Construction method of CD155 gene humanized non-human animal and application

A non-human animal and construction method technology, which is applied in the field of CD155 genetically modified non-human animal model construction, can solve the problem that animal models cannot reflect the real situation of the human body well
CN112458117AActive Publication Date: 2021-03-09BIOCYTOGEN PHARMACEUTICALS (BEIJING) CO LTD
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Patent Information

Authority / Receiving Office
CN ยท China
Patent Type
Applications(China)
Current Assignee / Owner
BIOCYTOGEN PHARMACEUTICALS (BEIJING) CO LTD
Publication Date
2021-03-09

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Abstract

The invention provides a construction method of a CD155 gene humanized non-human animal. A nucleotide sequence for encoding human CD155 protein is introduced into a non-human animal genome in a homologous recombination manner; the humanized CD155 protein can be normally expressed in the animal; and the animal can be used as an animal model for human CD155 signal mechanism research and tumour and immune disease drug screening. The important application value is realized on the research and development of new drugs of immune targets. The invention also provides humanized CD155 protein, a humanized CD155 gene, a targeting vector of the CD155 gene, a non-human animal obtained by the construction method, and application of the non-human animal in the field of biological medicines.
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Description

technical field

[0001] The invention belongs to the field of animal genetic engineering and genetic modification, and in particular relates to a method for constructing a CD155 genetically modified non-human animal model and its application in the field of biomedicine. Background technique

[0002] CD155, also known as PVS, HVED, NECL5, TAGE4 or Necl-5, is a Nectin-like type I transmembrane glycoprotein, which belongs to the PVR-related (PRR) family under the immunoglobulin superfamily, and is an adhesion that promotes cell binding Protein, which can bind to poliovirus (PV for short) and allow it to enter susceptible cells, so it is more commonly called PVR (Poliovirus Receptor, poliovirus receptor). CD155 is expressed in monocytes, macrophages, endothelial cells, epithelial cells, CD34+ thymocytes and neurons, but it is highly expressed in most tumor cells, including lung cancer, breast cancer, ovarian cancer and melanoma. Studies have shown that CD155 plays an important r...

Claims

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