219 results about "Oocyte" patented technology

The invention relates to the technical field of assisted reproduction polygene detection, and discloses an oocyte occurrence and maturation disorder detection panel, a detection kit and application thereof, and the detection panel comprises mutation genes related to the oocyte occurrence and maturation disorder for detection, copy number variation genes and rearrangement event and deletion genes. According to the application, by detecting high-risk genes and mutation sites, the risk of occurrence and maturation disorder of the oocytes is predicted in combination with clinic, the development potential of the oocytes is evaluated, the in-vitro maturation strategy is optimized, and the clinical outcome of assisted reproduction is improved. The detection panel can be used for efficiently detecting gene mutation which has clinical diagnosis and treatment significance on oocyte occurrence and maturation disorders; the method has important guiding significance on genetic counseling, risk prediction, clinical diagnosis, precise treatment and potential personalized intervention measures of patients suffering from recurrent oocyte occurrence disorder, oocyte maturation disorder and low oocyte maturation rate and patients to be subjected to assisted reproduction technology treatment.

The invention discloses application of an ACSL4 inhibitor in preparation of a medicine for improving quality reduction of an elderly ovum, and belongs to the technical field of biological medicines. The invention provides an application of targeting long-chain fatty acid acyl-coenzyme A synthetase 4 (ACSL4) in intervention of low oocyte quality and related fertility decline caused by aging. The ACSL4 inhibitor can be used for preparing drugs for improving ovarian functions and improving oocyte quality. In-vivo and in-vitro experiments prove that by inhibiting the ACSL4, the aging process of the elderly ovary can be effectively relieved, the expression of aging-related proteins p16 and p21 can be reduced, the mitochondrial function of the oocyte can be improved, and spindle assembly abnormality and chromosome arrangement defects can be rescued, so that the maturation rate and the development potential of the oocyte can be remarkably improved.

The invention discloses a bovine oocyte in-vitro maturation culture solution as well as a preparation method and application thereof, and belongs to the technical field of animal reproduction and embryo engineering. The preparation method of the bovine oocyte in-vitro maturation culture solution comprises the step of adding mitoquinine mesylate into a conventional IVM culture solution, wherein the adding concentration of the mitoquinine mesylate is preferably 50 nmol / L. The bovine oocyte in-vitro maturation culture solution provided by the invention can effectively reduce the oxidative stress reaction of the oocytes in the in-vitro culture process, enhance the mitochondrial function and remarkably improve the bovine oocyte in-vitro maturation quality and the embryonic development potential, so that the blastocyst rate is increased, and the problem that the development potential of the existing oocyte IVM culture solution is insufficient is solved.

The invention relates to the technical field of animal husbandry, in particular to a synchronous oestrus-timing insemination method suitable for cattle, which comprises the following steps: firstly, performing intramuscular injection of PG (cloprostenol sodium) at different time points after calving to promote uterine recovery and luteolysis and lay a synchronous oestrus foundation; the method comprises the key steps that GnRH (gonadotropin release hormone) is given through intramuscular injection on the 43rd day, the 53rd day, the 60th day and the 69th day, pituitary is promoted to release follicle-stimulating hormone (FSH) and luteinizing hormone (LH), collection and screening of follicles are guided, follicle maturation is promoted, the oestrus uncovering rate and the oestrus conception rate are increased, a melatonin implant is implanted in a subcutaneous implantation mode on the 63rd day, and the melatonin implant is implanted in a subcutaneous implantation mode on the 63rd day. The synthesis of ovarian hormones is promoted by regulating the hypothalamus-pituitary-gonad axis, the secretion of follicle cell factors and maturation promoting factors is promoted, the development of granular cells is promoted, and the maturation of oocytes is promoted. According to the method, the pregnancy rate of a double-synchronization-timing insemination scheme which is most widely used in a large-scale dairy cow breeding pasture at present can be remarkably increased, and feasible technical support is provided for success of increasing the breeding efficiency of cows.

This invention relates to the fields of molecular biology and biomedicine, and discloses siRNA for the expression of the *Schistosoma japonicum* Hsc20 gene and its applications. The siRNA contains a nucleotide sequence that hybridizes to a target sequence of the *Schistosoma japonicum* Hsc20 gene, wherein the target sequence is selected from the sequences shown in SEQ ID NO. 1-SEQ ID NO. 4. The siRNA specifically inhibiting the expression of the *Schistosoma japonicum* Hsc20 gene provided by this invention can be used to interfere with the transcription and expression of the *Schistosoma japonicum* Hsc20 gene and the growth and development of *Schistosoma japonicum*. In vitro experiments have confirmed that the siRNA provided by this invention can efficiently silence the *SjHsc20* gene; and in vivo RNA interference experiments in mice show that this siRNA can induce 50.62% (…) P The insect reduction rate was <0.05% and 44.29% ( P A liver oocyte reduction rate of <0.01% is suitable for preparing drugs to treat schistosomiasis.

The application discloses a kind of marker for predicting oocyte copy number variation and application thereof, the marker includes the combination of CLEC11A, P4HB, EFEMP2, IL32, FTL, FLNA, COL6A3, ACPP and APOO protein.By the marker of the application, the expression level of granulosa cell secretory protein can be determined by granulosa cell transcriptome sequencing, and the genomic CNV condition of corresponding oocyte can be inferred.The detection object of the technical solution is discarded granulosa cell in assisted reproductive technology, so it will not have any impact on oocyte, and it is non-invasive;The present study is a transcriptome and methylation group detection from single cell level, with high-throughput characteristics, and all granulosa cells and oocytes are one-to-one matched, which can reflect the difference between single cell level COCs.

The utility model discloses a flushing connecting device capable of efficiently operating and a double-cavity oocyte collector, the flushing connecting device comprises a flushing fluid suction tube, a hard connecting tube and a three-way connector, a second port of the flushing fluid suction tube is connected to the hard connecting tube, a first one-way valve is arranged at a first port of the three-way connector, and a second one-way valve is arranged at a second port of the three-way connector. A first port of the three-way connector is connected with a first port of a flushing fluid suction tube, a second port of the three-way connector is connected with an injector, and a third port of the three-way connector is provided with a second one-way valve and is connected with a flushing tube. The double-cavity oocyte collector comprises a double-cavity oocyte collecting needle tube, a washing tube, a liquid medicine filter and the washing connecting device, a first port of the washing tube is connected with a third port of the three-way connector, a second port of the washing tube is connected with a washing cavity of the double-cavity oocyte collecting needle tube, and the liquid medicine filter is arranged on the washing tube. The ovum extractor can be used more efficiently, the operation time can be remarkably saved, and the ovum extraction efficiency is improved.

An apparatus for diagnosing an embryo implantation potential using an artificial intelligence-based diagnostic model according to the present disclosure includes a memory storing a diagnostic model trained to determine an implantation potential index for an embryo based on an image generated by photographing one or more embryos formed by fertilization of a sperm and an oocyte and before implantation, and a processor for inputting a diagnostic image generated by photographing one or more first embryos, which are determination targets of the implantation potential index, into the diagnostic model as input data, and receiving implantation potential index data, which is a result of determining the implantation potential index for the first embryo, as output data from the diagnostic model.

The present invention relates to a medium composition for in vitro fertilization and / or in vitro culture comprising a compound represented by chemical formula 1 or a pharmaceutically acceptable salt thereof, and a method using same. The compound or composition suppresses reactive oxygen species and lipid peroxidation in ovarian granulosa cells to preventing cytotoxicity and mitochondrial dysfunction caused by apoptosis and ferroptosis, leading to an improvement in the quality of aged oocytes, and to increase the developmental rate and blastocyst formation rate of pre-implantation embryos, thereby improving the efficiency of in vitro fertilization. In addition, treatment with the medium composition during the vitrification process of in vitro–fertilized embryos improves re-expansion and survival rates, thereby reducing structural and metabolic damage. Accordingly, the present invention can be advantageously applied to an assisted reproductive technology for treating infertility and subfertility, and in vitro fertilization and / or in vitro culture for improving the propagation efficiency of livestock.

A microfluidic device that includes a trap configured to retain an oocyte or fertilized species, a plurality of microchannels in fluid communication with the trap, and a plurality of valves, where each valve is configured to control fluid flow through a corresponding set of one or more of the plurality of microchannels. The microfluidic device further includes a plurality of fluidic timer circuits each configured to produce a corresponding first time-dependent fluid pressure signal, and a fluidic logic gate circuit configured to receive the first time-dependent pressure signals and to produce a plurality of second time-dependent fluid pressure signals, where each second time-dependent fluid pressure signal controls operation of a corresponding one of the valves.

The invention discloses a method for promoting ovulation of sexual maturity zebrafish by using Gap27, and relates to the technical field of artificial breeding of fish, a gap connecting protein inhibitor Gap27 is applied to ovulation induction of zebrafish, an intercellular communication network in follicles is accurately intervened, the problem of asynchronous ovulation is solved, and in the final stage of maturation of oocytes, ovulation of sexual maturity zebrafish is promoted. Close substance and information exchange is carried out among follicular granule cells through a gap connecting channel formed by connecting protein Cx43, and Gap27 is used as a specific peptide fragment, can simulate an extracellular ring structure of Cx43, and competitively binds with Cx43 on an adjacent cell membrane, so that formation and functions of the gap connecting channel are physically blocked, and the follicular granule cells are separated from each other. When ovarian tissues of sexually mature female fish are exposed in a Gap27 solution, the transmission of inhibitory signals from granular cells to oocytes can be effectively interrupted, the rupture of nuclear membranes of the cells is promoted, and the final maturation process is completed.

The present invention relates to a double-lumen oocyte aspiration needle and use thereof in the collection of oocytes from the ovaries of animals undergoing assisted reproduction procedures.

The invention discloses application of spermidine in in-vitro culture of human unfrozen oocytes and embryos. By adding the combination of spermidine, melatonin and astaxanthin into the thawed oocyte resuscitation culture solution and the embryo in-vitro culture solution, the mitochondrial autophagy and mitochondrial functions of the thawed oocytes are improved, so that the in-vitro culture effect of the thawed oocytes and embryos is improved, and the success rate of an assisted reproduction technology is increased.

The present application relates to the technical field of computer vision, and particularly relates to an oocyte maturity analysis method, device and computer storage medium, the analysis method comprises the following steps: S1, classifying oocyte images into oocyte images with granulosa cells removed and oocyte images without granulosa cells removed; further classifying into mature and immature; S2, data augmentation is performed on the immature; S3, the oocyte images are segmented into non-overlapping image blocks, through four-stage processing, the feature maps of each image block in multiple dimensions are obtained; the weight scores of the feature maps in different dimensions are calculated in the dimension direction; the probability value of the category to which the image to be predicted belongs is output through a full connection layer, and the analysis of oocyte maturity is completed; the method of the present application has higher recognition preparation rate, faster speed, stronger device mobility, and has a significant effect on recognizing oocyte images before granulosa cells are removed.

The first objective is to identify previously unknown factors or combinations of factors that can efficiently produce oocyte-like cells from pluripotent stem cells, etc. The second objective is to provide oocyte-like cells and an efficient method for producing them. [Solution] A method for producing egg-like cells, which includes a step of introducing a factor into a cell, wherein the factor includes DLX6 or a nucleic acid encoding DLX6, and egg-like cells derived from human pluripotent stem cells and expressing PADI6.

The invention is suitable for the technical field of insect genetic engineering, and provides an HparGR22 gene and application thereof in establishing a phlorizin recognition in-vitro model. The DNA sequence of the HparGR22 gene is as shown in SEQ ID No: 1, and the coded amino acid sequence is as shown in SEQ ID No: 2. The gene has the highest expression quantity in the lower labial tassel of a holotrichia parallela larva, an oocyte injected with cRNA of the gene has obvious response to stimulation of phlorizin with different concentrations, it is clear that a product expressed by the HparGR22 gene can recognize phlorizin, and the function of the HparGR22 gene as the phlorizin receptor gene of the holotrichia parallela is determined. In view of the fact that the phlorizin recognition function of the gene is clear for the first time, a basis is provided for application of the gene in establishment of a phlorizin recognition in-vitro model, a foundation is laid for exploring the potential value of the gene in the field of gene engineering for preventing and controlling holotrichia parallela, and meanwhile a new direction is opened up for further research and application of the gene.

An oocyte retrieval tubing system comprising a manifold for receiving a supply of heated air and an outer tubing for accommodating an internal transfer tube that extends between a collection container and an oocyte retrieval needle in use, the outer tubing comprising first and second separate insulating tubes connected to each other via a manifold, the first and second insulating tubes each configured to be adjustable in length, whereby adjusting their working lengths relative to each other regulates the flow of heated air within the outer tubing received through the manifold disposed therebetween to maintain a stable and uniform temperature distribution within the outer tubing for oocyte retrieval.

The utility model relates to a kind of cell vitrification freezing automatic systems, including workbench;Including several injection pumps, the injection pump is installed on workbench, movable piston rod is provided in the injection pump, and the piston rod upper end is connected with driving assembly;Including fluid collecting valve, the fluid collecting valve is installed on workbench, and the fluid collecting valve is connected with the liquid outlet end of injection pump;Including frozen macaroni, the frozen macaroni and fluid collecting valve are connected;Including temperature control board, the temperature control board is installed on workbench, the utility model is advantageous in that system automatically controls the loading and unloading of cryoprotectant, to controllable mode adjustment exchange in oocyte water and cryoprotectant, reduce the pressure or organelle and intracellular membrane system damage of cell structure of osmotic pressure shock to cell, improve the survival rate of cell.

To provide a method for culturing avian embryos outside the eggshell that can reliably achieve high embryo survival rates and low embryonic developmental abnormality rates, and that is relatively easy to perform, as well as a method for evaluating the toxicity of test substances using such a culture method. [Solution] The embryo and yolk are removed from the fertilized bird egg and transferred together with avian aqueous oocyte white into a roughly cylindrical well in a culture vessel. The opening of the well, which is filled with avian aqueous oocyte white, is then covered with a porous membrane, and the avian embryo is cultured by turning it over. Preferably, the cultured avian embryo is further cultured in a roughly cylindrical well in which a porous membrane is provided along the inner circumference, and the amount of avian aqueous oocyte white is adjusted so that there is a space between the well and the avian yolk below the opening.

The invention discloses design and synthesis of a hapten of cyromazine, development of a cyromazine artificial antigen and a cyromazine antibody, and application of the cyromazine artificial antigen and the cyromazine antibody in the field of food safety rapid detection. The aminocarboxylic acid modified cyromazine hapten product is prepared through the steps of cyromazine hapten design, connecting arm introduction, synthesis, purification and the like. The cyromazine hapten with the structure is coupled with keyhole limpet hemocyanin to form an artificial immune antigen with immunogenicity, and is coupled with chicken ovalbumin to form an artificial competitive antigen. Tests show that the artificial immune antigen has high immunocompetence and can stimulate an animal immune system to generate an anti-cyromazine antibody, and the titer of an immune Blab / c mouse serum antibody is 16000. The IC50 of an enzyme-linked immunosorbent assay (ELISA) detection method established by the cyromazine artificial competitive antigen and the anti-cyromazine antibody is 5.3 [mu] g / L, and the detection sensitivity of an established gold-labeled immunochromatography detection method reaches 50 [mu] g / L.

The application belongs to the technical field of biology and particularly relates to a functional recombinant Cas9 protein targeted to an ovocyte of Procambarus clarkii and application thereof. The protein is NLs-VgSP-Cas9-NLs. The application directly delivers the recombinant Cas9 protein, thereby reducing the risk of integration of exogenous genes. By specifically targeting the ovocyte, off-target effects on somatic cells can be avoided, the efficiency of editing of germ cells is improved, and early development or genetic manipulation can be conveniently studied. The ovocyte of Procambarus clarkii is large, the recombinant Cas9 protein is efficiently delivered by receptor-mediated endocytosis on the surface of the ovocyte, and the problem that microinjection of zygotes cannot be implemented is solved.

The invention discloses an extract for promoting health and application thereof, and belongs to the technical field of medical and edible dual-purpose and traditional Chinese medicine health care, and the extract is a special extract obtained from beautiful millettia roots. The beautiful millettia root extract exerts new activity for promoting female health through a new mechanism, and the clinical effect is obviously improved. Experimental research proves that the extract promotes self-renewal of female germline stem cells and directional differentiation of the female germline stem cells into oocytes by promoting expression of Notch1, HES1 and HES5 in a Notch signal channel and regulates the female progestational hormone level, and shows the effects of promoting and recovering development of a second sexual character and preventing symptoms of polycystic ovarian syndrome on ovarian development disorder, and has a good application prospect. The radix millettiae speciosae extract is capable of promoting female physical development and maturation, delaying premature ovarian failure, relieving female depression and anxiety states and keeping female health, belongs to a new target and new mechanism, and makes up the mechanism blank of the radix millettiae speciosae extract in the aspect of keeping female health. The application has innovativeness.

The invention discloses a method for synergistically improving the activity of porcine oocytes through low-temperature plasma and erythritol, and belongs to the technical field of reproductive biology. The method comprises the following steps: collecting pig ovaries and separating oocytes; freezing protection is carried out by adopting a freezing liquid (DMSO: EG: FSF = 2: 2: 6) containing 0.5 mol / L erythritol, and preservation is carried out at-80 DEG C for 48 hours; and after unfreezing, treating for 30 seconds by using low-temperature plasma (6.83 kHz, 4.8 kV) to activate the cells. Experiments show that the erythritol can reduce frozen ice crystal damage, and the plasma treatment can significantly improve the cell activity after resuscitation, reduce the ROS content, increase the GSH reserve and enhance the mitochondrial membrane potential. And the survival rate of the oocytes is improved through synergism of the two. The problems that the cell activity is reduced and oxidative damage is serious in the cryopreservation process are solved, and an efficient technical scheme is provided for preservation of pig germplasm resources.

The utility model relates to the technical field of incubators, in particular to an electric heating constant-temperature incubator for AOI oocyte in-vitro activation, which comprises an incubator, a driving mechanism is arranged on the inner surface of the incubator, the driving mechanism comprises a fork-shaped fixing frame, the fork-shaped fixing frame is fixedly connected with the inner surface of the incubator, and the fork-shaped fixing frame is fixedly connected with the inner surface of the incubator. A movable frame is slidably connected to the inner surface of the fork-shaped fixing frame, a limiting rod is fixedly connected to the upper surface of the fork-shaped fixing frame, a push rod penetrates through the front surface of the limiting rod and is slidably connected with the front surface of the limiting rod, and the push rod is fixedly connected with one end of a first spring; through the arrangement of the fork-shaped fixed frame, the push rod, the first spring and the movable frame, when the movable door slides rightwards and the incubator is opened, the push rod is driven by the elastic force of the first spring to push the movable frame to automatically face outwards, so that researchers can conveniently take cells in the incubator, meanwhile, when the movable frame pops up, the movable frame can be slowly moved out, and the operation is convenient. And the incubator is more stable.

The application relates to the field of biotechnology, and particularly relates to an application of Bacteroides ovatus in ovarian aging; the application comprises: taking low-abundance Bacteroides ovatus as a biomarker of ovarian aging; the application comprises using Bacteroides ovatus in a medicine for delaying ovarian aging; the application further comprises using a fermentation product of Bacteroides ovatus in the medicine for delaying ovarian aging; it is found through experiments that there is a significant difference in Bacteroides ovatus in mice with different degrees of ovarian aging, and it is found through subsequent experiments that Bacteroides ovatus has the effects of significantly increasing the number of oocytes, up-regulating anti-Mullerian hormone, up-regulating the level of estradiol and down-regulating the level of follicle-stimulating hormone, which indicates that Bacteroides ovatus can be used in the medicine for delaying ovarian aging.

The application discloses a method for promoting ovary development of Pelteobagrus vishnui, belongs to the technical field of fish culture, and feeds a functional additive during an ovary development preparation period and a strengthening period in the process of culturing Pelteobagrus vishnui, wherein the functional additive is a microcapsule prepared by mixing epigallocatechin gallate and a composite unsaturated fatty acid, embedding the inside layer by using beta-cyclodextrin, and coating the outside layer by using chitosan; and the composite unsaturated fatty acid is composed of monounsaturated fatty acid and polyunsaturated fatty acid at a volume ratio of 0.8-1:1-1.2. In the application, the EGCG and the composite unsaturated fatty acid are compounded, and then embedded to prepare the microcapsule, which is added into feed for feeding, so that the ovary development of Pelteobagrus vishnui is effectively promoted, the number of oocytes is increased, the proportion of III stage oocytes is increased, the fecundity of Pelteobagrus vishnui is effectively improved, and the spawning quantity, fertilization rate and hatching rate are increased.

The present invention relates to the technical field of genetic transformation of insect eggs. Specifically, the present invention refers to an efficient genetic editing system to obtain recombinant or genetically modified insect eggs, by incorporating genetic material directly into oocytes of female insects, which will then generate a large number of eggs with the incorporated or recombinant genetic material.

The present application relates to the technical field of medical apparatus and instruments, in particular to a double-cavity oocyte extraction needle foot pedal quantitative flushing operation device, which comprises a quantitative liquid supply box, the quantitative liquid supply box is connected with a treading mechanism for foot treading control; the quantitative liquid supply box is provided with a placing cavity, the bottom end of the placing cavity is provided with a transfer assembly, the transfer assembly extends out of the quantitative liquid supply box and communicates with a flushing cavity, and the transfer assembly is connected with the treading mechanism in control; the transfer assembly comprises a liquid taking needle arranged at the bottom end of the placing cavity, the outlet of the liquid taking needle communicates with a transfer cavity arranged in the quantitative liquid supply box, the liquid taking needle is inserted into a liquid storage cylinder placed in the placing cavity, and the inlet of the liquid taking needle communicates with the inner cavity of the liquid storage cylinder. The device is compact in structure, closely connected between components and reasonable in design, reduces the operation pressure in the use process, improves the safety of the device, enables medical staff to easily start and quickly master the operation method, and is also convenient for daily maintenance and cleaning work.

The invention belongs to the technical field of animal reproductive biology and cell engineering, and particularly relates to a fluorescence labeling expression system for visualizing an oocyte nucleus maturation process and application of the fluorescence labeling expression system. The fluorescence labeling expression system comprises an eukaryotic expression vector pVenus-LMNB1; the eukaryotic expression vector pVenus-LMNB1 comprises a nucleotide sequence for coding the fusion protein Venus-LMNB1. According to the invention, a nuclear fiber layer protein stably expressed on a nuclear membrane is selected as a research object, a eukaryotic expression vector pVenus-LMNB1 is constructed by using a molecular cloning technology, an LMNB1 gene containing a green fluorescent label Venus is overexpressed in a porcine oocyte by using a microinjection technology, fluorescent protein labeling of a structural protein on the nuclear membrane is utilized, and the expression vector pVenus-LMNB1 is constructed. The expression positioning condition of the LMNB1 in the maturation process of the porcine oocytes is observed, and a powerful tool is provided for development of in-vitro culture related researches of the porcine oocytes.