying out ultrafiltration by adopting a filter membrane with the molecular weight cut-off of 1kDa, and adsorbing through macroporous resin. The yak bone collagen peptide and the composition prepared by the invention can obviously improve the body length, promote the skeletal development and promote the secretion of growth hormone, and meet the growth requirements of children.

The invention relates to the field of food, in particular to colostrum basic protein as well as a preparation method and application thereof. The colostrum basic protein of the present invention can increase bone mass, increase bone density, promote skeletal development, promote skeletal regeneration or repair, promote calcium absorption, promote bone collagen production, and / or prevent and / or treat bone related diseases (such as osteoporosis, osteoporosis, rickets, osteoarthrosis).

The invention discloses a cod bone-derived high-activity calcium chelating peptide and a preparation method thereof, and belongs to the technical field of functional biological product preparation. The calcium chelating peptide provided by the invention is derived from a cod bone collagen enzymatic hydrolysate and is obtained through affinity chromatography separation of hydroxyapatite, the calcium chelating activity is 4.9-5.2 mu g / mg, and the calcium chelating peptide contains aspartic acid and glutamic acid enriched peptide fragments, through deep processing of cod bones, high-valued utilization of cod bone resources is realized, the high-activity calcium chelating peptide is prepared, and the calcium chelating peptide has a wide application prospect. And a high-quality raw material is provided for the development of a novel calcium supplement.

This invention discloses a method and application for extracting soluble collagen from bones unearthed from archaeological sites, belonging to the field of archaeological sample processing technology. Specifically: 1) After pretreatment, the blocky ancient bone sample is dried at low temperature and ground into bone powder; the bone powder is added to an acidic solution for ultrasonic treatment, centrifuged, and the supernatant is filtered to obtain the sample to be loaded onto a column; 2) The sample to be loaded onto the column is subjected to chromatographic separation, using a gradient elution with equilibration buffer and elution buffer, and a UV detector is used to monitor protein peaks, collecting the target protein solution that elutes within 20-30 minutes; 3) Finally, the target protein solution is concentrated and purified using an ultrafiltration tube to obtain soluble collagen. This invention can obtain a sufficient amount of collagen from ancient deteriorated bone samples, significantly improving sample utilization and providing reliable technical support for bioarchaeological research.

The invention discloses a method for enriching bone collagen osteogenic active peptides based on affinity adsorption, including: installing a hydroxyapatite pre-packed column in an AKTA pure protein purification system, and enriching the bone collagen peptide solution by affinity adsorption through a hydroxyapatite column-AKTA pure protein purification system, wherein, firstly, eluting with mobile phase a as an eluting solution, collecting samples when an absorption peak occurs, and obtaining a bone collagen osteogenic active peptide sample F1, wherein the mobile phase a is a potassium chloride solution. The present invention screens out polypeptides affinity-adsorbed with a hydroxyapatite column matrix, obtains bone collagen peptides with strong ability to bind hydroxyapatite, measures the calcium binding capacity of the bone collagen peptide components obtained by enrichment and separation, and its effect on osteoblast proliferation, and provides technical support for the enrichment and preparation of affinity-adsorbed bone collagen osteogenic active peptides, which is of great significance to improving product effectiveness.

The invention discloses a preparation method of collagen micromolecule active peptide and bioactive peptide for inhibiting cancer cell proliferation and improving intracellular environment, and belongs to the technical field of bioactive peptide preparation and biological functional raw materials. According to the method, bone collagen peptide, cartilage peptide (hydrolyzed II-type collagen peptide), collagen peptide (German Jilida), non-denatured II-type collagen peptide and yak bone marrow peptide are taken as core raw materials, and optional ingredients such as bovine bone marrow peptide powder, shark cartilage peptide and Belgian imported inulin can be compounded as required; through a step-by-step preparation process comprising the steps of graded purification pretreatment of raw materials, precise proportioning and mixing, low-temperature activation and synergism and stable micro-capsule embedding, structure protection and efficacy synergy of each collagen small-molecule active peptide and bioactive components are realized; the prepared bioactive peptide product can significantly inhibit cancer cell proliferation, regulate intracellular oxidation-reduction balance and improve intracellular microenvironment, the preparation process is mild and free of high-temperature damage, the raw material biological activity retention rate is high, the raw material source is controllable, the large-scale production feasibility is high, and the bioactive peptide product can be widely applied to preparation in the fields of functional food, bioactive raw materials and the like.

The present invention belongs to the technical field of new materials, and particularly relates to a fish gelatin polypeptide nanomaterial prepared based on a self-condensation reaction and its application. The fish gelatin polypeptide nanomaterial has a core-shell structure, wherein: the core layer is hydrolyzed tannin, and the shell layer is calcium ions and fish gelatin polypeptide; the hydrolyzed tannin is connected to the calcium ions through a coordination bond, and the hydrolyzed tannin is connected to the fish gelatin polypeptide through a hydrogen bond. The present invention adopts a one-step method to cause a self-condensation reaction of fish gelatin polypeptide, hydrolyzed tannin and calcium salt in a polar organic solvent, forming a "core-shell" structure with calcium ion-polypeptide on the outside as the shell and hydrolyzed tannin on the inside as the core. The fish gelatin polypeptide nanomaterial prepared by the present invention has a particle size of 80-110 nm and a hydroxyproline content of 1.5-2.7 wt%; when used as a calcium supplement preparation, it has good ability to supplement bone collagen, improves the strength of bone tissue, and will not cause abnormal development of internal organs.

The invention discloses a preparation method of fishbone collagen tripeptide, which comprises the following steps: soaking fishbone dust (30-60 meshes) in a choline-arginine eutectic solvent, stirring, centrifuging or filtering, and collecting supernate; adding pure water with the volume 3-6 times that of the supernatant into the supernatant, standing for more than 24 hours, centrifuging or filtering again, and collecting the supernatant; carrying out tangential filtration treatment by adopting a nanofiltration membrane with the molecular weight cutoff of 200Da, recovering the eutectic solvent in the filtrate through vacuum concentration, adding pure water into the trapped fluid, carrying out repeated nanofiltration treatment to remove solvent residues, and collecting the trapped fluid; and carrying out spray drying on the trapped fluid to obtain a powder product with the collagen tripeptide content of more than 50%. Compared with a traditional enzyme method for preparing fishbone collagen tripeptide, the method disclosed by the invention has the advantages that the extraction rate is high, a deliming step is not needed, the enzyme cost is saved, an extraction solvent is green and can be recycled, and the like.

This application discloses a method and its application for preparing collagen peptides by stepwise enzymatic hydrolysis with dynamic pH control, belonging to the field of protein preparation technology. The method includes raw material pretreatment, stepwise enzymatic hydrolysis with dynamic pH control, and post-treatment to obtain collagen peptide products with a molecular weight distribution of 800-2000 Da. The obtained products can be used to prepare products with bone growth promotion functions. This application employs a combined process of stepwise enzymatic hydrolysis with dynamic pH control, alkali-thermal coupling pretreatment, and 2000 Da retention. The steps work synergistically: alkali-thermal treatment exposes enzyme cleavage sites, dynamic pH control enables efficient enzymatic hydrolysis, and 2000 Da retention of active components results in a significantly higher yield and content of collagen peptides compared to conventional processes. Animal experiments have demonstrated that the collagen peptides have bone growth promotion activity, and the mechanism of action has been revealed at the IGF-1 signaling pathway level.

The invention relates to a preparation method of an immunity-enhancing eel bone peptide composition, and belongs to the technical field of biological extraction. Comprising the following steps: a) pretreatment: sequentially performing low-temperature freeze-drying on eel aggregate, crushing the eel aggregate to 200 meshes or below, degreasing the eel aggregate with ethanol, and performing acid-process deliming to obtain pretreated aggregate; b) three-stage composite enzymatic hydrolysis: performing three-stage composite enzymatic hydrolysis on the pretreated aggregate to obtain enzymatic hydrolysate; c) enzyme deactivation and centrifugation: performing enzyme deactivation on the enzymatic hydrolysate, performing centrifugal separation, and removing insoluble substances to obtain clear liquid; d) ultrafiltration separation: performing ultrafiltration separation on the clear liquid to remove macromolecular impurities so as to obtain a collagen peptide concentrated solution; and e) debitterizing, deodorizing and drying: debitterizing and deodorizing the collagen peptide concentrated solution, and drying to obtain eel bone collagen peptide powder. The fishy smell and bitter taste are effectively removed, and the biological activity, purity and palatability of the product are remarkably improved.

The invention discloses a preparation method of low-molecular-weight takifugu rubripes fishbone collagen peptide. The preparation method comprises the following steps: under a low-temperature condition, naturally unfreezing takifugu rubripes fish bones, washing the fish bones with distilled water, cutting the fish bones into pieces, adding a NaOH solution and stirring to remove impure protein, adding an EDTA-2Na solution and stirring to remove mineral substances, adding an isopropanol aqueous solution and stirring to remove fat, then washing the fish bones with distilled water, drying the fish bones in a drying oven, and crushing the fish bones with a universal crusher to obtain the takifugu rubripes fish bones. Adding water and compound protease, performing enzymolysis in a water bath, inactivating in a boiling water bath, centrifuging, taking supernatant, and performing freeze drying to obtain collagen peptide powder. The preparation raw materials are low in cost and easy to obtain, and the content of crude protein and collagen is high. According to the extraction method, a relatively mild enzyme method is selected for preparation, the influence on the collagen peptide is relatively small, alkaline protease and arenga pinnata plant protease are combined for use, Neutrase enzyme and Alcalase enzyme are combined for use, and a synergistic effect is achieved.

The invention belongs to the technical field of food preparation, and particularly relates to a biological information technology guidance-based compound bovine bone collagen peptide beverage, which is prepared from the following raw materials: bovine bone collagen peptide powder, red date polysaccharide, astragalus polysaccharide, calcium carbonate, chondroitin sulfate and sodium hyaluronate. When the compound bovine bone collagen peptide beverage is drunk, bovine bone contains a large amount of collagen, is rich in type I collagen and is richer in amino acid composition, and a large number of research results show that protein is not completely hydrolyzed into amino acid after being ingested, most of the protein is absorbed in a peptide form, and the protein is absorbed in a peptide form. Therefore, the selected bovine bone collagen peptide powder can be better absorbed by the human body. The red dates have the effects of maintaining beauty, keeping young and resisting aging. In addition, astragalus membranaceus and other traditional Chinese medicine extracts are supplemented in the compound bovine bone collagen peptide beverage, so that the functional characteristics of the beverage are enhanced, and the original bad flavor of animal peptide is improved.

The utility model discloses a low-energy-consumption bone collagen concentration device which comprises a concentration box, a steam recovery system is arranged in the concentration box and used for recovering steam generated by evaporation of bone collagen soup, a compression treatment system is arranged at the bottom of the steam recovery system, and the compression treatment system is used for compressing the bone collagen soup. A compression treatment system is arranged on one side of the concentration box and used for compressing recycled steam, a reheating system is arranged on one side of the concentration box and used for reheating bone collagen soup through the compressed steam, and a plurality of supporting columns are arranged at the bottom of the concentration box. According to the utility model, the energy consumption in the bone collagen concentration process is reduced.

The application discloses a method for determining key amino acid residues of bone collagen peptide promoting osteogenesis activity, belongs to the field of biological medicine and food science, and aims to solve the technical problem that traditional methods are low in efficiency, insufficient in accuracy, and cannot efficiently identify key amino acid residues of bone collagen peptide determining the activity of promoting osteogenesis. The application comprises the following steps: firstly, obtaining target bone collagen peptide and constructing a single-point alanine mutant peptide library thereof; then, sequentially performing conformation and physicochemical property characteristic analysis, binding free energy simulation calculation with specific receptor protein, and cell experiment determination of the activity of promoting osteogenesis on the target peptide and the mutant peptide; finally, screening the key amino acid residues based on multi-level joint determination of characteristic deviation, binding free energy change and activity decline ratio. The application is mainly used for quickly and accurately positioning the core functional site of bone collagen peptide promoting osteogenesis activity, and provides key theory and technical basis for efficient screening, rational design and function strengthening of osteogenic activity peptide.

The invention relates to the technical field of collagen peptide production, in particular to bovine bone collagen peptide production equipment which comprises a shell and a partition plate, a stirring shaft is rotatably connected to the interior of the shell, stirring blades are fixed to the surface of the rotating shaft, the partition plate is arranged in the middle of the shell, and the stirring blades are fixed to the surface of the stirring shaft. The interior of the shell is divided into an upper cavity and a lower cavity by the partition plate, and a communicating groove for communicating the upper cavity with the lower cavity is formed in the partition plate; according to the cattle bone powder mixing device disclosed by the invention, the separation plate is arranged in the shell to separate a production space, and cattle bone powder with high density can be quickly settled due to the gravity effect in a mixing process, so that the cattle bone powder with high density is hydrolyzed in the lower space; and the cattle bone powder with low density in the upper cavity continues to react in the original environment, so that the problems of excessive partial hydrolysis and insufficient partial hydrolysis caused by mixing are avoided, and the quality of a finished product of collagen peptide is favorably stabilized.

The invention discloses a method for preparing a phosphorylated salmon collagen peptide-calcium chelate by taking salmon collagen peptide as a raw material, preparing high-calcium binding active peptide (P-CP) through phosphorylation modification and chelating the high-calcium binding active peptide with calcium ions. The method comprises the following steps: (1) enzymolysis preparation of salmon bone collagen; (2) phosphorylation modification of a peptide fragment mediated by sodium tripolyphosphate; and (3) optimizing a calcium ion chelation process. The chelating rate of the obtained P-CP-Ca calcium reaches 91.21%, and structural characterization proves that a phosphate group (PO4 < 2->) is combined with calcium ions through ionic bonds. Animal experiments show that P-CP-Ca can significantly improve the serum calcium level of a calcium-deficiency mouse (the H group recovers to a normal range), reduce the activity of a bone resorption marker TRAP (Target Related Amplified Protein) (which is 25.4% lower than that of a model group), and improve the bone trabecula microstructure (the bone mineral density is improved by 32.7%). The invention provides a new technology for valuable utilization of fishery by-products and development of functional calcium supplements.

The application discloses a composition capable of effectively inhibiting the growth of Staphylococcus aureus biofilm. The application firstly optimizes the nitrogen source in the culture medium of Staphylococcus epidermidis CCSM0287 strain, fully explores the growth difference of Staphylococcus epidermidis under different culture media and culture conditions and the inhibition effect of the fermentation supernatant on Staphylococcus aureus biofilm, then compounds the fermentation supernatant of Staphylococcus epidermidis CCSM0287 obtained by using the optimized culture medium (the nitrogen source adopts tryptone + soybean peptone + fresh beef bone collagen peptide powder) with the fermentation supernatant of Lactobacillus paracasei S20, can obviously inhibit the growth of Staphylococcus aureus biofilm and destroy the surface structure of the Staphylococcus aureus biofilm, and can be applied in bacteriostatic products.

The invention discloses a composition containing bovine bone collagen as well as a preparation method and application thereof, and belongs to the technical field of collagen application. The composition containing the bovine bone collagen is obtained by chemical crosslinking of bovine bone collagen magnesium chelate, sodium hyaluronate, chondroitin sulfate and bovine bone collagen through EDC / NHS. The composition containing bovine bone collagen is prepared from the following components in parts by weight: 5 to 10 parts of bovine bone collagen magnesium chelate, 2 to 5 parts of sodium hyaluronate, 0.5 to 2 parts of chondroitin sulfate and 20 to 30 parts of bovine bone collagen. The bovine bone collagen magnesium chelate, the sodium hyaluronate, the chondroitin sulfate and the bovine bone collagen in the composition containing the bovine bone collagen prepared by the invention have the characteristics of good biocompatibility, capability of promoting cell proliferation and differentiation into cartilage cells, and suitability for cartilage repair.

The invention belongs to the technical field of protein beverages, and particularly relates to a composite peptide beverage and a preparation process thereof. The active ingredients of the drink comprise calf thymosin, cattle spleen protein peptide, hemoglobin peptide, bone collagen peptide and collagen peptide in a weight ratio of (2-3): 1: 1: 1: 1. The pH value of the system is adjusted to 3.5-4.5, so that each peptide molecule is positively charged under the acidic condition, the electrostatic repulsion and hydration among molecules are enhanced, and bitter groups are effectively masked. The flavoring agent is compounded by DL-malic acid, crystalline fructose, xylitol and sucralose according to the weight ratio of (0.6-1): 1: 0.4: (0.03-0.04), and the sour and sweet taste and flavor coordination are synergistically improved. The preparation method comprises the following steps: dissolving the DL-malic acid in purified water, dissolving the uniformly mixed active components in purified water at 40-60 DEG C, respectively adding other flavoring agents, stabilizing agents and essence, and finally filtering to obtain the product. The problem of bad flavor of the peptide is solved, so that the beverage is appropriate in taste, harmonious in flavor and easy to accept.

The utility model relates to the technical field of biological products, and in particular to a collagen peptide preparation device, comprising two groups of side plates and a casing fixed between the two groups of side plates, a crushing mechanism is installed in the casing, a hopper is fixedly arranged on the outer wall of the top of the casing, a feeding mechanism is arranged at the bottom of the hopper to evenly feed aggregates into the casing, and the feeding mechanism is transmission-connected with the crushing mechanism; the feeding mechanism comprises a transmission assembly transmission-connected with the crushing mechanism, a swing arm is hinged on the transmission assembly, one end of the swing arm is rotationally connected to a bearing frame, the bearing frame is fixedly connected to the outer wall of the casing, a movable hole is opened at the other end of the swing arm, and a fixed block is slidably arranged in the movable hole; the collagen peptide preparation device, by arranging the feeding mechanism, can realize even feeding of the feeding plate each time it moves, avoids the problem of excessive one-time feeding causing a higher operating load of the equipment, and can improve the stability and service life of the equipment.

The invention belongs to the technical field of nutritional health-care food and biological medicine, and particularly relates to a composite peptide composition with bone and cartilage growth promoting ability and a preparation method, application and a product of the composite peptide composition. The composite peptide composition provided by the invention consists of marine fishbone collagen peptide, chondropeptide and eggshell membrane peptide. The composite peptide composition and the composite peptide preparation are good in safety, and the survival rate of chondrocytes and osteoblasts treated by hydrogen peroxide can be remarkably increased. Gene expressions of ACAN, COL1 alpha1, COL2 alpha1, CHST3, HAS3 and OCN in chondrocytes and osteoblasts are up-regulated, and it is prompted that the compound has protection and promotion effects on bone-related cell functions. The composite peptide composition and the composite peptide preparation synchronously act on bone longitudinal growth and cartilage health through multi-target design, provide comprehensive nutrition support for bone and cartilage synergistic development, and have the characteristics of high safety, comprehensive functions and good palatability.

The application provides king salmon bone collagen peptide for promoting longitudinal growth of bone, a preparation method and application, and relates to the technical field of collagen peptides. The preparation method comprises the following steps: (1) treating king salmon bone raw materials by steam explosion to obtain pretreated king salmon bone; then mixing the pretreated king salmon bone with pepsin and performing first enzymolysis under pressure to obtain a first enzymolysis mixture; mixing the first enzymolysis mixture with collagenase and ficin, performing second enzymolysis under pressure, collecting an enzymolysis liquid, and performing ultrafiltration on the enzymolysis liquid to obtain collagen peptide. In the application, pepsin is used for first enzymolysis under pressure, then collagenase and ficin are used for compounding, the synergistic effect of the three kinds of proteases makes the collagen be fully enzymolyzed, the diversity of the cleavage site is improved, the molecular weight of the polypeptide is reduced, the activity of the polypeptide is improved, the longitudinal growth of the bone is promoted, and the bone density is improved.

The present invention discloses a glucosamine chondroitin composition and a preparation method thereof, belonging to the field of health care products. The composition comprises a turmeric extract, a drynaria extract, an eucommia extract, a rehmannia extract, vitamin K, bovine collagen peptides, chondroitin sulfate, glucosamine hydrochloride, and lactic acid calcium. The present invention achieves an excellent bone density-enhancing effect by compounding the turmeric extract, the drynaria extract, the eucommia extract, the rehmannia extract, vitamin K, bovine collagen peptides, chondroitin sulfate, glucosamine hydrochloride, and lactic acid calcium.

The present invention provides a high-performance conical columnar bone-level implant, which includes: an implant structure, a locking structure located below the implant structure, and an abutment structure. The application uses the implant structure for installation, which can be closely fitted with the alveolar socket of the alveolar bone to achieve stable implantation. The tip formed by setting the cutting groove can improve the implantation stability, making it have a certain self-tapping property in the horizontal direction. And a lock is pre-implanted, and the cooperation between the implant and the lock is used to achieve limit, the lock is clamped with the alveolar bone, and a stable connection with the implant is achieved. Stable limit can be achieved by inserting a rod into the interior of the alveolar bone. While installing, the internal space of the implant is squeezed, so that the pre-filled bone collagen is squeezed into the gap between the implant and the alveolar bone to complete the filling, which not only improves the implantation stability of the implant, but also can achieve the function of rapid bone bonding, shorten the recovery time of tooth implantation, and improve the service life after the implant is implanted.

The invention provides a high-purity bovine bone collagen peptide gel filtration chromatography purification method, and belongs to the technical field of bioactive peptide purification. The method comprises the following five core steps: pretreatment, gel column preparation, dynamic equilibrium, sample loading and elution, and post-treatment: firstly, carrying out ultrafiltration pretreatment on a bovine bone collagen peptide crude extract, then filling a chromatographic column with a specific diameter-height ratio by using cross-linked sephadex, carrying out dynamic equilibrium by using an accurately adapted equilibrium liquid after column efficiency verification, and finally, carrying out dynamic equilibrium by using a chromatographic column with a specific diameter-height ratio. The sample loading amount is dynamically matched through a formula, a gradient isothermal mode is adopted in the elution process, and finally a high-purity product is obtained through freeze drying. Through multi-step parameter collaborative optimization and precise regulation and control of a self-created formula, the problems of low purification efficiency, poor product purity and wide molecular weight distribution in the prior art are solved, the purity of the obtained bovine bone collagen peptide is greater than or equal to 98%, the proportion of peptide fragments with molecular weight distribution of 500-3000Da and 1000-2000Da is greater than or equal to 70%, the heavy metal content and the colony count both meet high-standard requirements, the yield is stabilized at 52% or above, and the method is suitable for industrial production. Good industrial application prospects are realized.