68 results about "Burkholderia sp" patented technology

The invention relates to a heavy metal lead and cadmium antibiotic-resistant bacterium and a method for strengthening lead and cadmium heavy metals in the vegetable extraction soil, and belongs to the agricultural and environmental pollution treatment technical field. The J62 culture stain is appraised as the Burkholderia sp, has antibiotic resistance to a plurality of heavy metals, in particular to lead and cadmium, respectively reaching Pb<2+>1000mg / L and Cd<2+>2000mg / L. The invention has the characteristics of nitrogen fixation and phosphate solubilizing, and can promote the growth of the plant and improve the stress resistance of the plant. The effective living microbial numbers of the liquid preparation are above a billion per millimeter, and the effective living microbial numbers of the solid preparation are 200 millions per gram. When the seeds of the plant are seminated in humid soil containing heavy metals, 10 to 20mL of the bacteria liquid of 10<8> bacteria J62 / mLs is inoculated in each grams of soil once or in two times. The invention promotes the growth of the plant, strengthens the enrichment of heavy metal lead and cadmium of the plant, and improves the extraction and recovery efficiency of the plant.

The invention relates to heavy metal resistance plant growth-promoting bacteria and a preparation applying method thereof, which belongs to the bioremediation field of heavy metal polluted environment. A bacteria strain D54 with a preservation number of CGMCC No. 3223 belongs to the Burkholderia sp. and has higher resistance to a plurality of heavy metals, wherein the resistances to Pb <2+>, Cd <2+>, Cu <2+>, Zn <2+> respectively reach 800 mg / L, 1500 mg / L, 150 mg / L and 2500 mg / L. In addition, the bacteria strain D54 has the plant growth-promoting functions of producing plant growth hormone (IAA), producing 1-amino-1-carboxyl cyclopropane (ACC) deaminase, secreting siderophore, dissolving inorganic phosphate, fixing nitrogen and the like, has the biological prevention functions of antagonizing plant pathogenic bacteria inbreak and the like, and can obviously improve the biomass of the plants applied the invention and improve the resistance to diseases and stresses. The number of the effective viable bacteria in liquid preparation reaches 1-2 billion / ml and the number of the effective viable bacteria in solid preparation reaches 1 billion / g. Soaking seeds for 1-2 hours in the liquidpreparation which is diluted 100 times and irrigating the diluted liquid preparation 1-2 times (10ml / kg) after 2-3 weeks of the sprouting of the seeds can effectively improve plant viable bacteria infection probability.

The invention discloses a burkholderia pyrrocinia JK-SH007 which is classified and named as burkholderia pyrrocinia and preserved in China center for type culture collection (CCTCC) with the preservation number of M 209028 and the preservation date of February 18th, 2009. The invention also discloses the application of the burkholderia pyrrocinia in controlling dothiorella gregaria and promoting poplar to grow. The invention further provides a bactericide for controlling dothiorella gregaria, which is obtained by inoculating the JK-SH007 in a culture medium to be fermented and cultured. The JK-SH007 is plant endophyte which has strong inhibiting effect for botryosphaeria dothidea of the poplar and antagonism for various pathogenic fungis, and has the advantages of simple culture condition, easy storage and commercial process as well as good developing and applying prospect.

The invention provides an autonomously-screened efficient phosphate-dissolving bacterium and a produced bacterial agent thereof, and belongs to the technical field of biology. The bacterial strain of the phosphate-dissolving bacterium is named as 21-III, is identified as burkholderia sp., and is preserved in China General Microbiology Center of Committee for Culture Collection of Microorganisms, and the preservation number is CGMCC No. 7873. 16SrDNA sequence of the bacterial strain is submitted to GenBank, and the registration number is JX857813. The main biological characteristics of the bacterial strain comprise that Gram staining is negative, thallus is in the shape of a rod, catalase reaction is positive, oxidase reaction is positive and methyl red reaction is negative. The bacterial agent has obvious promotion effect on dissolution rate of phosphorus in soil, and is applicable to improve soil fertility of crops and beneficial for environment protection.

The invention relates to a Burkholderia sp. and an application thereof. The invention relates to a Burkholderia sp., which is classified and named as Burkholderia sp., and is preserved in China General Microbiological Culture Collection Center with a preservation number of CGMCC No.16428 on September 6, 2018. The application of the Burkholderia sp. in promoting crop growth specifically comprises the step of preparing the Burkholderia sp. into a microbial seed coating agent. The Burkholderia sp. and the application thereof disclosed by the invention have the following beneficial effects: 1, theBurkholderia sp. can promote crop growth and synthesize growth hormones required by plant growth; 2, stable growth of crops can be kept in an acid-base environment within a certain range; 3, phosphorus in the soil can be converted into available phosphorus for plants, so that the planting cost is reduced, and meanwhile, the environment is protected; and 4, the growth of pathogenic bacteria can beinhibited.

The invention discloses Burkholderia cepacia P10 and application thereof. The Burkholderia cepacia P10 is named Burkholderia cepacia, and is collected at the China General Microbiological Culture Collection Center on March 18, 2019, wherein the collection number is CCTCC NO: M 2019172. The Burkholderia cepacia P10 has the functions of dissolving inorganic phosphorus, producing ACC deaminase and secreting iron carriers, has great potential in promotion of plant growth and development of microbial agents, and especially, has an obvious beneficial effect on the growth promotion of peanuts.

The invention discloses burkholderia and an application method thereof. The preservation number of a strain Burkholderia sp. Z-90 is CGMCC No.9970. The strain can produce a large amount of glycolipid biosurfactants in the metabolic process, and the biosurfactants can complex and desorb heavy metals in contaminated soil. The strain is used for treating soil contaminated by heavy metals, the contents of heavy metals such as Zn, Pb, Mn, Cd, Cu and As in the soil are respectively reduced from original 22374.1 mg / kg, 2472.4 mg / kg, 1837.2 mg / kg, 447.0 mg / kg, 709.5 mg / kg and 562.2 mg / kg to 12525.4 mg / kg, 1670.0 mg / kg, 878.1 mg / kg, 278.3 mg / kg, 538.6 mg / kg and 384.3 mg / kg, and the removal rates are respectively 44.0%, 32.5%, 52.2%, 37.7%, 24.1% and 31.6%.

The invention discloses a high-thermal-stability mutant of D-allulose-3-epimerase and an application of the high-thermal-stability mutant of D-allulose-3-epimerase. The 116th bits of serine of the mutant at D-allulose-3-epimerase is mutated to isoleucine, and the 251st bits of lysine is mutated to leucine, and a double mutant enzyme S116I / K251L is obtained. According to the invention, the 116th bit of serine (Ser, S) of D-allulose-3-epimerase BsDPE of Burkholderia sp. MR1 is mutated to isoleucine (Ile, I), and the 251st bits of lysine (Lys, K) is mutated to leucine (Leu, L), thus the thermal stability of the double mutant enzyme S116I / K251L obtained therefrom is significantly improved; and the half-life period at 60 DEG C is improved from the wild type 337.3 min to 417.9 min at present, and is 1.24 times o the wild type; the using cycle of the enzyme in compounding D-allulose is largely prolonged; the high-thermal-stability mutant of D-allulose-3-epimerase has important industrial application value.

The invention belongs to the field of the microorganisms and particularly relates to burkholderia LH-ZY01 (burkholderiasp.), and also relates to a strain and application thereof in suppressing rhizoctonia solani. The strain, namely the burkholderia LH-ZY01 (burkholderiasp.), is preserved in CCTCC (China Center For Type Culture Collection) on December 18, 2012; the preservation number of strain is CCTCCNO: M2012536. The burkholderia LH-ZY01 is combined with a loose nutrition carrier and the fermentation technology and the drying technology are adopted, so that the burkholderia is capable of growing in the nutrition carrier, and good in late-time stability and long in retention period. If the strain provided by the invention is applied to soil of crops infected by rhizoctonia solani such as rice, cotton, rape and tomato, diseases caused by the pathogenic bacteria can be remarkably eliminated; the efficiency of the strain for rice sheath blight disease is more than 75% and the efficiency for damping-off of colza is more than 59%; the diseases can be fundamentally eliminated from the soil to which the fertilizer is applied continuously for three crops.

The present invention provides genes that encode the N-acetyl-(R,S)-β-amino acid acylases. The N-acetyl-(R,S)-β-amino acid acylases were isolated and purified from bacterial cells and the nucleotide sequences were determined. A host, such as Escherichia coli, was used to construct a high-expression system for these genes. The N-acetyl-(R)-β-amino acid acylase produced by Burkholderia sp. AJ110349 (FERM BP-10366) includes, for example, the protein having the amino acid sequence shown in SEQ. ID. NO. 8. The gene encoding this enzyme includes, for example, the DNA having the nucleotide sequence as shown in SEQ. ID. NO. 7. The N-acetyl-(S)-β-amino acid acylase produced by Burkholderia sp. AJ110349 (FERM BP-10366) includes, for example, the protein having the amino acid sequence shown in SEQ. ID. NO. 10. The gene encoding this enzyme includes, for example, the DNA having the nucleotide sequence shown inshown in SEQ. ID. NO. 9. The N-acetyl-(R)-β-amino acid acylase produced by Variovorax sp. AJ110348 (FERM BP-10367) includes, for example, the protein comprised of the amino acid sequence shown in SEQ. ID. NO. 12. The gene encoding this enzyme includes, for example, the DNA having the nucleotide sequence shown inshown in SEQ. ID. NO. 11.

The invention belongs to the technical field of environment pollution biological treatment, relates to a microorganism repair technology and in particular relates to a method for enrichment and removal of caesium polluted environmental microorganisms. In the method, heavy metal resisting strain Burkholderia sp.D54 selected and separated from soil polluted by heavy metal is applied to repair of microorganisms polluted by radionuclide caesium. The strain Burkholderia sp.D54 can be grown in a culture medium containing 50mM of caesium; the strain Burkholderia sp.D54 is cultured in a culture medium containing 1mM of caesium for 3 days so that 0.1737+ / -0.0017g of thallus by dry weight; and the enrichment amount of the caesium reaches 45+ / -3.85mg g<-1> (dry weight thallus), and the removal rate of the caesium in the culture medium is 58.77%. The strain Burkholderia sp.D54 has strong appetency on radionuclide and can be applied to the repair of the environmental microorganisms polluted by the radionuclide caesium, thus the prospect of the strain is wide.

The invention discloses a Burkholderia sp. Strain ES-89 for preventing and treating fungal diseases of plants as well as a culture method and application. The culture method comprises the following steps: A, inoculating an ES-89 strain into a nutrient broth culture liquid for activation; B, inoculating the activated ES-89 into a triangular flask with the nutrient broth culture liquid, and performing shake cultivation; C, centrifuging fermentation broth obtained from shake cultivation under a certain condition, and filtering an upper layer of fermentation broth by a bacterial filter to obtain sterile supernate; D, washing the bacterium with sterile water to prepare a bacterial suspension, and storing the fermentation broth, the fermentation supernate and the bacterial suspension. The strain is preserved with the preservation number of CCTCC, CCTCC NO: M 2016665, and the strain ES-89 can be applied to preparation of medicines for treating or preventing plant pathogenic fungi. The strain has an inhibition function on coptis chinensis leaf spot diseases and the like, and can be applied to prevention and treatment on multiple plant diseases. The prevention and treatment effect of the strain on the coptis chinensis leaf spot diseases can be 88% or greater, the culture method is feasible, the operation is simple and convenient, the pollution in the production process is reduced, solvent poisoning is avoided, and the application prospect is relatively good.

The invention discloses a rice endotrophic azotobacter for producing activated calcium carbonate (ACC) and realizing the antagonistic effect on fusarium graminearum and a purpose thereof. The rice endotrophic azotobacter is Burkholderia sp. GDSD125 and has the preservation number being CGMCC No.5038 in General Microbiology Center of China Microbial Strain Preservation Management Committee. The Burkholderia sp. GDSD125 CGMCC No.5038 provided by the invention has high nitrogen fixation activity and ACC ammonialyase activity, can realize the effective antagonistic effect on gibberella zeae and has wide application prospects in nitrogen fixation microbial agent and microbial organic fertilizer production.

The invention belongs to the technical field of microorganisms, and specifically discloses a Burkholderia kururiensis strain and application thereof. The strain is Burkholderia kururiensis yy01, and is preserved in China General Microbiological Culture Collection Center (CGMCC) on March 17, 2015, and has preservation No. CGMCC No. 10631. The strain yy01 has both efficient releasing of phosphorus and potassium and high nitrogenase activity, increases the effectiveness of insoluble phosphorous and potassium in soil and convert free nitrogen in the air into ammonia for absorption and utilization by crops, and has significant growth-promoting effect on rice, tobacco and other crops.

The invention relates to a preparation method and application of burkholderia MEL01 capable of efficiently antagonizing fusarium graminearum, and belongs to the technical field of microorganisms. Themethod comprises the following steps: carrying out shake-flask culture on Burkholderia sp. MEL01 for 2 days and then carrying out static culture for 3 days, or continuously carrying out shake-flask culture for 3 days after carrying out shake-flask culture for 2 days; adding fusarium graminearum hyphae every day continuously for three times. The inhibition activity of the supernatant obtained by the fermentation method on fusarium graminearum hyphae is obviously improved; when the method is used in the early flowering stage of wheat, the disease index and DON synthesis can be remarkably reduced; and the method is simple and easy to operate, and the antibacterial activity of the Burkholderia sp. MEL01 (Burkholderia sp.) fermentation supernatant can be remarkably improved.

A DNA fragment (8050 bp) for the 2-naphthalene acid degradating bacterium (Burkholderia sp. JT1500) and its preparing process are disclosed. Its prokaryotic expression plasmid is configured. Its recombinant bacterium strain containing said DNA fragment is obtained by transferring to cobacillus, which can effectively degradating 2-naphthalene acid and sodium benzoate, so degradating the environmental pollutants.

A composite bacterium agent for reducing a doxycycline concentration and a drug-resistant gene in a composting process and applications thereof are disclosed and belong to the technical fields of antibiotics and drug-resistant gene removing. The composite bacterium agent includes Ochrobactrum sp., Burkholderia sp., and Candida sp.. The mass ratio of freeze-dried powder of the Ochrobactrum sp., theBurkholderia sp., and the Candida sp. is 1-2:1-2:1-2. Through adding the composite bacterium agent into compost, residual doxycycline in livestock excrement can be efficiently degraded, the drug-resistant gene residual in the compost is effectively degraded, and safety of a compost product is improved. Through inoculation of the composite bacterium agent, the doxycycline degradation rate is 46.40%, and the tetracycline drug-resistant gene removal rate is 38.71%. Compost inoculated with the composite bacterium agent has higher doxycycline degradation effects and a lower drug-resistant gene content than compost not inoculated with the composite bacterium agent so that the composite bacterium agent has a good application prospect.

Burkholderia sp. for aerobic degradation of benzpyrole and the application of burkholderia sp. belong to the technical field of microorganism. The strain separated from activated sludge of a reactor is preserved in the Center for General Microorganisms of China Committee for Culture Collection of Microorganisms (CCCCM) on January 28, 2015, with the preservation number of CGMCC No.,10457. 16S Rrna gene sequence determination shows that strain IDO3 and Burkholderia have high similarity, therefore, the strain is classified and named as Burkholderia sp. IDO3, and the accession number of the gen bank of the gene sequence of the strain 16S rRNA is KP895480. The strain has a relatively stronger degradation capacity to benzpyrole, also has excellent Ph and temperature tolerances, and shows the application prospect in treating waste water with high concentration benzpyrole.

The present invention discloses a strain of Burkholderia sp.CG-01 (CGMCC No.7329) and a method for fermentation synthesis of polyhydroxyalkanoate by adopting the Burkholderia sp.CG-01. According to the present invention, the wild bacterial can adopts glucose as a carbon source to synthesize PHB, wherein the PHB content is 37.55% of the dry weight of the bacterial without optimized conditions; and the bacterial can grow on more than 100 kinds of carbon source culture mediums, such that great development spaces are provided for further fermentation condition optimization, yield increase and PHA production cost reduction.

The invention discloses a method for forming a biomembrane by using burkholderia pyrrocinia. The method comprises the following steps: 1) preparing single colonies from an LB solid slab activated burkholderia pyrrocinia JK-SH007 strain, selecting the single colonies, putting the single colonies into an LB liquid culture medium, and performing pure culture for 24 hours at 180r / min at 28 DEG C so asto obtain a bacterial suspension; 2) preparing a test culture medium TMG, and adding 1%v / v glycerinum and 25mM of MgSO4 into the TSB (trypticase soy broth) culture medium, wherein the pH value is 5-7; and 3) inoculating the 1%v / v bacterial suspension into the TMG liquid culture medium, performing pure culture for 24 hours at 180r / min at 28 DEG C, taking out the solution, and allowing the solutionto stand for 4-6 days, so as to form the biomembrane. By adopting the method, the JK-SH007 can be induced to form the culture medium TMG of the biomembrane, after culture with the culture medium, theJK-SH007 can form the biomembrane which is visible by naked eyes, and thus the JK-SH007 can survive well in an environment in nutrition deficiency.

The invention discloses a Burkholderia sp. and application thereof, and belongs to the field of microorganisms. The Burkholderia bacterium Burkholderia sp. MEL01 provided by the invention had been collected in the China Center for Type Culture Collection with a collection number of CCTCC NO.M2015778 and on a collection date of December 25, 2015. The bacterial strain has higher chitosanase activity, and further has obvious antagonistic actions on six vegetal pathogenic fungi of a strawberry gray mold and the like; by utilizing a characteristic that the bacterial strain MEL01 can degrade chitosan, the MEL01 is mixed with colloidal chitosan to make the obtained mixture into composite bacterial fertilizer which can obviously improve the soil fertility of a crop; the Burkholderia sp. can be applied to the biological prevention and control of a vegetal pathogenic bacterium and the production of novel biological fertilizer, and has a wide application prospect in an aspect of ecological agriculture.

The invention belongs to the technical field of microorganism, and particularly relates to a Burkholderia FH4 bacterial strain, and a screening method and application thereof. The preservation number of the Burkholderia FH4 bacterial strain is CGMCC No.8271; the application method of enzymolysis Enteromorpha of the bacterial strain comprises the steps: performing excitation culture on a cryopreserved FH4 bacterial strain in an LB slant culture medium, then selecting and putting a complete ring into an LB culture liquid medium, performing shaking culture for 24 to 28 h, putting 1 to 2 mL of a bacterial solution into cellulose liquid fermentation culture medium subjected to sterilization, performing shaking culture for 5 to 7 days, then performing low-temperature centrifugation on fermentation liquor, using supernate as crude enzyme, or filtering the fermentation liquor, and using filter liquor as crude enzyme. The crude enzyme reacts with clean Enteromorpha powder at 45 to 55 DEG C. The Burkholderia FH4 bacterial strain has the capacity of producing cellulose, can effectively degrade cellulose substance in Enteromorpha into reducing sugar, and is low in production cost, simple in equipment, mild in reaction and applicable to large-scale industrial production.

The invention discloses a Burkholderia sp.HDXY-02 capable of being stably produce toxoflavin with a high yield, and belongs to the technical field of applied microorganisms. The strain is preserved onApril 20, 2017 in China General Microbiological Culture Collection Center (abbreviation CGMCC), and the preservation number is CGMCC No.14054. After the strain is subjected to fermentation in a liquid culture medium or spread plate culture in a solid plate for 48-72 h, extraction is performed by dichloromethane or chloroform, separation purification is performed to obtain the toxoflavin, and thetoxoflavin has the activity of inhibiting pathogenic bacteria aspergillus fumigatus and resisting tumor Lovo cells. The strain provided by the invention can synthesize the toxoflavin by fermentation,and the toxoflavin has a high yield; and compared with a current chemical synthetic method, the strain provided by the invention avoids the pollution to the environment by chemical substances, and haspotential application prospects.

The invention discloses an engineering bacteria strain for heterogenous expression of a histone deacetylases inhibitor Thailandepsin A. The bacterial strain is named as the engineering bacterial strain 7029-tdp-A, which uses Burkholderia sp.DSM7029 as an original strain, and a biosynthetic gene cluster (tdp) of Thailandepsins is integrated on a genome through a transposition mode. The invention also discloses an application of the engineering bacterial strain in preparation of the histone deacetylases inhibitor Thailandepsin A. The experiment proves that the engineering bacterial strain 7029-tdp-A and a wild bacterial strain Burkholderia thailandepsis E264 are compared, the output of Thailandepsin A is increased by one time, and the engineering bacteria strain establishes a base for large-scale preparation and exploitation of the histone deacetylases inhibitor drugs.

The invention discloses a burkholderia strain and application thereof. The strain is named as Burkholderia sp. YCYP9 in taxonomy, and the preservation number of the strain is CCTCC (China Center For Type Culture Collection) NO: M 2021733. The strain has the function of simultaneously dissolving inorganic phosphorus and organic phosphorus, and can be used for preparing a phosphorus-dissolving preparation. Meanwhile, the strain also has a function of promoting the growth of camellia oleifera seedlings, and can be used for preparing a camellia oleifera seedling growth promoter.

The invention discloses a bacterial strain resistant to acid-aluminum. The bacterial strain belongs to beta-proteobacteria burkholderia sp., is named as Burkholderia sp.SB1, and is collected in China General Microbiological Culture Collection Center (CGMCC) in September, 2013, with the collection number of CGMCC No. 8396. The bacterial strain has beneficial effects that the bacterial strain shows stronger acid-aluminum resistance performance, and has stronger adsorption effect on aluminum, so that a good microbial material is further provided for improving soil contaminated by aluminum by utilizing the bacterial strain.

The invention discloses a bromoxynil degrading bacterium and application thereof, and belongs to the field of high biotechnologies. A bacterial strain of the bromoxynil degrading bacterium is a Gram staining reaction negative bacterium BX03, and belongs to Burkholderia bacterial genus (Burkholderia sp.) is identified as Burkholderia sp.. The bromoxynil degrading bacterium is mainly biologically characterized in that the bromoxynil degrading bacterium is Gram-negative, is in the shape of a short spar and is free of spores, and flagella grow at the ends of the bromoxynil degrading bacterium. Bromoxynil can be used as the only carbon source and the only nitrogen source to grow the bacterium, and the bacterium can be mineralized. The bromoxynil degrading bacterium and the application have the advantages that 95% of 100mg / L of the bromoxynil can be degraded by the bacterial strain within 24 hours under a flask shaking condition in a laboratory, and accordingly the problem of difficulty in biologically degrading bromoxynil during waste water treatment can be solved.

The invention provides a fermentation strategy capable of improving toxoflavin production of burkholderia sp.HDXY-02, and belongs to the technical field of microbial fermentation. A strain HDXY-02 ispreserved in China General Microbiological Culture Collection Center, and the preservation number is CGMCC No.14054. The strategy determines that the optimal inoculation amount is 3% and the culture temperature is 30 DEG C. Through single factor experiments and statistical analysis, the optimal culture medium conditions are determined as follows: glucose (19.24 g / L), peptone (20.45 g / L), phenylalanine (0.472 g / L), K2HPO4 (1.5 g / L), MgSO4 (0.75 g / L), and pH 7.0. After optimization, the toxoflavin yield reaches 1533 mg / L and is improved by 296% than that before optimization, and the feasible fermentation strategy is provided for the high production of toxoflavin by a microbial fermentation method.

The invention discloses a microorganism microbial agent for promoting growth of rice and reducing cadmium and a preparation method and use method of the microorganism microbial agent, and belongs to the technical field of government of heavy metal pollution with microorganisms. The microorganism microbial agent provided by the invention comprises listeria grayi, burkholderia sp., pseudomonas stutzeri, bacillus subtilis and micromonospora echinospora, and is prepared through the steps of performing strain activation, performing enlarged culture, performing fermentation culture and performing mixing. At the growth resuming period during growth of the rice, the microorganism microbial agent is added to rice water culture potting nutrient fluid in the volume ratio of 1 to 400, and culturing isperformed until the highest tillering stage is achieved. Under the condition of the percentage by quantity of the components of the microorganism microbial agent that the listeria grayi is 50%, the burkholderia sp. is 20%, the pseudomonas stutzeri is 10%, the micromonospora echinospora is 10% and the bacillus subtilis is 20%, the absorptivity of the heavy metal cadmium by the rice is the lowest,and the potential of promoting growth of the rice is achieved. The microorganism microbial agent prepared by the preparation method disclosed by the invention has positive influence on promoting growth of the rice and reducing the absorption of the cadmium by the rice, and has good application prospects.