12 results about "Growth hormone" patented technology

Disclosed are a compound shown in formula I and racemate, stereoisomer, tautomer, isotopically labeled, nitroxide, solvate, polymorph, metabolite, ester, prodrug or pharmaceutically acceptable salt thereof, and a pharmaceutical composition comprising the same, a preparation method thereof, and a use thereof as a GHSR agonist in the preparation of a drug for the diagnosis, prevention and / or treatment of growth hormone-dependent diseases, the structure of formula I is as follows:

The application discloses a method for preparing GHR humanized animal cells by using a CRISPR system, which comprises the following steps: designing and synthesizing sgRNA; mixing the sgRNA with a Cas9 protein to form a CRISPR-Cas9 complex; constructing donor DNA containing coding sequences of a human GHR extracellular region and a rat GHR intracellular region; injecting the CRISPR-Cas9 complex and the donor DNA into the cytoplasm or nucleus of a rat fertilized ovum to knockout the coding sequence of the extracellular region of the rat-derived GHR gene and simultaneously insert the coding sequence of the extracellular region of the human-derived GHR gene into the genome, so that the GHR humanized animal cells are obtained. The application further discloses a method for constructing a GHR humanized rat model by using the CRISPR system, and application of the GHR humanized animal cells and the GHR humanized rat model obtained by the above method in drug screening, drug development or efficacy evaluation. The application can more accurately simulate the interaction between the human GHR and growth hormone and other ligands, improves the accuracy and reliability of research, and has the advantages of simple gene editing operation, high editing efficiency, strong specificity, low off-target effect and wide application range.

This invention provides a method for promoting tobacco growth and preventing bacterial wilt. Rhodococcus qingshengii This invention relates to the field of microbial technology, specifically to Rhodococcus faecalis strain FJ5, with the preservation number CGMCC No. 32631. The FJ5 strain exhibits a wide adaptability range, growing well within a temperature range of 16–34°C and a pH range of 5.0–8.0. The FJ5 strain promotes early growth and enhances stress resistance in tobacco by mitigating aluminum stress, increasing the activity of tobacco defense enzymes, improving tobacco growth hormone secretion and carbon and nitrogen metabolism, while simultaneously improving acidic soil fertility and controlling tobacco bacterial wilt. The Rhodococcus faecalis FJ5 provided by this invention has significant development value for high-quality tobacco development under acidified soil conditions in rice-tobacco rotation, and is also important for improving plant stress resistance and the biological control of root and stem diseases.

The application provides a Klebsiella variicola M15C3 and application thereof, and belongs to the technical field of agricultural microorganisms. The application provides the Klebsiella variicola M15C3, and the preservation number of the Klebsiella variicola M15C3 is CGMCC NO.26628. The Klebsiella variicola M15C3 provided by the application has the lignin degradation characteristics, can promote the decomposition of straw returned to the field, and improves the utilization efficiency of straw returned to the field; the Klebsiella variicola M15C3 has the phosphorus solubilizing ability, can improve the content of available phosphorus in soil, and improves the soil environment; the Klebsiella variicola M15C3 can secrete growth hormones, promotes the seed germination of crops, promotes the growth of crops, and realizes the yield increase of crops. The Klebsiella variicola M15C3 provided by the application has a positive effect in accelerating the transformation of straw returned to the field, improving the soil fertility and promoting the growth of crops.

The application belongs to the technical field of microbial engineering, and particularly relates to a strain of algidbacter algidus and application thereof in improving salt tolerance of crops. Microbacterium algeriense The algidbacter algidus is preserved in the China Center for Type Culture Collection, and the preservation number is CCTCC NO: M 20242839. The strain has the dual promoting growth characteristics of high production of iron carrier and secretion of plant growth hormone, and can significantly relieve the inhibition of salt stress on seed germination, seedling emergence and growth of crops. The algidbacter algidus and the microbial inoculant provided by the application have wide application prospects in promoting seed germination, seedling emergence and growth of crops in saline-alkali land.

This invention discloses a *Pseudomonas* strain that controls citrus canker and promotes plant growth, along with its applications. *Pseudomonas* sp. CR1201 was deposited on January 17, 2024, at the Guangdong Provincial Microbial Culture Collection Center (GDMCC), with accession number GDMCC No.: 64290. The *Pseudomonas* strain disclosed in this invention is a new species in this genus, capable of effectively antagonizing *Xcc*, the causal agent of citrus canker, and promoting plant growth by producing siderophores and the plant growth hormone IAA. Therefore, *Pseudomonas* CR1201 disclosed in this invention can be used to prepare microbial preparations for controlling citrus canker, and also has significant application potential in microbial agents and biofertilizers for promoting plant growth and development.

The application relates to a root system distribution imaging contrast medium, a preparation method, an in-situ imaging system and a method, belongs to the field of ecological restoration and protection, and is characterized in that the contrast medium is mixed by a composite growth hormone, a dissolving agent, a tracer and a solvent; the in-situ imaging system comprises emitting devices for emitting neutron beams to plant root systems, receiving devices for receiving the neutron beams, imaging devices for converting the received neutron beams into images, and two drilling pipes for drilling, each of the drilling pipes comprises a hollow pipe with a top opening and a drill bit arranged at the bottom of the hollow pipe, a positioning structure is arranged in the hollow pipe, and the positioning structure comprises a positioning chuck and a guide pipe arranged on the upper surface of the positioning chuck. The application can effectively adapt to the topography of mountainous areas, eliminate the environmental influence of mountainous areas, and improve the three-dimensional imaging precision and efficiency of the neutron radiation in-situ testing technology for the root system distribution of plants in mountainous areas.

This invention provides a deep-sea yeast strain TDC01 from Antarctica and its application in loosening soil, nourishing the soil, and promoting root growth. It belongs to the field of agricultural microbial technology. The preservation number of the yeast strain TDC01 is CGMCC No. 39165. The yeast strain TDC01 originates from deep-sea sediments below 4000 meters in the Southern Ocean of Antarctica and possesses multiple activities including phosphorus and potassium solubilization, production of growth hormones, production of cellulase-degrading enzymes, and production of pectinase. It can significantly improve soil compaction, activate soil nutrients, and promote crop root growth, thereby achieving the effects of loosening soil, nourishing the soil, and promoting root growth. It has great application prospects in the fields of soil improvement and green bio-fertilizers.

The present application relates to the field of tissue culture, in particular to a culture medium combination for culturing Hibiscus syriacus and application thereof in rapid propagation of Hibiscus syriacus. The culture medium combination for culturing Hibiscus syriacus comprises a differentiation medium, an adventitious bud elongation subculture medium and a strong root culture medium, wherein the differentiation medium comprises differentiation medium I and differentiation medium II, the present application uses part of the differentiation medium in different stages, can fully meet the application required by growth of callus in different stages, improves callus differentiation rate and average bud number, and improves propagation efficiency; the adventitious bud elongation subculture medium is optimized from the perspective of different basic culture media and different growth hormones, finally guarantees to obtain the best adventitious bud elongation subculture medium, increases effective bud number, guarantees survival rate, finally selects a specific rooting culture medium, and increases rooting rate. It can be seen that the culture medium combination can improve propagation rate of Hibiscus syriacus and guarantee survival rate of Hibiscus syriacus.

A method for producing a metabolically activated liver organoid, the method including culturing primary hepatocytes or hepatocyte-like cells in a differentiation medium to obtain a metabolically activated liver organoid, in which the differentiation medium is a medium including one or two selected from the group consisting of a growth hormone receptor agonist and a prolactin receptor agonist, and a glucocorticoid receptor agonist.

The application discloses a probiotic microecological preparation for promoting height growth of children and application thereof, and is characterized by comprising at least one of a fermented Lactobacillus mui ZGG35 strain with a preservation number of CGMCC No. 37997 and a plant Lactobacillus lactis ZGG180 strain with a preservation number of CGMCC No. 37998. The fermented Lactobacillus mui ZGG35 and the plant Lactobacillus lactis ZGG180 are inoculated into a fermentation culture medium at a proportion of 15% in volume ratio to obtain lactic acid bacteria culture, and the ZGG35 bacterial liquid and / or the ZGG180 bacterial liquid is added into a freeze-drying agent to prepare the probiotic microecological preparation. The probiotic microecological preparation has the advantages that the levels of growth hormone, insulin-like growth factor-1, insulin-like growth factor binding protein, alkaline phosphatase, osteocalcin, type I procollagen amino-terminal propeptide, insulin, leptin and vitamin D in serum can be improved, and the levels of IL-6 and CTX-I can be reduced.