17 results about "Blastula" patented technology

Provided are a reagent combination or a kit for constructing an embryoid and the use thereof. The reagent combination or the kit can be used for inducing a stem cell to produce a blastocyst-like lineage precursor cell, a blastocyst-like cell and / or an embryoid, which are based on small-molecule induction and independent of transgenes, and have a single cell source. The produced blastocyst-like lineage precursor cell and blastocyst-like cell have a full blastocyst lineage, and are seed cells having balanced developmental competence and totipotency, which solves the problem of unbalanced cell maturation in the prior art. The produced embryoid is highly similar to natural embryos in terms of morphological characteristics and transcriptomic characteristics, has the capability of highly reproducing post-implantation embryonic development and / or gastrulation, which is beneficial for research on embryonic development in vitro. The method for inducing the stem cell to produce the blastocyst-like lineage precursor cell, the blastocyst-like cell and / or the embryoid by using the reagent combination or the kit is simple, and has high applicability and high embryoid construction efficiency.

The invention discloses a dairy cow early embryo sex identification method, and belongs to the technical field of dairy cow breeding and breeding. The identification method comprises the following steps: (1) culturing a dairy cow embryo to an early blastocyst stage, starting to perform single-embryo single-droplet culture, and after culturing until a blastocyst cavity is formed, punching a blastocyst zona pellucida to promote the blastocyst to shrink and release blastocyst cavity liquid; (2) collecting blastocyst cavity liquid, and extracting embryo free DNA in the blastocyst cavity liquid; (3) by taking the free DNA as a template, designing a primer to amplify a specific repetitive sequence ChrY fragment of the bull Y chromosome; if the amplification product exists, the embryo is identified as a bull, and if the amplification product does not exist, the embryo is identified as a cow. The method can ensure that the embryo free DNA in the obtained blastocyst cavity fluid is only derived from the embryo, and can effectively avoid embryo injury. According to the method disclosed by the invention, the copy number of the amplified target area in the bull Y chromosome is more than 2000 times, a detection signal can be amplified, and the detection accuracy is improved.

The invention belongs to the technical field of biology, and particularly relates to application of astragaloside in regulation of parthenogenetic activation embryo development. According to the method disclosed by the invention, the maturation rate of the oocytes can be obviously improved by adding substances such as astragaloside into the sheep oocyte in-vitro maturation liquid. The preparation method comprises the following steps: firstly, respectively adding lycopene, astragaloside, isoliquiritigenin, forsythin and baicalein into an oocyte maturation solution, and screening out the optimal dosage of each additive according to the first polar body discharge rate, the cleavage rate and the blastocyst rate; and finally, adding an additive combination into the oocyte maturation liquid according to the screened optimal dosage, detecting the first polar body discharge rate, the cleavage rate and the blastocyst rate to determine an optimal composition, and detecting the levels of ROS, GSH, mitochondrial membrane potential, oxidative stress and apoptosis related genes of the optimal composition to further determine the application effect of the composition.

The invention discloses an application of an Odf4 gene or protein as a biomarker in evaluating the ability of sperms to bind to oocytes. According to the invention, the interaction between Odf4 protein and sperm-egg binding key factors ZP3R and ADAM2 is found for the first time through experiments, and a sperm-egg recognition complex is formed; odf4 gene deletion can lead to significant reduction of the ability of sperms to bind to oocytes, and the formation rates of 2-cells, 4-cells, 8-cells and blastocysts of fertilized embryos are significantly reduced. On the basis, by detecting the expression level or functional integrity of the ODF4 in the sperms, the binding capacity of the sperms and oocytes and the development potential of fertilized embryos can be objectively evaluated. According to the invention, the limitation of low detection rate of recessive sperm-egg binding defects in the prior art is overcome, and an efficient molecular tool is provided for etiological typing, assisted reproduction scheme selection and fertility prediction of male infertility.

The present discloses provides the use of tributyrin as an additive for embryonic development culture medium in vitro, in the present disclosure, the tributyrin is applied as an additive to the development culture medium of mouse embryos in vitro for the first time, the tributyrin can significantly increase the rate of blastocyst, and reduce the ROS content in the embryos, improve the mitochondrial membrane potential in the embryos, increase the ATP level and the expression of antioxidant genes in the embryos, improve the DNA methylation and histone modification level in the embryos, promote the embryos development in vitro; In addition, tributyrin, as natural antioxidant and apparent drug, which is safe, non-toxic and side effects; the tributyrin provides strong support for the efficient embryos development of human assisted reproductive technology, mammalian fertilization embryos, parthenogenetic embryos and somatic cell cloned embryos and other embryo engineering technologies in vitro.

The invention discloses application of a compound shown in a formula I in promoting in-vitro cultured oocyte development or in-vitro embryo development, and in the formula I, R1 is selected from halogenated alkyl; r2 is selected from H, halogen or C1-6 alkyl; n is selected from 0, 1, 2 or 3. According to the application, the expansion area of cumulus cells in the cumulus-oocyte complex can be increased; the oxidative damage of the cumulus-oocyte complex is relieved; the first polar body discharge rate of the oocytes is improved; the abnormal distribution rate of mitochondria in oocytes is reduced; the membrane potential of mitochondria in the oocyte is improved; and the cleavage rate and blastocyst formation rate of fertilized eggs can be improved, and the apoptosis rate of oocytes can be reduced.

The present invention provides a primer set and method for analyzing the nanos1 gene of Macrobrachium rosenbergii, relating to the biotechnology field of reproductive-related genes in Macrobrachium rosenbergii. The Nanos1 gene obtained by the present invention has a cDNA sequence of 2811 base pairs (bp) long, encoding 243 amino acids. Nanos1 is specifically expressed in the ovary, and the expression level of nanso1 mRNA is highest in unfertilized eggs, significantly higher than after fertilization and at all stages of embryonic development. During embryonic development, expression is highest during fertilization, significantly higher than during the cleavage stage and extremely significantly higher than during the late embryonic development stage. The gene's expression level during the cleavage stage is significantly higher than from the blastocyst stage to the larvae stage; however, the expression level is lower and does not differ between the blastocyst stage and the larvae stage. Nanos1 mRNA is expressed in the cytoplasm of oogonia and primary oocytes (Oc1, Oc2, Oc3, and Oc4). Nanos1 is closely related to the development of female germ cells in Macrobrachium rosenbergii and plays an important role in the reproductive development of Macrobrachium rosenbergii, providing a theoretical basis and ideas for research on reproductive development and sex identification in Macrobrachium rosenbergii.

An apparatus configured to facilitate delivery of an embryo to an implantation site on an endometrium of a patient is provided. The apparatus comprises one or more walls defining a cavity, the walls comprising a rim configured for adhering to the endometrium and defining an opening to the cavity. The walls comprise one or more throughgoing apertures, each sized to allow passage therethrough of intrauterine environmental molecules, and to prevent passage therethrough of a blastocyst. The material of the walls is configured to degrade in the intrauterine environment within a predetermined range of time.

A double-walled embryo tube, a double-walled container and a gas-controlled non-return flip cap. The double-walled container is blow molded from the double-walled embryo tube. The double-walled bottle includes a bottle mouth, an inner bottle, an outer bottle and a gas pressure space therebetween. The gas-controlled non-return flip cap is combined with the bottle mouth. The gas-controlled non-return flip cap includes a valve and an air inlet. The gas-controlled non-return flip cap has a continuous air flow path with the double-walled container. When the outer bottle is squeezed, the air pressure in the gas pressure space forces the valve to close the air inlet, preventing air from escaping, so that the air pressure is used to press the inner bottle, causing the inner bottle to deform and release its contents from the gas-controlled non-return flip cap. When the squeezing force on the outer bottle is released, the valve is released from the air inlet, and external air flows into the gas pressure space through the air inlet, maintaining the air pressure in the gas pressure space, and the outer bottle returns to its original shape, and the inner bottle remains deformed. In addition, the gas-controlled non-return flip cap can prevent air from entering the inner bottle, reducing the risk of contamination or oxidation of the contents.

The invention provides an early pregnancy embryo ultrasonic maximum measurement section automatic grabbing system based on a real-time dynamic image, and the system comprises a multi-classification semantic segmentation module which is used for recognizing and segmenting a gestation sac, a yolk sac and a germ in an early pregnancy embryo ultrasonic dynamic image, and outputting a segmentation result; the measurement section capturing module is used for dynamically maintaining the longest diameter table of each category in the segmentation result based on the segmentation result, and determining the maximum measurement section of each category in the segmentation result according to a dynamic updating strategy; and the automatic measurement module is used for automatically measuring the maximum measurement tangent plane and generating a measurement result. The real-time performance and the stability of the system are improved, the measurement speed is remarkably improved, the consistency and the accuracy of diagnosis results are improved, and measurement errors caused by experience differences of doctors are reduced.

Provided are a reagent combination or a kit for constructing an embryoid and the use thereof. The reagent combination or the kit can be used for inducing a stem cell to produce a blastocyst-like lineage precursor cell, a blastocyst-like cell and / or an embryoid, which are based on small-molecule induction and independent of transgenes, and have a single cell source. The produced blastocyst-like lineage precursor cell and blastocyst-like cell have a full blastocyst lineage, and are seed cells having balanced developmental competence and totipotency, which solves the problem of unbalanced cell maturation in the prior art. The produced embryoid is highly similar to natural embryos in terms of morphological characteristics and transcriptomic characteristics, has the capability of highly reproducing post-implantation embryonic development and / or gastrulation, which is beneficial for research on embryonic development in vitro. The method for inducing the stem cell to produce the blastocyst-like lineage precursor cell, the blastocyst-like cell and / or the embryoid by using the reagent combination or the kit is simple, and has high applicability and high embryoid construction efficiency.

A stem cell-derived gastruloid model, its construction method, and its use are provided. The gastruloid model is constructed in vitro from human pluripotent stem cells and mimics, to some extent, biological events and major embryonic structures of early embryonic development, such as the development of primitive endoderm, the establishment of a bilayered blastocyst, the emergence of the amniotic cavity and amniotic cells, and the emergence of the primitive streak. It has been verified at both the protein and transcriptome levels. This model can better reproduce the major characteristics of the embryo from the peri-implantation stage to the gastrula stage. Furthermore, this model can be induced in batches for use as a screening model for drugs and environmental toxicants in early embryos, thereby providing safety testing for drug administration in specific clinical early pregnancy patients.

The invention discloses a sheep oocyte in-vitro maturation culture solution and a culture method. The sheep oocyte in-vitro maturation culture solution comprises an M199 culture medium, and fetal calf serum with a volume fraction of 10%, a penicillin-streptomycin mixed solution with a volume fraction of 1%, a follicle-stimulating hormone with a volume fraction of 0.02 IU / mL, a luteinizing hormone with a volume fraction of 0.02 IU / mL, 17 beta-estradiol with a volume fraction of 1 [mu] g / mL, cysteine with a volume fraction of 0.1 mmol / L and 13 (S)-HODE with a volume fraction of 200 nmol / L are added into the M199 culture medium. The sheep oocyte in-vitro maturation culture solution contains 13 (S)-HODE, and 13 (S)-HODE can reduce the lipid peroxidation level of sheep oocytes, improve the mitochondrial membrane potential, reduce the oocyte iron ion content, reduce oocyte ferroptosis and improve the blastocyst efficiency after oocyte in-vitro fertilization, can effectively improve the oocyte quality, and can be used for preparing the sheep oocyte in-vitro maturation culture solution. The early embryo development capability is improved.

The invention discloses a method for preparing heart organoid, and relates to the technical field of organ preparation, and the method comprises the following specific steps: S1, stem cell amplification and homogenized embryoid body preparation; s2, specific induction of cardiac progenitor cells: culturing the embryoid body, driving the cells to differentiate to mesoderm, and culturing again to obtain progenitor cells; s3, cardiac field zoning and multi-lineage spontaneous differentiation: putting the progenitor cells into a culture system to induce spontaneous differentiation of the cells; s4, forming a three-dimensional self-assembly and cavity structure: transferring the differentiated tissue into a dynamic rotary culture system to form a layered organ; s5, electrophysiology maturation and function strengthening, wherein the obtained organoid is subjected to programmed electric pulse stimulation, and the highly-bionic heart organoid is obtained. Through a staged and multi-dimensional induction strategy, by simulating the time sequence and space microenvironment of human embryo heart development, stem cells are guided to form the organoid in a self-organizing manner, and the use universality of the prepared heart organoid is improved.

The application discloses a method and system for preventing and controlling tuna fertilized egg overflow and biological predation, comprising: obtaining underwater real-time monitoring information, performing target detection based on the underwater real-time monitoring information to obtain target detection information; identifying the detection target based on the target detection information to identify yellowfin tuna and fertilized eggs and obtain underwater target identification information; performing target tracking according to the underwater target identification information, analyzing the movement path of the yellowfin tuna and the fertilized eggs in a unit time to obtain movement path analysis information; constructing a water flow field simulation model, analyzing the water flow field condition in the target aquaculture net cage, and performing fertilized egg movement trajectory prediction to obtain fertilized egg movement trajectory prediction information; judging whether the target fertilized egg is at risk based on the fertilized egg movement trajectory prediction information, generating risk warning information and formulating a control scheme. The yield and quality of tuna culture are effectively improved.

The invention provides a neural crest cell carrying a specific head-tail axis marker gene and a preparation method and application thereof, and relates to the technical field of biology, and the preparation method comprises the following steps: culturing stem cells to form an embryoid body; performing nerve induced differentiation on the embryoid body under the gradient driving of morphogenetic factors to form neural crest cells carrying specific head-tail axis marker genes, and promoting the formation of a neural tube-like structure with the polarity of the head-tail axis and the dorsal-abdominal axis when the embryoid body enters the nerve induced differentiation beginning by the gradient driving of morphogenetic factors. And inducing to generate neural crest cells carrying position-specific marker genes. The technical problem that neural crest cells carrying specific head-tail axis marker genes cannot be formed by an induced differentiation method in the prior art is solved.

The present invention provides a method for improving the efficiency of bovine in vitro embryo preparation, comprising the steps of in vitro collection of bovine oocytes, in vitro maturation and culture of cumulus-oocyte complexes, in vitro fertilization of bovine oocytes, and in vitro development and culture. The culture medium used in the in vitro development and culture contains 0.01-70 μM C 35 H 49 F2N7O4·CF3CO2H (MM102). By selecting the optimal bovine IVF system under in vitro conditions and placing the blastomeres in development medium supplemented with varying concentrations of MM102 after 48 hours of IVF development, the results showed that MM102 promoted the in vitro development of bovine zygotes, resulting in a high blastocyst rate. This method can be used to improve the efficiency of bovine IVF embryo preparation. This invention can provide a theoretical basis for research on livestock IVF technology and can be further applied to livestock breeding and genetic conservation.