Burkholderia cepacia P10 and application thereof

A Burkholderia and onion technology, applied in the field of agricultural microbes, can solve the problems that Burkholderia strains have no peanut growth-promoting effect, and achieve plant height increase, growth promotion, and great potential Effect

Active Publication Date: 2019-06-21
GUIZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, there are few reports on the patents of Burkholderia strains, and there is no report on the growth-promoting effect of Burkholderia strains on peanuts

Method used

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  • Burkholderia cepacia P10 and application thereof
  • Burkholderia cepacia P10 and application thereof
  • Burkholderia cepacia P10 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1: Isolation and identification of Burkholderia cepacia P10

[0024] 1.1 Isolation of Burkholderia cepacia P10

[0025] Collect 10g of tea tree rhizosphere soil from the tea garden in Kuanshui Nature Reserve, Guizhou Province, and add it to 90mL NBRIP liquid medium for enrichment culture; The culture medium was transferred to a fresh medium with the same composition, and cultured under the same conditions. This process was repeated 4 times; 1 mL of the last culture medium was drawn and diluted, and spread on NBRIP solid medium for 7 days at a constant temperature of 30°C. The colonies with larger phosphorus-dissolving hydrolysis circles were picked and streaked and purified repeatedly on NBRIP plates until pure cultured strains were obtained, which were labeled as P10.

[0026] The medium formula of the above NBRIP is: glucose 10g, tricalcium phosphate 5g, MgCl 2 ·6H 2 O5g, MgSO 4 ·7H 2 O0.25g, KCl 0.2g, (NH 4 ) 2 SO 4 0.1g, distilled water 1000mL, pH7.0...

Embodiment 2

[0039] Embodiment 2: Determination of growth-promoting properties of Burkholderia cepacia (Burkholderia cepacia) P10

[0040] 2.1 Determination of Phosphorus Solubilizing Ability of Burkholderia cepacia P10

[0041] Qualitative determination of phosphate dissolution: Spot the activated P10 strain on NBRIP solid medium, and culture it upside down at 30°C for 7 days, see attached image 3, Determining the diameter of the colony and the diameter of the phosphorus-dissolving hydrolysis circle, and calculating the ratio of the diameter of the phosphorus-dissolving hydrolysis circle / the diameter of the colony to be 3.29.

[0042] Quantitative determination of phosphate dissolution: Transfer the activated P10 bacteria solution to 100mL NBRIP liquid medium, culture it on a shaking table at 30°C for 7 days, take the culture solution and centrifuge at 10,000rpm at 4°C for 10min to measure the pH value, and use molybdenum blue colorimetry The soluble phosphorus content in the supernatan...

Embodiment 3

[0056] Embodiment 3: Burkholderia cepacia P10 is to the growth-promoting determination of peanut

[0057] Preparation of P10 inoculum solution: Inoculate the P10 strain into NA liquid medium at 30°C and shake at 150rpm for 24 hours, collect the bacteria by centrifugation and resuspend them with an appropriate amount of sterile water, adjust the OD600 value to 0.5 (the number of bacteria is about 10 8 cfu / mL) for use.

[0058] The formula of the above NA medium is: beef extract 5g, peptone 10g, sodium chloride 5g, distilled water 1000mL, pH7.0-7.2, sterilized at 121°C for 20min.

[0059] Soak peanut seeds in 20% hydrogen peroxide solution for 20 minutes for surface disinfection, rinse with sterile water several times, put them on sterilized filter paper moistened with distilled water, and germinate them in a constant temperature incubator at 30°C for about 3-4 days. Peanut seedlings were transplanted into seedling pots. Two treatments were set up in the experiment, the P10 st...

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Abstract

The invention discloses Burkholderia cepacia P10 and application thereof. The Burkholderia cepacia P10 is named Burkholderia cepacia, and is collected at the China General Microbiological Culture Collection Center on March 18, 2019, wherein the collection number is CCTCC NO: M 2019172. The Burkholderia cepacia P10 has the functions of dissolving inorganic phosphorus, producing ACC deaminase and secreting iron carriers, has great potential in promotion of plant growth and development of microbial agents, and especially, has an obvious beneficial effect on the growth promotion of peanuts.

Description

technical field [0001] The invention belongs to the technical field of agricultural microorganisms, and relates to a strain of Burkholderia cepacia P10 and its application. Background technique [0002] Plant rhizosphere growth-promoting bacteria are a kind of beneficial bacteria that grow in the rhizosphere soil of plants. They have various growth-promoting mechanisms for plants, such as biological nitrogen fixation, dissolving phosphorus and potassium, producing plant hormones such as IAA, antagonizing pathogenic bacteria, and inducing systemic resistance. sex etc. In particular, the organic acids produced by these bacteria during the metabolic process can dissolve insoluble phosphorus and potassium in the soil to promote the growth and development of plants, and can also enhance plant resistance to diseases, heavy metals and salt by secreting siderophore and ACC deaminase. Resistance to adversities such as alkalis. It is of great significance to isolate and screen micro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P21/00C12R1/01
Inventor 韩丽珍黄文茂周静
Owner GUIZHOU UNIV
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