35 results about "ACC deaminase" patented technology

The invention discloses a medicago pseudomonas strain capable of producing ACC (1-aminocyclopropane-1-carboxylate) deaminase and application of the medicago pseudomonas strain, and relates to a medicago pseudomonas strain and application thereof. The pseudomonas (pseudomonas sp.) MJM-9 is preserved in the general microorganism center of the China microorganism culture preservation management committee in 26th July, 2012, and the preservation number of the pseudomonas MJM-9 is CGMCC NO.6294. The medicago pseudomonas strain containing the ACC deaminase is used as bacterial manure for growing crops. The medicago pseudomonas strain can generate the ACC deaminase, secrete auximone IAA (indole acetic acid), synthesize siderophores, reduce the concentration of ethylene inside a plant under the condition of environmental stress, promote the plant to grow fast, particularly promote growth and development of a root system of the plant, and increase utilization rate of fertilizers. Besides, when the strain is applied to agricultural production, growth of plants can be promoted, yield of crops can be increased, and stress resistance of the crops is improved.

The invention discloses endogenous growth-promoting rhizobium of rices and application thereof. The endogenous growth-promoting rhizobium of the rices is rhizobium oryzihabitans, the strain number isM15, and the registration number of China General Microbiological Center of Microbiological Culture Collection Committee is CGMCC No.15530. Proved by experiments, rhizobium oryzihabitans M15 can generate IAA, every liter of fermenting liquid with the OD600nm value being 1 contains 85.32mu g IAA. The rhizobium oryzihabitans M15 can generate ACC deaminase, the activity of the ACC deaminase of the rhizobium oryzihabitans M15 is 4.86mu mol alpha-butanone acid/h.mg protein.

The invention discloses a heavy-metal-resistant rhizobium namely rhizobium HZ76. The rhizobium is applied to promoting plants to repair heavy metal polluted soil. The effective bacterium number of rhizobium HZ76 strains is (10<8>-10<9>) CFU/mL. A plant adopted by plant repair of the heavy metal polluted soil is locust tree. The heavy metals in the heavy metal polluted soil comprise one or more of Cu, Zn, Pb and Cd. The HZ76 strain disclosed by the invention can produce indoleacetic acid which is beneficial to promoting plant growth. The heavy-metal-resistant rhizobium has an ACC deaminase gene and nod and nif genes, which are helpful for improving the nodulation rate and nitrogen content of plants. The heavy-metal-resistant rhizobium can generate an iron carrier which is helpful for improving the stress resistance of the plants. Plant growth promoting bacteria is used for promoting the growth of the plants, improving the content of the heavy metals Zn, Pb and Cu in the plants, and strengthening the plant extraction repair efficiency.

The invention discloses a microbial preparation for soil remediation. The microbial preparation comprises a porous support and a composite microbial agent loaded in the porous support. The porous support comprises diatomite, calcium sulfate and sodium alginate. The composite microbial agent comprises an Arthrobacter sp. LHM7719 microbial agent, a Rhodopseudomonas palustris microbial agent and ACCdeaminase. The Arthrobacter sp. LHM7719 microbial agent is used for the field of soil remediation, and is composited with the Rhodopseudomonas palustris microbial agent and ACC deaminase to obtain a microbial soil remediation agent which can regulate soil pH value and reduce soil hardening, reduce content of heavy metal in soil and degrade organic pollutant in soil.

The invention relates to plant growth-promoting rhizobacteria Gro (rahnella aquatilis) and application thereof. The plant growth-promoting rhizobacteria Gro is classified and named as rahnella aquatilis, and the preservation number is CGMCC NO.15411. The bacterial strain can produce heteroauxing at high yield; the effect that insoluble potassium-containing silicate is converted into soluble potassium salt is effectively achieved; the organic phosphorus is dissolved; meanwhile, the growth promoting characteristics of fixing nitrogen and generating ACC deaminase, gibberellin, siderophore and thelike are realized; the soil nutrients can be increased; the utilization rate of fertilizers is improved; the absorption of plants on the soil nutrients sis promoted; the bacterial strain can be particularly and successfully subjected to fix planting in cucumber and lettuce rhizosphere soil; the growth of cucumbers and lettuces is promoted; the yield is improved. The bacterial strain Gro has the relatively comprehensive growth promoting function; the IAA producing capability is high; the extreme environment such as acid and alkaline is resisted; high application potential is realized.

The invention discloses a method for separating 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase growth-promoting bacteria produced by plant on copper tailings, and evaluating the performances of the 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase growth-promoting bacteria. Various strains for producing ACC deaminase are separated from the root system of the phytocoenosium growing on the copper tailings, and the evaluating for the performances and applications comprise the evaluating for the individual performances of the separated strains and the influences of the strains for the growth of native plants and the exotic plants. The separated various strains can secrete ACC deaminase, IAA deaminase and siderophore and have the phosphorus dissolubility, the separated strains can be used for repairing copper tailings areas, and the prepared microbial modifier has the great acceleration effect for the native plants and exotic plants growing on the copper tailings.

The invention discloses a soybean rhizosphere azotobacter for producing activated calcium carbonate (ACC) and realizing the antagonistic effect on various pathogenic fungi and a purpose thereof. The soybean rhizosphere azotobacter is Burkholderia sp. GDSD571 and has the preservation number being CGMCC No.5039 in General Microbiology Center of China Microbial Strain Preservation Management Committee. The Burkholderia sp. GDSD571 CGMCC No.5039 provided by the invention has high nitrogen fixation activity and ACC ammonialyase activity, can realize the effective antagonistic effect on gibberella zeae, sclerotinia sclerotiorum and verticillium dahliae and has wide application prospects in nitrogen fixation microbial agent and microbial organic fertilizer production.

The invention discloses a tea tree growth-promoting rhizobacterium of which the classification designation is Serratia marcescens. The strain number is JW-CZ2. The Serratia marcescens is collected by China Center for Type Culture Collection, the collection number is CCTCC M 2015095, and the collection date is March 10th, 2015. The invention also discloses application of the Serratia marcescens in promoting tea tree growth. The JW-CZ2 strain has high solubility for insoluble phosphates, such as tricalcium phosphate, magnesium phosphate and iron phosphate. The JW-CZ2 strain has IAA (indoleacetic acid) secretion capacity and ACC deaminase producing capacity. When a tea tree seedling plant is inoculated in the JW-CZ2 microbial inoculant, the microbial inoculant can obviously promote the growth of the tea tree seedling. Therefore, the tea tree growth-promoting rhizobacterium Serratia marcescens can provide an excellent strain resource for future development of special microbial fertilizers for tea trees.

The invention discloses a rice endotrophic azotobacter for producing activated calcium carbonate (ACC) and realizing the antagonistic effect on fusarium graminearum and a purpose thereof. The rice endotrophic azotobacter is Burkholderia sp. GDSD125 and has the preservation number being CGMCC No.5038 in General Microbiology Center of China Microbial Strain Preservation Management Committee. The Burkholderia sp. GDSD125 CGMCC No.5038 provided by the invention has high nitrogen fixation activity and ACC ammonialyase activity, can realize the effective antagonistic effect on gibberella zeae and has wide application prospects in nitrogen fixation microbial agent and microbial organic fertilizer production.

The invention specifically relates to enteroaerogen HBRM-86 and applicaton of the same as a rubber tree growth-promoting rhizobacterium strain, belonging to the technical field of plant microbes. According to the invention, ACC deaminase is used as a main index; the enteroaerogen HBRM-86 is obtained through elaborate screening and preserved in China Center for Type Culture Collection; and experimental results prove that the screened enteroaerogen HBRM-86 can effectively function as a growth-promoting rhizobacterium strain, benefits growth of the seedlings of a rubber tree and is favorable for increasing the output of natural rubber.

The present invention relates to a bio-preserving fungus agent for fruits and vegetables and its preparation method. The said preparation method for bio-preserving fungus agent is: (1) screening and obtaining the strain capable of using ACC from the fresh ethylene climacteric fruit; (2) inoculating and reisolating the strain obtained from (1) by anti-rifampicin marker method to select the strain capable of colonizating at the inside and surface of the fruits or vegetables; (3) purifying the enzyme activity of the said screened strain ACC deaminase, selecting the enzyme-activity unit above 0.2U/mg strain, and repeatedly 2 to 3 times inoculation and reisolation and determining the enzyme activity of ACC deaminase, selecting the enzyme activity and colonization keeping unchanged strain as object strain; (4) making the said (3) selected strain to fruits and vegetables bio-preserving fungus agent by rejuvenation of spawn etc. through the method of the present invention, it can obtain the fruits and vegetables preserving agent, delay the fruits ripening effectively, increase the preservation of fruits and vegetables.

The invention provides clostridium welchii. The preservation number of the clostridium welchii is CCTCC NO.M2019204. A microorganism is screened from a domesticated water hyacinth root by an inventor,it is found that the strain has very strong resistance to heavy metals, and the strain is identified as the clostridium welchii by colony morphology, biochemistry and 16S rDNA sequencing analysis. The clostridium welchii can improve the enrichment capacity of water hyacinths to heavy metals including at least one of Cd, Zn, Mn, Ni, Cu and Pb. Meanwhile, IAA and ACC deaminase can be released to promote the growth of the water hyacinths.

The invention relates to a novel strain of Halomonas sp. obtained by isolation and screening, which has the ability to secrete indoleacetic acid and produce ACC deaminase. The strain can grow well under low temperature or high temperature and salt and alkali conditions. The composition of the strain and a microbial fertilizer prepared by a liquid fermentation technique alleviate the salt-alkali stress caused by the salt on the crops and promote the growth of the crop under salt stress. The novel strain or the composition provided by the invention is suitable for special geographical and climatic conditions and improves the physical and chemical properties of the soil and the ability to fertilize, effectively utilizes environmental resources and reduces pesticide pollution. The novel strainor the composition has the characteristics of good effect, short cycle and good environmental compatibility, and has important practical value for solving the current soil salinization.

The invention discloses a preparation method of a phosphate-solubilizing microbial agent and application thereof and belongs to the field of microbial technology. The preparation method of the phosphate-solubilizing microbial agent comprises the following steps: (1) preparation of a liquid state phosphate-solubilizing microbial solution; and (2) preparation of a solid state phosphate-solubilizingmicrobial agent. The application of the solid state phosphate-solubilizing microbial agent comprises the following steps: (1) during building and planting, the solid state phosphate-solubilizing microbial agent is applied by a broadcasting mode, and the application amount is 10-20 kg/Mu; and (2) during sowing, the solid state phosphate-solubilizing microbial agent and seeds undergo seed dressing (1 kg of ryegrass seeds are stirred into 5-8 kg of the solid state phosphate-solubilizing microbial agent), and sowing is conducted. The phosphate-solubilizing microorganism, namely Aspergillus aculeatus has endophilicity with ryegrass, has capabilities of secreting IAA, siderophore, ACC deaminase and organic acid, can effectively promote plant growth while solubilizing phosphate, can improve soilfertility, and can greatly increase content of rapidly available phosphorus in soil. The preparation is convenient, application method is simple, and a broadcasting or seed dressing method, etc. can be adopted for inoculation; and cost is low, and operation is simple.

The invention provides an efficient plant growth promoting bacterium Ochrobactrum sp.NW-3 which is collected under number of CGMCC No.10858. The efficient plant growth promoting bacterium is used for producing hormone IAA, ACC deaminase and ironophore and is further used for producing products and bio-fertilizer for promoting plant growth. By the microbial fertilizer, soil pollution, water pollution and threat to human health caused by chemical fertilizer can be reduced greatly, soil can be improved, soil fertility can be enhanced, agricultural non-point source pollution can be reduced, green agriculture can be developed, and sustainable development of agriculture can be promoted, so that the bio-compound fertilizer has wide application prospect and huge market potential.

The invention relates to Mesorhizobium KDRM611 and the application of the Mesorhizobium KDRM 565. The Mesorhizobium KDRM611 is separated from Robinia pseudoacacia root nodule, contains ACC deaminase and can effective carry out nodulation and promote the growth of the Robinia pseudoacacia. The Mesorhizobium KDRM 611 strain is preserved in China Center for Type CultureCollection with the preservation number of CCTCC NO:M2012335. The Mesorhizobium KDRM 611 disclosed by the invention contains the ACC deaminase which can decompose ACC into alpha-ketobutyrate and NH3, reduce the level of synthesizing ethylene with plant cells, reduce an inhibition function of ethylene on rhizobium infection, improve the nodulation rate of rhizobium and the Robinia pseudoacacia and the level of symbiotic nitrogen fixation, provide high-level nitrogen for the growth of the Robinia pseudoacacia on non-fertilized barren wasteland, promote the growth of seedlings of the Robinia pseudoacacia and increase the biomass of mature the Robinia pseudoacacia, thereby realizing high yield of the mature Robinia pseudoacacia by using inoculation with low cost, and playing a role in Robinia pseudoacacia culture and forestation.

The application of the invention relates to the field of genetic modification and gene editing, in particular to genetic modification and gene editing in plants such as crops. The invention provides amethod for improving the drought resistance of the plants through ACC deaminase in specific expression. Through the adoption of the method disclosed by the invention, the drought enduring capacity ofthe plants, such as the crops especially corn, can be improved.

The invention discloses a preparation method and application of a potassium-dissolving microbial agent, and belongs to the field of microbial technology. The preparation method of the potassium-dissolving microbial agent comprises: 1, preparation of a liquid potassium-dissolving microbial liquid; and 2, preparation of a solid potassium-dissolving microbial agent. The application of the solid potassium-dissolving microbial agent includes: (1) during planting, the solid potassium-dissolving microbial agent is applied by a broadcast mode, and the application amount is 10-20 kg per acre; (2) during sowing, the solid potassium-dissolving microbial agent and seeds undergo seed dressing, and 5-8 kg of the solid potassium-dissolving microbial agent is mixed in 1 kg of ryegrass seeds for sowing. The potassium-dissolving microorganism, namely Aspergillus aculeatus, has compatibility with ryegrass, has the capability of secreting IAA, siderophore, ACC deaminase and organic acid, and can effectively promote growth of the plant while dissolving potassium, can improve the soil fertility, and can greatly increase the content of rapidly available potassium in the soil. The preparation is convenient, the application method is simple, and the product can be inoculated by means of broadcast or seed dressing. The cost is low and the operation is simple.