7 results about "Immature oocyte" patented technology

The invention belongs to the technical field of nucleus visual identification, and discloses a method for identifying and predicting the nucleus position of an oocyte by using a computer image, which comprises the following steps of: dividing the oocyte into a mature oocyte and an immature oocyte, and acquiring an oocyte image; dividing the oocyte image into a mature oocyte image and an immature oocyte image; carrying out visual imaging operation on the nucleus of the oocyte, and meanwhile, carrying out image annotation on an oocyte photo; performing data amplification on images containing mature oocytes and cell nucleus positions, and dividing the images into a training set and a verification set; a computer vision framework is trained using the data set. According to the method disclosed by the invention, the cell nucleus position of the mammal oocyte is predicted by utilizing a computer visual identification technology, the enucleation accuracy is improved from 53.84% to 92.30%, and the accuracy of judging the cell nucleus position of the oocyte in a somatic cell nucleus transplantation process is improved.

The application relates to the field of super-low-temperature cryopreservation of mammalian oocytes, and particularly relates to application of miR-32 in improving the development capacity of pig immature oocytes after freezing. Pig immature oocytes are frozen and cultured in vitro, and mature oocytes which are cultured to expel the first polar body are incubated in transfection liquid containing a miR-32 nucleotide sequence for 2-6 hours; the pig immature oocytes are GV stage oocytes; and the miR-32 nucleotide sequence is shown in SEQ ID NO:1. It is found that miR-32 can be used as a key marker molecule of damage of vitrification oocytes, and by transfecting miR-32 mimics into the frozen oocytes, the early embryo development capacity of the oocytes can be improved, a new solution is provided for improving the maturation quality of the frozen oocytes, and various embryo biological technology researches are facilitated.

The present invention relates to the field of biotechnology and provides a composition, culture medium, product, and method for promoting in vitro maturation of immature oocytes. The composition comprises KT5823 and gastrodin. The composition can reduce the levels of pro-inflammatory factors in oocytes, alleviating the toxic effects of inflammation and autophagy on oocytes. Furthermore, it can effectively reduce reactive oxygen species (ROS) levels and increase the antioxidant glutathione content, thereby alleviating oxidative stress damage to oocytes. Through these synergistic effects, the composition significantly reduces oocyte apoptosis, significantly improves the quality of in vitro oocyte maturation, and significantly increases the development rate after in vitro fertilization.

The invention discloses a library building method for detecting G-quadruplex structures in oocytes, early embryos and trace cells, and belongs to the technical field of whole genome immunoprecipitation library building. The DNA G-quadruplex structure provided by the invention is a DNA secondary structure which is different from a classic double-helix structure; g-quadruplex structure abnormity can damage telomere stability, genome stability, transcriptional activity and the like; this is an important reason for poor ovum quality and embryonic development retardation. The method provided by the invention can be used for evaluating the quality of the ova of the infertile women and judging reasons causing immaturity of the ova; abnormal enrichment of in-vivo and in-vitro development arrest embryo whole genome G-quadruplex is detected and can be used as an important index for screening reasons causing embryonic development retardation; the method can be used for guiding whether corresponding clinical patients are suitable for a series of assisted reproductive technologies (ART) such as in vitro fertilization-embryo transfer (IVF-ET) and the like.

A method for inducing immature oocytes includes introducing four genes consisting of FIGLA, NOBOX, LHX8 and TBPL2, or transcripts or expressed proteins thereof, into at least one type of cell selected from the group consisting of pluripotent stem cells, epiblast-like cells and primordial germ cells. A method for producing mature oocytes includes introducing four genes consisting of FIGLA, NOBOX, LHX8 and TBPL2, or transcripts or expressed proteins thereof, into at least one type of cell selected from the group consisting of pluripotent stem cells, epiblast-like cells and primordial germ cells, and co-culturing the cell obtained after the introduction and ovarian somatic cells.

The invention relates to the technical field of assisted reproduction, and discloses an in-vitro maturation culture system and method for immature oocytes of elderly women based on NMN supplement. The culture system is an in vitro maturing (IVM) culture solution containing 10 [mu] M of NMN (Nitrogen Morphocyte Nitride), and the culture system is an in vitro maturing (IVM) culture solution containing 10 [mu] M of NMN; the culture method comprises the following steps: preparing an IVM culture solution; culturing in vitro; carrying out in-vitro fertilization; and early embryo development observation. According to the application, NAD < + > is improved through targeted supplement of NMN, the mitochondrial function is further repaired, a brand new path for cooperatively regulating meiosis is achieved, and the problem that in the prior art, only oxidative stress is relieved, but the energy supply defect cannot be solved is solved. Experimental data show that the IVM system added with the NMN can remarkably improve the blastocyst formation rate of oocytes of elderly women, the mitochondrial function is recovered, and the NMN-containing IVM system has a remarkable clinical transformation value.

The invention discloses an efficient immature oocyte microinjection method which comprises the following steps: sucking an oocyte washing liquid by using a pipettor, adding the oocyte washing liquid into a culture dish until the oocyte washing liquid submerges the bottom of the culture dish, then adding immature oocytes, aligning a cumulus oocyte complex in the culture dish by using the pipettor, and gently blowing and beating to remove part of cumulus, the method comprises the following steps: sucking one oocyte by using a common oocyte transfer needle, placing the oocyte in a T10 liquid drop, pressing a part of a residual cumulus cell by using a small-caliber oocyte transfer needle, gently and repeatedly sucking and blowing until part of oocyte zona pellucida is exposed, transferring the oocyte zona pellucida into another T10 liquid drop, and cleaning; the exposed zona pellucida part of the oocyte is aligned with the microinjection needle, and when the needle tip of the microinjection needle is observed to be tangent to the spherical surface of the oocyte under a microscope, the needle tip is inserted into cytoplasm from the exposed zona pellucida part to inject siRNA. According to the invention, efficient injection of immature oocytes can be realized, and meanwhile, the influence on maturation of the oocytes is smaller.