The invention relates to a method for modifying an
animal genome by
directed mutagenesis, in particular relates to a novel directional transformation method of a specific
gene of an
animal genome by utilizing
zinc finger
nuclease. The method comprises the following steps: designing the
zinc finger
nuclease which specifically identifies and cuts a targeting
gene according to a sequence in a target animal
target gene region; validating the directional
cutting capability and efficiency of the
zinc finger
nuclease to the
target gene by using an independent expression
system; and carrying out directional genetic modification on the
target gene of the
embryo of a target animal to obtain a stable genetic character by utilizing the selected
zinc finger nuclease. Furthermore, the invention also relates to a main
gene mutation mode which is obtained by using the independent expression
system, selecting and utilizing the
zinc finger nuclease, and a filial generation obtained through efficient screening and oriented genetic modification by utilizing the selected
gene mutation mode. On the other hand, the invention relates to the
zinc finger nuclease which is obtained by utilizing the method and is capable of specifically identifying the
myostatin gene of
pelteobagrus fulvidraco and directionally knocking out the
myostatin gene of the
pelteobagrus fulvidraco.