79 results about "Growth hormone" patented technology

The invention discloses heavy metal resistant bacillus velezensis as well as a microbial remediation agent and application thereof. Bacillus velezensis DBS01 is screened from heavy metal contaminated soil in Dabaoshan mining areas in Shaoguan City, Guangdong province, is preserved in China Center for Type Culture Collection (CCTCC), has a preservation number of CCTCC M 20252228, not only has resistance to various heavy metals, but also can metabolize and secrete metabolites such as organic acid, cellulase and growth hormone in different growth stages, and can be used for preventing and treating various heavy metals. Multiple functions of curing heavy metals, dissolving phosphorus and potassium, promoting plant growth and the like are realized. According to the method, bacillus velezensis DBS01 is inoculated into a culture medium containing straw, calcium superphosphate, agar and sodium alginate are added, and a mixture containing the bacillus velezensis DBS01 is freeze-dried, so that the heavy metal contaminated soil in the mining area can be efficiently repaired in an environment-friendly manner at low cost, and the ecological function recovery of the soil is effectively promoted.

The present invention provides for the immediate or chronic administration of certain potassium ATP (K ATP ) channel openers, optionally in combination with growth hormone, to subjects to achieve novel pharmacodynamic, pharmacokinetic, therapeutic, physiological, metabolic, and compositional outcomes in the treatment of diseases or conditions involving K ATP channels. Also provided are pharmaceutical formulations, methods of administration, and methods of dosing K ATP channel openers to achieve these outcomes and to reduce the incidence of adverse effects in treated individuals. Also provided are methods of co-administering K ATP channel openers with other drugs, e.g., in combination with growth hormone, to treat diseases in humans and animals, e.g., Prader-Willi Syndrome (PWS), Smith-Magenis Syndrome (SMS), etc.

The application discloses application of SbWOX gene and SbBBM gene in corn breeding and a corn genetic transformation method, and relates to a bioengineering technology.The application provides a nucleic acid molecule with co-expression of SbWOX protein gene and SbBBM protein gene, and corresponding gene co-expression vectors and microbial transformants.The application also provides application of the co-expression of the SbWOX gene and the SbBBM gene in the corn genetic transformation method.The application obtains corresponding genes and mutant proteins based on the SbWOX gene and the SbBBM gene of sorghum, the two genes are co-expressed in the corn genetic transformation process, even without adding plant growth hormones in the antibiotic screening medium in the tissue culture process, the plant genetic transformation process can be normally completed, and the transformation efficiency and the regeneration efficiency of the corn can be remarkably improved, the transformation rate reaches 10.42%, and the differentiation rate reaches 32.89%.

The invention provides Rhodococcus qingshengii FJ5 capable of promoting tobacco growth and preventing and treating bacterial wilt and application of the Rhodococcus qingshengii FJ5, and belongs to the technical field of microorganisms. The invention provides a rhodococcus FJ5 strain, and the collection number of the rhodococcus FJ5 strain is CGMCC No.32631. The FJ5 strain is wide in application range, and can well grow under the condition that the temperature is 16-34 DEG C and the pH value is 5.0-8.0. The FJ5 strain promotes early growth of tobaccos and enhances stress resistance by relieving acid-aluminum stress, improving defensive enzyme activity of the tobaccos and improving secretion of growth hormones of the tobaccos and the carbon and nitrogen metabolism level of the tobaccos, and meanwhile, improves fertility of acid soil and prevents and treats bacterial wilt of the tobaccos. The Rhodococcus FJ5 provided by the invention has important development value in high-quality development of tobacco under the condition of rice-tobacco rotation acidified soil, and also has important significance in improvement of stress resistance of plants and biological control of root and stem diseases.

The present invention describes for the first time a method for preventing and / or reversing the phenomenon of premature senescence of skin cells and complex chronic wounds, characterized in that an effective amount of the growth hormone secretagogue peptide GHRP6 is applied. Also object of the present invention is a method for treating cases of complex chronic wounds that have developed primary or secondary refractoriness caused by the development of a cellular senescence process, transforming them from refractory wounds to responsive wounds in the presence of proliferative agents with which they unexpectedly manage to reverse and restart the healing process. Complex chronic wounds include cases of diabetic foot ulcers, venous ulcers, pressure ulcers, radiation ulcers, and other types of chronic wounds that are characterized by refractoriness to all types of treatment. These cases currently have no effective treatments.

The present invention provides processes for producing a bioengineered lung (BEL) from an acellular lung matrix that has been treated with growth hormones, seeded with primary lung cells, and cultured in a bioreactor. Also provided are BELs and methods of transplanting the BEL into a subject in need of a lung transplant, and methods for using BELs for the study of the lung microbiome and its role in lung development and remodeling.

Disclosed are a compound shown in formula I and racemate, stereoisomer, tautomer, isotopically labeled, nitroxide, solvate, polymorph, metabolite, ester, prodrug or pharmaceutically acceptable salt thereof, and a pharmaceutical composition comprising the same, a preparation method thereof, and a use thereof as a GHSR agonist in the preparation of a drug for the diagnosis, prevention and / or treatment of growth hormone-dependent diseases, the structure of formula I is as follows:

A method for producing a metabolism-activated liver organ, comprising a step for culturing primary hepatocytes or hepatocyte-like cells in a differential medium to obtain a metabolism-activated liver organ, the differential medium is a medium containing a glucocorticoid receptor agonist and one or two selected from the group consisting of a growth hormone receptor agonist and a prolactin receptor agonist.

The invention relates to the technical field of poultry breeding, and particularly discloses an antibiotic-free breeding method for improving the quality of old yard black chickens based on compound plant alternate feeding, and in the growth process of chickens, plant complementary food and basic ration without antibiotics and growth promoting hormone are added for mixed feeding; the adding method of the plant complementary food comprises the following steps: (1) forming a three-day alternating unit according to the sequence of feeding fresh Chinese chives on the first day, feeding fresh wild wormwood on the second day and feeding fresh wild banana trees on the third day, and circularly repeating the mode until the chickens are slaughtered; (2) the adding proportion of the plant complementary food is increased along with the day age of the chickens, and the adding proportion is the mass percentage of the total feeding weight of the day. According to the antibiotic-free breeding method, the specific composite plant feed is adopted for alternate feeding, and the meat flavor, the nutritional value and the health level of the chickens are remarkably improved through functional complementation and time sequence collaboration of plant active ingredients.

The application discloses a transdermal patch special for children with dwarfism, and relates to the technical field of medical treatment, which comprises a patch main body and a needle cylinder, and a fixed plate is fixedly connected to the inside of the patch main body, and the top and the bottom of the fixed plate are penetrated by the needle cylinder; the top of the needle cylinder is provided with a pressing plate, the bottom of the pressing plate is provided with a stand column on the two sides of the needle cylinder, the bottom of the two stand columns is arranged in the inside of the fixed plate and is slidably connected with the fixed plate; the two stand columns are both sleeved with a spring one; the top of the pressing plate is provided with a clamping groove, the bottom of the clamping groove is communicated with the needle cylinder, and a clamping seat is arranged in the clamping groove; the top of the pressing plate is provided with a fence; the two sides of the pressing plate are both provided with a connecting rod, one end of the connecting rod is connected with the pressing plate, and the other end penetrates through the top of the patch main body; the two sides of the patch main body close to the bottom are both provided with a sticking layer; and the two sides of the inside of the patch main body are both provided with a negative pressure sponge layer. The burden of the children with dwarfism is relieved, growth hormone injection treatment is facilitated, and the skin redness and swelling caused after growth hormone injection can be effectively prevented and treated.

The present invention relates to a customizable injectable solution comprising Human Growth Hormone (HGH), Insulin-like Growth Factor 1 (IGF-1), or a growth hormone secretagogue such as sermorelin or ipamorelin, combined with a blend of nutrients including amino acids, vitamins, electrolytes, functional mushroom extracts, and optionally mushroom mycelium. The solution is delivered via a dual-chamber syringe to preserve component stability, with mixing occurring immediately before administration. The formulation is suitable for subcutaneous, intramuscular, or intravenous injection and may be tailored for muscle growth, cognitive performance, immune support, or neuroregeneration. The invention also contemplates injectable formulations of functional mushrooms alone, as well as alternative delivery methods such as sublingual troches containing GH-inducing peptides, functional mushrooms, and ketamine. This system provides a versatile, bioavailable, and clinically adaptable approach to personalized health optimization.

The present disclosure relates to pharmaceutical compositions comprising a compound of Formula (I) or a pharmaceutically acceptable salt thereof, and their use in treating growth hormone deficiency or a condition associated with an abnormal reduction in growth hormone (GH) secretion in a subject that has adequate GH secretion potential.

The invention discloses a villous amomum fruit seedling tissue culture rapid propagation method, which is characterized in that villous amomum fruit seeds are used as a material, MS (Murashige and Skoog) is used as a basic culture medium, growth hormones with different proportions are added, adventitious buds are induced to proliferate and propagate, a rapid propagation technical system of villous amomum fruit is established, and after kinetin is added into the induction and proliferation culture medium, the germination speed is high, the germination rate is high, the buds are robust, and the proliferation coefficient is high; after the indoleacetic acid is added into the rooting culture medium, the rooting is fast, the rooting rate is high, the roots are many, the stems are thick, the leaf emergence is fast, the leaves are fleshy and good in growth vigor, and the transplanting survival rate is greatly improved. According to the method, the problems that the fructus amomi seeds are not easy to store and the propagation of the fructus amomi is influenced are solved, the problems that the division propagation rate of the fructus amomi is low and large-scale planting is limited are solved, large-scale propagation of the fructus amomi can be carried out in a short time through the method, and the production and planting requirements are met.

The invention relates to a deep learning and logic correction segmentation combined bone age assessment method, which aims at the problems of high joint extraction false drop rate, difficulty in epiphysis maturity grade classification and the like, through a logic correction segmentation mechanism of confidence ranking, a semi-supervised pseudo-label generation strategy and a global attention enhancement module based on normalization, the bone age is assessed. And the accuracy and clinical robustness of bone age evaluation are remarkably improved. The application scenarios of the method include but are not limited to the precise medical fields of children endocrine dysplasia screening, bone age lag or advance pathological diagnosis, orthopedic treatment scheme making, growth hormone intervention curative effect evaluation and the like.

The application discloses a method for preparing GHR humanized animal cells by using a CRISPR system, which comprises the following steps: designing and synthesizing sgRNA; mixing the sgRNA with a Cas9 protein to form a CRISPR-Cas9 complex; constructing donor DNA containing coding sequences of a human GHR extracellular region and a rat GHR intracellular region; injecting the CRISPR-Cas9 complex and the donor DNA into the cytoplasm or nucleus of a rat fertilized ovum to knockout the coding sequence of the extracellular region of the rat-derived GHR gene and simultaneously insert the coding sequence of the extracellular region of the human-derived GHR gene into the genome, so that the GHR humanized animal cells are obtained. The application further discloses a method for constructing a GHR humanized rat model by using the CRISPR system, and application of the GHR humanized animal cells and the GHR humanized rat model obtained by the above method in drug screening, drug development or efficacy evaluation. The application can more accurately simulate the interaction between the human GHR and growth hormone and other ligands, improves the accuracy and reliability of research, and has the advantages of simple gene editing operation, high editing efficiency, strong specificity, low off-target effect and wide application range.

The invention discloses a sterile production method of a fresh dendrobium officinale protocorm product, which comprises the following steps: taking dendrobium officinale seeds from capsules of dendrobium officinale, repeatedly washing the capsules with tap water until the capsules are free of foam, sterilizing, cutting the capsules open, and drying to obtain the dendrobium officinale protocorm product. Uniformly spreading the seeds on an M2 solid culture medium, keeping the illumination condition of 500Lx and the temperature of 25 + / -1 DEG C, and waiting for the seedlings to grow for 60-90 days; wherein the M2 solid culture medium comprises 1 / 2 MS macroelements without exogenous hormones, 35% of banana juice, 35% of composite carbon source and 2% of agar powder. The method has the beneficial effects that a technical route map for mass production by infinitely stacking sterilization and ventilation culture of the small glass containers of 10 liters and getting rid of the constraint of a shaking table is provided. The technical key point is to screen out that the growth rate which can reach 8.4 mg / g.d without depending on plant growth hormone (which is a rigid biological index).

The invention belongs to the technical field of agriculture, and particularly relates to a degerming and seedling formation solution, a preparation method, an application method and a method for promoting seed germination and seedling formation. The degerming and seedling formation solution provided by the invention comprises gibberellic acid, indoleacetic acid, naphthylacetic acid, coptis chinensis and carbendazim, and the combination of coptis chinensis extract, carbendazim and growth hormone (gibberellic acid, indoleacetic acid and naphthylacetic acid) in a specific proportion has a synergistic inhibition effect on alternaria alternata and has a promotion effect on seed germination and seedling formation. The invention provides the preparation method of the degerming and seedling forming solution, and the application is simple. According to the application method of the degerming and seedling forming solution, the operation steps are simple, convenient and efficient, and the seedling forming efficiency is remarkably improved. The result of the embodiment shows that the germination rate of the Sinojackia xylocarpa seeds reaches 100% after the optimal treatment is performed for 15 days, and the survival rate of the seed seedlings reaches 96.67% after the optimal treatment is performed for 30 days.

The invention belongs to the technical field of pharmaceutical preparations, and particularly relates to an oral growth hormone pharmaceutical composition and a preparation method thereof. The composition comprises at least one growth hormone with an effective dose, at least one absorption enhancer, at least one pH regulator and at least one excipient. The pharmaceutical composition disclosed by the invention effectively promotes the absorption of the oral growth hormone drug, and further improves the bioavailability of the growth hormone drug.

The invention relates to a preparation method and application of accurately-penetrating camellia saponin A reverse micelle oil. The camellia saponin A reverse micelle oil is prepared from the following components in percentage by mass: 0.5 to 1.5 percent of camellia saponin A; the glass color factor is 1-2%; 36 to 40% of a nonionic surfactant; 8-12% of short chain alcohol; 6-11% of long-chain alcohol; and 40-44% of grease. The invention accidentally finds that the compatibility of the camellia saponin A and the reverse micelle system can be improved by introducing the glassine, the loading capacity of the camellia saponin A in the reverse micelle oil is increased to 1.5% from less than 0.5%, and meanwhile, the reverse micelle oil is clear and transparent in appearance and has good stability. The camellia saponin A reverse micelle oil can be added into a pure oil system, the application field of the camellia saponin A is expanded, and the camellia saponin A reverse micelle oil has important application value in the field of cosmetics.

The invention relates to two variable spliceosomes of a cynoglossus semilaevis adcyap1b gene and application of the two variable spliceosomes, and belongs to the technical field of functional gene screening and application in the aquatic biotechnology, the two variable spliceosomes are adcyap1b-tv1 and adcyap1b-tv2, coding region CDS sequences of the adcyap1b-tv1 and the adcyap1b-tv2 are SEQ ID NO: 1 and SEQ ID NO: 2, and protein sequences of the adcyap1b-tv2 are SEQ ID NO: 3 and SEQ ID NO: 4. The two variable spliceosomes of the adcyap1b gene are applied to regulation and control of hormone secretion of the pituitary of the cynoglossus semilaevis. By adding the recombinant protein of which the two variable spliceosomes are adcyap1b-tv1 and adcyap1b-tv2 into pituitary cells, the expression of a growth hormone gene gh, a prolactin gene prl, a growth lactin gene sl, a proogonin gene pomc and an adrenocorticotropic hormone release hormone gene crh of the cynoglossus semilaevis is regulated and controlled, so that the growth and development of the cynoglossus semilaevis are influenced.

This invention discloses a gene ThERF053 related to lateral branch development in *Taxus chinensis*, its expressed protein, and its applications, belonging to the field of plant genetic engineering technology. This application cloned a *Taxus chinensis* ThERF053 gene, the nucleotide sequence of which is shown in SEQ ID NO:1, and the amino acid sequence of the expressed protein is shown in SEQ ID NO:2. By transferring the ThERF053 gene into the model plant *Populus*, transgenic poplars overexpressing ThERF053 showed a 980-5100-fold increase in ThERF053 expression, while the content of the growth hormone IAA in the transgenic poplar plants was lower than that in the control plants. After 30 days of growth, a significant increase in lateral branches was observed in the transgenic *Populus chinensis*. These experimental results indicate that ThERF053 is a key gene positively regulating lateral branch development in *Taxus chinensis*, and has important application value in the molecular breeding of *Taxus chinensis* and related tree species.

The application discloses a kind of auxin response SAUR gene family protein genes and purposes thereof, and belongs to the technical field of genetic engineering.The application first finds that the expression pattern of auxin response SAUR gene family protein gene HvSAUR36 is significantly positively correlated with the accumulation of valine, leucine and isoleucine.The application constructs HvSAUR36 overexpression vector and transforms tobacco, and detection shows that the content of valine, leucine and isoleucine in the leaves of transgenic lines is significantly higher than that of wild type.It is proved that the auxin response SAUR gene family protein encoded by HvSAUR36 plays a key catalytic role in the biosynthesis pathway of plant branched-chain amino acids, and provides an important target gene for crop quality improvement.The gene, its recombinant vector, recombinant bacteria and transgenic plants all have good application prospects.

The invention discloses a strain of nitrogen-fixing and acid-producing bacillus strain Paenibacillus sabinae N56, which not only has relatively strong free living nitrogen fixing capacity, but also has the capacity of secreting organic acids (oxalic acid and formic acid), especially has the function of activating inorganic phosphorus (P) in farmland soil, and can be used for preparing a nitrogen-fixing and acid-producing bacillus strain N56, and the nitrogen-fixing and acid-producing bacillus strain N56 can be used for preparing a nitrogen-fixing and acid-producing bacillus strain N56. Meanwhile, growth hormone indoleacetic acid can be secreted, so that the growth of crops is promoted, and the saline-alkaline tolerance is realized. Meanwhile, culture amplification of the nitrogen-fixing paenibacillus N56 is optimized, the problem that the yield of spores of the N56 is low is solved, the number of the spores is increased to 2 billion cfu / ml from 2 billion cfu / ml, the yield of the spores is increased by 10 times, the application potential of the N56 is enhanced, and the production cost can be effectively reduced.

The invention discloses an auxin response SAUR gene family protein gene and application thereof, and belongs to the technical field of gene engineering. The invention discovers that the expression mode of the auxin response SAUR gene family protein gene HvSAUR36 is obviously positively correlated with the accumulation of valine, leucine and isoleucine for the first time. According to the invention, an HvSAUR36 overexpression vector is constructed and is used for transforming tobacco, and detection shows that the valine content, the leucine content and the isoleucine content in leaves of a transgenic line are obviously improved compared with those of a wild type. The HvSAUR36-coded auxin response SAUR gene family protein is proved to play a key catalytic role in a plant branched chain amino acid biosynthetic pathway, an important target gene is provided for crop quality improvement, and the gene and a recombinant vector, a recombinant bacterium and a transgenic plant thereof have good application prospects.

The invention discloses a method for regulating and controlling propagation of populus euphratica and stability of rhizosphere microorganisms through branched seepage rotation irrigation, and relates to the technical field of ecological restoration. According to the method, by differentiating branched seepage rotation irrigation modes, precisely matching habitat requirements of sexual and clone propagation populus euphratica seedlings, and adopting a long-interval and gradually-water-increasing irrigation mode for the clone propagation seedlings, the propagation of populus euphratica and the stability of rhizosphere microorganisms are regulated and controlled; according to the method, soil salt leaching and nitrogen and phosphorus accumulation can be promoted, functional florae such as rhizosphere actinomycetes and thick-wall bacteria are activated, complex organic matter is degraded by secreting extracellular enzyme, the phosphorus dissolving capacity is enhanced, the nutrient obtaining efficiency of seedlings in a medium-high-salt environment is improved, a short-interval and high-water-quantity mode is adopted for sexual propagation seedlings, a low-salt and high-humidity habitat is created, and the survival rate of the seedlings is increased. Growth-promoting florae such as pseudomonas are enriched, and by secreting growth hormones and antagonistic substances, the planting capacity of the seedlings in a humid environment is enhanced, so that directional regulation and control of the two breeding modes are realized.

This invention provides a method for promoting tobacco growth and preventing bacterial wilt. Rhodococcus qingshengii This invention relates to the field of microbial technology, specifically to Rhodococcus faecalis strain FJ5, with the preservation number CGMCC No. 32631. The FJ5 strain exhibits a wide adaptability range, growing well within a temperature range of 16–34°C and a pH range of 5.0–8.0. The FJ5 strain promotes early growth and enhances stress resistance in tobacco by mitigating aluminum stress, increasing the activity of tobacco defense enzymes, improving tobacco growth hormone secretion and carbon and nitrogen metabolism, while simultaneously improving acidic soil fertility and controlling tobacco bacterial wilt. The Rhodococcus faecalis FJ5 provided by this invention has significant development value for high-quality tobacco development under acidified soil conditions in rice-tobacco rotation, and is also important for improving plant stress resistance and the biological control of root and stem diseases.

The invention belongs to the technical field of soil remediation, and particularly relates to a method for remedying acetochlor-contaminated soil by compounding growth-promoting bacteria and soybeans. The efficient growth promoting function of the Klebsiella sp.D5A strain is utilized, the Klebsiella sp.D5A strain secretes growth hormone to promote soybean growth, and the adaptive capacity of soybeans to the acetochlor polluted environment is enhanced; after being combined with an acetochlor-tolerant soybean variety, the soybean repairing agent can realize synchronous improvement of the repairing efficiency and the soybean yield through a'growth promotion-stress resistance 'dual synergistic mechanism and by means of the acetochlor degradation capability of the tolerant soybean variety, and solves the problems of single microbial function and disjunction of repairing and production in the prior art.

The invention belongs to the field of mixed fertilizers, particularly relates to a mixed fertilizer for herbal coffee, and provides the following scheme aiming at the problems that most of existing fertilizers for herbal coffee are relatively single and are not beneficial to growth. The fertilizer comprises the following raw materials in percentage by weight: 40-46% of monopotassium phosphate, 35-41% of ammonium dihydrogen phosphate, 1-5% of calcium chloride, 0.5-1% of zinc sulfate, 1-3% of ferric sulfate, 1-2.1% of borax and 1-1.9% of auxin, and has the advantages of balanced nutrition, accurate root promotion, improvement of yield and quality, substantial enhancement of stress resistance of plants, guarantee of stable yield, scientific formula, synergistic interaction of all the components, stable physical properties, and no pollution to the environment. Application is convenient, and absorption efficiency is high.

The invention discloses a composition containing gamma-aminobutyric acid for promoting growth and development of children and a preparation method thereof, the composition comprises gamma-aminobutyric acid and two newly designed modified compounds N-(4-trifluoromethylbenzyl)-gamma-aminobutyric acid amide and a gamma-aminobutyric acid-zinc complex, and functional components such as animal bifidobacterium subsp.lactis, hydrolyzed yolk powder, casein phosphopeptides, minerals and theanine are supplemented. The preparation process comprises the following steps: respectively dissolving and homogenizing the components, mixing step by step in a protective atmosphere, and finally degassing, filling and performing low-temperature irradiation sterilization. The two modified compounds are prepared through activation amidation and complex reaction respectively, and the blood-brain barrier transmittance, stability and bioavailability of original components are remarkably improved. The composition can effectively promote secretion of growth hormones, improve bone growth and improve sleep quality through a multi-target synergistic effect, and is suitable for promoting growth and development of children.

The present invention relates to a customizable injectable solution comprising Human Growth Hormone (HGH), Insulin-like Growth Factor 1 (IGF-1), or a growth hormone secretagogue such as sermorelin or ipamorelin, combined with a blend of nutrients including amino acids, vitamins, electrolytes, functional mushroom extracts, and optionally mushroom mycelium. The solution is delivered via a dual-chamber syringe to preserve component stability, with mixing occurring immediately before administration. The formulation is suitable for subcutaneous, intramuscular, or intravenous injection and may be tailored for muscle growth, cognitive performance, immune support, or neuroregeneration. The invention also contemplates injectable formulations of functional mushrooms alone, as well as alternative delivery methods such as sublingual troches containing GH-inducing peptides, functional mushrooms, and ketamine. This system provides a versatile, bioavailable, and clinically adaptable approach to personalized health optimization.