1108results about "On/in organic carrier" patented technology

The invention discloses a rapid restoration method for degraded grassland based on a microbial agent. The microbial agent is formed by compounding 15-30 parts of a nitrogen-fixing microbial agent, 10-20 parts of a phosphate-solubilizing microbial agent, 8-15 parts of a plant growth-promoting microbial agent, 25-40 parts of a humic acid carrier, 3-8 parts of an embedding agent sodium alginate and 5-12 parts of a nutritional agent molasses. Wherein the nitrogen-fixing bacteria are azotobacter chroococcum and alfalfa rhizobium (the viable count is greater than or equal to 5 * 10 < 9 > CFU / g), the phosphate solubilizing bacteria are bacillus megatherium (the viable count is greater than or equal to 1 * 10 < 10 > CFU / g), and the growth-promoting bacteria are bacillus subtilis and pseudomonas fluorescens (the viable count is greater than or equal to 2 * 10 < 10 > CFU / g). The microbial inoculum is subjected to activation, carrier adsorption, sodium alginate embedding granulation and CaCl2 cross-linking processes to be prepared into slow-release particles of 1-3 mm. 200-250 kg of organic fertilizer is applied to each hectare in a mild area, 300-400 kg of organic fertilizer is applied to each hectare in a moderate and severe area, and leymus chinensis and wheatgrass are sown in a mixed mode. After 90 days of application, the vegetation coverage is improved by 40% or above, the soil organic matter is increased by 25-40%, the microbial diversity index is larger than or equal to 3.5, and the comprehensive remediation cost is reduced by 53%.

The invention relates to the technical field of ecological restoration, in particular to a preparation method of novel vegetation concrete greening additive AB bacteria, which comprises the following steps: step (1) screening and activating double bacteria; (2) preparing an inner core structure; (3) loading double bacteria; (4) coating a shell layer; (5) loading a functional matrix; and (6) preparing the vegetation concrete greening additive AB bacteria. According to the method, a multi-layer and function-synergistic microorganism-seed-carrier composite system is constructed and used for surface concrete attachment of the high steep slope, and a plant-microorganism symbiotic system with physical isolation, chemical buffering and nutrition self-sustaining capacities is actively constructed for plant seeds and functional microorganisms in a strongly alkaline macroscopic environment; and finally, the long-acting ecological stability of the high and steep slope is realized.

The invention relates to the technical field of saline-alkali soil treatment, and discloses a light and moderate saline-alkali soil alfalfa seedling protection and strong growth promotion planting method which comprises the following steps: S1, soil pretreatment and root zone microenvironment construction; s2, seed treatment and space-time double-salt-avoiding sowing; s3, microbial inoculum synergistic application; s4, water and fertilizer integration and accurate management; s5, dynamic regulation and control in the growth period, a low-salt root zone microenvironment is constructed through combination of physical obstruction and biological improvement, salt ascending is inhibited by using a slag salt isolation layer, fertility is improved by using an organic layer, and stress resistance is enhanced by adopting a salt-tolerant variety, climate window sowing and aerogel embedding fungicide; precise regulation and control are achieved through water and fertilizer integrated drip irrigation, the stability of a root zone is maintained by combining intertillage and directional topdressing, finally, the seedling emergence uniformity and the seedling stage survival rate are remarkably improved, and robust growth of plants in saline-alkali soil is promoted.

The invention relates to the technical field of soil remediation, in particular to a charcoal-fungus symbiont for a freeze-thaw environment and a preparation method and application of the charcoal-fungus symbiont. The preparation method of the charcoal-fungus symbiont comprises the following steps: crushing a biomass raw material, and carrying out pyrolysis to prepare basic charcoal; placing the basic biochar in an iron oxide solution, and carrying out an iron oxide loading reaction to obtain iron oxide biochar; the preparation method comprises the following steps: dipping iron oxide biochar in a chitosan solution for coating modification, then continuously adding a cross-linking agent for cross-linking solidification, and freeze-drying to obtain chitosan-iron oxide composite modified biochar; and immersing the chitosan-iron oxide composite modified biochar into a bacterial suspension of functional microorganisms, oscillating and adsorbing, and freeze-drying to obtain the carbon-bacteria symbiont. The invention aims to improve the heavy metal pollution phenomenon of soil and the soybean root rot disaster in the freezing and thawing cycle environment at the same time.

The invention discloses a method for constructing a multicellular living body material by adopting a droplet bioreactor, which belongs to the crossing field of biological materials and microbial engineering, and comprises the following steps: (1) resuspending different cells in different polymer solutions to prepare degradable or phase-separable hydrogel particles containing cells; (2) dispersing the hydrogel particles in a cross-linkable hydrogel matrix to form a composite hydrogel; and (3) curing the composite hydrogel, and then carrying out degradation treatment to obtain the liquid cavity structure multicellular system bioreactor with spatial partition and dynamic interaction characteristics. According to the invention, ordered distribution and metabolism synergy of different microorganisms in space can be realized, controllable degradability and excellent mass transfer performance are realized, bacterial slow release and metabolite exchange can be maintained, and the method is suitable for long-term stable operation of a continuous flow reactor. The method has wide application prospects in the fields of green biological manufacturing, renewable energy source production, environmental restoration, biomedical engineering and the like.

The invention discloses a sphingomonas sphingomonas, a bacterial agent thereof and application of the sphingomonas sphingomonas sphingomonas sphingomonas sphingomonas sphingomonas sphingomonas sphingomonas sphingomonas sphingomonas sphingomonas sphingomonas sphingomonas sphingomonas sphingomonas sphingomonas sphingomonas sphingomonas sphingomonas sphingomonas sphingomonas sphingomonas sphingomonas sphingomonas sphingomonas sphingomonas sphingomonas sphingomonas The sphingomonas disclosed by the invention has excellent saline-alkaline tolerance, has the functions of fixing nitrogen, decomposing organic phosphorus and producing ACC deaminase, breaks through the limitation of single function of a traditional strain, and has a very good application prospect in saline-alkaline soil improvement, plant growth promotion and ecological restoration.

The invention discloses a bacillus coagulans feed additive and a preparation method thereof, and relates to the technical field of feed additives, the bacillus coagulans feed additive comprises bacillus coagulans spores, the content of the spores is 1.0 * 10 < 8 >-1.0 * 10 < 11 > CFU / g; the protective agent system comprises the following components: maltodextrin or lactose, vegetable protein, polyhydric alcohol and an antioxidant; the invention discloses a microcapsule coating structure. A microcapsule is a two-layer composite coating of an inner layer alginate matrix and an outer layer polycation or polymer coating. According to the invention, bacillus coagulans spores which can still keep high survival rate under short-time high-temperature and low-pH conditions are obtained through screening and fermentation strategies of strains with high sporulation and heat and acid tolerance, the problem that common strains are easy to inactivate in granulation, extrusion and gastric acid environments is solved, the problem of product failure caused by insufficient tolerance of the strains is reduced, and the product quality is improved. And the original biological activity basis is improved, so that the protection effect of the subsequent formula and the coating can be played at a higher starting point, and the zoology effect of the final preparation is improved.

The invention discloses a concrete crack self-repairing material based on microorganism immobilization and a preparation method of the concrete crack self-repairing material, and belongs to the crossing field of microorganism materials and civil engineering. The material is prepared from peptone, sodium lactate, volcanic rock, bacillus pasteurii and other raw materials through the steps of culture medium preparation, carrier modification, microorganism immobilization, microcapsule and gel protection layer formation and the like, the prepared volcanic rock-chitosan carrier provides an inhabiting environment for microorganisms, and the survival rate of the microorganisms in a high-alkali environment is increased to 70%. When the concrete cracks and the alkaline environment triggers the protective layer to disintegrate, the bacillus pasteurii and the desulfurization vibrio are subjected to a reduction reaction with sulfate through urealysis, and calcium carbonate and calcium sulfide are generated to fill cracks. The 28-day repair rate of the material is larger than or equal to 99%, the maximum crack repair width reaches 1.2 mm, the compressive strength recovery rate is larger than or equal to 85%, excellent durability and environment friendliness are achieved, and the concrete crack repair problem is effectively solved.

The invention relates to a lake sediment reduction bacterium which is classified and named as bacillus amyloliquefaciens WP02, the preservation number is CCTCC (China Center for Type Culture Collection) NO: M2020696, and the lake sediment reduction bacterium is preserved in the China Center for Type Culture Collection. The invention relates to an application of a lake sediment reduction bacterium in sediment denitrification, phosphorus uptake and quality degradation. The method has the beneficial effects that the immobilized microbial agent obtained by respectively embedding and adsorbing lake sediment reduction bacteria can effectively reduce sediment organic matters, reduce sediment total nitrogen and absorb phosphorus in overlying water, namely effectively perform denitrification, phosphorus absorption and quality degradation on the sediment, and the microbial agent can improve the oxidation reduction potential of the sediment and reduce the pollution of the sediment. And the method has important significance and application value in the aspect of maintaining the ecological balance of the water body, and has the characteristics of high in-situ remediation efficiency, small environmental disturbance and no secondary pollution compared with a physical and chemical remediation method.

The invention relates to the technical field of agricultural microorganisms. The invention provides a compound microorganism bacterium suspension, a bacterium agent, a tobacco seed coating agent as well as a preparation method and application of the compound microorganism bacterium suspension. The compound microorganism bacterium suspension comprises a rice seed endophytic bacterium spiraceae R3 bacterium suspension and a rice seed endophytic bacterium stenotrophomonas R5 bacterium suspension; the mass ratio of R3 to R5 is 1: (0.5-1.5); the endophytic bacterium herbapiril R3 of the rice seeds is Herbapiril sp. R3, and the preservation number of the herbapiril R3 is CCTCC (China Center For Type Culture Collection) NO: M 20231757; the rice seed endophytic bacterium stenotrophomonas R5 is Shenotrophomonas sp. R5, and the preservation number of the rice seed endophytic bacterium stenotrophomonas R5 is CCTCC (China Center for Type Culture Collection) NO: M 20231758. The compound microbial suspension is used as a seed coating agent to coat tobacco seeds, and growth of tobacco root systems can be remarkably promoted.

The invention discloses an enzyme immobilization method based on charge directed crosslinking, and belongs to the technical field of enzyme immobilization. The method sequentially comprises the following steps: reacting an enzyme with a modifier containing an anionic group to generate an electronegative modified enzyme; dissolving a protonated amino carrier in an acidic buffer solution to form a first solution, and dispersing a dissociation anion group-containing carrier and a modification enzyme in an alkaline buffer solution to form a second solution; mixing the two solutions, and constructing a gel network immobilized enzyme through electrostatic crosslinking; and finally, strengthening the gel network by using a multivalent metal ion solution. Directional anchoring of the enzyme in the gel is achieved through charge matching, the network stability and the enzyme microenvironment are synchronously optimized in combination with metal ion coordination, use of a covalent cross-linking agent is avoided in the whole process, conformation damage of an enzyme active center is avoided, the activity retention rate, the operation half-life period and the substrate mass transfer efficiency of the immobilized enzyme are remarkably improved, and the immobilized enzyme has good application prospects. The method is suitable for efficient immobilization of industrial enzymes such as beta-glucosidase, and has high biocompatibility and industrial potential.

The invention relates to the technical field of agriculture, provides a red soil hardening reduction conditioner based on modified straw adsorption carbon as well as a preparation method and application thereof, and solves the problems that an existing red soil hardening conditioner is poor in soil improvement effect and does not have a slow release function. Comprising the following steps: (1) preparing oyster shell-biochar composite powder; (2) loading functional components; (3) directional immobilization of microorganisms; (4) coating a slow-release layer; (5) activating treatment; the red soil hardening reduction conditioner is prepared from the following raw materials in parts by weight: 80 to 100 parts of oyster shell powder, 100 to 200 parts of modified straw adsorption carbon, 10 to 30 parts of rice hull powder, 30 to 50 parts of nano dihydrate gypsum, 100 to 200 parts of a polyglutamic acid solution, 10 to 50 parts of acid-resistant bacillus, 15 to 30 parts of sodium alginate, 30 to 60 parts of humic acid, 10 to 30 parts of carbide slag and 30 to 60 parts of attapulgite.

The invention belongs to the technical field of coastal saline-alkali soil remediation, and particularly relates to a composite microbial agent for coastal saline-alkali soil remediation. The microbial agent is in a granule form, and comprises a carrier, which is a mixture of straw powder and duck manure; the active flora comprises bacillus licheniformis, bacillus megaterium, brevibacillus laterosporus, trichoderma harzianum spore powder, paenibacillus polymyxa, halomonas and pseudomonas fluorescens, the active flora is adsorbed in the carrier, and the halomonas and the pseudomonas fluorescens are subjected to embedding treatment; and the spraying film is sprayed on the surface of the carrier. Through the synergistic effect of multiple strains, the improvement effect on saline-alkali soil and the growth promoting effect on crop growth are improved, and the defect that the function of a single strain is limited is overcome.

The invention belongs to the technical field of environmental protection of bioremediation wastewater, and discloses an immobilized adsorption microsphere, which is prepared from Bacillus spp., Candida utilis and European nitrosomonas through an immobilized embedding technology. The microspheres provided by the invention can effectively repair glutamic acid high ammonia nitrogen fermentation wastewater.

The invention provides a preparation method and application of a bacterium-enzyme-magnetic nano-cluster composite detoxicating agent, the detoxicating agent takes a Fe3O4-HAP nano-cluster as a magnetic core, the surface of the detoxicating agent is subjected to functional modification, a composite flora composed of bacillus amyloliquefaciens, lactobacillus plantarum and saccharomyces cerevisiae is loaded to form sclerotia, a chitosan shell layer is constructed on the outermost layer, and the bacterium-enzyme-magnetic nano-cluster composite detoxicating agent is prepared. And a compound enzyme consisting of aflatoxin Bdegrading enzyme, zearalenone hydrolase and deoxynivalenol invertase is immobilized. The carrier can efficiently and synchronously degrade aflatoxin, vomitoxin and zearalenone, and can realize rapid separation and recovery through an external magnetic field.

The invention belongs to the technical field of soil organic matter improvers, and discloses a soil organic matter improver based on a compound microbial agent and a preparation method thereof, and the soil organic matter improver comprises the following components: a compound enzyme, a modified chitosan microsphere complex, a modified polyelectrolyte, an inhibitor L-methionine sulfoximine, a substrate, an auxiliary factor and the compound microbial agent. A compound enzyme system is used as a core, soil ammonium ions are circularly and efficiently assimilated through glutamine synthetase / glutamate synthetase, and the soil ammonium ions are converted into organic nitrogen; and energy guarantee is provided by virtue of polyphosphate kinase. The modified chitosan microsphere complex is used for protecting and slowly releasing enzyme and a substrate, and controlled release of the inhibitor L-methionine sulfoximine is realized through the modified polyelectrolyte, so that carbon-nitrogen balance of soil is regulated and controlled. The system has a synergistic effect with a compound microbial agent, the problem of soil organic matter reduction caused by excessive nitrogen application is effectively solved, meanwhile, the crop root biomass is remarkably increased, and the soil organic matter content is increased.

The invention discloses heavy metal resistant bacillus velezensis as well as a microbial remediation agent and application thereof. Bacillus velezensis DBS01 is screened from heavy metal contaminated soil in Dabaoshan mining areas in Shaoguan City, Guangdong province, is preserved in China Center for Type Culture Collection (CCTCC), has a preservation number of CCTCC M 20252228, not only has resistance to various heavy metals, but also can metabolize and secrete metabolites such as organic acid, cellulase and growth hormone in different growth stages, and can be used for preventing and treating various heavy metals. Multiple functions of curing heavy metals, dissolving phosphorus and potassium, promoting plant growth and the like are realized. According to the method, bacillus velezensis DBS01 is inoculated into a culture medium containing straw, calcium superphosphate, agar and sodium alginate are added, and a mixture containing the bacillus velezensis DBS01 is freeze-dried, so that the heavy metal contaminated soil in the mining area can be efficiently repaired in an environment-friendly manner at low cost, and the ecological function recovery of the soil is effectively promoted.

The invention belongs to the technical field of sewage treatment, and particularly relates to a preparation process of ferric salt-chitosan composite modified microorganism high-adaptability biochar. Through combination of the ferric salt-chitosan composite modified biochar carrier and the arsenic-antimony resistance functional flora, the composite modified biochar not only retains an excellent pore structure and specific surface area, but also forms a micro-ecological system with nutrition slow release and microenvironment buffering through a synergistic effect among materials; the immobilized functional flora further degrades or converts As and Sb forms through biotransformation, so that synergistic interaction of multiple mechanisms is realized, and the removal efficiency of arsenic-antimony combined pollution is greatly improved. The raw material biochar is wide in source and low in cost, the chitosan and the ferric salt are materials with good environmental compatibility, the whole preparation process is green and environmentally friendly, and the prepared biochar can be suitable for treatment of arsenic and antimony polluted wastewater with high concentration and complex components and has good application value.

The invention provides a preparation method of microalgae gel balls for water treatment, and relates to the field of water treatment, and the preparation method comprises the following steps: step S1, preparing a composite suspension, step S2, carrying out primary crosslinking to form balls, step S3, carrying out secondary crosslinking, step S4, carrying out in-situ enhancement, and step S5, coating. According to the method, microalgae is subjected to compound modification to obtain gel beads, and the finished product gel beads are obtained through coating, so that the swelling resistance and mechanical strength of the gel beads are improved, and the situation that a compact gel network and a multi-layer coating structure hinder nutrient substances, carbon dioxide and the like in a water body from diffusing to microalgae cells is avoided; the photosynthesis efficiency and pollution degradation capability of the microalgae are ensured, and the water treatment efficiency of the microalgae is improved.

The invention belongs to the technical field of walnut protein preparation, and particularly relates to walnut protein powder with high digestibility, low sensitization and low bitterness as well as a preparation method and application of the walnut protein powder. According to the preparation method of the walnut protein powder with high digestibility, low sensitization and low bitterness provided by the invention, proline specific endo protease, recombinant trypsin and carboxypeptidase B are cooperatively used, so that accurate damage to an anti-digestion structure and a sensitization epitope of walnut protein can be realized; according to the method, ultrahigh digestibility (greater than or equal to 92%), thorough desensitization (greater than 99.8%), bitterness inhibition and functional peptide enrichment are synchronously realized, so that the defects of low digestibility, sensitization residue, obvious bitterness, low yield of functional peptide, complex process and the like caused by uncontrollable cutting in the existing random enzymolysis technology are overcome, and meanwhile, the core technical bottleneck of high-value utilization of walnut protein is overcome.

The invention provides a directional enrichment method of soil active organic carbon, a biochar-microorganism composite preparation and a preparation method, and relates to the technical field of soil improvement and agricultural sustainable development. The method comprises the following steps: (1) screening biochar; (2) screening and culturing a functional microbiome which at least comprises carbon-decomposing bacteria, nitrogen-fixing bacteria and phosphate-solubilizing bacteria, and compounding the carbon-decomposing bacteria, the nitrogen-fixing bacteria and the phosphate-solubilizing bacteria according to a CFU ratio of (1-5): (1-3): (1-2); (3) compounding the biochar in the step (1) and the functional microbiome in the step (2) by adopting an adsorption-embedding combined process to form a biochar-microbiome synergistic regulation system; and (4) applying the charcoal-microbiome coordinated regulation system obtained in the step (3) to target soil, and culturing. According to the invention, not only is input of soil organic carbon increased, but also accurate and efficient enrichment of the soil active organic carbon library is realized.

Provided is a composition for controlling uptake of microparticles into a body, the composition containing a lactic acid bacterium. The lactic acid bacterium is preferably any one selected from bacteria belonging to the genus Lactobacillus and bacteria belonging to the genus Streptococcus. For example, the lactic acid bacterium is a bacterium belonging to Lactobacillus delbrueckii, Lactobacillus paracasei, Lactobacillus gasseri, or Streptococcus thermophilus.

The invention relates to a DNA framework structure addressable enzyme ligation method. Specifically, the invention provides a method for accurately regulating and controlling the specific position of an enzyme on a DNA frame structure through an enzyme modification and base complementary pairing principle, and provides a frame nucleic acid with a capture segment and an enzyme with a connecting chain, and the frame nucleic acid with the capture segment and the enzyme with the connecting chain are subjected to base complementary pairing through the connecting chain and the capture segment. Therefore, the enzyme is connected to a predetermined binding site of the framework nucleic acid, and accurate regulation and control of the connection position, number spacing and the like of the enzyme on the framework nucleic acid are realized. The connection method provided by the invention can realize addressable and accurate enzyme position regulation and control on the DNA nano structure, can widen site-specific modification of the enzyme on the DNA frame structure, and has important significance for developing site-specific and high-throughput enzymatic reaction.

The invention relates to the technical field of microbial fermentation, and discloses an agricultural ecological probiotic complex microbial inoculant, which is prepared from the following components in percentage by mass: 30 to 35 weight percent of beneficial microbial inoculant, 20 to 25 weight percent of mineral source potassium fulvate, 15 to 20 weight percent of slow release agent, 10 to 15 weight percent of nano polypeptide fermentation liquor and the balance of carrier, the processing method comprises the following steps: S1, pretreating the raw materials; s2, preparing nano polypeptide fermentation liquor; s3, complex microbial inoculants are compounded; and S4, drying and forming. According to the invention, the mineral source potassium fulvic acid and the nano-polypeptide fermentation liquor are added, so that effective regulation and control of soil micro-ecology are realized; crop growth is promoted through cooperation of multiple strains, growth hormone is secreted through bacillus subtilis and the like, nitrogen is fixed through bacillus amyloliquefaciens, root development is stimulated in cooperation with mineral source potassium fulvic acid, and the plant height, stem diameter and fresh weight of crops are remarkably increased; the particle size of the polypeptide subjected to high-pressure homogenization treatment is 80-120nm, the absorption efficiency is improved by 60%, and a crop disease-resistant signal channel is activated.

The invention belongs to the technical field of environmental engineering, and discloses a microbial agent for degrading malathion as well as a preparation method and application of the microbial agent. The microbial agent comprises the following raw materials: compound bacteria, a nutritional supplement and a biomass carrier material, wherein the compound bacteria comprise functional bacteria and synergistic bacteria, and the mass ratio of the functional bacteria to the synergistic bacteria is (1.2-1.8): 1; the functional bacteria comprise issatchenkia orientalis, nocardia, brevibacillus laterosporus and bacillus cereus; the synergistic bacteria comprise azospirillum brasilense, bacillus thuringiensis and lactobacillus buchneri. Functional bacteria with direct degradation capacity and synergistic bacteria with auxiliary functions are creatively compounded according to a specific proportion, meanwhile, the garlic straw powder is selected as a biomass carrier material, all the raw materials complement each other in function and have a synergistic effect, the degradation rate and thoroughness of malathion are remarkably improved, and the degradation efficiency of malathion is greatly improved. The defects of low degradation efficiency and poor environmental adaptability of a single strain are overcome.

The invention relates to the technical field of dairy product fermentation, and discloses a high-activity probiotic microcapsule suitable for dairy product fermentation and a preparation method thereof.The microcapsule is prepared from, by weight, 6.7-13.3 parts of bifidobacterium lactis, 3.3-6.7 parts of lactobacillus plantarum, 9.1-15.2 parts of Arabic gum, 13.6-22.7 parts of maltodextrin, 7.3-12.1 parts of chitosan, 15.1-30.2 parts of trehalose, 3.8-7.5 parts of glycerinum and 1.1-2.3 parts of ascorbic acid. The molecular weight of the chitosan is 80kDa, and the weight part ratio of the bifidobacterium lactis to the lactobacillus plantarum is 2: 1. In the invention, an inner core is formed through a eutectic structure of bifidobacterium lactis, lactobacillus plantarum and trehalose to protect the probiotics, so that the problems that the existing probiotics are easily influenced by the external environment in the storage and fermentation processes, the activity is reduced, and the fermentation effect of dairy products is poor are solved.

The invention relates to a composite microbial fertilizer with a saline-alkali soil improvement effect and a preparation method thereof, and belongs to the technical field of microbial fertilizer preparation, the fertilizer comprises the following raw materials by mass: 10-30 parts of modified charcoal, 5-8 parts of a microbial agent, 30-50 parts of an inorganic fertilizer, 10-20 parts of a soil improvement agent and 3-5 parts of other auxiliary agents; wherein the modified biochar is obtained by performing segmented heat treatment modification on animal husbandry wastes; the microbial agent is obtained by immobilizing compound microorganisms by using sodium alginate gel reinforced by a mineral filler and micron-sized charcoal powder. The prepared fertilizer has the effects of improving crop quality and crop yield and improving saline-alkali soil.

Disclosed in the present invention are a biomimetic vascularized iPSC-hep spheroid for liver regeneration, and a preparation method therefor. The biomimetic vascularized iPSC-hep spheroid is a microcapsule with a porous hyaluronic acid methacryloyl as a shell and a human-induced pluripotent stem-cell-derived hepatocyte spheroid as an inner phase, and human umbilical vein endothelial cells adhere to the surface of the shell. The biomimetic vascularized iPSC-hep spheroid of the present invention promotes effective communication between hiPSC-heps and HUVECs, and provides barrier protection for an encapsulated hiPSC-hep spheroid so as to avoid attack by the immune system. Compared with dispersed cells, the cell-to-cell interaction in the cell spheroid improves cell-to-cell communication, helps to simulate the physiological heterogeneous structure and cell microenvironment of the liver, and can more effectively repair an acutely failing liver.

The invention discloses a single-cell probiotic packaging method of thiolated guluronic acid oligosaccharide and a probiotic microcapsule, and relates to the technical field of biological medicines. The packaging method comprises the following steps: mixing a thiolated guluronic acid oligosaccharide solution with a probiotic suspension to form a probiotic-polysaccharide mixed solution; and adding a calcium chloride solution into the probiotic-polysaccharide mixed solution, and stirring to form the single-cell microcapsule encapsulating the probiotics. The packaging method can protect a single strain from being damaged by an external adverse environment to the maximum extent, and the survival rate is increased. Meanwhile, the obtained single-cell microcapsule can improve the digestion of the probiotics, the survival rate of the probiotics under the oxidative stress environment and the antibiotic combination, has high stability, and prolongs the intestinal residence time of the probiotics. An innovative solution is provided for the problems that in the existing medicine field, probiotic preparations cannot improve the gastrointestinal stress resistance of probiotics, and antibiotics lose efficacy when combined.

The invention discloses a microbial capsule as well as a preparation method and application thereof, and belongs to the technical field of microbial capsule preparation. The microbial capsule comprises a core material and a capsule wall coated outside the core material, the core material consists of microorganisms and a microorganism culture solution; the microorganism is composed of bacillus pasteurii (S.pasteurii), psychrobacter sp. (Psychrobacter sp.) and phosphate solubilizing bacteria, and the microorganism is prepared from bacillus pasteurii, psychrobacter sp. (Psychrobacter sp.) and phosphate solubilizing bacteria. Through microbial encapsulation, the problem that microorganisms are difficult to survive in a low-temperature environment is successfully solved, construction of a cooling capacity trap system is facilitated, and cooling capacity loss is reduced. Through microbial compounding, the MICP technology is suitable for a low-temperature environment and an extremely low-temperature environment. The capsule shell can improve protection, so that the microorganisms keep activity in a low-temperature environment, and low-temperature inactivation of the microorganisms is avoided; the situation that microorganisms run off along with fluid can be reduced, the adhesion capacity of the microorganisms in a target area is enhanced, and the microorganism thallus colonization rate is increased.