187 results about "Blastocyst" patented technology

The invention discloses a culture solution for reducing lipid of animal embryos before freezing as well as a preparation method and application of the culture solution. The culture solution is prepared by adding bezafibrate and cholic acid into an NCSU23 culture solution. The invention also discloses a preparation method of the culture solution, which comprises the following steps: preparing an NCSU23 culture solution, and adding bezafibrate and cholic acid into the NCSU23 culture solution. Compared with a conventional NCSU23 culture solution, the culture solution provided by the invention has the advantages that the added bezafibrate and cholic acid have a synergistic lipid-lowering effect, so that the content of lipid in an embryo can be remarkably reduced, and the survival rate and the blastocyst hatching rate after the embryo is frozen and unfrozen are finally effectively improved. The culture solution provided by the invention has application prospects in the aspects of animal embryo lipid reduction, frozen embryo survival and development and the like.

The invention discloses an image detection and evaluation method for an unfrozen form of a fertilized egg embryo. The method comprises the following steps: acquiring a time sequence image sequence after the fertilized egg embryo is unfrozen; performing image standardization processing on the time sequence image sequence to generate a standardized embryo image set; extracting multi-dimensional embryo quality parameters from the standardized embryo image set; wherein the multi-dimensional embryo quality parameters comprise a morphological static parameter set, a dynamic change parameter set and a texture feature parameter set; generating a blastocyst formation rate based on the multi-dimensional embryo quality parameters; outputting a third-level embryo quality evaluation result according to a comparison relationship between the blastocyst formation rate and a preset threshold interval; wherein the three-stage embryo quality evaluation result comprises an excellent embryo grade, a qualified embryo grade and an abnormal embryo grade. The method has the following advantages and effects: full-automatic and multi-dimensional quantitative evaluation of the thawing process of the unfrozen embryos is realized, so that the scientificity of embryo screening and the success rate of clinical pregnancy are improved.

The present invention provides: a drug which can improve the implantation rate of an embryo and can be used in conjunction with conventional drugs for infertility treatment; and a screening method and the like with which it is possible to reliably evaluate such a drug. Specifically, the integrin activator comprises: (1) a peptide composed of KFEERMRCKWMT, and (2) a peptide composed of KFEERMRCKWMT. (2) a peptide which is composed of KFEEERSRCKWMT; (3) a peptide comprising an amino acid sequence obtained by deleting, substituting and / or adding 1-3 amino acids in (1) or (2); or (4) a peptide obtained by binding any one of (1) to (3) to a membrane-permeable molecule. Provided are an artificial uterus and a screening method based on an implantation reproduction test system using a fertilized egg (for example, an embryo developed to an implanted blastocyst), the artificial uterus comprising: a hydrogel containing endometrial stromal cells and having an extracellular matrix as a main component; and a uterine epithelial organoid configured to be surrounded by the hydrogel.

The invention provides biopsy liquid and application thereof in pig blastocyst laser cutting, and belongs to the technical field of animal biology. The invention provides a biopsy solution and a preparation method thereof, the biopsy solution can be applied to pig blastocyst biopsy by laser cutting, the problem that at present, no adaptive biopsy solution exists for laser cutting of pig blastocyst is solved, and the invention further provides a method for laser cutting of pig blastocyst by using the biopsy solution. The problems of embryo cell injury, embryo development potential damage and the like possibly caused by laser cutting of the pig blastocyst in the prior art are solved. The biopsy liquid and the use method thereof can be efficiently and accurately applied to acquisition of pig blastocyst trophoblast cells, and are low in cost and good in repeatability.

The invention belongs to the field of antibody development, and discloses a screening method and application of a human-mouse protein high homologous target antibody based on a fully humanized antibody mouse. Aiming at the problem of weak antibody response caused by immune tolerance of human-mouse high homologous targets (protein homology is greater than or equal to 95%), the following scheme is provided: in embryonic stem cells (ES cells) of HUGO-Mabfully humanized antibody transgenic mice, a mouse target gene (such as ACVR2A) is knocked out through a Turbo Knockout technology, and homozygous knockout ES clones are screened; carrying out microinjection on the clones to the whitened B6 mouse blastocyst, and transplanting a pregnant mouse to obtain a Founder mouse; the Founder mouse is subjected to target antigen immunization for more than or equal to 4 times (the Freund's complete adjuvant is used for the first time), and the titer of the serum antibody is detected. According to the invention, 100% homozygous knockout chimeric efficiency is realized in the Founder stage, the mouse construction period is shortened from traditional 8-10 months to 3-4 months, and the diversity and affinity of the antibody are significantly improved (titer reaches 1: 729,000). The obtained antibody can be used for preparing medicines for treating tumors or autoimmune diseases.

The invention provides an improved blastocyst culture solution as well as a preparation method and application thereof, and relates to the field of embryonic cell in-vitro culture. Based on a formula of a conventional blastocyst culture solution, colony stimulating factor-1 (CSF-1) and a transforming growth factor-alpha (TGF-alpha) are added, and the colony stimulating factor-1 and the transforming growth factor-alpha are dissolved in the conventional culture solution according to the concentration of 0.02-0.06 mmol / L and the concentration of 0.01-0.05 mmol / L; the obtained improved blastocyst culture solution can improve the mulberry embryo formation rate or blastocyst formation rate in in-vitro culture, promote the development of low-quality embryos and improve the mulberry embryo quality or blastocyst quality, and can be used for preparing medicines for promoting embryo implantation or subsequent growth and development.

The invention belongs to the field of biomedical image processing, and relates to a blastocyst-stage embryo morphological quality evaluation method based on multi-modal information, which comprises the following steps: acquiring an embryo microscopic image and clinical data of a patient, and acquiring a trained embryo morphological quality evaluation model, based on the trained embryo morphological quality evaluation model, performing knowledge distillation according to the embryo microscopic image and clinical data of the patient to obtain a student model; obtaining an embryo microscopic image and clinical data of a patient, and inputting the embryo microscopic image and the clinical data of the patient into the student model to obtain an evaluation result; the embryo morphological quality evaluation model comprises a multi-modal feature extraction module, a comparison module, a multi-modal feature fusion module and a multi-task embryo quality rating module; according to the method, the comparison learning mechanism is utilized to align the embryology evaluation text information with the image features, so that the evaluation accuracy is improved.

The present invention relates to a blastocyst quality evaluation method and system. The method comprises: inputting a blastocyst multi-focus image into a pre-trained deep learning model to obtain a blastocyst development stage and quantitative scores for ICM and TE; obtaining a blastocyst quality quantitative score by means of the quantitative scores for ICM and TE; and selecting an optimal blastocyst by means of the blastocyst development stage and the blastocyst quality quantitative score. According to the present invention, the quality of blastocysts of the same grade is distinguished, thereby further quantifying the overall quality of an embryo.

The invention discloses a blastocyst image instance segmentation method and device, computer equipment and a storage medium. The method comprises the following steps: acquiring a blastocyst image to be segmented; preprocessing the to-be-segmented blastocyst image to obtain a preprocessed to-be-segmented blastocyst image; inputting the preprocessed blastocyst image to be segmented into an instance segmentation model for instance segmentation of internal components to obtain a segmentation result; wherein the instance segmentation model is a model obtained by amplifying a blastocyst image through data enhancement, preprocessing the blastocyst image to form a sample set, and training a deep learning network by using the sample set; and outputting the segmentation result. By implementing the method provided by the invention, the segmentation of the cell mass and the trophoderm in the human blastocyst image can be realized, the morphological characteristics of the cell mass and the trophoderm can be extracted, and the problems existing in blastocyst image analysis can be better solved.

The invention relates to a mouse model construction method for psoriasis research based on dihydroxyisobutyrylation modification of SBSN 600 site lysine. The mouse model construction method comprises the following steps: 1, constructing a plasmid comprising an SBSN gene sequence and a DNA fragment of which the 600 site of the SBSN sequence is mutated from lysine (K) to threonine (T); 2, culturing the mouse embryonic stem cells; step 3, carrying out electric transformation on the embryonic stem cells; 4, screening the medicines; 5, calling and culturing the embryonic stem cells screened in the step 4; step 6, injecting the embryonic stem cells called and cultured in the step 4 into a mouse blastocyst, then culturing the mouse blastocyst, and transplanting the cultured blastocyst into the uterus of a pseudopregnant female mouse, so that the mouse generated by delivery of the female mouse is a chimeric mouse; and 7, breeding the chimeric mouse and identifying the genotype of the mouse through PCR (Polymerase Chain Reaction).

The utility model relates to a thermoplastic polymer pipe variable-diameter thinning mould and equipment capable of ensuring the shape of a conical end part, which comprises a first module and a second module, a first mould cavity of the first module comprises a first equal-diameter pipe cavity, a first conical pipe cavity and a first connecting cavity between the first equal-diameter pipe cavity and the first conical pipe cavity; a second die cavity of the second die set comprises a second equal-diameter pipe cavity, a second taper pipe cavity and a second connecting cavity between the second equal-diameter pipe cavity and the second taper pipe cavity, the inner diameter of the second equal-diameter pipe cavity is larger than the inner diameter of the first equal-diameter pipe cavity and smaller than the outer diameter of the pipe, the die cavity comprises an equal-diameter cavity and a taper pipe cavity, and the taper of the taper pipe cavity is consistent with the taper of the taper end of a finished balloon product. And the shape and the taper of the end part of the blastocyst can be kept while the pipes at the two ends of the blastocyst are thinned. Meanwhile, the conical end part of the balloon embryo can be heated and stretched when being positioned in the conical tube cavity, and after the step, the end part even does not need to be stretched and shaped independently, but only needs to be shaped together with the whole balloon, so that the processing technology is simplified, and the production efficiency is improved.

The invention discloses an application of creatine in improving the development rate of an in-vitro blastocyst of a sheep oocyte. The concentration of the creatine is 0.5 mmol / L. The creatine is added into an in-vitro maturation system and an in-vitro embryo culture system. The key metabolite creatine is analyzed and screened based on metabonomics of sheep oocytes, early embryos, follicular fluid and fallopian tube fluid. On the basis, the development regulation effect of the creatine on the oocytes and early embryos is researched, the in-vitro blastocyst development rate of the sheep oocytes can be remarkably increased by adding the creatine into an in-vitro maturation system and an in-vitro embryo culture system of the sheep oocytes, and it is proved that the creatine can serve as an additive of the in-vitro culture system; and the creatine is a key metabolite contained in the development microenvironment and is friendly to oocyte and embryo development.

The invention belongs to the technical field of assisted reproduction, and particularly relates to a glucose slow-release structure used in an embryo in-vitro culture solution, a preparation process and a use method. The glucose slow-release structure comprises at least one inner layer containing glucose and a biodegradable material outer layer wrapping the inner layer, and the biodegradable material outer layer comprises a matrix with a plurality of micropores and at least one sealing material layer used for sealing pores of the micropores. According to the invention, gradient release from low sugar in the cleavage stage to high sugar in the blastocyst stage is realized, midway liquid change or artificial sugar supplement is not needed, the operation is obviously simplified, and pollution and mechanical stress risks are reduced.

Described are small molecule cocktails that enable de novo derivation and long-term culture of bovine extraembryonic endoderm cells (bXENs). Methods of using the small molecule cocktails to generate bXENs are also described. Methods of using the bXENs to form blastocyst models or to maintain the stemness of bovine ESCs and prevent them from differentiation are also described.

The invention relates to the technical field of livestock and poultry breeding, in particular to a goat hybridization breeding method capable of achieving complete-cycle nutrition enhancement. The core of the method is that systematic nutrition intervention runs through the whole breeding process. Before hybridization, performing differential nutrition pretreatment on the parents for 60 days: feeding the female parents with a breeding nutrition enhancer added with components such as D-chiro-inositol and N-acetylcysteine, and supplementing sperm motility enhancer containing acetylated L-carnitine and ergothioneine to the male parents. In the gamete treatment stage, the oocytes are subjected to in-vitro maturation culture in a special culture solution containing components such as D-chiro-inositol and a growth differentiation factor 9; after the sperms are subjected to gradient centrifugal screening, completing in-vitro fertilization in an optimized fertilization culture solution; after the embryos develop into blastocysts in the sequential culture system, carrying out laparoscopic transplantation, and carrying out staged precise nutrition culture on the pregnant ewes. According to the method, through multi-level nutrition enhancement, the reproductive capacity of the goats and the growth performance of offspring are effectively improved.

It is revealed that an organ such as pancreas can be regenerated by utilizing a fact that the deficiency of an organ is complemented by injecting an induced pluripotent stem cell (iPS cell) into a developed blastocyst in a blastocyst complementation method. Thus, the present invention has solved the above-described object. This provides a method for producing a target organ, using an iPS cell, in a living body of a non-human mammal having an abnormality associated with a lack of development of the target organ in a development stage, the target organ produced being derived from a different individual mammal that is an individual different from the non-human mammal.

Provided are a reagent combination or a kit for constructing an embryoid and the use thereof. The reagent combination or the kit can be used for inducing a stem cell to produce a blastocyst-like lineage precursor cell, a blastocyst-like cell and / or an embryoid, which are based on small-molecule induction and independent of transgenes, and have a single cell source. The produced blastocyst-like lineage precursor cell and blastocyst-like cell have a full blastocyst lineage, and are seed cells having balanced developmental competence and totipotency, which solves the problem of unbalanced cell maturation in the prior art. The produced embryoid is highly similar to natural embryos in terms of morphological characteristics and transcriptomic characteristics, has the capability of highly reproducing post-implantation embryonic development and / or gastrulation, which is beneficial for research on embryonic development in vitro. The method for inducing the stem cell to produce the blastocyst-like lineage precursor cell, the blastocyst-like cell and / or the embryoid by using the reagent combination or the kit is simple, and has high applicability and high embryoid construction efficiency.

The present invention relates to a medium composition for in vitro fertilization and / or in vitro culture comprising a compound represented by chemical formula 1 or a pharmaceutically acceptable salt thereof, and a method using same. The compound or composition suppresses reactive oxygen species and lipid peroxidation in ovarian granulosa cells to preventing cytotoxicity and mitochondrial dysfunction caused by apoptosis and ferroptosis, leading to an improvement in the quality of aged oocytes, and to increase the developmental rate and blastocyst formation rate of pre-implantation embryos, thereby improving the efficiency of in vitro fertilization. In addition, treatment with the medium composition during the vitrification process of in vitro–fertilized embryos improves re-expansion and survival rates, thereby reducing structural and metabolic damage. Accordingly, the present invention can be advantageously applied to an assisted reproductive technology for treating infertility and subfertility, and in vitro fertilization and / or in vitro culture for improving the propagation efficiency of livestock.

The invention relates to an artificial intelligence prediction method and system for a multi-embryo blastocyst culture outcome. The method comprises the following steps: acquiring morphological parameter data of embryo culture; constructing a feature vector based on the morphological parameter data; and inputting the feature vector into a preset classifier, and predicting a culture result of the embryo group. The method is suitable for various culture environments, and not only can treat group culture, but also can be applied to period prediction of individual culture. The diversity meets the gap among different culture strategies in the prior art; while the dependence on individual labels is reduced, the prediction accuracy of the method is equivalent to that of an existing method based on single embryo probability prediction, and the technical reliability is ensured.

The invention discloses the use of tributyrin as an additive for embryo in vitro development culture medium, and belongs to the technical field of animal reproductive biology. The invention applies tributyrin as an additive to mouse embryo in vitro development culture medium for the first time, and the tributyrin can significantly improve the blastocyst rate, reduce the ROS content in the embryo, improve the mitochondrial membrane potential in the embryo, improve the ATP level in the embryo and the expression of antioxidant genes, improve the DNA methylation and histone modification level in the embryo, and promote the embryo in vitro development; and tributyrin, as a natural antioxidant and epidrug, is safe and has no toxic side effects, is cheap and easy to obtain, does not need to replace the culture medium during the entire development and culture process, and is simple to operate; the tributyrin provides strong support for in vitro embryo engineering technologies such as the efficient development of embryos in human assisted reproductive technology, mammalian fertilized embryos, parthenogenetic embryos and somatic cell cloned embryos, and has good application prospects.

The invention provides an oocyte in-vitro maturation culture solution additive and application thereof. The additive is tert-butylhydroquinone. By adding 100 [mu] g / mL of TBHQ into a basic culture solution, the first polar body discharge rate of oocytes is remarkably increased to 77.29%, the ROS level is reduced, and the blastocyst rate of in-vitro fertilized embryos is increased. The technology is suitable for breed conservation of livestock genetic resources such as sheep and can replace live livestock transportation to reduce the cost.

The invention discloses a blastocyst transplantation feasibility prediction model construction method, system and device, a medium and a product, and relates to the field of result prediction.The method comprises the steps that a sample data set is obtained, basic information in the sample data set is screened based on minimum absolute contraction and a selection operator method, and risk indexes are obtained; and establishing a blastocyst transplantation feasibility prediction model, evaluating and calibrating the blastocyst transplantation feasibility prediction model by using the verification set, and finally predicting the blastocyst formation condition by using the evaluated and calibrated blastocyst transplantation feasibility prediction model. The method has reliability and repeatability, the feasibility of blastocyst transplantation can be accurately predicted, and then the formation condition of the blastocyst is accurately predicted.

The present invention discloses a blastocyst expansion and puncture device, which includes: a base, a vertical shaft installed on the base, an expansion sac covered on the vertical shaft and capable of expansion, a plurality of mold flaps, a mold-closing mechanism for driving the mold flaps to open or close, puncture holes provided on the mold flaps, and a plurality of puncture components, each puncture component including a puncture mounting plate, a plurality of puncture needles installed on the puncture mounting plate and penetrated through the puncture holes, and a puncture drive part for driving the puncture mounting plate to move in a direction toward and out of the mold; the expansion molding is completed by limiting the shape of the blastocyst after expansion through the closed mold flaps, and when the expansion molding is completed, the puncture mechanism performs puncture to form an exhaust hole on the surface of the blastocyst, and two processes can be completed by one tooling, thereby greatly improving the processing efficiency of the blastocyst.

The invention relates to the technical field of biology, and provides a composition, a culture solution, a product and a method for promoting embryo ectogenesis. The composition is prepared from 8-Br-cGMP and glycyrrhizic coumarin. The composition can reduce the lipid peroxidation level of embryos and reduce the toxic effect of lipid peroxidation on the embryos. Meanwhile, fat hydrolysis and oxidation energy supply can be promoted, and sufficient energy guarantee is provided for in-vitro development of early embryos. In addition, the composition can effectively reduce the active oxygen level in the embryo and reduce the damage of oxidative stress to the embryo. By adopting the composition disclosed by the invention, the embryo ectogenesis can be promoted, and the in-vitro blastocyst formation rate can be increased.

The invention relates to a culture medium and a method for obtaining extraembryonic endoderm stem cells (XEN) from porcine blastocysts and embryonic stem cells (ESCs), and belongs to the technical field of biology. The invention develops a two-step induction method for directly converting pig ESCs into pig XEN without gene modification and two culture mediums 4FY and 2FYSC. The invention provides a high-quality model for exploring separation characteristics and intercellular communication of embryos and extraembryonic pedigree in early embryonic development of large animals, provides a good cell model for researching dynamic development of extraembryonic pedigree, and provides a high-quality material for regenerative medicine and agricultural breeding.

The invention relates to the technical field of construction of conditional gene knockout models, and discloses a construction method of a mouse Oct4 conditional gene knockout model based on quadruple sgRNA collaborative targeting, which comprises the following steps: step 1, designing four sgRNAs, namely sgRNA1, sgRNA2, sgRNA3 and sgRNA4, in which the sgRNA1 and the sgRNA2 target 5'end and 3 'end non-coding regions of a second exon of an Oct4 gene, and the sgRNA3 and the sgRNA4 target structural domain conserved sequences; step 2, preparing an electrotransfection solution, wherein the electrotransfection solution is composed of Cas9mRNA, the four sgRNAs and an electrotransfection buffer solution; step 3, carrying out electroporation treatment on the fertilized eggs of the mice; step 4, culturing the fertilized eggs subjected to the electrotransformation treatment to a blastocyst stage; and 5, carrying out genotype identification on the cultured blastocyst. According to the scheme, the sgRNA combination and embryo electrotransfection system is optimized, the mouse Oct4 conditional gene knockout model is efficiently and accurately constructed, a reliable tool is provided for regulation mechanism research of Oct4 in mouse early embryo trigerm differentiation, and the medical transformation value of Oct4 is excavated.

The invention discloses a line establishment method and a culture system of bovine trophoblast stem cells. The stem cell line is obtained by separation and induction from bovine blastocyst. The embryonic stem cell line has stability. The method can be applied to various life science and medical fields such as bovine animal breeding and breeding, gene editing models, animal cloned donor cells, medical musculoskeletal injury experiments, drug development carriers, vaccine production cell banks, embryo quality analysis and the like, and can be applied to large-scale production and application.

This invention discloses a method and system for embryo quality detection, relating to the field of embryo detection technology, including the following steps: S1: fertilized embryos are continuously cultured in an in vitro culture system containing specific metabolic marker detection components, and continuous dynamic imaging of the embryos is performed to obtain a complete dynamic developmental image sequence; S2: the dynamic developmental image sequence is segmented, and temporal developmental features are captured, identifying key time points such as pronucleus appearance and disappearance, cleavage, and blastocyst formation, and generating a segmentation mask corresponding to each frame of the dynamic developmental image sequence; this invention achieves full-process time-difference imaging to capture the temporal features of embryonic development, without trauma or interference, avoiding subjective bias in static observation; by segmenting the dynamic developmental image sequence and capturing temporal developmental features, the segmentation accuracy is high and the anti-interference ability is strong.

The invention provides a trophoblast cell analysis method, device and equipment and a storage medium, and relates to the technical field of embryo quality evaluation. Comprising the steps of collecting a multi-focal plane image of a target blastocyst; performing image fusion on the multi-focal plane image based on a pre-trained image fusion model to generate a first fusion image and a second fusion image corresponding to the target blastocyst; performing main body region segmentation on the first fusion image and the second fusion image to obtain a first target image and a second target image; segmenting the trophoblast cells in the first target image and the second target image by adopting a pre-trained image segmentation model to obtain a first mask image and a second mask image; according to the first mask image and the second mask image, multiple feature parameters of the trophoblast cells are extracted, and based on the feature parameters, correlation between the feature parameters and the development result of the embryo is obtained through analysis. According to the method, a comprehensive interpretable feature analysis method is realized, and the analysis accuracy is improved.

The present invention belongs to the field of in vitro embryo technology and relates to the technology of in vitro cultivation of pre-implantation embryos of mammals. The present invention provides the use of dihydromyricetin or its derivatives in the preparation of products for improving the quality and developmental ability of in vitro embryos, and products for improving the developmental ability of in vitro embryos, the use of dihydromyricetin or its derivatives in improving the quality of in vitro embryos, and a method for improving the developmental ability of in vitro embryos. The present invention discovers that dihydromyricetin or its derivatives can increase the blastocyst rate of in vitro embryo culture, reduce the content of cellular reactive oxygen species, increase the content of cellular glutathione, and promote the expression of oxidative stress genes, thereby effectively improving the quality and developmental ability of in vitro embryos.