298 results about "Oocyte" patented technology

Provided is an ease-to-operate automatic flushing structure, comprising a mounting housing (2), an injector driving mechanism, and an injector clamping mechanism. The mounting housing (2) is a structure with a clamping groove on a side portion thereof, and the injector clamping mechanism is arranged on an outer side of the clamping groove. The injector driving mechanism is arranged in the mounting housing (2). Also provided is an oocyte acquisition device, comprising an acquisition device body. An automatic flushing structure is arranged on a side portion of the acquisition device body.

The invention discloses a traditional Chinese medicine composition for repairing and / or activating residual ovarian functions of an aging ovary, and a preparation method and application thereof, and relates to the technical field of biology. The traditional Chinese medicine composition is prepared from the following components: radix astragali, radix angelicae sinensis, radix ophiopogonis, radix polygonati officinalis, semen cuscutae, radix morindae officinalis, fructus rubi, rhizoma dioscoreae and folium mori. The invention further provides application of the traditional Chinese medicine composition in repairing and / or activating the residual ovarian function of the senescent ovary and improving the quality of the senescent oocyte, the traditional Chinese medicine composition can increase the volume and weight of the senescent ovary, increase the number of follicles of all levels in the senescent ovary, reduce apoptosis of granular cells in the ovary and improve the survival rate of the senescent ovary. The abnormal rate of oocytes, oxidative stress of the oocytes and DNA damage are reduced, development and maturation of residual follicles are promoted and maintained, and the traditional Chinese medicine composition has a wide application prospect.

The invention provides an ovary-targeting ROS response type SS31 nano-particle and a preparation method and application thereof, and belongs to the field of biomedicine.The ovary-targeting ROS response type SS31 nano-particle is constructed by utilizing a porous metal organic framework to carry hyaluronic acid and utilizing an ROS response type thioketone linker to be combined with mitochondrial targeting peptide SS31, and the ovary-targeting ROS response type SS31 nano-particle is used for preparing the ovary-targeting ROS response type SS31 nano-particle. And the SS31 is targeted and delivered to the ovarian tissue. The nanoparticles target ovary and slowly release SS31 under the action of ROS, so that oxidative stress of oocytes and granular cells caused by female reproductive system diseases such as premature ovarian failure, polycystic ovarian syndrome and the like is reduced, and the mitochondrial function of the oocytes and the granular cells is improved; and a foundation is laid for the subsequent clinical treatment of diseases related to the mitochondrial dysfunction of the female reproductive system by using the SS31.

The invention provides an application of a CTH inhibitor and / or an xCT inhibitor in preparation of a medicine for inhibiting mammal embryo implantation, and relates to the technical field of medicines. The application effect of a cystathionine-gamma-lyase (CTH) inhibitor and / or a solute carrier family 7 member 11 (xCT) inhibitor in inhibition of mammalian embryo implantation is verified through research. The research result shows that the xCT inhibitor and / or the CTH inhibitor can obviously inhibit proliferation of corpus luteinized granular cells and secretion of progesterone of a model mouse; meanwhile, by injecting the xCT inhibitor and / or the CTH inhibitor into the mouse model in vivo, ovary ovulation and the maturation rate of the ovulated oocyte nucleus can be remarkably inhibited, functional corpus luteum formation and embryo implantation can be remarkably reduced, and then a new thought and method are provided for development of contraceptive drugs.

The invention relates to the technical field of assisted reproduction polygene detection, and discloses an oocyte occurrence and maturation disorder detection panel, a detection kit and application thereof, and the detection panel comprises mutation genes related to the oocyte occurrence and maturation disorder for detection, copy number variation genes and rearrangement event and deletion genes. According to the application, by detecting high-risk genes and mutation sites, the risk of occurrence and maturation disorder of the oocytes is predicted in combination with clinic, the development potential of the oocytes is evaluated, the in-vitro maturation strategy is optimized, and the clinical outcome of assisted reproduction is improved. The detection panel can be used for efficiently detecting gene mutation which has clinical diagnosis and treatment significance on oocyte occurrence and maturation disorders; the method has important guiding significance on genetic counseling, risk prediction, clinical diagnosis, precise treatment and potential personalized intervention measures of patients suffering from recurrent oocyte occurrence disorder, oocyte maturation disorder and low oocyte maturation rate and patients to be subjected to assisted reproduction technology treatment.

The present invention discloses the application of MY-1B in the preparation of drugs for improving ovarian senescence, belonging to the technical field of biomedicine, aiming to solve the problem of how to effectively delay ovarian senescence and improve ovarian function. Experiments have proved that during ovarian senescence, the mRNA m<supgt;5< / supgt;C modification level and the expression level of methyltransferase in ovarian granulosa cells increase. Supplementation with MY-1B can improve the condition caused by mRNA m Nsun2 expression.

A method of analysing a sample of a plurality of fish oocytes (100) is disclosed The method comprises, for each oocyte in the sample: capturing a first set of images of the oocyte from a plurality of rotational angels when rotated about a first axis of rotation; capturing a second set of images of the oocyte from a plurality of rotational angels when rotated about a second axis of rotation, which second axis of rotation is orthogonal to the first axis of rotation; identifying at least one characteristic (104) of lipid droplet coalescence in the captured first and second sets of images; and based on the identified at least one characteristic of lipid droplet coalescence, classifying the oocyte as belonging to one of a plurality of predefined categories, and based on the distribution of the sampled oocytes in the plurality of categories, attributing a quality parameter to the sample of oocytes, the quality parameter being any one of: a predicted fertilisation rate of the sampled oocytes; a predicted eyed-embryo success rate of the sampled oocytes; and a predicted hatching success rate of the sampled oocytes. A computer-implemented method of attributing a quality measure to a sample of fish oocytes is also disclosed, as is a related computer program and a related oocyte analysis apparatus. A computer-implemented method for training a neural network for use in a system for analysing a sample of fish oocytes is also disclosed.

The invention relates to a method for electrically activating oocytes based on nanosecond pulses. Single oocytes are sequentially and uniformly arranged between two parallel electrodes of an electrode dish, the electrode dish is characterized in that the distance between the electrodes is 1 mm, the electrodes are placed in a culture dish, and the two ends of the two electrodes are sealed; a culture solution is firstly put between the electrodes which are arranged in parallel, mineral oil is covered to prevent the culture solution from being evaporated, after preheating treatment, oocytes are sequentially and uniformly put between the two electrodes which are arranged in parallel one by one, and then nanosecond pulse electric activation treatment is applied. The electrodes which are arranged in parallel are adopted, the oocytes are sequentially and uniformly placed between the electrodes in a single mode, the situation that the electric pulse effect is reduced due to adhesion and stacking of all the cells is avoided, the oocytes receive uniform and equivalent electric pulse sequences, the action effect and stability of electric pulses on the cells are obviously improved, and the operation efficiency of the oocytes is improved. And the reliability and reproducibility of a test conclusion are improved.

The invention discloses application of rapamycin combined with a vascular endothelial growth factor A in improvement of an ovarian tissue function. The improvement of the ovarian tissue function comprises any one or more than two of the following aspects: increasing the survival rate of follicles, prolonging the functional life of the ovarian tissue and improving the quality of oocytes. The effective concentration of the rapamycin is 500nM, and the effective concentration of the vascular endothelial growth factor A is 5ng / mL. The follicle loss dynamic after OTCT is tracked through a mouse model, it is found that rapid follicle loss is related to tip cell damage and primordial follicle activation, then a rapamycin and vascular endothelial growth factor A combination strategy is provided, follicle loss can be remarkably reduced, the reproductive function period can be prolonged, and the oocyte quality can be improved; according to the invention, a systematic solution is provided for OTCT technology optimization.

The invention discloses application of an ACSL4 inhibitor in preparation of a medicine for improving quality reduction of an elderly ovum, and belongs to the technical field of biological medicines. The invention provides an application of targeting long-chain fatty acid acyl-coenzyme A synthetase 4 (ACSL4) in intervention of low oocyte quality and related fertility decline caused by aging. The ACSL4 inhibitor can be used for preparing drugs for improving ovarian functions and improving oocyte quality. In-vivo and in-vitro experiments prove that by inhibiting the ACSL4, the aging process of the elderly ovary can be effectively relieved, the expression of aging-related proteins p16 and p21 can be reduced, the mitochondrial function of the oocyte can be improved, and spindle assembly abnormality and chromosome arrangement defects can be rescued, so that the maturation rate and the development potential of the oocyte can be remarkably improved.

The invention discloses a bovine oocyte in-vitro maturation culture solution as well as a preparation method and application thereof, and belongs to the technical field of animal reproduction and embryo engineering. The preparation method of the bovine oocyte in-vitro maturation culture solution comprises the step of adding mitoquinine mesylate into a conventional IVM culture solution, wherein the adding concentration of the mitoquinine mesylate is preferably 50 nmol / L. The bovine oocyte in-vitro maturation culture solution provided by the invention can effectively reduce the oxidative stress reaction of the oocytes in the in-vitro culture process, enhance the mitochondrial function and remarkably improve the bovine oocyte in-vitro maturation quality and the embryonic development potential, so that the blastocyst rate is increased, and the problem that the development potential of the existing oocyte IVM culture solution is insufficient is solved.

The invention relates to the technical field of animal husbandry, in particular to a synchronous oestrus-timing insemination method suitable for cattle, which comprises the following steps: firstly, performing intramuscular injection of PG (cloprostenol sodium) at different time points after calving to promote uterine recovery and luteolysis and lay a synchronous oestrus foundation; the method comprises the key steps that GnRH (gonadotropin release hormone) is given through intramuscular injection on the 43rd day, the 53rd day, the 60th day and the 69th day, pituitary is promoted to release follicle-stimulating hormone (FSH) and luteinizing hormone (LH), collection and screening of follicles are guided, follicle maturation is promoted, the oestrus uncovering rate and the oestrus conception rate are increased, a melatonin implant is implanted in a subcutaneous implantation mode on the 63rd day, and the melatonin implant is implanted in a subcutaneous implantation mode on the 63rd day. The synthesis of ovarian hormones is promoted by regulating the hypothalamus-pituitary-gonad axis, the secretion of follicle cell factors and maturation promoting factors is promoted, the development of granular cells is promoted, and the maturation of oocytes is promoted. According to the method, the pregnancy rate of a double-synchronization-timing insemination scheme which is most widely used in a large-scale dairy cow breeding pasture at present can be remarkably increased, and feasible technical support is provided for success of increasing the breeding efficiency of cows.

This invention relates to the fields of molecular biology and biomedicine, and discloses siRNA for the expression of the *Schistosoma japonicum* Hsc20 gene and its applications. The siRNA contains a nucleotide sequence that hybridizes to a target sequence of the *Schistosoma japonicum* Hsc20 gene, wherein the target sequence is selected from the sequences shown in SEQ ID NO. 1-SEQ ID NO. 4. The siRNA specifically inhibiting the expression of the *Schistosoma japonicum* Hsc20 gene provided by this invention can be used to interfere with the transcription and expression of the *Schistosoma japonicum* Hsc20 gene and the growth and development of *Schistosoma japonicum*. In vitro experiments have confirmed that the siRNA provided by this invention can efficiently silence the *SjHsc20* gene; and in vivo RNA interference experiments in mice show that this siRNA can induce 50.62% (…) P The insect reduction rate was <0.05% and 44.29% ( P A liver oocyte reduction rate of <0.01% is suitable for preparing drugs to treat schistosomiasis.

The invention discloses a micro-fluidic chip for exploring in-vitro XY type sperm fertilization preference, and relates to the technical field of biomedical engineering.According to the technical scheme, the micro-fluidic chip is characterized by comprising a chip body, the chip body is provided with a central sample carrying hole, the central sample carrying hole is used for containing an oocyte, and the central sample carrying hole is communicated with the central sample carrying hole; the chip body is provided with four peripheral sample carrying holes, the four peripheral sample carrying holes are used for placing fluorescently-labeled sperm cells subjected to sexual control sorting, and communicated micro-channels are arranged between the central sample carrying hole and the four peripheral sample carrying holes. Observation is facilitated by improving the volume of a liquid carrying hole and optimizing the transparency of a material, meanwhile, by adjusting the diameter of a channel and the number of repeated units, the thickness of the micro-fluidic chip is reduced, micro-channel blockage is avoided, the number of cells in loaded liquid is guaranteed, and the detection accuracy of the micro-fluidic chip is improved. The preference of successful fertilization of X and Y type sperms under different conditions is observed in vitro through micro-fluidic, and a new thought is provided for a method for controlling the gender of a fetus.

The application discloses a kind of marker for predicting oocyte copy number variation and application thereof, the marker includes the combination of CLEC11A, P4HB, EFEMP2, IL32, FTL, FLNA, COL6A3, ACPP and APOO protein.By the marker of the application, the expression level of granulosa cell secretory protein can be determined by granulosa cell transcriptome sequencing, and the genomic CNV condition of corresponding oocyte can be inferred.The detection object of the technical solution is discarded granulosa cell in assisted reproductive technology, so it will not have any impact on oocyte, and it is non-invasive;The present study is a transcriptome and methylation group detection from single cell level, with high-throughput characteristics, and all granulosa cells and oocytes are one-to-one matched, which can reflect the difference between single cell level COCs.

The utility model discloses a flushing connecting device capable of efficiently operating and a double-cavity oocyte collector, the flushing connecting device comprises a flushing fluid suction tube, a hard connecting tube and a three-way connector, a second port of the flushing fluid suction tube is connected to the hard connecting tube, a first one-way valve is arranged at a first port of the three-way connector, and a second one-way valve is arranged at a second port of the three-way connector. A first port of the three-way connector is connected with a first port of a flushing fluid suction tube, a second port of the three-way connector is connected with an injector, and a third port of the three-way connector is provided with a second one-way valve and is connected with a flushing tube. The double-cavity oocyte collector comprises a double-cavity oocyte collecting needle tube, a washing tube, a liquid medicine filter and the washing connecting device, a first port of the washing tube is connected with a third port of the three-way connector, a second port of the washing tube is connected with a washing cavity of the double-cavity oocyte collecting needle tube, and the liquid medicine filter is arranged on the washing tube. The ovum extractor can be used more efficiently, the operation time can be remarkably saved, and the ovum extraction efficiency is improved.

An apparatus for diagnosing an embryo implantation potential using an artificial intelligence-based diagnostic model according to the present disclosure includes a memory storing a diagnostic model trained to determine an implantation potential index for an embryo based on an image generated by photographing one or more embryos formed by fertilization of a sperm and an oocyte and before implantation, and a processor for inputting a diagnostic image generated by photographing one or more first embryos, which are determination targets of the implantation potential index, into the diagnostic model as input data, and receiving implantation potential index data, which is a result of determining the implantation potential index for the first embryo, as output data from the diagnostic model.

The present invention relates to a medium composition for in vitro fertilization and / or in vitro culture comprising a compound represented by chemical formula 1 or a pharmaceutically acceptable salt thereof, and a method using same. The compound or composition suppresses reactive oxygen species and lipid peroxidation in ovarian granulosa cells to preventing cytotoxicity and mitochondrial dysfunction caused by apoptosis and ferroptosis, leading to an improvement in the quality of aged oocytes, and to increase the developmental rate and blastocyst formation rate of pre-implantation embryos, thereby improving the efficiency of in vitro fertilization. In addition, treatment with the medium composition during the vitrification process of in vitro–fertilized embryos improves re-expansion and survival rates, thereby reducing structural and metabolic damage. Accordingly, the present invention can be advantageously applied to an assisted reproductive technology for treating infertility and subfertility, and in vitro fertilization and / or in vitro culture for improving the propagation efficiency of livestock.

A microfluidic device that includes a trap configured to retain an oocyte or fertilized species, a plurality of microchannels in fluid communication with the trap, and a plurality of valves, where each valve is configured to control fluid flow through a corresponding set of one or more of the plurality of microchannels. The microfluidic device further includes a plurality of fluidic timer circuits each configured to produce a corresponding first time-dependent fluid pressure signal, and a fluidic logic gate circuit configured to receive the first time-dependent pressure signals and to produce a plurality of second time-dependent fluid pressure signals, where each second time-dependent fluid pressure signal controls operation of a corresponding one of the valves.

The invention discloses a method for promoting ovulation of sexual maturity zebrafish by using Gap27, and relates to the technical field of artificial breeding of fish, a gap connecting protein inhibitor Gap27 is applied to ovulation induction of zebrafish, an intercellular communication network in follicles is accurately intervened, the problem of asynchronous ovulation is solved, and in the final stage of maturation of oocytes, ovulation of sexual maturity zebrafish is promoted. Close substance and information exchange is carried out among follicular granule cells through a gap connecting channel formed by connecting protein Cx43, and Gap27 is used as a specific peptide fragment, can simulate an extracellular ring structure of Cx43, and competitively binds with Cx43 on an adjacent cell membrane, so that formation and functions of the gap connecting channel are physically blocked, and the follicular granule cells are separated from each other. When ovarian tissues of sexually mature female fish are exposed in a Gap27 solution, the transmission of inhibitory signals from granular cells to oocytes can be effectively interrupted, the rupture of nuclear membranes of the cells is promoted, and the final maturation process is completed.

The present invention relates to a double-lumen oocyte aspiration needle and use thereof in the collection of oocytes from the ovaries of animals undergoing assisted reproduction procedures.

The present invention relates to the field of biotechnology. Specifically, the present invention relates to a method for rapidly extracting the nuclei of germinal vesicle (GV)-stage oocytes and pronuclei of zygotes. This method uses a self-made egg transfer needle to aspirate oocytes or fertilized eggs, and then uses a self-made glass needle to aspirate the nuclei of GV-stage oocytes and pronuclei of zygotes from the nuclear extraction droplet. A relatively large number of nuclei of GV-stage oocytes or pronuclei of fertilized eggs can be obtained in a short time, and expensive equipment and professional technicians are not required. The obtained nuclei of GV-stage oocytes or pronuclei of fertilized eggs have high integrity and low contamination rate, and have great application value.

The invention discloses application of spermidine in in-vitro culture of human unfrozen oocytes and embryos. By adding the combination of spermidine, melatonin and astaxanthin into the thawed oocyte resuscitation culture solution and the embryo in-vitro culture solution, the mitochondrial autophagy and mitochondrial functions of the thawed oocytes are improved, so that the in-vitro culture effect of the thawed oocytes and embryos is improved, and the success rate of an assisted reproduction technology is increased.

The present application relates to the technical field of computer vision, and particularly relates to an oocyte maturity analysis method, device and computer storage medium, the analysis method comprises the following steps: S1, classifying oocyte images into oocyte images with granulosa cells removed and oocyte images without granulosa cells removed; further classifying into mature and immature; S2, data augmentation is performed on the immature; S3, the oocyte images are segmented into non-overlapping image blocks, through four-stage processing, the feature maps of each image block in multiple dimensions are obtained; the weight scores of the feature maps in different dimensions are calculated in the dimension direction; the probability value of the category to which the image to be predicted belongs is output through a full connection layer, and the analysis of oocyte maturity is completed; the method of the present application has higher recognition preparation rate, faster speed, stronger device mobility, and has a significant effect on recognizing oocyte images before granulosa cells are removed.

The first objective is to identify previously unknown factors or combinations of factors that can efficiently produce oocyte-like cells from pluripotent stem cells, etc. The second objective is to provide oocyte-like cells and an efficient method for producing them. [Solution] A method for producing egg-like cells, which includes a step of introducing a factor into a cell, wherein the factor includes DLX6 or a nucleic acid encoding DLX6, and egg-like cells derived from human pluripotent stem cells and expressing PADI6.

The invention is suitable for the technical field of insect genetic engineering, and provides an HparGR22 gene and application thereof in establishing a phlorizin recognition in-vitro model. The DNA sequence of the HparGR22 gene is as shown in SEQ ID No: 1, and the coded amino acid sequence is as shown in SEQ ID No: 2. The gene has the highest expression quantity in the lower labial tassel of a holotrichia parallela larva, an oocyte injected with cRNA of the gene has obvious response to stimulation of phlorizin with different concentrations, it is clear that a product expressed by the HparGR22 gene can recognize phlorizin, and the function of the HparGR22 gene as the phlorizin receptor gene of the holotrichia parallela is determined. In view of the fact that the phlorizin recognition function of the gene is clear for the first time, a basis is provided for application of the gene in establishment of a phlorizin recognition in-vitro model, a foundation is laid for exploring the potential value of the gene in the field of gene engineering for preventing and controlling holotrichia parallela, and meanwhile a new direction is opened up for further research and application of the gene.

An oocyte retrieval tubing system comprising a manifold for receiving a supply of heated air and an outer tubing for accommodating an internal transfer tube that extends between a collection container and an oocyte retrieval needle in use, the outer tubing comprising first and second separate insulating tubes connected to each other via a manifold, the first and second insulating tubes each configured to be adjustable in length, whereby adjusting their working lengths relative to each other regulates the flow of heated air within the outer tubing received through the manifold disposed therebetween to maintain a stable and uniform temperature distribution within the outer tubing for oocyte retrieval.

The utility model relates to a kind of cell vitrification freezing automatic systems, including workbench;Including several injection pumps, the injection pump is installed on workbench, movable piston rod is provided in the injection pump, and the piston rod upper end is connected with driving assembly;Including fluid collecting valve, the fluid collecting valve is installed on workbench, and the fluid collecting valve is connected with the liquid outlet end of injection pump;Including frozen macaroni, the frozen macaroni and fluid collecting valve are connected;Including temperature control board, the temperature control board is installed on workbench, the utility model is advantageous in that system automatically controls the loading and unloading of cryoprotectant, to controllable mode adjustment exchange in oocyte water and cryoprotectant, reduce the pressure or organelle and intracellular membrane system damage of cell structure of osmotic pressure shock to cell, improve the survival rate of cell.

To provide a method for culturing avian embryos outside the eggshell that can reliably achieve high embryo survival rates and low embryonic developmental abnormality rates, and that is relatively easy to perform, as well as a method for evaluating the toxicity of test substances using such a culture method. [Solution] The embryo and yolk are removed from the fertilized bird egg and transferred together with avian aqueous oocyte white into a roughly cylindrical well in a culture vessel. The opening of the well, which is filled with avian aqueous oocyte white, is then covered with a porous membrane, and the avian embryo is cultured by turning it over. Preferably, the cultured avian embryo is further cultured in a roughly cylindrical well in which a porous membrane is provided along the inner circumference, and the amount of avian aqueous oocyte white is adjusted so that there is a space between the well and the avian yolk below the opening.