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Acinetobacter strain and application thereof in improving stress resistance of plants

A bacillus and plant technology, applied in the field of biological agriculture, can solve the problems of soil microbial diversity destruction, soil health status reduction, and soil microbial activity.

Active Publication Date: 2022-03-29
NORTHEAST FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, the physical and chemical properties of soil caused by salinity will affect the activity of soil microorganisms, resulting in the destruction of soil microbial diversity, thereby reducing the health of the soil

Method used

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  • Acinetobacter strain and application thereof in improving stress resistance of plants
  • Acinetobacter strain and application thereof in improving stress resistance of plants
  • Acinetobacter strain and application thereof in improving stress resistance of plants

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0106] Example 1. Isolation, Identification and Preservation of Acinetobacter sp. YJ-AD2

[0107] 1. Isolation of bacteria YJ-AD2

[0108] 1. Add 5g of soil samples (collected from the rhizosphere soil of plants in saline-alkali land near No. 6 Anfa Avenue, Anda Economic and Technological Development Zone, Suihua City, Heilongjiang Province) in 45mL sterile distilled water, stir for 15 minutes, let stand for 10 minutes, and then take the supernatant Add 1mL of solution into a sterile test tube filled with 9mL of sterile water and mix well (at this time, the dilution is recorded as 10 -1 ), then draw 1mL from this test tube and add it to another sterile test tube filled with 9mL sterile water and mix evenly, and so on to make 10 -2 、10 -3 、10 -4 、10 -5 、10 -6 、10 -7 Bacterial suspensions at different dilutions. 0.1 mL of each dilution was evenly spread on the LB solid medium, and cultured at a constant temperature of 30°C for 2-3 days.

[0109]2. After completing step 1...

Embodiment 2

[0133] The preparation of embodiment 2, YJ-AD2 bacterial agent

[0134] 1. Activate the Acinetobacter sp. YJ-AD2 strain stored at -80°C in Example 1 on LB solid medium.

[0135] 2. Pick a single colony from LB solid medium and inoculate it in a conical flask (500mL) filled with 100mL LB liquid medium, and culture it at 30°C and 130r / min for 48h to obtain OD 600nm Bacterial solution with a value of about 1.0 (2×10 8 • CFU / mL). The bacterial solution is the prepared YJ-AD2 microbial agent.

Embodiment 3

[0136] Embodiment 3, the application of Acinetobacter YJ-AD2 in improving the salt-alkali resistance of Arabidopsis

[0137] The size of the petri dish is 10cm×10cm.

[0138] 1. Preparation of culture medium

[0139] Alkaline solid medium: add NaHCO to MS solid medium 3 Adjust the pH value to 8.0 until the final concentration is 1.5mM; then sterilize at 121°C for 15min, pour the culture medium at about 55°C into petri dishes (25mL per dish), and cool naturally.

[0140] Alkali + bacterial agent solid medium: Take the petri dish containing the alkaline solid medium, apply 0.1mL of the YJ-AD2 bacterial agent prepared in Example 2 on the surface of the medium below the lower quarter, and let it dry naturally.

[0141] 2. The effect of YJ-AD2 bacterial agent on the resistance of Arabidopsis to saline-alkali stress

[0142] The culture conditions are: 22°C; 12h light / 12h dark; the light intensity is 5000Lx.

[0143] 1. Take Arabidopsis thaliana (wild-type Arabidopsis Columbia-0...

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Abstract

The invention discloses an acinetobacter strain and application thereof in improvement of plant stress resistance. The invention provides Acinetobacter sp. YJ-AD2, and the registration number of the Acinetobacter sp. YJ-AD2 in the General Microbiological Center of the China Committee for Culture Collection of Microorganisms is CGMCC No.23604. The Acinetobacter sp. YJ-AD2 can be applied to the field of biological engineering. The Acinetobacter sp. YJ-AD2 provided by the invention can improve the saline-alkali stress resistance of rice, corn and arabidopsis thaliana, specifically, the dry weight, the fresh weight and the total root length are obviously increased, the height of the overground part is increased (i.e., the plant height is increased), the leaf length is increased, the total surface area of the leaf is increased, and the leaf wilt is reduced. By detecting the biochemical experiment of the YJ-AD2 as the growth-promoting bacteria, the YJ-AD2 has the capabilities of dissolving phosphorus, fixing nitrogen, secreting auxin and secreting ACC deaminase, and the indexes are important evidence that the YJ-AD2 has the potential of the growth-promoting bacteria. The method has an important application value.

Description

technical field [0001] The invention relates to the field of biological agriculture, in particular to a strain of Acinetobacter and its application in improving plant stress resistance. Background technique [0002] Plants growing in natural environments are often seriously threatened by various biotic and abiotic stresses. Salt stress is one of the main abiotic stresses threatening sustainable crop production worldwide. Saline-alkali land affects the normal growth of plants and reduces the yield and biomass of plants. For agriculture, the salinization of land poses a threat to food security. Land salinization is currently an important bottleneck restricting agricultural development and ecological governance in my country. Human activities coupled with rapid climate change are the main driving factors of soil salinization that lead to agricultural land degradation. Excessively high salt concentrations lead to soil degradation, altering soil infiltration and matrix potenti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P17/10C12N9/78C12N11/04C12N11/08C12N11/084C12N11/10C05G3/00C05G3/60C05G3/80A01G7/06A01N63/20A01P21/00C12R1/01
CPCC12N1/20C12P17/10C12N9/78C12Y305/99007C12N11/04C12N11/10C12N11/084C12N11/08C05G3/00A01G7/06A01N63/20
Inventor 李立新黄咏雪宋扬
Owner NORTHEAST FORESTRY UNIVERSITY
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