Automated system for cryopreservation of oocytes, embryos, or blastocysts

a technology of cryopreservation and embryo, applied in the field of cryopreservation, can solve the problems of oocytes often suffering structural damage, conventional techniques are typically labor-intensive, and it is difficult to reproduce in an effective, efficient and consistent manner

Inactive Publication Date: 2011-08-25
MARIPOSA BIOTECH
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AI Technical Summary

Benefits of technology

[0005]Various embodiments of the present invention provide a method of cryopreservation and reanimation of oocytes, embryos or blastocysts. The method comprises positioning one or more oocytes, embryos or blastocysts in a processing container, the processing container being configured to allow fluid to flow into and out of the processing container; and flowing two or more fluids into ...

Problems solved by technology

However, conventional techniques have proven difficult to reproduce in an effective, efficient and consistent manner.
Such conventional techniques are typically labor-intensive, requiring substantial handling of the oocytes, embryos or blastocysts by a highly skilled human technician.
Further, oocytes frequently incur structural damage during conventional cryopreservation techniques.
For example, conventional manual movement of oocytes among cryopreservation solution baths can impart osmotic and thermal shock.
For instance, formation of ice crystals within the oocyte can cause in...

Method used

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  • Automated system for cryopreservation of oocytes, embryos, or blastocysts
  • Automated system for cryopreservation of oocytes, embryos, or blastocysts
  • Automated system for cryopreservation of oocytes, embryos, or blastocysts

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Embodiment Construction

[0026]As used herein, the following definitions shall apply unless otherwise indicated.

[0027]The term “oocyte” refers to an unfertilized freshly harvested or mature oocyte and refers to both the singular and plural regardless of whether this term employs terms such as “a”, “the” and the like. The freshly harvested means that the oocytes were harvested from the animal donor within 8 hours of initiation of the stabilization / cryopreservation process, preferably within about 4 hours of initiation of the stabilization / cryopreservation process, more preferably within about 1 hour of initiation of the stabilization / cryopreservation process, and even more preferably within about 0.1 hour of initiation of the stabilization / cryopreservation process. The mature oocytes mean harvested oocytes which are graded on a maturation scale as “mature stage—MII.” This scale further identifies harvested oocytes as “intermediate stage—(MI)” or “immature stage—(GV)”.

[0028]The term “reanimated oocytes” refer...

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Abstract

An automated system and method of cryopreservation and reanimation of oocytes, embryos, or blastocysts is disclosed. One or more oocytes or embryos are positioned in a processing container, the processing container being configured to allow fluid to flow into and out of the processing container, where two or more fluids flow into and out of the processing container with oocytes or embryos therein. The temperature of the fluid may be controlled in the processing container according to predetermined requirements. The flowing of the fluids may be controlled by a central controller adapted to control one or more valves.

Description

FIELD OF THE INVENTION[0001]The present invention relates generally to the field of cryopreservation. In particular, the present invention relates to systems and methods for providing automated change in the solution environment for the cryopreservation and / or reanimation of oocytes, embryos or blastocysts.BACKGROUND OF THE INVENTION[0002]This section is intended to provide a background or context to the invention that is recited in the claims. The description herein may include concepts that could be pursued, but are not necessarily ones that have been previously conceived or pursued. Therefore, unless otherwise indicated herein, what is described in this section is not prior art to the description and claims in this application and is not admitted to be prior art by inclusion in this section.[0003]The ability to cryopreserve and then reanimate oocytes, embryos or blastocysts is desirable for many reasons. However, conventional techniques have proven difficult to reproduce in an ef...

Claims

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Application Information

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IPC IPC(8): C12Q3/00C12M3/00C12N5/075C12Q1/02C12N5/073C12M1/34G05D7/00G06F19/00
CPCA01N1/0252A01N1/0221
Inventor BURBANK, FREDJONES, MICHAEL
Owner MARIPOSA BIOTECH
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