148 results about "Blastocyst" patented technology

The invention discloses an image detection and evaluation method for an unfrozen form of a fertilized egg embryo. The method comprises the following steps: acquiring a time sequence image sequence after the fertilized egg embryo is unfrozen; performing image standardization processing on the time sequence image sequence to generate a standardized embryo image set; extracting multi-dimensional embryo quality parameters from the standardized embryo image set; wherein the multi-dimensional embryo quality parameters comprise a morphological static parameter set, a dynamic change parameter set and a texture feature parameter set; generating a blastocyst formation rate based on the multi-dimensional embryo quality parameters; outputting a third-level embryo quality evaluation result according to a comparison relationship between the blastocyst formation rate and a preset threshold interval; wherein the three-stage embryo quality evaluation result comprises an excellent embryo grade, a qualified embryo grade and an abnormal embryo grade. The method has the following advantages and effects: full-automatic and multi-dimensional quantitative evaluation of the thawing process of the unfrozen embryos is realized, so that the scientificity of embryo screening and the success rate of clinical pregnancy are improved.

The present invention provides: a drug which can improve the implantation rate of an embryo and can be used in conjunction with conventional drugs for infertility treatment; and a screening method and the like with which it is possible to reliably evaluate such a drug. Specifically, the integrin activator comprises: (1) a peptide composed of KFEERMRCKWMT, and (2) a peptide composed of KFEERMRCKWMT. (2) a peptide which is composed of KFEEERSRCKWMT; (3) a peptide comprising an amino acid sequence obtained by deleting, substituting and / or adding 1-3 amino acids in (1) or (2); or (4) a peptide obtained by binding any one of (1) to (3) to a membrane-permeable molecule. Provided are an artificial uterus and a screening method based on an implantation reproduction test system using a fertilized egg (for example, an embryo developed to an implanted blastocyst), the artificial uterus comprising: a hydrogel containing endometrial stromal cells and having an extracellular matrix as a main component; and a uterine epithelial organoid configured to be surrounded by the hydrogel.

The invention belongs to the field of antibody development, and discloses a screening method and application of a human-mouse protein high homologous target antibody based on a fully humanized antibody mouse. Aiming at the problem of weak antibody response caused by immune tolerance of human-mouse high homologous targets (protein homology is greater than or equal to 95%), the following scheme is provided: in embryonic stem cells (ES cells) of HUGO-Mabfully humanized antibody transgenic mice, a mouse target gene (such as ACVR2A) is knocked out through a Turbo Knockout technology, and homozygous knockout ES clones are screened; carrying out microinjection on the clones to the whitened B6 mouse blastocyst, and transplanting a pregnant mouse to obtain a Founder mouse; the Founder mouse is subjected to target antigen immunization for more than or equal to 4 times (the Freund's complete adjuvant is used for the first time), and the titer of the serum antibody is detected. According to the invention, 100% homozygous knockout chimeric efficiency is realized in the Founder stage, the mouse construction period is shortened from traditional 8-10 months to 3-4 months, and the diversity and affinity of the antibody are significantly improved (titer reaches 1: 729,000). The obtained antibody can be used for preparing medicines for treating tumors or autoimmune diseases.

The invention provides an improved blastocyst culture solution as well as a preparation method and application thereof, and relates to the field of embryonic cell in-vitro culture. Based on a formula of a conventional blastocyst culture solution, colony stimulating factor-1 (CSF-1) and a transforming growth factor-alpha (TGF-alpha) are added, and the colony stimulating factor-1 and the transforming growth factor-alpha are dissolved in the conventional culture solution according to the concentration of 0.02-0.06 mmol / L and the concentration of 0.01-0.05 mmol / L; the obtained improved blastocyst culture solution can improve the mulberry embryo formation rate or blastocyst formation rate in in-vitro culture, promote the development of low-quality embryos and improve the mulberry embryo quality or blastocyst quality, and can be used for preparing medicines for promoting embryo implantation or subsequent growth and development.

The invention belongs to the field of biomedical image processing, and relates to a blastocyst-stage embryo morphological quality evaluation method based on multi-modal information, which comprises the following steps: acquiring an embryo microscopic image and clinical data of a patient, and acquiring a trained embryo morphological quality evaluation model, based on the trained embryo morphological quality evaluation model, performing knowledge distillation according to the embryo microscopic image and clinical data of the patient to obtain a student model; obtaining an embryo microscopic image and clinical data of a patient, and inputting the embryo microscopic image and the clinical data of the patient into the student model to obtain an evaluation result; the embryo morphological quality evaluation model comprises a multi-modal feature extraction module, a comparison module, a multi-modal feature fusion module and a multi-task embryo quality rating module; according to the method, the comparison learning mechanism is utilized to align the embryology evaluation text information with the image features, so that the evaluation accuracy is improved.

The invention belongs to the technical field of assisted reproduction, and particularly relates to a glucose slow-release structure used in an embryo in-vitro culture solution, a preparation process and a use method. The glucose slow-release structure comprises at least one inner layer containing glucose and a biodegradable material outer layer wrapping the inner layer, and the biodegradable material outer layer comprises a matrix with a plurality of micropores and at least one sealing material layer used for sealing pores of the micropores. According to the invention, gradient release from low sugar in the cleavage stage to high sugar in the blastocyst stage is realized, midway liquid change or artificial sugar supplement is not needed, the operation is obviously simplified, and pollution and mechanical stress risks are reduced.

Described are small molecule cocktails that enable de novo derivation and long-term culture of bovine extraembryonic endoderm cells (bXENs). Methods of using the small molecule cocktails to generate bXENs are also described. Methods of using the bXENs to form blastocyst models or to maintain the stemness of bovine ESCs and prevent them from differentiation are also described.

The invention relates to the technical field of livestock and poultry breeding, in particular to a goat hybridization breeding method capable of achieving complete-cycle nutrition enhancement. The core of the method is that systematic nutrition intervention runs through the whole breeding process. Before hybridization, performing differential nutrition pretreatment on the parents for 60 days: feeding the female parents with a breeding nutrition enhancer added with components such as D-chiro-inositol and N-acetylcysteine, and supplementing sperm motility enhancer containing acetylated L-carnitine and ergothioneine to the male parents. In the gamete treatment stage, the oocytes are subjected to in-vitro maturation culture in a special culture solution containing components such as D-chiro-inositol and a growth differentiation factor 9; after the sperms are subjected to gradient centrifugal screening, completing in-vitro fertilization in an optimized fertilization culture solution; after the embryos develop into blastocysts in the sequential culture system, carrying out laparoscopic transplantation, and carrying out staged precise nutrition culture on the pregnant ewes. According to the method, through multi-level nutrition enhancement, the reproductive capacity of the goats and the growth performance of offspring are effectively improved.

It is revealed that an organ such as pancreas can be regenerated by utilizing a fact that the deficiency of an organ is complemented by injecting an induced pluripotent stem cell (iPS cell) into a developed blastocyst in a blastocyst complementation method. Thus, the present invention has solved the above-described object. This provides a method for producing a target organ, using an iPS cell, in a living body of a non-human mammal having an abnormality associated with a lack of development of the target organ in a development stage, the target organ produced being derived from a different individual mammal that is an individual different from the non-human mammal.

Provided are a reagent combination or a kit for constructing an embryoid and the use thereof. The reagent combination or the kit can be used for inducing a stem cell to produce a blastocyst-like lineage precursor cell, a blastocyst-like cell and / or an embryoid, which are based on small-molecule induction and independent of transgenes, and have a single cell source. The produced blastocyst-like lineage precursor cell and blastocyst-like cell have a full blastocyst lineage, and are seed cells having balanced developmental competence and totipotency, which solves the problem of unbalanced cell maturation in the prior art. The produced embryoid is highly similar to natural embryos in terms of morphological characteristics and transcriptomic characteristics, has the capability of highly reproducing post-implantation embryonic development and / or gastrulation, which is beneficial for research on embryonic development in vitro. The method for inducing the stem cell to produce the blastocyst-like lineage precursor cell, the blastocyst-like cell and / or the embryoid by using the reagent combination or the kit is simple, and has high applicability and high embryoid construction efficiency.

The present invention relates to a medium composition for in vitro fertilization and / or in vitro culture comprising a compound represented by chemical formula 1 or a pharmaceutically acceptable salt thereof, and a method using same. The compound or composition suppresses reactive oxygen species and lipid peroxidation in ovarian granulosa cells to preventing cytotoxicity and mitochondrial dysfunction caused by apoptosis and ferroptosis, leading to an improvement in the quality of aged oocytes, and to increase the developmental rate and blastocyst formation rate of pre-implantation embryos, thereby improving the efficiency of in vitro fertilization. In addition, treatment with the medium composition during the vitrification process of in vitro–fertilized embryos improves re-expansion and survival rates, thereby reducing structural and metabolic damage. Accordingly, the present invention can be advantageously applied to an assisted reproductive technology for treating infertility and subfertility, and in vitro fertilization and / or in vitro culture for improving the propagation efficiency of livestock.

The invention provides an oocyte in-vitro maturation culture solution additive and application thereof. The additive is tert-butylhydroquinone. By adding 100 [mu] g / mL of TBHQ into a basic culture solution, the first polar body discharge rate of oocytes is remarkably increased to 77.29%, the ROS level is reduced, and the blastocyst rate of in-vitro fertilized embryos is increased. The technology is suitable for breed conservation of livestock genetic resources such as sheep and can replace live livestock transportation to reduce the cost.

The invention discloses a blastocyst transplantation feasibility prediction model construction method, system and device, a medium and a product, and relates to the field of result prediction.The method comprises the steps that a sample data set is obtained, basic information in the sample data set is screened based on minimum absolute contraction and a selection operator method, and risk indexes are obtained; and establishing a blastocyst transplantation feasibility prediction model, evaluating and calibrating the blastocyst transplantation feasibility prediction model by using the verification set, and finally predicting the blastocyst formation condition by using the evaluated and calibrated blastocyst transplantation feasibility prediction model. The method has reliability and repeatability, the feasibility of blastocyst transplantation can be accurately predicted, and then the formation condition of the blastocyst is accurately predicted.

The present invention discloses a blastocyst expansion and puncture device, which includes: a base, a vertical shaft installed on the base, an expansion sac covered on the vertical shaft and capable of expansion, a plurality of mold flaps, a mold-closing mechanism for driving the mold flaps to open or close, puncture holes provided on the mold flaps, and a plurality of puncture components, each puncture component including a puncture mounting plate, a plurality of puncture needles installed on the puncture mounting plate and penetrated through the puncture holes, and a puncture drive part for driving the puncture mounting plate to move in a direction toward and out of the mold; the expansion molding is completed by limiting the shape of the blastocyst after expansion through the closed mold flaps, and when the expansion molding is completed, the puncture mechanism performs puncture to form an exhaust hole on the surface of the blastocyst, and two processes can be completed by one tooling, thereby greatly improving the processing efficiency of the blastocyst.

The invention relates to a culture medium and a method for obtaining extraembryonic endoderm stem cells (XEN) from porcine blastocysts and embryonic stem cells (ESCs), and belongs to the technical field of biology. The invention develops a two-step induction method for directly converting pig ESCs into pig XEN without gene modification and two culture mediums 4FY and 2FYSC. The invention provides a high-quality model for exploring separation characteristics and intercellular communication of embryos and extraembryonic pedigree in early embryonic development of large animals, provides a good cell model for researching dynamic development of extraembryonic pedigree, and provides a high-quality material for regenerative medicine and agricultural breeding.

The invention relates to the technical field of construction of conditional gene knockout models, and discloses a construction method of a mouse Oct4 conditional gene knockout model based on quadruple sgRNA collaborative targeting, which comprises the following steps: step 1, designing four sgRNAs, namely sgRNA1, sgRNA2, sgRNA3 and sgRNA4, in which the sgRNA1 and the sgRNA2 target 5'end and 3 'end non-coding regions of a second exon of an Oct4 gene, and the sgRNA3 and the sgRNA4 target structural domain conserved sequences; step 2, preparing an electrotransfection solution, wherein the electrotransfection solution is composed of Cas9mRNA, the four sgRNAs and an electrotransfection buffer solution; step 3, carrying out electroporation treatment on the fertilized eggs of the mice; step 4, culturing the fertilized eggs subjected to the electrotransformation treatment to a blastocyst stage; and 5, carrying out genotype identification on the cultured blastocyst. According to the scheme, the sgRNA combination and embryo electrotransfection system is optimized, the mouse Oct4 conditional gene knockout model is efficiently and accurately constructed, a reliable tool is provided for regulation mechanism research of Oct4 in mouse early embryo trigerm differentiation, and the medical transformation value of Oct4 is excavated.

The invention discloses a line establishment method and a culture system of bovine trophoblast stem cells. The stem cell line is obtained by separation and induction from bovine blastocyst. The embryonic stem cell line has stability. The method can be applied to various life science and medical fields such as bovine animal breeding and breeding, gene editing models, animal cloned donor cells, medical musculoskeletal injury experiments, drug development carriers, vaccine production cell banks, embryo quality analysis and the like, and can be applied to large-scale production and application.

This invention discloses a method and system for embryo quality detection, relating to the field of embryo detection technology, including the following steps: S1: fertilized embryos are continuously cultured in an in vitro culture system containing specific metabolic marker detection components, and continuous dynamic imaging of the embryos is performed to obtain a complete dynamic developmental image sequence; S2: the dynamic developmental image sequence is segmented, and temporal developmental features are captured, identifying key time points such as pronucleus appearance and disappearance, cleavage, and blastocyst formation, and generating a segmentation mask corresponding to each frame of the dynamic developmental image sequence; this invention achieves full-process time-difference imaging to capture the temporal features of embryonic development, without trauma or interference, avoiding subjective bias in static observation; by segmenting the dynamic developmental image sequence and capturing temporal developmental features, the segmentation accuracy is high and the anti-interference ability is strong.

The present invention belongs to the field of in vitro embryo technology and relates to the technology of in vitro cultivation of pre-implantation embryos of mammals. The present invention provides the use of dihydromyricetin or its derivatives in the preparation of products for improving the quality and developmental ability of in vitro embryos, and products for improving the developmental ability of in vitro embryos, the use of dihydromyricetin or its derivatives in improving the quality of in vitro embryos, and a method for improving the developmental ability of in vitro embryos. The present invention discovers that dihydromyricetin or its derivatives can increase the blastocyst rate of in vitro embryo culture, reduce the content of cellular reactive oxygen species, increase the content of cellular glutathione, and promote the expression of oxidative stress genes, thereby effectively improving the quality and developmental ability of in vitro embryos.

The invention provides a construction method of a Kdm2a gene systemic knockout mouse model and a systemic knockout mouse model.The construction method comprises the steps that targeting vector plasmids are constructed and then transferred into mouse embryonic stem cells, and the obtained positive embryonic stem cells are injected into a wild type mouse blastocyst stage embryo to cultivate a chimera mouse; carrying out selfing on the chimera mouse to obtain a Kdm < 2 > afl / -mouse, and carrying out selfing on the obtained Kdm < 2 > afl / fl mouse again to obtain a Kdm < 2 > afl / fl mouse; hybridizing the male Kdm2afl / fl mouse with a female Zp3-Cre mouse, so as to obtain a Kdm2a cKO mouse of which the oocyte is specifically knocked out; the method comprises the following steps: hybridizing a female Kdm2a cKO mouse with a wild type mouse, and screening to obtain a maternal Kdm2a deleted mouse; the maternal Kdm2a deleted mouse is subjected to selfing, and the Kdm2a gene systemic knockout mouse Kdm2a gKO is obtained. According to the method, the problem that the mouse embryo is lethal after Kdm2a systemic knockout is solved.

The present application discloses an artificial propagation-hybridization combined breeding method for meat goats, which relates to the technical field of animal breeding. The specific steps include: selecting a goat breed with good meat performance as the maternal parent and selecting a goat breed with a large body as the paternal parent; culturing the maternal oocytes in vitro to mature oocytes containing the first polar body, using a temperature gradient screening device to screen out highly motile sperm, fertilizing the mature oocytes with the highly motile sperm in vitro, culturing them to a hybrid blastocyst morphology, transplanting them into the maternal body to develop until natural delivery, and obtaining a hybrid F1 generation; backcrossing the F1 generation with the maternal parent, and backcrossing the backcross offspring with the maternal parent multiple times until the breeding goal is met, and then cross-crossing and fixing them to obtain a new breed / line with excellent traits; improving the sperm screening efficiency by utilizing the screening device, ensuring the quality of the screened sperm, and thus significantly improving the fertilization efficiency and embryo survival rate.

The application relates to a mouse model construction method for psoriasis research based on SBSN 600 lysine dihydroxyisobutyryl modification, which comprises the following steps: first step, constructing a plasmid comprising a DNA fragment of a SBSN gene sequence, wherein 600 sites of the SBSN sequence are mutated from lysine (K) to threonine (T); second step, culturing mouse embryonic stem cells; third step, electrically transforming the embryonic stem cells; fourth step, drug screening; fifth step, culturing the embryonic stem cells screened in the fourth step; sixth step, injecting the embryonic stem cells cultured in the fourth step into mouse blastocysts, then culturing the mouse blastocysts, and transplanting the cultured blastocysts into the uterus of a pseudopregnant female mouse, wherein the mouse delivered by the female mouse is a chimera mouse; and seventh step, breeding the chimera mouse and identifying the mouse genotype through PCR.

The present disclosure relates to a method for constructing a mouse model with short telomere. Specifically, the present disclosure obtains fertilized eggs by fertilizing the sperms and eggs of mice in vitro, and the fertilized eggs are cultured to the blastocyst stage in vitro and then transferred into the surrogate female mouse for development, thereby producing mice with short telomere. The method of the present disclosure does not require gene editing, has a short modeling cycle, and the effect is reliable and stable. By changing only the environment during embryo transfer, the telomere elongation process in the embryo is interfered, so as to successfully construct a progeny model with short telomere. There is no significant effect on the reproductive rate of female mice. Therefore, the present disclosure can provide a method for constructing a mouse model with short telomere for exploring the mechanism of telomere shortening and studying telomere-related phenotypes such as aging.

This invention relates to the fields of biomedical engineering and embryo quality assessment, and particularly to a biomarker for assessing the quality of IVM-derived embryos and its application. The biomarker includes time point parameters t2 and t9 of the IVM-derived embryos, and time interval parameters S2, t9–t7, t9–t8, or tSB–t9. A method for assessing the quality of IVM-derived embryos based on the aforementioned biomarker has been established. This method combines non-invasive temporal imaging technology with morphodynamic multi-parameter analysis, providing a reliable tool for assessing the blastocyst formation potential and chromosomal euploidy of IVM-derived embryos in clinical practice, thus potentially expanding the application of IVM technology in assisted reproductive technology.

This invention discloses a method, apparatus, electronic device, and storage medium for blastocyst quality assessment. The method includes: acquiring a sequence of images of a target blastocyst to be tested, the sequence comprising multiple images of the target blastocyst at different time points; processing the sequence of images of the target blastocyst to be tested based on a blastocyst quality assessment model to obtain a quality assessment result; the blastocyst quality assessment model includes a global feature extraction module, a temporal feature extraction module, and a result determination module; the global feature extraction module sequentially extracts global features from the images to be tested in the sequence, obtaining global features corresponding to each image; the temporal feature extraction module extracts temporal features from the global features corresponding to each image, obtaining temporal features; and the result determination module classifies the temporal features to obtain a quality assessment result, achieving accurate assessment of blastocyst quality and providing accurate data support for subsequent decision-making.

The invention discloses a blastocyst flaring puncture device which comprises a base, a vertical shaft installed on the base, an expansion bag wrapping the vertical shaft and capable of being expanded, a plurality of mold petals, a mold closing mechanism used for driving the mold petals of a mold to be opened or closed, puncture holes formed in the mold petals and a plurality of puncture assemblies. Each puncture assembly comprises a puncture mounting plate, a plurality of puncture needles which are mounted on the puncture mounting plate and penetrate through the puncture holes, and a puncture driving part which is used for driving the puncture mounting plate to move towards the interior of the mold and in the direction of retreating from the interior of the mold; the shape of the blastocyst after bulging is limited through the closed die petals to complete flaring forming, when flaring forming is completed, the puncture mechanism conducts puncture, exhaust holes are formed in the surface layer of the blastocyst, two procedures can be completed through one-time tooling, and the machining efficiency of the blastocyst is greatly improved.

The invention belongs to the technical field of biology, and particularly relates to application of astragaloside in regulation of parthenogenetic activation embryo development. According to the method disclosed by the invention, the maturation rate of the oocytes can be obviously improved by adding substances such as astragaloside into the sheep oocyte in-vitro maturation liquid. The preparation method comprises the following steps: firstly, respectively adding lycopene, astragaloside, isoliquiritigenin, forsythin and baicalein into an oocyte maturation solution, and screening out the optimal dosage of each additive according to the first polar body discharge rate, the cleavage rate and the blastocyst rate; and finally, adding an additive combination into the oocyte maturation liquid according to the screened optimal dosage, detecting the first polar body discharge rate, the cleavage rate and the blastocyst rate to determine an optimal composition, and detecting the levels of ROS, GSH, mitochondrial membrane potential, oxidative stress and apoptosis related genes of the optimal composition to further determine the application effect of the composition.

The application provides a culture medium and method for stem cell differentiation into macrophages, and relates to the technical field of biotechnology.The culture medium provided by the application comprises six kinds of culture media, the components of which are clear, and the culture media and cytokines are prepared without animal sources, so that exogenous substances are avoided, the differentiated cells are safer, and clinical transformation can be seamlessly connected.The preparation of macrophages by using the culture medium does not need to pass through an EB process, so that the steps are simplified, the differentiation time is shortened, the purity, uniformity and stability of differentiation are improved.The method for differentiating macrophages provided by the application skips the complicated blastocyst formation stage, simplifies the differentiation steps, greatly shortens the differentiation period, reduces unknown risks, improves the differentiation efficiency and stability, can obtain a large amount of high-purity macrophages with killing capacity in a short period, and also provides the possibility of full-closed automatic production for large-scale production preparation, and solves the bottleneck of industrialization difficulty.

The present disclosure relates to a method implemented by a computer system, and an oocyte quality analysis system and a non-transitory computer-readable medium that stores instructions, and particularly relates to a system and method for assessing the viability of an oocyte. In some embodiments, an image sequence related to an oocyte drawn into a pressure tool is acquired. By using a segmentation model, objects related to the oocyte can be identified. On the basis of the identified objects, features such as morphological features and the drawing depth related to the oocyte can be determined. Then, at least some of the features can be input into a machine learning model to generate an oocyte grade that indicates the probability of the oocyte developing into a usable blastocyst. Optionally, the oocyte grade can be presented to a user by means of an interactive user interface.

The invention discloses an application of an Odf4 gene or protein as a biomarker in evaluating the ability of sperms to bind to oocytes. According to the invention, the interaction between Odf4 protein and sperm-egg binding key factors ZP3R and ADAM2 is found for the first time through experiments, and a sperm-egg recognition complex is formed; odf4 gene deletion can lead to significant reduction of the ability of sperms to bind to oocytes, and the formation rates of 2-cells, 4-cells, 8-cells and blastocysts of fertilized embryos are significantly reduced. On the basis, by detecting the expression level or functional integrity of the ODF4 in the sperms, the binding capacity of the sperms and oocytes and the development potential of fertilized embryos can be objectively evaluated. According to the invention, the limitation of low detection rate of recessive sperm-egg binding defects in the prior art is overcome, and an efficient molecular tool is provided for etiological typing, assisted reproduction scheme selection and fertility prediction of male infertility.