Heavy metal resistance plant growth-promoting bacteria preparation and applying method thereof

A technology of plant growth-promoting bacteria and heavy metals, which is applied in the direction of plant growth regulators, plant growth regulators, botany equipment and methods, etc. It can solve the problems of low absorption and accumulation of heavy metals, slow growth, and small biomass of repaired plants. Problems, to achieve the effect of promoting plant growth and development, promoting absorption and utilization, and improving the ability to resist diseases and insect pests

Inactive Publication Date: 2010-03-17
AGRO ENVIRONMENTAL PROTECTION INST OF MIN OF AGRI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Compared with traditional heavy metal pollution remediation methods, phytoremediation has the advantages of environmental friendliness, no secondary pollution, and low remediation cost, which makes phytoremediation a hot spot in the research of heavy metal pollution remediation, but phytoremediation also has the potential to restore plant biomass. Bottleneck problems such as small size, slow growth, and less absorption and accumulation of heavy metals by plants per unit area

Method used

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  • Heavy metal resistance plant growth-promoting bacteria preparation and applying method thereof
  • Heavy metal resistance plant growth-promoting bacteria preparation and applying method thereof
  • Heavy metal resistance plant growth-promoting bacteria preparation and applying method thereof

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Effect test

Embodiment 1

[0020] Embodiment 1: Isolation and identification of the central bacterial strain used in the present invention:

[0021] The bacterial strain used in the present invention is obtained from the separation and screening of heavy metal compound polluted soil in Guangdong. It has resistance to multiple heavy metals (lead, cadmium, copper, zinc) and can produce plant growth hormone, fix nitrogen, dissolve insoluble inorganic phosphorus, and activate insoluble inorganic phosphorus. Heavy metal salts (CdCO 3 ) strain D54, through 16S rDNA sequence analysis combined with traditional morphological and physiological and biochemical characteristics, it was determined that strain D54 belonged to β-proteobacteria (Betaproteobacteria) Burkholderia sp.D54 (Burkholderia sp.D54), 2009 On August 12, it was deposited in the General Microorganism Center of China Microbiological Culture Collection Management Committee, and its preservation number is CGMCC No.3223. The inoculum prepared by inocul...

Embodiment 2

[0023] Embodiment 2: the preparation of the plant growth-promoting bacteria preparation provided by the present invention

[0024] The above Burkholderia (Burkholderia sp.D54) containing sterilized liquid modified SMN medium (glucose, 1.0g; sucrose, 1.0g; sodium acetate, 1.0g; sodium citrate, 1.0g; malic acid , 1.0g; (NH 4 ) 2 SO 4 , 2.0g; KH 2 PO 4 , 2.0g; K 2 HPO 4 , 0.2g; MgSO 4 , 0.2g; CaCl 2 , 0.1g; FeSO 4 , 5 mg; H 3 BO 3 , 2mg; ZnSO 4 , 5 mg; Na 2 MoO 4 , 1mg; CoSO 4 , 1mg; CuSO 4 , 1mg; NiSO 4 , 1mg; pH 7.2±0.02) 28°C, 150r / min, cultivated for 4 days, added sterilized peat (21% v / v), humus (20% v / v), adhesive methylcellulose (10% v / v) Concentrate and prepare liquid preparations of different specifications in sealed packages, and the number of effective viable bacteria reaches 1-2 billion / ml.

[0025] The powdery solid preparation used in the present invention is prepared from the above-mentioned liquid preparation through low-temperature freeze-drying...

Embodiment 3

[0026] Embodiment 3: the application method of the plant growth-promoting bacteria preparation provided by the present invention

[0027] Before sowing crop seeds, the effective live bacteria quantity prepared by embodiment 2 reaches 100 times (10ml / L, adds above-mentioned liquid preparation 10ml) after diluting 100 times (10ml / L, every L water adds above-mentioned liquid preparation 10ml) back soaking 1- After 2 hours, after the seeds germinate for 2-3 weeks, water the above-mentioned 100-fold diluted liquid preparation 1-2 times, which can effectively increase the probability of live bacteria infection. The application method of the freeze-dried preparation is to dissolve the powdered solid preparation with an effective viable count of 1 billion / g in water (add 1 L of water per 10 g of the above solid preparation), and the subsequent steps are the same as the liquid preparation above.

[0028] Surface disinfection (10%NaClO, 1 hour) each 100 of ryegrass, rapeseed, alfalfa seed...

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Abstract

The invention relates to heavy metal resistance plant growth-promoting bacteria and a preparation applying method thereof, which belongs to the bioremediation field of heavy metal polluted environment. A bacteria strain D54 with a preservation number of CGMCC No. 3223 belongs to the Burkholderia sp. and has higher resistance to a plurality of heavy metals, wherein the resistances to Pb <2+>, Cd <2+>, Cu <2+>, Zn <2+> respectively reach 800 mg/L, 1500 mg/L, 150 mg/L and 2500 mg/L. In addition, the bacteria strain D54 has the plant growth-promoting functions of producing plant growth hormone (IAA), producing 1-amino-1-carboxyl cyclopropane (ACC) deaminase, secreting siderophore, dissolving inorganic phosphate, fixing nitrogen and the like, has the biological prevention functions of antagonizing plant pathogenic bacteria inbreak and the like, and can obviously improve the biomass of the plants applied the invention and improve the resistance to diseases and stresses. The number of the effective viable bacteria in liquid preparation reaches 1-2 billion/ml and the number of the effective viable bacteria in solid preparation reaches 1 billion/g. Soaking seeds for 1-2 hours in the liquidpreparation which is diluted 100 times and irrigating the diluted liquid preparation 1-2 times (10ml/kg) after 2-3 weeks of the sprouting of the seeds can effectively improve plant viable bacteria infection probability.

Description

【Technical field】: [0001] The invention belongs to the technical field of bioremediation of heavy metal-contaminated environments, and in particular relates to a plant growth-promoting bacteria preparation resistant to heavy metals lead, cadmium, copper and zinc and an application method thereof. 【Background technique】: [0002] With the large-scale development and utilization of heavy metal mineral resources and fossil fuels, the massive discharge of industrial "three wastes" and the heavy use of heavy metal-containing pesticides and fertilizers have caused serious pollution to the ecological environment. The traditional treatment methods for heavy metal pollution are mostly physical and chemical methods. Such as chemical precipitation method, ion exchange method, reverse osmosis extraction method, activated carbon adsorption method, etc., they have their own advantages, but there are disadvantages such as large investment, high energy consumption, easy to cause secondary p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/02A01P21/00A01P3/00C12R1/01
Inventor 郭军康唐世荣宋正国居学海丁永祯赵玉杰
Owner AGRO ENVIRONMENTAL PROTECTION INST OF MIN OF AGRI
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