46 results about "First polar body" patented technology

A full set of 24 chromosome-specific probes to analyze single cells or cell organelles to test for abnormalities is described. When used in an assay based on sequential hybridization, the full set is comprised of three subsets of chromosome-specific probes with each set comprised of 8 different probes. Also described are assays using a set of probes to analyze single cells and cellular organelles to accurately determine the number and type of targeted human chromosomes in various types of cells and cell organelles, such as tumor cells, interphase cells and first polar bodies biopsied from non-inseminated oocytes. Methods of selection or generation of suitable probes and hybridization protocols are described, as are preferred probes for frill set of 24 chromosome-specific probes to target all 24 human chromosomes are described in the Tables.

The invention relates to a method for improving in-vitro production efficiency of buffalo embryos, comprising the following steps of: respectively adding 10 muL / mL of insulin-transferrin-selenium (ITS) and 20 muL / mL of insulin-transferrin-selenium (ITS) to an oocyte maturing solution and a culture solution, and then carrying out in-vitro maturation and in-vitro culture. The method indicates that the in-vitro maturation rate of buffalo oocyte is effectively improved by adding 10 muL / mL ITS to the maturing solution, and the exhaustion ratio of a first polar body is up to 65.61%; and adding 20 muL / mL of ITS to the embryo culture solution is beneficial to the growth of early embryos of a fertilized buffalo, and the growing rate of a blastaea is up to 29.93%. the method has the advantages of improving the in-vitro maturation rate of the buffalo oocyte and the growing rate of the blastaea, thereby improving the in-vitro production efficiency of the buffalo embryos and providing a large number of high-quality embryos for embryo transplantation.

The invention discloses a method for inducing ocean seashell polyploidy by using sodium chloride. The method is characterized by comprising the following steps: selecting the seashell to grow the shell to respectively obtain sperms and ovum; carrying out artificial insemination; continuously observing the developing condition of zygotes through microscopic examination to determine the number of the water body for culturing the zygotes; adding sodium chloride into the culture sea water containing the zygotes according to a proportion that 20-40 go of sodium chloride is added in 1000ml of culture seat water when 40-50 percent of zygotes are observed to release a first polar body or a first zygote is observed to occur the first polar body, so as to prevent the release of a second polar body or the first polar body of the zygotes; evenly stirring and treating for 10-15 min; and transferring the zygotes to normal sea water to incubate . The ocean seashell comprises Pacific oyster, chlamys farreri, Argopecten irradians, Crassostrea rivularis, clam, Scapharca broughtonii and the like. The seashell zygotes are treated by using the sodium chloride and the initiating agent so as to inhibit the release of the polar body and produce the shell polyploidy with no toxicity, efficiency, simple operation and extremely low cost.

The invention provides a cultivation method for rapidly growing triploid crassostrea gigas Haida No.1. The method comprises the following steps: obtaining high-heterozygosity triploid crassostrea gigas Haida No.1 by inhibiting discharge of first polar bodies of fertilized eggs of diploid crassostrea gigas Haida No.1, taking females of the triploid crassostrea gigas Haida No.1 as female parents, taking males of the diploid Haida No.1 as male parents, and obtaining tetraploid crassostrea gigas Haida No.1 with improved growth characters by inhibiting discharge of the first polar bodies of the fertilized eggs; and hybridizing males of the tetraploid crassostrea gigas Haida No.1 and the diploid crassostrea gigas Haida No.1 to obtain the triploid crassostrea gigas Haida No.1 which grows quickly. According to the method, the growth performance of the crassostrea gigas is improved by adopting a method for improving the heterozygosity of parent genes, and the tetraploid crassostrea gigas Haida No.1 obtained by adopting the method is hybridized with the diploid crassostrea gigas Haida No.1 which originally has the characteristic of rapid growth to obtain the triploid crassostrea gigas Haida No.1 with rapid growth performance.

The invention belongs to the technical field of genetic breeding of aquaculture varieties, and relates to a method for producing tetraploid oysters and a method for interspecific hybridization of triploid oysters by using the tetraploid oysters. The triploid oysters are stimulated for 5-8 days at the high temperature of 26-28 DEG C, ripening is promoted for 30-40 days at the water temperature of 22-24 DEG C after stimulation, and ova of female oysters with the largest diameter are selected after ripening; the selected ova and sperms of diploid oysters are fertilized for 5-8 minutes, then an inhibitor is added to inhibit first polar body discharge of the fertilized ova, and then a large number of tetraploid oysters are stably obtained for optimization. After strong optimization, high-concentration sperms are used for carrying out hybrid fertilization on excellent tetraploid oysters and excellent diploid oysters in different species, and interspecific hybrid triploid oysters with excellent breeding traits are obtained. According to the method for producing the tetraploid oysters and the method for interspecific hybridization of the triploid oysters by using the tetraploid oysters, the method for producing the tetraploid oysters and the method for interspecific hybridization of the triploid oysters by using the tetraploid oysters can be used for stably and efficiently producing the tetraploid oysters and the hybrid triploid oysters with the excellent breeding traits, can be widely applied to genetic breeding of oysters and shellfishes, and improves the shellfish breeding industry.

The invention relates to a method for producing viable tetraploid bivalve molluscs, by fertilisation of diploid female ovocytes with diploid male sperm, followed by the induction of the retention of the first polar body of the fertilised eggs, isolation from the obtained larvae of a larval sub-population enriched in tetraploids and selective raising of said sub-population.

The invention discloses a preparation method of young mollusks of Hongkong tetraploid oysters. The method comprises the following technical steps of selectively choosing triploid eggs, carefully choosing diploid sperms, inhibiting second polar bodies of oosperms, obtaining tetraploid young mollusks, and raising tetraploid progenies. The feature of normal reduction mitosis of some triploid eggs is adopted so as to create a raising method for preparing tetraploid oysters by inhibiting second polar bodies of oosperms (triploid eggs +diploid sperms). The method differs from a method for obtaining tetraploid oysters by inhibiting first polar bodies of oosperms. The preparation method makes a great difference in a traditional preparation mode and helps break the monopolies of the US, France, Korea and other foreign countries on raisins technology of tetraploid oysters. It is the first time to obtain livable young mollusks of Hongkong tetraploid oysters. The method makes the biological method of producing 100% Hongkong triploid oysters feasible, promote the steady development of the industry of Hongkong oysters in the coastal region of southern China and offers the possibility of achieving improved varieties of the industry. The preparation method of young mollusks of Hongkong tetraploid oysters is being advantaged by being implementable, reusable, and applicable.

The invention discloses a time point quantification treatment method for producing a Crassostrea hongkongensis all-triploid. The time point quantification treatment method in which a fertilized egg development stage is taken as a biological indicator is created through the technical links of collection of single fertilized eggs, timely treatment of the fertilized eggs, detection of larva ploidy and the like, so that a 100-percent Crassostrea hongkongensis all-triploid can be obtained. Through optimization and pairing of the single fertilized eggs, the defect of poor mixed fertilized egg synchronicity in a conventional Crassostrea hongkongensis triploid induction process is overcome effectively, and zygotes with high synchronicity are obtained. A conventional fixed thinking mode in which a time point when 30-50 percent of a first polar body occurs is taken as a treatment time point and the optimal time interval is 20 minutes is overturned. Instead, the fertilized egg development stage is taken as the biological indicator, and A+1 / 3B and B+C are taken as a treatment time point and a treatment time interval respectively, so that the influences of external environmental conditions such as temperature on treatment are avoided, and the Crassostrea hongkongensis all-triploid can be induced successfully.

The invention discloses a method for in-vitro differentiation from induced pluripotent stem cells to oocyte stagnating in the meiosis II stage. The method includes the three steps of firstly, puttingpluripotent stem cells with the cell density of 80% in a PGC-m culture medium to be cultured for 10 days at 35-40 DEG C in CO2 with the concentration of 3-6% to obtain original germ cell-like cells; secondly, transferring the formed original germ cell-like cells into a PF-m culture medium to be cultured for 5 days at 35-40 DEG C in CO2 with the concentration of 8-12% to obtain a follicle-like structure; finally, transferring the formed follicle-like structure into an OLC-m culture medium to be incubated for 10-15 days to obtain oocyte-like cells stagnating in the meiosis II stage. The method is high in efficiency and short in time, and the secondary oocyte can be formed by removing first polar bodies from the induced oocyte-like cells. The method provides new means for studying the formation of human female germ cells and the generation of ova.

The invention discloses an induction method for a haliotis discus hanai triploid, and relates to a shellfish culture method. The method comprises the steps of selecting abalone seeds and promoting maturity; performing artificial spawning induction; selecting and processing gametes; performing synchronous fertilization; performing medicine induction; removing medicine; performing incubation. On thepremise of ensuring haliotis discus hanai seed gonad maturity and synchronous fertilization, the induction condition is further optimized, and therefore the haliotis discus hanai triploid is obtainedefficiently and stably. Synchronous fertilization is ensured, the phenomenon that gametes are in contact in advance in the discharge process is strictly avoided, microscopic examinations determine that no accident fertilization exists, and the consistency of synchronous fertilization and germ cell development is ensured. The induction conditions are optimized, wherein constant microscopic examination observation determines that 70-80% of contrast group germ cells PB1 are adopted as a processing time node, it is ensured that a first polar body is effectively released, and tetraploids and aneuploids are avoided. According to the stable and efficient induction rate, the induction rate of the triploid is 100% or is close to 100%.

The invention discloses a method for chemically inducing an abalone allotriploid and relates to a method for cultivating shellfish. The method comprises the steps: (1) semen abalone selection and maturation promotion; (2) artificial spawning induction; (3) gamete selection and processing; (4) synchronous fertilization; (5) drug induction; (6) drug removal; (7) hatching. Synchronous fertilization is ensured: gametes are strictly prevented from being in contact in advance in the discharging process, it is confirmed through microscopic examination that accidental fertilization does not occur, and the consistency of synchronous fertilization and fertilized egg development is guaranteed. Induction conditions are optimized: 70%-80% of the PB1 proportion of fertilized eggs in a control group is determined as a processing time node by constant microscopic observation, it is ensured that the first polar body is effectively released, and a tetraploid and an aneuploidy are avoided. The stable and efficient induction rate is that the triploid induction rate is 100% or close to 100%.

The invention discloses a method for cultivating a pearl by inducing triploid hyriopsis cumingii. The method comprises the following steps of: cultivating the triploid hyriopsis cumingii by induction, namely selecting diploid hyriopsis cumingii with age of 3 to 6, shell length of over 15cm and shell thickness of over 5cm in normal growth as parent clams; isolating the male clams from the female clams of the selected parent clams for cultivation respectively; tracking and observing the development condition of the eggs of the female clams; transferring the female clams into a pond for cultivating the male clams to perform natural insemination on the female clams after the eggs are maturely developed; observing the development condition of the fertilized eggs; putting the female clams into 300 to 450 mu mol / L aqueous solution of 6-dimethyl aminopurine after over 80 percent of the fertilized eggs have first polar bodies; and performing induction processing for 10 to 30 minutes to obtain induced female clams; cultivating the female clams, and hatching and raising the seedlings to obtain the triploid juvenile clams; and performing cultivation to obtain the triploid hyriopsis cumingii. In the method, the normal diploid hyriopsis cumingii is used as the parents, and the natural insemination is adopted, so that the induction success rate is improved to over 70 percent.