Patents
Literature
22 results about "Transposase" patented technology
Efficacy Topic
Property
Owner
Technical Advancement
Application Domain
Technology Topic
Technology Field Word
Patent Country/Region
Patent Type
Patent Status
Application Year
Inventor
Transposase is an enzyme that binds to the end of a transposon and catalyzes its movement to another part of the genome by a cut and paste mechanism or a replicative transposition mechanism. The word "transposase" was first coined by the individuals who cloned the enzyme required for transposition of the Tn3 transposon. The existence of transposons was postulated in the late 1940s by Barbara McClintock, who was studying the inheritance of maize, but the actual molecular basis for transposition was described by later groups. McClintock discovered that pieces of the chromosomes changed their position, jumping from one chromosome to another. The repositioning of these transposons (which coded for color) allowed other genes for pigment to be expressed. Transposition in maize causes changes in color; however, in other organisms, such as bacteria, it can cause antibiotic resistance. Transposition is also important in creating genetic diversity within species and adaptability to changing living conditions. During the course of human evolution, as much as 40% of the human genome has moved around via methods such as transposition of transposons.
Combined sequencing method of single-cell chromatin accessibility and transcriptome
PendingCN122459470ACell stainingDroplet microfluidics
Provided are a single-cell chromatin accessibility and transcriptome combined sequencing method and application thereof. The method comprises: treating a cell nucleus using a transposase to obtain gDNA of a chromatin open region connected with a first specific tag sequence; treating the cell nucleus using a transcriptome capture sequence to obtain cDNA connected with a second specific tag sequence; generating a droplet based on a droplet microfluidic to seal the cell nucleus and a first microbead in the droplet, wherein the gDNA and the cDNA are captured by the first microbead, and wherein more than one cell nucleus is sealed in the droplet; and sequencing the gDNA and the cDNA to obtain combined information of single-cell chromatin accessibility and transcriptome according to the first specific tag sequence and the second specific tag.
Owner:SHENZHEN HUADA GENE INST
A high-temperature-resistant acetobacter pasteurii genetically engineered bacterium, a construction method and application thereof
PendingCN122256213Aimprove survival rateIncrease synthesisBacteriaMicroorganism based processesBiotechnologyAcetobacter
The application belongs to the technical field of bioengineering, and discloses a gene engineering bacterium of acetobacter pasteurii with high temperature resistance, a construction method and application thereof. Acetobacter pasteurianus The gene engineering bacterium is constructed by using the acetobacter pasteurii (ATCC 10137) as a starting strain and knocking out the transposase gene in the genome of the acetobacter pasteurii (ATCC 10137) by a homologous recombination method. TnsR The gene engineering bacterium with high temperature resistance is obtained. TnsR The deletion of the gene is beneficial to maintaining the stability of the genome, so that the strain can maintain better growth and metabolic capacity under high-temperature pressure. Experimental results show that the biomass, survival rate and acetic acid yield of the gene engineering strain under the condition of 40 DEG C high temperature are significantly better than those of the wild-type strain. When the gene engineering strain is applied to traditional solid-state food vinegar brewing, the yield of organic acids such as acetic acid is significantly improved, the content of ester flavor substances is greatly improved, and the flavor quality of food vinegar is effectively improved. The application provides an effective technical means for solving the problem that high temperature in summer leads to abnormal fermentation and yield reduction of food vinegar.
Owner:JIANGSU UNIV OF SCI & TECH
A base editor, a base editing method and application thereof
This invention discloses a base editor, a base editing method, and its applications. First, the invention constructs highly efficient targeting sites unrestricted by PAM sequences through point mutation. A random insertion library is constructed using a transposase system, and proteins capable of cytosine base editing in mammals are screened using a BFP-GFP reporter system. Further point mutations are performed on deaminases to screen for highly efficient cytosine base editors unrestricted by PAM. The optimized base editing method of this invention achieves an efficiency of over 70%, with some sites reaching over 90%, demonstrating significant application value in basic life sciences, medicine, and other fields.
Owner:SHANGHAI INST FOR BIOMEDICAL & PHARM TECH
Methods and compositions for molecular interaction mapping using transposase
PendingUS20260139307A1Fusion with DNA-binding domainPolypeptide with affinity tagCell stainingCell biology
Compositions, methods, and kits for performing multiplexed, spatially resolved, or single-cell chromatin analysis are provided.
Owner:NEW YORK GENOME CENT
Retrotransposon systems for genome editing and their applications
This application provides a retroposophyte system for editing the genome and its use therein, wherein the retroposophyte in the retroposophyte system comprises an amino acid sequence as shown in any one of SEQ ID NO:1-158, SEQ ID NO:475-489, SEQ ID NO:515-520 and SEQ ID NO:522-530, or comprises an amino acid sequence having at least 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, or 99% identity with any one of SEQ ID NO:1-158, SEQ ID NO:475-489, SEQ ID NO:515-520 and SEQ ID NO:522-530; or the retroposophyte is a truncated version of an amino acid sequence as shown in any one of SEQ ID NO:1-158, SEQ ID NO:475-489, SEQ ID NO:515-520 and SEQ ID NO:522-530. This application also relates to engineered sequences of retrosockase coupled with other amino acid sequences; and engineered sequences of truncated retrosockase coupled with other amino acid sequences.
Owner:BEIJING INST FOR STEM CELL & REGENERATIVE MEDICINE +1
BZ transposase, transposon system and application thereof
PendingCN122071693APeptide/protein ingredientsGenetic material ingredientsAmino acid substitutionWild type
The present invention relates to a transposon system comprising a transposase and a transposon, the transposase is a mutant transposase or a fusion transposase, the mutant transposase has one or more amino acid substitution mutations compared to a wild type ZB transposase as shown in SEQ ID NO: 1, and has improved transposon efficiency or transposon target preference.
Owner:MAXIRNA (SHANGHAI) PHARM CO LTD +2
Compositions and methods for induced stem cell differentiation to oligodendrocytes
A method of differentiating nonhuman primate induced pluripotent stem cells (iPSCs) to oligodendrocytes is provided. The method may include providing a modified vector comprising a piggyBac vector backbone that expresses one or more differentiation factor genes, including SOX10, OLIG2, and NKX6-2. The piggyBac vector backbone may comprise one or more terminal inverted repeats and one or more transposase recognition sites configured to interact with a piggyBac transposase to mediate genomic integration at TTAA or noncanonical target sequences. The vector may further include a selectable marker and a promoter for regulated expression. The modified vector may be introduced into nonhuman primate iPSCs, for example from Macaca fascicularis, by electroporation and used in a feeder-free differentiation workflow. The resulting differentiated cells may be identified by expression of oligodendrocyte-associated markers including O4, O1, PDGFRA, MOG, GALC, CNPase, and myelin basic protein (MBP).
Owner:EXIR LLC
Method for transposase-mediated spatial tagging and analyzing genomic DNA in a biological sample
The present disclosure relates to materials and methods for spatially analyzing nucleic acids that have been fragmented with a transposase enzyme, alone or in combination with other types of analytes.
Owner:10X GENOMICS INC
Method for producing genetically modified cells
To provide a method for producing gene-modified cells that offers superior efficiency in introducing the target gene into target cells, excellent survival rates of target cells into which the target gene has been introduced, and long-term expression of the target gene. [Solution] A method for producing genetically modified cells, comprising: contacting target cells with a vector containing a target gene sequence; contacting target cells with mRNA containing a sequence encoding a transposase for introducing the target gene sequence into the genomic DNA of the target cells; irradiating the target cells with plasma; and obtaining genetically modified cells into which the vector and mRNA have been introduced.
Owner:SEKISUI CHEMICAL CO LTD
Method for detecting single-strand breaks in nucleic acids
The present invention relates to a method for detecting single-strand breaks (SSBs) in double-stranded (ds) DNA. A sample of dsDNA is brought into contact with an oligonucleotide probe containing a first sequencing adapter and tag, and then into contact with a DNA ligase. The DNA ligase covalently binds the oligonucleotide probe to the dsDNA strand at the single-strand break sites in the dsDNA. The dsDNA sample is then brought into contact with a transposase loaded with a second sequencing adapter, resulting in the cleavage of the dsDNA by the transposase and the generation of a population of DNA fragments containing the second sequencing adapter. DNA strands containing the first and second sequencing adapters are isolated from the population, and the sequences of the isolated DNA strands are determined. A method and a kit for carrying out the method are provided.
Owner:アルトス ラブズ インコーポレイテッド
An antibody reprogramming and screening method based on engineered cells and application thereof
PendingCN122235138ADifficult to break through and difficult to cultivateBreaking through limitations such as difficulty in cultivation and difficulty in operationAntibody ingredientsImmunoglobulins against cell receptors/antigens/surface-determinantsFunctional identificationRecombination signal sequences
This invention relates to a method for antibody reprogramming and screening based on engineered cells and its applications. This method achieves efficient in vitro rearrangement and diversity generation of antibody genes in non-lymphocytes by constructing engineered human cells containing the RAG1 / 2 recombinase system and programmable recombination signal sequence (RSS) elements. Utilizing this invention, the V(D)J rearrangement mechanism of B cells can be simulated in engineered cells, allowing for the targeted introduction of insertions, deletions, and point mutations in the antibody complementarity-determining region (CDR), resulting in a structurally diverse antibody library. Combined with piggyBac transposase-mediated genome integration, mammalian cell surface display technology, and flow cytometry sorting, high-throughput antibody screening and functional identification are achieved. This invention is simple to operate, has high antibody gene rearrangement efficiency, and produces a rich diversity of antibody libraries, making it suitable for rapid optimization of antibody drugs and discovery of new antibodies.
Owner:SHANGHAI JIAOTONG UNIV
A transposase library building method that reduces linker dimer in a library
This invention provides a transposase library construction method and related products for reducing adapter dimers in libraries, belonging to the field of biotechnology. By optimizing the primers and polymerases used in the transposase library construction process, this invention effectively reduces the proportion of adapter dimers in libraries with low input levels, thereby improving sequencing quality.
Owner:NANJING VAZYME BIOTECH CO LTD
Inducible Tn5 transposon shuttle plasmid and its preparation method and application
Owner:SHANTOU UNIV
Self-inactivating transposase plasmids and uses thereof
Some embodiments provided herein relate to gene delivery systems and methods using a single plasmid that carries a self-inactivating transposase gene and a corresponding transposon. Some embodiments include nucleic acids having certain sequences, vector including such nucleic acids, and compositions including the vectors.
Owner:SEATTLE CHILDRENS HOSPITAL (DBA SEATTLE CHILDRENS RES INST)
Hyperactive transposons and transposases
The present invention relates to a polypeptide comprising a piggyBac transposase or a fragment or a derivative thereof having transposase function comprising at least one amino acid substitution. Further, the present invention relates to a transposable element comprising a piggyBac or piggyBac-like left repeat sequence and left internal repeat sequence, wherein the left internal repeat sequence comprises at least one nucleotide modification. Furthermore, the present invention relates to a kit comprising the above transposase and / or transposable element. In addition, the present invention relates to a targeting system comprising the above transposase and / or transposable element.
Owner:PROBIOGEN AG
Truncated transposase, transposome system and applications thereof
The application provides a truncated transposase, a transposon system and application thereof. Compared with a wild-type PS transposase, the truncated transposase lacks 1-50 amino acids at the C terminal of the wild-type PS transposase, preferably 44 amino acids. By deleting the amino acids at the C terminal of the transposase which have less influence on transposition, the length of the transposase is shortened, which is beneficial to the expression of the transposase and the preparation of a fusion protein, so that a transposase or a fusion protein thereof with higher catalytic activity and smaller size is obtained. The application also provides a fusion protein of the transposase, which is preferably fused with a leucine zipper through a linker, and a transposon system comprising the transposase and the transposon, which has great application value in the transgenic industry.
Owner:MAXIRNA (SHANGHAI) PHARM CO LTD +2
Escherichia coli Rosetta strain and its application in the catalytic synthesis of α-arbutin
ActiveCN116162640BHigh catalytic efficiencyhigh speedBacteriaMutant preparationGenetic enhancementSucrose phosphorylase
This invention discloses an *E. coli* Rosetta strain for the biosynthesis of α-arbutin. Recombinant genes for FruA, CscK, Pgi, and sucrose phosphorylase SmsP are inserted into the genome of the *E. coli* Rosetta strain, anchoring the sucrose phosphorylase SmsP protein product to the surface of *E. coli* cells. The invention also discloses the corresponding transposase plasmids and CRISPR plasmids, as well as the application of the strain in the catalytic synthesis of α-arbutin. This invention utilizes *E. coli* Rosetta (DE3) as the chassis cell and uses CRISPR transposition technology to enhance the FruA, CscK, and Pgi genes in the fructose metabolic pathway, thereby enhancing the metabolic pathway of the reaction's accompanying product, fructose. Enzyme anchoring technology is used to anchor sucrose phosphorylase to the surface of *E. coli*, allowing the sucrose phosphorylase to grow on the *E. coli* surface and catalyze the conversion of sucrose and hydroquinone from the external environment into α-arbutin and fructose.
Owner:TIDETRON BIOWORKS TECH (GUANGZHOU) CO LTD +1
Compositions, methods, and systems for DNA modification
Provided herein are compositions, methods, and systems for DNA modification. In particular, provided herein are compositions, and systems comprising TnpB-like nuclease-dead repressors (dTnpB / TldRs), dCas12f or dCas12f-like proteins, and / or a TnpB-transposase fusion proteins and methods using thereof.
Owner:THE TRUSTEES OF COLUMBIA UNIV IN THE CITY OF NEW YORK
Methods and compositions for solid capture-based spatial cut&tag
Compositions, methods, and kits for performing multiplexed, spatially-resolved chromatin analysis. In certain embodiments, a transposome complex comprising a first transposase and a second transposase are provided, the first transposase having a first adapter comprising a T7 promoter and the second transposase having a second adapter comprising a capture sequence, the first transposase and the second transposase each being a fusion protein comprising an antibody-binding ligand.
Owner:NEW YORK GENOME CENT +1
Targeted gene editing constructs and methods of using them
This disclosure provides nucleic acid constructs for use in enhancing the site-specific insertion of an exogenous nucleic acid into a genome. In some embodiments, the nucleic acid construct comprises a first polynucleotide sequence encoding a DNA-binding protein engineered to bind to a specific genomic DNA sequence, a second polynucleotide comprising a modified integrase or a modified transposase that enables insertion of the exogenous nucleic acid into the genome, and a nucleic acid sequence encoding a linker between the two nucleotides. In some embodiments, the nucleic acid construct encodes a fusion protein, for example, a fusion protein for delivery to a cell via a lentiviral particle.
Owner:UNIV POMPEU FABRA